LOCUS       DBNIBO010000001        69828 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.001, whole genome shotgun sequence.
ACCESSION   DBNIBO010000001 DBNIBO010000000
VERSION     DBNIBO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 69828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.001"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(111..344)
                     /locus_tag="AC1Y77_000001"
     CDS             complement(111..344)
                     /locus_tag="AC1Y77_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1573614.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            527..2197
                     /locus_tag="AC1Y77_000002"
     CDS             527..2197
                     /locus_tag="AC1Y77_000002"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIQ1573615.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            2344..2730
                     /locus_tag="AC1Y77_000003"
     CDS             2344..2730
                     /locus_tag="AC1Y77_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573616.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            2717..3415
                     /locus_tag="AC1Y77_000004"
     CDS             2717..3415
                     /locus_tag="AC1Y77_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIQ1573617.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNMLG
                     IVRPRDIYKQIEKD"
     gene            3420..3884
                     /gene="lspA"
                     /locus_tag="AC1Y77_000005"
     CDS             3420..3884
                     /gene="lspA"
                     /locus_tag="AC1Y77_000005"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIQ1573618.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            3881..4792
                     /locus_tag="AC1Y77_000006"
     CDS             3881..4792
                     /locus_tag="AC1Y77_000006"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIQ1573619.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            5027..5566
                     /gene="pyrR"
                     /locus_tag="AC1Y77_000007"
     CDS             5027..5566
                     /gene="pyrR"
                     /locus_tag="AC1Y77_000007"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIQ1573620.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            5580..6863
                     /locus_tag="AC1Y77_000008"
     CDS             5580..6863
                     /locus_tag="AC1Y77_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIQ1573621.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            6876..7802
                     /locus_tag="AC1Y77_000009"
     CDS             6876..7802
                     /locus_tag="AC1Y77_000009"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIQ1573622.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            7821..9101
                     /locus_tag="AC1Y77_000010"
     CDS             7821..9101
                     /locus_tag="AC1Y77_000010"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIQ1573623.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            9106..10182
                     /locus_tag="AC1Y77_000011"
     CDS             9106..10182
                     /locus_tag="AC1Y77_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIQ1573624.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            10187..13372
                     /gene="carB"
                     /locus_tag="AC1Y77_000012"
     CDS             10187..13372
                     /gene="carB"
                     /locus_tag="AC1Y77_000012"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIQ1573625.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            13426..14202
                     /locus_tag="AC1Y77_000013"
     CDS             13426..14202
                     /locus_tag="AC1Y77_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIQ1573626.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            14199..15131
                     /locus_tag="AC1Y77_000014"
     CDS             14199..15131
                     /locus_tag="AC1Y77_000014"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIQ1573627.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            15128..15844
                     /gene="pyrF"
                     /locus_tag="AC1Y77_000015"
     CDS             15128..15844
                     /gene="pyrF"
                     /locus_tag="AC1Y77_000015"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIQ1573628.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            15851..16480
                     /gene="pyrE"
                     /locus_tag="AC1Y77_000016"
     CDS             15851..16480
                     /gene="pyrE"
                     /locus_tag="AC1Y77_000016"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIQ1573629.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIETALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            16468..16782
                     /locus_tag="AC1Y77_000017"
     CDS             16468..16782
                     /locus_tag="AC1Y77_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573630.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            16820..17461
                     /locus_tag="AC1Y77_000018"
     CDS             16820..17461
                     /locus_tag="AC1Y77_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HIQ1573631.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            17504..18196
                     /locus_tag="AC1Y77_000019"
     CDS             17504..18196
                     /locus_tag="AC1Y77_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HIQ1573632.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            18193..19095
                     /locus_tag="AC1Y77_000020"
     CDS             18193..19095
                     /locus_tag="AC1Y77_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1573633.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(19253..20959)
                     /gene="efbA"
                     /locus_tag="AC1Y77_000021"
     CDS             complement(19253..20959)
                     /gene="efbA"
                     /locus_tag="AC1Y77_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HIQ1573634.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            21149..21772
                     /locus_tag="AC1Y77_000022"
     CDS             21149..21772
                     /locus_tag="AC1Y77_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573635.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            22222..23271
                     /gene="trpX"
                     /locus_tag="AC1Y77_000023"
     CDS             22222..23271
                     /gene="trpX"
                     /locus_tag="AC1Y77_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1573636.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     LDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            23268..24149
                     /locus_tag="AC1Y77_000024"
     CDS             23268..24149
                     /locus_tag="AC1Y77_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1573637.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            24152..24922
                     /locus_tag="AC1Y77_000025"
     CDS             24152..24922
                     /locus_tag="AC1Y77_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573638.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            25201..26055
                     /gene="aroE"
                     /locus_tag="AC1Y77_000026"
     CDS             25201..26055
                     /gene="aroE"
                     /locus_tag="AC1Y77_000026"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIQ1573639.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            26233..27396
                     /locus_tag="AC1Y77_000027"
     CDS             26233..27396
                     /locus_tag="AC1Y77_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIQ1573640.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            27513..27923
                     /locus_tag="AC1Y77_000028"
     CDS             27513..27923
                     /locus_tag="AC1Y77_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1573641.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            28097..30325
                     /gene="topB"
                     /locus_tag="AC1Y77_000029"
     CDS             28097..30325
                     /gene="topB"
                     /locus_tag="AC1Y77_000029"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIQ1573642.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPHTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            30496..30780
                     /locus_tag="AC1Y77_000030"
     CDS             30496..30780
                     /locus_tag="AC1Y77_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573643.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            30940..31176
                     /locus_tag="AC1Y77_000031"
     CDS             30940..31176
                     /locus_tag="AC1Y77_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1573644.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            31173..31571
                     /locus_tag="AC1Y77_000032"
     CDS             31173..31571
                     /locus_tag="AC1Y77_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIQ1573645.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(32238..33182)
                     /locus_tag="AC1Y77_000033"
     CDS             complement(32238..33182)
                     /locus_tag="AC1Y77_000033"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIQ1573646.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(33478..34422)
                     /locus_tag="AC1Y77_000034"
     CDS             complement(33478..34422)
                     /locus_tag="AC1Y77_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIQ1573647.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            34643..35857
                     /locus_tag="AC1Y77_000035"
     CDS             34643..35857
                     /locus_tag="AC1Y77_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1573648.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            35879..37894
                     /locus_tag="AC1Y77_000036"
     CDS             35879..37894
                     /locus_tag="AC1Y77_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HIQ1573649.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(37977..39350)
                     /locus_tag="AC1Y77_000037"
     CDS             complement(37977..39350)
                     /locus_tag="AC1Y77_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIQ1573650.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(39376..39660)
                     /locus_tag="AC1Y77_000038"
     CDS             complement(39376..39660)
                     /locus_tag="AC1Y77_000038"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1573651.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(39662..41677)
                     /locus_tag="AC1Y77_000039"
     CDS             complement(39662..41677)
                     /locus_tag="AC1Y77_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1573652.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(41856..42161)
                     /locus_tag="AC1Y77_000040"
     CDS             complement(41856..42161)
                     /locus_tag="AC1Y77_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HIQ1573653.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(42250..43281)
                     /locus_tag="AC1Y77_000041"
     CDS             complement(42250..43281)
                     /locus_tag="AC1Y77_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIQ1573654.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            43488..44363
                     /locus_tag="AC1Y77_000042"
     CDS             43488..44363
                     /locus_tag="AC1Y77_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1573655.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(44515..45357)
                     /locus_tag="AC1Y77_000043"
     CDS             complement(44515..45357)
                     /locus_tag="AC1Y77_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1573656.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLSKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(45449..47326)
                     /locus_tag="AC1Y77_000044"
     CDS             complement(45449..47326)
                     /locus_tag="AC1Y77_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIQ1573657.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(47323..48744)
                     /locus_tag="AC1Y77_000045"
     CDS             complement(47323..48744)
                     /locus_tag="AC1Y77_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIQ1573658.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(48747..52136)
                     /locus_tag="AC1Y77_000046"
     CDS             complement(48747..52136)
                     /locus_tag="AC1Y77_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIQ1573659.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            52393..53784
                     /locus_tag="AC1Y77_000047"
     CDS             52393..53784
                     /locus_tag="AC1Y77_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1573660.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(53857..54504)
                     /locus_tag="AC1Y77_000048"
     CDS             complement(53857..54504)
                     /locus_tag="AC1Y77_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1573661.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(54512..55198)
                     /locus_tag="AC1Y77_000049"
     CDS             complement(54512..55198)
                     /locus_tag="AC1Y77_000049"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573662.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(55204..56277)
                     /locus_tag="AC1Y77_000050"
     CDS             complement(55204..56277)
                     /locus_tag="AC1Y77_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1573663.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            56499..57173
                     /locus_tag="AC1Y77_000051"
     CDS             56499..57173
                     /locus_tag="AC1Y77_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1573664.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            57178..58518
                     /locus_tag="AC1Y77_000052"
     CDS             57178..58518
                     /locus_tag="AC1Y77_000052"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1573665.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(58667..59653)
                     /locus_tag="AC1Y77_000053"
     CDS             complement(58667..59653)
                     /locus_tag="AC1Y77_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HIQ1573666.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            60377..61408
                     /locus_tag="AC1Y77_000054"
     CDS             60377..61408
                     /locus_tag="AC1Y77_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1573667.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            61517..63049
                     /locus_tag="AC1Y77_000055"
     CDS             61517..63049
                     /locus_tag="AC1Y77_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIQ1573668.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            63188..64000
                     /locus_tag="AC1Y77_000056"
     CDS             63188..64000
                     /locus_tag="AC1Y77_000056"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HIQ1573669.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            63990..64475
                     /locus_tag="AC1Y77_000057"
     CDS             63990..64475
                     /locus_tag="AC1Y77_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1573670.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            64498..65043
                     /locus_tag="AC1Y77_000058"
     CDS             64498..65043
                     /locus_tag="AC1Y77_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIQ1573671.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(65116..66759)
                     /locus_tag="AC1Y77_000059"
     CDS             complement(65116..66759)
                     /locus_tag="AC1Y77_000059"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIQ1573672.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(66888..69116)
                     /locus_tag="AC1Y77_000060"
     CDS             complement(66888..69116)
                     /locus_tag="AC1Y77_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1573673.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(69149..69628)
                     /locus_tag="AC1Y77_000061"
     CDS             complement(69149..69628)
                     /locus_tag="AC1Y77_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HIQ1573674.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        21557 a        12711 c        13913 g        21647 t
ORIGIN      
        1 agaaaaagct aattttaaaa atcagaacct aaaaaaacga atttttacag gaaaagaaaa
       61 gagtgagacg tacggttcat cccgtttagc gtctcactct atttgtttat ctattcatgc
      121 atactagtat cggtagtctg aacgttgcct ttcttgacat gggcagcttg tgttcctcga
      181 ttcccttcga tgatctcaaa ttcgaccact tgattttctt ccaaagtttt gaatccctct
      241 tcttcaatcg cagtaaaatg tacaaaaatt tcttctgtgt catcataact gataaagccg
      301 tatccttttt tgttgtcaaa ccattttaca attcctgtgg tcaaagcact cacgaccttt
      361 ctaaagtctg atcatagcct tcattataaa gagtatttat aagtacgtca aataaattaa
      421 aaacctgtaa ttttatgttt tttttctgaa taaaaaaagg tataattggc atatcttaac
      481 aaagaagata ttttgttgaa taaagttcgt gaaaaggggt aatcaaatga agaatgatct
      541 gcaaatttca caagaaacag agctagaacc gattgttgaa gtggctagta aattagatct
      601 taaggaagac gatttggaac tgtatgggaa atataaggcg aaaatcaatt tttctggaat
      661 caatcgatca aaggataacg ctgaggggca tttgatctta gttacttcca tcaatccaac
      721 accagctggc gaaggaaagt caacgatcac tgtagggtta ggagacgcgc tcaataaaat
      781 caataaaaaa tctgttattg cattgcgaga accctcactt ggtcctgtga tgggaatcaa
      841 aggtggtgct gcaggcggag gatatgcaca ggtattgcct atggaagata tcaacttgca
      901 ttttactggt gacatgcatg ctatcacaac agctaataat gcattatctg ctcttttaga
      961 taatcatatt catcaaggaa atgaattgaa catcgatgcc cgccgagtga tttggaaacg
     1021 ggtagtcgat ctcaatgatc gtgaactgcg taaagtaatt gttggcttgg gtgggccaat
     1081 ccaaggtgtt ccaagagaag atgggtttga tatcacagtt gccagcgaaa tcatggctat
     1141 cttgtgtctt gcggcagacc ttgaagattt aaaggctcgt cttgcaagga tcgttgtcgg
     1201 ctatacattt gaccgtcagc ctgtgactgc tggagacttg aaagcagaag gcgctctggc
     1261 attactgctg aaagatgcaa tcaagccgaa tcttgtacaa acaatttacg gaacaccagc
     1321 ttttgtccac gggggaccgt ttgccaatat cgctcatggc tgcaatagta ttttagctac
     1381 caagacagca ttgagattag cagattacgt tgtcacagag gcaggatttg gtgcagatct
     1441 tggcggagag aaatttttag atatcaaagt acctaacttg aaaaaagcgc cggatgctgt
     1501 ggtgattgtc gccactgtcc gtgcattgaa aatgcatggc ggaatgaaaa aagatgaact
     1561 taaaaatgag aatcttgatg ctttgaaagt aggttttgca aatttaaagc gccatatacg
     1621 taatatggaa caatatcagc tacctgtgat cgtcgcaatc aatgaattcg taacagatac
     1681 agacaatgaa ctcacactgt tggagcactt gtgtgaaaat caggggattc ttgccaagag
     1741 agcgagtgta tgggcagatg gagcagaagg cggcgttgat ttagcaaaag ctgtagtacg
     1801 attgattgat agaaaagaag ctaattacaa gccattatac cgcctagaag aaacgataca
     1861 agaaaagaca gaaacaatcg tcaagaaaat ttacggagga aatggtgttg ttttcagcaa
     1921 aaaagcgcaa aaacaaatag aagagtttac gaagaatgga tgggacaaac tgccgatttg
     1981 tatggcaaaa acacagtatt cattttctga tgatccttca ctgctaggtg caccggaaga
     2041 tttcaccatc acgatccgcg aaatcatccc aaaattaggg gcaggatttt tagtagcctt
     2101 gactggagat gttatgacga tgcctggttt gcctaaaaaa cctgctgcat tgaatatgga
     2161 cgtgacaaat gacggagaag tgttgggatt gttttaagct tgattataat ctaccatcga
     2221 aggagctgta ttttgactac agcttctttt tttagacgat agggaattaa ttttgtttac
     2281 actaggatca ttctttttct ttattgtatt ttgagctata ctaaagcata ataggagggt
     2341 atgatgaaaa cgacatttat aagaaaaacc ggtttttacg gtatgggtag ctcgattcag
     2401 ctaagaatca acaatgaaaa acgcagtgtt ctcagtcatg aacaaaagat aacgcttgat
     2461 ctggaagtgc cctttacgat gcaggtcacc ttttattggc taaaaagccc cgtttataca
     2521 gtgaaagaag caaagcctgc ttatgtgatc acaatgaatt acttgattct tcaaatctat
     2581 ccgtttttat ttctagcaac tgggttgggg gccgttttcc tacaaaattt atttttcagt
     2641 ttttttgcag ttattgtgtt gattgcgttc tttttctata taaaaaaccg ggcatatttg
     2701 atcaaggagg aaaattatgg caaactctga tcttttttta atcagtttca atcgaatcga
     2761 aaaatggatg agagacgaga tgggaaatgc acgaaatatg ggatttaccg aacttgtcag
     2821 acgcctagct caaagaaaac aattaatgat cagaaaatac gaagatgatc ttttacagct
     2881 tgctcagtta aggaatgcga tcgtccatga tcggatcgca gtcgattttg tcattgcaga
     2941 gccaaatgat tggacagtca agcgaatcca gctgatcgaa caagaactga ttcagcctga
     3001 aaccgtgctg cctcgatttg caaaacgtgt aacaggtttt gaacaagatc ttccgttact
     3061 tgaactgtta aagatagtag cagaaaaaag gtattcacaa tttccgttat acaataaagg
     3121 aaaatttgta gcattgatta ctttacgtaa tatcgggttt tggctagcaa aagaaagcca
     3181 aaaaggtccg gttgatttaa caaataaacg agcgttggat ttgatcatcc aaaacggaaa
     3241 gtacacaaat tatcattttg ttccagctaa aacacatatt tacgaggttg aagcgatgtt
     3301 tcgtgagcaa gggactttgg aagccgtact tatcactaaa gatgggaatc ccgatggtaa
     3361 tatgctcggg atcgtacgac ctcgtgatat ttataaacaa atagaaaagg attgattttt
     3421 tgttaattat ttattggata ttcagcgctt tgctagtagg agtggatcag ttggtcaaat
     3481 ggtggatcac tgataatcta gcgcttggag agacgaaaaa tcttatacca ggtattttgt
     3541 cactgaatca tattcgaaac actggagccg catggagtat gctagaaggg aagatgtggt
     3601 tctttactat agtgacactc attgcagtcg ttgtgattct cacattgatg attaaaaatc
     3661 gtgaaaaagg taatcgctgg ttcatgattg gattgagttt gattctagct ggtgcgattg
     3721 gcaattttat cgaccgtatt cgtcttggct atgtagtaga tatgttccag acagatttta
     3781 tgagctttcc gatttttaac gtagcagatg tgacacttgt atgtggagtg atttgtatgt
     3841 taatttatat tattttagat gaaaaggatc aaagaaaaaa atgacagaac ttaaaacgac
     3901 gattcaccat gaatctggta ggatcgataa agtattgact aatttattca ccgattacag
     3961 tcgttcgcaa attcagctgt ggctgaaaaa tggagcggtt tctgtcaatg gacagccagt
     4021 caaagcaaat tacaaagtaa aacaaggaga cgagattact gtcttgatcc ccgaaccaga
     4081 aacattagat gtggtcccag aaaatatccc gttgacgatc gtttacgaag atgaagatgt
     4141 agcggtgata aacaaacctc aaggaatggt cgttcatcct tcagcaggtc atacgcatgg
     4201 cacaatggtc aatgcgctga tgtaccatat caaggatctt tctacgatca acggcgttat
     4261 tcgtccaggt attgttcacc gaatcgacaa agatacttcc ggactgctaa tggttgccaa
     4321 aaatgaccag gcacacgaat cattagctca gcaattaaaa gataagacct ctttgcgcaa
     4381 atatgttgct ttagtccatg gagagattcc tcacgaaaaa ggaaccatca atgcgccaat
     4441 cggccgttcg aaggaaaacc ggaaaatgca ggcgataaga gaagatggga agtcggctgt
     4501 cacacatttt actgtattgg aacgtttcga aggatttact ctggttgaat taaaactaga
     4561 aaccggaaga acacaccaga tccgtgtgca tatgaagtat atcggttatc cgttagctgg
     4621 agatccagta tacggaccga agaaaacttt gaaaggcaat ggtcagtttc tccatgcgca
     4681 aatgttaggt tttacgcacc cgactacaaa cgaaaagatg atctttgaag cacctcttcc
     4741 agaagttttt gaaaaaactt tagaaaaatt aagaaaaaac attgcatttt aatttagata
     4801 acggtatagt aattaacaac gaaagcataa aacttaatag aattccttta agtttagtcc
     4861 cgtgaggcta agaagggtgg gagcaaggat acgtatggtg cgccataagt agattgctca
     4921 ttgacatagt atagctatca gtgtatcagt atacgctcag tctaaacatt cctcctgtcc
     4981 tcttagggca ggagtttttt tatgcaaaaa agtaggggga atgaaaatgc aagcaaaaga
     5041 agtagtagat caggtaacca tgaagcgagc attgactcga attacttatg agattatcga
     5101 gagaaaccat agtatccaag atatcgtatt ggtaggtatc aaaacacgag gaatctacat
     5161 tgcatcaaga attgccgaac gcctgaagca gctagaagat atcgatattc ctgtaggaga
     5221 actggatatc actttatacc gagatgacaa aaaagaaaat ccagaagaac cggaacttca
     5281 ttcttctgac atcccagttt ccttagaagg aaaagaagtt attttgattg atgatgtttt
     5341 gtatacagga cgaacgattc gagcagcaat ggacgcagta atggattttg gcagaccaag
     5401 aaaaatttca ttagcagtcc tggtcgatcg tgggcatcga gaattgccaa tccgtgcaga
     5461 ttatgttggg aagaacattc caacatctcg ggcggaagag attttagtag agatgcaaga
     5521 gcttgatgga caagatcgca tcatgatttt gaaagaggaa gattagagga gagagaaaca
     5581 tgtcggagaa aaaaggattt cataatgatg acgtggtgtt agatatccac gatcgcccaa
     5641 ccccaggaca ttgggtagga ttaagtttac aacacttgtt cacgatgttc ggagcaaccg
     5701 tgttagtccc catcttagta ggtatcgatc caggtattgc gctagtcagt tcaggactag
     5761 gtacattggt gtacttattt actactaaag gaaagatacc tgcgtactta ggaagcagct
     5821 tcgctttcat tgcagcgatg cagcttttga tgaaaacaga tggttatcca gcgattgcac
     5881 aaggagcagt cacaactgga ttagtctatt tgattgttgc gctgatcatc aaaaagatcg
     5941 gttcagcatg gctggataaa atcttgcctc caatcgtcgt cggtccagtc atcatggtta
     6001 tcgggttggg acttgcggca aatgcagcaa caaatgctat gtataatcag ggcacgtatg
     6061 atttcaagta tattgctgta gcgctgatca cattaggatt aacaattttc ttcaacatgt
     6121 ggctaagagg ttttctcgga ttgattccga ttttactagg tattattagc ggatacatcg
     6181 tagccttgct gtttggaatc gtggatacta agccaatcgt agacgctgcc tggtttgctg
     6241 ttcctaactt tgagattccc tttgtacaat atgaaccaaa gctatatctt ggagcaatca
     6301 caacaatggc acccatcgct tttgtgacga tgacagagca tatcggccat cttatggtat
     6361 tgaataaact aactaaacgt aatttctttg aagaaccggg tcttcataag acgttgacag
     6421 gtgatggatt agcacaaatc gtggcagcct ttgtcggagg tcctcctgta acaagttacg
     6481 gtgaaaacat tggtgttctg gctatcacac gtgtccatag cgtgtttgtg atcggtggag
     6541 ctgcagtatt tgcagtcatt ttaggattcg taggaaaatt aagcgctttg attttaagta
     6601 tccctggacc agtcatctca ggaatcagtt ttatcttatt tggagtaatt gccgcaagtg
     6661 gaatgaagat tttaatcgag aacaaaatcg attttgataa aaagaaaaat ctgttgattg
     6721 cttcagttat cttagtagta ggaattggcg gactcgtctt tgaagtaggc acatttacat
     6781 tgtcggcaat ggcattagca actgtacttg gcatcatttt aaatcttatc ttaccagaaa
     6841 cttcacgaag cgaagaaaac taaggaggaa acattatgat catcaaatca gaacgtatca
     6901 gcttaaagca tttattgacc gttgaggcac ttactgatca agaagttatg ggcttgatcc
     6961 gcagagggca ggaattcaag caaggtgcta attggactcc gcaaaagcgc cagtactttg
     7021 ctacaaacct ctttttcgaa aatagtactc gcacacataa aagcttcgat gtagcggaga
     7081 aaaaactagg gctagaagtg attgaatttg aagcaagcac aagctctgtc caaaaagggg
     7141 aaacactata cgatacagta ctgacgatgt cagcattagg cgtagacgta gcggttatcc
     7201 gtcatggtga tgagaactat tatgacgaac tgatacaaag taaaacaatc cagtgttcga
     7261 ttatcaatgg aggagacgga agcggacagc atccaaccca atgcttgctt gatttgatga
     7321 ccatttatga agaatttggt cattttgaag gtctgaaagt tgccatcgta ggtgatatca
     7381 ctcactctcg cgtagctaaa tccaatatgc agatgttaaa acgtctaggc gcacaagtat
     7441 tcttctcagg tcctagagaa tggtacgacg atgaattcga agtgtacgga cattatatgc
     7501 cattggatga actactagat caagttgatg tcatgatgat gcttcgggtg caacacgaac
     7561 gccatgatgg aaaagaaagt ttctcaaaag aaggctacca tcaggaacat gggttaacag
     7621 ttgagcgtgc gaagaagttg caaaaacatg caatcattat gcacccagca ccagttaacc
     7681 gtgatgtaga attagctgat tcattagtgg aaggattaca gtcgcggatc gttcagcaaa
     7741 tgagcaatgg agtattcgtc agaatggcaa tcttagaagc agtgctaaat ggaaaagcgt
     7801 aattatagga ggatgaaaaa atgaaaacac tcattaaaaa cggtcagatc aatacacgaa
     7861 aaaatatgac gacaccagca gaaatttgga tagaagatgg gcgaatcaaa gcaatcggta
     7921 caggattctc agaagccgaa tttgatgaag tgtttgatgc aaagggacaa ttgatcacac
     7981 ctggtttagt ggacgtccat gttcatttga gagagccagg atttacatac aaggagacga
     8041 tcgaagctgg gactcgctca gctgctcgtg gcggtttcac tactgtatgc gcgatgccga
     8101 atttaaatcc tgtacctgat acggcagaaa agctgagaca agtatatgac atcattagaa
     8161 aagatgcagt tgtgaaggta ttgcagtatg cgccaatcac agaaaatctg cgaagtgaga
     8221 aattagttga tcaagaagca ctgattgaag aaggtgcttt tgctttcaca aatgacggtg
     8281 taggtgtcca aacagctggg acgatgtatc tggctatgaa agaagctgca aaaaacaata
     8341 aagccctagt agcccataca gaagatgaat ctttattatt tggcggcgtt atgcacgctg
     8401 gcaagaaggc agaagaactt ggtcttcccg ggatcttaag cgtaaccgag tcttctcaaa
     8461 ttgctagaga tctgttgcta gctgaagcaa ctggtgttca ttaccatgtt tgccatgttt
     8521 ctactaaaga aagtgtccgt gtaatcagag acgcaaaaaa agcaggaatc cacgtaacag
     8581 cagaagtttc accccatcat cttattttga tcgacgaaga tattcccgaa gatttcggct
     8641 tttggaaaat gaacccgccg ctaagaggac gggaggacag agaagctttg atcgaaggct
     8701 tgcttgacgg tacgatcgat tgcatcgcta cagaccatgc cccccatgga ttagaagaaa
     8761 aaagccaaag tttcatgaaa tcaccatttg gtatcgtagg aagcgaaaca gcgtttcaac
     8821 tgatctatac tcattttgta gaaactggtc gattcacgtt ggaacaagtg atcaattggt
     8881 tggcagttaa accggctgag atttttggtt tgaatgcagg aacgctaacg gttggtgccc
     8941 ccgctgatgt agcagttttt gatattacac aaacttgcac gatcgataaa gaagactttt
     9001 tatcaaaagg ggagaataca ccttttatcg gctggaaagt aaaaggcgaa acacaaatga
     9061 cttttgtcaa cggaaaactt gtgtggcaga agggagaata aaagtatgga acgtttgctt
     9121 atattagaag atggaacagt tttcgaagga aaggcttttg gtgcagatgc tagtgtcttc
     9181 ggagaaatcg tttttacaac aagtatgact ggttatcaag aaactatcac tgatcaaagt
     9241 tttaacggtc aaatcattac atttacctat cctatggtgg gtaattatgg ggtaaataga
     9301 gatgattatg aatcgatttc tccaacatgc aaaggagtag tagtaaaaga acatgcacgt
     9361 gtagcttcaa actggcgaaa ccaaatgacg ctagattcat ttttgaaaca aaaaggaatt
     9421 cctggaatat caggtatcga tacgcgcgcc cttactcgca aaatccgtga agtcggaacg
     9481 atgaaagcta gtatagttga tgctggagat tccttcgaac atgcatttga tcagctgaaa
     9541 gcaaccgttc tagcaaccaa tcaagtgcag caagtctcaa ctaacaaacc ttatccaagt
     9601 ccaggaacag gtcggaaagt cattgtcatt gacttcggct tgaaacatag tattttacga
     9661 gaattgagta aaagaaactg taacctcaca gtacttccct ataacacaga tgccgagacg
     9721 attttagcgc tatcaccaga cggagtcatg ctgacgaatg gacctggtga tccaaaagat
     9781 gtgccagaag caatcgaaat gatcaaaaaa atccaaggaa aagtaccgat ttttggtatt
     9841 tgtctcggtc accagttatt tgctttagcc aacggtgcag atacctataa aatgaaattc
     9901 ggacatcgag gattaaatca cccggtacga gaagtagcga ccggaagaat tgattttact
     9961 tctcaaaatc atggctatgc agtcgatgaa gccacgattg atccagaaaa actcatcgta
    10021 actcatgtag aagtaaatga cggaacagta gaaggagtca aacacagaga ttatcccgct
    10081 ttcagtgtgc agtttcatcc agatgctgcc cctggtccgc atgatgcact acatttattt
    10141 gatgaattta tggaaatgat ggacgcagga aaggagcagt aaacagatgc cgaaacgtac
    10201 agatatcaag aaaattatgg tgattggatc tggtccgatc atcattggtc aagccgcaga
    10261 atttgactac gcaggaacac aagcctgcct tgccttaaaa gaagaaggat atgaagtggt
    10321 tttagtaaat tctaatcctg cgacgatcat gacggataaa gaagtggcag atcaagtata
    10381 tatcgaaccg atcacacttg aatttgtatc aaggattctt cgaaaagagc gtccagatgc
    10441 tctccttcca actttaggcg gacagacagg cctcaatatg gcaatggagc tggcagcatc
    10501 aggtatttta gaagaattga acatagaact tctaggaaca aagctttctg cgatcgatca
    10561 agcagaagat cgtgatctat tcaaacagtt gatgaaagaa ttgaatcagc caatccccga
    10621 gtctgaaatc gtcaatacag tagaagaagc tgtagatttt gcaaataaaa tcggctatcc
    10681 tgtcatcgtt cgtccagcct ttactttagg cggaactggt ggaggtatgt gtgaaaatga
    10741 agaagaatta cgtatcgtat cagaaaatgg attaagtctt tcccccgcta cccaatgttt
    10801 gattgaacga agtattgcag gttttaaaga aattgaatat gaagtgatga gagattcggc
    10861 tgataatgca atcgttgtct gcaatatgga aaattttgac ccggtgggta tccatacagg
    10921 tgattctatc gtatttgccc ctagtcaaac attgtctgac catgaatatc aaatgcttcg
    10981 tgatgcttct ctttcgatta ttcgagcact taagatcgag ggaggctgta atgtccaact
    11041 cgctcttgat ccacatagtt ttaattatta tgtgattgaa gtcaatccgc gtgtttctcg
    11101 ttcatctgcg ttagcaagta aggcgactgg ctatccgatc gctaagctag ctgctaaaat
    11161 agcgcttgga ttaacattag acgaaatgaa aaatcctgtt acaggaacga cttttgcaga
    11221 atttgaacct gcattagatt atgttgtagc taaaattcct cgctggccgt ttgataagtt
    11281 tgaaaacgga gaacggcttt taggcacgca gatgaaagca actggtgaag tgatggctat
    11341 cggtcggaat atcgaagagt ctttattgaa agctgtgcgt tctttagaaa tcggtacaca
    11401 ccatatcgaa cttcctgagt tggcagacat cacggacgat gacctaatcc aaaaagtggt
    11461 tcgagcacaa gatgatcgcc tgttttatgt agctgaagct attagaagag ggtacaccat
    11521 agaagaatta gcggatttga caaaaatcga tttattcttt ttagataaac tgttgcatat
    11581 cgtagaaatc gaacagcagt tgaaacagca gccgttggat gacaagatat tgaaaacagc
    11641 gaaacaaaac ggttttacag acaaaaaagt ggcggagctg tgggaaataa acgaagaagc
    11701 cgttcgccag atgagaaaag aaaatgattt gattcctgta tataagatgg ttgacacttg
    11761 tgcagcggaa ttcgaatcaa aaacacctta tttttatagt tcctatgaat atgaaaatga
    11821 aagttcacga tcagaaaagc cctcaattct agtactggga tctggtccta tccgtatcgg
    11881 acaaggggtt gaatttgact atgcgactgt gcattctgtc aaagcaatcc agcaggcagg
    11941 atacgaagca atcatcatga acagcaatcc tgaaacagtt tcgactgact tttctatttc
    12001 ggataaatta tattttgagc cattgacctt cgaagatgtg atgaatgtga tcgagctaga
    12061 acagccgata ggagtaatcg ttcaatttgg tggtcagaca gcaatcaact tagccgaacc
    12121 tttgactgct gctggtgtaa aaatcttagg gacaacgatc gaagacttgg atcgtgcgga
    12181 aaatcgtgat ttattcgagc aagcgctgaa agagttggat attcctcaac ctttagggga
    12241 tactgctaca agtaaagaag aagccgtggt tatcgcttcg agaattggtt atccagttct
    12301 tgttcgtcca agctatgtat taggcggacg tgcgatggaa atcgttgaaa accagcgaga
    12361 tttagaagat tacatggaac atgctgtgaa agcatctcca gaacatccag tactagtgga
    12421 ccgttacttg cttggtggtg aatgcgaagt ggatgcgatt tgtgacggtg aaacagtact
    12481 catcccaggg atcatggagc atattgagcg tgctggtgtc cattcagggg attcaatggc
    12541 tgtgtatcca cctcaaacat tatctgaatc gatcaagaaa acgatcgaag attacacgat
    12601 ccgcttagca ttaggtttga attgtgtcgg gatgatgaat atccagtttg tcatccatga
    12661 aaatgaggtt tatgtgatcg aagtcaatcc tcgagcaagt cggactgttc cattcttaag
    12721 taaggtgaca ggaattccaa tggcacaaat tgcgacgaaa gctattttgg gagagaaact
    12781 aaagacactt ggttatgaaa acggacttta tccagaaaca aaagaagttc atgttaaagc
    12841 acctgtcttt tcttttacga aattgcagaa agttgatact tacttagggc ctgaaatgaa
    12901 atcgactgga gaagtaatgg gatctgacag acaattagac aaagctttgt acaaggcatt
    12961 cgaagcttct ggattacact tgcctgaata tggagccgta ttatttacga ttgcggacga
    13021 aacaaaagaa gaagcactag aattagctaa acgttttaca gaaattggct atagtctgat
    13081 aggaacgaaa catacagctg cttatttcga aaaaaatggt ctggtcgttg aaacagtagc
    13141 gaaaatttct gaggaagcag cagaaaaaaa tgtagtcgac ttaataagag atggtaaaac
    13201 acaagtggtt gtcaatacga ttgataaaga tcggggaaat gcttctaaag acggtttcat
    13261 catccgaaga gaagcagttg aacatggtat tccattgttt acttctttag acacagcaga
    13321 tgcgatcgtt cgagtgatgg aatcccgtgc tttttcaact aaccctattt aacggcgagc
    13381 acatgttaag acaaaaagac gaaaaaccgt tgcaggaggg gacagatgaa acaagaaatg
    13441 atggaaatcg ttcagcaaaa acagctggca ccaaagattt ttgaaatgac attgaaaggt
    13501 gatctagtaa aagaaatgca gcaaccagga cagttcttgc atattctcgt gccgcgtgca
    13561 gacttgctat tacgccgacc gattagcttg aatcatatcg accacgaaac aaatacttgc
    13621 cgtatcattt atcgggtgga aggtgagggg actagagtct tctctactct ttctgccgga
    13681 gaccatttag atgtcatggg tcctttaggc aatggttttg atttgtccaa tcttcataca
    13741 ggagacgaag tatttataat cggtggagga atcggtgtcc ctcctctata tgagttatca
    13801 aaacagttag tcgaaaaagg aattaagcca acgcattttt taggttttgc cacacatgaa
    13861 gtcgtttatt atgaaaaaga atttaatcgt ctaggagaaa caagaatcgc tactgacgat
    13921 ggaacatacg gtatgcacgg aaatgtcggt aatttattgt taggaacgaa gacggaacct
    13981 acagctgttt ttgcatgtgg ttcaaacggg ttattgaaga cagtggaaca gctgttttct
    14041 tcgcatcaaa atgtccagtt atcattagaa tcccgcatgg catgtgggat aggggcttgc
    14101 tatgcttgcg tttgtcatac gccggaagat gaaacaggaa caaaaagtgt gaaagtttgt
    14161 gacgaaggac cgatttttaa aataggagag gtaattatat gaatgagaat cctttagcgg
    14221 tcaaacttcc tggcctagaa ttaaaaaatc ctatcatgcc ggcaagcggc tgtttcggtt
    14281 ttggcaaaga atacggaaaa tactatgatt tgaatcagct tggatcgatc atggtcaagg
    14341 caacgactcc aaatgcccgc tttggcaatc ccacgccaag agtggcggaa acaccatctg
    14401 gcatgttgaa tgcaattggt ttgcaaaatc ctggtctaga cgttgttatg cataaacttc
    14461 ttccagaatt agaggagtac aatgagttac ccatcatcgc aaatgttgct ggtgcttgtg
    14521 aagaagacta tgtagaagtt tgtagcaaaa ttggcgaagc accaaatgtg aaagcaatcg
    14581 agctgaacat ttcttgccca aatgtcaaac atggcggaat tgcttttgga acagattctg
    14641 atgttgcttt tcaattaaca caagcagtaa agaaagtttc ttctgttcct gtctatgtca
    14701 aattgtcccc aaatgtaaca gacatcgttc caatcgctca ggcaatcgaa gctggagggg
    14761 cagatgggtt tacgatgatc aatactcttc ttggtatgcg gattgacctt aagacaagaa
    14821 aaccgatttt agccaatcaa acaggtggac tctccggacc agcaatcaag cctgtcgcta
    14881 ttcgtttgat tcaccaagta gcaagtatat ccagccttcc aatcattggg atgggcggcg
    14941 tgcagacagt ggatgatgtt cttgagatgt ttatggctgg agcaagtgct gtagctatag
    15001 ggaccgcgaa tttcaccgat ccgtatatct gtcctaaact aattaaagag ctccctgtgc
    15061 gaatgagcga actaggcatc gaatcgctgg aaagattaat caaagaagtg agagaggaac
    15121 gtgaacgatg aatcaactag accaacgacc aattattgca ttagattttt caactaggca
    15181 agaggtagaa gactttcttc gatttttccc gaaagaagaa aaattgtttg tgaaaatcgg
    15241 aatggaattg ttctaccaag aaggacctga aatcgtacgt tatctaaaag aggctggtca
    15301 taatgtattt ttagatctga aacttcacga tattccgaat acggtcgaaa aagcaatgcg
    15361 gggattggca aagcttggtg ttgatctcac ctgtgttcat gccgctggcg gtattcgtat
    15421 gatggaggcg gcaatgcgag gattagaaga ggggactcca gaaggtggaa aacgtccgct
    15481 acttctagct atcactcagc ttacttctac aagtgaagaa gaaatgcatg ccgatcaact
    15541 aatcgaagtt tctttagaaa aaagtgtgat ccattatgct agctgtgcca aaaaagcagg
    15601 attagatggt gtggtttctt ctgcttggga agtagaagcg atcaaagaaa tggcaggaga
    15661 cgaatttgtt tgcttaactc cgggtataag accagaagga acagtcgcag gagatcagac
    15721 aagagtagtc acaccttctc aggcaaaaaa gatcggctca acttttatcg ttataggtcg
    15781 accaatcact cagtcaacta atccttataa agcatatcaa acgattcaaa cagaatggag
    15841 ctaacctaaa atgaatgttg aacaaagtat cgcaaaagac ttattagaaa ttgaagcagt
    15901 atttttaaat ccaagtaatc cttttacatg ggcaagtgga atcaaaagcc caatttactg
    15961 tgataaccga atcacgatga gctatccaaa agtccgaaaa gagatcgcaa aaggattagc
    16021 aagcaaaatc aaagaagctt accctgaagt acaagtgatt gctggaactg caacagcagg
    16081 aatccctcat gccgcatggg tagcggaaat actggatttg ccaatggtct atattcgaag
    16141 taaggcgaaa gatcatggga aaggtaacca aatcgaagga cgtatatttg aaggtcagaa
    16201 gatggttgtg atcgaggatt tgatttctac aggcggaagt gtcttggaag cagctgaagc
    16261 agcaaaaaga gaaggtgcgg atatccttgg tgttgcggct atctttacgt atgagttgcc
    16321 aaaaggaaaa gcaaatttcg aaaaagccga aatccctctt atgacgttga ccaattattc
    16381 tgtattgatt gaaacagctt tagaagatcg ttatatcgat gagcaagaat taactctgct
    16441 aaaagaatgg aaaaaagatc ctgaaaattg gcagatttaa gcgcgcttga tgaagataga
    16501 gaaatccgat ttgtgtctcc agaatcatca attggaatag caaaaatcca aaaactgatt
    16561 gcgagaagca tcttttcatc aggtatgaaa agaaacttct atcgtgatca gtttttgatt
    16621 tttttaaaat tcatttttct aagactggtt agcagaaaga agagaataat ctcttctctt
    16681 ttttctatta tatatagatt ttctctattt tttggtaagg gagctgtttc aaaaatggta
    16741 aagagcggaa atgttgcgaa tatcagttat ttaatgatat gatattcgga aaaagaaatc
    16801 tcaaagggag gtaactttta tggggttgaa tgaatttatt ttaggattga acaatttaga
    16861 gacgatcaca agggcacctg gattttttaa atatacagag catacagttg cagcacattc
    16921 gtatcgtgta gcatctattg cgcaagttct aggagacatt gaagaagcga atggacaacc
    16981 gatcgattgg aaactgctct atgaaaaatc attgaatcat gactacacag agcgttttat
    17041 tggtgatatc aaaacgccgg tgaaatacgc aagcaaagag ttaagaggga tgctgcaaac
    17101 agttgaagaa aagatgacgg acgaattcat caaccaagag attccagctg agttccaaga
    17161 agtttatcgc cgtcggttgt tcgaaggaaa agatgaaacc gtagaaggag aactattagc
    17221 catttcagat aaggttgacc ttctttatga atcttttgat gaaatcatca aaaataaccc
    17281 ggaaaaagtt tataaggaga tgttcattga ggcagtgact acgattaaaa gctttgaaca
    17341 tcggccttct gttcagtatt tctttaaaga gatatttccg gaactattga accaggaatt
    17401 ttatggaaaa gaggcgtttt tagaaaccgt ctcaacgatt gtaaagaaag aagaaaaata
    17461 aaaatgatat gagatgcagg gggaagtata gttgaaaaag aatatggatg tcgaatgggg
    17521 ctattctgga gacaaaggtc cggaaaattg ggcgagttta tgtgattggt ttgcgagagg
    17581 tgcggaattt ccattgcagt cgcctattca tttgaccaag ggggaagaaa ccaaaataga
    17641 aggagacacg ctttcctttc attatcaaaa gcaacggttt actgacaagg aattcaaaaa
    17701 cacgattcat ttagtacctt ttgaccaact cagttatgta gagttcaaga aagaacgcta
    17761 ttatttgaca gatattcatt tccacttgcc ttctgaacat atcatcaacg gaaaacagga
    17821 agatattgaa tttcattttg ttcatatgaa cagcaaaaaa gagaatctag ttgtgggtgt
    17881 catgtaccag ctaatggtac aggaaggttg gctgtataat caagaaaatg gggaaagttg
    17941 gaacttagaa aaacatgagc attgggtaaa tccggatgtg ttctttccgg agcaaaaaag
    18001 ccatttccat tatattggct cattgacaac tccgcctacc agcgggccaa tccaatggtt
    18061 tgtaatggat catgtaggaa aactgaacca agagtttctt ctccccttcg atggtcagta
    18121 tgctcgaccg aacaaccgac caatccagcc cttgaatgga agagaaattt attatttcga
    18181 ggagtttgag caatgaatat tcagcagatg aaatacgtgg tcgctgtagc aaacaatggt
    18241 agtttcagag aagcggcaaa gaaaatgttt gtctcccagc caagtctttc agctggtatc
    18301 aaagaattag aaaccgagtt agggatcacg ctgtttactc gaacgaatcg aggggcatat
    18361 ttaacagaag aagggcaaga cttcttaaat cgtgccgaga aaatattggt ccagcttgat
    18421 acattagaaa ctcactatct aacgagtgaa aagagagaca atttttcgat cgctgcccag
    18481 cattatgatt tcttaggtcc tttgaccgca cagctactcg aggaattcaa agacgagatc
    18541 aaacaatttc ggatcatgga gacgacaaca gcgagagtga tcgaagaagt caaagagcag
    18601 cacagtgaga tcggcattat ttatatgaat gagcataatc aagctggtat cagaagatat
    18661 ttacaacaag gagagctaag tggccgcagc attggaaaat tcaaaacgca tattttttta
    18721 aggcaaggac atccattagc tgaacacgca gagatcacca aagaagaatt gttatcttat
    18781 cctcaagttc gttttacaca ggatggaaat aatttcccgt atttttatga agaccttatc
    18841 gagatccctg atcaagaaac agtgatttat acgagtgatc gaggtacttt gatgaatatc
    18901 gttcttgaaa ctgatgctta cgcttcagga tctggtattg tgattggagg gattcgcgag
    18961 cacttgcggt taatcccttt ggcagaaggc gaactaaatg aattttatat catttattcg
    19021 gcgaaacgtc cgctcagtga aatcgctcaa agatttatca aagagcttac tcggttactg
    19081 gaccagcaga gctaagaaaa gcagatgaga acgctgttta ttgaacaccg ttgatcaaaa
    19141 aagaggggtt gtgacagaat tttgtcacaa ctcctctttt tgtagcaaac aatttataac
    19201 taattgctgt tgtgggaaag tacgcaaata tgattctaga aacactcatt gattacctgt
    19261 tttgtttcat agctgtaatt ttttcttctt ctggtgtcac gataatcgtc ttttggtttt
    19321 cataaataac ataaccaggc ttggcgccat ttggttttcg gatatgcttt acctgtacaa
    19381 gatcaaccgg cacttgggca gagtatcggt attttgaaaa ataggcagct aactcggcag
    19441 cttctgtaat tgtttcatct gagggcttgt cacttttaat gatgacgtgc gaaccgggta
    19501 tattttttgc atgaagccaa tagtcggttt ttttagctgt cttcattgtc agctggtcat
    19561 tttgaagatt gtttttacca acaagaatga gcgttccgtc agaagaaaag tattgatcag
    19621 gctgagatgg tttcttacgt ttttgttttt ttaagttctt ctttttcagg tatccttctg
    19681 aagtcaattc ttctttaatt gcttcgatat ccattgggcc ggcaatttcc agttgagata
    19741 agacagattc aagatattgg atttcatctt tggcttcttg gatttgttcg ccaatcagct
    19801 taacagcgtt cttcagcttt tggtatcgat ggaaatattt ctgtgcattt tgatttggtg
    19861 tcaatgccgg atctagtgct attttgattg gccgatcttc ttcataataa ttaggtaaaa
    19921 caacttcttt tgcgccgcgt ggtacttgtg tcagaaaagt agtaagtagt tcgccatttc
    19981 tacggtaatt ctcagcgttt tcggattctt tgagtgtttg ttcacgtttt ttcaatttat
    20041 ttttatttcg ttttagttca ttttcgattt ttcgaatcag ttctccgcct tgttgttttg
    20101 cccgatcttt ctctgctttt tcgtgataat agctatctaa taattgactg agtgttggat
    20161 aagctgttac agcagatgcc tgttcggaca atacctgata agcaatcggt gtgaaaaaat
    20221 ctttattttc cgtttcgtaa aaagtaggaa taggctgatg gctgatggct gagaaaaatt
    20281 cgtcccatac aaccatcttt tcattaggac gttccagtaa tcttttggtc agctcttgag
    20341 cgatatcgaa accgattccc tgaaactgac gttggattgc ttttggatct agttcagttt
    20401 gggaaagccg ctgaaagatt ttttctttct cggagctaaa tgggttcaag acctcttgtt
    20461 ttggcggagc aacatattct acaccaggaa gcaaagaacg ataagtgttt tgagagcttc
    20521 caatgtgttt gatcgcatcc agtatttttc cagtttctcg attaactaag acgatcgtac
    20581 tatgacgtcc cattaattca acgatcaata tgatattttg tagatcaccc aactcgtttc
    20641 gtttagcaaa gtggaaatgg atcactcgat cattttcgat ttgttcgatg tcttctaaaa
    20701 tagcgccatc caaatatttt cgaagcatca tcacgaagtt aggcggatta tcaggattct
    20761 gataatcgat ttgggtaatc tgtacacgag cataacttgg gtgcgcagat aataataatc
    20821 gatggttttt gccgcgtgaa cgaatcacca gcacgatttc attttcataa ggctgatgga
    20881 ttttcgagat ccggccagat agtaacgttt cacgcaattc gttgaccatc gcgtgggtaa
    20941 atacaccatc aaaggacatg gttcattcct catttctttt cataagtata ttcattataa
    21001 cgaaaaaaag aagaaaaacc aatttggccg aatagtaaag caaaagtcta gaaaaatctt
    21061 ggactaatta cataaaaaac agtgtttcta tcgttgaaat ccaaattttt aggtataata
    21121 aaaaatagga gaaaaagtag aaaagaaggt gtttgttatt tgggaagtag ttaaatcgag
    21181 agtacaagaa atgaatgaac atttctcagt gtcattcgca gaaaatccaa gtaagtattt
    21241 tcgacgtctt gtttttctgc tcatcttatc attgctgtta acagttgcaa tgtttttatt
    21301 gccggaaaaa atccgagaag agcaagtaaa tagtctcgtt tattcgtcca taccaaccag
    21361 caaattgaaa ccttttgatt acagcaaagc tgatcagaaa atcaaacaaa gccgggcgat
    21421 atcggtgatg ttcagcaaac cgaacgggaa aacctatcag gacatgcttg atgtttttaa
    21481 tgatcctgag cttatggaag aattgaatcg accgatcttt tattatccga tcgtttatga
    21541 tgtacatgaa ctggaacaaa aatataacat acgaacggat gaagtgacat ttattttctt
    21601 tgatggagga aaagaagcaa atcggataac tgcgggtaag ggaggaataa ctgattttga
    21661 taaggagttg atccctgaac tgaatcgact tccgttagcc aatatcaaac aattagaaga
    21721 agaattggat agaacgggag taacttccca aaattccgag attgctaatt gagacattgg
    21781 cgcgatgaaa cggtgggtct atatagcgct atttttaatt atcagaacaa gtgatgtttg
    21841 atcttcaaaa taacaaataa aaaattaaaa aatatagttg acgaaattca aaaattcgat
    21901 tagacttaaa acaattctta aggaaagggt gacctgtaat gaacttatca aaacgtattt
    21961 cagcagaaaa caaaaaaaat tggcgcagct ggcgtagata agttgtatta atggtgagac
    22021 tttcccatag atacaacttt ttagagtgca tttctaaagc ttgtatctat gctagacatg
    22081 aacattgact gccgcataga ttagaatcta tgcggttttt tgtgtaaaga caatgaccgc
    22141 tttggccatt gtcttttttt gtagactgga tacttgcctg attataataa ataaaaagat
    22201 tgactttgca ggaggaaaaa aatgaaaaac aaacgattaa taacagttgt agtaattatt
    22261 ttagtttatt tagcaggtgc atttatttat ggaaaagcct caggcgaaaa agaaagtact
    22321 tcgacgaacc agacaaaaaa aacaacagtt gttggggtat tgcaatacgt gagccatccg
    22381 gcattagatg agatttatcg aggaatcaaa gatggtctga aagaagaagg attaaaagaa
    22441 ggagaaaatc ttgagatcaa atttcaaaat ggacaagccg atcagagcaa gctagccaca
    22501 atgagtcaac agctagtaca atctgatcta gatgccttga ttggaatcgc aacacctgcc
    22561 gctcaatcat tagcaaacac gacttcatcg atcccagttg tgttaggtgc tgtaacggat
    22621 cctgtggggg ctggattagt cgcttcttta gagaaacctg gtggtaatgt aacaggtgtt
    22681 tctgatcagc cgcctgttac ttcacaaatc aagcttgctt ctgatttgtt gccaaatgca
    22741 aaaaaagtcg gaatcttgta tgcttcttca gaggataatt caaaatacca agtagcacaa
    22801 gcagaagaag ctgcaaaaaa agaagggtta gaatcagtaa aatatgcggt gccttccact
    22861 aatgagattg cacaaacggt tcaagtgatg agccgacaag tcgattttat ctatgttcca
    22921 ttagataata ccattgccaa tgcgatgcaa acagtggtaa aagaagcaaa caaagcaaat
    22981 atccccgtca ttccatctgt ggatacaatg gtggaacaag gtggactagc aacaatcggg
    23041 atcaatcaat atcaattagg gcttcaaagc ggtaagatgg cggcagaact tgcgagtgga
    23101 aaagaaaaac cggaaacgac gcctgtttat acatttgatc aaggggatac agtgatcaat
    23161 caatctcaag ctgatcgctt aggaatcatt gttccccaat cgatgaagga aaaagccaaa
    23221 atcattacag atgactcgca gcaagaaaca tctaaggagg atgacaaatg atcgtatcag
    23281 ctatttcaca aggcatgctt tgggcaatat taggattagg gattttcatg acttatcgga
    23341 tcttagactt tcctgatatg acgacagaag ggtccttccc attaggtgct gctgtctgtg
    23401 tgacagctat tactcatggg gtagcgccga ttgtagctac attattaggc gtaggtgctg
    23461 gcatgatagc aggactcgtt acggggttac tttatacaaa aggaaagata ccggttattt
    23521 tagcagggat tttagtgatg tcaggattga actcagttat tttgtttgtg atgcgaacac
    23581 caaatctttc tttattgaat catccgattc tacaagacag cttccatcgc tttgggttac
    23641 ctgattactt tgacacagtc cttcttggct tcatctcttt gggcatcttg attgctgtac
    23701 tgctattctt cttcaataca gatttgggtc aaggctatat tgcgacagga gacaacgaaa
    23761 cgatggctcg gtcgttaggg ataaaaaccg atcgtatgaa aattttaggt ttaactttgt
    23821 cgaacggtgt cattgcatta tccggtgcgc tgattgccca gaacgacgga tatgcagatg
    23881 taaataaagg gattggcgta atcgtcatcg gtcttgcttc catcatcatc ggtgaagtga
    23941 tcttccaaga attgacgcta gcagaaagat tgattgctat tgttgtcggc agtatcattt
    24001 atcagctatt gattttagta gtaatcaaac tagggtttga cacgacttat ctgaagttgt
    24061 tctccgctat catcttagct atttgtctga tgatcccgca attgaagaaa gcactgaact
    24121 taaaaacggg ttttgagaag gaggcatagt aatgactgat atattgacaa tcaaaaatgg
    24181 cagaaaagtc gtaaacaatg gaatgaatga agagaaagta ctattggatg aaattaacct
    24241 tacaatcaga gaaggggact tcatcactgt ccttggcggg aatggtgctg gaaaaagcac
    24301 actgttcaat acgatcgctg gtacgcttca actcagtcaa ggatcgatct cttttaaaga
    24361 tcagctgatc acaaaagatt cagaagaaaa aagagcacga ttcctttctc gagttttcca
    24421 agaccccaag atgggaacgg ctcctcggat gacagtagca gaaaatttgc tacttgcaca
    24481 aaaaagaggt caaaaacgga aattccgttt acgtaacttg aaaaatcaaa aagaagattt
    24541 tttcagacta tgtcaagaag tcggtaatgg cctagagcaa cacttggata ctccagcggg
    24601 tgaattatct ggtggtcaac gccaagcatt aagcttgctg atggcgacga tcacagctcc
    24661 tgagttacta ttattagatg aacatactgc agctttagat cctaaaacag ctaaagcatt
    24721 aatgcgatta acaaatcagc ggatcaatga acaaaaattg acttgtctca tgatcacgca
    24781 tcgaatggaa gacgccttga aatacggaaa tcgtctgatc gtactccaaa aagggaaaat
    24841 cgttcaagat atttctgaaa aagaaaagca acatttgaca atgggcgacc ttttgcagtt
    24901 ctttgaagaa gtagtggatt gatttcgaca gaaaaattcc tttcagtaga aggacttgaa
    24961 aactcattga catcttcctt tgattcatcc tatacttaaa acaataaaca attaaacata
    25021 tttgggtgtt ttgtaggatc acgtagtcaa tgattttaac aattaaatag aatattcttt
    25081 gctctgacaa gtaaacgatg agaaaagaat ttgctacttt gccacctaga cgtttcgtac
    25141 tttttcgtat caaacgctaa gtggcttttt ttattgaagt aagaacaaag gaggaataag
    25201 atgatttctg gacaaaccaa attagctggg ctctttgctg atccggcttc tcacagttta
    25261 tccccattga tgcacaatac tgcttttgaa gcaaacaaca tcgatgcggt gtatttagct
    25321 ttccaagtaa atccaatgaa tttgaagcaa gcagtcgaaa gtattcgaac ttttgatatg
    25381 ttaggtgtaa atctttcgat gccgaataaa acagcggtta ttccttatct ggatgaggta
    25441 agccaggaag cgcaattgat cggagcagtg aatacgatcg tgcacgaaaa gggtcgtttg
    25501 attggctata atactgatgg tgcgggcttc atgcaatccg tgcaagaagc tgggatttcg
    25561 atcaaaaaga aaaaaatgac tctactggga gctggaggtg cagcaaaggc agtcactgtt
    25621 caagcagcgt tagacggcgc tgaggaaatc gtagtttata aacgaaacaa ccaaacattc
    25681 gctcaagtaa aagaaacatt cgaacaactc agctatcgaa cttcttgtaa gataaaagtt
    25741 cgtgactttg ccgatacgca tcaattaaga aaagacttga aagaaagctg tcttctagtc
    25801 aatgcaacag atatcgggat gggaaagaaa atcaatcagt caccgctctt agatataagc
    25861 atgatgcatg agaatctagc ggtttttgat ttgatttatg cacctagaga aacacgttta
    25921 ttaaaagaag caaaaaaagt aggagcaaaa ggatacaatg gacttggcat gttgatctac
    25981 caaggagcaa tcgcgtttga attatggaca aaggaaaaga tgccgatcca tgtgattgaa
    26041 aagctgtttg attaaaaaag gataatagat aaagatcttt tattctcgca taaacggtct
    26101 caagaaaagt aatcctttat gtatcgaagc atttatttcg ttatttttta ttaaattatc
    26161 aaaaaatgat aggcttttgt cgattggtta tcgtatcctt tatttatcag taagaaacga
    26221 ggaggaacca caatgagtcg gatggatcgc tataaggaca tccataagaa ggcgaaacct
    26281 ctgaagcggg atacggggag tatttttgct agaagagaac gaaaacgaac gcgtatggaa
    26341 aatgaggaag atgctacccg tatttatgaa agagagcagc attcatttga acatttgaac
    26401 caatacgagc aacagatata cagagatgca ccaagagcag aagaacaaca aaaaaataaa
    26461 aaatttttca atcggcaaaa gaagaacaaa ccgttaaaac ctcgaagaaa gcgatcgtgg
    26521 aaaaaaatca ttctatcgat tttgcttgtt ttgatccttt attcaggtat ctcatttttt
    26581 attggtaaat atgtagctga acatgacaca agcttagagg cagcgacttc agaaaccttt
    26641 catgggcaaa aagcgacgaa tggtgcgcat aatattttga tccttggaag tgatacacgc
    26701 ggagaagatg ccgggcgagc cgatacgatc atggtcttac agttggatgg tccttcccat
    26761 aagccgaaac tgatttcttt tatgcgtgac agcttcgtta cgatcccagg ggtaggacaa
    26821 aataagatca attcagctta tgcatacgga ggagccgatc tagtgcgtca aactttagta
    26881 gaaaattttg gaatcgattg tcaatactat gcgaaagtcg atttcaaatc gtttgaaaaa
    26941 gtgatcgatg ccttattcat gaacggagtg aagatcgatg cagaaaaaga tctgaatctg
    27001 gatggtgtag atattaaaaa agggggccag aagatggatg gacatgttct tttgcagtac
    27061 gcacgtttcc gaatggatga acaaggagac tttggccgtg tcagacgcca acagcaagta
    27121 atgaacgcta ttttcagcca attgaagaat ccattgaatc tgatccgtgc accttatgca
    27181 gctggtaaag ctttaggata tacatccaca gatgtcactg cttggttctt gacgaaaaac
    27241 ctcttttcga ttgcacgagg agctggtggc attgatcgaa taagtgtgcc agtagacggt
    27301 tcgtggaatt atgggcaaag tgattatgct ggcagtattc ttgaaattga taatcaaatg
    27361 aatcaatctg ctattacaga ttttttgaat aaatgatcat aaaaaagatc aagaagaagc
    27421 tgcgcaaggc ttttgtcatg aatcaatcaa acgattcact cgtaaaaaac gctttcttat
    27481 ggtagattta accaagagag gagctgttta cattggatat ctatctgaac ttcaaaaatg
    27541 agtcgcgtga agccatcgct ttttatgaaa aagtttttca gactgaagca tcaggtatca
    27601 ccacatacgg tgaaatgcct ggaatagaac aaatgaactt gccagaagaa gcaaaaaatc
    27661 taatcacgaa tgcacggctg aacatttatg atacattagt tatgtttgca gatacgccag
    27721 aaggaattgg tcctgaatta gttgtgggaa ataatgtttc tttagtcatc cagacaagaa
    27781 atgaagaaga aacgaagcgc ctatttgaaa gcttagctga gggtggtaag attgagatgc
    27841 cactggatgc tgttccgtgg gctcagttgt atgggatggt aactgatcga tttggcatca
    27901 actggcagat taaccaagag tgacagctaa agatgaatat tttctggaaa catcaagcag
    27961 caaacggatc atcgtttgtt tgcttgatgt ttttgtgtct atttcgacta tacggtatct
    28021 tttttttatt gccagatgcg aaaatatgtt ccataataga gaagtccgca aaaaattaag
    28081 taacggaggt cagaagatga agacggtgat actagctgaa aaaccttcac aagcaaaagc
    28141 ctatgccgaa accttccaac agcaaaagcg gcatgaaggg tattatgaaa tcagcgatcc
    28201 attgtttgct ggtgaagtaa ccatcactta tggttttgga catttggtag atatggttgc
    28261 tccaggagct tatgaagagc gttgggcaaa atggtcttta gaaaatttgc ctattttccc
    28321 agaaaccttc cgttacgaag ttccaatgga caaacgtgca caattttcga tcgtaaaacg
    28381 agaattgcag tcagccgata ccatcattat tgcaacagat ggtgatcgag aaggtgaagc
    28441 catcgcgtgg tcgattattc gacaagcaaa cgcattttct aaggaaaaga actatttacg
    28501 tttatggatc aattctcttg aaaaagaagc aatctatgaa ggttttaaga atctccaacc
    28561 tggaaagaac tattttccaa aatataaaga agcgcaagca aggcagaacg ctgattggct
    28621 gattggaatg aatggaagtc cattatatag cttgctactt caacgaaaag ggattgatgg
    28681 cagtttttct cttggtcgtg tacaaacacc gacactctat atgatttatc agctgcaaga
    28741 agaaatcaaa cacttcaaaa aagaaacgta ctttgaagga gaaggtttaa ttacgacaaa
    28801 caaagggcag ttttctggaa aaatcgtacc aaaaaaagca tttaaaaccc aagaagagtt
    28861 aacgaaggaa atagaaaaac ttggagcgca tttaggtaag caaaacggac ggattcttga
    28921 agttacgaaa aaggaaaaga ggttgcatag tccacgttta ttttcgctat ctagtttgca
    28981 gacaaagatg aatcagctaa tgaaagcaag tgccaaagct acacttgatg ctgctcaagg
    29041 attgtacgaa agcaagtttc taagctatcc aagaacggat tccttttaca ttacagaaaa
    29101 tgagcatcag tatctggtta aacatctgca aaattataaa aaatttttag gaatagaaga
    29161 agtacagaca actgagatca aagcaaagaa acgatatgtc gatgcgaaaa aagttcaaga
    29221 acatcatgca attattccga caaagacgat tccgactcct gcacgctttg cgaagctttc
    29281 caaattacag caagcaatct atttgcaagt tttgcgcact actgtggcta tgtttgctgc
    29341 agattatatc tacgaagaga cgaccgtaat gacgggcgta cagcaattaa agctaaaaag
    29401 catcggaaag atcccattac agcaaggttg gcaggaaatc ttacgcatca agtcaaaaaa
    29461 gaaagaagct cagactcttc ctcctatcgt agaaaatgaa gaagcagaag tagaactgaa
    29521 aagtatggag aaagaaacac aacctccaaa accccatacc gaaggaacgt tgatcacagc
    29581 aatgaaaact gcgggaaaaa cgctggataa tgatgaagcg caagaaattc tcaaagaagt
    29641 agaaggaata gggacagaag ctacacgagc caatatcatt gaaaatctta agcagaagca
    29701 ttacatcgaa gtcaataaaa acgaaataac tgttacgcca aaaggaatca ctctttgtaa
    29761 ggcagtagcc aatgaaccat tactgacgag cgcagaaatg actgcgcgct gggaagggta
    29821 cttgagaaaa attggtaaac aagaggggac accatctgtt tttttagaga atatcaaaaa
    29881 attcatccta catttattgg cagaagttcc taatcaaatc gacaaggtag acttttcaga
    29941 tgagattagc gggacacgtc agataaaagc gatggagaag aaaaacgatc aattaggaag
    30001 atgtccaaaa tgtaaaaagg gaatagtcat gctttatcct aaaatcgcta catgcttgaa
    30061 tccggaatgc gacttcaaat tgtggccaac agtagctaaa aagaagctga ctaaaacgaa
    30121 tctcagagat ttgctttcaa aaggaaagac aagtaaagtt gtcaaaggtt ttactggtaa
    30181 aaaagggaaa ttcgatgcag ttttagtatt aaaagaagat atgacaatcg gtttttcttt
    30241 tccatggatc gaaaacagtg gaacaaaagc agttgatgga aacaaagaaa catcaagaaa
    30301 aaataatgaa aacgaagtag agtaaatgaa aacaatgaaa tagcggaata tttattccga
    30361 aaagtgagaa tataaatacc aataaaacta aaaaatatca gtgaatcaag caaaaacgtt
    30421 ggggtaggct gattttatat agatagcaag gagttttttt atttgaaaaa agtgcattca
    30481 tttttaaatt atcttatggg agcaatcacg attgtggctg tgtaccaagc atttctaaag
    30541 tatcctgatt acgggacgaa gttgttgtca ttgacgcaca atcaaacagc atattcacca
    30601 acgttgattt tatcgacttt gactgcagga gctaaaagcg ctacttattt ttttcataaa
    30661 acgacaagca tgctaaagcg aaagaaacag cgaaaacaat tgttggtggt ggtagcggct
    30721 atgatcatta tttattttct accagttatt acacaagtat ttcatcaata ttttggttaa
    30781 gaatacgaga gtttgtgaca aaagtcatgt cacaaacttt tttctttgtc attttttcgt
    30841 aagaaaaagc aaaataaaag tatatacatt gattaaaagt atatacttgg atataatgta
    30901 cttactatag aatgagaatg gtatagaggg tggcgaaata tgaaaaaaat gaaaaagtat
    30961 aaaattagaa aatctggcaa tagcgatgta acaactatcc caccagaagt aaaagaattc
    31021 atgggtgtgc agacaggtga cgcaatcagt tatgtttttc aatctgatgg ttctgtacga
    31081 atgatcaaag cacaagaaga acctgatatt gattcattag ttgattcgat catgaatcaa
    31141 tatgaagacg cgttaaaaga tttggttgat ttatgaggta tctttcgact aaagaaatta
    31201 tcaaaatcaa cgcgaaagtt attttacgtc actcgccagg cgaaatgatc ggtgtaaaag
    31261 atgcaaatgc attagatatg gccgtcaaac agccttcaca agcagtattc aatcaagaac
    31321 tctatcctga aatctatgaa aaagcggcga ttttagcaat taatttagct aaaaaacatc
    31381 cttttcataa tggcaataaa cgaactgctc ttgttgccat gttgttgttt cttcaattga
    31441 ataattgtcc agttgctttt tctcgtcaag aagctgttga ttttatcata atgattacca
    31501 ctagttcatt ggatttcgat tcactaaagt ccaagataac cagttattta cgacaaaccg
    31561 caataaaatg aacgaattaa aaactttatt tctcaattag atcagcatgt cattaaggaa
    31621 tatgctgatt tttttatgtt taatcataca aaatagtttt ttcataaaaa tatggaaaat
    31681 atggattaat tttgaaataa ttgatttaaa aggaatacaa aagagtgaat agtaggaaat
    31741 agtggtttag aactagtggg ctgatttttt atatatttta gtttacataa tatatattat
    31801 acaaagtaga tttaaaaaat gataaacctc tctcgaacgg ttttttatca aaaatcgtca
    31861 tttttccatc aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt
    31921 tttttgtcgt ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa
    31981 cgaattagat atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga
    32041 taaacattga taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg
    32101 tacggataca ttttcggacc gattaatttt aaccattctg tttatttgct tacctattag
    32161 atatcaataa aaaactgctt aacagctatt tcagctgtta agcagtttta ggatttttag
    32221 ttcatattca ttgtttctta taatacagtg ttcaatactt ctcttaaagc acttgttgat
    32281 ttgctgaaca ttgctttttc ttcttcgttg attgataatt caacaacttc acgaacacca
    32341 ttttcgtcaa cgattgatgg cacacctgta aagatatctt tttcgtcgta ttcaccagtt
    32401 aaataagcag atacaggcaa tacagcttgt tcgttattca agatagcttt tacgatacgt
    32461 gcagtactca taccaatacc atagtaagtt gcttttttac gatcaatgat ctcataagca
    32521 gcattacgga ctttatcagc aatcacatct aattcatctt gggcaatacg atggtctttt
    32581 tctacaattt caaacactgg tttaccaccg acagttgtat gtgaccatgc agcaacttct
    32641 gaatcaccat gttcacctaa gatatagccg tggacgctgc gtgggtcaac ttttaatttc
    32701 aatgcgattt ctttacggaa acgagttgta tccaatgttg ttccagtacc gataacacgt
    32761 gatgttggca gaccagattc gttccaagcg atatatgtca aaatatctac tgggtttgaa
    32821 gcaacaacga taataccgtc gaatcctgat cccatgattt cttttacgat ttggcgcatg
    32881 attgacgcat ttgttttaac taaatctaaa cgagattgac caggtttttg gttgatacca
    32941 gctgtgataa caacgatatc agcatcttta cattcttcat agccacctga ccatacagcc
    33001 acattttcat cgccccaagc cataccatct aaaaggtcca atgcttcccc ttctgctttt
    33061 tcttgattca catcgatcaa tactaattca ttagagattc cttggttgat cattgcataa
    33121 gcgatacttg ttccaacaaa acctgtaccg acgattacta ctttacgact tgtttttttc
    33181 atgaataaac agctcctttt cataactatt tgttactttc atgaaaagta tagtcttgtt
    33241 tattcatttt atcaacatgt taattataat agttgttatt ataatttttg atggaaacga
    33301 attcctgaat acgcataaca gataaatgac aaatgtttac acctatatcc cctgataata
    33361 caaccaaaag tttagaactg aaaataaatc caaaaaaatt cgctgcaacg atataataag
    33421 acagcaatcg cacctaaaac aattttcctt catatgcttt agtgaaagat tttcttctta
    33481 atgtttattt ctccattcat tgggagtaca tgttgaatac ttcttgaata atttgaataa
    33541 agaagtcgtt tgtttatatc cgatacgatt agcgatttct tccaacgaaa aactcgtttc
    33601 ttgaatcagt tccaacgcaa agtcgaattt tttctttaga accagttctt gaaaggaagc
    33661 atcaaaatac attttcaatt tattactaag atagaccgga ttgtagccaa aatgagctgc
    33721 tgtttctttt aacgtacaag aagtaaaatg cttatcgata taatctaata ctctaagcat
    33781 ctcattttct gcgcatccat ctgctgatga aaagctttgt tgttctaata aatgagtcaa
    33841 ttcgatcagt aatagtgatg tatagagatt taacgaacgg atcttatgac tgtatgtatc
    33901 aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg
    33961 aaaatgaatg aattgtgtat gttccccttt ttcttttgat gcatttatca aaaaagaaga
    34021 aagtagatta tttgtgcttg ccaaatgatt caatatctct gtagataagg tttcttcttt
    34081 taataagatg ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt gagtagcttc
    34141 tttatcaatg atcaccacgt cactttcttc catctctatc ctttctccat taataatttg
    34201 taaacatgta ccgctataca tataattgat ttcaacaaaa tcatgtgtat gctcgggaac
    34261 ttccgcatat cgacggtgtt tactgatatg aatatttccc tgttgaaata gatcccctcg
    34321 gtggattttt ggcggtgtct gaagatggta agctaagttt aaatcattga tattcattcc
    34381 ttgttcacgc tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg tctaattcag
    34441 ctagttccaa ttgactccct cctttttgta agcggtttct ttcgcttatt agttcacgct
    34501 gtactgctat tatatgcaaa gctatttttg gtttcataaa gactttctat ggttgggaca
    34561 tgaacttcct gtgattccgc tattgagaga ataaccactt catactatat ttttgataag
    34621 cgcttacaaa aatgaaagga acatgcttat gaataaaaag atgatgaaat tttctctgct
    34681 gtcagtctct cttattttga cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc
    34741 agctacttat gccacgcgtt ctttatctgc tgttgaatta ttaacgactg ctccctcttt
    34801 tatggtcact atttttgttt tgattagtag ttttattgct aggaaaatcg gacaaaaaca
    34861 aactgtgatc atcggattag tgattgcatc gatttatggt atcctccccc tcttctcaca
    34921 aagttacagt attgtcttag cttcaagagc tggactaggt attggctttg gattgatcaa
    34981 ttctttggca gtcagcatga ttgctgcctt ttatcacgga gatgaacgag cctctatgat
    35041 tggttttcaa agtgcatttc aaggatttgg tgcagcttta atgacatttg ttgcaggaca
    35101 gctagtacag ttcggttggc aatatacctt tttagtctac gccatcagct tacctattct
    35161 tgtcttgttt gttttgtttg ttcctacacc tgaaacaact gaacttcaat cacatggcac
    35221 agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg attagttatg gtatttttct
    35281 cttgctggtc atcgtggtat ataatgctgt atcgattaaa ttagccacag tcctgacgac
    35341 ttattctcta ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc aattggcaag
    35401 tatggctgcc ggagtttgtt ttggctggct tcagacaaaa gcacatcgct atatgcttac
    35461 actttcttta ctactgatgg gaatcggctt tgtattgatt gccactgtga taaacctgta
    35521 tgtgattggg tttggtgcga tcctaacagg aatttctttt tcgttattca ttccttactt
    35581 attcaaccaa gtaagtatcc aagcgcccaa gtacgcggag agtttcaata cttcaatctt
    35641 attggttggc gctaatttag gtactttcat tgcaccatat ggtttgctct ttctctcttt
    35701 tgcttctctt gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct tgctgttgat
    35761 tagcgcgctt attagccttc tagtagtaca caggacgaat agaaagacag tgataagagt
    35821 agatgaagaa ataaaagaac aattgacaac aaaataaatt tgatggaggt cgtgtaaaat
    35881 gacaaatgaa aaacataaag atagatggct ttggtatcct ggagactttg agatccgcca
    35941 tggactgcta caaaatttcc aacgggaaga acgaggtttt gactggccag cgtactggta
    36001 catggatgat tgccatcgga acgtcaagtt taagagatat tactttcttg atcaaccttc
    36061 catgtttaaa gtgacgatcc aaggagttgg gtatgtagaa attaatggac aaaaacatcc
    36121 atgtggaaaa tggctgactt gtccagctgg taaagcaaaa attcgtatct ttgtaggaca
    36181 tacatcaggg ctaccagcga tgtttatcga gggagacgaa gtaaaatcag atattggctg
    36241 gacagcaagt aattttattg aagaatatcc agctggctgg agtccgcttt atgtcgatat
    36301 cgcaaaagat ccaaatcaaa tctattatca aaaagaatgc gtccatccag tcactgaaac
    36361 ggagattggc ggaggaatct tattcgattt tgggcgagca gtgaatggct gtgtagaact
    36421 aatatctctg gatgaccata tcgaatctat cactctatgt tatggagagt ccgatgtaga
    36481 agctatggat atcgattatt gctactataa gcaaatagga atgacaagaa aaatgaaagc
    36541 tcgtaaacgc gcttttcgct atttgtttat accagaggtt tctactggaa tggttcaact
    36601 tcaagttttt catgaatttt tatctctttc gaaacaagct gctttttcaa gcgattcttc
    36661 tttactgaat cagatctggt cagtatcaga agaaaccttc aagttatgta gtgatttgtt
    36721 ttttatcgac ggtatcaaac gagatcgctg gatttggtct ggagatgcgt atcaatcatt
    36781 catgatcaat caatatcttt tttttgacga ggaaatcaat aaacgaacga tattagcatt
    36841 aagaggacaa aatgaaatca agcagcatat gaatactatc gttgactatt cgatcttgtg
    36901 gttgattggt atcgaaaacc actatatgat gacgaaagac tatgaatttt tacgcatcgt
    36961 ctaccctaag atgcaatcat taatgagata cttgatggag caaacgaatg aattagggtt
    37021 tatttatggg agagcaaaag attggatatt tattgattgg tctgaaatgg ataaagaagg
    37081 aacgcttgct gcagaacaag tcttattact gaaagcctat cattccatga tcacttgcgg
    37141 agatatctta gctgaaacat atggagagaa cgaaaattat catactcatc tctatcaaga
    37201 aaaatatcaa ctattgaaac aaaacctatt tgactacttt tgggatgaag aacttggcgc
    37261 ttttatcgat tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga atattttcgc
    37321 tgttttattc gattttgcta acaaagacca acagcaatcg atcttaaaga atgtcttatt
    37381 gaatgacact atcacacaga ttactactcc atatttcaaa ttctatgaac aagatgcttt
    37441 atgcaaactc ggacagacga atatcgtcta ccaaacgatt cttgattatt ggggagggat
    37501 gttatctcat gatgcggtaa ctttctggga agaatacgac cctactcaaa caggaaatga
    37561 aaagtacagt atgtatggag atccttatgg aaaaagctta tgccatgcat ggggagcaag
    37621 cccaatctat cttttaggac gctattttat cggtttgtat ccaacaaagc caggttatga
    37681 agaatttatg atacaaccta atctagattc atttgaaaca ctgaattgta cgctacctat
    37741 caaagatgga aagatttcgc ttgaaaaaac aaaagataag atcgtgatta cttctgatag
    37801 aacaggcgga acagtgaaaa tgaatggtaa aagttatcct cttctagcaa aacagccact
    37861 tgtcttaaca agctattcaa aaataaatgc ataaaacaaa aaactgcttg cgacatatca
    37921 gaagacttta aatcttcttc tgatattgtc caagcaattt ttattttgaa gtttgcttaa
    37981 ttctgttctt tagaaacaat gtttgttctt tttccggcaa aaagaaagct agataatacg
    38041 acaagtaaaa agaaagcgat gattccaact gtcaaggcta tttgaccgta ttctcctagt
    38101 ttcccaagta caagtcctgg tactagataa tcagtatcgg caaatgtaga agaagcgatt
    38161 ttaagatctc ctaatactgg aaggataaat aatggcagcc aactaatcaa taagctattg
    38221 aaagcagaac ctaataccgc ccctcttcta ccgccagtcg agttgccgaa gacaccagaa
    38281 gcagctccgc agaagaaatg tcctactacc ccaggaatga taactgtagt ttgcaagcct
    38341 atcatagcaa acatggatag tgttcctaca aagaagctaa caaaaaagcc aattaataca
    38401 gcattaggtg catatggaaa aacgatagga acatctaatg ctggtttaga atcgggtacg
    38461 agttttttag caatcccttg gaatgctgga acgatttctg ccaacaccat tcgaacccct
    38521 tgaaggacga taacaaaccc agctgtgaat gttccagctt ggatcagtgc aaagattaca
    38581 gcgttgtctc cattgcttag ttctctttca acaaatccag gaccagctaa caaagcaagt
    38641 gcgacatata cgatcgacat caagagcatg atcgatacag tagaatctcg caaaaagctg
    38701 aaacttttag gaatctttat gtcctctgta gaacggtctt tgtttccaaa aaactttccg
    38761 atccatccac tagaagcata accgatattt ccggtatgcc ctaacgcaac ttgattgttt
    38821 ccagtgatgt tcttcataaa cggttgacaa agagcaggcg taatagttaa tagtgttcct
    38881 tcgaagaccc cgcctaataa gatcatccac aaatttgcag taaaccccgc gcttactaaa
    38941 atgactgctg ttagacaaga aacataaagc attgcttgac ctgtaagaaa aatgaattta
    39001 tatcttgtga atctagcaag taaaatattc acgatcatcc ctactagcat gatcaaagca
    39061 gtaggtgtac cgtattgttg caaagcaatc gccactactg cttcattgct aggaactact
    39121 ccttgagtat gcagagctgt ttggaacatc gaagcaaaag gggttagtga attactgatt
    39181 aaaccagcac cgccgatcag tactaggaat ccgacaaatg ttttgatagt tccttggatc
    39241 acttctgaaa atttcttctt ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg
    39301 atggagggct gactgacgat tgacacaaac aaattcaaca atttatccat tgatagttcc
    39361 tcccttgaat catttttatt cttttgacaa gatcttcgtt actttttctt ttacttcttc
    39421 gctgtcaatg atcgattgca aaggtacgag cttttcatca ggtatagaag ttagcgtatc
    39481 tttcaaagaa tgctcaatga agaaataatc agccatatcg ctagatacac ttccaacatc
    39541 cgtatgactt acttcaaact cgtccacatc tgctccaaga tctgcaagtg cttgtttgat
    39601 gttcatctct accataaaac ttgttcctaa tccactttga catactgcaa aaattttcat
    39661 atcattcgtc tccttttctt atgatcgcta atatttcttc ttttgttgat gcgtgtaata
    39721 aatcttgaag attttgttta ttagaaagta gctttgttaa atttgccaaa gcccgaagat
    39781 gtgcctcatt atcgatagca gctaaacaga tgaacaaact aatgggatgg gctggattat
    39841 caagcagatc cacaggttgt tgaagtttca gcaaagacat ccctacttcg tttacgccat
    39901 cttctggacg agcatgaggt aatgcgatgt gatcgccaat atggataaac gcaccatact
    39961 gctccacgcg ctcgatcata gctttgatat aactttcttc tattttattt tctgataata
    40021 gcggctgtgc ggcaaatgtg attgcttctt gccagttttt tggttgatcc gataattgga
    40081 tcatttcttc tgttaataat tcagatagca taggacgttg atcctccttc ttttctaaaa
    40141 gtttgttcat tttacgattt aatacttgat agagcttttc ttcgtctacg ccttctttta
    40201 attcgatata aggcagtagc gccgacaaca attcttgggc agaaggcatt gaaaatcctg
    40261 gtatcagcca atctctttgt acttgattga tcagctgatt tttttccaag tcagtcatga
    40321 ttggatgcag aacatagaga gactttttct tatcagtaag tgtgagaggt acagtagaaa
    40381 aaataatatc gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg
    40441 taaaaaagat catcgggaat aattgctgaa gttcactttt taagatcaat gaagagctaa
    40501 tgccacttgg acagacgatc acggctctta tttttctatt tctttcttct gcgtccgctt
    40561 ttctgatctc tccgccaaat agtatagtga aaaaaccaat ctcttcttca gggatgctct
    40621 tccctattcg tctttctaac ggaagtaatg cttttcttgt catttcgtag atggaagcat
    40681 attgatgctt aatttgatcg atcatcacat taggtaaata aaaagaatag gaaatacgaa
    40741 aataggcagg gaccaagtga taaaagacat tcatcaataa ttcacgaaac gaagcaaatt
    40801 ctacggccat caggaattcc atgcgatgga tgacttctga agaacattcc agcaaaaaat
    40861 caagtgcagg ctcctggaca tctccttcaa cgatcgatgc taagcaaaga gagaaaaata
    40921 taatctccga attttgatgt tcggaatagc caaggccagt caatacttgt cgactaatgt
    40981 cagtgactgc aagagaagcc aacagttcag catcttttac cagttccatg gatgtttttc
    41041 caattcgaat ggttgtcaaa ctcaaataat aagctaattc atccattcga tttggtgcga
    41101 atcgaagatc tgatagtgaa atcgttttat ctagcaaatc tctaactttg gcgtaataat
    41161 caatggaaag ggttgcggtt aaatacgtga gcccaaatct tccagcaagt gactgcatca
    41221 aacgagcagt gcgatttttt gcttctcttc ttaatattgc ttctgaccct agcagcctaa
    41281 aaccagtttt cctagtatac gacagctgac aatcttccac ttctaattca tttcttatct
    41341 ttttgatatc tgacaggact gtccctttag aaacctgaag aaaatcttga aaatgaaaaa
    41401 cagaaacaga cgttgcagtc gaataaacca tcaaataaag aagatctctt cgctcttgtt
    41461 cggtatacac agcttcaaat cgagtaatat ccaaaaattt ttctgcccag tcgattgctc
    41521 ggaatttctt tatacggata cgggtatcat ccacatattc ggcaaaagga gttaattgtt
    41581 tgatcgcctc gcttgtattt acttcgatga tctctgctgc tttttctaga ctgatttctc
    41641 ttggtgtttt caggagataa ctaattttgt agttcacagc gctgttcacc tcctacttat
    41701 attctagtat ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac agttggattt
    41761 tttttacgac tgtaccgaac gatttctatc gaatggatac agaaaaaaag caggtgacca
    41821 aatgtctcct gcttttatga attgatttcg tccgatcaat gatgatcatg tttatttttt
    41881 gaggtcagtt cttcaaattt ttctttgaca tcttcaaccg ctttttcgat atctttttca
    41941 aagtgattgg gctcattagg ctttgtttcc tcttctacag aatttgaacc agataaagct
    42001 gtttctttat tttctgtttc accagagttt gtgccagcta atgattcttc tttgtcttct
    42061 gtttcaccag aattagttcc tgctaaagcc acttcttctt ctgttttagg ggccattgaa
    42121 cctcttaatc gtttgtgttt ttcaccgtca taatttgtca tgttggttcc tcctagtagt
    42181 tctatacttt ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa cccgattctt
    42241 acaaatatgt catatttttc cttctgattc ttgaatgctt ttgattcctt cttccaaaat
    42301 tttcatttgt aagattgttt cttcatcttt gatgactttt tctgcgccat taactaatgt
    42361 ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc tcggaaatta ctttttcttc
    42421 atgataactc ccttgttcat ctatataggt caatacacca taatgttcgg gcaaatcgat
    42481 accaaagtct tcatgatcag gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt
    42541 tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg aaacttggac gttctttgat
    42601 gcggaaataa ctggatttta tcgaaatttt catatttcca tagaaaaaat ccagatcgaa
    42661 gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga tatcatctgg
    42721 ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat ataaatagct
    42781 gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat aatcataatg
    42841 catttctact tctaaaatat cccctaattt tccgctttcg attacttttt ggactgtcaa
    42901 gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt tttcttttgc
    42961 taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct ctactagtac
    43021 attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac taggtgtagt
    43081 gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat cagtatattc
    43141 gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt tgatattgat
    43201 tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct ttccattgcc
    43261 aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc agtatatcct
    43321 agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag aaaaacactt
    43381 gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca aaaagatagt
    43441 agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg cttattgaag
    43501 aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc gattacatcc
    43561 gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa gctacgtata
    43621 cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga tttaaagaat
    43681 tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat caaacgattg
    43741 atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag aaaatggcgg
    43801 atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa gatttagatc
    43861 aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa ggtgattctc
    43921 aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt ttgatcatgg
    43981 ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag gattgcgcca
    44041 tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc tatttgaagc
    44101 atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg gccaaacagg
    44161 ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta gcgactttct
    44221 cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata tattctcaaa
    44281 attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa aaaggccttc
    44341 ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa agatagtgtt
    44401 tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt tcaaacctca
    44461 gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga atagctaact
    44521 tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga aaatcagtat
    44581 acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt tctgtacttc
    44641 tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta agcgatgact
    44701 gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt tttcgattct
    44761 cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat aagcgagtgt
    44821 ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta agtctgtgaa
    44881 caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat tttctccccc
    44941 cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca cagtattcgt
    45001 ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac cgatcgtatg
    45061 tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa agggatctgc
    45121 acctggattt ccaccttctt ttgataattg ctggtttttc tttgtcatgt tttcttggat
    45181 tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag agatgatctg
    45241 ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta tccctattcc
    45301 tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg gtttcatttg
    45361 tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc gtaatccgct
    45421 gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt ctagcaaagt
    45481 acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag attcccttac
    45541 cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga gaaatcaact
    45601 gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt gttaatggaa
    45661 catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt agacctgtca
    45721 catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc gccgataccc
    45781 agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca ctatcttgat
    45841 cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca ccatctactt
    45901 taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga tcatttgtat
    45961 gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct ttttcgttca
    46021 aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga atataattcg
    46081 gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt actgggtcga
    46141 tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca ttatcaaatg
    46201 ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt
    46261 ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt ttttcaccga
    46321 ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac gtatcatttt
    46381 tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc gtgattgttt
    46441 gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta tattttccaa
    46501 cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca aagtcattgt
    46561 tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga tcagttgtcc
    46621 aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt gttccttctt
    46681 ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta ccgatttttg
    46741 taactttcaa cgttccatca ttacagactg tatttttagg gtagagatgg atagtatcta
    46801 gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca tatacaggga
    46861 aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt
    46921 agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt ccatcagcat
    46981 ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct tgttttgctg
    47041 catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg ttataaactg
    47101 aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct ttcccgctgt
    47161 tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg acttgagctg
    47221 ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa
    47281 ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt tcactcctgt
    47341 tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa caacgcctaa
    47401 aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt cttttttatt
    47461 gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa
    47521 cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt ttaatcgata
    47581 acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag
    47641 cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga ggtaatttcc
    47701 tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt tatttgtctc
    47761 gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac tgacaccaaa
    47821 accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg tactgcttag
    47881 ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg aagggatcga
    47941 aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg tcagcttttc
    48001 tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat ccgcactatc
    48061 tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat gcaaggaata
    48121 agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct tctcaaaagg
    48181 tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg aaagtttttg
    48241 attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt ctttgacgac
    48301 ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt gatctttagc
    48361 ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg ttgcttctgc
    48421 ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt cttctaccgt
    48481 tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac
    48541 accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat ttggatgatt
    48601 tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt
    48661 ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga agagacacat
    48721 actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga
    48781 taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt
    48841 cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg
    48901 tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca
    48961 tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa
    49021 gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct
    49081 tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct
    49141 tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga
    49201 ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg
    49261 atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca
    49321 ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa actggcaaga
    49381 cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc
    49441 tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac
    49501 ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca
    49561 tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt
    49621 tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta
    49681 taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga
    49741 tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc aataagatcc
    49801 ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta
    49861 tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg
    49921 attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct
    49981 tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt
    50041 gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt
    50101 gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg
    50161 tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt
    50221 ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc
    50281 atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa
    50341 tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct
    50401 tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt
    50461 gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca
    50521 gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca
    50581 gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac
    50641 tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca
    50701 tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta
    50761 ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga
    50821 gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata attgctgctt
    50881 gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc cgtcagcaaa
    50941 acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct
    51001 ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttgatttttc
    51061 actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag
    51121 tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca
    51181 aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac
    51241 cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca
    51301 ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca
    51361 tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga
    51421 tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga
    51481 tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca
    51541 tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat
    51601 aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct
    51661 atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata
    51721 atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga
    51781 tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt
    51841 ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag
    51901 taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt
    51961 agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt
    52021 tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat
    52081 aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt
    52141 cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag
    52201 gcgttacata attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga
    52261 atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga
    52321 gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaatgc
    52381 aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact
    52441 aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa
    52501 gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat
    52561 cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt
    52621 tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat
    52681 tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat
    52741 caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc
    52801 cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga
    52861 agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat
    52921 aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg
    52981 tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga
    53041 gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc
    53101 ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt
    53161 ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg
    53221 tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa
    53281 ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg
    53341 atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga
    53401 tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta
    53461 tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat
    53521 aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat
    53581 atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat
    53641 cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg
    53701 aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa
    53761 ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat
    53821 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
    53881 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
    53941 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
    54001 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
    54061 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
    54121 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
    54181 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
    54241 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
    54301 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
    54361 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
    54421 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
    54481 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
    54541 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
    54601 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
    54661 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
    54721 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
    54781 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
    54841 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
    54901 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
    54961 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
    55021 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
    55081 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
    55141 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
    55201 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
    55261 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
    55321 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
    55381 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
    55441 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
    55501 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
    55561 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
    55621 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
    55681 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
    55741 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
    55801 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
    55861 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
    55921 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
    55981 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
    56041 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
    56101 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
    56161 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
    56221 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
    56281 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
    56341 taaagtgaca ttttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
    56401 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
    56461 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
    56521 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
    56581 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
    56641 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
    56701 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
    56761 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
    56821 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
    56881 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
    56941 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
    57001 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
    57061 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
    57121 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
    57181 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
    57241 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
    57301 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
    57361 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
    57421 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
    57481 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
    57541 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
    57601 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
    57661 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
    57721 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
    57781 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
    57841 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
    57901 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
    57961 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
    58021 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
    58081 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
    58141 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
    58201 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
    58261 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
    58321 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
    58381 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
    58441 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
    58501 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
    58561 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
    58621 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
    58681 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
    58741 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
    58801 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
    58861 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
    58921 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
    58981 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
    59041 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
    59101 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
    59161 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
    59221 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
    59281 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
    59341 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
    59401 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
    59461 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
    59521 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
    59581 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
    59641 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
    59701 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
    59761 aaacaattta attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat
    59821 gtcatttgtt tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat
    59881 ggtttaattt atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc
    59941 aatataattg ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt
    60001 tctaatactt ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa
    60061 tggagagcca atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc
    60121 ctcctgccat tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa
    60181 aaagaggata aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa
    60241 tgtatcttct tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat
    60301 caagacaaac tgtctatctt catttgatga aatgcggtaa aatatagaaa atgaacacta
    60361 ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca
    60421 acgtacacac ttgctccctt ccgtctttca acagttttta gaagaaaatc gttcttccct
    60481 ttcattaggc agtcgctatg aatacgcaaa agattttcat ctgtttactg attatttgag
    60541 aaaagtattt tctgaagaag tggatgatca gctgattatc catttagaaa aacaagatta
    60601 tcagaatttc ttagctaaaa tctatcgaca tactcgttcg ttccaaacta cagcgaacca
    60661 agaaacggag caattgtttg aaaatagcta ttacgggatt tcaaggaagc aatattcatt
    60721 aaatcatttt ttacgatttt tgtatcaaaa aggactgatt gaagaagaga tttctctttt
    60781 gggtacaagt ttaccggaaa caaccaaagc agctccgcga tatatcgatg cagaactttt
    60841 caaagatttt gagcatttgt ttatcccgat tgaaggcttt caaacaagca gagaagctag
    60901 ctttgaagaa aagaatagac cgaggaacct tgcaattact gccattcttc tgtattgcgg
    60961 attaaagatc catgaagtag tagaacttaa acgtaaagat gtatctttga ccaagcagat
    61021 tcttaatctg aatagaaaag aaaacagact gaataaagta ccaataccaa aagaagcgat
    61081 gccatttctg gagaactatt ttaagttaac agcacaaaat acggacgaaa attcttttgt
    61141 ttttcgttcc tcacatgatc agcaaatttc tcctcggact attcgccaaa ttttgagcaa
    61201 aattacagta tataaaaacg tatcgccaga actatgcaga aaaacatatc gttattatgc
    61261 agaactatat ttagatgatg ctgacgcggt aaattattta tgtggtaatc gatacctctc
    61321 atcccattct tttcaagata tctgggaaaa gatggttgat ttttcttatc acgaattagc
    61381 ggcattcgtt cgggtttctt ccatataaaa caaatgtatg atcttttttt cacaataaac
    61441 tatgttatta tataaaggat tatgttacga ttatcttgaa tttgtatatt acttaacaaa
    61501 agataatgga gtgtgaatga tggagaccat aacgtattcc cctttgaact tatttacgaa
    61561 ttttcaagaa gctgcagcta acttttccga gaccccgatt atctttgatg agccactttc
    61621 agctttccca gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa
    61681 acgggcttat caattggctc atcttggagt gaaatccgga gacaaagtta ttatttataa
    61741 gagttccgct tttgacacat accttctagc tgtttctgtg tcttaccttg gtgcagtgcc
    61801 tgtcatgact tcttatcatc taccaacaac aacaatggaa gtatttatcg atcgtttaga
    61861 agaccctttc attttatttg atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg
    61921 aacaaaatca aaacagattc ctattcttta tttaatggaa caacctgctt tgtctgtttc
    61981 tcaatcattt ttagacaaaa atgaaatttc ttatatgacc catacttctg gtacaactgg
    62041 tatccctaaa cttatttgtc attctgctca ttctatggga tggcggacga aatggcaaaa
    62101 aacagttttc acaaaaattt cggaaaagaa attaatcgct tttcacatct ctccagtgca
    62161 ttccagattc aatattggta tctcttcttt gatgtccatg ggatttccta tgatgccttt
    62221 agcaaacgca caaagttcaa aagttgttca tatgttggaa gctcaccggc caattgcact
    62281 agagacccat ccgaataact ttgttcagtg gcgttttact gctaaggagc atccagaagc
    62341 tttttcgggt atccgatact atcactccac ttttgatgct ataaacaacc agacaatgaa
    62401 gacatttttg cgaacgtcga tggcacagga tgcggtattt cttcaagtat atggccaaag
    62461 tgaatgcgga ccaatgattc ttaaggctca tacactagag agtttgaaaa caagtgatgc
    62521 aagagatatg ggcgttggac tggaagacat gacaagcgct cggattacag attcagttgg
    62581 gaatgtccta cctgaaaaca cagatggtca tatacaatta ctgtctaaag gacgggcttt
    62641 gacttattac aaggaagatg cacggttcca ggaaaatgtt tatggcgatt ggtgggatag
    62701 cggagattac ggttttatgg ataagcatgg ccacctattt ttgaaagatc ggcaagtgga
    62761 tttgatcgaa acgatcaata gtacattagc tattgaagac tttttattag attcgttaga
    62821 ttttttggca gaagtagtga ttgtaaggga taaaaatcat tctccacaac cgattattgc
    62881 tttagctcct gataaagaaa tggattggaa tcgatggtgg gagcaagtcc atgaacttcc
    62941 tcgcttgaac gtccctatca tccgtgattt tgatgcaatc ccgcgaacag ctacaatgaa
    63001 agtgcaacgg cttcaaatcg agaaagaatt aaaatcacaa aagtcctaac tatttaagga
    63061 aagaaagatc cattttggtc tttcttttct ttttgttttt ttaaattgga tgagataaaa
    63121 atatgccaat tggctgtttt tcttttttga aaatttcgtt atactcttat tcatggagga
    63181 aaatgttatg aaaagaatct tgacaattgg cggttctgat ccatttgctg gtggaggaat
    63241 ccaaacagat ttaaaaactt ttgagaattt tggtctattc ggactaagcg ccttgacttc
    63301 tataggggca ctagatatta atgagcgatt tatgctggaa tctatatctt tggaattgct
    63361 taaacgacag ttagtttcga ttgataaaat gacttcatta gatggtatca aaattgggtt
    63421 attgaattca agtgaagcaa tcatggttgt tcgagacttc ataaaaacaa aacaaaatat
    63481 cccaatcatt ttagaccctg ttttggcatt caaagaaact gaagctgtga tcaatcaatt
    63541 gtatatttcg cagctcatcg aagaactttt tccacttgtt acgatgatta cgcctaattt
    63601 ggcagaagct gctttacttt ctggtcaagc tcttccctct tcgttaacag aaatgatcca
    63661 tctcgctaaa gaactcactg cttcaggaac aaaaaacatt gtgatcaaag gcggcacaag
    63721 gatggctgga gatgaagctg tggatcttct ctattcaatt gatcattatg aggtattttc
    63781 tcataaaaaa atcgacacta caacagtaaa cggggccggt tgtgcctttt cttctgcaat
    63841 aacagcgaat cttgtgttag gggcagattt taccaaagca gtagcagaaa gtaaaaaatt
    63901 tgttgcccat tgtatttcta atggcgtgct gatgaacgat cataccggaa gtgtctggtc
    63961 tggcggtatt caaaaaggag gaatgagaaa tgaaaactaa gcaattaacg atctatgcta
    64021 tgctgattgc gttgacagtt gccctttcat taacaatttt gatccctgta ccggctacga
    64081 atggatttgt gacattatgc gaggccggga tctataccgc tgcttttctt tatggtccaa
    64141 cgggtggatt agttgttggt gcttccagtg gtctattgat cgatttgatt tcaggatatc
    64201 cgcagtgggc agttttttca ttagtgatcc acgggttaca aggatatgtt gctggttctt
    64261 tcagcaaatc ttctactcgt atgtggggat tagggctttt attaggaagc ttagttatga
    64321 tcatcggcta tggtatcgcc ggttggattt tatatgattg gccggctgga gtggcttcta
    64381 taccaggaaa catcttccaa aatctgattg gaatgggact tgcctttcct ataactgcaa
    64441 gtcttcaacg tattcaagca cggaagcaat attaattgaa aaagaaggtt ttagaaaatg
    64501 aagttgtcag aaaaagaatt aaaagaccaa ttgactgaaa tagttaatga catattggct
    64561 gaggcacact tgaaaaaagg tgatcttttt gttttaggat gcagcacaag cgaagtcgtt
    64621 ggaggacata ttggaaaaaa ttccagtgcg gaagttggtc aatggatcat ccgtacgtta
    64681 aaagagttgt tggatcctaa agaaattgcg ctagctgtcc aaggttgtga acatttgaac
    64741 cgagcattag tagtagaacg aacagtggca gaagcaaaaa atttcgaaat tgtctctgtt
    64801 gttcctgcac tacatgctgg aggtgcttgt tctgttgctg cttttgacca gttcaatgat
    64861 cctgtcgaag tggaacacgt agttggacaa gccggtatcg atatcggtga cacatcaatc
    64921 ggtatgcata tcaagcatgt ccaagtacct gtcagacctc gtttgaaaac ccttggacaa
    64981 gctcacgtga cagcccttcg ctctcgtcct aaatatatcg gtggtcctag agcgaattat
    65041 tgaaatgagt aattgacaga gcaatctgaa aaacttcctc gcaaagtcca actaatgaac
    65101 tttaacgagg aagttttact tgtttgtttt atataaccga gcttcataag gtgctagtat
    65161 gcctttagct tcaaattctc cgctagagga atttgacaag agtaatgtcc aggactgatc
    65221 tgcttctttt ggaagttcat agtcagcaag tgattctgtt aagtttacca taataagata
    65281 ttgttcatct tctaaatatc tttcatagac ataaagttgt ggatgttcat gaagatattc
    65341 tttataagaa ccgtatatca gacctttatt ggtttgtcta atgcggatca tgttcttata
    65401 gaattgcagg accgactctg gttcatattc ttgttgttct acattgatcg ttttggtatt
    65461 tggattagtg atcagccaag gttcatgtgt agaaaaacca gcatattgtt ccgaagtcca
    65521 ttgcatgggt gtcctgctgt tgtctctcgt tgtttcacga atgatatcca atgcttcttc
    65581 tcttgtctta ccttccgcaa gcagcctttt ataaagtctt ttcgaatcta ctgcatcaac
    65641 ttgatcgatc gattcaaagg gcatattcgt cattccgatt tcctgtcctt gataaataaa
    65701 tggcgtacct tgcaaaaaga aatacatcat agcgattgct tttgcagaca tagcccaaaa
    65761 atcgggacga gtatctccaa acactgaaac ggcacgagga acatcatggt tttccatata
    65821 taaagcgttc catccgatac catccaatga agtttgccaa gcgcttaatg catgtttcaa
    65881 tttaaggaca tctaattgcc cttctttttc ttgctgccag atatggatat gatcaaattc
    65941 gaatatcata tcgaaataac cgtctttccc tacccattgt ggtccttctt ctgcagtgac
    66001 accacttgct tctccgacag tcatgatatc gtactcttca aaaacatgtt tcaaatcggt
    66061 caaatgttct tcgattcctg agacattttg aaagggtgag aaagggttct ctgtggcttt
    66121 tactgaatat tcatcttttt ttacatgaga aatcgcatct aaacgaaatc catctatccc
    66181 tttatccaac caccatcgga tcatattgaa tagttcttct ttcaatttct cgctttccca
    66241 atttaagtct ggttgttctt ttgcaaaaac atgcagataa tactgtccag tttcatgaga
    66301 atactcccaa gcagaaccgc cgaaaatcga tgtccaatta ttcggttctt tgccatctac
    66361 cccgtcgacc caaatgtaat aatcgcgata cgggctttct ttagattttt tcgattcttc
    66421 aaaccattga tgctgatccg aactatggtt gatgacaaga tccataatca ctttgatccc
    66481 taattcatgt gcttctttta gcaataaatc aaaatcttcc atcgttccga atctctcttc
    66541 tatatcttga tagtcactta cgtcataacc attatcgaca aatggagatt gatagatcgg
    66601 attgatccag ataaagccga taccaagatc tttcaaataa ttaagttttt cacggatccc
    66661 attgatgtct ccttgaccat cgccattgct atctttgaaa ctagcaggat agatttgata
    66721 gcctactgct tctttccacc atttttgttc ttgtatcata tcgtttcact cctttaacgg
    66781 tccctcttga ttataaccga ttctatttta gaagtatcct tgttaccggt aactagaaac
    66841 gaaaagctca acgaatctgc taagtgcgtt cgttgagctt tattttatca tagatagcgg
    66901 gcaatcagtt gaatcatttg cttgggaaat tcattcaagt cagtaatatc tacaaaacga
    66961 ctttcaccgt agatcgactt gatttcttct ttgtcttggc caatagctgc ggagacaaat
    67021 aggattcctt gacgttcata ttctttcaaa acagactgga tatcttcttt tgcttttttg
    67081 cctgtataat ccggcaatgc ttttggctga ccgtcactaa tattaagcaa cagtttggtt
    67141 gttgcggatt gtctgtttaa tttttctgct gctaaacgaa gtgcagcacc atcacgattg
    67201 ttttgccttg gtttcatagt gactaatttt tcatttaacc attgaaatcc gtcttcaaat
    67261 tctttataag aaaacaaaga agtcttttct cttgacgaac ggtctgctgt atctccgtaa
    67321 atcaacaatg ggatatcgac tttcttagaa aattcggcaa tggcaatcgc tgcttttttt
    67381 gccgcttcga tccgatcatc ccgtatcatt gacccagatt catcgatccg tacagctacc
    67441 gctaaagaag gttgctcatg tggcgggttc tttttgtcaa aatttctcaa atcaccatat
    67501 gctactttgc ttgcattgaa ccgctgacct tcgtattttc ctttttggta cgtctctgtt
    67561 tgttcattat ccagtaactc taaaatttgc cggctaagtg attctactat tggcattact
    67621 tgcatgtgta gctcacgtgc ttccggcgtt tttccttgga aagctggtcg gtgaacgatg
    67681 agaccttctt tttcatgaat cgtttccttc atgatttgtc gcgcttcttt attcagttgc
    67741 ttcttgattt ccttatcatg tgcttccatg tcagaagcag acatttcttg agaatcatct
    67801 cctgtttgtt gttctttttg attttttcct tcgatatcgc tactgcgttc tttttgcatt
    67861 tgaggaggaa tctcgttttt ttctattgtt tcttcttgtt tctcttctcc ctcatttggt
    67921 tcttcagcag acaacgattt cttttcatct aagcgcagaa tttcttcagc attttcagtg
    67981 atgtttcgtt catttgcttc ttcggcgcta atggttcttt cttttgtatg ctcagattga
    68041 ggatgacccc atttttcttt tctcaatatg tcctgaagtt ccagtagcaa tccactgtca
    68101 gacaaagctt gttcaagaat tgccaattca tctgaatcag tggataattt atacaaaact
    68161 tttggataaa tcgactggag taaatcttgt ccttgcttat aggtatttgc ccaataaaaa
    68221 taagaacgca ttcctgctac acctttgatg gcgttagctt ttgcagtttc atccaacaaa
    68281 cggataatct ctgccatctt taagagaagt tcttgttttt ggatcatagt tttcgccatc
    68341 gcacgttctg ccatgacttc taaggcagga agatccattt tttccgcatg ttgcatccga
    68401 tctcttaaag attcatttaa tgggcgattt ccttggtaat ttcgatttgt agtgatcaca
    68461 atgacgcaat ctggatggcg acgaatgatt cctgtcggta aattcaacat cccatttggt
    68521 tcgagcgctg agttaagtgc cacaagaaca gaggcatctc ggatgacagt cggctcttga
    68581 atctctaata aatagccttt ttcaatcgct cgaacgattt cagaagggta atagctgtaa
    68641 tggattgcat ctggataatc agcttcgatc ctttgaacta gctctgctaa tttttcttta
    68701 gcagaaaaag gatcaatgcc gtaatggttc tggatcagtg tcagtacagc ttcaagtgta
    68761 tctgtttggt aaatagcttc tagtaattct tggtcttgtt ccttattaga atctactaca
    68821 ggtaataagg ctccgaaaac atctgatttg tccatatctg caaaacaggt cacttttgta
    68881 tagggaagtt gcagatcagc agatagtgct ttggctaatt gtgtcttccc cgacccggca
    68941 tctccttcca aaagtacgtt actgattttc atttcaggat cttgccaatt ccctttgatt
    69001 tcggaggcga tccgccattc ttctgcactt gtccgatggc ttttcggttt cttccagatc
    69061 atttgctttt ctagccgtga aaaggttcgg ttttctgtta gtgagaaatt ctccatgctt
    69121 ctcttcccct tttgatcatc tctgtctatt aatatcctag ctcatctaaa agacgtttta
    69181 aaatacggaa tcgttcccca atcagttctc cgtctttttg tagtgtatcg taatacaaaa
    69241 tgatgtcttc atcgatcttt ggatttttgg tatctacttc taccgtcatt cggttccctt
    69301 gtaatccaat gatgtctacg actggatttt ctggatcata aaatttttca taacgataag
    69361 catcttggaa gtataaactt ccttgcgcag ctaagatcgc taaatctaaa atacgtgtga
    69421 ttggtaattc ttccgactgt cgtgaccatt tttctccagt atgtcgccat accttgcctg
    69481 agatatcgac ttttccacga tcattccact gagcaaggcc cagtgaaagc ccttttgcat
    69541 ccatatttaa tgcatttctt ccatcaactt tatcatagtc ttctacaacg attactggtt
    69601 tatgttttaa atttgttggt atttccatta ttttaactcc tcatcctttt atttttagta
    69661 aataagtaaa ttagtagttt actcatttac taaactatat gcgacattat agaggattga
    69721 agtgaggatt gcaaagaaaa agagcttcat tgatagaaat aaaaacacca tctcgttctt
    69781 ttacacgctc attagtaaca tgtagaaagt ccaagatgat gttggaat
//
LOCUS       DBNIBO010000002        66496 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.002, whole genome shotgun sequence.
ACCESSION   DBNIBO010000002 DBNIBO010000000
VERSION     DBNIBO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 66496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.002"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            148..297
                     /locus_tag="AC1Y77_000062"
     CDS             148..297
                     /locus_tag="AC1Y77_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HIQ1573675.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            308..1822
                     /gene="dltA"
                     /locus_tag="AC1Y77_000063"
     CDS             308..1822
                     /gene="dltA"
                     /locus_tag="AC1Y77_000063"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HIQ1573676.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1819..3024
                     /gene="dltB"
                     /locus_tag="AC1Y77_000064"
     CDS             1819..3024
                     /gene="dltB"
                     /locus_tag="AC1Y77_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HIQ1573677.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3069..3302
                     /gene="dltC"
                     /locus_tag="AC1Y77_000065"
     CDS             3069..3302
                     /gene="dltC"
                     /locus_tag="AC1Y77_000065"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HIQ1573678.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3305..4573
                     /gene="dltD"
                     /locus_tag="AC1Y77_000066"
     CDS             3305..4573
                     /gene="dltD"
                     /locus_tag="AC1Y77_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HIQ1573679.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4714..5796
                     /locus_tag="AC1Y77_000067"
     CDS             4714..5796
                     /locus_tag="AC1Y77_000067"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIQ1573680.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5796..6599
                     /locus_tag="AC1Y77_000068"
     CDS             5796..6599
                     /locus_tag="AC1Y77_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIQ1573681.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6669..7409
                     /locus_tag="AC1Y77_000069"
     CDS             6669..7409
                     /locus_tag="AC1Y77_000069"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1573682.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7420..8424
                     /locus_tag="AC1Y77_000070"
     CDS             7420..8424
                     /locus_tag="AC1Y77_000070"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HIQ1573683.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8469..10313)
                     /locus_tag="AC1Y77_000071"
     CDS             complement(8469..10313)
                     /locus_tag="AC1Y77_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573684.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10516..11043
                     /locus_tag="AC1Y77_000072"
     CDS             10516..11043
                     /locus_tag="AC1Y77_000072"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIQ1573685.1"
                     /translation="MKTIPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIED
                     KEMSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKA
                     DTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNT
                     FVELKKKRRRTQKFL"
     gene            11171..11854
                     /locus_tag="AC1Y77_000073"
     CDS             11171..11854
                     /locus_tag="AC1Y77_000073"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIQ1573686.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12163..12236
                     /locus_tag="AC1Y77_000074"
     tRNA            12163..12236
                     /locus_tag="AC1Y77_000074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12197..12199,aa:Arg,seq:tct)
     gene            12395..12754
                     /locus_tag="AC1Y77_000075"
     CDS             12395..12754
                     /locus_tag="AC1Y77_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HIQ1573687.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            12877..14508
                     /locus_tag="AC1Y77_000076"
     CDS             12877..14508
                     /locus_tag="AC1Y77_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIQ1573688.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(14570..15211)
                     /locus_tag="AC1Y77_000077"
     CDS             complement(14570..15211)
                     /locus_tag="AC1Y77_000077"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIQ1573689.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    15397..15473
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15493..15801
                     /gene="rplU"
                     /locus_tag="AC1Y77_000078"
     CDS             15493..15801
                     /gene="rplU"
                     /locus_tag="AC1Y77_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIQ1573690.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            15828..16172
                     /locus_tag="AC1Y77_000079"
     CDS             15828..16172
                     /locus_tag="AC1Y77_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIQ1573691.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            16185..16478
                     /gene="rpmA"
                     /locus_tag="AC1Y77_000080"
     CDS             16185..16478
                     /gene="rpmA"
                     /locus_tag="AC1Y77_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIQ1573692.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            16912..19719
                     /locus_tag="AC1Y77_000081"
     CDS             16912..19719
                     /locus_tag="AC1Y77_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1573693.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            19910..20401
                     /locus_tag="AC1Y77_000082"
     CDS             19910..20401
                     /locus_tag="AC1Y77_000082"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HIQ1573694.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            20504..21493
                     /locus_tag="AC1Y77_000083"
     CDS             20504..21493
                     /locus_tag="AC1Y77_000083"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HIQ1573695.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            21526..22329
                     /locus_tag="AC1Y77_000084"
     CDS             21526..22329
                     /locus_tag="AC1Y77_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HIQ1573696.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            22350..23264
                     /locus_tag="AC1Y77_000085"
     CDS             22350..23264
                     /locus_tag="AC1Y77_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1573697.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            23347..23721
                     /locus_tag="AC1Y77_000086"
     CDS             23347..23721
                     /locus_tag="AC1Y77_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HIQ1573698.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            23860..24105
                     /locus_tag="AC1Y77_000087"
     CDS             23860..24105
                     /locus_tag="AC1Y77_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIQ1573699.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            24277..25341
                     /locus_tag="AC1Y77_000088"
     CDS             24277..25341
                     /locus_tag="AC1Y77_000088"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIQ1573700.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            25587..26012
                     /locus_tag="AC1Y77_000089"
     CDS             25587..26012
                     /locus_tag="AC1Y77_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIQ1573701.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            26005..26454
                     /gene="nusB"
                     /locus_tag="AC1Y77_000090"
     CDS             26005..26454
                     /gene="nusB"
                     /locus_tag="AC1Y77_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIQ1573702.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            26551..27405
                     /locus_tag="AC1Y77_000091"
     CDS             26551..27405
                     /locus_tag="AC1Y77_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIQ1573703.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            27406..28743
                     /gene="xseA"
                     /locus_tag="AC1Y77_000092"
     CDS             27406..28743
                     /gene="xseA"
                     /locus_tag="AC1Y77_000092"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIQ1573704.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            28748..28972
                     /locus_tag="AC1Y77_000093"
     CDS             28748..28972
                     /locus_tag="AC1Y77_000093"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIQ1573705.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            28974..29864
                     /locus_tag="AC1Y77_000094"
     CDS             28974..29864
                     /locus_tag="AC1Y77_000094"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIQ1573706.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            29873..30691
                     /locus_tag="AC1Y77_000095"
     CDS             29873..30691
                     /locus_tag="AC1Y77_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIQ1573707.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVASNAHEQFDHK"
     gene            30798..31247
                     /locus_tag="AC1Y77_000096"
     CDS             30798..31247
                     /locus_tag="AC1Y77_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIQ1573708.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            31265..32947
                     /gene="recN"
                     /locus_tag="AC1Y77_000097"
     CDS             31265..32947
                     /gene="recN"
                     /locus_tag="AC1Y77_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIQ1573709.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(33002..33118)
                     /locus_tag="AC1Y77_000098"
     CDS             complement(33002..33118)
                     /locus_tag="AC1Y77_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HIQ1573710.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(33191..34147)
                     /locus_tag="AC1Y77_000099"
     CDS             complement(33191..34147)
                     /locus_tag="AC1Y77_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIQ1573711.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            34318..34686
                     /locus_tag="AC1Y77_000100"
     CDS             34318..34686
                     /locus_tag="AC1Y77_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HIQ1573712.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            34856..35287
                     /gene="mraZ"
                     /locus_tag="AC1Y77_000101"
     CDS             34856..35287
                     /gene="mraZ"
                     /locus_tag="AC1Y77_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIQ1573713.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            35308..36267
                     /gene="rsmH"
                     /locus_tag="AC1Y77_000102"
     CDS             35308..36267
                     /gene="rsmH"
                     /locus_tag="AC1Y77_000102"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIQ1573714.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            36272..36673
                     /gene="ftsL"
                     /locus_tag="AC1Y77_000103"
     CDS             36272..36673
                     /gene="ftsL"
                     /locus_tag="AC1Y77_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIQ1573715.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            36674..38866
                     /locus_tag="AC1Y77_000104"
     CDS             36674..38866
                     /locus_tag="AC1Y77_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIQ1573716.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            38892..39854
                     /gene="mraY"
                     /locus_tag="AC1Y77_000105"
     CDS             38892..39854
                     /gene="mraY"
                     /locus_tag="AC1Y77_000105"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIQ1573717.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            39868..41229
                     /gene="murD"
                     /locus_tag="AC1Y77_000106"
     CDS             39868..41229
                     /gene="murD"
                     /locus_tag="AC1Y77_000106"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIQ1573718.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            41255..42343
                     /gene="murG"
                     /locus_tag="AC1Y77_000107"
     CDS             41255..42343
                     /gene="murG"
                     /locus_tag="AC1Y77_000107"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIQ1573719.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            42358..43578
                     /locus_tag="AC1Y77_000108"
     CDS             42358..43578
                     /locus_tag="AC1Y77_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIQ1573720.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            43734..45059
                     /gene="ftsA"
                     /locus_tag="AC1Y77_000109"
     CDS             43734..45059
                     /gene="ftsA"
                     /locus_tag="AC1Y77_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIQ1573721.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            45082..46323
                     /gene="ftsZ"
                     /locus_tag="AC1Y77_000110"
     CDS             45082..46323
                     /gene="ftsZ"
                     /locus_tag="AC1Y77_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIQ1573722.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            46340..47017
                     /locus_tag="AC1Y77_000111"
     CDS             46340..47017
                     /locus_tag="AC1Y77_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIQ1573723.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            47031..47630
                     /locus_tag="AC1Y77_000112"
     CDS             47031..47630
                     /locus_tag="AC1Y77_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIQ1573724.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            47654..47932
                     /locus_tag="AC1Y77_000113"
     CDS             47654..47932
                     /locus_tag="AC1Y77_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIQ1573725.1"
                     /translation="MALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLAKIC
                     EPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            47981..48763
                     /locus_tag="AC1Y77_000114"
     CDS             47981..48763
                     /locus_tag="AC1Y77_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIQ1573726.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            48784..49491
                     /locus_tag="AC1Y77_000115"
     CDS             48784..49491
                     /locus_tag="AC1Y77_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIQ1573727.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            49753..52551
                     /gene="ileS"
                     /locus_tag="AC1Y77_000116"
     CDS             49753..52551
                     /gene="ileS"
                     /locus_tag="AC1Y77_000116"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIQ1573728.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(52600..54126)
                     /gene="zwf"
                     /locus_tag="AC1Y77_000117"
     CDS             complement(52600..54126)
                     /gene="zwf"
                     /locus_tag="AC1Y77_000117"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIQ1573729.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(54141..54788)
                     /locus_tag="AC1Y77_000118"
     CDS             complement(54141..54788)
                     /locus_tag="AC1Y77_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIQ1573730.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(54972..55313)
                     /locus_tag="AC1Y77_000119"
     CDS             complement(54972..55313)
                     /locus_tag="AC1Y77_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HIQ1573731.1"
                     /translation="MIKKMIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFS
                     DSQQKQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDES
                     ADTSYKLLSEK"
     gene            55478..56206
                     /gene="sfsA"
                     /locus_tag="AC1Y77_000120"
     CDS             55478..56206
                     /gene="sfsA"
                     /locus_tag="AC1Y77_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIQ1573732.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            56222..57235
                     /locus_tag="AC1Y77_000121"
     CDS             56222..57235
                     /locus_tag="AC1Y77_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1573733.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            57235..58512
                     /locus_tag="AC1Y77_000122"
     CDS             57235..58512
                     /locus_tag="AC1Y77_000122"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIQ1573734.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            58575..61277
                     /locus_tag="AC1Y77_000123"
     CDS             58575..61277
                     /locus_tag="AC1Y77_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1573735.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            61429..61746
                     /locus_tag="AC1Y77_000124"
     CDS             61429..61746
                     /locus_tag="AC1Y77_000124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1573736.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            61776..62096
                     /locus_tag="AC1Y77_000125"
     CDS             61776..62096
                     /locus_tag="AC1Y77_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIQ1573737.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            62204..63664
                     /locus_tag="AC1Y77_000126"
     CDS             62204..63664
                     /locus_tag="AC1Y77_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1573738.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            63732..63953
                     /locus_tag="AC1Y77_000127"
     CDS             63732..63953
                     /locus_tag="AC1Y77_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573739.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            63984..64166
                     /locus_tag="AC1Y77_000128"
     CDS             63984..64166
                     /locus_tag="AC1Y77_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HIQ1573740.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            64166..64579
                     /locus_tag="AC1Y77_000129"
     CDS             64166..64579
                     /locus_tag="AC1Y77_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HIQ1573741.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            64702..65883
                     /locus_tag="AC1Y77_000130"
     CDS             64702..65883
                     /locus_tag="AC1Y77_000130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1573742.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(65954..66118)
                     /locus_tag="AC1Y77_000131"
     CDS             complement(65954..66118)
                     /locus_tag="AC1Y77_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573743.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            66272..66345
                     /locus_tag="AC1Y77_000132"
     tRNA            66272..66345
                     /locus_tag="AC1Y77_000132"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66306..66308,aa:Arg,seq:cct)
     gene            complement(66414..>66496)
                     /locus_tag="AC1Y77_000133"
     CDS             complement(66414..>66496)
                     /locus_tag="AC1Y77_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1573744.1"
                     /translation="HIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        21901 a        11183 c        14183 g        19229 t
ORIGIN      
        1 gaaaaaagca gtgatttgat gtgctatttt tataaaagct atgtgacaaa actgttagta
       61 aaaaaacggt ttctgtaagc tagacacatg tttctttttg tcaaaattag ttacactttg
      121 tgtgaggtta tatgaggagg gaatccaatg aacaaatggc gttctaatcc aaaattgaat
      181 tattggggaa aattcatcat tcgaacaatt ttttatagtg cagtactgct gttcctgatt
      241 tatctgtatc actataaaaa tattcaagga ggaactttta tctataacga attttaggag
      301 gaagagtatg gaaatcaaaa cgattattga agcaatcgat gattggggaa taaatgaacc
      361 tgatcgagtt gcttatcagg cggatgagac gcatactttt ggtgaattga aacaagcatc
      421 agacgcactt gcttattatt tagaaaaaaa ggtagaaggc gatggaccaa ttgtcgtttt
      481 tggcaatttg gagtttgaaa tgattgtctc atttctaggg gttgtcaaat ctggccatgc
      541 ctatttgccg attgaagagc atacacccaa ggagcgtatc ctatcgatct ttcgggtggc
      601 taaaccatct atggtcatca gtatcgggga ctggatagaa gatattcctt cggttcctgt
      661 cataacaaaa aaagaatttc aagaaatcaa gaagatacca gtaggatttt atcccagaaa
      721 tagcgtaaaa ggtacagaaa atttttatat tatttttact tctggtacta ctggagaacc
      781 aaaaggcgta caaatcagtc atgataatct agtcagcttt gttcaatgga cgatggaaga
      841 ctttgggatc aagcctggta tgcatttctt agcccaagcg ccattttctt ttgatttatc
      901 ggtattttct atttatcctg cattagtttc tggcggaatg ctgaagccgt taaataaagc
      961 agttgttcaa gactttcgtc aattattcgc gacattgcca gccttgaagt tgaatgtgtg
     1021 ggtttctact ccatcattta tggatatttg cttgatggaa cctacattca atggagaaaa
     1081 tgtgccggaa ttggacatgt ttttattttg cggagaagaa ttgacgaaaa aaactgcaga
     1141 aagtctactt gatcgattcc caaatgcacg gatatataat acttacggtc caacagaagc
     1201 aactgtagct atttcaagta ttcagattga ccagcatgta ctagatagtt atgaccgact
     1261 accgattggc tacgtaaaaa aagacacgaa agtttctatt gtccaagaag gtaaacctgt
     1321 ttcaaaagga gaaacgggtg aaatcattat tgcaggacca agcgtatcaa aaggttacct
     1381 gaacaatccc gaaaagacag ctgctgcgtt cttttgtcaa aatgacttag caagttacca
     1441 tacaggggat gcaggtagac tagatgaata tgggctttta ttttatgaag gacgtatgga
     1501 tcagcaggtc aaattacatg gataccggat tgaactcggg gatatcgaac attatctgct
     1561 ccaagacaac cgaatcaaac aagctgtcgt cgtaccaaaa taccaaggaa caaaagtaca
     1621 gcagctggtt gcttttgtcg tattagaaaa caaaacgaaa cagccggatt ttcagttgac
     1681 taaatctatc aaagaacaat tactagaagt tgtaatggat tatatgattc ctcagaagtt
     1741 ccattatgtc gatctgctgc ctcagacagt taatggaaaa atcgatcgaa aaaaattaac
     1801 agctgaggtg aatcctgaat gatgttacct catatgatcc cttatgcaga gccgttttat
     1861 ttttttcttt tagccattgc gcttgtaccg atcgttctat ctttattgat atgggaaaaa
     1921 agattaccag tttatcaatc agcagtgaca cttttctttt tatttgtcag ctttggaggt
     1981 aattattggc aacagggagc ggcgctgatt gggtatgttt tgtggcagac gatacttgta
     2041 tggggctatt ttgtttatcg gcaaaggaaa aatcagacag gatggttttt tgccgccgtt
     2101 attttagcga taattccact gtttttaacg aaagtgactc ctttttttga acatggaaaa
     2161 gcatcgttac ttggtttttt aggaatctct tatttaacct ttaaatctgt acaaatgatc
     2221 atggaaataa gagatggcat gatcaaaaca tataagccat ttcgttatat ccagtttctg
     2281 ctgttctttc caacaatctc ttctggacca atcgatcgct ataggagatt tgaaaaagat
     2341 cagctccatc cgcctgttcg tgataaatat gttgatcttt tgggaaaagg cgtccaaaat
     2401 atttttgtcg gttttttata taaattcatt atcggttatt attttggtca agtactgctt
     2461 cctgtagtgg gacgcattgc cttacagcat ggtggtattt catgggcatt agtaggttat
     2521 atgtacgtgt acagtatgta tttgtttttt gattttgctg gctatagctt atttgcagtc
     2581 ggtacaagct acatgatggg atatcagaca ccaatcaatt tcaataaacc ttttttatca
     2641 tggaatatca aagaattctg gaatcgttgg catatgacgc tgtccttttg gttccgtgat
     2701 tatgtatata tgcgattgat gttcttcatg atgaagaaaa aattttttaa aagcaagatc
     2761 gtaatgtcaa atatcggtta ttttgctttg tttcttctaa tgggtatatg gcatggattg
     2821 acgtggtact acatcgtata tggactgtat catgcttgtc tgatctgtct gacagatgca
     2881 tggatccgat ttaagaagaa gcataaagag cagttgcctt ctaacaaatt tacgcatggc
     2941 ttagccgtat ttttgacatt tcaagcagta tgttttagct tcctgatttt ttcaggattt
     3001 ttagataaac ttttttttag ctaacgaata gtgacatatt aatttatata acaaaggaga
     3061 aaaggaaaat ggaagaacaa attttatcaa tcttagaaga aataacagga acagatgaag
     3121 taagaaaaaa tcgtgatatt gatctatttg atgaagggtt gcttgattct ttagcatctg
     3181 ttcaattatt agtcgagcta gacggagagc tggatatcca agtccctgta tctgaattcg
     3241 agcgagagga ttggagcaca ccgaataaaa tcattcaaca agcaactgca ttgaagggtt
     3301 gagtatgaaa aagaaacttt ttgctatttt cggcccggtt gttttagctg ttggcttact
     3361 gtttgtgttt tttacctcct cctatcaagt agatatctct aatccagaaa cgattagacg
     3421 tgcctcttct tccatgtcag tgaacgtgtt gaaaggagat gcgataaaaa attatgcatt
     3481 atctgaaaaa caatatattc ctttttttgg ctcaagtgaa ttaagcagaa tcagtccatt
     3541 tcatccgtct gttctggcag aaaaatacca gcgaaactat cgtccatttt tgctgggagc
     3601 acccgggaca caatcgttaa gccaatatat gatgatgcgt tctgcaggag atgctatgaa
     3661 aaacaagaaa gtcgtcttta ttatttctcc tcagtggttc gttaaaaatg gtgtaaaaac
     3721 tgattacttt aatacgtatt attctgaact tcaaacatac gactggttgt tttcaatgaa
     3781 gaaagtaacg cctgctgatc gctatttagc tagaagatta ttaactttct caaaagtaaa
     3841 agagaatgat acgttgacgg cgattctgca aacgatcaaa aaagggaagc tacctttacc
     3901 tgaatctttg aatcagctgc gcagccaatg gaacatgctg aagagagagg atgaagtatt
     3961 cagcaatatt gggttgaaag atcgacagca aaaaatcgat catgagtcaa aacgattacc
     4021 aaaacaatac caagaaacag aattatccat tttagcaaat caaattggag aacgagaaac
     4081 gacgaataat ccattcgggt tgaaaaacga tttttatacg catcgtatcc gagcccacga
     4141 gccagagctc aaacagtcac aaaaaaattg ggattatcgt ttttcgccag aattttctga
     4201 ttttcagctg gtcttggatc aattagcaaa aaaccataac gaagtattgt tcatcatccc
     4261 accggttaac gagaaatgga gcgattatac cggactatct caagaaatgt tacaaggatt
     4321 tgccaaaaag atcaaatttc aattaaatag ccaaggattc aatcgaatag ctgattttgt
     4381 caatcaagcg ggtaccaact attttatgga agacacgatc catctaggct ggaaaggctg
     4441 gttggctgca gatcaacaga tcagaccgtt tttagaagaa aatcatatta ctgctagtaa
     4501 gtatcatctt gacgatgctt tttttagcaa atcttggcaa catcaaatac cagataaact
     4561 acaattaaaa taaagaaaac aggcgagtgt tgctcgcttg ttttctttat ttttgaatat
     4621 tctgaataaa agataaaaat accaagtttg cctgaacaaa aatcataagc aatggtataa
     4681 tcacaatatg aatatagaaa tgggggaaaa catttggata caaaagaaga acagctactt
     4741 attgatttaa cttctgcaaa aggagtacct ggaaatgaag aagaggtaag agaagtattc
     4801 agagaatatg ccaaaccatt cgctgatgat attttttatg acggtttagg cagtgtgatt
     4861 gccaaacatg gtgctggcgg aggccccaag gtctttattt ctggacatat ggacgaagtt
     4921 ggttttatgg taacaaaaat aaccgaaaaa ggattcttgg aattccaaac tcttggaggt
     4981 tggtgggggc aagtcatgct tgctcagcaa gttgaaatca aaacacgtga agggaaaatc
     5041 gtccacggtg tgattggttc caaaccacca catgttttaa cacctcaagt aagaaataaa
     5101 ccttatgaaa taaaggatat gttcattgat attggcgcat caagccaaga agaagccaaa
     5161 gagtggggca tacgtcctgg agatatggtc actccatata ttgaatacaa acgaatgaat
     5221 ggcagtaaat atttactggc aaaagcatgg gataatcgta tcgggacagc tgtttctctg
     5281 cgagtattag agaatctatc taaagaagca cacccaaatg ttttattcgc aggaagcgat
     5341 gtgcaagaag aagtaggctt gcgaggtgct cgaacaagta cacaccttgt aaatcccgat
     5401 attgcttttg ctttagatac tggtacagca ggagacactc caggaatgac accaaaagaa
     5461 gcagattcga ttttaggaaa aggcccacag attttgattt ttgatgcatc tatgatcccg
     5521 cataaaaaat tattgaattt tgttattgat gtagcagagg aaatggatat tccattccaa
     5581 tatacagtga tttctggcgg aggaactgat gcaggacaaa tgcatttgac aagagatggt
     5641 gttccgtctg tagccattac cgttcctgtt cgttatctgc attcccatac atcagtgatt
     5701 catgaagatg attatttgaa tacagtgaaa ctagtcacag aattagtgaa acggcttgat
     5761 agtgaaacag ttgaaaaaat tcggagttat gaataatgaa taatagaata cctatttctg
     5821 aatatcggaa aaaagcacga gtttctttgg aaaatcaatg gggaatcaat acttggcttg
     5881 ttttcttatc tataatattt acgagcgtca tccagaactt ttttgacggt ttcagtcctt
     5941 ttccggaaga aagcatgcag cagcacttat tgagttttct agtcgataat ttacttttat
     6001 tcgcattcaa ctatgcattg ttttacattg cgctagtagt tgtacggggt ggcagaccaa
     6061 aaagtaacct tttattttca gttttccaaa gaaagtattt tggttcgata cttggcatca
     6121 atttgatcaa ttcagtcatc aactggctac tcaacagttt aatctttctg cccatgtttt
     6181 taatcagcgg agtgagtact tatatgcagc tgcttttcag ttcaaatgcg gtacctacgg
     6241 gtgtcatgcc agaagatttt cttgatatca gctttgtttt atcgatgata gggatgattt
     6301 ttgtttcagc actcatcatg agtattgttg gcggtttatt ccaatttgct gcatggacaa
     6361 agttcgacta tcctgatttg tcggttattc aatgtttgaa atatggctgg tttttattga
     6421 aagatagaat tggtacgtat attttgttgc agctgtcttt tatcggctgg tatttgctag
     6481 gagcaatcgc tttgttcatc ggtcttttgt gggtcattgt ttatgtgaat gtgtcagttg
     6541 cagaatttta tgaacaagca cggattgaaa aaggaggtcc agacgcctat tttacttagc
     6601 atgtaagcag tttattcatc aaaaaaataa cttttgtggt aggataaatc tttgaggggg
     6661 aatgtacgat gaaattaact gttttaggat gcttaggagc gtatccttat aaaaatcaag
     6721 ggacaacagg ttatttgctt caatctgacg ggttcaattt gttgatcgat gcaggtagtg
     6781 ctacgctgat caaattacaa gaacaccttg atccacttga tctagatgct gtgatcctta
     6841 gccattatca tcatgatcat attgctgacc ttggtgtatt acagtattac tggcaacttc
     6901 atcctaagag aaatccggag acattgcttt caatttatgg acacacaaaa gattccattc
     6961 actttgaaga attgacgttg gaaaatgtct caaaaggaca tccttatttt gaagctgaag
     7021 aattaaaaat tggtcctttt ctgattactt ttatggaaac aatccatcca gtagtatgtt
     7081 acgccatgcg tatcgtagaa gaaacaacgg ggaaagtatt cgtatttaca ggtgattcag
     7141 gttatttgga aagtttcgct gattttgcga aagatgctga tttgtttcta gcagatacct
     7201 atctttttga aggaaatgaa catcatcatg cccatttcac ttcaaaagaa gctggagaac
     7261 ttgcgaaatc agctaacgta aagaaacttg tacttacaca cctacctcag tttggtgatt
     7321 tagaacaatt gaaaaatgaa gcgcaaaagg cagcaggcaa agggattgat atagaacttg
     7381 ctgcagttga caaagtgttt aacatttagg aggaagaaca tgatttttgt accaaatgaa
     7441 aaccacgacc caagaatcaa tttagcaatt gaacaatatt tgctgcaaga aatgccttta
     7501 gatgaaccta ttttgctctt ttatatcaat gaaccttcaa tcatcatcgg cagaaaccaa
     7561 aatacgcttg aagaaatcaa tcgagattat gtggaagaaa atggcattca tgttgtccgt
     7621 cgcttaagtg gaggcggagc tgtctaccat gattttggaa acctgaactt tagttttatc
     7681 atgcctgatg atggggattc attccgtgac tttgcgaaag taacacagcc aatcatccaa
     7741 gcacttcatg aattgggtgt tgctggggcg gaattgaaag gccgaaatga tctagtcatt
     7801 gatggcatga aattttctgg caacgcgatg tatgctacca acggtcggat gtttgctcat
     7861 ggaacaatta tgttcgactc tgatatcaat gaagtagtca atgcattaaa agtaaaaaaa
     7921 gacaaaatcg aatcaaaagg aatcaaatcg atccgttctc gtgtaacgaa tatcaaacca
     7981 tttcttccag aagaaaagca aaaaatgaca acagaagaat ttcgtcaaga aatcttgttg
     8041 aaaattttcg gcgtagagtc agtagaagaa gtaaaaacgt acgaattgac tgatgaagat
     8101 tggaaaaaaa tcaatcgaat ctctgaggaa tattatcgaa attgggattg gaattacggg
     8161 aagtctccag atttcaattt ctctcgccaa aaacgttttt ctatcggttc gattgaagtc
     8221 catttgaatg tctcagaagg aatgatcaaa gaagcaaaaa tctttggcga tttcttcgga
     8281 ctaggagata tctctgacgt ggaaaaaaaa ttggttggac agaaatatga taaagcatct
     8341 ctatcaaaaa tagtcagcca aattgatgtg aagaaatatt ttggtgcgat agaaccagaa
     8401 gaattgctta ccttacttta ttgaaaataa aaaatagcgg aatccacata tatggattcc
     8461 gctatttttt atacaggatt cgggtaattt cgagagagtt gaacagtgat ccgatcaatg
     8521 aaagtatctt tttcctgcgt tagtgttcct gtgtaacgat ccgctacgtc tttcatcatc
     8581 attaagtcaa aacgttcctg atcatctaat tcttctgtga tgcctcgact aaataggttt
     8641 gtatcgtaat gtgcactatg ttcgcaacaa atgacgaaat gttcctcttg ttcgtgtatc
     8701 gacaaataaa tttttcgata cagtggattt tcgatggcat gagtttctct aaaggaatgc
     8761 tctaaagcgt ggatcaaaag ttgagccaga tcgtcatcgt tgataggaag ttcttcggga
     8821 agatctgctt ggaaggttat ctgaatattc cgtttggcag ctaacttctg gtaacgcgct
     8881 aagataagat ttgtcacctg gtggttgcaa aaagaacaca gcatttcgaa attttttgct
     8941 tcttctaata tcttttccac atagttttta gcttttgttt ggtccgtatg cattaaatct
     9001 tgcagattct ccatctcttg gacaaattcc aggcgaagtg tatttacttc ttggatatgc
     9061 tgtcgaagct gctcataatg ttgctccaac aaatcatttt tgatttttaa gaaatctact
     9121 tgtctccgat ctttgtcggt atttaagatg tgatcgatta gattgtagct gataataacg
     9181 attagaatga taatatataa aatcgtagac caattgatcg ttgtttgata gatacctaat
     9241 gccgtttgga tgtaaatcaa gcaatggata atggctacca agacgatcca aggtgtacaa
     9301 gctacataaa aacggttatt cttatatgct tcgccaatgc atgcaagtga agtgactaaa
     9361 gtgcttataa cactagcaat attagcaatc ggcattaatt caggaagttc tgcaggagta
     9421 aaagttgcat aaagcagaac gccgataaaa atcaaaagtt ggaacgtaca aaaaattccg
     9481 taccattttt tatagtaaaa cattcggctc caatagtagc agatgatgaa agctgacgtt
     9541 aagataataa ataaatgaga taaaaaagaa tgaacgaatg gttcaaaaag aataccactt
     9601 aaaatatctt ctgatacagc ttcaaatcct aatgccaaag caaagcaact taagagaata
     9661 agtgagtatt caagacgttt ttgtttatac atcaagtaga tcgtatagaa aaaggtccct
     9721 atagcgataa aaacagcgat tatcatcgtc aatacttgcg gcactgattg tgaataaata
     9781 aaactaatca atggtccagt tgttcctaga taaactttcg gtggcagtcc agcatagttc
     9841 tgataaggag atgagactgt aagggtcaat ttttttccga tataatttgc tggtaaatct
     9901 atcacagcca atgagagtcc gggattgctt tcttcctgat aatctttccg ttggtataaa
     9961 atcttttcgc ctagatatac agtaatccat tgatggtttc ctttgattag taaatttgct
    10021 tcgtccaacg gtttttcaag tgtcgtttcc ataatgaatg tttcgtcttt atccacggta
    10081 ggaatcgtac gagtgtactt gctgtcaaac ttaaaatcag gatcatcttt tgtatagaaa
    10141 acccacgaat cttttatttc tttcatttga ttgctgtccg caggagaaaa aataaaataa
    10201 aagaaataag caattcctgt taaaaataac aatgataata taatgattat gctccacttt
    10261 atacgtaaat aaaaagattc ttttttatta tatatgttat taaaaaaaga cataaaggta
    10321 ttcctttcta aaacttgtaa tgttagggta aacgtttttt aaactaacgt caattattta
    10381 taataaaaaa tgagataaag atgaggaaat agaattagaa acatataatt attaaaggga
    10441 aacattttta cagctataat ttgaaaaaaa tttatcgtta ttatatgatt aagaaggatt
    10501 gaataaagaa agaagttgaa aacgatacct gattacaaaa aaataacttt tataagttct
    10561 ataacgttga ttatattttt aattcttatt cttctcgtcg tcttggttcc ccgaattttt
    10621 ggtttgactc cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat
    10681 ctgatatatg tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat
    10741 tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac
    10801 gaagattttt atacaaaagc tgatacaaag acttatattg agcctggagt agtaaaaaag
    10861 agaaatatta tcggtagtcc catctttcag attccatata ttggactagt cctttcaaaa
    10921 acggcatttg catggattca ggggttattc tttacgattg ctttttgcct aacaggaatc
    10981 acagcatgga atacctttgt agagttgaaa aagaagcgga gacgaacaca gaaatttttg
    11041 taagaatgtt aaaaacataa atgtattcca gagatccaac gactcttctg aacatgaaac
    11101 acgagaaaac actcatgcag cttgtagatt tagtcagttg ttcgtataat gtgtgaagag
    11161 gaggagtagt atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag aactattgga
    11221 ttttatgcgt acccagcaaa aaaaattacc gggtgagcta ggcaaactgg aagaagaagc
    11281 tcatgtagca gaagtaccag ttattcccca tgaaacagtt gtatttttga aatttttact
    11341 tggacagctt cagcctgaac gaatcttgga gatcggagca gctatcggtt tttcatcaag
    11401 tgtcatggcc acgacaatcg gagaaaacgc tcatgttacg acaattgatc gtttcgatgt
    11461 catgatcgag aaagctaaaa aaaactatga acgtctagga ctgacagata aagtgacttt
    11521 gcttgaaggt caagcagcag atatcttgcc ggagttgtct ggtccgtatg actttatttt
    11581 tatggatagt gcaaaatcaa aatatattga atttttacct gaatgcttac gtttgttaag
    11641 aaaaggtggc gttttgatgg tagatgacat cttccaagga ggaacgatct tgctcccaga
    11701 tgaagagatt ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt
    11761 tgtgatggat catcccgatt tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt
    11821 aatgacaaag gaatcagaaa caatcgagtt gtgatacatg attatggcag cctgtagata
    11881 atcgattgaa tcgagggctc aattcgaact tgatgtaaaa gtttctatat ttgtaagtca
    11941 attaaaggat acaggaaatc taaataaaca ggagtagcat gaagtcaatg tagctcctgt
    12001 tttcaattga taaccttaga acagctgaag gagaatgtct cgttttaaaa gattgattta
    12061 gtagttgggc caaaataatt cattatttgc taattaaata actatttcgt attgacagaa
    12121 gcaatttaat tatctataat tattgatgtt ccgctgattc ttgtcatagt agctcagctg
    12181 gatagagcat ccgccttcta agcggacggt cgggggttcg aatccctcct gtgacgctaa
    12241 aaaaccgtta gaaactgagg tttctaacgg tttttctttg ttttttgtgc caatattgtt
    12301 tgttttcaaa ggcaaataaa ataaaaaaac atccttatcg agtacaatta taaggagcaa
    12361 aaaaataaaa ctcatcagaa aagaggaaga agatatgacg atactatcta gaattttagt
    12421 gactctagtt gcaattgaat ttttttatat catgtatatc gaaacggtga gaactgattc
    12481 tgacacgaca agtagggttt ttaaaatgag taaagaagag ttaagcagaa aatctgttca
    12541 aacattgttt aagaaccaag gagtgtacaa tggactatta ggagtgggac tactttatgg
    12601 agcttatctt tcttccgcat ctaaagaaat cacaagtatg ctattaatat ctattttttt
    12661 tgttgcatta tatggcagtt tgactagcga tcgttggatt ttactcaaac aaggtgggct
    12721 agctctgttg gcattgcttt ctattttctt ataaatatag aaaaaataag aatttatcta
    12781 acaggaaact ttgtgaaaaa tattacttta tctcatgaaa tatgatattc taatgaatgg
    12841 tattttagaa aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca
    12901 ctaaaaaaag aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg
    12961 atcacagtag ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct
    13021 gatgcggccg caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca
    13081 ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt
    13141 gcggcaacaa agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta
    13201 cttgcagcag gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt
    13261 acggggttta cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt
    13321 ggtgttcact cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga
    13381 tttccgataa acacagcctc tttgatcatg ggagggctag tcattttagg gatgtttatc
    13441 tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca
    13501 gcagttatgc tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg
    13561 atcagtagta atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca
    13621 gttccagcta ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg
    13681 gctggccgaa tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt
    13741 ggaaatctgt tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca
    13801 agcgtggcca tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc
    13861 ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac agattccaat gccagcactt
    13921 gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta
    13981 tttactaata aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt
    14041 atctttgatt taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt
    14101 gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg
    14161 ccagaaacgg aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt
    14221 atgtcttcag aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt
    14281 ttctccatgc gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt
    14341 caagaaaaag cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta
    14401 gaaaaacaac tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct
    14461 catgcgttga cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa
    14521 gaacagacaa gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct
    14581 gtaaaagtga agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt
    14641 agaaaatagc tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt
    14701 aggcatcctt ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt
    14761 tggtaaccgt atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa
    14821 ttaattttgg ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt
    14881 cggaacgagt aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga
    14941 tccgatgagt cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc
    15001 gtgttaagcc agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag
    15061 gaacatgcgt atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat
    15121 gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag
    15181 cttcttgtat cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa
    15241 gaataaaaga aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag
    15301 aagtattgct tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc
    15361 tgttctggta agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca
    15421 agtaataagc acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag
    15481 gtgctttata gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg
    15541 ggacaagcaa tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac
    15601 gaagttatct tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca
    15661 actgttgaag gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac
    15721 aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt
    15781 atcgacgcga tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa
    15841 catttaagcg aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg
    15901 ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg
    15961 tcaacgggat tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag
    16021 gcggttatct ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg
    16081 agattctatt agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat
    16141 atatccaaat taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga
    16201 atctacaatt atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag
    16261 aatcaaaacg tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc
    16321 tttaccgtca acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg
    16381 acactttatt tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa
    16441 aacaagtgtc tgtttatcca gtagctcaag aagcttaatt gaatcttatg attaaagagg
    16501 ttgtgacaag acttttgtca cagccttttt tgtttataaa aataaaagtt gtagtggagg
    16561 ctacgaagtt tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat
    16621 gttttgataa ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa
    16681 ttatatggta aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt
    16741 cgttctttta aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt
    16801 aaatagttga cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata
    16861 gtctatagaa aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc
    16921 aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa
    16981 attgctgaag tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg
    17041 cgcgaaggaa aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc
    17101 gttacgcacg taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct
    17161 gaagaaaaaa atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct
    17221 aacggcagca tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga
    17281 ggattaaatt gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg
    17341 atgtatcatt ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc
    17401 aattgtgctg attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca
    17461 aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc
    17521 ggtatgctgt ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt
    17581 tactttatgg atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca
    17641 aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt
    17701 gttcggcgta tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag
    17761 aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt
    17821 tctttgacag aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga
    17881 caattgaagt caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca
    17941 cccgaaatct cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa
    18001 ttagcaagca accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact
    18061 gtagcaccaa atgatacgat catgaaaatc caatcggact cctatgagtt accttataat
    18121 ctatatgata tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa
    18181 gcaatcaatc atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac
    18241 catggttttt cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata
    18301 gaagtgacca atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa
    18361 aactttattt atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga
    18421 gaagaacggc atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat
    18481 gctgttgcga aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa
    18541 agtgaagact attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc
    18601 agaatcggtg tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt
    18661 gtccaacagt tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat
    18721 ccgaaaacag cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg
    18781 gtcatactct tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac
    18841 actggaattc cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta
    18901 cgaaaggtct cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt
    18961 cgaggttacg cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag
    19021 aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa
    19081 ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg
    19141 attgtggaag tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact
    19201 actgggatta ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat
    19261 caaaatatcg aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa
    19321 ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac
    19381 ttcatcttta ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc
    19441 tctcagacga cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac
    19501 acagcaggaa tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa
    19561 ttgacaaata caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg
    19621 gaagatcaga taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc
    19681 cataatcagt tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt
    19741 ttatagtgta tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt
    19801 aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct aggaggtggt tgttcacaca
    19861 gaaatgatag gggtttctgt gaagtagtga ggaagaagga gttttgcaga tggatattcg
    19921 attggcacgt atagatgatc gtttaatcca cgggcaagtc gcaacagttt ggtcgaagca
    19981 aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc gctcaagata aattgagaaa
    20041 gtttctacta aaagaagctg caccgccggg gatcacagca aatgtaataa ccgtgcagaa
    20101 aatgattcaa atctatcata gtggattatt atcacgagca aaagtgatgc ttttattcac
    20161 caatccccaa gatgttgaag cggttgtccg ctgcggtgta cagttgaagt cgttgaatat
    20221 cggcggtatg agttttactg aaggcaaatc aatgattacc aattttgttt cagtaaatca
    20281 acaagatatt gattcctttc gttacttgaa cagtcaagaa gtggagttag agattcgaaa
    20341 agtacctgct gacaggaaaa tccttttgat ggaactacta gcgaaaatca aaaaggtgta
    20401 gcaagaatgg ttgttggaaa aggtcaaata attctgttgt aatggttagc aattattaat
    20461 atttttgaat ttgttacaac aaaagtaaat aggaggtata aatatggtag ggattatcct
    20521 agcaagtcat ggacaatttg ctgaaggaat cttacaatcc ggttcaatga tctttggcga
    20581 acaagaaaac gtcaaagccg tgattttgaa accaagtgaa ggtccagatg acttgagagc
    20641 caaattggaa gaagcggttg cttcttttga taatcaagac gaagtgttat tcttagttga
    20701 tttatggggc ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc acaaagataa
    20761 atgggcaatc gtcagcggtt tgaatttacc aatgttgatt gaagcctacg catcacgctt
    20821 ttcaatggaa tctgctcatg aaattgcagc acacatcatt gaaacagcaa aagacggtgt
    20881 aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg aaagcagcag ttgaagacgc
    20941 acaacctaaa ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa tcaaatatgt
    21001 attagcacgt gttgactcac gtctgttaca tggacaagtt gcgacagctt ggacaaaagc
    21061 agtgcaacca aaccgtatta tcgttgtttc agatgcagta tcaaaggatg atctgcgtaa
    21121 gagactaatc gaacaagctg ctcctccagg tgtaaaagca aatgttattc ctattagcaa
    21181 aatgatcgaa gtagcaaaag accctcgttt tggaaatacc aaagcattgc ttttatttga
    21241 aaatcctgaa gacgtattga cagctgttga aggtggcgta gacatcaaag aattaaatgt
    21301 tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt ctatgggaca
    21361 agaagacgtt gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg atgtacgtaa
    21421 agtacctaat gattcacgcg ataacatgga tgatattttg aaaaaagcaa aagctgaatt
    21481 agcaaaagcg taataaataa tattaaaatt tataggaggc ttatcatgtc tattatttca
    21541 ataattttag tcgtattagt tgcctttcta gctggtatgg aaggtattct tgatgaattt
    21601 caatttcatc aaccactagt ggcatgtaca ttgatcggtt tagttaccgg taacctcgaa
    21661 gctggtatcg tacttggcgg tacgcttcag atgatcgctt taggttgggc aaatatcggt
    21721 gccgctgtag caccagatgc tgcattagca tcagtagcat cagcaattat tttagtttta
    21781 ggtggacaag gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc tttggctgtt
    21841 gcagggttgt tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt tcatttgatg
    21901 gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat cattgctgta
    21961 tgtttacaag gggttcgtat cgcggtccca gcagcagcgt tgctatttat cccagcacaa
    22021 actgtgcaat cattccttga atcaatgcca gcatggttaa cagatggtat ggctatcggt
    22081 gggggaatgg tcgtagctgt aggttacgct ttggtaatca acatgatggc tacaaaagaa
    22141 gtatggccat tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt aacattgatt
    22201 gctctaggtg ctttaggtgt tgcattagct cttatttact tgaacctttc taaaatgggt
    22261 ggttcatcaa ataatggagg cggaagcaac tctggtgacc ctttaggcga cattttgaat
    22321 gactattaat cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa ttatcaaaaa
    22381 aagatcgttt agctgttgcg tggcgctcta ccttcattca aggttcatgg aactacgaac
    22441 gtatgcaaaa cggtggctgg gcattttcta tgattccagc aatcaaaaaa ttatacaaaa
    22501 caaaagaaga acgttctgca gcattaaaac gtcacttgga attctttaat acacacccat
    22561 acattgcttc tccaatttta ggggttacat tggctctgga agaagaacgt gcgaatggcg
    22621 cgcctgttga tgatgtagcg attcaagggg ttaaagtagg tatgatgggt cctttagctg
    22681 gtgttggtga cccagtgttc tggtttacag tacgtccaat gctaggtgca ctaggtgctt
    22741 cattagctat gggaggaaat atccttggac caatcatctt cttcttaggt tggaacatta
    22801 ttcgttgggc attcatgtgg tatacacaag aatttggcta caaagctggt tctaaaatta
    22861 ctgatgacct ttctggcgga ttgttacaag acgtaacaaa aggtgcttca attttaggga
    22921 tgttcgtatt agctgccctt gtacaaagat gggtatcaat caaattctta ccagttgtat
    22981 caactgtaaa attagataaa ggtgcttaca tcgaatggga taaattgcct tcaggtggtg
    23041 aaggaatcaa gagtgcattt gaacaagtta ataatggctt agccctttct cctgaaaagg
    23101 taacaacatt acaagacaac ttagaccaat tgattcctgg tttagctgca ttaggattaa
    23161 cattgttatg tatgtggttg cttaagaaaa aggtttctcc aatcgttatc atccttggtt
    23221 tattcgtagt cggtgttgtt ggacacgtca tcggactttt ataatccata aaaatatagc
    23281 tcgggcgttg tcccgagctt tttttatata gtatactgtt cataaacaaa tatctggagg
    23341 gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg taattgatgc tacttcgttt
    23401 acgggtctga cagattacgg gcaaatcatg ataggtgata atggttttga gttttaccat
    23461 gcgcgagata gacgcaaata tatccaaatt ccatgggaag aagtggatta tgtactagca
    23521 tcagtcatgc taaagggaaa atggattcct cgatatgcca ttcaaacgaa aaaaaatggt
    23581 acatacacat tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag agaatatgta
    23641 gatgaagata gaatggtaca atcattgagt ttctttgatg tagtgaaacg tggtattcgt
    23701 agtatgttta aacgcaagta aaaaataaag aaaacttcta agaggaggtt ttctttattt
    23761 ttttactttg cagaagaaaa tatgaaatag caaaaccaat tatttgtccc ctttaaatcc
    23821 catatgctaa aattgattag caaagaagga ggttatatta tgagttggtt atggacattg
    23881 attgttggag ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg aaattcgatg
    23941 ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa gtattggaca agcgattttc
    24001 ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc catctatttt aggcgcagtg
    24061 attttggtag cagttgtctc atttttcttt ggaaaacgat cataagtttt tacaaaatag
    24121 taaactaaaa ggagttgcag cgagactgtg actccttttt ggctttattt atttaaaata
    24181 aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag tttgctataa
    24241 tggaaaaaag cgtgcaaaga aagagggagt ttcattatga tgctacgtgt agaaaaattg
    24301 cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg ttaccagtcc gtacaatctt
    24361 cgttacttaa cgaactttac aggtacgaca ggattagctg ttattacttt ggaaaaagca
    24421 ttttttatta ctgattttcg ttataccgag caggcggcag cgcaggctca agggtttgaa
    24481 attattaaaa atgtcggacc tatttttgat gaggtagcaa acttagtacg caaagaagaa
    24541 ctgcactcat tagcttttga ggaaacaaca gtctcatttt tagaatattc ggttttagaa
    24601 gaaatcatcg atgcggaatt agtacctgtt tcacagatga tcgaagaact cagagaagtg
    24661 aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc atattgcgga tatggcatat
    24721 gatcacatcc tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc aaatcaacta
    24781 gacttttata tgcgttctct tggcgcttca ggtgtttctt ttgatacgat cgttgctagc
    24841 ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga acaaggtgac
    24901 ttgatcacat tggactttgg ttgctattac gaagggtacg tttccgatat gacacgtaca
    24961 ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt cttagatgct
    25021 caattagcag ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt agatgcagtt
    25081 gctagagatt atatttcaaa acatggttac ggggaagcat ttggacattc tacaggacat
    25141 gggatcggtt tagagatcca cgaaggacca aacgtttctt ctcgtgcaga aaagcaattt
    25201 gtaccaggaa atgtgatcac tgatgaacca gggatctact taccaggaat cggtggagta
    25261 cgtattgagg atgacttgtt gatcacagaa gatggaaatc gtgtgttgac tcgttcacca
    25321 aaagaattga tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa atcagtgttc
    25381 acagactttg ttatagaaaa tcactcgatt taatgataca ctatagaaga acttttaagc
    25441 tgtaagagat aataaagttg ggtgttttct ccaattcata gcagtttgca gctgatcggc
    25501 ggcagttttc ttaccacgtg atcactatgc cgcatcatag agaattcgga tgtatctcgt
    25561 gcttaggcaa agggaggaat aaataaatgg cagatgaaaa aaatttgatt ttaaacgcaa
    25621 accaagattt aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt atcgggattg
    25681 cagcttctaa agtagaaggc gtatacggaa tgcgcggaac ttttgccagc aatgtcactg
    25741 aattattagg acgtgcagct cacggaaaag gtgtttactt agcaaacgag gaagatggct
    25801 tgaaagtaga tctttactgt tatctagaat atggtgtttc agttcctaaa gtagcaatgg
    25861 aaatgcagga acgggtcagc cagcaagtcc ttttcatgac ggatatcgat ttatctgaag
    25921 tcaatatcca tgtagtagct gttgttcctg aaaaattaga acaaccaaca atcgaagaac
    25981 tttttgaaga cgaagaggaa gagaatgaat aaagaattat caagacacga gattcgggaa
    26041 atggcattgc aggctttatt tccattagac ttcaatgcag acctaacaaa agaagatgcg
    26101 attttcaacg caatcgaatt agaccaccgc gacatgatca acgaagatga atcagagttt
    26161 gttcctgttt atcttgatac gctagttggc ggtgtctgtg caaaaaaaga tgaattggat
    26221 aaagtgattg aaaaacattt aaaaaacaat tggcacatca atcggatctc aaaaatggat
    26281 ttggtgatct tgcgtatcgc gatttttgaa atgacttatg tgacagatgt accagcggct
    26341 gttgctttaa atgaagcaat cgagttggca aaaacattta gtgatgaccg ttcacgtaaa
    26401 ttcgtcaacg gtgttttatc caatgtattg aaagaattag aagctggggc gtaagtccta
    26461 gcttttttct atgaatcatc ataaacctgt tataaatcgg gaaaattcta tggtagaatg
    26521 aaaacacaaa aaatatctgg gagtggttag atggcagaat tgattaacgg aaaagaatta
    26581 gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa aattgaaaga taatggtatc
    26641 catcccggat tagtcgtttt attggtaggg gaaaatccag ccagccaaat ctatgtaaga
    26701 aataaagaac gtgtagcaaa agaaatcgga atccattcat tggtagagcg ttatcctgag
    26761 acgatttctg aagacgaact acttgcagaa attgaaaaat acaatcaaga tcctagtttt
    26821 catggtatat tggtccaatt accattgcca aagcatatag atgaagaaaa gatcctgtta
    26881 gctatccgcc ctgaaaaaga cgtagacggt tttcatccga tgaatttagg cagactcctt
    26941 gcgggtaaac cggacaagat tccttgtact ccatacggca tcatgaaaat gtttgaagct
    27001 tattctatcg atccggcggg taaacgagca gtagttatcg gacgaagcaa tatcgtcggt
    27061 aaaccgatgg ctcagctact cttgatggca gatgcaactg ttactgttgc tcattctaaa
    27121 accgaaaatc tggctgaact ggcacaagaa gcagatattt tagtcgtagc aataggacgt
    27181 ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg tagtgataga tgtagggatg
    27241 aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat ttgatgaagt ggagcctatt
    27301 gcaggctgga tcactcctgt gccaaagggt gtagggccaa tgactatcac gatgctgatg
    27361 tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg aatagatggc acaagaatat
    27421 ttaactgtca ctacattaac gaagtatttg aaaagaaaat ttgatgccga tccttattta
    27481 gaacgcgtat atttaacagg cgaaatatcg aatttccgtt tacgtgccaa tgcccatcaa
    27541 tatttcagct taaaagatga ccatgcaaaa atatctgcta ttatgttcaa aggagcattc
    27601 caaaaattac gattccagcc aaaagaagga atgaaagtcc ttgtcgttgg gcgtatttca
    27661 ttatatgaag cgggaggttc ttatcaaatc tatgtagagc acatggagcc tgatggaata
    27721 ggtgctttat atcaagaatt ggaagaacga aaagaaaagc tgatgaagga aggccttttt
    27781 caagggccta aaaaaatgct gccacgtttc ccaaagcgaa tcgcggtcct taccagtcca
    27841 agcggagcgg ttatccgcga tatcatcacg acggtgaaaa gaagatatcc aattgtccaa
    27901 ttggttttgt tcccaacagt tgtacaagga aaccaagcag cggacaatgt tgtcaggaat
    27961 atccagagag tagaagagat gggcaatttc gatacaatga tcatcggtcg tgggggtggt
    28021 tcgattgagg atttatggcc gtttaatgaa gagcgggttg ctcgggcgat ttatcaagct
    28081 aatacgccgg tgatttcatc tgtaggacat gaaacagata ccacgatcgc tgatttagta
    28141 gcagatgttc gggctgccac tccaacagca gctgcagaat tagctgttcc tgttttgtca
    28201 gaagagttga tgcgcattca agaacggcaa gctagattgc agcaggcgtt tgtacgccag
    28261 atccagcgca aacaagaacg gttcgagcgt gctcgacgtt cctatgtatt tcggcagcct
    28321 gaaagattgt atgaaggtca gaccatcaaa ttagaccagt tgaaacagcg attatatcaa
    28381 gctacacagc aaatttatca tgcgaaagaa aaacaaacga tcgcattaag ccaccaattg
    28441 gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc aagaaactgc ttatctagaa
    28501 aaacgcttag aagagaaaat ccaacaatat atgaaacaac agcagcagcg ttttgctcaa
    28561 gctgttcaat ctcttgattt gttgagtcca ttgaaaatca tgggaagagg ctacagctat
    28621 acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga ttcatcaagg ggatttgctg
    28681 acgatccatt atcaagatgg aatcgttaaa ggacaagtac aagagataga caaaggagaa
    28741 taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa gaattagaaa aaattgtcat
    28801 gcagttagaa caaggagacg ttccattaga atcagctcta gattcgttta aacgaggaat
    28861 ggaattaagc aaacattgcc aagatacatt agctaaagca gaagagacct tgacgaaaat
    28921 gatgacagaa tcaaatgaag aagttgattt tgacggaaac gaggaaagct gagatgagat
    28981 taactgactt ttcagctatt catctacctg ctgtagaaaa agaaatcttg tcttttttag
    29041 atgagcatac aacggataga ggactctatg atgcgatgag ttattcagtg aaagcaggcg
    29101 ggaaaagaat acgtccctta ttacttttaa cggccgttgc ttcctttgat gagcctattg
    29161 atgttcccgt ctatcaagta gcagctgctt tagaaatggt gcatacgtat tccttgatcc
    29221 acgatgattt gcctgcgatg gacaatgatg atcttcgtag aggaaaacct accaatcata
    29281 aagtattcgg cgaagcatta gctatattgg ccggagatgg tttgcttaca ggagcattcc
    29341 agctaatcag tatggcacac ttaggtaatt ctcctaagct tctgctgctt cagcaattag
    29401 ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc tgcagatatc gagggcgaat
    29461 cgaaaaagct ttctttagaa gaattagcat ttattcatga aagaaagaca gggcatttga
    29521 ttcgttatgc cttgctggct ggtggcatat tagcgaagca acctgaagaa atcttattgc
    29581 tacttcaacg attagccgaa catttaggtt tagcttttca gataagagat gatctactcg
    29641 atgtgattgg tacaacgaaa acattaggga aaacagctgg taaagatgaa cgtatggaaa
    29701 aaaatactta cccgcgttta ttaggcctag aaaagacacg tgaggcgttg gagattgagc
    29761 tgttatccgc caacaagatc atagacaaat tagaagagga cgttttttct tttgatggcg
    29821 ggctgatgcg ccagatgatc aagcaatttg atgtggagaa atgagggaaa gaatggaaaa
    29881 agaacgcgta gatgtattag cggtcaaaca aggattattt gaaacaagag aacaagccaa
    29941 acgttcagtg atggctggtt tgatttacaa cgaaaaaaac gagcggtttg ataaaccggg
    30001 agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt aaaaaacttc cttatgtttc
    30061 tcgaggggga ttaaaattag aaaaagcact caaagcattt gatctatctg ttgaaggaaa
    30121 aacattgtta gatattggtg catccactgg cggatttact gatgcagctt tgcaaaatgg
    30181 cgcaaaaatg agttatgctt tagacgttgg ctacaatcag ctagcttgga aaataagaca
    30241 agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt tatgcaaaac ccgaagactt
    30301 taccttagga attccagaag ttgctgttat cgatgtttct tttatttcac taaaactgat
    30361 gctgcctcca ttacatgcta ttttaaaaga agagggagaa gtgattgctt taatcaaacc
    30421 ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga attgttcgtg accctcagac
    30481 ccatcaaaaa gttttagaag acattttgtc atttgcggca caaggtggat atgatgtgct
    30541 ggaattaagt tattctccga tcacgggggg agaaggaaat atcgagttct tggctcactt
    30601 gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt aatatggcag aagtggcttc
    30661 aaatgcacac gaacagtttg accataaata aattgtaact gtgaaaatca agtgagaagt
    30721 atgcggattc ttctttatta taccaaggaa ataagctatc attgattaag aagaatatgc
    30781 taaggagcga agacgtaatg agaaaaaaag atcggcatcg tttgattact cgattgttga
    30841 gtgagcaaga tatccgaaag caggaagaat ttgttgaaat cctgaaaagc aaaggaatat
    30901 ctgttactca agctacgatt tccagagata taaaggaact gaaactgatc aaggtaccgg
    30961 cttcagatgg cggctaccgt tatagcatac cagctgaaac aaacgaagat gtaaatacta
    31021 aattggaaaa actattaaaa gacgctttcg tggcagttga ccaaatggaa aaatttgtta
    31081 ttttgaaaac attgccggga aatgcttcgg cagcagcaaa tttgattgat aaacgttaca
    31141 agaaagaatt attttcgatt atcaatgatg acgataatgt tttgatgatt acacgaactg
    31201 aagaccaggc agttttattg aagaaagact tcctgcatta cctttaagtt gaggagcgaa
    31261 tgtgatgcta caagaaatca gtattactaa ctttgcgatt attccggaat tgcggctttc
    31321 ttttcacgaa ggaatgaccg cgctgaccgg agagactggt gcgggtaaat ccattattat
    31381 cgatgcttta ggtctgttag caggaggaag aggttcaagc gattatatac gtcaaggagc
    31441 agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa caagaaggat tctctgaact
    31501 gatggttgaa ttagggatcg aaacagatga ggacaatctg attgttcgac gggatatgtc
    31561 cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt atcacattag ctaatctgag
    31621 aagaatcggc agttatttag tagatatcca aggacaaaac gaacatcagg aactgctcca
    31681 gcctgaatca catcttgctt tattggatcg ttttggagat gtagctttcc aacagaagaa
    31741 aaagagttat cagcaagaat atatctctta tcgtgagctg gaaaaaaggg tacgaaaagt
    31801 gcagcaaaat gaaaaatcct atgttcagag aatagatatg ctccatttcc agcaagaaga
    31861 aatagctgct gctgatttgc aagtcggaga agaagagaaa ttaaaagaag aaagagaaaa
    31921 acttagcaat tatcaaaaaa ttgctgatgg attagctgca ggttacggag cactgacgaa
    31981 taatgaacaa aacagtgtag atggtgttgg cttggccgtt tcagaactgc aagggatcgc
    32041 ccatctagat gtggagtatg aagcaattta tgaaaatatc caaagtgctt actatttgct
    32101 gcaagatgca atcggcgata tgagtcgtca gattgattta ttggagttag atgagagtcg
    32161 tttagaagaa gttacgcaac gtttagaatt aatccgacag ttgaaacgta aatacggcga
    32221 atccattgag tcaatattgg cttactatga cgaaatcacc gaagagctag cttcttctga
    32281 tttttcagaa ggtcaattgg acaaaatgaa agaggaacta gagcaaaaag agcttctact
    32341 tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa atcgcaaaag aattggaaaa
    32401 atccattctg catgaattga aaagtctgta tatggaaaac actgagtttg aggtacgttt
    32461 cttaaaagaa gaaaatagac aattgaatca tgatggattt gaccaaatcg aattttacat
    32521 tacgaccaat ccaggagagc cactgaagcc tttggtgaaa gtcgcttctg gtggcgaact
    32581 gtctcggatg ttgcttgctt tgaaaactat tttttcttct gagcaaggtg tgacaagtat
    32641 tatttttgat gaagtggata caggtgtgag cggaagagtt gcacaggcca tcgcagataa
    32701 aatttctcaa atatcgaaat actctcaagt tttgtgcatc actcatctgc ctcaagttgc
    32761 tgcagtcgcc gactatcaat attatattgt gaaagcagtg attgatggac gtacgcaaac
    32821 ttctgtttca gagttgaaaa caaaagaaag agaagaagaa attgcacgta tgctggctgg
    32881 aagtgagatt acaaaactca cactcgaaca tgcaaaagaa ctattgaaac tcgcaaaaag
    32941 gacctgagcc acctcgcagt ggttcaggtc cttttcagca gctgatagct atattttatt
    33001 tttacatgac actcattaca gcagtcaata caacagatcc aatcgttaaa atcagcattg
    33061 tccaaatgac aaattttgtt actttagcaa aagtacttgt ttgttttttt tcacgcattg
    33121 ttttcaacct cgtcattaat tagttgattc aagtgtacaa tcttttttcc atttccacaa
    33181 gagggctttt ttataaaaac ttcccttttt ttaaataata ggtagttacg atacaaataa
    33241 ataatgttaa tagaactagc catataaagg catcatccct ttcagcaata ggtaacttaa
    33301 tgttcattcc atagatactg ccgacgattg tcggtatagt taaaataatc gttaaagaag
    33361 ttaaaatttt cataatatta ttcagattat tcgatacgat tgctgaaaaa gtctgattca
    33421 tttgttctac tagttttaat tgaatgcgag aagtagtcat tgcctgctct gtttccacca
    33481 atacatcacg taaatgattt ttcaaagcat gattttcttg gaaaagttta ttttgcgcta
    33541 gttctttcaa tgcatctaaa ttagcagaag tcgcttctct gaaataaacg aggctttttt
    33601 ggatatccat gatttgataa agttgtttgt tttcagtgga tacttgaagt ttggcttcca
    33661 aagtatcgca ttgagattga agcgtagcta aataactgtt gtaagagagt gtgacctgcc
    33721 aaatgaattg aaagaagata ttgagattag gcgaaatatc actttttgga aaggtctggc
    33781 taaagacttt ctttaaaaac agtggttcat gattcgtaat tgtgataagc ttctttttgg
    33841 gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa ataaccgcta ggacttgttt
    33901 tagcaaaagg atattgtaac aataataacg ctggctttgt caactttgat tgattgaatt
    33961 cttctgtacg ggaattttct gcatcgtcca atatggaagt gatgtaatct gacggaattt
    34021 caaattgatg tgtcagctga tgaatctctt cttgattagg acgttctaca caataccaga
    34081 tcgtttggtc agtttccttt ccggtattac gcagtttctc attatctaat gtaaaatatt
    34141 tgatcatgat tttcctcctg ttgcctgttt cttttattat aacggatagg gaattggaat
    34201 acatggaatg tgtttgtaag atatagaata taactgcata aatttaaata aaatgaaaca
    34261 gttgtgtaaa gtatatacag tatacttgaa gcaacgataa aaaaggagtt tacgttcatg
    34321 ggcacattca caccacttat cttgttttgg tatatttacc cggtaatcgt tttgtttggt
    34381 tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa agcccctgat
    34441 ttggcgattc cttttttgtg gggcggtatg catgcattgt ctagaaatac agggacaatt
    34501 tctatcttac cgtttttctt tatttctgtt ttgctaatgg gaatttgtat tgctgttttc
    34561 caagcatatt attatgaaga aattatttat ccgcgttatt tcaagatgac ttggcgtttg
    34621 gtttttctgt tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat actttcttac
    34681 ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc agcttgattt
    34741 ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa aggtggtaga
    34801 aagtggggga ttgtggtaga ctaattttag ttagtggagg aatggggggc tttcaatgtt
    34861 gatgggtgaa ttccaacata atattgatgc aaaaggacgt ttaatcgtgc cctcaaaact
    34921 tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga ttggatggct gtcttttcgg
    34981 ctatccgatg agcgaatggg aaaatctgga agctaaactg aacgaaatgc cgttggctaa
    35041 aaaagacgca cgtacttttg tgcgattctt ctattcggct gccactgaat gcgaacttga
    35101 taaacaagga cgaatcaata tacccagcac gttaagaaat tatgcagctt tgacaaaagg
    35161 ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg gatgagacac gatggcaaga
    35221 tttttctgca gcagcagaag agaactttga cgaaattgct gaaagtatga ttgatttcgg
    35281 actataagaa taggaagagg ctgactaatg actgaaacat ttcaacacta tacagtcatg
    35341 ttgaaagaaa cggtagatgg attgaacatc aaaccggatg gcgtttatgt agactgtaca
    35401 ctcggcggtg ccggacatag tgaatattta ttaagccaac taagttctaa agggcatctg
    35461 tatgcgtttg accaagatca aaaagcaatc gatcacgcca agctgcgttt agcttcttat
    35521 gtcgaacaag gtatggtcac atttatcaaa gcgaacttca gagaattaga agaacgcttg
    35581 agtgagcatg tagataaggt agacggcatt ttgtatgatc taggggtttc ctctccccag
    35641 ctagatgaag cagaacgtgg atttagttat catcaagatg caccgttgga tatgcgcatg
    35701 gatcagtcag ctcctttatc cgcttatcac gtaattaatg aatattccta ccatgaatta
    35761 gtaaagattt tcttccgcta tggcgaggag aaattttcca aacaaattgc tcgtgaaatc
    35821 gaacgagtac gaaaaacaaa accaatccaa acaactggtg agcttgtcga aatcattaaa
    35881 caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag aatttttcaa
    35941 gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt agaacaggca
    36001 atccggttat tgaaagtcaa tggaagaatc agcgtaatca cattccattc attggaagac
    36061 agaattgtca aaagtatgtt taaagaatat agtacggtac aagatctacc accgggactt
    36121 cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa accaatcctt
    36181 ccttcagagg aagagctaac agaaaacaat cgatcacgca gtgcaaagtt acgtattgca
    36241 gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa tgcaagatta
    36301 cgaatatgat ttacctgaat tggtggatca gccggaacaa caaatgcctg attcttcttc
    36361 gaatcaagaa atcattcttc cacaatcacc ggcaaaacga ttaaagcaga tttctgcttt
    36421 ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg ttttaacagt
    36481 tatgctgcgc acaaatatca gcggagctga aaaagaaatc acgtcaatac aaacagaaat
    36541 caatgataaa aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac tttctcgtac
    36601 tgaacggatt aaacaaatcg cagaagaaaa aggattgtct attaatgacg acaatttaag
    36661 gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg tttatggaaa agaaaaatct
    36721 aaatccgatg aacaatcgca aaaaagtagg tatcatttta tttgctacga gtattggatt
    36781 gttcttttta tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg ttgctggtac
    36841 ctcattagct gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa aagcaaaaag
    36901 aggaacgatt tatgatcgta acggtgtggc gctcgcagaa gatgcctcct cttattcaat
    36961 taaagcgata ctctcaaaaa catatacctc tggggataaa aaactgtatg ttgaagaaaa
    37021 gaattttgat aaaatagcag aaatcctgca taagaatctt tctatagaca aaaaagatgc
    37081 actgaatatt ttagaggacg gcgcaaaaaa agagctgtat caagttgaat ttggtagcta
    37141 tgggaaaaat atctcccaag aaaccaagca aaacatcgaa gcggacatga aaaaagaagg
    37201 tgtcgctggt ctttattttg tcgaccatca ggctcggatg tatccaaatg gcgtcttttc
    37261 ttctcatttt attggatacg cagttccaga caaagatgag aatggcttag ttggtaaact
    37321 aggtttggag tctgcttaca atgatatctt gagcggtaaa gatggtaaga ttatttatca
    37381 aaaagataat tttcaaaatc ctctgccagg tacagtggca gaagaagaaa aagctgtcga
    37441 cggacaagat atctacacga ctttagacag cagactacaa agttatttag aaacactgat
    37501 ggatcaagta aatgaagaat atcagccaga agaattgaca gcagtcctga tgaaagcaaa
    37561 aaccggagaa atcttggcaa tgggacaacg acctactttc aatcctgaaa caatggaagg
    37621 attgactgga aaagatgcta tctggcgtaa tttcttggta caagatagtt atgaaccagg
    37681 atcaaccatg aaagttttca ctactgcagc tgctatcgaa gaaggagaat tcaacgagaa
    37741 tgaaaccttc caatctggaa aaatccaagt agaagatgct acgatcaatg accatgactt
    37801 tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca tggtcaagta acgtcgggat
    37861 ggtcatactg gaacagcgat taggcggacg atggtataac tatttacaaa aactgggatt
    37921 tggacaaagt actcattctg gattagatga tgaagtaaac ggtgctttac ctacattaaa
    37981 tattgtcgac cgagcaatga gtgcttatgg acaagcggta ggtgtgacga atttccaaat
    38041 gatgaaagct ttcacttcta ttgcgaataa cggtacaatg attcaacctc gatatatcag
    38101 caaagtggtg gatccccaaa ctggagaaga gcgaacaact caaacagaag ttctagggca
    38161 acctttttca aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg ttgtcgaaag
    38221 cgaaaactac ggtattgctt atggtgtata tagtgtacct ggatacaatg tatcggcaaa
    38281 aaccggaaca gcccaaatcg cttctgatac agggggatat caaacaggag atacagccta
    38341 cctgtattcg attgtggaga tggttccttc tgaagatcca gactatgtct tgtatctaac
    38401 aatgaaacac cctaagacat atgatcgaat ggcattggct aaaatcgcaa atccattgat
    38461 gaaacgggca atggatttta aagaaactga agaagattca gatacagaaa caaaaacaga
    38521 aaaagtatcc gtagccgatt atcgtaatct tgaagcggac gttgcagcag ctgatgctca
    38581 aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa aacaatctac
    38641 tgccaacggt gatcagttga tctctggaga aaaactgatc ctatatactg gtggtgataa
    38701 gctgatgcct gacgtaacag gatggtcaaa agcagatatc atgaagctgg ggaaaatcct
    38761 cggtattgaa gttagttttg atggagatgg ttattgtgtc aaacaagaac ttgctccata
    38821 tgaaaaaatc actgaagaca aacttagttt tactttagaa gaataagaag agaaatagga
    38881 gagaaaagag catggaatgg acacaagcat tgattcccat tgtcagcagt tgtgcaatga
    38941 caattgccgc aatgccgcta tttattggtt attttcaaat gaaaaagcaa ggtcaggcga
    39001 ttcgtgagga gggaccaaag tggcacaata gcaaagccgg aactcctaca atgggcggct
    39061 tagtgtttct gatcggctct attttgacag gaatttgggt tggagcttgg caaaaacagc
    39121 taactcctac tcttttcatc ttgctttttg tattagcgtt atacggagta atcggctttc
    39181 ttgatgattt tataaaaata ttcaagaaaa gaaacatggg actgaactcg aagcagaaat
    39241 tgattggaca gattctaggg ggaatcatct tttatcttgt ttaccgttct gaaggttatc
    39301 caggtgtatt gaatttcttc ggactagatc tacctttagg aatcatttat ggtattttcg
    39361 ctctcttctg gcttgttgga ttttcaaatg cagtaaatct gacagatggc atcgatggat
    39421 tagttgctgg cttaggaagt atctcttttg cagcttacgg tctaattgct tggcatcagc
    39481 atcagtatga tgtattaatc atttgcttaa gtgttttagg aggtttactg ggtttcttcg
    39541 tctacaacag aaaacctgcc aagattttta tgggagatgt cggttcatta gcgttgggcg
    39601 gacttttagc ggcaatttct ataatgctaa accaagaatg gacattgctt ttgattggtt
    39661 tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt tacttccttt aagcttacag
    39721 gaaaacgaat ttttaaaatg tctccaattc atcatcactt tgaaatgtgc ggatggtcgg
    39781 agtggaagat tgatacagtt ttttggttga tcagcattgt gacttcgttg atcacattat
    39841 ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat caacatatga aaataagaaa
    39901 gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt acatgaattg
    39961 ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga agcacaagag
    40021 ctgctttctt tagggataaa agtaataaca ggtagtcatc caatcgaatt gttggatgaa
    40081 gaattttcat tgatggtcaa aaatccaggg attccctaca gtcatccttt agtagcaaaa
    40141 gcccaagaaa tgggtattcc ggtaattacg gaagttgagc tagcttacga agtagcggaa
    40201 tgcccaatta ttgggattac aggaacgaac ggaaaaacaa cgacaacaac gatgacagga
    40261 ctactactga atgcaggagc agatcaagga attgcacgtt tagcgggtaa tattgggtat
    40321 ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata aaattgtgat ggaattgtct
    40381 agcttccaac tcatgggaat cacagatttc cgccctcata tagcggtgat taccaatatc
    40441 tatgaagcac atattgatta tcatggtaca agaaaagaat atgtcaaagc taaatggaac
    40501 cttcaaaaaa acatgacaga aaaagattat ctgatcctta actggaatca gtcagaacta
    40561 caagaattag cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa agaagtgttg
    40621 gaagacggtg tgtatgctga cgattactca atatactata aaaaagaaaa aataatggaa
    40681 atatctgaac ttggcgtacc aggtaagcat aatgtagaaa atgcactagc tgctatttct
    40741 gtagcaaaat tgtatggtat atcaaatgaa gctatacgag aaacgttgca ttttttccat
    40801 ggcgtaccac atcgtacgca atatgtagga gaaatccaag gcagaaaatt ttataatgat
    40861 tcaaaagcaa ccaatatttt agcgactaaa atggctctaa gtggatttga aacgtctaag
    40921 gtcgtgttat tggctggcgg attagacaga ggaaatacat ttgatgaatt aattccttca
    40981 ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt gatggatgct
    41041 ggaaaaaaag caggcatcga gacaatagta acagccgatt ctgtagaaca agcagttcct
    41101 ttagcattag aaaacagtac agatggtgat gtagttttat tatcaccagc gaatgccagc
    41161 tgggatcaat acccaaattt tgaaactaga ggaaacagat tcatggaagc tgtgaaccga
    41221 ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa attttggtta caggcggagg
    41281 aacaggggga catatttatc cagctcttgc ctttgtcaat tatgtaaaga ctcaagaacc
    41341 gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa tcgtccctga
    41401 tacaggaatg cctttccata ccttggagat tcaaggattt aaacgaaaaa tttccatgca
    41461 taacatcaag acggtccgat tgtttcttaa gagtatccgt caagcaaaaa aaatcctcag
    41521 tgattttcaa ccagatatcg ttatcggtac tggaggatat gtttccggag cggtagtgta
    41581 cgctgcagcc aaaatgggta ttccaacagt tatccatgaa caaaacagtg taccaggtgt
    41641 taccaataaa tttttaagcc gatatgtgga tcggatcgct ctttcattta aagatgcggc
    41701 ttcctttttt cctgaaaaca aagcagttct tgtaggtaat cctcgagcac aagaagtagc
    41761 agatactaaa aaatcagata ttttgaaaac atttgatttg gatccagaaa agaaaacagt
    41821 cttgattttt ggcggtagtc aaggagcact taaaatcaat caagcagtat cagttttttt
    41881 aagtgaatat attccttctt cttaccaagt tttgtatgct tcaggagata gatattatga
    41941 agagatccta aataaaataa atcctgaaat acaaaatatt agtgtccagc cttatatcaa
    42001 aaatatggca gaggtcatgg cttccagtga tctgctggtg gggcgtgctg gtgcaacttc
    42061 gatcgcagaa ttcacagctc tggggttacc ggcagtgttg gttcccagcc catatgttac
    42121 gaatgatcat cagactaaaa atgcaatgag tcttgttcac gcaggagcag caaaaatgat
    42181 tgcagataat gaactaacag gtgaatcact aagtcagaca gtcaacgaaa tcatgggtga
    42241 tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc ctgatgcttc
    42301 aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa taaaggcggt ggtcttagtg
    42361 agtaagaaaa aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa caatttgact
    42421 ccttggcaaa aggctaaccg aaaatatctg gcagaacaag agatgaaaaa ccaagagcca
    42481 aaaaaagaag acataacatt agatacaccc ctttcagatt ctgaagaaga aaaaacggtt
    42541 caagaggaac agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt tgaagagatc
    42601 aagcaaaaga aaaacggtgg accatataac ggttcttttt tgaaccggct gcccaattta
    42661 aaaagtcaac ggcacaaagt actatttcgt cggttagctt tgatcatcac tgtattgggt
    42721 ataccgctga tttttttgat ctattatgtt tctccttata gcaaattaca agcagttgca
    42781 gtatcaggca ataagacagt caacagccaa gaaatcatct ctgatacaaa attgtcatta
    42841 ggagaaaatg tttggggaca gtattttcat cgatctactt atatcgatcg ccttaaaaaa
    42901 gcacagccaa gaatcgaaac agcaaatata catttcaaag ggatgaatga attcgatttg
    42961 gatgtaacag aatataaaga gattgctttg atcgcaaaaa acaatcaata ctacccagtg
    43021 attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa tctccctatc
    43081 ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa caaagcaata caatcagtta
    43141 tcatcagaat tacaaaaagc aatttctgaa atcaagtaca cgccaagagc aagcaataaa
    43201 aatctgatcc aattgaatat gaatgacggc aatcaagtta ttgtaaatat caataatctt
    43261 gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata tggatgaaaa aggtgtgatc
    43321 gacatggaag ttggtatctt ctcgcatcct tacggtagca ctcaagaaac ggaagaatcg
    43381 ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa cgaatcaagt
    43441 gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa cggatcatct
    43501 gattcaaatg attcaagtga aacaagtcaa atagaagata atccaagtac aactccgtca
    43561 agtttaaatg aaaattgata aaacaacttt aaaaagtaag aatgtacttt ctaaaaacca
    43621 gtaatttatg gtagaattgg aagcagtgaa tatatctatt aataagtata ttttaaaacg
    43681 gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa
    43741 aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg
    43801 aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta
    43861 accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac
    43921 aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca
    43981 tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca
    44041 ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac
    44101 gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag
    44161 atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta
    44221 aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa
    44281 tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg
    44341 gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc
    44401 aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta
    44461 ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat
    44521 acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac
    44581 cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt
    44641 tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg
    44701 tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg
    44761 gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa
    44821 atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa
    44881 ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa
    44941 acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag
    45001 agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat
    45061 tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca
    45121 gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa
    45181 gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat
    45241 tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt
    45301 tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct
    45361 ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt
    45421 gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg
    45481 acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct
    45541 cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt
    45601 gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa
    45661 ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct
    45721 gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt
    45781 ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca
    45841 tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg
    45901 ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc
    45961 attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca
    46021 acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa
    46081 atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca
    46141 gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga
    46201 gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa
    46261 gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa
    46321 taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca
    46381 ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa
    46441 atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga
    46501 aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa
    46561 gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga
    46621 ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa
    46681 agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc
    46741 accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt
    46801 tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg
    46861 tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag
    46921 tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt
    46981 tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt
    47041 taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc
    47101 aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga
    47161 atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt
    47221 cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac
    47281 atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac
    47341 ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta
    47401 tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag
    47461 aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg
    47521 gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg
    47581 acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac
    47641 tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct atttctatag
    47701 cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct
    47761 aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt
    47821 gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt
    47881 acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca
    47941 acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca
    48001 gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt
    48061 tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt
    48121 agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca
    48181 ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt
    48241 tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa
    48301 aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc
    48361 ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca
    48421 agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat
    48481 tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag
    48541 tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg
    48601 taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga
    48661 tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa
    48721 aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta
    48781 ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga
    48841 tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg
    48901 actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac
    48961 ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat
    49021 aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa
    49081 gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa
    49141 gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa
    49201 actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag
    49261 tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc
    49321 tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa
    49381 aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat
    49441 acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa
    49501 gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg
    49561 tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac
    49621 ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca
    49681 cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga
    49741 gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg
    49801 cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc
    49861 taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct
    49921 ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt
    49981 atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca
    50041 catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc
    50101 atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga
    50161 gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat
    50221 ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aagctatatt
    50281 tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct
    50341 gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac
    50401 ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca
    50461 ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc
    50521 gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt
    50581 tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca
    50641 agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac
    50701 gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt
    50761 agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa
    50821 gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta
    50881 aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg
    50941 cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa
    51001 ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa
    51061 acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca
    51121 tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa
    51181 acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa
    51241 gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt
    51301 acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt
    51361 ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa
    51421 tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca
    51481 tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa
    51541 tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt
    51601 cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta
    51661 agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat
    51721 acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac
    51781 aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac
    51841 aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg
    51901 tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa
    51961 cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact
    52021 cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac
    52081 gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat
    52141 acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga
    52201 aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa
    52261 gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca
    52321 gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat
    52381 gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt
    52441 cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc
    52501 gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa
    52561 gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg
    52621 ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc
    52681 gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg
    52741 gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg
    52801 agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg
    52861 gctattttca accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa
    52921 tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata
    52981 gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt
    53041 gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa
    53101 acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa
    53161 tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata
    53221 gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc
    53281 taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg
    53341 gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa
    53401 aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc
    53461 ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca
    53521 ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt
    53581 tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg
    53641 gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt
    53701 gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa
    53761 ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa
    53821 tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg
    53881 atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat
    53941 tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat
    54001 tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa
    54061 tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt
    54121 agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag
    54181 ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg
    54241 tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat
    54301 tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat
    54361 gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga
    54421 caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca
    54481 tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact
    54541 aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt
    54601 gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta
    54661 gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg
    54721 ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt
    54781 ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa
    54841 aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac
    54901 aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata tatatataca
    54961 ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa
    55021 agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa
    55081 cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg
    55141 taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct
    55201 tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa
    55261 cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca
    55321 ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga
    55381 ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt
    55441 atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat
    55501 agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga
    55561 aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc
    55621 tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc
    55681 tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat
    55741 tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat ttacggtata
    55801 caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg
    55861 acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt
    55921 tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca
    55981 agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac
    56041 gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg
    56101 agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc
    56161 cattccattt gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat
    56221 aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc
    56281 tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca
    56341 ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga agactttttt
    56401 caatattact catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca
    56461 agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac
    56521 gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga
    56581 agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa
    56641 agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt
    56701 attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga
    56761 tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca
    56821 ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta
    56881 tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga
    56941 gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca
    57001 atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc
    57061 aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg
    57121 gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga
    57181 tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac
    57241 gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc
    57301 tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta
    57361 gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac
    57421 gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt
    57481 caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg
    57541 gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg
    57601 attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa
    57661 gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc
    57721 attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca
    57781 gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac
    57841 gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata
    57901 cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt
    57961 ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta
    58021 ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa
    58081 ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat
    58141 attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg
    58201 catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa
    58261 gataataatc taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag
    58321 atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct
    58381 gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac
    58441 aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca
    58501 aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc
    58561 agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc
    58621 aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa
    58681 gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag
    58741 tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta
    58801 cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc
    58861 aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa
    58921 ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga
    58981 tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgct caaaatccgc
    59041 aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag
    59101 atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc
    59161 gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc
    59221 cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg
    59281 aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc
    59341 cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac
    59401 aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca
    59461 tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg
    59521 caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag
    59581 aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg
    59641 accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg
    59701 tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca
    59761 cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg
    59821 tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc
    59881 ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt
    59941 tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat
    60001 tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa
    60061 attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac
    60121 aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag
    60181 gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac
    60241 agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag
    60301 cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg
    60361 tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat
    60421 taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga
    60481 tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag
    60541 aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag
    60601 cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg
    60661 aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag
    60721 aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat
    60781 tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc
    60841 catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag
    60901 ctgatgatga gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg
    60961 tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg
    61021 atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca
    61081 tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct
    61141 caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa
    61201 aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa
    61261 ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc
    61321 actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat
    61381 gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa
    61441 acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa
    61501 aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca
    61561 gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc
    61621 atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat
    61681 atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg
    61741 aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag
    61801 ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg
    61861 cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat
    61921 cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc
    61981 atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt
    62041 ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac
    62101 aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag
    62161 gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag
    62221 ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt
    62281 tgctttgcgt gatgctttta tcggcacact gcccgcaaca atggcgggat cagtcgctgt
    62341 aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag
    62401 tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt
    62461 tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa
    62521 tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt
    62581 gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc
    62641 aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag atggcacact
    62701 gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac
    62761 agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat
    62821 tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt
    62881 accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc
    62941 tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt
    63001 atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt
    63061 tggtattcat ggatcaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca
    63121 actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt
    63181 gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt
    63241 tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc
    63301 taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat
    63361 caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt
    63421 cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt
    63481 cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc
    63541 aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt
    63601 gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca
    63661 ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag
    63721 gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt
    63781 tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc
    63841 tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa
    63901 tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg
    63961 tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt
    64021 ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg
    64081 tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag
    64141 aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc
    64201 gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa
    64261 atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa
    64321 ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc
    64381 gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg
    64441 tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat
    64501 gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat
    64561 gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag
    64621 taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt
    64681 tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt
    64741 gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga
    64801 gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat
    64861 cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac
    64921 ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc
    64981 aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat
    65041 ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa
    65101 atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata
    65161 aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg
    65221 gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa
    65281 aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc
    65341 gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat
    65401 gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa
    65461 tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct
    65521 gtaatgatca caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat
    65581 tatccattta aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg
    65641 aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa
    65701 gtagaacaaa caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta
    65761 ttagggtatc gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa
    65821 tatgaagaaa cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt
    65881 tgataaaaaa cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat
    65941 ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt
    66001 ttgttttagg gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat
    66061 tttttttaga tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg
    66121 gaaaggacct ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt
    66181 ggaaacgttt tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa
    66241 acaaacaagc tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat
    66301 cgcctcctaa gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg
    66361 tatcctttgc ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta
    66421 tcatttttat ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc
    66481 tctcgaacaa tatgaa
//
LOCUS       DBNIBO010000003        59472 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.003, whole genome shotgun sequence.
ACCESSION   DBNIBO010000003 DBNIBO010000000
VERSION     DBNIBO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 59472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.003"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(153..551)
                     /locus_tag="AC1Y77_000134"
     CDS             complement(153..551)
                     /locus_tag="AC1Y77_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIQ1573745.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="AC1Y77_000135"
     CDS             complement(548..1321)
                     /locus_tag="AC1Y77_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIQ1573746.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="AC1Y77_000136"
     CDS             complement(1318..2001)
                     /locus_tag="AC1Y77_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIQ1573747.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="AC1Y77_000137"
     CDS             complement(2168..3055)
                     /locus_tag="AC1Y77_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1573748.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="AC1Y77_000138"
     CDS             complement(3052..3921)
                     /locus_tag="AC1Y77_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1573749.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="AC1Y77_000139"
     CDS             complement(4058..5344)
                     /locus_tag="AC1Y77_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1573750.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="AC1Y77_000140"
     CDS             5617..6195
                     /locus_tag="AC1Y77_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1573751.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="AC1Y77_000141"
     CDS             6195..7124
                     /locus_tag="AC1Y77_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1573752.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="AC1Y77_000142"
     CDS             7121..8857
                     /locus_tag="AC1Y77_000142"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing sensor histidine kinase"
                     /protein_id="HIQ1573753.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="AC1Y77_000143"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="AC1Y77_000143"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIQ1573754.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="AC1Y77_000144"
     CDS             complement(9753..10262)
                     /locus_tag="AC1Y77_000144"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIQ1573755.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="AC1Y77_000145"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="AC1Y77_000145"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIQ1573756.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="AC1Y77_000146"
     CDS             complement(11563..12654)
                     /locus_tag="AC1Y77_000146"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIQ1573757.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="AC1Y77_000147"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="AC1Y77_000147"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIQ1573758.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="AC1Y77_000148"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="AC1Y77_000148"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIQ1573759.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="AC1Y77_000149"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="AC1Y77_000149"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIQ1573760.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="AC1Y77_000150"
     CDS             complement(16617..17591)
                     /locus_tag="AC1Y77_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HIQ1573761.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="AC1Y77_000151"
     CDS             complement(17572..19455)
                     /locus_tag="AC1Y77_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HIQ1573762.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="AC1Y77_000152"
     CDS             complement(19473..21698)
                     /locus_tag="AC1Y77_000152"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIQ1573763.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="AC1Y77_000153"
     CDS             21813..22847
                     /locus_tag="AC1Y77_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1573764.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="AC1Y77_000154"
     CDS             complement(22914..23927)
                     /locus_tag="AC1Y77_000154"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1573765.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="AC1Y77_000155"
     CDS             complement(24005..24193)
                     /locus_tag="AC1Y77_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIQ1573766.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24633..24761)
                     /locus_tag="AC1Y77_000156"
     CDS             complement(24633..24761)
                     /locus_tag="AC1Y77_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIQ1573767.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24764..26137)
                     /gene="rlmD"
                     /locus_tag="AC1Y77_000157"
     CDS             complement(24764..26137)
                     /gene="rlmD"
                     /locus_tag="AC1Y77_000157"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIQ1573768.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26248..27303)
                     /locus_tag="AC1Y77_000158"
     CDS             complement(26248..27303)
                     /locus_tag="AC1Y77_000158"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIQ1573769.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27313..28743)
                     /gene="gatB"
                     /locus_tag="AC1Y77_000159"
     CDS             complement(27313..28743)
                     /gene="gatB"
                     /locus_tag="AC1Y77_000159"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIQ1573770.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28740..30212)
                     /gene="gatA"
                     /locus_tag="AC1Y77_000160"
     CDS             complement(28740..30212)
                     /gene="gatA"
                     /locus_tag="AC1Y77_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIQ1573771.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30212..30517)
                     /gene="gatC"
                     /locus_tag="AC1Y77_000161"
     CDS             complement(30212..30517)
                     /gene="gatC"
                     /locus_tag="AC1Y77_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIQ1573772.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30623..32659)
                     /gene="ligA"
                     /locus_tag="AC1Y77_000162"
     CDS             complement(30623..32659)
                     /gene="ligA"
                     /locus_tag="AC1Y77_000162"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIQ1573773.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32676..34910)
                     /gene="pcrA"
                     /locus_tag="AC1Y77_000163"
     CDS             complement(32676..34910)
                     /gene="pcrA"
                     /locus_tag="AC1Y77_000163"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIQ1573774.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35065..36624
                     /locus_tag="AC1Y77_000164"
     CDS             35065..36624
                     /locus_tag="AC1Y77_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HIQ1573775.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36735..38666)
                     /locus_tag="AC1Y77_000165"
     CDS             complement(36735..38666)
                     /locus_tag="AC1Y77_000165"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIQ1573776.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(38684..39598)
                     /gene="pfkB"
                     /locus_tag="AC1Y77_000166"
     CDS             complement(38684..39598)
                     /gene="pfkB"
                     /locus_tag="AC1Y77_000166"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIQ1573777.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39595..40347)
                     /locus_tag="AC1Y77_000167"
     CDS             complement(39595..40347)
                     /locus_tag="AC1Y77_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1573778.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40506..40715)
                     /locus_tag="AC1Y77_000168"
     CDS             complement(40506..40715)
                     /locus_tag="AC1Y77_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573779.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40726..42054)
                     /locus_tag="AC1Y77_000169"
     CDS             complement(40726..42054)
                     /locus_tag="AC1Y77_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573780.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42190..42345
                     /locus_tag="AC1Y77_000170"
     CDS             42190..42345
                     /locus_tag="AC1Y77_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HIQ1573781.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42419..43006)
                     /gene="yihA"
                     /locus_tag="AC1Y77_000171"
     CDS             complement(42419..43006)
                     /gene="yihA"
                     /locus_tag="AC1Y77_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIQ1573782.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43089..44339)
                     /gene="clpX"
                     /locus_tag="AC1Y77_000172"
     CDS             complement(43089..44339)
                     /gene="clpX"
                     /locus_tag="AC1Y77_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIQ1573783.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44586..45875)
                     /gene="tig"
                     /locus_tag="AC1Y77_000173"
     CDS             complement(44586..45875)
                     /gene="tig"
                     /locus_tag="AC1Y77_000173"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIQ1573784.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46029..46961)
                     /locus_tag="AC1Y77_000174"
     CDS             complement(46029..46961)
                     /locus_tag="AC1Y77_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573785.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47118..47777)
                     /locus_tag="AC1Y77_000175"
     CDS             complement(47118..47777)
                     /locus_tag="AC1Y77_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIQ1573786.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47780..48805)
                     /gene="sppA"
                     /locus_tag="AC1Y77_000176"
     CDS             complement(47780..48805)
                     /gene="sppA"
                     /locus_tag="AC1Y77_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIQ1573787.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48818..49108)
                     /locus_tag="AC1Y77_000177"
     CDS             complement(48818..49108)
                     /locus_tag="AC1Y77_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573788.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49518..51323
                     /gene="glmS"
                     /locus_tag="AC1Y77_000178"
     CDS             49518..51323
                     /gene="glmS"
                     /locus_tag="AC1Y77_000178"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIQ1573789.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51395..52750)
                     /gene="glmM"
                     /locus_tag="AC1Y77_000179"
     CDS             complement(51395..52750)
                     /gene="glmM"
                     /locus_tag="AC1Y77_000179"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIQ1573790.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52774..53943)
                     /locus_tag="AC1Y77_000180"
     CDS             complement(52774..53943)
                     /locus_tag="AC1Y77_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIQ1573791.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53940..54827)
                     /gene="cdaA"
                     /locus_tag="AC1Y77_000181"
     CDS             complement(53940..54827)
                     /gene="cdaA"
                     /locus_tag="AC1Y77_000181"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIQ1573792.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55016..58696)
                     /gene="nifJ"
                     /locus_tag="AC1Y77_000182"
     CDS             complement(55016..58696)
                     /gene="nifJ"
                     /locus_tag="AC1Y77_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIQ1573793.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            58894..59415
                     /locus_tag="AC1Y77_000183"
     CDS             58894..59415
                     /locus_tag="AC1Y77_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIQ1573794.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        16485 a        12919 c        10664 g        19404 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaaaaa gactaagaaa caataacaaa
    24601 agttaacact tcttagtctt tctttagcac gtctatttat taaagatttt agggcctttt
    24661 ctcatgtttt tacgatcatt cttaggtgtt tccatttgtt tggatcggcc tccaccttgt
    24721 ttctttttga tcaattcttt cattttttct ttttggtcca tttttattct cctttaacga
    24781 atgaagcgac taattcgata tgatgggttt gcgggaaaag atctacaggc tgaactttct
    24841 tcacggcata acctgcttct gcaaataact tagcatctct tgcaaaagtt gccggattac
    24901 atgagatata gacgattcgt tctggtgaca tttcgattgc tgattcaata aaatgatgat
    24961 ctaatccttt tcgtggaggg tcaactacga tcacagatgg tttgatccct ttttcttgcc
    25021 attttttcat aactttttct gcttttccga cttcataatg aacattttcg attttattat
    25081 ttaaagcatt gaactgtgca tcttcaattg cttctggaat aacttccatc ccatacacct
    25141 tatccactct gtctgctaaa gacaagccaa tcgtacctat tcctgaataa gcgtcgatca
    25201 caatgtcttt tttatctaac tcggcaaact cgatggctgt ttggtacaga tactctgctt
    25261 gttgagtatt tacttgataa aatgactttg ctgagattcg ataggttttt cctaatagct
    25321 gatcttcgat atagtctctg ccatataaaa ctttttcttt gtctcctaaa atcacatttg
    25381 ttttttcttc attgatattt tggatgaccg aaaccacttc tggcaattct tcatgaatag
    25441 cttcagcaat ttccttggat ttaaagaatt tttctttacg agtaactaaa acgaccatca
    25501 tttcatggga ataatgacca cgacggataa tgatgtgacg aagctgtcct tcattttttt
    25561 cttcattata tcctcgtact tggaaccgtt gcaagatatc tctgactact ttgattgctt
    25621 gatcgatgac aggatcttgg atataaaaat cttcgatagg gactagatca tggctattct
    25681 ttttgtaaaa accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat
    25741 tacggtagcc gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct
    25801 tagcaatctt cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat
    25861 acttcaaatg ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct
    25921 gacgatcagg tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg
    25981 cttttactac tagtatttct acttcttctc cagggagtgc attttcgata aataatggat
    26041 aaccatcgat ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat
    26101 atcgttcgtt tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt
    26161 ttcaatccta ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac
    26221 cttactacgt tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat
    26281 ttttccgtca ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt
    26341 gctgacaacc tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc
    26401 ggtattggca aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc
    26461 gccgtattca ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac
    26521 gaacaaactg cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt
    26581 cagcatgagt gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa
    26641 agaaaacttc ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga
    26701 gttcgtcagc cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt
    26761 caagcgcatt ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc
    26821 aagataacca aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata
    26881 gccacctgca gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac
    26941 ggtttggtgt ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa
    27001 tgctcgagca tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc
    27061 aagcggagcg attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac
    27121 tagatcaaaa cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg
    27181 tgtagttgca taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc
    27241 caaatttttt ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt
    27301 cattttcagc ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg
    27361 atttgcttgt cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc
    27421 ttttccattt ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat
    27481 tggcagtagt ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg
    27541 ggcttctccg ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat
    27601 cgttccatcg gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc
    27661 agctaattcc agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga
    27721 tgcttgcttg gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt
    27781 tgtaagtgtc aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc
    27841 gcgacgagaa gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc
    27901 gatcacgaat ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt
    27961 gacacgcatc aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat
    28021 ttctcctcct gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt
    28081 cacgaaagat aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc
    28141 ataagggcga agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc
    28201 gctgacttct gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc
    28261 tggtgaacgc atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa
    28321 atcaacgtaa gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg
    28381 gacacgttcg atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc
    28441 atgaccgatt ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa
    28501 gtaatttttc cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc
    28561 tttcatcccg aattccagcg cagctttatt cattactggc agcacgcctg gataacccca
    28621 gtcgatcacg tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa
    28681 aatttttgag ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt
    28741 cattcgtctt tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca
    28801 aacgcatatg ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat
    28861 ccaactggta atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag
    28921 ttgacaggga tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca
    28981 ccgattccga atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag
    29041 actttttcaa aatctcgttt gatcaatgta cggacttgtc ccgctttttt gaagtaagcg
    29101 tcataataac cagcacttaa ggagaatgta cctaacatga tacggcgttt tacttcttca
    29161 ccgaatcctt ctgaacgtga gttgacatat acatcatcta atgatttcac attttctgag
    29221 cgataaccgt agcggatacc atcgaatcgt tgcaagttgg aacttgcttc tgatgaggca
    29281 atgatataat aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca
    29341 gcacctaatg aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc
    29401 acaccttcgc ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc
    29461 agtccctctg caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa
    29521 ccgctgatag ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt
    29581 tggtcaagag atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt
    29641 ttcatcccta cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta
    29701 cctaaagaaa ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca
    29761 ccaggtactt ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta
    29821 cttccaccca tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga
    29881 tagattttat ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat
    29941 gcagcggttg tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca
    30001 gcaaggacgt tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct
    30061 ttttcttcgt tcaatgtaat gaatgcttcg acctcaggct ctgtttcttt gattcgttca
    30121 aatgttgctt ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt
    30181 tcttctagag aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga
    30241 ttgcgggtac tttgataaag ccgtcttctg tttccggcac atttttcaat aattcatcgc
    30301 ggttccatcc agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg
    30361 acgtaaatgg aacaccttct gtatctactt cttccagcat ttcaaccata tcgatgattt
    30421 tacctaattg gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt
    30481 tggcaacgtg tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag
    30541 ttaaaaactt tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa
    30601 tttgtctgaa aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg
    30661 ccatttgatc ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc
    30721 cagcatcttc tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc
    30781 cgccaagatt ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag
    30841 taaggactac tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa
    30901 gccctttgta ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt
    30961 cttcgttact gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg
    31021 caataatcga ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta
    31081 aaatttttgc tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga
    31141 ccgaattatc tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta
    31201 ttttgtccaa cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta
    31261 ctagtttttt atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg
    31321 catttcttga aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga
    31381 tgcaccgtaa ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat
    31441 gtgtaggcaa ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa
    31501 cttctgggat gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct
    31561 tcaacttaat ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc
    31621 ggactggtgt catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga
    31681 tgttttcgat tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg
    31741 gtgctttgac agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga
    31801 ttccatcgat ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc
    31861 acacttcatc aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt
    31921 ctagctcttc tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg
    31981 tgtataaaaa agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag
    32041 aaccagctgc ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt
    32101 ggttcaattg gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca
    32161 ttggttcttt taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt
    32221 ctcctacagt tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca
    32281 aagaaacgga aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg
    32341 ctttttgtac acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac
    32401 tgtaaagcgg aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc
    32461 gttgagtggg agaatcagac gtgatcaaat caggatattg ttcctcaatt gctactaatt
    32521 ctgcgtattc tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt
    32581 cgcgagacca ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa
    32641 tcgtcatttc atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag
    32701 gcagccaaaa gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc
    32761 gacgtcccgc ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct
    32821 ccaggtttcc agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc
    32881 tctggctgtt tatagctata tgctgttttt ttagcgaata caggtgtttt tttaggcgta
    32941 ggacgcatgc cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt
    33001 cggttatatt gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct
    33061 tcttctgctc gcgtgatccc cacatatgcg agacggcgtt cttcttccag ttcgctttct
    33121 tccatcaatg ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact
    33181 gggaattcta atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct
    33241 tctaaattgt caatatcgga aacaagtgcc agatcattta agaagaccgt tagtttttct
    33301 tctggtgcat cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg
    33361 accgttaaaa attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt
    33421 tttaagtctt ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc
    33481 aagtatggga tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt
    33541 cctgcttttc caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat
    33601 tcatgaagag aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg
    33661 ggcgaattaa cgatacgctc aaaactgatt gaatcttgag gatttgccag cacattcaaa
    33721 tacgctaaaa tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta
    33781 taaggaatat ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg
    33841 tacaagatgg caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccga
    33901 tctacgataa aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt
    33961 ccttctttgt tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact
    34021 tggtttgctg cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact
    34081 gcagcatctg gataatcttt ttcaaaatcc aagatgttct gcatatctgc accgcgccag
    34141 ccgtaaatac tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat
    34201 aaattgacta gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa
    34261 tggaatttgt tttgataata tgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta
    34321 ttcatgatca ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca
    34381 gcataacatt ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga
    34441 ggggtctgca ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat
    34501 tttttcggat caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa
    34561 gaatcgatga ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata
    34621 cgcacacaca tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc
    34681 gcattgacac gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg
    34741 ttccaaggat tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc
    34801 gtctttccac ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct
    34861 tctttctgac gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc
    34921 ctttctttta tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt
    34981 tcaaaatcat ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa
    35041 attaaaagaa agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag
    35101 actcagatat attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt
    35161 agcctgtttc gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg
    35221 agtcgggaac agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt
    35281 ttcgtgggtt atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta
    35341 gagggacagc ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc
    35401 attggcggtg tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata
    35461 cagcttggcg catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa
    35521 gaaaagattt taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct
    35581 atcgcaggat tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg
    35641 ctgacatcat ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg
    35701 agacctgttt tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta
    35761 gtgatcttag gagtacttct aggcatctta ggttttattt atcagaaggt cttgctggct
    35821 ttgccaaaat tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc
    35881 ttgctgattt tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc
    35941 gttttagctt ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt
    36001 cgattcgttt tttccatgat ttcttacggt ggtggtcttc caggggggat tttccttccg
    36061 atattgacat tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg
    36121 atggatccaa tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg
    36181 attggaaaag caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat
    36241 catctgatgc ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc
    36301 ggtaatccaa tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca
    36361 ctaaaaggtg caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga
    36421 ttaatggtac gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga
    36481 aatcaggaaa tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc
    36541 ttgactgatg aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc
    36601 acaaatttga agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat
    36661 ctacactact tgattctata tgattctata aaaaaagaag agactgggaa aaaccagcct
    36721 cttctttttt atttctatgc ttctgctgct gcaggagctt ttctcaataa accataaatc
    36781 acaccagata cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt
    36841 aagaatacaa cgaaaatccc gccatgagga gccagtaatt tgatcccgaa agctccgact
    36901 aatccgccag ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct
    36961 gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa
    37021 ccagcatctt gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt
    37081 gccaatggag gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt
    37141 gcaacagtcg tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg
    37201 cctaaatctg ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca
    37261 ctcatacttg tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc
    37321 agcaacatca agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta
    37381 attccatcca atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa
    37441 ccagctaaga atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat
    37501 gcaccagcag caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat
    37561 cctgctaata ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta
    37621 gcagcttgat tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg
    37681 tcgatcatga aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt
    37741 gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct
    37801 tcttctgaac tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct
    37861 agattgatca gctcttctgg tttacggata ccatcactga caggacgatt gatcagttct
    37921 tttccgtcaa agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca
    37981 cgggcgatgt cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact
    38041 ttgatctcca cacccatttc tttcgctttt ttcttcagag catcttctgc catgtaagtg
    38101 tgagcgatcc ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca
    38161 gaagcttctg cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca
    38221 gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg
    38281 aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt
    38341 gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca
    38401 taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt
    38461 ttcgcgtgag gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct
    38521 agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg
    38581 accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct
    38641 ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga
    38701 tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag
    38761 ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc
    38821 tgtaagcccc aataaagccg gcaatcattg aatcccctgc acctactgaa tttttcaatg
    38881 ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc
    38941 cagccattga gatcagcaca ttctgtgccc cttcagctag caatttttta ccatacggaa
    39001 tgatttcttc tacagattgg aaggttgtat tgtaaagctc agctaattcg tgattgtttg
    39061 gtttgatcaa caatggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa
    39121 tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc
    39181 gcaggctagc cggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta
    39241 atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcccaaa ccgttaattt
    39301 cagtttcagt atctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga
    39361 acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc
    39421 ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga
    39481 tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta
    39541 acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg
    39601 tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg
    39661 ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa
    39721 tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac
    39781 agctgcctct tctgggtccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc
    39841 cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac
    39901 agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac
    39961 taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa
    40021 aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat
    40081 ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc
    40141 actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc
    40201 tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt
    40261 aaaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat
    40321 tttttgtcta cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata
    40381 ttttttttct actcgcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc
    40441 aaaaaaaaat aaaaaaccag tcaaaaagac tggtttttta tttttttgtc tatcatcaag
    40501 ccgttttatc gttttttatt ttcgcttctt gtttttttca tataaacaga agcaggtttt
    40561 ctttccttat gaagaagata ataataaagt cccatcccgc caaacatgac caaaaaaccg
    40621 atgactgatc gaataggaaa attaggaaca aaaaagagac agacggataa taatgatgcc
    40681 ggaaccatta ttcgataata gagttgttta tccattttct tcacctcata ttttttgtaa
    40741 agtaatcaat tgttctaatt ctcggtaatc taaaaattgc acacgcagat gctgctcacc
    40801 tttcttgaca tcgaattgga tacttcgccg ctttttcttt attcgtttga aggtaacatc
    40861 agagagtcta tgtttgattt cctttaacgg taattctgac agggattctg aaggaacata
    40921 gattcttaag gataaaagat actcttgata tttttcatct gttacctcta cttctagata
    40981 ttccctaata taataacaat aagcttcgat tccattatcc aaagcaacag tcggatcgat
    41041 aactttatag cacatcatgc gcgagcgatt ctcgtcaacc caaggaaaat acgcctgagc
    41101 atttagtgtt ccgcgaacac gcctttcttt ataatctcct tgttgaatat cataactttt
    41161 tgttacaaaa aatgaagcat tctttttagg aacaggttgc atttgtgcat tctgatactg
    41221 gacagattgg atcgtatggt cagtgtaaat gtattcaacg aatggcggtt tcaataaacc
    41281 gacaccattg tcccaagcaa gatgatcaaa cccaagctgg tccctaaaat aggattgaaa
    41341 catttgattg tttggctttt gccagttaaa ggggtctcga taaagctgga tacatatgac
    41401 ttcgttttct aattccttca ctttttctac ttgttggatg tcgagaaaaa tcggtttagg
    41461 gatgatttga tgataatagc gactataata catttcccaa cacgaagtga aacataaacc
    41521 ttggtaaaaa agatcgtatc ctggaaattg gttgcaatcg actactactt ccccgttttg
    41581 ttgtttcatt ttaggtaaat gttcgatttc atcctttttc tcaaacaata gccgttgaga
    41641 gatctgtttt gtattgtgat agataaactc gctataagct cgcatatagg cgaacacccc
    41701 atgagcaatc atttttttct gaatgaagtc tcgaatcaaa aattgctttt ctttgaaaac
    41761 agagatagac aatacatttt ttatcaataa ttgattatct tccaagcgaa aactacattc
    41821 gttctcgtca tttcctacaa ctaaccaact ttcgtctctt accgatgcta gcattatttt
    41881 ttcttgaagc tttcgaagat catacggatc cgcttgttgt gtgaaagagc ggataaatgt
    41941 tggctggatc aacagacgtg tcaaaatagc ccaatcgctt atttcctgtt ttttttcttc
    42001 ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa tcattttctc
    42061 acctctttaa aatcttttta taatttaatt ataaccaacg ataaagaaaa actaaaactt
    42121 tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag aaacaaagag
    42181 gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact tttcgaatca
    42241 tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg atttctaaaa
    42301 ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa gctcaaaaaa
    42361 agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt tattcttttt
    42421 atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt tttgtaacgg
    42481 aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg atttcgctct
    42541 catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca acaattacag
    42601 ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa ggcttatgac
    42661 gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa tatgtctcga
    42721 tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca tatcccggaa
    42781 catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg gtctttcctg
    42841 gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata aaagaagatt
    42901 tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct ggatattgtt
    42961 ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc tctctcctta
    43021 acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt tacggcagcc
    43081 tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg ctcaccagac
    43141 aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga agggatatca
    43201 aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc accggtattg
    43261 cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc aagttcggta
    43321 ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg ctcagtcaag
    43381 atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg taatcgtccg
    43441 ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg ctgcatgaca
    43501 ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt ttcgcccata
    43561 cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa caatatgttc
    43621 gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtcctccctg aggtggcacg
    43681 cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc gccggaaacg
    43741 tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc atcaatatag
    43801 atgatccctt tttctgcacg ttccacattg taatcagcag attgaagcaa tttaagcaaa
    43861 atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc atctgcgatt
    43921 gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt accagaccct
    43981 gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt gttttcttgt
    44041 gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga gcgttttgcg
    44101 cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg tttagggact
    44161 tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc tttacatagg
    44221 tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt cacttcttct
    44281 tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt gtcatacatg
    44341 gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt tacagtcttt
    44401 tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc aactgaccta
    44461 tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat cgtgtcacag
    44521 tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga tttattactt
    44581 gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc ttttttcatt
    44641 gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc gattggcatg
    44701 ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt tgcttgaaga
    44761 ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat ttctgcttca
    44821 ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata gtacatttca
    44881 ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg atgtacttca
    44941 tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc catacggatc
    45001 gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa ttctttgcga
    45061 tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt tgcgaattcg
    45121 tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt gaagactgct
    45181 tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt tacatctaga
    45241 ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa tgagcctgaa
    45301 cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc atctacaaag
    45361 ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc tttgataact
    45421 aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc atctgttact
    45481 tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac ttctggtttc
    45541 actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc aacatcgatt
    45601 tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata agcttctggt
    45661 aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg gttgaatact
    45721 tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt cactttatta
    45781 aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt taaaacacca
    45841 tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag tttagaaatt
    45901 cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa ccctttcaaa
    45961 ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat ctttttcact
    46021 gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt ttttaaaaaa
    46081 tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc ctctacagtc
    46141 agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata aacaattgca
    46201 gattgcaacg tgaattcttc atttaacacg gaactaagca gcgcatcttc attaccgtaa
    46261 cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt tggcagatct
    46321 tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg atggcattcg
    46381 ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac ctctggcgat
    46441 acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc gatctcatca
    46501 atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt attgtggagt
    46561 gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg gattttttca
    46621 atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt tctagcatat
    46681 aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc ctcattttga
    46741 agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc gtccaactcg
    46801 taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc gtaagctcgt
    46861 tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg tgctactcgc
    46921 attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc tcctcttgct
    46981 catgttcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt aaaaataact
    47041 gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt tcaactgtta
    47101 ctgcttttta ttgctcatca gctgttgact tcctggttga atgctttgat cagattgatc
    47161 gtaacaacac ttgtattagt gaaaaaatcg cctagatgtt gctttttctg attaaagatt
    47221 gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca agcaccttct
    47281 ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac tcgaatacca
    47341 aagatcattt tcccaatcgt ttgaccatga ttgaatttcg tcaataaaat aaaatacgct
    47401 agataaataa gtaaggctgt gaatccgtag atactcagat aagaatctga aaaattaagc
    47461 caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat acacaaaaga
    47521 tcgaccaaga aagcaaacat tcggataaaa aaacctgcat agaaatactt aggaaaatca
    47581 tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg ttgtttcttt
    47641 ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt ttgggaaaag
    47701 ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc cttattttca
    47761 tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt ggtgattccg
    47821 ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc ttcaatccct
    47881 ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc gtggaagaat
    47941 attctactag ttccgcatct tcaagctgtt gttcctttcg catagctttt aaagcatctt
    48001 caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca tcataaatcc
    48061 ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg ctgacgattg
    48121 ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct tcttcttttg
    48181 tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact gttgtatctt
    48241 ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc acaccaattg
    48301 agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa atatagtagc
    48361 caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt tctttacgaa
    48421 tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca ggagaattta
    48481 cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt tgttccataa
    48541 aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc gcagcaatcg
    48601 ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc aacacatttg
    48661 aagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga tcgttttcct
    48721 gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca gcaatcagaa
    48781 ctgctgccca acgtctctta ttcattcttt ctcctttcta atacaagttt tttatgacga
    48841 tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc cgcgcatacc
    48901 cggaaaacga ctcttttgtc aattcatctt ctaccgtaaa ctcacgagct tcagcaactt
    48961 ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa aatattttcg
    49021 tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt aacattccca
    49081 gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat tactttctat
    49141 tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga cataaaaaga
    49201 gcatttgaca aagcatttga tatataccaa tttatttttt attctttttt tgttgacaag
    49261 aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa gaggaggata
    49321 gttatacctt ttaggatagc gccaggtctg tgtaaacaga tgacgaggag agggtttatc
    49381 gaaagattcg gcggatggcc ctagggactg cactctacag aaaatccaca aaaaatatct
    49441 gcaaagataa aacaaaagga ttttcctgca gctggaagca ttcatacgaa ttcattatag
    49501 aaagtaggat gttttttatg tgcggaattg ttggaatgat tggattaaaa aatgtaacac
    49561 caggattgat tgacggtcta gaaaaattag aataccgtgg ttatgattca gctggtatct
    49621 ttgtctcaga tggcacaact gattatctgg tcaaagcaca aggcagaatt caaaatttga
    49681 aaaacaaaat aaatattgat actacaggaa atataggtat tggtcataca cgctgggcaa
    49741 ctcacgggca accttctgaa gaaaacgccc atccacatac ttcccaaagt ggacgcttcg
    49801 tgctcgttca caatggcgta atcgaaaact ttgaagaact taaaattgct tatttagcaa
    49861 atgaccactt tattggtgag accgatactg agattatcgc tcatctaatt gaaactttcg
    49921 cgaaggatac tacaacgcaa gaggcgtttt taaaagcttt gcgggtcatc aaaggttctt
    49981 acgctttcgc tttgattgat cggacagcac ctgatgttat ttacgtcgct aaaaataaaa
    50041 gtccactatt aatcggactc ggcgatggat tcaatgtgat tgccagcgat gcaatggcta
    50101 tgcttgctca tacgaaggaa ttcgttgaga ttgaagatga agagatggtt accgtcacat
    50161 cagaaaaagt tattattcaa aattttgcag gtgacatcgt agaacgttct tcattcgaag
    50221 cacaagtaga ttcttcagat atcgagaaag gtacctatcc tttttatatg ctgaaagaaa
    50281 tcgacgaaca gcctattatc atgcgtcgta tcgctcaaaa atacgtaaca gaagacaatc
    50341 gtgtctctct tgatcaaaag ctggtcgata cattgtctga tagcgatcga atctatatcg
    50401 ttgcttgcgg aactagttac catgcggggt tagcaggtaa acagactctg gaaaaattga
    50461 cacagatccc tgttgaggtt catttagcca gtgaatttgg ctataacaca cctcttcttt
    50521 caaaaaaacc attctttatt ttcttgagtc aaagtggcga aacagcagat agccgccaag
    50581 ttctggtaaa aatcaaccgt ctcggctacc cttcacttac gattacaaat gttgctggtt
    50641 ctactctttc gcgggaagca tcctttacac tgctattgca tgctggaccc gaaatcgcag
    50701 tagcatcaac gaaagcttat actgcacaaa tagctgtact aactttatta gccaaagcta
    50761 tcggtgataa aaaagaattt gaaacttctc ttgtattcga tatcactcat gaattgagtt
    50821 tagttgctaa tgcgatggaa tcggtcatcg cccaaaaaga ttatttagaa gagttggcag
    50881 aagtatatct cagcgataca agaaatgcct tttacattgg acgtggcgct gactacaacg
    50941 tttctcttga agctgctttg aaattgaaag aaatcagtta tatccaggca gaaggttttg
    51001 ccgctggcga attgaaacat ggaacgattg ctttgatcga agaaggaaca ccagtgattg
    51061 gaatcatcag tgaagaagtg actggtgcgc atactcgagg aaacctgaaa gaagtcgaaa
    51121 gccgtggcgc taaaacacta gttattgtct cagagggatt agaaaaggaa agtgaccagc
    51181 tggtgctacc aactgtccac ccttatctaa ctactctaac cacagtcgta ccgactcaat
    51241 tacttgcata ttacgcaacg ctattacggg gattcgacgt tgacaaacca cgtaatttag
    51301 cgaaatcagt gacagtagaa taataaaaga acaaggacca gtgtcagaca tctgacactg
    51361 gtccttgttc ttttattttt tatgaaaata tctcttaatc cagtccgatt tcttctttga
    51421 ctactgccgc gattttatcg acatagtaat taactttctc atcagtaggc gcttctgcca
    51481 tcactcgaag taatggttct gtaccggaag gacgaactag gattcttcct tctgatccca
    51541 tttcagcttc tgcctcttca atcacttttt tgattgccgg aacatccatc gcaccgtttt
    51601 tatcacttac tcggatattc actaattttt gcggatagat tgttacctct gctgcaagct
    51661 ctgataattt cttacctgtc tgtttcatga tattcagtaa ctgaattcct gaaagcatcc
    51721 catctccagt tgtgttaaaa tctaagaaaa tcatgtgccc agactgctcg ccgccaaagt
    51781 tataatcgtt tttgcgcatt tcttctacaa cgtaacgatc accaacttgt gtaacgacat
    51841 ctttcaaacc gattccttcc accgctttgt ggaatcctaa gttactcatg acagtcgtaa
    51901 cgatcgtatc ttttttcaat cgattttttt ctgccaagta tttagcacag ataaacatga
    51961 ttttatcacc gtcgacaatg tttccaagtt catccaccgc aataatacga tcgccatcgc
    52021 catcgaaagc tagtccagca tctgctcctt tttcaacgac catctcagct aaacgttctg
    52081 gatgggtaga gcctacgcca tcattgatat ttaacccgtc tggatttgtg cccatggtat
    52141 aaaaatctgt ttcaagatcg gcaaacaaac gattaacact tgttgaagtt gctccgtttg
    52201 ctgcatcgac acaaatggtc aaaccggaaa ggtcaccgtt gatcgtttgt acaaggaatt
    52261 gagaatattt caataagcct tctggaaatt cttctacagt tcccaatcct tcagctgatg
    52321 gacgaggcaa ttcatccact tcagcatcta ataatgcttc aatttctagc tcttggtcat
    52381 ctactagttt aaaaccatct gcaccgaaaa atttgattcc attatcttgt gctggattat
    52441 gagaagcaga aatcatgacc cctgcgcttg ctttttgcaa acgtgtcaga taagcaacac
    52501 ctggtgtaga gatcacacct agctggaaca cttcgatacc caccgataaa agtcctgaaa
    52561 tcagcgcttc ttctaacatt tgacctgaga ttcttgtatc ccgccctact aacacgcgtg
    52621 ggcgttttcc ttcttcttca tgctggctta ggacataacc tccgcaccgt cccagcttaa
    52681 atgcaagttc tggcgtaagt tccttgttgg cttcaccgcg tacgccgtct gttccaaaat
    52741 atttacccat agttgtaaaa ttactccttt aacttaattt tctgaggaat ttgttctgct
    52801 catttgtgtt gattcgcttg tttctgatgt ttcttctgta cttgtggtcg agctgcttgt
    52861 cccagattcc gaactggaag aaatggttgt tgtcgatggt tccgttcctg aatcagcaga
    52921 tgaaatactt ctactcgtac ttgtattttc ttgtgtccga ctgctcgtcg aagcagagct
    52981 actgcttgac gtttgcaaca cgggtgtaat ctccacacta actgattcgg gcttgatact
    53041 taacccctct tctactggta tttcaatttt acgagttgtt cgatgattga tacctgagac
    53101 attgatcggt acagcaatac tgtcaccgat cgatgcaagc aaatcgctag agccgcttac
    53161 ttctgcttcc agttttgaaa aactgaaatc atagctttct accccggcag ctggtgttcc
    53221 ttgctgtatc ccataaagtc gtaccgactt gcttggcttg atcgaatcag ctctcactgt
    53281 tacttgcact ggatcagaga cgatacttaa tacttcccct gctgcattca gcgcctgtat
    53341 tgggacctca ctattaatcc catcatctgt gaccatcgaa gcgtccacat cagctactac
    53401 tcgtttgatt tctgccaatg ttttgtcacc tgtagtgata gagacttttt taggactaac
    53461 ggaagtatca tcaagttcat atccagatgg tgtgaacgtt gaaggaatca acgtttccac
    53521 atcgaaatct tttattacct ttttctcaac cgttactgta atagtcgatg gttcgatctt
    53581 tgctgtgaca gcactgctca aattttgaat ttttagcgga acctcatgtg ttccttcgcc
    53641 cagcttagtg atatctgctg tgacgcggaa catccgtgta tcttcgtttg cctctgcatt
    53701 caactgaact cggtttgcac tggaaagttt gacagtcact gcattttcat atccatggat
    53761 atagtagcgc tttgaatcat aagaaatctt gattggcacc ccagtaactg tttgattata
    53821 cgcttcattt ccagaaagcg tatttggaac ggactggcca tttgcattga agtacagcgc
    53881 aatactgaac agcaatgcaa taaaactgta aagaatgctt gtccgtttct cttttgagat
    53941 catttgcgat cccctccctt agtcataccg tctaggaaag attgaatcac gtttttcttg
    54001 ttttttgttt tctcttcagc aatcagttct ctacgcaaaa ttgccaaata ttcttgctgg
    54061 gataagccag ccatcagttc attatctaat gtaatgctga catccccagt ttcttcagag
    54121 acaacgatgg taatcgcgtc gctgacttcg cttataccga cagctgcccg gtgtcgagtc
    54181 ccgaattctt ttggaatcaa catactttct gatagaggaa gataggcgct tgctactgcg
    54241 atctttcctt cttttacaat aactgcaccg tcatgcagcg gtgtattagg aataaagata
    54301 ttgatcagca gctctccagt gatatctgca tctaatgcaa ttcctgtctc aatatactca
    54361 tctaatccag tatgccgttc gatagtaatt agcgcaccga tttttcgctt ggacatatat
    54421 tggatggctt tgtcaaaaga caagatcatc ttctcatctt cctgctgttc gctttttgat
    54481 tgtttgaaaa gcgaactgcg tccaagatgt tccagtcctc tccggatttc cggctgaaaa
    54541 atcacgatag cagctatcac cccgtacgta atgacttggt tcatcagcca agacaaggtg
    54601 tgcagaccaa tgatttccgc tacgattcgg ataacaataa aaatagctac acctttcaga
    54661 agctgaatcg cttttgtgcc tcgcagcagt tggatcagtt tgtatacaag ataccagaca
    54721 accagaatat caagtatgtt caccaaaaaa ttcagagaga acaagtcacc ggaaaacatt
    54781 tgctgccaat agtccaaatt aaaaagttcc ccgatttgaa atgacataaa aagcctctct
    54841 tctcttttga gatttcctct ctagtatatc atatgtttta attttagagg ctgatctgct
    54901 cgtatttttc ttgagaaaaa cgcataattt tcaggaaaat tgcaagtatg cgttttattt
    54961 ttctctgcaa taaatatttt taaacttcag agaattattc attatatttt ttctgttatt
    55021 tttccttttt ttcttttgta gcttgttcca tttttttacg tcgttctgct ctggcagctc
    55081 ttctttcttc tgcttcaagg tccgtcgttt tttcttttct agtcttaacg ggtacatcga
    55141 tagattcctc tggttcaagt cgtttcttga ttttttctaa ttctcccgtc agtgcagcat
    55201 agttgtaaaa acgtgtttta gcgtcttgga tagtcttttc aaataaagct tttgcaagat
    55261 ttggatttgc gttctctagc gaggaaaagc gaacttgttt cctcatgaaa tcaggcatag
    55321 cagagaaatc aggttttttg tagtccaaag tcattgggtt cttgtttttt tcgaccattg
    55381 cagggttata tcgatataat gaccaatagc cagaagaaac agcttctttt gcttctttaa
    55441 gcgattcttt cattccacca agcaaaccat gtgtgataca aggcgtatag gcaatgatta
    55501 aagaaggtcc aggatacttt tctgcttctt caaaagcctt tatcgtctgc atttgattgg
    55561 cacctgaagc aatttgcgcc acataaacat ttccatatgt catcgccatc attcctagat
    55621 ctttctttga agcatacttt ccactagccg aaaatttagc gattgccgaa gctggtgttg
    55681 cttttgaggt ttgaccgcca gtattggaat agacttcgtt atccaaaaca agaatattca
    55741 cgtctgctcc gctggcaagt acatgatcga tcccgccaaa accaatgtcg taagcccatc
    55801 cgtctccacc aatcatccat tggctgtttt ttacgaatag atcctttctc ttgtatatat
    55861 cctccagttc agggacaatt gatcgttcgc tttcgatcgc agcttgcagt tttgctgctc
    55921 tttgctgcgt tccttcgctc tcaaacatgt gtaccaacca gtcttccatc aattctcgta
    55981 gttcttctga tacttttggc aatacttctt tgattgaact tgccaaagcc tcgcgacgag
    56041 tctgtgccgc tagaagcata ccataaccaa attcagcatt atcttccaat aaagaatttg
    56101 accatgcagg cccttgacca cattcattgg tcgtataagg agaagcagga gaagcaccgc
    56161 cccaaataga agaacagcct gtcgcattcg cgatcatcat tcgatcaccg aacatttgtg
    56221 tcaataattt tacataaggt gtttcgccac agcctgagca agcgcccgag aattcaagaa
    56281 gcggttgatt gaattgtgta gacaagacag tattctttcc tttgactgga tttgcttttt
    56341 gtcttaatgt cattgcaaaa gcccaattca cagcttgttc tttctgttct tcataaggct
    56401 tcatcactaa agctttttct tttgcgggac aagcctctac acacagacca caacctgtac
    56461 agtcttccac tgaaacttgg atacggtatt ttacgccgtc tgcgcctcgc atttctctca
    56521 cgataaatcc ttctggcgct tcttccattt cctcttcatc tgctaaaaat ggacggatgg
    56581 cagcatgagg acaaacaaag gcacattcat tacacatcgt acagcgatca ctaatccact
    56641 caggtacttc taaagcgacg ccccgctttt ccatagaagc cgttcctaaa ggcatttcgc
    56701 cagttgtcat tccatttgct acaagtgtac caacagagag ttgatttcct tcctgtgcat
    56761 tcactggttc aagtatttct tgtatataac cagtgttcct tttattgatt ttttcaggca
    56821 tctccaacgt tctccaatgg tctgggatcg cgacttgatg aagcagttcg gtcgtccgat
    56881 cgattgcttg tatgtttttt tctacgatag ccattgattt atgtccatag cttttcaggg
    56941 cttcttcttt tagcagcgga agcacctcat caaaaggaat gatgtcagct aatttaaaaa
    57001 aagccgtttc cattgccgta ttgatccggc gtcccaaacc gacttcttgg gccaatctca
    57061 tggcattgat cgtatagaat ctaatgttat tttcagccaa gtaacgtttt aattttaagg
    57121 gcagatgcgt ttctaattgt tcgtcgctcc aaagagtatt caataaaaaa atgccacctg
    57181 gcttcaatcc tttgaccaag tcatagctat gcaggtaagc tggtgtgtga caggcaacaa
    57241 ggtccgcatg ctcgacaaga tacgctgaac gtatcggcgt atctccaaaa cgaagatgag
    57301 agacagtcag accgccagat tttttcgaat cataataaaa atagccttgc gcatatttat
    57361 cggtgtgatc tccaataatc ttgatagctg atttatttgc tccaactgtt ccgtcagatc
    57421 caaatcccca aaacttggcc tggaatgttt gcggttctgt caaatccaga ctttcctttg
    57481 gttccaaaga ttgataagtc acatcatcca cgatccctat ggtaaaccgt tttttgcgta
    57541 tgcttgggtc ttttttcaat tcatcaaaaa ctgctgagat ttggtcggga gtcacatctt
    57601 tagaccccaa tccgtagcga ccaccaataa cagccgggcg aagttcagaa tcatataacg
    57661 cactttgaac gtctaataag agaggttctc cacctgcccc tggttcttta cttcgatcaa
    57721 gaacagcaat tgtttttact gtatgaggca acttttgcac gaatgtttcc actggaaaag
    57781 gacgataaag atggatattc aaaaaacctg ttttccgtcc gtttgcattc aagtaatcaa
    57841 tcgtttgttc aatcgtctgt gcaactgatc ccatagaaac aatgacttct tccgcatcgt
    57901 ctgctccgta ataagtcacc aagtcataag aagttcctcg taatcggttg atctctttca
    57961 tatatttttg aacgatttca ggtaatttat cataataggg gttgatcgtt tcccgttgtt
    58021 gaaaatggat gtctggattc tggttcgtac cactaacagt cggatgattg ggattcatcc
    58081 ctcgtttacg aaatgaagct aatttctcct gattgatcag aggagctaat tcttcatatt
    58141 ccaaaacttc gattttttga atctcgtgac tcgtacggaa tccatcaaag aaattcaaaa
    58201 aaggtacttc tccttcaata gaagcaagat gggcaacagg tgctaaatcc atcacttctt
    58261 gtacactgct ttcagctaac atagcaaatc ctgtttgtct tgcagccatc acgtctccgt
    58321 gatctccgaa aatattcaac gcatttgtcg tcactgcccg actagcaaca tggaaaacac
    58381 ttggcagtag ttccccagca atcttgtaca tattcggtat catcaaaagc agcccttgag
    58441 aagcggtata tgtagttgtc agtgcaccag ttttcaatga tccatggacc acaccagctg
    58501 ctcctgcttc ggattgcatt tctactactt ttactggttg accgaataca ttttcttttc
    58561 ctgctgctga ccattgatct actaattcag ccatggtcga actgggtgtt attggatata
    58621 ttgcagcaat ctcggtaaat gcataagaaa tatatgcggc tgccgtattg ccatccattg
    58681 ttttcatttt tcgcatgatt catcatcctt gttcatattc tctcttatta tctgttccat
    58741 tataacgtaa aacattccat tttttctttt atttataaaa atatttcaca tatgtctatt
    58801 tgaattggtc atctaatttt tagacaaaca gtctaaatcg ttgccttttt ttgtcagaca
    58861 tgtataaact ataagcgaaa gaggtgtttt aacatggata tatcagtgat tgatgcaacg
    58921 aaaacaaata cacaaaaagg aatcctttat ggttcatcga atgctcctaa aaaaatggtc
    58981 gaattcatca acctatcttg tccgtattgc cgacaatggt ttgaagaatc ttatgagcta
    59041 ttagaagaag cagtccaaag cgggcaattg cagcgagtga tcaaattgtt cgataaagaa
    59101 aaagaaagct tgcagcgagg aaacgtgatg caccggtatc tcacaatcag cgatggtcaa
    59161 aaagcgatca aagagatcaa acaaatattc gatactcaag acgagtggaa acatctttct
    59221 ctccaagaag tagcggattt tgcagtagat aaactaaagc tgacagaaca aaaagacgaa
    59281 cagctgagcc aagctgtcat caacgaagct gagcaagcac atattcgatt tgttccgact
    59341 gtcatcttgg gtaaagaaat ctttgatgaa tctatttcaa tagaggaatt aaaagaattg
    59401 atccaaacaa aataaaaaaa taaggagcgt aggacaaaag taaagaacac ttttgtctca
    59461 cgctctaaac ac
//
LOCUS       DBNIBO010000004        56347 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.004, whole genome shotgun sequence.
ACCESSION   DBNIBO010000004 DBNIBO010000000
VERSION     DBNIBO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.004"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            290..1135
                     /locus_tag="AC1Y77_000184"
     CDS             290..1135
                     /locus_tag="AC1Y77_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1573795.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="AC1Y77_000185"
     CDS             1416..2636
                     /locus_tag="AC1Y77_000185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIQ1573796.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="AC1Y77_000186"
     CDS             2633..5353
                     /locus_tag="AC1Y77_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1573797.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="AC1Y77_000187"
     CDS             5337..6281
                     /locus_tag="AC1Y77_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIQ1573798.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="AC1Y77_000188"
     CDS             complement(6350..7360)
                     /locus_tag="AC1Y77_000188"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1573799.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="AC1Y77_000189"
     CDS             complement(7540..7884)
                     /locus_tag="AC1Y77_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIQ1573800.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="AC1Y77_000190"
     CDS             complement(7877..8311)
                     /locus_tag="AC1Y77_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIQ1573801.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="AC1Y77_000191"
     CDS             8449..9186
                     /locus_tag="AC1Y77_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573802.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="AC1Y77_000192"
     CDS             9179..10390
                     /locus_tag="AC1Y77_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1573803.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="AC1Y77_000193"
     CDS             10594..11376
                     /locus_tag="AC1Y77_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIQ1573804.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="AC1Y77_000194"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="AC1Y77_000194"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIQ1573805.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="AC1Y77_000195"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="AC1Y77_000195"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIQ1573806.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="comGA"
                     /locus_tag="AC1Y77_000196"
     CDS             14092..15021
                     /gene="comGA"
                     /locus_tag="AC1Y77_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HIQ1573807.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /gene="comGB"
                     /locus_tag="AC1Y77_000197"
     CDS             14978..16027
                     /gene="comGB"
                     /locus_tag="AC1Y77_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HIQ1573808.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /gene="comGC"
                     /locus_tag="AC1Y77_000198"
     CDS             16024..16302
                     /gene="comGC"
                     /locus_tag="AC1Y77_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HIQ1573809.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /gene="comGD"
                     /locus_tag="AC1Y77_000199"
     CDS             16277..16747
                     /gene="comGD"
                     /locus_tag="AC1Y77_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HIQ1573810.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16719..17066
                     /locus_tag="AC1Y77_000200"
     CDS             16719..17066
                     /locus_tag="AC1Y77_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573811.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17029..17454
                     /gene="comGF"
                     /locus_tag="AC1Y77_000201"
     CDS             17029..17454
                     /gene="comGF"
                     /locus_tag="AC1Y77_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HIQ1573812.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /gene="comGG"
                     /locus_tag="AC1Y77_000202"
     CDS             17426..17785
                     /gene="comGG"
                     /locus_tag="AC1Y77_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HIQ1573813.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="AC1Y77_000203"
     CDS             17957..18964
                     /locus_tag="AC1Y77_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1573814.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="AC1Y77_000204"
     CDS             18989..20173
                     /locus_tag="AC1Y77_000204"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIQ1573815.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="AC1Y77_000205"
     CDS             complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="AC1Y77_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIQ1573816.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22298..22426)
                     /locus_tag="AC1Y77_000206"
     CDS             complement(22298..22426)
                     /locus_tag="AC1Y77_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573817.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22771..22914)
                     /locus_tag="AC1Y77_000207"
     CDS             complement(22771..22914)
                     /locus_tag="AC1Y77_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIQ1573818.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="AC1Y77_000208"
     CDS             complement(23137..23616)
                     /locus_tag="AC1Y77_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1573819.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="AC1Y77_000209"
     CDS             complement(23689..23973)
                     /locus_tag="AC1Y77_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573820.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="AC1Y77_000210"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC1Y77_000210"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="AC1Y77_000211"
     CDS             complement(24317..25603)
                     /locus_tag="AC1Y77_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIQ1573821.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="AC1Y77_000212"
     CDS             25741..26412
                     /locus_tag="AC1Y77_000212"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIQ1573822.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="AC1Y77_000213"
     CDS             26378..26866
                     /locus_tag="AC1Y77_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HIQ1573823.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="AC1Y77_000214"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="AC1Y77_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIQ1573824.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="AC1Y77_000215"
     CDS             27827..28591
                     /locus_tag="AC1Y77_000215"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIQ1573825.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="AC1Y77_000216"
     CDS             28824..31037
                     /locus_tag="AC1Y77_000216"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIQ1573826.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /gene="dtd"
                     /locus_tag="AC1Y77_000217"
     CDS             31048..31494
                     /gene="dtd"
                     /locus_tag="AC1Y77_000217"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIQ1573827.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="AC1Y77_000218"
     CDS             31665..32924
                     /locus_tag="AC1Y77_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIQ1573828.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="AC1Y77_000219"
     CDS             complement(33013..33960)
                     /locus_tag="AC1Y77_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIQ1573829.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /gene="hisS"
                     /locus_tag="AC1Y77_000220"
     CDS             34354..35646
                     /gene="hisS"
                     /locus_tag="AC1Y77_000220"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIQ1573830.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="AC1Y77_000221"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="AC1Y77_000221"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIQ1573831.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="AC1Y77_000222"
     CDS             37539..38414
                     /locus_tag="AC1Y77_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1573832.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="AC1Y77_000223"
     CDS             38680..40563
                     /locus_tag="AC1Y77_000223"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIQ1573833.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="AC1Y77_000224"
     CDS             complement(40638..40922)
                     /locus_tag="AC1Y77_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIQ1573834.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="AC1Y77_000225"
     CDS             complement(40912..41646)
                     /locus_tag="AC1Y77_000225"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIQ1573835.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="AC1Y77_000226"
     CDS             41775..42398
                     /locus_tag="AC1Y77_000226"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIQ1573836.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="AC1Y77_000227"
     CDS             42590..43711
                     /locus_tag="AC1Y77_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIQ1573837.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="AC1Y77_000228"
     CDS             43718..46846
                     /locus_tag="AC1Y77_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1573838.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="AC1Y77_000229"
     CDS             46865..50059
                     /locus_tag="AC1Y77_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HIQ1573839.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="AC1Y77_000230"
     CDS             complement(50205..50912)
                     /locus_tag="AC1Y77_000230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1573840.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="AC1Y77_000231"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="AC1Y77_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HIQ1573841.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="AC1Y77_000232"
     CDS             52407..53060
                     /locus_tag="AC1Y77_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIQ1573842.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="AC1Y77_000233"
     CDS             53286..54284
                     /locus_tag="AC1Y77_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HIQ1573843.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="AC1Y77_000234"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="AC1Y77_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIQ1573844.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18537 a         9724 c        11680 g        16406 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccgc cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 atttttt
//
LOCUS       DBNIBO010000005        55824 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.005, whole genome shotgun sequence.
ACCESSION   DBNIBO010000005 DBNIBO010000000
VERSION     DBNIBO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 55824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.005"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..172
                     /locus_tag="AC1Y77_000235"
     CDS             <1..172
                     /locus_tag="AC1Y77_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIQ1573845.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
     gene            complement(307..810)
                     /locus_tag="AC1Y77_000236"
     CDS             complement(307..810)
                     /locus_tag="AC1Y77_000236"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIQ1573846.1"
                     /translation="MVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQGFSH
                     NKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESNGKCA
                     YIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVISEKDK
                     NVERLSF"
     gene            complement(1004..1615)
                     /locus_tag="AC1Y77_000237"
     CDS             complement(1004..1615)
                     /locus_tag="AC1Y77_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1573847.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            complement(1641..3092)
                     /locus_tag="AC1Y77_000238"
     CDS             complement(1641..3092)
                     /locus_tag="AC1Y77_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIQ1573848.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            complement(3116..3847)
                     /locus_tag="AC1Y77_000239"
     CDS             complement(3116..3847)
                     /locus_tag="AC1Y77_000239"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1573849.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(3865..4260)
                     /locus_tag="AC1Y77_000240"
     CDS             complement(3865..4260)
                     /locus_tag="AC1Y77_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HIQ1573850.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(4362..5999)
                     /locus_tag="AC1Y77_000241"
     CDS             complement(4362..5999)
                     /locus_tag="AC1Y77_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HIQ1573851.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(6034..7368)
                     /gene="gmhB"
                     /locus_tag="AC1Y77_000242"
     CDS             complement(6034..7368)
                     /gene="gmhB"
                     /locus_tag="AC1Y77_000242"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIQ1573852.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(7410..8519)
                     /locus_tag="AC1Y77_000243"
     CDS             complement(7410..8519)
                     /locus_tag="AC1Y77_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HIQ1573853.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(8544..9677)
                     /locus_tag="AC1Y77_000244"
     CDS             complement(8544..9677)
                     /locus_tag="AC1Y77_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573854.1"
                     /translation="MNVRLVTVILAIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAY
                     IYSDKTIWVKSRESMSLYYVLLAYPLFCTLINQAWNLDSMLNTICGVTFLSYILRTSI
                     SLIQKFSGVLLYENIYRECAPENWYRGGFLRINPPCFTIIIIPIALYMIERQVVARRK
                     VKYYLLIASALAYSAVIHGSRSVLVYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVI
                     FINSTMFSTFVETFTNSTGEYAGSAESRFASVWFFIPKFLQSPLFGTGILTGEEFTFI
                     LPGGVRGHLSDIGFFYTITQYGVGILIFDILFIIKGFKTALLASKVGMTGYQYLAFGI
                     TLNVLLTGINIDWFYPIFTPAIPVCLAVIEYIYQECRSVANIE"
     gene            complement(9810..10916)
                     /locus_tag="AC1Y77_000245"
     CDS             complement(9810..10916)
                     /locus_tag="AC1Y77_000245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIQ1573855.1"
                     /translation="MKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRDVPPLDPKI
                     KSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYEGGLYVLCK
                     RHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAEFPELNAKS
                     YVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMDVRKKVHFT
                     IAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAIFPYKEIYG
                     SGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKYVNLSPAEK
                     SKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(10927..11700)
                     /locus_tag="AC1Y77_000246"
     CDS             complement(10927..11700)
                     /locus_tag="AC1Y77_000246"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HIQ1573856.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(11714..12169)
                     /locus_tag="AC1Y77_000247"
     CDS             complement(11714..12169)
                     /locus_tag="AC1Y77_000247"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIQ1573857.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(12591..13697)
                     /locus_tag="AC1Y77_000248"
     CDS             complement(12591..13697)
                     /locus_tag="AC1Y77_000248"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIQ1573858.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(13715..14476)
                     /locus_tag="AC1Y77_000249"
     CDS             complement(13715..14476)
                     /locus_tag="AC1Y77_000249"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIQ1573859.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(14552..15550)
                     /locus_tag="AC1Y77_000250"
     CDS             complement(14552..15550)
                     /locus_tag="AC1Y77_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIQ1573860.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(15569..16222)
                     /locus_tag="AC1Y77_000251"
     CDS             complement(15569..16222)
                     /locus_tag="AC1Y77_000251"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIQ1573861.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(16232..17242)
                     /locus_tag="AC1Y77_000252"
     CDS             complement(16232..17242)
                     /locus_tag="AC1Y77_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HIQ1573862.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(17261..18511)
                     /locus_tag="AC1Y77_000253"
     CDS             complement(17261..18511)
                     /locus_tag="AC1Y77_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIQ1573863.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(19857..19967)
                     /locus_tag="AC1Y77_000254"
     CDS             complement(19857..19967)
                     /locus_tag="AC1Y77_000254"
                     /inference="COORDINATES: protein motif:HMM:NF020874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HIQ1573864.1"
                     /translation="MNPNGYEWLRAKGSYPVPKYCNYSETLMEWYQLIIT"
     gene            complement(20373..21062)
                     /locus_tag="AC1Y77_000255"
     CDS             complement(20373..21062)
                     /locus_tag="AC1Y77_000255"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIQ1573865.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(21107..21871)
                     /locus_tag="AC1Y77_000256"
     CDS             complement(21107..21871)
                     /locus_tag="AC1Y77_000256"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIQ1573866.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(21899..22597)
                     /locus_tag="AC1Y77_000257"
     CDS             complement(21899..22597)
                     /locus_tag="AC1Y77_000257"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIQ1573867.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(22610..23389)
                     /locus_tag="AC1Y77_000258"
     CDS             complement(22610..23389)
                     /locus_tag="AC1Y77_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIQ1573868.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(23405..24349)
                     /locus_tag="AC1Y77_000259"
     CDS             complement(23405..24349)
                     /locus_tag="AC1Y77_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIQ1573869.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(24390..25169)
                     /locus_tag="AC1Y77_000260"
     CDS             complement(24390..25169)
                     /locus_tag="AC1Y77_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573870.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            complement(25554..27629)
                     /gene="glyS"
                     /locus_tag="AC1Y77_000261"
     CDS             complement(25554..27629)
                     /gene="glyS"
                     /locus_tag="AC1Y77_000261"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIQ1573871.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(27631..28548)
                     /gene="glyQ"
                     /locus_tag="AC1Y77_000262"
     CDS             complement(27631..28548)
                     /gene="glyQ"
                     /locus_tag="AC1Y77_000262"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIQ1573872.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(28934..29746)
                     /gene="recO"
                     /locus_tag="AC1Y77_000263"
     CDS             complement(28934..29746)
                     /gene="recO"
                     /locus_tag="AC1Y77_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIQ1573873.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(29889..30788)
                     /gene="era"
                     /locus_tag="AC1Y77_000264"
     CDS             complement(29889..30788)
                     /gene="era"
                     /locus_tag="AC1Y77_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIQ1573874.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(30803..31198)
                     /locus_tag="AC1Y77_000265"
     CDS             complement(30803..31198)
                     /locus_tag="AC1Y77_000265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIQ1573875.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(31182..31658)
                     /gene="ybeY"
                     /locus_tag="AC1Y77_000266"
     CDS             complement(31182..31658)
                     /gene="ybeY"
                     /locus_tag="AC1Y77_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIQ1573876.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(31675..33867)
                     /locus_tag="AC1Y77_000267"
     CDS             complement(31675..33867)
                     /locus_tag="AC1Y77_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIQ1573877.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(33891..34862)
                     /locus_tag="AC1Y77_000268"
     CDS             complement(33891..34862)
                     /locus_tag="AC1Y77_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIQ1573878.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            35568..36947
                     /gene="brnQ"
                     /locus_tag="AC1Y77_000269"
     CDS             35568..36947
                     /gene="brnQ"
                     /locus_tag="AC1Y77_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIQ1573879.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(37103..37549)
                     /locus_tag="AC1Y77_000270"
     CDS             complement(37103..37549)
                     /locus_tag="AC1Y77_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIQ1573880.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(37576..37752)
                     /gene="rpsU"
                     /locus_tag="AC1Y77_000271"
     CDS             complement(37576..37752)
                     /gene="rpsU"
                     /locus_tag="AC1Y77_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIQ1573881.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(37914..38342)
                     /locus_tag="AC1Y77_000272"
     CDS             complement(37914..38342)
                     /locus_tag="AC1Y77_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIQ1573882.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            38434..39294
                     /locus_tag="AC1Y77_000273"
     CDS             38434..39294
                     /locus_tag="AC1Y77_000273"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HIQ1573883.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(39388..39738)
                     /locus_tag="AC1Y77_000274"
     CDS             complement(39388..39738)
                     /locus_tag="AC1Y77_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1573884.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(39754..41481)
                     /locus_tag="AC1Y77_000275"
     CDS             complement(39754..41481)
                     /locus_tag="AC1Y77_000275"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIQ1573885.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(41695..42621)
                     /gene="trxB"
                     /locus_tag="AC1Y77_000276"
     CDS             complement(41695..42621)
                     /gene="trxB"
                     /locus_tag="AC1Y77_000276"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIQ1573886.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(42766..44199)
                     /locus_tag="AC1Y77_000277"
     CDS             complement(42766..44199)
                     /locus_tag="AC1Y77_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIQ1573887.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(44199..45602)
                     /locus_tag="AC1Y77_000278"
     CDS             complement(44199..45602)
                     /locus_tag="AC1Y77_000278"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIQ1573888.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            45832..46734
                     /locus_tag="AC1Y77_000279"
     CDS             45832..46734
                     /locus_tag="AC1Y77_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1573889.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(46775..48661)
                     /locus_tag="AC1Y77_000280"
     CDS             complement(46775..48661)
                     /locus_tag="AC1Y77_000280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1573890.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(48758..49609)
                     /gene="licT"
                     /locus_tag="AC1Y77_000281"
     CDS             complement(48758..49609)
                     /gene="licT"
                     /locus_tag="AC1Y77_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HIQ1573891.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(49944..51728)
                     /locus_tag="AC1Y77_000282"
     CDS             complement(49944..51728)
                     /locus_tag="AC1Y77_000282"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573892.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(51728..53479)
                     /locus_tag="AC1Y77_000283"
     CDS             complement(51728..53479)
                     /locus_tag="AC1Y77_000283"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573893.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(53621..53845)
                     /locus_tag="AC1Y77_000284"
     CDS             complement(53621..53845)
                     /locus_tag="AC1Y77_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HIQ1573894.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            54096..55682
                     /locus_tag="AC1Y77_000285"
     CDS             54096..55682
                     /locus_tag="AC1Y77_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIQ1573895.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT        16300 a        12025 c         9178 g        18321 t
ORIGIN      
        1 agttgagact gttttttctt ccttagaaaa attaggttgt caaaatttca actcacgttc
       61 tgttaaaggg ctagagagta gatttgaaag catattactg gcttacagtg ttctattaag
      121 tcgagcacaa cgacgttttg aaggaacttt gagatattct ttaggatatt aaaattaact
      181 agcaccacgg gtatatctat atctaaattt agttttatgt attcctcata gaaataaccg
      241 agcatttgct cggttattgt tcttcttatt ttccagtatc cttcttttct ccacagctca
      301 ttttaatcaa aagcttaatc tttccacatt tttgtctttc tcacttataa cgaaacactg
      361 ccgactgacc ggccattcaa tatctacgct ttcataactt atacatttgg cagcattaga
      421 attgaaatca ttatctacgc accactgaac tagcgtattg tcctccatag taagatagcc
      481 atgagcatat ccacgtggga tatacgcaca tttaccattg ctttcactca atgtgttgac
      541 atacacacat ccataagtgg aagatccttg tcgaatatca agtgcgatat tcataatttc
      601 accatgtagg cacatcacta tctttccttg agcatgctct ccactctgat aatgtagccc
      661 tcgcatagta tatttattat gactaaatcc ttgatttatt tgcttaatgt gaaactcaat
      721 accaatcgag aaaagagtct cattatccaa tgcaacagca gtatatcctc tgaaatccga
      781 aaacttaatt atatcaaaca cttttaccat aactaccacc atgttatata ttttaccaat
      841 tttttaattc gctcgacctc atttaaagca ataaaattcc ctacctcttg aagatataac
      901 aacaatgttt tagcaaaaat agatggcatg taaagtattc ttgatgagat tacacaatcc
      961 tgtctgcaga aaaatattaa ccatattaaa attcaaagca gttctatttt aagtctatat
     1021 acttatttaa tcggtagaat agccaatata gcaaagcacc aattaagccc cctagggtgt
     1081 tataaaacaa atccgatagt tgaaatgtac caagcctaaa aaacagttgt gtaaattcta
     1141 tacttaatga gaataaaaac acatacttaa tcccaacata gatggtgcgt ataatatcac
     1201 atttaatgtc acgccaccat aacaataaca tgcaaaaagg gataaacagc atcgtgttct
     1261 ccacgcattc tgttgtcaat ctgctctctc ctgtagcatt atctataata tataatgacc
     1321 actgtccaat aacgtttgaa agcgggttca tccacatatt tcgattaagc aaggttttaa
     1381 ataatatcaa agtaaaataa aacgcaagaa aaaagagtct gcgaaattta ctagatgctt
     1441 taaaatatct caaccagatt aatacagaat cttttaagtt tttaggatct cgagagttat
     1501 caactataaa tagataccag tacataaaca gatacgctgt taatacggca aataagaacg
     1561 gctgatataa tgctgttaat acatttaccg ttatagcaga aataatatct atcattctct
     1621 atctctctat gagcaaatgt ttatttgctc ataattctcc ttactaaaag tagcaatcgt
     1681 tttctaaata taattgcagc gacacaagca attgctataa ttaaatattt tatgaaatca
     1741 aatttattaa ttattgaaat taggatgcca ccagctatcg tgattgcaga aatcaaaagt
     1801 attcctctga catcatatat gttttttcca tccaatagtt ctttgcaaac ttttctataa
     1861 aaaatataat ggatgacgca gaaaaacata tatgagaata cagttgtgta tgctaccgcc
     1921 tgatacccat ataactttat aaaaacataa tttagtacaa tattcataat tgcacacaaa
     1981 accgatggaa taacgacagt gagtttcttt tcaaagtatt cctgtactct agcaaacagc
     2041 tgaaacatat agttgaaata aataccgata catataggta caattaaatt aacactgtcg
     2101 atatatttct gcccaccaaa tatcaacaca atttctttac taaatagcat taatgcaaaa
     2161 agcatagccg ctaccaaacc aagaacttca ttagctcttt ttgatagtaa atcatattct
     2221 cctgacttga ttttttgata ctgataaggt gcaaaagtgg aattgacaga atttgtaaca
     2281 aggttaatta gagcaccaat agaatatgcc acgctgtaaa tagcaacatc tgacgaacca
     2341 cataagtaat ttatcataat tttatcagaa ccattcagaa taaattcaga taaatagtgt
     2401 ggcatcaacg atacacaaaa tactactgcg aaattccaca cttcattatc cttaaaatcc
     2461 ttacatcttc taaataccga tatatacaca gccacgccaa caacaccagt cactaggaca
     2521 ttcattaagg ctctcgcctc agcggtagga gatataaaca gaaccgctaa acagccaact
     2581 acagatgagc caacagtaac tgcaagggta gctactaata aagtcttata atcatatata
     2641 tatcgtttgt gtaaagacca acattgtatt tctgcctgaa atataaaata tgagaacata
     2701 cagacaacta gaactggtgt caatcctaaa atttctgcca atatatcctt aataaaaata
     2761 gcaataacaa accaaaaaat agtcagtact aaagtcaatc cggatactgt cgaaagaata
     2821 tgttcttgat tatcatattt tacataaaga ttcatcatgg ctttgaacaa actaagcgat
     2881 gtaatcatga gcaataagga ttcccacgat agataagtgc tgtataaacc gtattgttct
     2941 gtagacaaaa ctctagtaaa aattggggtt gtaatcattg aaacccctct ctgtaaaaaa
     3001 ccacatacca taaaccataa tgcagcctta acaggaagtg gagtattctt atatctgctt
     3061 aatgttttta caattagatt attactggtc actgctattt tctcctaaac actatttatt
     3121 cttgattcaa aaacacattg agctcttctg gagtaccaag cacatgaacc tttttcgcat
     3181 ctactatgct aatagtcacc tttaaccctt tttttatcat aaagttatat aatggtgcta
     3241 tatatttttc attcttctcc atattagagg agtctgcata atactcttca tataaagctt
     3301 tatacagctt tgccgatgag aaataatacg caccaagagt acaattatca gaaatgcgaa
     3361 ctttttcgcg gacttctatt acttcatttc gatcgttaag ctttgcaaaa ctccaatgat
     3421 cgccatcagc atgaaagcat ggaatatgtc catctccaca aatatcttcg tatttcatct
     3481 catttgattc cacgtatgta tcaatattgt aaaccataat ctcattgtct tgattgcaat
     3541 aaggaatagc caacatacat gtagttgctt gaccatctgt catatagtct atttcaatca
     3601 cttctacgtt ttcaatacca aagtaagcac aatgctcctt tataaaagct gaagcgttgt
     3661 cttcctgacg aacaacaaaa atatacttat caacattttt attgtatcca accaaactat
     3721 ccatggccca atcaaataaa gttcttcctt tggcattaat catatattta ggaaccgcat
     3781 aaccaatttt tcgaaaacga cttcccaaac ctgccatcgt aatgataact gtcatctttt
     3841 tttccataga actaacctcc ttatttagaa tctttttttt gaactgttgc agatttcatt
     3901 taattcatct acagagcatc tcaaaaactc atcaggtcta accgttcgat catctacata
     3961 gaagccttta tgccccggcc aaggcttacc atacaggatt tcatcatacg gaatgtccca
     4021 tttatccaac cacgccaaga gcacttttgc agtattagca tttatcattc ccaaatttcc
     4081 tttataggaa ttaacattgc gtgaggtgaa caatgtaatt ttagcaccat tctccttata
     4141 gaatctcagt ttttcaacca tattctcata cggcaccaaa tcctcatatc gctcatcctt
     4201 cttctttatc ggacacaaag tgccatcgat atcaaacaca aaagaatatt cactatacat
     4261 ttttctcttc ccttctattg tattaattaa tataatcgtg ccttaatgcc tacattgcta
     4321 aaacagtatt gtaaccgtta ttcattaagg cacaatttat attagagtat aactttatta
     4381 cttactcttg caagaagttc catacctgta gcaagcatca ctatttggtg atcaaaactt
     4441 tcaccgtgta atggaatcat actaagaaat agcaacgctt ctatcaatac aatcttatca
     4501 aaatcgtcct taatctcgtc cgaaaagacc gacttaaaca attcaaataa atcgtaatct
     4561 cttacacgcg tattaattgt ataatctatc actgcacact cggtatcata ttctacagta
     4621 aatagatcct tgatgatata atcatattta ccatctaaac tatgaaatag ttttgctaat
     4681 tcatatctgc tgtccccata aatatcatat tttccaaatt ttcccctagg atcaataacc
     4741 ttaacaaagg aacaattgga atctaccatc atattagcaa aacacaaatc tccatgtata
     4801 atacaaaatt catttacatc atagagcata cttgggataa gattctttag aagttcaatt
     4861 acaccggtta aagaaataaa cttacgacca ttaatttgaa tttgtttctc aaaaaaagct
     4921 gaaaatcttt tatcttcaga caatttttga aatctctgca tggtttttcg caaatacata
     4981 tcttccaatg actctctaat cttttcatct tttaaggtgt atcgcttaaa atcgctacag
     5041 ataaacttta tacggctaaa cagatcatgc cactggttat attttaaatc accatataag
     5101 tataactcat gaaccgtatg atatgcataa tattccatat caacataggg agctacgtaa
     5161 gatgtagaat aatcaaaaat tctaggtctt acatattcaa tatcagcagg aagttttaaa
     5221 taccacttta tttctccaat aaatttatcc ttgtcatcgc tagttttctt caatatccct
     5281 cgatctttat ctatagagat atgattaaac tctcgtgctt taacttccaa ctttgtattg
     5341 taatatttat cgatgtgtcc aatatcaaac cacttttctg ttcttacggc ctttagcgga
     5401 caacgttcgc tgtatagctg taaagcccga taaaatgagt ttattccccc ctgtttccga
     5461 ctctcaactg cctctcttaa gcaactcttc agaagttttg acgaagaaaa cataaatacg
     5521 ccaataaata atttttcacg tttatcggat acgtaagctt cctctttatc gtggatatca
     5581 gtaattattc cttcattaat tttaaagtat gtccaagtac tcgacacaaa atcttcagaa
     5641 taatagaatg tatccatttc tatatcagac atactctcca taacaattgt atctgcgaag
     5701 ttaattagaa caggctcatc ttcatctgat aacccaaaat acacggtttg ccctagatcc
     5761 cctaattcag gaaggtcaca tatatgaatt ctggaatcat gatagttttt cagtcttcta
     5821 tgaaccttat ccgctttttc ataacagata atatccaatg aatcgcagat tgcattatac
     5881 tgattataca aataatcaaa gacaattttt ttattaattg gatagataat tgctggtaac
     5941 attcctatac tctgcaattc tttttcaaca acttttgctg ctggaattaa taccttcatc
     6001 taattactcc ttttacaagt ttcactaaac caattattgc atagtgcata aatgattttt
     6061 aacaaattct aacaatgaac agacactata gtcttggcca taattagcag gcaaggtgat
     6121 gtcttctgta atcagtactg tcttacaacc agcatttttt ccagcaatta catcactttc
     6181 actatctcca accataaatg attgacttag gtttatgtta aaatcctttg atgcttctaa
     6241 taacagtcca ggttttggct tgcgacaatt gcaatcaatt tttaattcta taacctctcc
     6301 tgcatatcct ttatggggat ggtgcggaca gtaataaata ccgtttaaaa atccaccagc
     6361 atgccctagc attgtttcaa gcttattgtg aatactatta agctcatcaa cagtaacttc
     6421 acctcttgca attacaggct gatttgttat aacaatacac agaaaaccta gagaattaat
     6481 aattttaatt gcctccgata ctccatcaat taactcaaat tcttctgcat ttctcaaaaa
     6541 accaacgtat ttattaatcg ttccatctct atctaaaaag attgcttttt gtggattgga
     6601 tagattttta ccaacaataa ttcccttttg aaagtcctta attacagctc tatatcggtc
     6661 cggtgttccc atatctttaa catactctgg agaatcgtag caatacatac gccctgtgtt
     6721 agccaatggt ttcagaatca ttctatctaa atcaattttg tatggtagcc ccccaccttc
     6781 aactattgat ttatttacaa tttccattaa tggattttct ctcaacacct tcggatttat
     6841 aacatgaaga cctgcattta ctcggttttt ataatattca ggtctttgat cttccttcgc
     6901 taaccattga agcactttct tattgtcatc ggatataatt aatccactat catacggatg
     6961 tgagttagga tgcgtaaaca aagtaacatc tgcgttattc tcaacatgaa atctaatcat
     7021 ctgcctgaaa tcaacatcaa atactgcatc agcattcaaa agtaaaaacg gttcatcacc
     7081 cagttcatcc aacaaaaaat acaatgcacc tgcattgcca agtggagttt tttcaataaa
     7141 atatttgatt ttaattccta attttgaacc atctccgaaa tgctcctcta ttttttcatg
     7201 caaataggaa acagtcaaaa taatatcatt aaagccttga tctctaagcg aacacaattc
     7261 tcgttctaaa actggaatgc catctatagg aatcattggc ttaggaatat ccggaaacaa
     7321 ttccgaaatt cggcttcctc gtccgcccgc cataattact gttttcataa tatgcccttc
     7381 cctaaagtac tttttactat aaaagttaat tactcttccc aaacaaagtc catatatttt
     7441 tcatattctg ctttgtattc gtattttcca gtgtacaaat tcaaccaatt tataaaatca
     7501 aaattatagc agtgaagtgc atcttggcta tcatcaatat aatacgtacc ataaatagta
     7561 ttatttgaat aacgacctac atatttatgc cagtacaaat caatttgatt tttgttaata
     7621 gtaataacat ggttttttac aaactcccaa tatgccctaa tagagcactc ctcattgccc
     7681 cccattcgtg aaatatagct tcgaagaagc tgaacttctg gagctaaatt attttcagat
     7741 atctcccttc ttgagactcc tctagacaca gctccctttt ctctcacatc actaggaatc
     7801 gagaagaggt ttatcatatc atcaacatca ccaaaataca ggaagtctga caaacaatac
     7861 gggaaaaaca tgtctccata cggcattgat agtaacagta atcgctgatt ttgtattaat
     7921 ttccaatcat cttcgttatt aacaggatac tgttcaagta aactgcagaa aaacgccatt
     7981 gcatttggat ggtatattct ttgatctgtt ctagttttac aaacatactt tgcaccaagc
     8041 tgctttgctc tgactattcc aggcaaagtg tttccaactt gataattaat atttaagtgt
     8101 ccaggctttt caggtttctc actacaaata actataactc caagatcctc tatccttttc
     8161 aatgccttaa tatcttcgtc cttccatgtc gaaagaataa ttaaagcatt tggataacat
     8221 ttcttatagt aggccagagt attataagag aaatcatcct ttaaacatag tggcccctgc
     8281 attacaattg ctacatcttt gcaacaatta actaaaccca tcactggaac atcagacatg
     8341 tcctttgagc gcaattgaaa cgctgcataa acttcagaat tttcttctac tgttgcacaa
     8401 tcagcagtca atttccaaga tacgagttta cttatattat gaccaaacat gttcctaaac
     8461 acatatggta aattttcgta cttcttcaat atgttatata aaatctcttt cttattcatg
     8521 agatgcactc ctattaatat ttcctattct atgttagcca cacttctaca ttcttgatag
     8581 atgtattcta taaccgctaa gcatacagga atagctggag taaatatcgg ataaaaccag
     8641 tctatattaa tccctgttag caagacattt aatgtaattc caaatgctaa atattgatag
     8701 ccagtcatac ccacttttga tgcaagtaat gctgttttga acccttttat aataaacaga
     8761 atatcaaata taagtatacc tacaccatat tgagtaattg tatagaaaaa tccaatatca
     8821 gataggtgtc ctctaactcc tcctggtaat ataaacgtaa attcctcacc agtaagaata
     8881 ccagtaccga acaaaggcga ttgcaaaaat ttaggaataa aaaaccaaac tgaagcaaat
     8941 cgagattcag cagatcctgc atattcacct gtactgtttg tgaaagtttc aacaaatgtt
     9001 gagaacatgg tcgaattaat aaatatgact acaagcaacc cagctaaaac ccacattgca
     9061 atttttcttt tcgaagaacc tttcttaaaa aggatatagc aaccaagtac aataatttga
     9121 tagacaagga cagatctaga accatgaata acagcagaat atgccagtgc gctagcaatc
     9181 agtaaataat attttacttt tcttctagct acaacttgtc gctcaatcat atacaaagca
     9241 atcggtatta ttataattgt aaaacatggt ggattaattc ttaaaaagcc tccacgatac
     9301 caattttccg gggcacactc acgatatatg ttttcataga gaagtactcc tgaaaatttc
     9361 tgaataagtg aaatagatgt tctaagaatg tatgaaagaa atgttacacc acatatcgta
     9421 tttagcattg aatccaaatt ccacgcttgg ttaattaaag tacagaacaa tggatacgcc
     9481 aataatacat agtataaact catactttcc ctagatttaa cccatattgt cttatctgag
     9541 tagatgtaag ctgctattat aacaacacaa atcagtaaag tgcagaaatt taaatatcta
     9601 ttttttctaa atgctggtaa tgaggctatt aacaaacata ctattgctag aatgactgtc
     9661 acaagtctaa cgttcatcgt ttctcccaac cagccgccca aaggcttcaa tatgcttgat
     9721 gagataatca ttagaaagac cattagaaac tgtatgattt tccatttact aatctgcatg
     9781 ttaattatta ttcctttcta attgttttac tactcattca aaatttgttt gtatgcctcg
     9841 tatgtgagtt ttgcagatgc cgcccaggta tacttgcggt ttacaagctc atatatatca
     9901 tcctctattt tgcttttctc agctgggcta aggttcacat actttacaat tgtgtctgct
     9961 aggctcttag aattcaaatt ttcgaaaagt aaccctatct ttccttcctc ggtttcttcc
    10021 ataaaagcag gtacattact aacaatagtt ggtaagcaat aagtgtatgc cataagaagc
    10081 gctccacttc catagatttc tttatatgga aatattgcgg catctgcatt tctgaacata
    10141 ctgtccattt cttcatcaga aattctctct ggcttatata gaacacaatc tgaaaccttt
    10201 aaatcttcaa tcattttttt atagtcagta ctatcctgtt taggatgttg ttgacctgct
    10261 atggtaaaat gaactttctt tcttacatcc attggaagtt ctgtgattgc attaagcagt
    10321 acatcaattc ccttatatgg acgaatttgt ccaaacaaga taaattcaaa cttatcttta
    10381 tcatacgctc tagatatatg tttaacatct ccatagatag catgtggaca aacatatgac
    10441 tttgcattta attctgggaa ttcagcatat aaatgctgtt ctgttactcg attgtgaaca
    10501 atgaaaccat cacaatttga atatatatta aagtgctctt ggcgatcctt ttcacgaacc
    10561 tcatgtggta acacattatg aactgtcata attaattttt tatagtgtct cttacataaa
    10621 acgtacaatc cgccttcata tttgagactt ttcatcaatt gcaaatgaac aatatcataa
    10681 tcaccaaatc ttatttcctt ccaaattaag aacaagcttt tcagaaaaac aagtgccgaa
    10741 aacactttat ttgtactttt atgatacaaa atatgtttgc tcttaatttt aggatctaaa
    10801 ggaggaacat cacgcttaca cagcaatgtt aaatcaacat attgattaag ttctttagaa
    10861 agatcaaacg aataccaatt ctctggcatt tggctttcac acatcaatac cttcataata
    10921 ctcaccttat aaccaatcaa cagaaaattc tgctttcagt tttcctttaa ttttacgttt
    10981 tgctataaac atttccagta tctctcgcat tttcgtctga tattttgtcg ttttaccata
    11041 aagcgagtga ttgtcccctg ttcctaatac atatactgct gctttgaatg gaagaggttt
    11101 taatggacgc ccttgctctt cacatgctgg aacaacgccc ccatgtcgtt ttctaatgat
    11161 ccatggatta tcatcacaat ttgctgtcat aacttccgga tatgcctcag gcaggtcttc
    11221 cactgcatat tttactatac acgcacttcc tgatgagaac tttggtagaa ccttcatata
    11281 atcgtctcca acaaagtaca cgtatcccgt tttgacataa aatccatttt cctggggatg
    11341 ggaattcaca tattccgcca aatgagaact tacaagatca tctgcgtcaa ccatcatagc
    11401 gaaaccgccg cccaactctc gcaaacgcat tgcgagagca tgtgttttat aacccttatc
    11461 aatcattttt tcgtcttgat ttgtgggaat aggggcatcc ttttcgctaa cttgaataaa
    11521 ttcaactctg tcatcatatt catgcgtgag tttaggaatt tcatggcaag caacaatgat
    11581 tttgaaatca ggatcagtct gattataaca cgaccataat gttcggttaa ataaggtact
    11641 aaccatctcc caattgtttg ctgccttcgc actcattaaa ggaataacaa aatacaacat
    11701 acaatctctc cttttagtaa cgattcaaac tacaataatc atttacatca atatcttttt
    11761 tcaatactct agccggaacg cctcctaccg aacaattagg ttcaacatca ttaatgacca
    11821 cagcattagc ccctactatt acattgtccc ctattgtgat attacctaaa atttttgcgc
    11881 cagtagcgat atatacattg tttccaattg taggcatacc aggcttattg gatctaccac
    11941 cagtcagtac attcgttccg attataacgt gattacctat ttttgtatct tttccaatta
    12001 cacaacctat gccaccgtat aagaacttac aattatctcc aatttctgtt tgatatgaaa
    12061 ttgagctatt atgaagtaca taaattagtg cctttacaat cttggcaaaa aactccattt
    12121 tgtgtaagta aaaccatcgt tctactttgt ataatttata tgcattcatg ttttatctcc
    12181 ccatcttttt agcaggtacc ccccacactt cgtagggtgg aatagacctc aataccactg
    12241 tatttgcagc aactacagca ccttccccaa cgcataattc tgtgttagat ttacaaagaa
    12301 ttttagcacc cacacataaa acagcgttct tttttactcg aatgatgata cgttcattac
    12361 tatctgctca accacacgtc acattctgat agactttaac accatcttca aggattgtgt
    12421 cagaatgaat cacagtgcct acagaattat gaacaaattc aacagagttc cctatctgtg
    12481 cacctttacc aatacaacaa ccaagaattt tggctatttt tctcgtgcaa ttatcaacta
    12541 tgtttaaagc atatcgattc ctcaatgcca ttaatttcgt tacttccata tcatttatca
    12601 ccttccaaca tgctaagcat aatttctccc gactttgacc atctaaattc tgtagttaca
    12661 cgctgatatg ctgcatctat gatatcgttg gtaaatcgtc cgtcttttaa gaatgtcaaa
    12721 atttcatcgt cagatttagc caacattcca tatctattat ccaaaataat attctgtggg
    12781 ccaggtgcat taacagcaat aatcccgagg cgatagtaca ttgcctctag caaaaccata
    12841 ccccaaattt cagtatagtt tagatttaca aatacatccg ataagcaata taactcccaa
    12901 atatctttgt taggaatacg ctcaattatt tttactctat tctgcagtcc aagtaaattt
    12961 atctgctgct tcgtttgctc gagtaaagca ccagttccta ccatcagcaa ataattatgc
    13021 tcatcaagtt tattaaataa actaatcata tctaacggtc ttttatcctt ttcaaaccgt
    13081 cctacatata aaataacacg aacattgcga ggaatattcc atttttctct gagagtagta
    13141 aaatctattg cttcgtaatt cttatgtaac tgttcaaaat ccacacaaac aggagcaagt
    13201 ttcactttat ttactcctaa ttgtactaat ttttcagaaa ctcgatcatt tttggcaaca
    13261 cacccatttt tcttataaat tttataattt cgatgcgcca atatttcaat aattgtcttt
    13321 atatatttat tgccactttg acttcgcgtt atcccattat aaggtatata ttttattcca
    13381 ttttttacag tccatgagta tacatgaggt actgatagtt gaaggtccgc aaattgtatt
    13441 agaacatccg cattcttatc aaaattcttt gttgataaga atccatttga tcctacataa
    13501 cgacacttga tatagtgaat catcgccgta ttgcttaatt gctctgttcc aacttcctca
    13561 tcacttttta cacttttata tacaataact tcgtggccta attcagctaa ccctcgagct
    13621 aatcctattt cctgagaatt ataggacccc ttcttaccaa agttattcgt cattgtaact
    13681 actataagta ttttcatcta tttttattgc ccccctactt agattcaata gtatagtaga
    13741 tatactttat acctcccaag atataccgcc tccatagtct acgaggttcc tttaccagtc
    13801 taaataacca ctctagacta gctttttcca tccattcagg agctttttcc aaagtgcccg
    13861 caaaaaagtt aaagccggcg cctacaccga acattactgt gttatggatc ttcttctggt
    13921 gttcccacat ccacatttct tgcttaggcg caccaatacc aacccacaga aaatctggat
    13981 ttaagttgtt gatttcctcg caaatcttgc tatcctcttc ttcatttaac ggacgaaatg
    14041 gtggggaata cattccaaca attttgattc ccgggtaatc tctttctagg tttgcacgaa
    14101 gcttatttaa cacttcgtta tcttttccac catagaaata atgtgttttt ccctgtctaa
    14161 cgccttcttc aaaaaccatt cccataatat ctggtgcagc acatcgttca caggtaaatc
    14221 ctttgcgccg tccaatttta acaataggca taccatctat tgcagccatt gtagcctggc
    14281 tatacatttc agcaattttg ggattttcct ttgccgaaat aatggccggc actcccacga
    14341 gatcaacagt cgcacttacc ggagatgttg gcatggaaaa aacttccctt acaaactcgt
    14401 tctttgtagt agcacaaaca cctactattc cacacacagt gaaatcacgt tttcttttat
    14461 acatattctt ttccatctca gtacatcctt actcatatgt tcttcctccg catcctctaa
    14521 ttacacggag gaagaacagc ttatatactt attaaccctt aatctgcttt tcaatccaat
    14581 tgtaggattc acgaagaccc tcttccaaag aaacagtagg ctcccaatcc aaaatctctt
    14641 tcgccttaga ataatctgcg caacgagcct tatcaccttc aggcttagaa gtatcaaaga
    14701 aaggcttgat gtctttgcca gaaatttcaa tgatcttata tgcaatttcc ttgatggaag
    14761 tacagtgaga agggccaatc tgaatatgac catgacccca acccttatcc agggcgagca
    14821 ccaatgcatt tacgatatca ttgacatgaa tgaaagcacg tccctgctct ccgctacccc
    14881 acacattaaa aggctcttca ggattattga ttgctttacg aatcagagca ggaataacct
    14941 gactacgttc gccaaaatca gtaggtgtgc cataaacatt atggagcatc aggatacagc
    15001 acggaattcc ggtttctttt tccaaatacc ccatctccaa ctggcccatc agtttgctcc
    15061 agccataagc agactcagga agagcaggga acaactcctc ttcacggaga ggctcaggat
    15121 tcaaagtatt ctgtctggtc agagggaagg agcatacggt accaacatag ataacaccct
    15181 tgattttctc cttgccagcc ttacggcaac aatcaaaaac attagaattg atcagattat
    15241 tgattcggaa caattcgccc tggttcttaa acacatagtc aatgccagcc acaacatcag
    15301 ccagatgaac tacatactca gtcgtaccaa taaccttttt agcctgctca taatcagtca
    15361 gatcagcatt gaagaaatga gtatccaggt cgatgacagg atgcccatct tcgtcgttca
    15421 ggtactccaa ctttcctctc cacagattat cagctacata aacatcccag cagccttcct
    15481 tcaccagtct cttaacaaga ttggagccaa tcataccgca accaccggta accataattg
    15541 tctttttcat ttaacattcc tccaaatttt attcgtttcc aaagaattta tcttccagca
    15601 tcaaacacca acaatgatat atcggcaagt gatactcttg aatcatgtat gtttcaactt
    15661 caggagcctt aacacaaaca tctgctaccg tggcaagttc cccacccttt gcgcccgtca
    15721 agccaataac ttttataccg agagccctag caacaacagt tgcgctcata atattcttgc
    15781 tatttccgga cgtcgaaata ccaaggaata cgtcaccaga tctaccaaag ccaaacagct
    15841 gctgtgcgaa gacacccaaa ccatcgacat cattaatata agcagtagac aatgcttcgt
    15901 gtgcgaccaa aggaattgcc gttaaaccac actcgagatt tttggctaat tccggacctc
    15961 ttacagggtc aattgcaact aacttatcgg caaattcctt acttacagga cgtggcgtct
    16021 taaaccgctt catcaactcg ccagcaatat gttccgagtc agcggctgat cctccgtttc
    16081 ctgccacaag aagtttacca ccattcaaat agcattcctc cataattagg tatccatcga
    16141 taatgtcctg tttaatcgtc tcaagtgtag gatatcgttt aatcaataag tcaatatgtt
    16201 tattaagttt ttccgctaac attgttactc cttatccatt ctgtttttca atataagttt
    16261 ccggcgtgta gtgaataact ctagttccac cattttcaaa ttcaaacgga atatacatca
    16321 aatcacgcat agcccaacga actgcatcct gtttctcagg ttgtacatag aaaaccaaga
    16381 aacctccacc acctgcaccg agcaactttc cgcctaaagc acctgcagcc ataccctttt
    16441 catatagttc atcaatgctt cctgtactaa ttgctccacc agtacctttt ttcaatctcc
    16501 aagtaacatc aagctgtcta ccgaaatcat ctaaatttct gttcttatca gtaagaattg
    16561 cctcagcttc atctacaagc tcatacatct ttttcagtct tactctctta tcctctgtgc
    16621 cactgacgtt gttagccttt tgaacatcag aagaaaatct agtgaatccg gtaaagaaca
    16681 tcatcagatt gttgttaagc tgctttttac gttcaggcga aataatgaca ggcagcacct
    16741 cataaccatc cacattgaaa ttaattcgat tgaacccacc ataagatgcg gcaatctgat
    16801 cctgccatcc accggcttca ttacacagaa ctctttcaag ataaatggct tcatcagcaa
    16861 gtttcttctt gtcagcatac ttaccctcca gtgcataaaa agcattcaac ataccaactg
    16921 caaaagaact ggatgtaccc agaccagagc gagcaggtaa atcagcttcg tatgtaagcc
    16981 gaatttcatg catatcgagc attttcatcg cattacgaat cgcaggatgc tcaatatctt
    17041 caatgctagt tactctctca gtacgagaat aagataactc agcgctgtaa tcaaaaaatc
    17101 taggcagatg tctcacattc acataacagt acttatcaaa agtagtagaa agaactgcac
    17161 cgccattctc tttgaaataa tcctccatat ccgttccacc gccaaaaaaa gacatacgaa
    17221 acggtgtttt tgtaataatc ataaatactc tctcctaatc ttagtctctc tggaagatat
    17281 cacgggtata tactttttcc ttaatgtcat caaggcaagc atcgtagcga tttgctataa
    17341 cagaatcaca cattgcctta aactgttcca aatcattcac aaccttactg ccaaagaaag
    17401 tctctccatc tttcaaggtc ggctcataaa tcacaacagt tgcaccttta gcttttacac
    17461 gcttcataac gccctggata gaactctggc ggaagttatc agaattggat ttcatggtca
    17521 aacggtaaac accaatcaca acaggctttt ctttgccagc atcatactga tttgcttctg
    17581 cgtaattgta ataaccagcc atctgaagta cacgatccgc aataaagtcc ttacgagtac
    17641 ggttagattc aacgattgcc tcgatcagat tttcaggaac atctgcgtag ttggccagaa
    17701 gctgcttggt atccttcggc aggcaatagc caccgtaacc aaaggaaggg ttgttgtaat
    17761 gagtaccaat acgaggatca aggcaaactc cattaatgat ctgctgagtg ttcaagcctt
    17821 tcatctctgc ataagtgtcc agctcattga agtagctgac acgaagagcc agatatgtat
    17881 tggcaaacag cttaacagct tctgcttcag taaagcccat gaacaatgtg tccacattct
    17941 ctttgattgc gccttcctgt agcagtgtag caaaagtgtg tgcatcctca accaaacgct
    18001 catcatccat atcggtaccc acaataatac gcgaaggata cagattgtca tacagtgcct
    18061 tactttcacg caggaactca gggctgaaaa taatattctt gctaccagtc tttacacgaa
    18121 cagaagcggt gtatccaaca ggaatggtgg acttaataac cataatggca tcaggattta
    18181 cttccatgac cagcctaata acagcctcaa cagcggatgt gtcaaaatgt tgagtgcggc
    18241 tattgtagtt agtaggagcc gcaataacaa caaaatccgc agactggtat gctgcctttg
    18301 catccaatgt tgcggtcaga ttcagttctt tctcagccaa gtacttttca atataatcat
    18361 cctggattgg agatttacgg ttattaatca gttcaacctt ctctggaatg atatccaccg
    18421 ctgtaacctt atggtgctgt gccaacagag tggcaatact caaaccaaca taaccggtgc
    18481 cggcgactgc aatgttatac tgtttcttca taatatttcc tctttccacc actgctgtgg
    18541 tcactacttt cttaacaaaa tgctgtttgt gaacagtaac ctgctattta tataaaacag
    18601 cccgaaagct gtgttcattc atacttaaaa cagccagctc ctacgccggc aaattcaatt
    18661 tttcataaat cccctacccg tttagggtta tattgcaagt ttgagtcatt tttattgacc
    18721 tcgtttgggg ttataataca ttttcgcatt gaaatgtacc tacattttcc actcaaccag
    18781 tcaccaaaaa gtttataaat tcacttatta ccctctattc ttactccaaa ggtatgttta
    18841 acatcgcttt attgacctat tttcacccca aatggaagtt tgtttggtac attattggtc
    18901 tattcccacc ccaaactgtc tattttgagc gagtttttgt tttttatcat tgcacatttt
    18961 caaggctcaa attctaagcg gatttactgg cagggagaca ttggtaaata cttgaacaat
    19021 ttcacggctt tcatcatcac atcctacgat aataattcta attggataaa gattatcatt
    19081 aataatctac tttcacgtaa aaattctgaa ctaaataaaa tctattgata atattttttt
    19141 cttccacttt ttgtatatcc tacaggtata gtggatttaa ttatccccaa tgcctctgaa
    19201 gtaacagaca tcactaattc aataacacat tcactaccat agtttgtagg aacaaaaatg
    19261 acaacaaaat ctgcttcctt atgtgatgtt tttccatctg tccaaatttt attaccaatg
    19321 aaatctagtt taaaatccct cttagaaact agttcactct atatttttat tcagtttcat
    19381 tagttctgtt tttttgtctt cagtcaccat atacatatat atcttaatta tctatccatt
    19441 tcatcctacg cattcttgcg taaatacaat tcactctgtt gagctgatat aaatcattgg
    19501 tggtacaaag ataaaatgat aactgtttct atacctccaa aaattccagt aaatatatct
    19561 atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat ttaagatagt ggtacatact
    19621 ttataacaac gtacttttcc aaatattatt ttccgttatg aacatccaat atattacaaa
    19681 gtttaaaagt ttagatgaac tataatcttc atttctgcac agtggataat aaattcttat
    19741 agataaaaaa tagcctcttc taaagtcaca aataccttac tcccacattt taagtaaaca
    19801 ctctgcagca ttctctttgt tataaaatga tagatattcc tcaattttat ctcttattat
    19861 gttattatca gttgatacca ttccattaat gtttcagaat aattacaata ttttgggacc
    19921 ggataactcc cttttgctcg taaccactca tatccgttag gattcactaa aagtttaccg
    19981 tttatttttc gattcttttt ttgaaccgtc ccatgaacat accgattcaa caagctaaat
    20041 tataaaaatt ggtgtgctgt cattattttt tggaaaattt attaatggct atcaatatac
    20101 ttttgtatca tactaaatag ctcgtcagtt cctatatcgg atataccaat attaaagcat
    20161 ttagatcatt atatttaaga gtggtatcat cccaaaattt cgaatatttt cttttatata
    20221 agaataatga tactgaattg ccccattcac ctttcttttt ttagattttc tacaagtgat
    20281 tcaaaccctc cataattaca gttattactt ttgaaacgat aatatgaaca tgtgtaatct
    20341 tttttattaa caatccatca ttctatccct aactaatatg ctccattggg cttgacaata
    20401 acataaaacg tttttaaaat caacctcaag tcgtacatta aacttcgatg actaatatac
    20461 tctaaatcca actcaaccat tttatggaaa ctaacattat ttctatcact aacttgccac
    20521 aaacctgaac aaccaggttt tacatacaag cgttgaagat catatctcgt gtactcagct
    20581 acttccctct ccaaaggtgg ccgtggacca attagactca tatcgccttt taaaacattc
    20641 catagctgtg gcaattcatc aatgcttttg gcacggataa ttttacctat tttagtgaca
    20701 cggggatctt ctttcatctt gaacattgca ccttctattt cattttgatc aagtaattct
    20761 tccaattttt cttcagcatc taaacacatc gaacggaatt tatacatttt gaatgttttt
    20821 ccatttttac caacacgtgt atggcaaaaa aatatcggac ttcctggctc ttcttttctg
    20881 attcgataag ctacatagaa catcaaagga gaagctaaaa tcaaaccaat aatgcttcct
    20941 acaatatcaa tcattctttt aaaaaataaa tagatatatc gtttttcaac tttttcttta
    21001 tccaaccaaa cttgattagg atgtacttca ctaatacgag ataataaatc ttcactttcc
    21061 atactttccc tctctcctca tttccttttt atctacgaat aaagaactaa aataaaccaa
    21121 acttcttctt ttttacctct tcatagactg gataactgat ttcatccccg ttgatcagat
    21181 cctttgcgac ttgtttcatc tgtactactt tttccttacc aaagtcctta gcaatctgct
    21241 catagcactc ttttaagtaa aaggtacgct tagagacccc atgggcatca gaagcagcca
    21301 tctgcacaag attatgtttg accatcattt tggctgtttt ctggatgtct tttccaaata
    21361 caccgacata acttggtgcg gtgagttggg ccaagcatcc catatctagg aatggcagca
    21421 tatgattcgg gtctttacga aaatgcgcgt tgcgttctgg atggacgatc actggtgtct
    21481 ttcctagttg taacaactca aaaaagagct gttctgcata taacggcact tccagtgtcg
    21541 gaaattcgat tagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtcga
    21601 tgtcttcgat cagtgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca
    21661 attgtctttt ctctaattct gcttgtaacg aagcgactag agaaatgact tgtgattttt
    21721 cattgctgta ttttccatta ttatgatggg gtgtgcataa aatatgtgtg atcccttgtt
    21781 gaatcgcttt ttcagccatg gcgatactgg catctatgtt ttctgctccg tcatcgattc
    21841 ctggcaggat atggcaatgt agatcgatca tttctcttgt ctcctctctt tttatttctt
    21901 aattcccgta gtaatagcca gcatctttgc tgtgttctgc cccattgtat acaacaccaa
    21961 ggactcgcgc ttgtaccata tccagcaatt cttttgcttt agtcaatgat tctttccgtg
    22021 acatattttc acgtacgacc aataaggtgc catccgtttt ggaagacatg atctgtgcat
    22081 ctgttacggc aactactggc ggcatgtcga agatcactac atcaaatagt tgtcttgctt
    22141 ctgctaaaat ctgatccatt cttggcgaac ttagtagttc tgatggattt ggtggtttag
    22201 gtccacttgg cagaatcgac aaattctcga ctggtgttcg ttggatctca tctgctacac
    22261 ttcctgatga actaagaacg gtacttaatc cgcttgcgtt gttcaacttg aacgttttat
    22321 agatgacggg cttacgtaag tctgcatcga ctagcagcac ccgttgacca gatttggcaa
    22381 aaacaacggc aatgtttgct gcagtggtcg atttcccttc tccaggtcct gatgaagtca
    22441 caacaatcgt tttaatttgc tgaccagctg cggaggcaaa ctggatattc gtccgaatcg
    22501 tacgatattg ttcagcgatt ggtgaagagg ggtttgtgag tgtgaccaaa ctgactgcat
    22561 gagtttgggc tgctttttgt ttctgtgttc gtgccatttt tctttcctcc tagactcttg
    22621 agcggcttct gcgtgatggc gctgttttat cgtttgtagc ttttggttgt gtcggagctg
    22681 gtcctttttt acggatacca gcattcaatt ctttcgctga catttctggt acagtcccta
    22741 agatcgggaa gcccaatgta tccgtcacat atttttcatc ttttactgtc cggtcgagta
    22801 attcaaataa gaaagctaga cctacgccta ccatcattcc taacactaag ccaatcacta
    22861 gattcaattt attatttggc gaaacaggtg tcgtattggc aactgcacct gagatgatcg
    22921 agattttatc gacattcatc acgtctttgg cgttctcttg gaatacttgt gacgttgtat
    22981 tagcgatttg ttgagccgtc actgcatttg tgcttgttgc ttggatcgag aacatttgtg
    23041 aattttgtga ctgattgacg gagatggcat ctttgatttg tccagcagtc atttttacat
    23101 tgtcttctgt ttctaaacga tctttgactt catttagtac tagatcactg acgatcatat
    23161 ccttatacgt attgatcatt tgcaggttcg tattgacatc attgacattt gcagtatctg
    23221 attgcggcag tgtcacgatc aattgggctt gtgagctgta ttttggcgtc aagatgaaaa
    23281 acgtcacgat gcctgagatg gccaagccag caaacattga gatgaggatt gttatcacgt
    23341 gtttgcgtaa tgtttcaaaa atttctttca agctgattgt ctcttccatt ttttctccac
    23401 tttcctattt attctctaga ttcatttgat cttttaattc ttttttcaca cgatcaagtt
    23461 cttgatcgct gactttttgg tatgagatat tgtcttgcat gaagccttct ccttgcagct
    23521 gatcttgctt gatcgtacca aatgcatccc gataatctaa agcaatcttt ttcatttcat
    23581 caaagctcat gtctgttttc atattcgact ctagagcatt caaaacggat tgatagttgc
    23641 ttacaccgtt cagactcaac acttttttcg ctacaccttc tacgatcttt ctttgacgtt
    23701 cttgtctgcc gtagtcacca tttggatctt cataacgcat acgagaataa gacaatgcct
    23761 gatccccgtt caatgtgatt ttaccgatcg taaaatcata gccatcttgt gagaaggtga
    23821 ggtcattatt gacttcgata ccaccaactg catcaacgag ttcttttaga cctttcatat
    23881 tgattgaaac atagtgatcg attgggatgt ctacatattt ttgtacggta tccatcgcca
    23941 tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc ttttgtgtca tgtccaacga
    24001 tgtcgacata cgtatcacga ggaatgctga caatcgttgt ctgtttgtct tcaggactga
    24061 ctgtggcaac catcatcgta tctgatcttc cttgatcgac ccgacctaaa tctcccgtat
    24121 caatccccat caatagaaca gagaaagatt cttgtttgtc cagatcaact ggttttgttc
    24181 gttctccttg ggttcgttcc actgattcgt aagttttttc aatagaatgt gagagatcta
    24241 gatataattt tgctcctact cctacgattg ctaatactaa tacgacaaga attgctaaaa
    24301 taattagaaa tatctttttc cctcttgatc ttcttcttcg ttttgccata gtttcctccg
    24361 tttgctagcc ttgcttgaat tctcgcttat taatagctcg tattgctata gcccatttgc
    24421 tgagcgactg tttctgcttt tgacgcgtat gcggctctta ttttttcatt ccggatctgt
    24481 tcgatgagac tgagtgcggt caaatattga tcgtaagtag cagtagctgt tacttgaccg
    24541 tctttcagca tcgtatcgaa tagctgatct agttgcgtga cttgcgcgat ctgagtatca
    24601 gcgaatccta gatattgcat aacaaccgtt ttccaaggac tatctttaaa aaagctcata
    24661 ttttcacgta cacgagcgac atctgcactt gtcacctttt ctttgatgac gacatcgtct
    24721 acagcttgct gccagtttga tacattttta gtaaataaat cgttgacagc tgtctggatc
    24781 ttcagttttg attctgcatc ctctaactgc gttaagactg ctttcttttc ttcttggatc
    24841 gcagcagctt ctttaggtaa ttctgattcc ttgatgtcat aatcatctgc cgaaagtttg
    24901 atagaagata gtttatgtga caaatttgaa actgtttctt ctgttatttc aggatttagg
    24961 aaaatttttt cttttgtact gtacagctta tctacttctt ttgataaaga ggtcacggtg
    25021 tctttttgat tggtgatggc agattctgcg taattgatcc gttgatgatg ttgccgctga
    25081 ttattgaaat ggtatccaaa caagcaaatc acaatagcgc ctgtgcctac cgccacagta
    25141 gccaataact tgacgaacgg cttttccata gattcactcc taaatattga tgatagataa
    25201 ataaagatgt gattcattcc catcagaatg agcgcttttt attttaaaag cataattaaa
    25261 ctaatatatt catagaaata tttataaaca ggttttccct atataatcat accacaaaaa
    25321 gttttcttga ctagggaaat tcctaattta aaagtaaata aaacagtaac aagatcatga
    25381 ttaatcgttt tcatttttat ttcctctctt ttcatgagca ttttgaaaat tcgaacaaag
    25441 aataaaataa caaaaattat taaaatagaa ttaataacag ctatttatat ttataacaaa
    25501 aaaacagaga cacagcacgt ttcgtgcatg ctctctgttt ttcaaacaaa agactatttt
    25561 gtgatgattt gatccaagct tgctaatgac aaagccatgg cagctatttg actaagttgt
    25621 gttagtcggt tcttacgtac tgctggttca tcagccatga ccatcgtttg atcaaaataa
    25681 ttttcgatca gtggacgaag agcgacaagc tggtcgtagt tttcgtggat cgtttggtca
    25741 gcaaaacttg ctttcattgc ttctactgct ttgtgtaatt ctttctctgc gtcgttttcg
    25801 aacagctcag gatctacttc tgattgtttt tcaagctgcg cttttttcgc taagttcacg
    25861 acacgtgtca aagcttccat tgaaggtttg aagtcttcat ctgcaagatg ttgtttgaac
    25921 aattgtgctg ctgcaaatac ttttgtcaga tctttttgtt ccgcattcaa aacagcatcg
    25981 atgacatcat gacgaatatt cttcgttgtc aataattggc gcagacggcc tttgataaag
    26041 tcgatcactt gttgttgccc ttctgataaa ccaatgccga aacgatccgt atctttactg
    26101 ataacatcgc taatcgtttc ttgcagaaca gacaaaggaa aggcccattt tttcgattcg
    26161 atgatccgaa cgacaccgta tgcttggcga cgtaaggcat aaggatcgtt tgaaccgctt
    26221 gggatcatcc caacagcaaa gaaagaaaag atgctatcta atttatctgc aatcgctaaa
    26281 acagaaccta ctgtagactg aggtagctct ccttcactgg atataggcat gtaatgctca
    26341 cgaatcgctg tcgctacagc aggtttctcg cctttcagca gagcatattt ttcgcccatg
    26401 atcccttgca gttcaggaaa ttcccctacc atgttggtta ctagatcaaa tttatagatt
    26461 tcagcagctc gttgcagatc catcaattct tcctcagata gtcctacttg ttcgccaatg
    26521 atacgggcaa tgacttgtac ccgttgcatt ttctcataga tactaccgat tttttcatgg
    26581 aaagtaacag tttttagttt ttcaacgtat tggtcgattg aagctttctt gtcttcatta
    26641 taaaagaatt ccgcatcttc taaacgagcc gtcaatactt tttgattgcc tcgaaccaca
    26701 ttttccaatt ttacccgatc accattacga gcagaaataa aatgaggaag cagcatacct
    26761 tgatcattcc gtacctcgaa ataacgttga tgttctttca tagaagtgac tagtacttct
    26821 tcaggaacag aaagatattt ttcttcgaag tcgccagaaa agacagttgg atattcgact
    26881 aaattgttga cttcttctag taaatcttcg tctagatcaa taacccaatg atttttttcg
    26941 gctaattcat tggcttgtgt aacgatcatt tcttgtcttt tttgcggaga cgcaatgacg
    27001 aattgttcat tcaatttttc ttcatattct tttgcatgcg caaatgtcac atcttcgcct
    27061 aagaaacgat gacctcgaga ggctctgcct gttttgacat ccaagatttc gaatgggatt
    27121 acttcgtcat ctaacaatgc cacgatccag tgaatagggc ggatatattc aaaatcgtat
    27181 tctgcccaat gcatcgttac agggaaagtc aggcttttaa tgacatctgc taaacctgtc
    27241 aatacttctt tggattcctt accatgagtg tatttcgtta catagacata ttccacacct
    27301 tttaattctc taaaagtgat agcgtctgtc gtcagacctt gtccgcggac aaatccttcc
    27361 gctgctttcg tccagtttcc atctgcgtcc aaagcaattt ttttggctgg tcctttgact
    27421 tcttcttcac tgtccgcttg tcgttctgga atgtttgtta ctttgattgc taaacggcgc
    27481 ggtgtggaaa atgtttcgat cgtatcataa gtcaattggt tttcatctaa aaattttgct
    27541 gttttttctt ctaattgttt catgcttggt gtgacaacat gagcaggcat ttcttccagc
    27601 ccgatttcaa gtaataagtc ttttgccatc ttattcagcc tccttcacgt tttctggatt
    27661 tgctgtgttt ttattcaaca aagggaatcc taatttttcg cgttctgcca caaagatttt
    27721 ggctacagca cgagccatgt tgcggatgcg agaaagataa ccggcacgct ctgttacgga
    27781 tactgcaccg cgggcgtcta ataagttgaa tgtatggcta catttcaaga tataatcata
    27841 cgccggatga acaagatttt catcgataca gcgtttagct tctttttcaa aacgatcaaa
    27901 gttgcttagt agcaagtctt ggtcgcttac ttcaaaagaa tatttagaat gttcatattc
    27961 tggttgtttg aagatctcgc catatttcac gccatcagcc cattctaaat catagacact
    28021 ttcaacttct tggatataag aagcaagacg ttctaatcca taagtgatct cagaagttac
    28081 tggtttacaa ggcagtccac caacttgttg gaagtacgtg aattgtgtga tttccattcc
    28141 gtcaagccat acttcccaac ccacaccggc acagcccatg gatgggttct cccagttgtc
    28201 ttcaacaaaa cggatgtcgt gttctagtgg atcgattcct aaaaggcgca agctttctaa
    28261 ataaagctct tggatattct caggtgaagg tttcatcacg acttggaatt gatgatgctg
    28321 atacaaacgg tttgggtttt cgccataacg tccatcagct ggtcggcgag atggctctac
    28381 gtaagcagca ttccatggtt ctggtccgat tgcccgtaaa aaggtatatg ggctcatcgt
    28441 acctgctcct ttttctgtgt catacgcttg catcagcatg cagccgtttg atgaccaaaa
    28501 tttttgtaat gttaaaatca tatcttggac ggtcaatttt ttactcatct gttttgcccc
    28561 tttcaatttt aaagcgttgc tgtcaaataa gaatacagga aagaaaacga caaaaaatcc
    28621 ctatgtctga ttacagacat agggacgaat aattcgcggt tccaccctac ttccgaataa
    28681 agattcggca gcttcattta tcgtactcgc aagtgcctct tcaaaaatcg tttgggtccg
    28741 gctcccactc tcccgaactc gctaaaccac gtgtttttta ctgatcttgg tcaatgtacg
    28801 tattcatttt gcagttttct tgtttctata atagatcctc tcagtgaacg atctatttga
    28861 tctacggtta ataatagacg gaactccctg tgtttgtcaa atggttttga ttttttgttg
    28921 tatgtttcat ttactattct tcttttttag gtagatcttt ttcagacatg tctttctctg
    28981 acgtctttaa tacgtgtgcc caagacttca tttgatcaat gaactttttg cttttcaaat
    29041 ggataccgac atattcatca tacagctggt cgatgagttc acggattgct tgtttggttt
    29101 cttctttcag atggattcca ctgattttat cataagaaat agctgaaaac atccgaacga
    29161 aataaacagc tcgaggatct gcatgataac ggtgatcatc catcgaccag tgtctttcgc
    29221 atatcacacc accatatttg gaagaatagt caaattttcc ttttgtttcc ccacatacga
    29281 cgcagtgtgt ccagattggt gcaattccga agcgttgcag tatttggact tcaaaaatat
    29341 tcgtgatgat ttctgcatca tctccttggt ccatcagttg cagtgcttgt tgcgtaaact
    29401 ggaacaaatg cgggtcgtac tgatgatctt cgattgccgc atctactaga ttcaataaat
    29461 atgtgccata gccgctcaaa aagatgtctt cttggatgtg tcgaaacgcg tggacatctt
    29521 tgacactgct taaaaatgac aaaccttcac ttcgaaaatt cccgatataa actgcttcag
    29581 taaaagggag caaagccgga gctaagggct gattttgccg atggatccct ttgatataaa
    29641 acatcatttt gccagaagat tccgtaaaga ttttgactaa tttatctttt tctttgtaat
    29701 tacggctaga taaaatcaag ccttttgatt ctgcaagctg gctcatattc tccctcctaa
    29761 aaaataaatc tggaaaagca gattctgccg atccagatca tttatgttgc gatcttccgc
    29821 agcttttctc aacacgcgga tttttatcca gtttatttct atacctaact tacaagaagc
    29881 tcagaatatc aatattcgtc ttttcggtag ccgaagtctt gcagatagac ttttttgtct
    29941 cgccagtctt tttgaacttt gacccaaagt tctagaaaga ctttgtctcc taaaagattt
    30001 tcgatatctt ttctcgcttt cgttcctact tgcttcagca tttttccgcc tttgccgata
    30061 atgatccctt tttggctatc tctttcgaca ataatcgtag cctggatatg gattttatca
    30121 ttctcgtttc gcttcatcga atcaacaacg actgcgacag aatgaggcac ttcgtctcgt
    30181 gttaaaaaca agactttctc acggatcaat tcagaaacga tgaagtattc aggatgatca
    30241 gtcacttgat catctggaaa atattgtggg ccctctggca tttcactgac taatacatcc
    30301 atcaaacgtt cgacgttgtt tccttctgta gcagaaattg gaatcacttg tgcaaacggc
    30361 atctgcgttg aataatcttc gatgattccc aacaactcgt ctggatggat tttatcgatt
    30421 ttattgataa tcagataaac aggagaatgc acgtttttta gacgttcaat aataaaatcg
    30481 tctccccgac cgcgtttttg atcagcgctg atcataaata agaccgcgtc cacttctttc
    30541 aacgcactgt aagctgcttc taccataaaa tctcctaaac gatgctttgg tttatggatt
    30601 cctggtgtgt caataaaaat caactgagct tctggtgtag tatatactcc ttggatttta
    30661 tttctagttg tctgcgcctt atcactcatg atggcaattt tttgacccac gattcgattc
    30721 aataatgtgg attttcctac attcgggcgg ccgacgattg cgacaaaccc tgatttatgt
    30781 tctggcatat acgttctcct ttttaattaa acaacaaatg atatatttta gggacaaata
    30841 aaatcaaccc aacgatgacg gaaaaacaag cggtcactaa aactgctcca gctgccatat
    30901 ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc acgacatttt
    30961 caaaaacagt attgatgatc tctactatcc atacgagaaa aacagctaaa agcagccata
    31021 accattctat ccgatcaagc tggaaaatca cgcccataaa caaagcgagg attccgaacg
    31081 caacatgttt cctcatgttg cgttcttcct caaaaacggt tttgactcct tggacggcaa
    31141 attccacgga catcaggaaa tgtttgtttt tctctacgtt tttatctttt aagtccatag
    31201 gcatctaaga tctctttctg taaaccaaac atttctttct catcctctgg tgtcatatgg
    31261 tcatacccgt tgatatgcaa gaatccatga accgctaaaa agccaagttc ccgatcatac
    31321 gaatggccgt aagcttctgc ttgctctgca gcacgttcag tagagatcat gatgtctccc
    31381 agtacacgag gcaatggatt ctctccatct tcaaaaatca ctgggatttc gtcttcgccc
    31441 tcttcttcca aggcaaaact gatgacgtct gtcggtgcat cttttccacg gtaatcacga
    31501 ttgattactt ggatcgctgc attgtccatg aaagtaacag acatttctgt ttcttctggc
    31561 aactctaaat atgatgctgc aaattgcagc aagtcgtcga tttctttcat tttctcttct
    31621 gagacttggt ttgtttcatc catgaaagta atatccatta tacatcgctt ctttctattc
    31681 ttcttgttct tcttttatct tctcaacttc cgatttcatt ttaggatatt tgatccgaga
    31741 atggaaggta ctgcttaaac cgctgatcaa tgatttttct actttacgga tttcttttaa
    31801 cgtcaatcca ctgtcgtcca attgtccatc ggaaatacgc tcttcaataa gattatggac
    31861 aaaactttcg attttttcgc tcgttggatg gtccattgct cgtactgccg cttcacaact
    31921 gtcagctata ttgacgattc ctgcttcacg gctttgaggt tttggtccag gataacggaa
    31981 ttgctcttcc gtgacttccg ggttgcgttc ctttgcctta acgtagaaat aacgcatcag
    32041 cgtggttcca tgatgctgtc tgcaaatatc gatcaccatt tgcggcatat tataatcttc
    32101 caatattttg gcaccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa
    32161 taagaaattg tgcggatttt ccgctcctga aggcaagttc tcaacgaaaa agctggcgtg
    32221 tttgatcttg ccgatatcat gataatagca agctactcga gttagtaaag aacgtccacc
    32281 gatttctgct acagcattag cacttagatt ggctaccatc atgctatggt gatacgtgcc
    32341 tggtgcttct tccagcaact gtttcaataa aggatgatta ggattgctga gttcatttaa
    32401 tacgatcaca ctatcatcat tgaccatcaa ttcaatatag gggtgcagac ctatcgtcag
    32461 caagaatgag aacagtgcac ctgtgaatcc gcatactagc gttaaccaag tcgttgcatc
    32521 cccaaagctc attccttggt agataatcaa aatgacatcc atcatcacag gaaaaataat
    32581 gacccacata gcagcggata acccttgttc tgagatccgt ttgcgtctga cgatcgttgc
    32641 catgagacca gaaaatacat aggacatcaa aataactgtc agaaagttcg tgccaatcga
    32701 atcataaaaa atgaaaagtg cggatactgc ttgaaaaata ccagccatga tccctgctct
    32761 tcgattcaag aaaaagctta aaacaagtgg agcaaaagcc gcggggtaaa acagcggaat
    32821 ataagcagct tgttctgttt ggaagagttg gaaaaatttc attagtacga cgcttactga
    32881 cattgccgtc acgtagaaaa gtacgtattt tgttctttct cctgcatttt ggaattgaag
    32941 tgaattataa atcagtacac cgatttgaag caaaacagct aaaaccatgg caatgagtgg
    33001 gaaaatagaa gtcgtttgat tcgtcaatcc tagtaattcc aattttttca tcgctgaagc
    33061 atcaatttga tttccttcgc gaacaataat ttctccttgg aagatcatca ctggttgtac
    33121 agcttctcgt gcagattgtt ttaattcttc tgtctttttc tcattcaata catcattaac
    33181 tacaatgcct tgatccacta agtagcgaat catttgttgc tgctctgttg tgacattcaa
    33241 atattgaatc tgatcttctg cttcttgttt aaaagcttcc aattcgcttt cacgaacttg
    33301 tttactcatc tgttcgtcaa tgagcttcaa actttcatcc ctaaccgtgt gaatttccgt
    33361 ttcagtcatt tcaaatatcg tcgaataaaa attgttcggc agtttttggt aaaacgcaac
    33421 gttttctgca ttcacgtttt caaaattttt cttcagtgta gcaatccgct cttctgtagt
    33481 aggtttcggg atattttcat cttccttcgc cttatctacc ttgtcattat aagatttgtt
    33541 gatggtatca ttcgtcttat cgatcaattc gaataattgc ttgatccgat tgttctggtc
    33601 atctgccaga tctttttgat aagtatattc tggagtcact gcttctgctg ccagttttct
    33661 tctttgttcg gtttcttccg tgttctcaat cgttttattc gcacggatac tctcttctgc
    33721 tacttggcct tctctgaaat cattggcctt ttgtttgaca ctactaaatg taacgccgca
    33781 gacaatgata aaaaacaaga acagaaggaa aggcaatgct gcctttccga gttttttttg
    33841 tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga
    33901 tgaagatccg ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg
    33961 tcacttgctt caaaattaac aaaatcgatt tttttgattt gctttaatgt tcgttctgca
    34021 tgaacaagac cacttgtcgt cccacgaggc aagtctatct gacttgtatc cccattgaca
    34081 atcatttttg attgataacc gagacgggtc aagaacattt tcatctgagc gactgtcgtg
    34141 ttttgtgctt cgtccaagat aacgaatgca tcatccaatg tccgcccccg catgtaagct
    34201 aaaggtgcga tttcgatcac gcctcgttcc atcaatcggt tcgtatgatc caatccaaaa
    34261 atttggtata aagcatcata aacaggccgc agataaggat ccactttttc tttcagatcc
    34321 ccgggtagaa agcctaggtt ttcccctgcc tccacagcgg gccgcgtcaa aatgattttt
    34381 tgcacttcgc ctttttttag ggcagcaatt gctaatacta cggcaagaaa cgttttccct
    34441 gttccggctg gccctacacc aaatacgatg tcatgtttgg ctaccgattc aacgtatttc
    34501 ttttgaccgc tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc
    34561 tcatacatct caacaaaata atctaatgta cctttttgcc ccatcttcaa tgccgtcaca
    34621 acatctggcg tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt ccgaatgact
    34681 gaggcagcca aggaaatctg ggattgctca ccaatgatct gaatgatttc tccacgtgta
    34741 tggatggttg tttccgttgt ttcctcgatc agttttatat gcttgtcgtg tgcacctaaa
    34801 agcatgctga catcatcagc agctgtcaat ctaatctcta aagaactaga ctcttccgtc
    34861 aaaaatagac atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa
    34921 actatatagc aaataagaaa aggttgtaaa agagtcgttt aaccatacgt accaagaaac
    34981 gttaaaaaat agccgtttcc attcgctaaa ggtctcgttt tgataaaaag aagctgagaa
    35041 tgtaagttat atgaaagtgg agaacaaaat attccgtttc attcagcagg tattctctag
    35101 gtatttttct tgtttcaaaa aaagaaaaca aaatagttaa aatattcttg catttcaaaa
    35161 aaaacaggtg tattattacg tcaacaaata aacatacagc aaagcgtcga ggaaaacagt
    35221 aggctattca acgatttatt cccagtgagt catgtaagtg agaaatgaca ataaatcgaa
    35281 tagacgaaca tcatttccga gtcaatgtcg aaaacgtaga tcattgtggc tgcctccatt
    35341 atcaggctag cggataactt tatcaaaaag cgtccgtgaa tgaagaagct tttttaagcg
    35401 aaaaaaggtg gtaccgttct agaatctaga gcccttttcc caagtggaaa agggcttttt
    35461 ttatatttta aggtggtggt tttcatggaa tctgatatta gtggcattac taattgctgc
    35521 aacgtttata tcgtcaatca aaatgataat tattggagga aaaatttatg gaagaaacaa
    35581 aaacttattc atggaaacaa ctattgatgg tcagttcact tatcttcggg atgttcttcg
    35641 ggtcaggtaa tctaatcttt cctgtccact taggtcaaat ggctggcgca aactgggtca
    35701 gtgctggcgt aggatttgcc atctcgggcg ctcttttccc attactagct atcttagctg
    35761 tagtagtgac aaaaagtgat ggtctgtatg acttagctaa acctgtcgga aaatggtatg
    35821 ctgccttgtt cttaatcctt gttcatttga cgatcggacc atttttcggt acaccacgga
    35881 cagctgctac tgcttttgaa atggctgcta aaccattttt gcctgaaaaa tataattcat
    35941 taggtatgtt tttattctct gctcttttct ttttacttgc ttatttcctt actgtccatc
    36001 ccacacggtt ggtcaaatac gtcggaaagt atctaaactc cttgtttctt gcattgttag
    36061 caatcatttt tgtcgtttct ttattccacc caatgggaaa tctaaatcaa tctgctgcag
    36121 atacttacca aacaaacgct ttaacaaatg gtgtcctaga aggttataac acggttgatg
    36181 cagtagcctt gcttgcctta agtgtcactt ttgtccatgc agtccgtgac cttggtttca
    36241 aagataaaaa agtcacagaa ttaacagcaa aagctggtag cctgagcgtt gcgctagaag
    36301 tcatgatcta ttttggtctt gttcttctcg gtgcgatgag tttgaataag atgtctttat
    36361 cagaaaatgg cggaactgca ctttctcaga tcactaattt ttatttaggt aacttcggcg
    36421 ctagcttctt aggagtgatg gtcacattag gtgtcttcac taccgctatg ggactcgttg
    36481 tttcttttgc gcaagatttc cataaactgt tccctaaagt cagttatatg acatggctgc
    36541 ggttaacgac tttcgtctct tttgtcgttg caaatgctgg tttagacaat atcatccaat
    36601 ggtctttacc agtattgatg cttttgtatc cattgtcgct tgcgttgatc ttgctttcct
    36661 taacagcaaa atttttccaa aagacaccat ttgtctatca agtaacgatg ttgtttgctg
    36721 ctgttcctgc cgtattggat atgctggcaa attcgcctgc tttagtgagt caacaaagag
    36781 ttgtagcaag catgctcgaa ttttatcatc atcacgttcc ttttgctgca ttaggacttg
    36841 gctggatggt cccaacgtta cttggttacg ctggcagcct actgttttat tacgcctatc
    36901 gcctatcagg gtataaacaa gaagcaaatg aactaccaga agaataacca aatcaaacac
    36961 aatttaacat aagaaatatt aaacaggaaa aaaccagcta gatgaaagaa tcctattctt
    37021 tcgtctagct atttttttgt tctctttttt acgcgcacaa aaaaactgcg gacaatgatt
    37081 gtccgcagtt taaatgataa aattaagatt gtaatagttc tttcacaatg gcatttactt
    37141 ggtttccatc tgcttttcct ttgactttag gcatgatcgc acccatgact ttgccaaatt
    37201 ctttcattga tgaagcacca gtttgtgtga ttgcttcttg aacaagttgt cgaatttctt
    37261 cgtcggaaag ttgttccggc atgtattttt cgactatcac aatttcactt tttacttttt
    37321 cagctaaatc gtcacgtcct gcttcttcaa actcatgcaa agaatcacgt cgctgtttca
    37381 tttcgcgaga caacaccgtt aattcttctt cttctgtcaa gtcgtgacct tctttaatct
    37441 gctcgttttg tatagatgac ttgatcattc ggatgacttg taaagattcc ttatctttcg
    37501 ctttcatcgc tgttttcatg tcatcgttta atctacttag tagtgacaca ctaccaactc
    37561 caattcatca cagaactaga attttttgcg ttttctagct gcttcagatt ttttcttgcg
    37621 tcttacactt ggtttttcgt agaattcacg tttacgagat tcttgtaaag tacccgcttt
    37681 tgacacggaa cgtttgaagc gacgaagagc atcgtcaagt gattcgtttt tgcgaacgac
    37741 tgtttttgac atgttaattc cctccctccg agcttaaaaa gtaaccgtaa ttgttattga
    37801 aatatatctc aaaaaacaag actgtcctgt aaatattata tctaatgctg ttttgaccgt
    37861 caaggaaatc ttgctttttt ttaaaattat caaatgttat tttatttaaa tccttattga
    37921 caattttcac atcttccgaa aatctcgaat ctatgccctt cgatcgtaca tccaccgagc
    37981 tgttcttcga aataattcat tggacacata tggatttctt tcgttgttcc gcacaccgta
    38041 cagataaagt gatggtgatg gccgatttcc ccttgacagc aatgaaaacg aaacttcatc
    38101 tctccattta aatcggtttc ttccagtaaa tcgatttctg caaaatcatg gagatttcga
    38161 taaatcgtat cgtaacttac tcctttgaat tcctgattca tatagtcaaa aacttcttta
    38221 gcggatacgt agcgattgcg cttcactaaa taagttaaca atgcttctct ttttttagta
    38281 tatttaaaac cgttattttt caatgtttct agtgattgtt caatcaatgt ctgttttttc
    38341 atgacttatt cctccaaatc aaaatgatta cgaagtacac attctagagt atggtataat
    38401 aaacataaaa aaaatgcaag tgaggtttta tccatgtctt ccgattctac agaaatcgtt
    38461 actttttttg tcatctctga ctctgctgga gaaactgcga caaaattagc gcaagccact
    38521 atggcccaat atcctaccgt tgagttcaat ttattccgca gaacatttgt gacagatgag
    38581 aaaacattga tgcaggcact gcaagatgca ctaaaagaaa aagcccttgt cctgcacaca
    38641 ttgatcaatc aagaattgat cgagatgacg cgttcttttt gtgaagaaca tcaattattt
    38701 agttttgatg tgatgactcc gccgatatca gaaatcgagc gcttaactgg tatcaagccc
    38761 attcggcaac ctggcgccct tcatttattg aatgagaact atttcaaacg gatcaaagca
    38821 atggaatttg ctgtaaaata cgatgacggg aaagatcctc gtgggtttct agaagcagat
    38881 gtcgttttgc taggcgtatc cagaacttcc aaaacgcctt tgagcttgtt tttagctaac
    38941 aaaaatttaa aggttgccaa tctacctttg atcccacaag cacatattcc caaacagctt
    39001 tgggagatcg atcctaaaaa aatcgtggga ctgacgaata acccagatat tttaaacaat
    39061 atccgtaaag aacggatgcg ctcttacgga ttaaatcctg atactgctta ttccgatatc
    39121 gaaaaaatac gagctgaact cgattttgcg aatgatcttt atgaaaaatt gggctgcgta
    39181 gtaatcaatg tcgcttcttt atctatcgaa gaaacagctt cacttatttt gaatgcttta
    39241 gacctagaag atcatagtta ttatggaact gaaaccaccg aagaaacaga ctaacagttt
    39301 ttcttgtctt tttcttaaaa aaactggact gtgtcagcga agaagattcg ttgatacagt
    39361 ccagtttgtc tctatttgca cctatcgtta ttgatgttcc acatgtttga gcgtctggtt
    39421 caaaatatcc agtacgtgag aatcatcaag actatacaaa atcgcttttc cctcacgatg
    39481 ggacttgacg acatggtttt ctcttaataa acgcagttga tgcgatacag cagattgttc
    39541 catttctact gcttgggtga ttgctgtcac attttgttcc ccgtctttca gatacagcag
    39601 aattttgaga cgagtggggt cactcaacat tttgaatagt tggctgactt tctctacttt
    39661 tgttttttgc gtgtctggca atccattcac ctccaacttg aaaaaagact gacttctaaa
    39721 gtttaccact ttttccatca gtctcaagtt tttttattct tctgtcattt ttttgataat
    39781 cgcttcataa gcggctactt tttcagctgc gtcttctgaa gagtttcctt ttacaccgat
    39841 ataaaatttg attttcggct cagttcctga aggacggata gcgatccagc cattttcttc
    39901 cagcgtatac ttcaatacgt cagatggcgg tgtcgttagt ttttctacgt ttccttcacg
    39961 gtctgttctc gtcaactctt tgaagtcttc tgtttggatg acttttgctc cggcaaattc
    40021 tgtcggtgct tcttcacgga atttttccat caaagcagag atttttgcag aaccttcttg
    40081 tccgctcatt gtcacagaaa tcgttttttc tgcgtagtag ccatatgttt caaaaatcgt
    40141 ttgcaagccg tcatacaacg ttttgccttg ttttttatag taagctgcta cttctgctaa
    40201 taagagcaat gcttgaatcg cgtctttgtc gcgaacgaaa ggtttaacta aataaccgta
    40261 actttcttcg aatccgaaca taaaagtatg ggaatgatct tcttcaaatt gttgaatttt
    40321 ttcagcgatg aatttgaatc ctgtcaatac attgaacatg gtagccccat agtgttccgc
    40381 aattgctgta ggcaattcgc tagaaacgat tgatttcaat accgctgcat tttcaggtaa
    40441 agtgcctcgc gattggtgtg cttccaaaat atattgagca aacagggctc cgatttggtt
    40501 ccctgtcaat acttcatagg agccatttgg caaacgaacg gctgcgccta aacggtctgc
    40561 atcaggatct gttgcaatca gtaagtcagc gccttctttc tcgccaagtc gcatcgcata
    40621 ctcgaaagca gagtgttctt ctggattcgg tgatttgact gtagtgaaat ccgggtctgc
    40681 gacagcttgt tctggaacta gtacgaattg ttcgaatcca gcttgtttca acgcacgttc
    40741 gcccaacatt tttcctgttc cgtgaagtgg tgtgtacaca agtttcaaat ctttacccat
    40801 tgtttccacc aattcatgat caatcgttac tgctttgatt tcttctagat aagcggcatc
    40861 cacttcttct ccgatgatcg tgatcaatcc gctatgttca gcttcgtcgt cagataatac
    40921 ttcaactttt aacggattgc tgacttcacg gacatatttc gtcaatgcat ctgcgtctgc
    40981 aggaggcatt tgtcccccat cttctccgta gactttatat ccgttgtaag ctgctggatt
    41041 gtgggatgca gttaccatga ttcccgcaaa cgtcttcaaa tgacggactg caaaagaaag
    41101 ctctggtgtt ggacgtaaac tttcgaatac ataagaaggg atattgtgat gtgctaatgt
    41161 tttggctgct tccatggcaa attctggtga ttgatggcgt gagtcataag cgatagcgac
    41221 acctcggcgt tttgtttctt caccttgtgt atccatgaaa cgtgcaagtc cttctgttgc
    41281 ttgtcttacc gtatagatgt tcattcgatt gatcccaggt cctaagattc cccgcatgcc
    41341 tgcagtaccg aattccaacg gtgcataaaa agcatcttca agtttttcag gatcttcacc
    41401 cagatcaatt aattgttttt ttaagttttc ttctaaattt tcttcatgta cccattgttc
    41461 atacacttgt tcccaagaca tgaaaataac acctctttct gaaatatggt tcttaataag
    41521 tatagcattc acttattaga tatgaaagta cgaggtctaa aaaacgctta aatcaagaaa
    41581 ggattctttt atcaagcacg gttgcaagta ttcgcataaa tcggattcag tagatttcac
    41641 gcgaaaaaaa agagtaaaag aacaattgaa accgttcttc cactcttttt aaacttatgc
    41701 agttgtttct tctaaagatt ccaagtattt atacacttgt tgtccagcga tcccaccatc
    41761 accaacagca gtagtgattt gtcgcaaatc tttttcacga acatctccga tggcaaaaac
    41821 gcctggtata ttcgtcttca tttcgttgtc tgttggaatc cagccagctt cgttcgtcaa
    41881 ccctgcttct ctaaatggtg cagttaatgg atccagtcca acatagatga atactccgtc
    41941 tgctgccaaa tcaaactcat tgccggtttt gacattacgt gctttcacac ctgtcactac
    42001 catttcattc ccaacgattt cgtcaacgac tgtatcccac ataaaggaaa ttttttcatt
    42061 cgcaaatgca cggtcttgga tgatcttttg tgcacgtaat tcatctctac ggtggacgat
    42121 gactacttca gaagcaaatt gtgtcaaata gatggcttct tctactgctg agtctcctcc
    42181 tccgatcact actagacgtt tgttacggaa aaacgcacca tcacaaactg cacagtaaga
    42241 aacgccacgt ccagcaaatt cttcctcgcc aggtacgcct aatttacggt ggatacagcc
    42301 tgttgcgata atgactgttt ttccttcgta agttttatct tcacaaataa cttctttgtg
    42361 gctgccgtga tccttgatat cttgcacgat tccataagca ttttctgtcc caaatcttga
    42421 aacaccttca tacatcttat aagctagatc aggacccatg attgaatcga aaccaggata
    42481 gttttcgatt tctgccgtat tgttcatttg gccgccaggg gcgccttgtt cgatcatcaa
    42541 tacggataag tttgaacgtg aagcatataa agcagcagtc atcccagcag gacctgcgcc
    42601 aataacgatt acatcataca tatgtagatt ctcctttact taatcaagta agcatacttt
    42661 acagccaatg cgaaaaaaag tctattgttc tgcttgcgtt tctcgaatag ctttattata
    42721 ctcgaaaaat ccgctgaaaa acaatagact tttacaaatc attgatcaat cattcgaaag
    42781 attttctcca tttgaatcaa ttactttttt ataccagttg aatgatttct tcttcgaacg
    42841 ggctaatgtg ccatttcctt cgttgtcacg atcgacataa atgaatccgt accttttctt
    42901 catctcacct gtaccagctg atactaaatc aatacatccc caagtcgtat agcctaatag
    42961 atcgactcca tctaattcta ctgtatcttt cattgcctgg atatgtgctg ccagatagtc
    43021 gattcgatag tcatcttcca cataaccatt ctcatcgggc gtatcgactg cacctaagcc
    43081 gttttctaca ataaatagtg gtttttgata acgatcataa atatcattca tcgttattct
    43141 caagcctaat ggatcaattt gccaacccca ttcacttgct tccagatatg gatttttgac
    43201 agaagcaaag atatttcctg cagtctgttc caagagttcg ttgtcagtag tcgaaactcg
    43261 agaagaataa taagaaaagg agataaaatc aacagtatgt tctttcagca atgcggcatc
    43321 cccaggttcc attttgatct gaatgccatt tcgttccatt tctttcaatg cataagcggg
    43381 atattctcct cgtgactgga catcaatgaa gaaataattt tctcgatctg attttctaga
    43441 agcccaaaca tcttcaggat tgcaagtgta tggataatat gctcctgctg ccagcataca
    43501 gcctactttg ttctccggat ctacttcatg agcaatcttc gttgcgatgg cacttgcaac
    43561 caactcatga tgtgccgctt gatatttgat ttgttcttca ttttctcctt cttcaaaata
    43621 cagccctgcg cccatgaatg gtgcatgaag gatcatattg atctcattga atgtcaacca
    43681 atatttgaca agtcctttat agcgattgaa gatgactcgg cacagatttt cataaaaacc
    43741 aacgagttct ctagaacgcc acgcaccata ttctttgatc aaatgcatcg gacaatcaaa
    43801 atgtgtaatc gtcacaagcg gctcgatccc gtacttttga cattctttaa agacatcctc
    43861 ataaaatttc aaaccagatt catttggctc attttcatcg cccattggaa aaatacgact
    43921 ccaagcaatg gacatccgat aagtcttgaa ccccatttct gcaaacaaag caatatcttc
    43981 cttataatga tgatacatat cgatcgcaat ttttgctgga taaaaatgat ctttatcaaa
    44041 ttcaaacatt ttcttttttc cagtaatgac aggaaaacgg tcttctccga ttggcacaac
    44101 atctacgttc gctaacccac gtccgccttc atcataacca ccctcgcatt gattggctgc
    44161 tgtagcaccg ccccataaaa aatcttttct gaatcccatc atacttcctc cattacatgt
    44221 gtattgcttg acaattctct tgccagttca tcttggactt cgattccttc tgcttttaac
    44281 cggctgattg tttccttgaa ttgcggtaaa tactgctgat gtgcaataaa caactcatcc
    44341 atgatccgtt ttgctgtctt tcctgatcgt ataagcggat tgatcgtaaa cgcttgtaaa
    44401 gctgttccat aatcaccagt cacggctgct tcgatcgtca gcaattccat attcttcatt
    44461 acttgcaacc agcctctttc agcagcagga agggaaccaa acgctacatt tcgtgcgcct
    44521 tgtgcgccaa cataggcagt gacttctact acacaatctg ccggcagatc aggaacagct
    44581 ccatcattct ttgtagacac cacgatctgt gtttctttat ttccatagat cgaagcaatc
    44641 gtttcacagg cagcatctga ataataagca ccacctcgtt tttgcaattg ttcaggttta
    44701 tgatccaatt ctggatcttt gtataattca aacagctctg cttctgtttc tttcacttgt
    44761 tgcgctcttg taccaatcgt ttgatattct tctaaagcat gttccaacat ttcttcttca
    44821 cggtagtaat agcggtggta gccacaaggg atcatgttca tttgttttaa ttgttctttg
    44881 aaaaacggga tgtcaaagat atttttcggc aatccattgt cttcatcgta taaacgatca
    44941 atcaaatctg ctgtgcggtc atttccttta ttatctgcta ctttgtgcca atggaagtga
    45001 tttagaccgg caaacttgta gatcagctct tcttttttct tgccaagcat tgctggttct
    45061 gtcatcgtcg ccattactgg cacattgcat aatccgatca ctttatccca tttgccatat
    45121 ctgataactg cttctgtcac catcccgctt gggttcgcga aattgatcag ccaagcattt
    45181 ggacataaac gcttcatgtc ttcaacgatt gccaaaacca ctggaacggt acgaaaagct
    45241 ttgaatatcc cgccagcacc atttgtttct tgcccaatca tcccataata actagggatc
    45301 cgctcgtctt tgattcgtgc atctaattga ccgacacgaa actgagtcgt aacaaaatcc
    45361 gcatctttca atgcttcttc ccgatccaaa gttgcataga tcttgacttc atacggagaa
    45421 gcatcccaca tacgctgaga catctgactg acgatctcaa gcttttcttt tccttcttct
    45481 acatctacga gccagatttc tcgtatcggt aattcctcat atcttttgat aaatccttcc
    45541 atcaactcgg gggtgtaact gctgcctcca ccaatcgtag cgattttgat tccttttgcc
    45601 atgttctcac tcctttgctt tttacacttt tattatcttt tttgtaaaca aaacaaacaa
    45661 tagaaattcg tttctttcca gtaacattta caaaagttag tgttttctac tggatttttg
    45721 taaatatctt gtcaaaaaat gactttatta agctatataa cataaatccg tttacaaatc
    45781 attttcaaaa aagttataat ttacgtaaaa aaactagaag aaggtatcta catgttatta
    45841 cgtgaaaaaa tcaaagaaat ctccttttct ccttcagaaa atgaagtgat tcagtttctc
    45901 ttaagcgatt ctccagcact tggcgaaatg accatccaag agattgctga gcgatgttat
    45961 gtccacccgt ccacactcat ccgtgtagca aaaaaaatgg gttttgatgg ctgggtcagt
    46021 tttcgtaatg cttttttagc tgaaacagcc tatcttgaag ggaattttca agacgtagat
    46081 gcgaatcttc ctttttccac cacagacggt attatgacga tcgcaaataa aatggcttct
    46141 ttagaacaga tgacgattca agatacactt tctctcattc atcatgacca actgcaaaaa
    46201 gccaaacaac taatgcttca agcaaaaaaa atcgtgattt tcgccagtaa ctcgaatact
    46261 ttgatttctc aagatttcat gctgaaaatg aagcgcatca aaatggatgt cacgattgtc
    46321 accacttttg gtgaatcgtt ctatgaagcg tacaattgca cgagtgatat gtgcgggatt
    46381 ctgatttctt atacgggtga aaacaatgtg atccttcgta ccgcagaaat attgaaacaa
    46441 aatcatgttc caattttagc gttgaccagt atcggtgatt caacgctatc ctcaatgagt
    46501 acagccgctt tgcgacttac gacacgcgaa cgactgtatt caaaaattgg taattttacg
    46561 atcaatacat cgatttgtta tcttctagac gtgttatata gttgtgtttt tgcagaaaat
    46621 tatcaacaga atttagtcca tctaatttct gtaggacaaa aatttgataa acgccctatc
    46681 agctcagcca tcatgaaaga gcctttcaat tttgaaaata aagaagtgga ctaaaaagaa
    46741 tagttaagtc tattccattc tttcacctaa ccgttcaggt cagtgcggtg ataagtgcat
    46801 cgtctggccc gaccgtctcc ccttgatttt ccaaaatatc caaataatcc atgtaattgg
    46861 tgacgattac aggtgtttct aaactgtatc cttccttttc gattttcttt ttgtcgaagc
    46921 ggagcaacag atcgccttgt ttcactcttt ctccttgctg caccaagctt tcaaaatatt
    46981 tcccttccaa ctgaacagta tccaatccga catgaatcag caattccaga cctgtttcag
    47041 aaaccattcc gattgcatgt ttcgtgggga acagggtcat gactgttccg tcaaaagggg
    47101 cacgtacttc tcctttttca gggtagatca agattcctcg acctagtgtt ccttgtgcaa
    47161 aagctgcatc ctttgctgct ttcagtggag cgatttgtcc ttgtatcggt gtttttacaa
    47221 tttctttacc tgatcttttc tgtctgtcat ctgtttcacc tgcttcctcc ttccaaaaga
    47281 aaaaagtcag cgcaaaggat accaccatcg caatggcgat cccaatcaat acattaatca
    47341 catctgttaa atcaccatct ggtctaatat agttgggaat tgtaaagatg cctagtccac
    47401 cttgattcca agaagttaaa ttcattaacc cactatacgc gccccctaac gcaccaccga
    47461 cacaagtaaa aataaatatt tttttacgtg gtaatgtaaa cccataaata gctggctctg
    47521 taatccccac aataccagaa acaatggctg gtattgcaat tgctttgtct tttggatttc
    47581 taagcttaaa gtagagtgcg gtaagtgctg cagtttgtcc aaaagacact actgcaacag
    47641 gtgtcataat attttgccaa ccattcacag aaaattgcat caatcccatt ggtacgattg
    47701 cccaatggag accaaacatc accagaattt gccagaagaa gcctacgatt gctccataaa
    47761 taactggcga aagtgctaaa agtgtttcaa atccagccat taaaatatca gttgcgaatg
    47821 tgaatactgg tccaacgagt aaaaaaccta aaggcattgt aataagaacg gtgaagaatg
    47881 gaacgaagaa attcgcgatc atctcaggta ctaacttttt aattggtttc tgaatttggc
    47941 tagctaacaa cacaataaag ataatgggaa tgacagaaga agcgtagtta cgcgcaatcc
    48001 agggaatgcc gaatactgtt tgataaatag gtgcttcaaa aatagtgcca gaaaatatgg
    48061 tcgttagtgg tgcttctgct gcaccactca aagcttccag cgacaagcta ggattcatta
    48121 atgctgcgcc aagcaacatc cctacgaatt catttacttt aaatttacgg gcagctgtta
    48181 aaccgataat aacaggcata aattggaata cagaatcacc gatattatca aacataacaa
    48241 atgtgccaga accgcggtca aacgcgccta agaagacaag gaatgctgtc agtccacgaa
    48301 tcatcccgcc agcagctaag atagcaagga atggttggaa acagcccgat attccatcaa
    48361 taagcatatc aaacggtctc attttttctg ttgaaacagc ctcatcaaaa gtaaggtttc
    48421 ctgcttttat aacctcttca aacacgactg gcacatgatt cccgatgaca acttgatact
    48481 gccctcccgc tcgcattacc gttacaatac cgtccatatt tttcaagatc tcatcatttg
    48541 ctttttcttc gttttttaat ttgaaacgaa gccttgtgat acaatgtgtc aaaccattga
    48601 tattttcctt cccaccgaca ttttctacga tttttctcgc taattcttgg tattttccca
    48661 tttctattcc ctccaatata ttagtggaag gtttagccaa ctgaatgtca ctacccttat
    48721 ttcatacgtt cttgttaatt tatcctcctt tttttattta tttacaacga ttctcatgca
    48781 ctaaacgcgc agtatggatc gttaaataca ttttttcgtc cgtggacatc tggtagttga
    48841 acgtctcggc taaaaagtct gctattttct caacgcaacc aaacgtatcc ttatattttt
    48901 tttgaataac ctgaaacaat tcctcagaaa cttctcccac ttctgatttt tctccatcta
    48961 ggacgcgtgt cagaaaaaaa cgtaagtgtg tgacgaatcg aaaaaaatgg actgaatctt
    49021 catcaaatgt aatcttatag taatagcgga cgatatttag aatatcctgt attgttatag
    49081 taaacaggta ggtattgttt tgttggcttt gaatgccttg tgcattgact aaatgcaatg
    49141 cgataaatcc ggcttcatct tctgataact tgatgtggta tttatccgag attttcttca
    49201 ctgttttttt cccgatggca tactctttag gaaatagttg tttgatatca tacagcaata
    49261 catttggtac aaatattccc attttgtgac gttcgatagc cgtatacaaa tgatccgtca
    49321 atgcaatata cacagaatca ttcaactgga cttgtagagc ctcttttgta tattcaatga
    49381 tttcacaaga aatttctaag tactcaagag gaatcgcttg tgccaattct tggaactgtt
    49441 gtgaaagccc gtcatttgtc aactgaaatt ttttgtcgat gcatgagtca aaaatcgggt
    49501 cacccacttt ctttttgaat gccaaacctt ttcccatcac gatcatttcc tgatttcttt
    49561 catccctggt tattacgacg ttgttattca gtactttctc aatcagcatg ttttgctcct
    49621 ttccatgata tatagggctg tattcaaaac aaaaagaaaa acccaaaaag ccgtccatgg
    49681 aaccatggga gactcatcag gtttagcttg catcaggcaa tcactatcca agttgtatcg
    49741 ttttctttat attatcgaaa gcaaacgctt ttgtcaacgc tttcttagtg gctattaggc
    49801 acttttgact agctgtcgtc gtctttttta aatcgggtga ctacgccact aacgctcaca
    49861 gatgaagaat ctgggaagct ctgttttatt ttcactctag cgcttaggac aaaatgctac
    49921 tatgctactg tcacaacctc tttttacaca ccactgcttt gcagtttgta catatctgca
    49981 tacaaaccat tttgcgctag aagtgtttca tgattcccac gctctactat atgacctttg
    50041 tctaaaacta aaatcaattc tgcatcccga atcgtggaca gccgatgggc aatcgcaatc
    50101 gttgtccgtc cttgtcgcat ttttgccaat ccttcttgga tcaatccttc ggtttcagta
    50161 tcgatatttg ctgttgcttc atccaatact agtattttag gatctgtcac gattgttcgc
    50221 gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg caccgtgttc gatcactttt
    50281 gagtgatatc cttttggtaa ttcatcgatg aacttgtttg cttgaacaaa ctcagctgca
    50341 tggcgaatct gttcgtctgt gatagctgga ttcatcatcc ggatatttcc tgcaatatct
    50401 ccataaaaca agaaggcatc ttgcaagacc aatcccattt tcttacgtaa ctcttccatt
    50461 ggatattctc gaatgtcatg atcatcaatc aagatttggc cttcataaaa ttcgtaaaac
    50521 cgcatcaata cgttgatgat cgagcttttt ccgcttccgg tgtgccccac taatgcaacg
    50581 gtctcacccg gattagcgac aaaagagata ttgttcaata cattgtgctc tccatcataa
    50641 gaaaagctta cattgcgaaa ctcgatctta cctgaagtaa tcgcttgatc tgctccagga
    50701 ttttgttgcg gtgcatattc ttctgtatcc atgattttca aaatacgact accggcaacc
    50761 atcccatctg taaaggtgct caaaaagtcc atcatttgag acattggatt aaaaaatccc
    50821 tggacatagg tcgtaaatgc atagatcagt ccaacttcta cgggagaatg caacgcatcg
    50881 atcccaaaca aagtcaacgc caacgcaatc gccaatgcat agagaaaatt gatgatcgga
    50941 cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg tgtctaaata ctcgttgttc
    51001 acttgttcaa attctttagc taaacgagct tcttggcgaa actgctggat aatacgcatg
    51061 ccggaaatcg attcattcaa tttcgtgttc agctgactta atttttcccg catatttcgg
    51121 taaatacgag aactgaattt ctggtaatac cagatgacga ttcctaaaat cggcaagaac
    51181 accaaagtcc acaaggcaat ttctccattg atttgaaaca tggcaacgaa agaagaaacg
    51241 actgcaaaga tcccagtcaa taccatcaaa aagacatacc aaaattcaaa tagcgtttct
    51301 gtgtcattcg tcactcgaga aacggtcgat cctgccggtg tctggtcaaa ataacgcata
    51361 cctagtgtat gcagcttttc aaataatctt acccgaacat actgataggt cttcaatgaa
    51421 gccatggaat acaaatacca ttggaaaaac caaatgatac ttttgactat gactccaaac
    51481 aagtacacac cagcaaaaaa caaaataact tgtgtcgtag ctgactgttt agccagatga
    51541 tcatccataa aagtttggat gatccttggc aataggacat tgatcacaga cagggcaaac
    51601 gcaaataaga tagcaacgat gaacgttctt ctaaaaggtt tggcgaactt catcagtcgt
    51661 ttgatgatct tcttctgttc tttaagtgga atcgattttg tccattccga ttgataattt
    51721 tcatccatca ggcctcgcct ccttctatct tcgcttctaa ttgctgtttt tcccacattc
    51781 gttcatacca gccacctaat gcaagcagct cgtggtgggt tcctcgttca atgattttcc
    51841 cttgatcgac aacaatgatt tcttttgcat gcatcacgct gcttaaacgg tgagctgcaa
    51901 tgatggttgt tttctctttt cttatttctt ttaaattatg caagatagct tcttctgttt
    51961 tggcatctac tgcagaaagt gcatcatcta agatcagcaa ttcaggattt ttgatcaatg
    52021 ctcgagcaat cgaaatcctt tgtttctgtc cgccagataa agagactcca cgttctccaa
    52081 ctaaggtatc ataacctttt ggcaattcct tgatttcatt gttgataaat gctaaagaag
    52141 ccgcatgctc tacttcttct tctgaaagtt ttggattaga aaaacggata ttatctcgaa
    52201 tggtcatcga aaacaggaag tgatcctgag gcacataacc gattgatcct aataaagcat
    52261 tcaatgaata ttctttgatc gaacgtccgc caaattggat tttcccttga taatcatcgt
    52321 attcacgcat cagtaatttg atgattgtcg tttttcccgc accagttttc ccaacaatac
    52381 ctaatgtttc tccttcttgg atcgcaaatt tgatttgttc taatgctggt gattcttctt
    52441 tcggatagtt aaaagaggaa atagctacat tcagctcgcc ttttgccggg gtctgcactg
    52501 cacccagttg ttccacgata tgcgtctttt catgcaaaag ttcattaacg cgatcataag
    52561 aagcatttcc tctttccaat acgttgaaca atcggccaat cgcaaacatc ggccaaacaa
    52621 gcataccgat ataactaata aatgagacca tctgcccaat cgagatcgtg ccttccaaga
    52681 catactttcc acctaaaata attgttgcaa cataagacag accgataatc aaggtaatga
    52741 atggatcaaa taaggagtct aaaaaattga ctcgtttgtt tttaataatt gcatcttcaa
    52801 tcttttcttc aaaatccgcg agatcttctt tttcttgtcc aaatgtttta atgactttga
    52861 tcccagtgat actttcttgt gtcttatcat tgatactaga aaaagcttct tgcgaatcac
    52921 gaaaagcatc gtggagtttt gaccccagca ctcgtgaagt aacggctagc aaaggcagag
    52981 gaatcagcgc aatcagtgtt aagcgccaat caataaacaa aatcatcgca atgatcgttg
    53041 ttccgcccgt aatcactgaa tctgcaaagg tcaagattcc agctcctgct acattctgga
    53101 ttgcattcaa atcattcgtt gcatgagcca tcaaatctcc agtccgatat ttttggtaaa
    53161 agagattgtc catttctgta aaatgtttga atagctgctg ccgaagttct ttctccaacc
    53221 gagctgcact tccccaaata ttcgtccgcc agatataacg aaaaagatat tgtaagaaag
    53281 ctgcaaggat caatagtaag atccaccaag aaatgatctt tattgataaa ttcttatcgg
    53341 cgatctcatc aatgatgatc ccaatcactt ttggaggaac taactgtacc aaggccactg
    53401 cgaataggga gaaaatccca attaaataat gtttcttttc ctgacgaaaa aaccaaccta
    53461 attttctaaa aatcgacatt ttactcctcc tttgtcttta tcaagagcat aggattgtct
    53521 gtccttgtct tttttgctga atagtctgaa tagtctaaca aattacgaaa taaaaaacgg
    53581 actgaaaaaa tcagcccgca aaaagtcttt gggtcagaac ctattttttc ccttggttct
    53641 tcatttgctg catcatctgg cgaattttct tctcagatgg tttttgcccc attgacatca
    53701 tcattgatcg caacatgtcc tcattaacgg gaggattttt ttctaaatag tctttcatat
    53761 atttacgcgc taggaaaaag ccacctactg cgccgattag caaagcaata atagcaataa
    53821 gtacgacaat acctgttgat accatataaa tctctccttt ctatttccga attgctctca
    53881 atgatttatt ttactaaagt ttattgaaaa aagaaaggcg tattctttag tttcatgcaa
    53941 aaaatagtgg tttttttaga gaagaacaaa atttctttca ttaaccagca agacacgcca
    54001 cgaaaaaaaa ttaggcatac cgaatctttt tctttaattt tttctttctg aggtgtagaa
    54061 tgtaatcgta gataaatagg aaaggtggtt ttttcttgaa taatcaatcc aacaaacaat
    54121 ttcataagca tcgacttatt gaacatacaa atggtttatc tttagctgaa gtaaatggaa
    54181 cagtggatgt accaaaggga aaaagttttt ttaaaacact tcttgcttac tctggaccag
    54241 gtgcattagt agcagttggt tatatggatc caggtaactg gtccacttcg atcactggtg
    54301 gacaaaattt ccaatattta ttgatgtcag ttattttgat gtctagtcta attgctatgc
    54361 ttttacagta tatggcagca aaattaggaa tcgtgaccca aatggatttg gcccaagcaa
    54421 tccgagcacg gacaagtaag acattgggtg tcattttatg gatcttgact gaattagcca
    54481 ttatggcaac agacatcgca gaagtcattg gtgcagcaat cgccctctac ttactttttg
    54541 atattccatt ggtaattgcc gtatttatca ctgtgttaga tgttttcgtc cttttattgc
    54601 tgacaaaagt cgggtttcgt aaaatcgaag ctttggttgt ctgcttgatt ttcgtcattt
    54661 tattcgtttt cgtctatcaa gttggcttgt ctaaccctga ttggcacagt ttatttgctg
    54721 gctttatccc taacagtcat acattctcag actcacctgt cataggtggt gaaactccgc
    54781 tgactggagc gttagggatt attggtgcaa cagtcatgcc gcataactta tatcttcatt
    54841 cagcagtttc tcaaactcgg aagatcgatc accaagacga agaagatgta gcacaagcgg
    54901 tacgcttttc tacttgggac tctaatatcc agttgtcaat ggctttttta gtcaatgcac
    54961 tgctattgat catgggtgtc gctgtattca aaaccggttc tgtccaagat ccttcctttt
    55021 ttggtctgta tgaagcttta tctgatccat cagctatgag caatggtatc ttgattgctg
    55081 tagcaaaatc tggtatcttg tctactttat ttgctgtagc acttcttgct tctggccaga
    55141 actctactat tactggtacg ttgaccggtc aagtcatcat ggaaggattc atccatatgc
    55201 gtatcccgat ttggctaaga agactagtca cacgattgct ttctgtgatt cctgtattga
    55261 tttgtgtttt gtttacaagt acaaaaggaa cgatcgaaga acataccgct ttgaataact
    55321 tgatgaatga atcccaagtt tttctggctt ttgcattgcc attctcgatg attcctttac
    55381 tgatgatgac caatagccaa gcggagatgg ggagccgatt caaaaattca tttgttgtaa
    55441 aacttcttgg ctggttctct gtgattagtc tgacttactt gaacttacga ggattaccag
    55501 gtcaaatcga aagtttcttc ggcgagcaag ccacctcagc tcaactagca ttggctgatc
    55561 aaatcgccta tgtaatcatt gttcttgtcc ttctcctgtt ggcatggacg attggtgatt
    55621 tatataaagg aaacaaaaaa tatgaacagc gtttgcaaga aacgatagaa gaaatcgaat
    55681 agaaactgat tcttttacac aaaggaggtt gtgacagaag tgggcagctt caagaaataa
    55741 gaaggaattt aagaaaattg cttttcaaat ttttgtgaaa tttcggctta tttccgaaga
    55801 agttacttct ggaacaccgt gtat
//
LOCUS       DBNIBO010000006        54283 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.006, whole genome shotgun sequence.
ACCESSION   DBNIBO010000006 DBNIBO010000000
VERSION     DBNIBO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 54283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.006"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="AC1Y77_000286"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="AC1Y77_000286"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIQ1573896.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="AC1Y77_000287"
     CDS             complement(937..1518)
                     /locus_tag="AC1Y77_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1573897.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="AC1Y77_000288"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="AC1Y77_000288"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIQ1573898.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="AC1Y77_000289"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="AC1Y77_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIQ1573899.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="AC1Y77_000290"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="AC1Y77_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIQ1573900.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="AC1Y77_000291"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="AC1Y77_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIQ1573901.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="AC1Y77_000292"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="AC1Y77_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIQ1573902.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="AC1Y77_000293"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="AC1Y77_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIQ1573903.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="AC1Y77_000294"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="AC1Y77_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIQ1573904.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="AC1Y77_000295"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="AC1Y77_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIQ1573905.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="AC1Y77_000296"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="AC1Y77_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIQ1573906.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="AC1Y77_000297"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="AC1Y77_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIQ1573907.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="AC1Y77_000298"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="AC1Y77_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIQ1573908.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="AC1Y77_000299"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="AC1Y77_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIQ1573909.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="AC1Y77_000300"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="AC1Y77_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIQ1573910.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="AC1Y77_000301"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="AC1Y77_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIQ1573911.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="AC1Y77_000302"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="AC1Y77_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIQ1573912.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="AC1Y77_000303"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="AC1Y77_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIQ1573913.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="AC1Y77_000304"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="AC1Y77_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIQ1573914.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="AC1Y77_000305"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="AC1Y77_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIQ1573915.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="AC1Y77_000306"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="AC1Y77_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIQ1573916.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="AC1Y77_000307"
     CDS             13972..14157
                     /locus_tag="AC1Y77_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIQ1573917.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="AC1Y77_000308"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="AC1Y77_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIQ1573918.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="AC1Y77_000309"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="AC1Y77_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIQ1573919.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="AC1Y77_000310"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="AC1Y77_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIQ1573920.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="AC1Y77_000311"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="AC1Y77_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIQ1573921.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="AC1Y77_000312"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="AC1Y77_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIQ1573922.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="AC1Y77_000313"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="AC1Y77_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIQ1573923.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="AC1Y77_000314"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="AC1Y77_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIQ1573924.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="AC1Y77_000315"
     CDS             18232..18879
                     /locus_tag="AC1Y77_000315"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIQ1573925.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="AC1Y77_000316"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="AC1Y77_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIQ1573926.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="AC1Y77_000317"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="AC1Y77_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIQ1573927.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="AC1Y77_000318"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="AC1Y77_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIQ1573928.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="AC1Y77_000319"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="AC1Y77_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIQ1573929.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="AC1Y77_000320"
     CDS             20317..21255
                     /locus_tag="AC1Y77_000320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIQ1573930.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="AC1Y77_000321"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="AC1Y77_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIQ1573931.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="AC1Y77_000322"
     CDS             21935..22774
                     /locus_tag="AC1Y77_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIQ1573932.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="AC1Y77_000323"
     CDS             22750..23619
                     /locus_tag="AC1Y77_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIQ1573933.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="AC1Y77_000324"
     CDS             23609..24406
                     /locus_tag="AC1Y77_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIQ1573934.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="AC1Y77_000325"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="AC1Y77_000325"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIQ1573935.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="AC1Y77_000326"
     CDS             complement(25688..28357)
                     /locus_tag="AC1Y77_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIQ1573936.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /gene="rpmB"
                     /locus_tag="AC1Y77_000327"
     CDS             complement(28595..28783)
                     /gene="rpmB"
                     /locus_tag="AC1Y77_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIQ1573937.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="AC1Y77_000328"
     CDS             29053..29415
                     /locus_tag="AC1Y77_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIQ1573938.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="AC1Y77_000329"
     CDS             29467..31149
                     /locus_tag="AC1Y77_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIQ1573939.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="AC1Y77_000330"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="AC1Y77_000330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIQ1573940.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="AC1Y77_000331"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="AC1Y77_000331"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIQ1573941.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="AC1Y77_000332"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="AC1Y77_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIQ1573942.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="AC1Y77_000333"
     CDS             34984..35988
                     /locus_tag="AC1Y77_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573943.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="AC1Y77_000334"
     CDS             35989..36933
                     /locus_tag="AC1Y77_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573944.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /gene="opp4B"
                     /locus_tag="AC1Y77_000335"
     CDS             36933..37895
                     /gene="opp4B"
                     /locus_tag="AC1Y77_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIQ1573945.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="AC1Y77_000336"
     CDS             37922..38836
                     /locus_tag="AC1Y77_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1573946.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="AC1Y77_000337"
     CDS             38862..40643
                     /locus_tag="AC1Y77_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1573947.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /gene="rnc"
                     /locus_tag="AC1Y77_000338"
     CDS             40991..41677
                     /gene="rnc"
                     /locus_tag="AC1Y77_000338"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIQ1573948.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="AC1Y77_000339"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="AC1Y77_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIQ1573949.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="AC1Y77_000340"
     CDS             45287..46096
                     /locus_tag="AC1Y77_000340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1573950.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="AC1Y77_000341"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="AC1Y77_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIQ1573951.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="AC1Y77_000342"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="AC1Y77_000342"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HIQ1573952.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="AC1Y77_000343"
     CDS             complement(49648..50100)
                     /locus_tag="AC1Y77_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HIQ1573953.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="AC1Y77_000344"
     CDS             50250..51200
                     /locus_tag="AC1Y77_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1573954.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="AC1Y77_000345"
     CDS             51193..52152
                     /locus_tag="AC1Y77_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIQ1573955.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="AC1Y77_000346"
     CDS             52149..52904
                     /locus_tag="AC1Y77_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573956.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="AC1Y77_000347"
     CDS             52927..53880
                     /locus_tag="AC1Y77_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1573957.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        17816 a         9837 c        11690 g        14940 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taagtccact agactagaaa atatgagaag cta
//
LOCUS       DBNIBO010000007        42900 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.007, whole genome shotgun sequence.
ACCESSION   DBNIBO010000007 DBNIBO010000000
VERSION     DBNIBO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.007"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(96..1247)
                     /locus_tag="AC1Y77_000348"
     CDS             complement(96..1247)
                     /locus_tag="AC1Y77_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIQ1573958.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1401..1931)
                     /locus_tag="AC1Y77_000349"
     CDS             complement(1401..1931)
                     /locus_tag="AC1Y77_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIQ1573959.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(2030..2380)
                     /locus_tag="AC1Y77_000350"
     CDS             complement(2030..2380)
                     /locus_tag="AC1Y77_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HIQ1573960.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2537..4066)
                     /gene="ahpF"
                     /locus_tag="AC1Y77_000351"
     CDS             complement(2537..4066)
                     /gene="ahpF"
                     /locus_tag="AC1Y77_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HIQ1573961.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(4076..4639)
                     /gene="ahpC"
                     /locus_tag="AC1Y77_000352"
     CDS             complement(4076..4639)
                     /gene="ahpC"
                     /locus_tag="AC1Y77_000352"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HIQ1573962.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4783..5445)
                     /locus_tag="AC1Y77_000353"
     CDS             complement(4783..5445)
                     /locus_tag="AC1Y77_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1573963.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5629..7275
                     /locus_tag="AC1Y77_000354"
     CDS             5629..7275
                     /locus_tag="AC1Y77_000354"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIQ1573964.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7266..8348
                     /gene="uxuA"
                     /locus_tag="AC1Y77_000355"
     CDS             7266..8348
                     /gene="uxuA"
                     /locus_tag="AC1Y77_000355"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIQ1573965.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(8456..10009)
                     /locus_tag="AC1Y77_000356"
                     /pseudo
     CDS             complement(8456..10009)
                     /locus_tag="AC1Y77_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            10157..11298
                     /locus_tag="AC1Y77_000357"
                     /pseudo
     CDS             10157..11298
                     /locus_tag="AC1Y77_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(11424..12503)
                     /locus_tag="AC1Y77_000358"
     CDS             complement(11424..12503)
                     /locus_tag="AC1Y77_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1573966.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(12612..13052)
                     /locus_tag="AC1Y77_000359"
     CDS             complement(12612..13052)
                     /locus_tag="AC1Y77_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1573967.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(13109..13519)
                     /locus_tag="AC1Y77_000360"
     CDS             complement(13109..13519)
                     /locus_tag="AC1Y77_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HIQ1573968.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13700..14134)
                     /locus_tag="AC1Y77_000361"
     CDS             complement(13700..14134)
                     /locus_tag="AC1Y77_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1573969.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(14234..15082)
                     /locus_tag="AC1Y77_000362"
     CDS             complement(14234..15082)
                     /locus_tag="AC1Y77_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1573970.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            15471..17237
                     /locus_tag="AC1Y77_000363"
     CDS             15471..17237
                     /locus_tag="AC1Y77_000363"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIQ1573971.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            17339..18598
                     /locus_tag="AC1Y77_000364"
     CDS             17339..18598
                     /locus_tag="AC1Y77_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIQ1573972.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            18745..20046
                     /locus_tag="AC1Y77_000365"
     CDS             18745..20046
                     /locus_tag="AC1Y77_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1573973.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            20049..20900
                     /locus_tag="AC1Y77_000366"
     CDS             20049..20900
                     /locus_tag="AC1Y77_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIQ1573974.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            20913..21728
                     /locus_tag="AC1Y77_000367"
     CDS             20913..21728
                     /locus_tag="AC1Y77_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HIQ1573975.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            21879..23045
                     /locus_tag="AC1Y77_000368"
     CDS             21879..23045
                     /locus_tag="AC1Y77_000368"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1573976.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            23275..23922
                     /locus_tag="AC1Y77_000369"
     CDS             23275..23922
                     /locus_tag="AC1Y77_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIQ1573977.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            24006..24950
                     /locus_tag="AC1Y77_000370"
     CDS             24006..24950
                     /locus_tag="AC1Y77_000370"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HIQ1573978.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            25085..26536
                     /locus_tag="AC1Y77_000371"
     CDS             25085..26536
                     /locus_tag="AC1Y77_000371"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIQ1573979.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            26557..27393
                     /locus_tag="AC1Y77_000372"
     CDS             26557..27393
                     /locus_tag="AC1Y77_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIQ1573980.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            27393..28052
                     /locus_tag="AC1Y77_000373"
     CDS             27393..28052
                     /locus_tag="AC1Y77_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1573981.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            28178..28867
                     /locus_tag="AC1Y77_000374"
     CDS             28178..28867
                     /locus_tag="AC1Y77_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573982.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            28873..29829
                     /locus_tag="AC1Y77_000375"
     CDS             28873..29829
                     /locus_tag="AC1Y77_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HIQ1573983.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            29873..30385
                     /locus_tag="AC1Y77_000376"
     CDS             29873..30385
                     /locus_tag="AC1Y77_000376"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1573984.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(30641..31189)
                     /locus_tag="AC1Y77_000377"
     CDS             complement(30641..31189)
                     /locus_tag="AC1Y77_000377"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIQ1573985.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(31278..32459)
                     /locus_tag="AC1Y77_000378"
     CDS             complement(31278..32459)
                     /locus_tag="AC1Y77_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIQ1573986.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(32695..34203)
                     /locus_tag="AC1Y77_000379"
     CDS             complement(32695..34203)
                     /locus_tag="AC1Y77_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIQ1573987.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            34362..34553
                     /locus_tag="AC1Y77_000380"
     CDS             34362..34553
                     /locus_tag="AC1Y77_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573988.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            34756..35673
                     /locus_tag="AC1Y77_000381"
     CDS             34756..35673
                     /locus_tag="AC1Y77_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIQ1573989.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            35666..36739
                     /locus_tag="AC1Y77_000382"
     CDS             35666..36739
                     /locus_tag="AC1Y77_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573990.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            36887..37405
                     /locus_tag="AC1Y77_000383"
     CDS             36887..37405
                     /locus_tag="AC1Y77_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573991.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            37386..37778
                     /locus_tag="AC1Y77_000384"
     CDS             37386..37778
                     /locus_tag="AC1Y77_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIQ1573992.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            37854..38789
                     /locus_tag="AC1Y77_000385"
     CDS             37854..38789
                     /locus_tag="AC1Y77_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIQ1573993.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            38922..39701
                     /locus_tag="AC1Y77_000386"
     CDS             38922..39701
                     /locus_tag="AC1Y77_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIQ1573994.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            39694..40011
                     /locus_tag="AC1Y77_000387"
     CDS             39694..40011
                     /locus_tag="AC1Y77_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIQ1573995.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            40255..41865
                     /locus_tag="AC1Y77_000388"
     CDS             40255..41865
                     /locus_tag="AC1Y77_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIQ1573996.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            42022..42870
                     /locus_tag="AC1Y77_000389"
     CDS             42022..42870
                     /locus_tag="AC1Y77_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1573997.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        14153 a         7746 c         8183 g        12818 t
ORIGIN      
        1 aagtacagta aaaggactgt ggcgttatcg tcacagtcct tttttcaaat aaacggtttt
       61 cacgatgtct ctttcacaac ttctttttga tttactcaca cttcataaac tttttttgta
      121 aaatatttga gaacaagtgg agaaataagt gtacttaata aaatcacgat aactaaaggt
      181 gaatagtact gtggttcgat caattgactt tgctgtccaa tttgtaaaat gatcaaagcc
      241 atttctccac gcgaaatcat accagcaccg accatcaatg aactatgttg agaaaaaccg
      301 gcaatctgag caccaaaata acctccaagc aatttggtca aaatcgctac aagcgtcaat
      361 atagaaataa agagtagttg ttcactaaag tcagaaaaat caacttctaa tcctacactg
      421 acaaaaaata ctggtataaa tactgcatat cctaaagcct ctacattgtg gtatacttct
      481 tctttaacct ttgtctgact gacagctatc ccagcaaaaa aagcaccaat gacagagcta
      541 agcccaacaa gatccgctac gtaagacatt cctaaacaaa taactagcga tacgatgata
      601 atagaagaat ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc aatccatttg
      661 actaataaga aaataaaaac aaaataaatt agctgttcaa tcaaaacaag cggtaaagaa
      721 gcattgctag aagcttcccc cgttagaaaa gataggctga aacttagaac taaaacgact
      781 aaaatgtcat ctaccacaga agcacccaaa attgttgatc cttcttttgt attcactacg
      841 tttagttctt ttaatacttc tactgaaata ctgacagaag ttgccgcaag aataatccca
      901 aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc ctgtcaacca gccaaaaaca
      961 accgggaaga tgatcccaag caaagcaacg aacataccag gtcggaaata ttttttcaaa
     1021 agagataaat cactttccag accagctaaa aacattaata agatcacacc aatctccgaa
     1081 aagtcatgga ctagaatatc aggtttgacc caattcaatc cagcttggcc tagcaaaaca
     1141 ccaacaagta attgtccaat cactgctggg atacctaatc tccgcgagat atgtgagcca
     1201 atcgttgttg aaatcaatat caggcataaa ataccaataa attccattct catcattcct
     1261 ttgtataaaa tatagcccga acttagcata acatacatta ttgaaaaaat aggaaaatat
     1321 actcacaaaa aaaccaacaa tccaaaagat ttttttggat tgttggtttt tatacggtct
     1381 attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat gaaattgata
     1441 atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag aagcatcaaa
     1501 cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat actactataa
     1561 acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa gctgttgatg
     1621 ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc aaaagcatag
     1681 acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact ccaagaacct
     1741 gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa aacaagatag
     1801 gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac aatcgcaatc
     1861 ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt ttgtttaatc
     1921 gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag tcattttacc
     1981 aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat caacttctga
     2041 ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct aaataacgaa
     2101 tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca acacctgtaa
     2161 tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg aaagtcactt
     2221 gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa caaatgattt
     2281 catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac ataggataaa
     2341 cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac ctttcttttt
     2401 ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg attattcaaa
     2461 aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct ctctgacttt
     2521 ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag
     2581 ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa tcaccagctg
     2641 cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca tttcttggat
     2701 tcatttcgat cgttctatct agccaatccg tattagggag taagccgact agaatgaata
     2761 ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag gagagagatt
     2821 ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca gtgacattcg
     2881 caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt aagaattcta
     2941 gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca ccagaattac
     3001 caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag tgtgggcaat
     3061 aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa cgccagtgcg
     3121 cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct aagccaatat
     3181 taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata atgtcgatag
     3241 ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct gtgtaagagg
     3301 taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa gaatcagcga
     3361 caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta gctgctggtc
     3421 caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct tcaatcaaag
     3481 ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta aattcttctc
     3541 catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct ttttggaaca
     3601 tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat atattcaatg
     3661 cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca aaatgcaatg
     3721 tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt gcccgtccac
     3781 taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg ataccagcaa
     3841 aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg cgcatcgaat
     3901 ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta aattcagcta
     3961 gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga gattcaatca
     4021 gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc accacctaaa
     4081 tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc cattttgccg
     4141 gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta tcaatcagtg
     4201 cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg attccgtctg
     4261 gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa acatcaaaaa
     4321 tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta ccgatagcat
     4381 ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt
     4441 caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat tcagtaggac
     4501 aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc ataagatctt
     4561 tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat tcttcgattt
     4621 ttttcccaat caatgacata gtaaaacctc caataataat tatttattta taattattgt
     4681 aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga ttactcaaaa
     4741 acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct ggaaatactc
     4801 gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt
     4861 ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat cataatgttg
     4921 ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc ggaaacgttc
     4981 taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg caatttcgaa
     5041 acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac
     5101 tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt
     5161 ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc gagcattttc
     5221 tgcagaaaca agattaattt tggaaacata cgtaccactt tgaggtatcg tatacacgag
     5281 ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga tagcaagatg
     5341 ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat agataatttg
     5401 tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta ttcctccaaa
     5461 atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg tatgtcacat
     5521 taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt ttattgcata
     5581 aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat gattgattta
     5641 aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat acctcatttt
     5701 gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca tttcggtggc
     5761 ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa
     5821 ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga aagggcttac
     5881 caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa
     5941 cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc tgaacacttt
     6001 gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt tgctattact
     6061 gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat tgcagtcgat
     6121 ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgt gctactttat
     6181 aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa tttttcacaa
     6241 aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca aaaggaaggt
     6301 tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc gtttccatgg
     6361 acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa
     6421 gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat tgctccattt
     6481 gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa tggacgtccg
     6541 aaattcgaaa aagctggggt attcttgact gatcgtgcaa cggtagataa agcagatacg
     6601 atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat gggttgttta
     6661 ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct gctagtttct
     6721 gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat tgatccaaag
     6781 atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc taatatgcct
     6841 cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga gacgattaaa
     6901 aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc attgactctc
     6961 gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt catcccgagt
     7021 ccagacccac tattgacaga ccttcagcat aagctttccg atattcaact gggtacgcaa
     7081 gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga aatttttggt
     7141 gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat caagatggta
     7201 gcaggaccaa atgccgtacg aaaaacatta caggattact tacaagaaca tggaggaaat
     7261 ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt accttagatg
     7321 aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat ataccagtag
     7381 gagaggtatg gcctagggag aggatcaaag aactaaaaga agaaattgag tctcgtgggt
     7441 taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt ggattacctt
     7501 cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca gaatatggaa
     7561 tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct gaccttgact
     7621 accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt ccagatgatc
     7681 caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttggattg ccgggatggg
     7741 aacctgagcg cttagctcct atcaaatcct tgcttgaagc ctatgcatca gtagacgaag
     7801 aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca tgtgaagagg
     7861 taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt ggattagctc
     7921 gaatcgtaaa gaatcgagaa gatttagatt ggttgtgtcg catagtagac agcccttata
     7981 acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat gtatatggga
     8041 ttttagcaga attttgccaa agagatcgaa tcccatttgc acatgttcga aatattaaat
     8101 ttttagctaa tcgagatttc tatgaggcac cacataaatc cgaatacggt tcattagaca
     8161 tgtaccgcat actaaaaaca atgtatgaca acggatttaa cggatacatc cgtcctgatc
     8221 atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat gatcgagcat
     8281 taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac aaaagtaaag
     8341 ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt caaattcttg
     8401 atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat ttggtctagg
     8461 aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt aattcaaaga
     8521 tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg attcctttta
     8581 aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg ctgataacga
     8641 tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa aaaatttgtt
     8701 ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc acaccaatca
     8761 caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca accatccatt
     8821 gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct ctgaaatcca
     8881 tataaaattg ttcaatggcg gtttggactt ctttcaatac gctatgttct tgataaagca
     8941 tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta taaagtttat
     9001 ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac aaatcaaata
     9061 cagtacaaag ggactccacc atttctcttg aataaagaac atgaggaacg tttgcttttt
     9121 ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg atagaaagtc
     9181 gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa
     9241 tatctacaga gagatcaata aaaaagtcaa tatattcata ctctgtgaat tctttttgta
     9301 cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga aaatataatt
     9361 tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca
     9421 taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt gaaaagatgt
     9481 tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa aaagactttc
     9541 tgccattatt agaaaaggag atagacaaac cgtactatgc taaaaatgca ttaatctttt
     9601 tcattttcgt atacaaataa gaggcactat agtttgtttg ctgactgaaa ctgataatcg
     9661 aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat tcgttgcagt
     9721 atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa ttcaacttta
     9781 aaaaaggttt ttgcttatca atatgcagtt ctgttagatt gagacgctca aagtcttgtt
     9841 ctaattcttt gatgtatagt aaaaaatttg attcagacat atggaaatga tctgctaaat
     9901 attgtgtagt aataaagaaa cgatgttcag ataggaaaag aaaaatatct aattttgtat
     9961 tgcttttttt cttcaagata aacgttttaa tcgcttcttg agtttgcatg aaagaggctc
    10021 ctttttactt aaattgttaa aacttgaaaa tattactgtt tttttcataa atttagacaa
    10081 aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata caaaaaaatc
    10141 aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg cagccttaag
    10201 cttatcgctt actataaatc agacagtcca cacagaggag acagaaacag ccaaatggac
    10261 agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt catccttcaa
    10321 ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa aaattgacta
    10381 ttcgggagac ttagcaccta tactacaatc agctgttaca tttgagaatg ttgatcccac
    10441 tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag cgatatttac
    10501 cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg tcaaggtatc
    10561 agatggacta ccgaacgatc atcatttaac gccgtatagt cagcggcaga caattgagca
    10621 agattcagcc atcgactcag gaggagatct tgttgaagaa ccaacagata aaccagaaaa
    10681 tgaaaataag ccagaagtac cgcctacaga aaatccagac ggagaacaga aaccggaaat
    10741 agaacctgga gaagaacccg atacagaaac tcaaccagag ccagacaata aatcaaaacc
    10801 tgaaataacc cctggagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa
    10861 ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc
    10921 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
    10981 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
    11041 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
    11101 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
    11161 tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata
    11221 ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa
    11281 caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga
    11341 aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata
    11401 ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg
    11461 atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag
    11521 ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc
    11581 agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca
    11641 atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg
    11701 aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa
    11761 atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg
    11821 agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg
    11881 acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa
    11941 aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg
    12001 aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc
    12061 gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat
    12121 tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aatcaataac ggactggatc
    12181 atatcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg
    12241 taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc
    12301 tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt
    12361 gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg
    12421 cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa
    12481 caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa gagaaagagc
    12541 aaaaagaaaa accgctaaat gacgttatct tatgagttgg ttttcctcat aaaatcgttc
    12601 atctagcagt ttcagccctt tttctggtct tgatgaaaat tatctagcat ttttcgggtc
    12661 agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa gataagactt gatcttttgt
    12721 ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag gagtcagcgt aacatttacg
    12781 attctttcat cacccgtttc acgctgacga ttaatataat ttttttctgc taactttttt
    12841 agtaagggag tcagcgtacc agaatccaga cacaaatcat ttcctagctc tttcactgaa
    12901 gtctttttct tttcccataa aactaacatc acaagatact gggtataggt aagattcagc
    12961 ggcttgagga gggaagtgta gtgtctaatg atttccttag aggcggcata taaaggaaaa
    13021 catacttgat taactaaata tagatcttcc atttttccac ctccgctgtt ttactatagt
    13081 gtatcatagc ggatttacac aaaaaagtct attcactaac tgctttcaga gtaacttcta
    13141 tatttccagc tgtagctttt gaatacggac acactttatg cgctgcctct aataattctt
    13201 gcgtttcttc cagagaaaga ccttcgatag atccttcaat ctctacacct aagacaaagc
    13261 tcgtttcact ttctgaataa agggaaactt gtgcactgac agtagaagcc ccagagatat
    13321 ttttttgctg tttgatcaat tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa
    13381 acaattgttc agggttggtt gcattttcga cgcgtttacc aggagctgca atattcaatt
    13441 cgaatgtacg atcgggatta tgaacttctc cagagcgtcc gcctgtgttg atcattttcg
    13501 tagaatacat ttttttcata aaattaccac ctttaattat attttataca attgaatttt
    13561 acacaatata attgtggaaa gcaaagaaat ggattggcat aagataaata agagaaagca
    13621 catgaaacag acgcgctttc tcgaatagca aacatataca aaaaaatatc agagacaaaa
    13681 actaatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaac
    13741 ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg
    13801 agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc
    13861 gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat
    13921 ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga
    13981 atctcgtgcc atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct
    14041 caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat
    14101 ttaatacaaa atctatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga
    14161 acagttacta gattctttta ttataccagt taaagaaaac gcattcaaaa aaatagcgga
    14221 tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta
    14281 cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg
    14341 aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc
    14401 cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt
    14461 cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga
    14521 tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta
    14581 ttccaaaaac aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg
    14641 aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag
    14701 aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga
    14761 cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa
    14821 aaaacaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta
    14881 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaat cctaaaacag
    14941 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
    15001 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
    15061 taaattgtct cgtttctttc atttatccac ttccttaatt cattaataat actcttatct
    15121 tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct
    15181 aaattattat gtttcattct tctagaatat acttctacgt taaaaactgt taaaaaactt
    15241 ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac
    15301 aggaagtaga ttaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat
    15361 aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgttt
    15421 ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag
    15481 ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc
    15541 gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgctt tgtggagatc
    15601 catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa
    15661 gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagg cgcttacagt
    15721 atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt
    15781 ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc
    15841 atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt
    15901 ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg
    15961 gaagtaaaga tccagaccga gaagacttct tcgggggaga tttgcaaggt gtgagggatc
    16021 atttggatta cttgacggac cttggcgtca atggcatcta tttttgtccc atatttaaag
    16081 ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg
    16141 ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt
    16201 tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa
    16261 acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt
    16321 atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta
    16381 ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata
    16441 tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg
    16501 aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca
    16561 tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc
    16621 atgcagtgat gaattatgca ttcacagatt cgattaaaga ttattttgca aaaaagaaaa
    16681 tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg
    16741 tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt
    16801 gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt
    16861 ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc
    16921 gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag
    16981 aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc
    17041 taatagtata tgatcgtgaa gataagctct atttcacacg ggaactagag ggacagatca
    17101 tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga
    17161 ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat
    17221 ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga
    17281 tttgtgccca ttataataag ggtgtaacag cacgttgaat acacaggagg aaatgaagat
    17341 gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg
    17401 attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac
    17461 gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt
    17521 cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga
    17581 atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga
    17641 ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc
    17701 agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc
    17761 tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc
    17821 aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc
    17881 aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat
    17941 tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt
    18001 gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg
    18061 gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc
    18121 caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa
    18181 tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt
    18241 tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa
    18301 atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga
    18361 aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac
    18421 agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga
    18481 agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa
    18541 agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt
    18601 aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa
    18661 gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt
    18721 agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa
    18781 aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt
    18841 aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga
    18901 tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta
    18961 tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac
    19021 ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc
    19081 aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg
    19141 acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct agttacattg
    19201 ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt aattgattgg
    19261 gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt
    19321 gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt
    19381 ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt
    19441 atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat
    19501 atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt
    19561 ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca
    19621 attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc
    19681 ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg
    19741 cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt
    19801 acacagtata cggggaattt caataatttc tccatgatct atctgtttaa caatggtggt
    19861 cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg gatctataaa
    19921 cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc
    19981 gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg
    20041 gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt
    20101 ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag
    20161 caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact
    20221 ggactttagg aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa
    20281 atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg
    20341 gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag
    20401 tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat
    20461 tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa
    20521 tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg
    20581 ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca
    20641 aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt
    20701 tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat
    20761 ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg
    20821 cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat
    20881 ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt
    20941 ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat
    21001 ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg
    21061 ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg
    21121 aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc
    21181 aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac
    21241 aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg
    21301 ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg
    21361 tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc
    21421 caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc
    21481 tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca
    21541 ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg
    21601 cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa
    21661 cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg
    21721 tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag
    21781 tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt
    21841 attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca
    21901 atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa
    21961 cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa
    22021 tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc tatatggatc
    22081 atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa
    22141 acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga
    22201 aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac
    22261 tttggctaga tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt
    22321 tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag
    22381 gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag
    22441 agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca
    22501 aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg
    22561 aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt
    22621 ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag
    22681 ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat
    22741 atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa
    22801 acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac
    22861 attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct
    22921 tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata
    22981 tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg
    23041 cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg
    23101 ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt
    23161 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt
    23221 ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa
    23281 aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat
    23341 accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta
    23401 tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa
    23461 gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat
    23521 aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc
    23581 tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat
    23641 gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag
    23701 aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc
    23761 gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa agatttaggt
    23821 actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat
    23881 agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca
    23941 agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga
    24001 tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg
    24061 catctggtgt ccactgcatg actacccagg aattagatga actggctcat tcagaagcag
    24121 gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct
    24181 attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt
    24241 attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat
    24301 ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa
    24361 aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac
    24421 cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct
    24481 tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga
    24541 tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca
    24601 atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg
    24661 gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata
    24721 cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg
    24781 catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtacagag cttcataaag
    24841 aagggccgga aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag
    24901 gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg
    24961 aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc
    25021 aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga
    25081 taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca
    25141 agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt
    25201 tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa
    25261 agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg
    25321 gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta
    25381 tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt
    25441 tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct
    25501 tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt
    25561 tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg
    25621 attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg
    25681 atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt
    25741 attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg
    25801 gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca
    25861 ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa
    25921 agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc
    25981 ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat
    26041 ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg
    26101 tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat
    26161 tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac
    26221 cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat
    26281 cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt
    26341 gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc
    26401 tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag
    26461 agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc
    26521 aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct
    26581 ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa
    26641 gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca
    26701 ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg
    26761 gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat
    26821 ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc
    26881 acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt
    26941 atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa
    27001 aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa
    27061 ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga
    27121 acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat
    27181 atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg
    27241 aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa
    27301 gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg
    27361 aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcccctaatt gatttttccc
    27421 tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct
    27481 tcgtgtcgtt tcttttagga aatttggtag ggttgctttt gacagtttta ggtcttttgg
    27541 attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg
    27601 cactagtcct gctgtttctg ttatatttcg ggttacctta tcaattaagt gccttaacgg
    27661 cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat
    27721 cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct
    27781 ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca
    27841 gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag
    27901 aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata
    27961 ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt
    28021 tagaaaaacg ttctatccat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg
    28081 caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt
    28141 aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga
    28201 aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt
    28261 cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg
    28321 aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc
    28381 caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga
    28441 agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga
    28501 agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct
    28561 tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag
    28621 aaaagcaaag caaatggagc gaggcgtaaa atattttcca aaagatcaat tgtacaaaga
    28681 attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt
    28741 tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca
    28801 gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt
    28861 ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct attagcatta
    28921 aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta
    28981 cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatccaatc
    29041 gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt
    29101 tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc
    29161 agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa
    29221 gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact
    29281 agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg
    29341 agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt
    29401 tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat
    29461 ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac
    29521 ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca
    29581 ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa
    29641 ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct
    29701 ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg
    29761 ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg
    29821 atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat
    29881 tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga
    29941 tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac
    30001 gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc
    30061 tggaacagct gcattagtct caggcgtgga tcccgtttac acaaaaatag atggtagttg
    30121 gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg
    30181 aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa
    30241 tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta
    30301 ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa
    30361 agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata aagacattaa
    30421 cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc
    30481 aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt
    30541 ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc
    30601 acgatctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt
    30661 ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga
    30721 tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa
    30781 tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg
    30841 taataaagat aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg
    30901 ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg
    30961 aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct
    31021 gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg
    31081 gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg
    31141 aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc
    31201 ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa
    31261 ctttgctttt ttgtatttta taccagcaag ccagtcacaa tagcacttaa aaagctgact
    31321 aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca
    31381 tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg
    31441 aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat
    31501 tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc
    31561 actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg
    31621 atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa
    31681 gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg
    31741 tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct
    31801 tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag
    31861 ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg
    31921 atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat
    31981 aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa
    32041 gctactccat tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca
    32101 acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa
    32161 ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca
    32221 ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt
    32281 gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca
    32341 agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg
    32401 atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa
    32461 taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc
    32521 tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata
    32581 aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg
    32641 aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg
    32701 gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt
    32761 caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag
    32821 ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac tgattgataa
    32881 agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt
    32941 caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg
    33001 ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt
    33061 acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt
    33121 tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg
    33181 gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc
    33241 gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg
    33301 tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc
    33361 catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt
    33421 gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc
    33481 aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc
    33541 aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta
    33601 accagctgca tgtccttttt ggctttcatg atacagttgg ttcaagcaag tagcgagctt
    33661 tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc
    33721 attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat
    33781 ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt
    33841 ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc cataaccacg
    33901 taattcatcg attaaccgca agatatccat atcataagta atcccataat caccacggat
    33961 cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa
    34021 tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg
    34081 cttgtcgtca actaacttcc caccaaattc taaatataat ttgtcatagt ggttcactcg
    34141 ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt
    34201 cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact
    34261 ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag
    34321 ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa
    34381 agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg
    34441 aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc
    34501 gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa
    34561 gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa
    34621 aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata
    34681 acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa
    34741 gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta
    34801 gaggagacga acgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa
    34861 gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct
    34921 gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa
    34981 ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat
    35041 gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg
    35101 agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt
    35161 ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg
    35221 gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag
    35281 acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg
    35341 aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag
    35401 aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct
    35461 gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt
    35521 gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga
    35581 ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat
    35641 caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa
    35701 tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa
    35761 tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac atgaaaaaga
    35821 aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga
    35881 aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga
    35941 aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt
    36001 tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat
    36061 cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat
    36121 tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa
    36181 ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca
    36241 acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa
    36301 tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc
    36361 tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac
    36421 acttggtttt accgttaatc agacattact agacaatacg aagccacttc gtttgaatct
    36481 tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa
    36541 aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa
    36601 aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc
    36661 tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt
    36721 aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg
    36781 ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact
    36841 aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa
    36901 aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt
    36961 tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt
    37021 aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt
    37081 tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat
    37141 cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct
    37201 ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc
    37261 tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa
    37321 tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg
    37381 aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga
    37441 caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat
    37501 cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgg
    37561 tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag
    37621 ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt
    37681 ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg
    37741 atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag
    37801 ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa
    37861 attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg
    37921 ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca
    37981 tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca
    38041 ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta
    38101 caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg
    38161 aaaagttgac gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt
    38221 tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat
    38281 tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac
    38341 tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa
    38401 caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg
    38461 gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg
    38521 gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga
    38581 tcagtcggct gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag
    38641 caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg
    38701 aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata
    38761 aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga
    38821 gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat
    38881 gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg
    38941 cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag
    39001 gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt
    39061 tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc
    39121 aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat
    39181 taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt
    39241 ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt
    39301 cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta
    39361 gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt
    39421 ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga
    39481 tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata
    39541 ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg
    39601 cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc
    39661 tcttcaactt cccggaacag gaggccaatc accatgacta aactatattt gctactttta
    39721 gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact
    39781 catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt
    39841 gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat
    39901 atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg
    39961 ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta
    40021 ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt
    40081 tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct
    40141 tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt
    40201 ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat
    40261 aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta
    40321 ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt
    40381 gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca
    40441 gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac
    40501 gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc
    40561 tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac
    40621 gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt
    40681 cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct
    40741 ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc
    40801 gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc
    40861 ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa
    40921 cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc
    40981 tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa
    41041 atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat
    41101 cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc
    41161 ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag
    41221 aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg
    41281 attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt
    41341 tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt
    41401 ttctctttat tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg
    41461 atccgattaa ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga
    41521 ttagttggcg gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt
    41581 cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat
    41641 tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc
    41701 tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg
    41761 gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt
    41821 cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt
    41881 tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc
    41941 aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt
    42001 tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg
    42061 ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat
    42121 gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa
    42181 aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat
    42241 gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt
    42301 tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt
    42361 ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac
    42421 ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat
    42481 aatacctatg cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg
    42541 acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct
    42601 gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct
    42661 gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata
    42721 catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca
    42781 aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa
    42841 gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat
//
LOCUS       DBNIBO010000008        36966 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.008, whole genome shotgun sequence.
ACCESSION   DBNIBO010000008 DBNIBO010000000
VERSION     DBNIBO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.008"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(129..557)
                     /locus_tag="AC1Y77_000390"
     CDS             complement(129..557)
                     /locus_tag="AC1Y77_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1573998.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(585..1223)
                     /locus_tag="AC1Y77_000391"
     CDS             complement(585..1223)
                     /locus_tag="AC1Y77_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1573999.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1227..3263)
                     /locus_tag="AC1Y77_000392"
     CDS             complement(1227..3263)
                     /locus_tag="AC1Y77_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HIQ1574000.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3389..4054
                     /locus_tag="AC1Y77_000393"
     CDS             3389..4054
                     /locus_tag="AC1Y77_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574001.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5989..9138)
                     /locus_tag="AC1Y77_000394"
     CDS             complement(5989..9138)
                     /locus_tag="AC1Y77_000394"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIQ1574002.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9151..10350)
                     /locus_tag="AC1Y77_000395"
     CDS             complement(9151..10350)
                     /locus_tag="AC1Y77_000395"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIQ1574003.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10340..11935)
                     /locus_tag="AC1Y77_000396"
     CDS             complement(10340..11935)
                     /locus_tag="AC1Y77_000396"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIQ1574004.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12444..13226)
                     /locus_tag="AC1Y77_000397"
                     /pseudo
     CDS             complement(12444..13226)
                     /locus_tag="AC1Y77_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13291..14127)
                     /locus_tag="AC1Y77_000398"
     CDS             complement(13291..14127)
                     /locus_tag="AC1Y77_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1574005.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14145..15554)
                     /locus_tag="AC1Y77_000399"
     CDS             complement(14145..15554)
                     /locus_tag="AC1Y77_000399"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1574006.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15568..17391)
                     /locus_tag="AC1Y77_000400"
     CDS             complement(15568..17391)
                     /locus_tag="AC1Y77_000400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1574007.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18234..18560)
                     /locus_tag="AC1Y77_000401"
     CDS             complement(18234..18560)
                     /locus_tag="AC1Y77_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574008.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18606..18968)
                     /locus_tag="AC1Y77_000402"
     CDS             complement(18606..18968)
                     /locus_tag="AC1Y77_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIQ1574009.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(19005..19220)
                     /locus_tag="AC1Y77_000403"
     CDS             complement(19005..19220)
                     /locus_tag="AC1Y77_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1574010.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19347..19682
                     /locus_tag="AC1Y77_000404"
     CDS             19347..19682
                     /locus_tag="AC1Y77_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574011.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19758..21755)
                     /locus_tag="AC1Y77_000405"
     CDS             complement(19758..21755)
                     /locus_tag="AC1Y77_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574012.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21853..22128)
                     /locus_tag="AC1Y77_000406"
     CDS             complement(21853..22128)
                     /locus_tag="AC1Y77_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574013.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22360..23094
                     /locus_tag="AC1Y77_000407"
     CDS             22360..23094
                     /locus_tag="AC1Y77_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1574014.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23133..24275
                     /locus_tag="AC1Y77_000408"
     CDS             23133..24275
                     /locus_tag="AC1Y77_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1574015.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24370..25761)
                     /gene="sufB"
                     /locus_tag="AC1Y77_000409"
     CDS             complement(24370..25761)
                     /gene="sufB"
                     /locus_tag="AC1Y77_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIQ1574016.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25766..26236)
                     /gene="sufU"
                     /locus_tag="AC1Y77_000410"
     CDS             complement(25766..26236)
                     /gene="sufU"
                     /locus_tag="AC1Y77_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIQ1574017.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26223..27458)
                     /locus_tag="AC1Y77_000411"
     CDS             complement(26223..27458)
                     /locus_tag="AC1Y77_000411"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIQ1574018.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27455..28741)
                     /gene="sufD"
                     /locus_tag="AC1Y77_000412"
     CDS             complement(27455..28741)
                     /gene="sufD"
                     /locus_tag="AC1Y77_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIQ1574019.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28754..29527)
                     /gene="sufC"
                     /locus_tag="AC1Y77_000413"
     CDS             complement(28754..29527)
                     /gene="sufC"
                     /locus_tag="AC1Y77_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIQ1574020.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29724..30572)
                     /locus_tag="AC1Y77_000414"
     CDS             complement(29724..30572)
                     /locus_tag="AC1Y77_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIQ1574021.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30596..31282)
                     /locus_tag="AC1Y77_000415"
     CDS             complement(30596..31282)
                     /locus_tag="AC1Y77_000415"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIQ1574022.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31275..32309)
                     /locus_tag="AC1Y77_000416"
     CDS             complement(31275..32309)
                     /locus_tag="AC1Y77_000416"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574023.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(32372..32628)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32704..33042)
                     /locus_tag="AC1Y77_000417"
     CDS             complement(32704..33042)
                     /locus_tag="AC1Y77_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIQ1574024.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33044..33400)
                     /locus_tag="AC1Y77_000418"
     CDS             complement(33044..33400)
                     /locus_tag="AC1Y77_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIQ1574025.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33406..34593)
                     /locus_tag="AC1Y77_000419"
     CDS             complement(33406..34593)
                     /locus_tag="AC1Y77_000419"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIQ1574026.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(34784..35629)
                     /locus_tag="AC1Y77_000420"
     CDS             complement(34784..35629)
                     /locus_tag="AC1Y77_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1574027.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35863..36828
                     /locus_tag="AC1Y77_000421"
     CDS             35863..36828
                     /locus_tag="AC1Y77_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HIQ1574028.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10958 a         7581 c         6124 g        12303 t
ORIGIN      
        1 atttttttgt tgtttttatt atgatatatt tttatacaac taaaaatgaa tatattaaaa
       61 ctaccaaaaa taacttttaa cgctgtttca ttatatgttt tttctatttt atataaactt
      121 ttggatactt aataccaacc ggttggattg taccaacatg agaatgtgtc agttctattt
      181 cctgtcgcac ttgcataggc ccatactcct tttgttgcat ttgctgatcg aatagagttt
      241 ccaactttcg cttttgcacc gtgacgatta tagacacgac tatagtggtt agaatgacct
      301 gtttttctcg tacaagttgt atcactataa taaacccatc ttccagagct atgattaatt
      361 gctgttctag aaatttcccg gtctactggt aaatttgact taataatatt acctttagca
      421 tcaaaatgaa tctctcctgg ctttaaagat ttgtaataga tttcaccgtg aactgaattt
      481 gcttccactt cagatgacat agcgtttcct ccaacaaaac ctatccctag tgcacaaata
      541 ataactgctt ttttcataaa acgacatcct ttcctttttg atttttaaag catgatcttc
      601 ttgtttccta ttttggtcag atatggatca tgcgtgacta cgataactaa cgtaccttgg
      661 gaagcttgtt gcgataagat ttcaactact ttatccttat ttcttttatc caatgaccca
      721 gtcggttcat cggctaaaat gattttgcta ttcttcaata agactctcgc acaggcgatt
      781 ctttgcttct caccaccaga taatgtgtag ataggcgtac ttagcttctt gtttatattt
      841 acttttttca atgcctcata ctttgctgtt cgcttatctg aaacatttaa attcgaaaac
      901 ttcaatccaa tatctaaatt aaaatcgatt gtttcatatt caatcaaccc aaaatcttga
      961 aaaagaaaag atatggtatt tctttttatc aagcgcttct ctctcccagt taattgtttc
     1021 gtctcttgat cgaacaaata gaattgtcct gaactgaact gatcattcaa tcctaatata
     1081 ttcaataacg tacttttacc tgctccactt tctccaacaa cagtcacaag atccccagca
     1141 tccgcagtga aacgatccaa atgaagaatc gttttgtttc gaatgatttt tttcgcacct
     1201 tttatcacta tcaacggctc caattgttaa cctccattca tcagcaataa ctgacttttc
     1261 ttttctgtag acaaaataaa aacaactgaa agaataaaat caatgaagct aaagaaaaaa
     1321 atattaaaaa gatatttagg ctctctttct ataagaaaaa ggaagattac acttgtcgtt
     1381 ccccatttga tgaggttcat catgaagaaa tctttatacg tttcaaagag cgaataacca
     1441 cttgaacgaa agatagcgat tttccgacaa ttgtagacaa aatatatgca taacatgaca
     1501 tagctcaaca agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa gtaatgaaat
     1561 gatctgctta acactttgac agctatctct tcagtctgag tatacggaaa tgataactgc
     1621 aagttatcac gtaactgact ttcttcaatc agctcaaata acctttcttt cgacaaagag
     1681 cctatcttga tctttaacgg tggatagatt tctccgctga gaatatttct ttccaaatac
     1741 atactgtttt tcaatgtcag tatttcaaca accggataat gttcgatcca ggggatagaa
     1801 ggaataaatg tataaatggg ttgattgttt tttataaata gaaatttcgg ttctttttca
     1861 ctgatttctt gataatattc tgttatttgc gccaggtcat tttttaaatc tgtatgtcta
     1921 tatcgttcag ggatcaatat aacagggtgc gtttcttttt cggttatttg gatcaacctt
     1981 ccagagacat caatcaaagg aaatttctta agataattca gatttatttt tatccctctg
     2041 ccaaactgtt cattttcttc attcaagtaa tctgatacat tgactaatat acttccgttt
     2101 ttattcaaag tttcatataa ttcctgatct gctgctgatg caaaacgatt ggaataaata
     2161 aaatccacgt ggtttttccc gatggttact ggataaaata catgatagtc ctcaccagcg
     2221 atttcttttg gtggttcatg ggaaacagct aactgtgaag aagaaaaaac gatagttgct
     2281 aattcgacat tggcaacggt caaaagaaga gaaaacaaca atccaaatgt aaaaagaatg
     2341 tattggatat agttgaacgc ttctcttaac gaagcgattt tttcaactaa taaaagagaa
     2401 atcaaagaga taccatatat tccgctgaac aagaaaacgt acatctggaa caaataaaga
     2461 aattgctcct tataatacat aaaaataaaa ccaccaatga ttcctgcaat tatgaatgta
     2521 ataatgattt tctttttccc caataaacac cacaatactt gatgtaatga aaagtcatta
     2581 ttcaacaata aactgatttc tttattcatc aataagaaaa aggatgataa caaaaggaat
     2641 aaaagaatca atccaatagt taaatacact cgataattat atacgtctgt atttgaaata
     2701 accgaatacg aatagctatt ttcaaagtct tcaaaaatat tagtggcata atcagtgtga
     2761 aatgcttgat tgaatcgatc agtgaatagt gaaatgaccc gttcataggc ttctttttct
     2821 ctcgtcatca agaaaaattc accttcaaaa tcttttattt tgatcgattc ttcaatggac
     2881 gttacgcgaa aggaggtatt agaatagggt accttctcgt gagaagattt gacatcccct
     2941 tgataataca atgtcatttc aagctttggc tgtatcttga agttccaaga tgtatctact
     3001 tcattggatt catttactgt ttttttcata taagaaagct cagtttctga tgagacattt
     3061 tcaaatacca tgattagctg ctcatattct tcttgcccaa taaaatgttg tggcaatcga
     3121 atcggagtat aagtatctgt ttgatggaaa aattcatttg tccgatagag tacctgttta
     3181 tttatactaa ccaaggcaaa accactcatg ataccggtca ctatgattgt gagtaaaact
     3241 gtgaactttt ttttgattat caccactctc ccttacttaa atgattcttt gataaaatca
     3301 taccgttttt catcaaaaaa acgtatctat caagtgtata ggtcatgtag aataaatcta
     3361 tatactgtag aatcgaaagg agaagaagat gaacaccgga gaaaaaatac gaacttaccg
     3421 cagaaattgt aagcttacgc aaaaagaatt aggcgaaaaa attggtgttt ccgataaaac
     3481 gatttctagt tgggaaaatt ctcgtacaat gccagacttt gaaatgctgt cacttttaca
     3541 tcatacttta ggtctgccga ttgattttcc ttctattgaa tcagctaccg aatcaataga
     3601 accaacattg ccagatgaac ctcttgttaa caagaaatct tattcatcaa aacaaccctt
     3661 ctcactcaaa aagacactat ctttctttct gctcgtcttt tgtttggtct atctttttct
     3721 tatcaattat caatacaaaa atgaaacgat cgatcgattc aatccttttt tgaaaccaca
     3781 aatcggctat accacactcc ctgtaggata cgtggacaat tccatcgaac gttgggtatg
     3841 taatgatatc tttggccatg gtacatggct gtcttttcac gctggagagg tagaaaaaga
     3901 gaaaaaccat gctgtaatca aacacaaagg aaaacacgtc atgaacgttc gcctgctcac
     3961 ttgggaagcc attccctcag attaccaaga aatcctcagc caagactatg acgacgcctt
     4021 aaaaagaagc cctcaggagc cagataggca ttaagtagga gatgaacgta gagatagaaa
     4081 aaatcaatgc acatgatctt atccaaaaaa caggggtaca aattaattga aaaagccttg
     4141 agatgaaagt tcctaaataa aaaagttcag aatgataggt atttctggct tatagtataa
     4201 tatttacgcc acagcctccc aatgattctt tttggaatca taatcaagga tggacattca
     4261 caaaaatatt ttgctcattc gatccgaata gattaacgac caatctagta agtaatatag
     4321 attggtacgt agtcaagctg cacataattt agatcaattt tagttctttt ttatatttct
     4381 atgatctgct catttacttt tatatgatgg cttacagtaa cataaataaa tatgataatc
     4441 gctggtagtt tatccaagag tactcaataa aatacaaaat ggattgttca agcgcctatc
     4501 ataatgataa aatgaaacaa ataaacgata caaggaggaa gagactttga gtaatggcag
     4561 aacaattttt tcattgtaaa aattgttcag ttgttaatct atgggatttc ggtagcatgg
     4621 gcattttttt atagaggaat cacaccaacc tggccgtttt atctcttgtt agtgggaata
     4681 ttagtaggaa attttgcccc tcttcactat cgtacgaact tttttccaac tgattctggc
     4741 ctttttctta aaacaaagac gcgaaaatta gaaaacatta ttgaaatgac aattacctgt
     4801 ataatcattt tcttagttat attcctaaac gtcttctaat aagaatctct taaaattagc
     4861 ttgttacagt tagttaccta gtttatataa cacagttatc ttatcttcct tatcaacatc
     4921 ttttgccaac tctttgtctt aaattcgaaa catataagca gagctacgaa caccaagact
     4981 gtttcggctc tgttttttgc atcaaattca ctcttatata gctgataatc tcggctcttt
     5041 aaaagaaatt attttcgctt cattttatta taaaaaggct aggaaccttg atgttatcag
     5101 gttcctagcc tttatttaag tattacccca cagacccttc catatagatt tttaaattac
     5161 acactagttt aaactaaaac aaacaaggaa aaacttacca tttaatcaaa aaattagctc
     5221 atatgaatat attagaatgt aaattagact ggtcaaaatc tggtcaaaaa agaggacgaa
     5281 tacaggtagt cctctttttt gttattttga tattctaaat cattttttat tggattattc
     5341 ccttgttcga tatagaccca aattgatcta tcaataatta tcaaactgag tcatatagag
     5401 acaaaaattt caaaaatgac ctgttaaatg gtcatcgaat atcatttaaa atcaatacga
     5461 cggagtcaga cacggagtca aaacaagccc gctgagagta aaaaatatac agtcccatag
     5521 gtaaagtgcc acaggactgt atattttttt atttgagatt acctgaaatg attccattta
     5581 atttttatct cattttttcc tactagttag tatcaaatac tctcctattt tctcaataaa
     5641 ctattttggg ttacatggta tctgaaacag ctaaaacctt gtcattatag caaaaaatca
     5701 ctttatctcc gtttcatttc agatacattt cacttaagcg gattccaaaa aaacaaaaga
     5761 aaaaggaacc cctagttagc ctatgctttt tattggttcc tctcaaaaca cggtcatata
     5821 acaacggggt ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa tagctgtctt
     5881 aaatacttcg ttataaaatg tgaacaaata aaagacatct atccctattt tctacacttc
     5941 cacttgacta ccatctttga atcgtacaat tttcttaact cttctcgttt acctcaattc
     6001 attctcaagt ggaatcaact catcatctaa cattttacgc catgctcgct tcatttcttg
     6061 tgggtattta aatggcttcg cttctggatg tttggcttta tatccttcgt aatccttact
     6121 gttgataatt gctttcatat taggaattgg atcttcacct ggtagatatt gcagattaga
     6181 agcatgtaat tctttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc
     6241 actcgcttgt gtaaaatagc gacgtttaat gacaaagata tcttcattac tatctaaagt
     6301 ccccacatca aatgcattta aaatttgtcg gtaaacttct ttgactcctt ctgttttatt
     6361 caatttagcc aaagcttttt caatttcttc tcgtgcatct gaactattcg cagcataagt
     6421 tcctagtaat tgatcaatat aagttgaatc aatatcatac aacttagtgg tattttcact
     6481 ataaaattcg atcgttgaaa aatcttctcc ttctttttct ggttcttcct ctattaatga
     6541 acccttcacg gtctcgtaca cgccaatttg ttcttccaaa agttgaattt gattgttcaa
     6601 agttggagat ttcaccatgt catcattgta atcgtcataa gtcactaacg attcataagc
     6661 acgattcaat tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc
     6721 atcaatggat tccatggatg aaacctcttt taacttacca tatgcttgtt tgaatctttt
     6781 tttggattcc tgataacttg gataaatcaa acctgattct tcttttgctt ttgaatacag
     6841 ttttgtcgca tcctcgacat tcttctccat tgtcgctggc ttacgaaacg tcacaatcaa
     6901 tccttttgtc ttatcaggat acgtacgatt cgtacgtgaa aaagcttgga tcaatcctgc
     6961 atactctaga ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa aacctgttaa
     7021 tagacgatcc actacaatcg ctaaatcgat ttgtttccca aattgtttga attccgctct
     7081 ttttcgtgcc aaacgattat tgatgtctcc gttataacgc tcaatgtctg ctaatgacca
     7141 ggaagtatca taatactgat tatattcctc aataattttt tgcatttctt cttgttgggc
     7201 acttgaatct cggctatttt cttctaatga ataggtaata gcgattcgtg gaaaatcagg
     7261 atcctccatc gttcgtcctt cacgaatgag ctgattggga aactcttttg ttaaccaatc
     7321 aggctctttc gtcatctctt tgatggcccg ataatacttt ttggccatgt caatggaact
     7381 cgtggttaag attgcagatt tcgttggcct gccatttcga aaatcaaatt ttgtataggc
     7441 attatctgga cggaacattt tatgaatgac tttttggata tgttcatctg cttcaaaaac
     7501 agctgggtcc aaataacttt cttttttgac aggttccatt tgatcaatca tacgattgat
     7561 ctgttctgat gaatactctg catatgtttc tacttctttt aatttccgat agattttatt
     7621 ctctagtgaa gtcggctcca cggtgttttc atgctctact tgaaatccta agacagagcc
     7681 atcttctagc gcattcttaa tggtataggt atgcaagacc tctccatact gatcatgggt
     7741 tgtacgtgcc aattgccctt tggcttgttt ccgattctca ggaaaaatcg gtgtacctgt
     7801 aaagccaaac caagtggatt tcggaaacat ttttttgatt tcttccatat tttcagcact
     7861 taacgctcga tgacactcat ctactacaaa cacgatatgt tggcccatta atttttggaa
     7921 gcgattggtt ccttttttct cttcttgttt ctttgcatat ttcaacgccg catctagttt
     7981 ttgacgggtt gtaatgatta caacatttgc attggcatct gccaacaggg tttcacttaa
     8041 ttctttggca cttcccgttc caacgatcaa gctattcgct ttggcgttcc cggaagaaat
     8101 cccagtatta aattccgaag caaattttgt aaactctgtg gtggtttgat tatctaaatc
     8161 tttgcggtcc actaacatga tcgttcgatc gatccccgat tttcgtgcta aaagcttagt
     8221 agatacaaaa ctagtcaacg tctttcctga accagtcgca tgccacacat accctgattg
     8281 atgtttattt gcggcaataa acaaggcttg aatcgcatgt acttgataag gatgtaacac
     8341 catgagggtt ttattatctt gatcttcact gacgattgta taatccgcaa tcaaacgatg
     8401 agcatccggg atatttaaca cttgtttaca aaactcatat aagttttcta ctttttcatt
     8461 gtctttcgtt cgccaactaa ataaaaactt agggtgcaag tctttcggta aggcatttgc
     8521 aaaataccga gtggtttgtt cattggaaac cacaaacaac tgtaaggtcg aaaagatatt
     8581 gttccgaaac ataccttctt ctgcatattt tttgatttgg ttaaatgcct gataaacacc
     8641 atcttttgca gttacttgtt tcagctcgat ttggacaatc ggtaatccat tgattaataa
     8701 ggtgacgtca aaacgtcgat cccgtgcttc tatgttactt ccacgttttg caatttgatg
     8761 gaccacttca taagtagaga ttcctccacc aatgtcttga ttggaataaa ggataagtga
     8821 gacagaaccc aattgcgaat cttcccgttc aatcgtgatc cgagccatcc cattttcacc
     8881 ttttaaccat ttcgcagcat caaatggggt tttcgttcgc agcagtaatt ccgttttgat
     8941 cgtttcaaat tctttatcgg tcaaaggaaa atcattcagt tctgcttgat tatttgagat
     9001 aattttttgg cgtaaattga cccaaaggtc ttcttctgat tttagatccg gacgatacgt
     9061 ccactgatta tgcccttctc caagtacacg aatcaactgt tcttcgactt ctgcttcctc
     9121 acggtgacta attttcattc ttcttccctc ctagacaaac atcttttgta agaagctttt
     9181 tttcgtttct tttagtaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg
     9241 tttgaagaaa gtaccgattt tttgttgttc atcctttaca ggaaccttaa cactgatatt
     9301 ctcaatatca gaactgttat aagaaggcaa tgcccctact tgggagtatc ttggcaaatt
     9361 tatagtttca aacaatattt taccaaaata gatatcccaa gtattattaa ataaataagc
     9421 catggtattg ttatctatta agaaagggct tgtgaccaaa cgttttctat taagcattat
     9481 tgctgcgcca actttagcaa atataatcgc tggcacatct tttattggct tccatttttt
     9541 acgctcaatt tgctcattag atacataatg atttgcattt ctcatttcat actcattacc
     9601 aatgttattc atgtcggaaa ctttaaaaaa tggagtacct tcagaaccac cttgctcggc
     9661 atcaggaaat ccaattcccg attgagtttt acctaattct cccaacttac gctcttccca
     9721 atcttccgta aaaccaggaa accggatttc tggcactttt gctccgtttt cagggaacat
     9781 tttttgtaag aatccttttt tcgtttcctt cagcaaatct aacttacgtt gatgaagagc
     9841 gatagtgtcg tcgagttttt tgaagaatat gccgattttt tgttgttctt ttaaatcatt
     9901 gggaatatag aatactgtat ctgaaacttt tttccagtct gaacgaggca tttttgttcc
     9961 agatgtatca tttgcagcct tttgatatct atcagcttga ataagagaat aaataaagtc
    10021 cgagtcgctc ttcttagctc taaaaaccca gaaatcccca atcgctacgc ctgtaaaagt
    10081 tgccttgagc caatttttta aatatggacg taactttcca tatagaatat catttggtaa
    10141 aaattcaatc ccttttctat tatcaaattt ctggctcaca tctttattca gtctaccttc
    10201 tcctgccacg atatcttcaa attcaacttt tggaagtcta ctggagtttg tacttttatt
    10261 aagtctaaca actaaatcaa ttaacttacg ctcttcccaa tcttccgtaa atcctgggaa
    10321 acggatttct ggtcgtgtat cattgctcat gtttgaacac ctccagcgca ccttgtaacc
    10381 acatttcgtt ttcaggtgtt gtttgcaggg aagagatagc ttctaaaagt tctttttcta
    10441 gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc gacatgaacc atatcaattg
    10501 gttcttcttc ttcgaaagta tctacatagc gtgggatatt taagttatag tcattttctt
    10561 tgatttcatc aaaggttgcc aaatgagcgt atttctcgac atcttttctt tcagcgtagg
    10621 tttcgaggat tttttgaatg ttttcttccg atagtttatt ttgatttttc cctttaacaa
    10681 attcacgact agcatcaatg aataatacat ctcgagtttg acgattcttc ttcaacacaa
    10741 tcaccgtcgt tggaatcgat gtcccaaaga acaagttcgc aggcataccg atcacagcat
    10801 aaatactgcc atcttctagt aatttttgac ggatgacccc ttctgcagcg ccacggaata
    10861 aaactccgtg tggtaagacg atggccatgg tacctgtttc cttcaaatga tagaacccat
    10921 gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa tttcccataa cgattaaacc
    10981 gagaatcatc taagaaagtc gtatctgctg accaattagc cgaataaggt gggttcatta
    11041 ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc cttattcaac gtatccccat
    11101 tgcggagatt catttcttcg gcgtcgacac catgtaaaat caagttcatt tttgctagat
    11161 tgtatgtcgt tgtatttagt tcttgcccat gatatttaac gttatctggg tgtgttaagt
    11221 agttacgtac gtttaacatc aatgaacctg aacccatcgt tggatcaaaa acactaaaga
    11281 atcgtcgttc cttttgatcg agcgttacga tttgcgccat catatcagaa accatatgag
    11341 gtgtgtaaaa ttctccggct ttcttccctg cttctgaagc aaattgactg atcagaaact
    11401 catacgcatc cccgatcacg tccccatcgt gttctaaaac atctacatca ttcaatttct
    11461 tgatgacttc ggtaatcgtc acatttcgtt gttgctcatc tgttcctagt ttttttgatt
    11521 ggagatccac atcatcaaat aacccattaa attgattgta ggtagatgcc aattgcacaa
    11581 aggctttgtt tagatcgttc aattggaacg ttgcttgttt tgcttgatcc gctaagacgt
    11641 tgaaaagata ctctggtgca attgcatatc ccaaaatatc aacgattgtg gcaatcagat
    11701 cttcttttga ttcttcatct gaaagtaatt cacgatacaa cattgtttgt ttgcttactg
    11761 tgtcatattc ctctaaggat tcatctgcta aaagaaccac ttgttccaac aatcgatcgg
    11821 ataaatattt atagaaaatc agtcccaata aataattttt atattctgac gcatccattt
    11881 tactacggag attatctgcc gcactaaata atttttgatt taattctgca cccaaaaaga
    11941 tacactccta tctcttattt aaagttgcta tataccctct tattttagca tacccccttt
    12001 tataaaaagc aaaaaaaaaa gaaagaaact tattcttgct aataaactac gcgaaaggag
    12061 tctaacgttg tttgatataa cttagactga tcgttaactt attttttata tttctacata
    12121 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
    12181 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
    12241 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
    12301 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
    12361 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
    12421 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
    12481 gttacctgtc cgttcttaaa taccacagaa taatcttttt acccattttc aagtaaccga
    12541 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
    12601 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
    12661 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
    12721 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
    12781 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
    12841 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
    12901 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
    12961 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
    13021 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
    13081 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
    13141 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
    13201 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
    13261 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
    13321 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
    13381 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
    13441 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
    13501 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
    13561 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
    13621 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
    13681 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
    13741 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
    13801 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
    13861 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
    13921 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
    13981 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
    14041 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
    14101 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
    14161 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
    14221 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
    14281 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
    14341 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
    14401 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
    14461 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
    14521 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
    14581 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
    14641 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
    14701 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
    14761 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
    14821 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
    14881 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
    14941 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
    15001 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
    15061 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
    15121 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
    15181 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
    15241 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
    15301 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
    15361 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
    15421 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
    15481 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
    15541 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
    15601 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
    15661 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
    15721 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
    15781 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
    15841 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
    15901 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
    15961 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
    16021 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
    16081 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
    16141 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
    16201 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
    16261 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
    16321 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
    16381 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
    16441 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
    16501 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
    16561 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
    16621 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
    16681 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
    16741 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
    16801 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
    16861 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
    16921 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
    16981 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
    17041 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
    17101 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
    17161 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
    17221 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
    17281 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
    17341 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
    17401 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
    17461 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
    17521 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
    17581 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
    17641 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
    17701 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
    17761 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
    17821 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
    17881 atagccaatt tcaatcattc cgagatataa aggtatcaat agaatgattt gggctagtat
    17941 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
    18001 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
    18061 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
    18121 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
    18181 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
    18241 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
    18301 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
    18361 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
    18421 tgtaaaatct gtgttaccaa tggttttgat gacttctcta atccagatgt acctgtcatt
    18481 tttgaaaaaa taatacctat aatagccaag acaacaatac ctataatgac aaacttcttg
    18541 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ttcttatccc
    18601 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
    18661 atcatcaaaa tcgtacccat tttctcatct ttttctcgat ataagtagcc cataataaca
    18721 gcaacagcac caaatacaac aggaataaat gctaacgata ctaccataga aatccaacca
    18781 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
    18841 attgtctgct cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt
    18901 agtgctccac aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
    18961 caatacatgt cttctcctcc taaaatcatt caattgctta ttagttaact gtttacttta
    19021 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
    19081 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
    19141 acattcattt tttttgctaa ctccgtttga gaaagtcctt tttcttttct gtatcggctg
    19201 agattattta cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg
    19261 tatttcaact cataaaatat aaaaaacttc aaataccatt acaaatacaa caacttgttg
    19321 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
    19381 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
    19441 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaacag
    19501 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
    19561 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
    19621 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
    19681 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
    19741 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
    19801 ccatcatctc ttgttacact tgtaaattga tacagaggaa ctatcccagc tcctgataat
    19861 atcaattctt ttattgtttc atctatccct gcccctttcg ctaaatatat taaaaaacta
    19921 ctacttgctt tcacattgta tagtttctta atatccttag aagctcgttc aagagtgaat
    19981 ccttgtttaa caaagtagcc tctgtttttt attaaagcat ttttcacgct gttatcccca
    20041 acattacgta ggcgttcaca aacagtgtta ataagttctt tctctgtcat tagatatgtc
    20101 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
    20161 ttgctgagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
    20221 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaatttt ttccagaagt
    20281 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
    20341 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
    20401 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
    20461 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
    20521 gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
    20581 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
    20641 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
    20701 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
    20761 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
    20821 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
    20881 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
    20941 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
    21001 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
    21061 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
    21121 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
    21181 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
    21241 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
    21301 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
    21361 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
    21421 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
    21481 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
    21541 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
    21601 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
    21661 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttcttgatct
    21721 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
    21781 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
    21841 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
    21901 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
    21961 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
    22021 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
    22081 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    22141 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    22201 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    22261 acataatacc tcgataaaaa aggggtgtca acagctttgt aaaatttatt tacaacaccc
    22321 cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    22381 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    22441 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    22501 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    22561 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    22621 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    22681 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    22741 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    22801 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    22861 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    22921 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    22981 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    23041 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    23101 gtactccttg taaactaaga ggagatgatt taatggcaaa aatatcaaag tacaaaaaga
    23161 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    23221 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    23281 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    23341 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    23401 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    23461 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    23521 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    23581 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    23641 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    23701 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    23761 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    23821 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    23881 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    23941 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    24001 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    24061 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    24121 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    24181 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    24241 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    24301 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    24361 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    24421 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    24481 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    24541 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    24601 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    24661 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    24721 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    24781 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    24841 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    24901 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    24961 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    25021 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    25081 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    25141 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    25201 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    25261 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    25321 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    25381 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    25441 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    25501 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    25561 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    25621 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    25681 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    25741 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    25801 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    25861 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    25921 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    25981 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    26041 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    26101 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    26161 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    26221 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    26281 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    26341 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    26401 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    26461 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    26521 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    26581 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    26641 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    26701 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    26761 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    26821 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    26881 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    26941 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    27001 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    27061 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    27121 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    27181 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    27241 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    27301 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    27361 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    27421 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    27481 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    27541 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    27601 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    27661 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    27721 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    27781 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    27841 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    27901 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    27961 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    28021 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    28081 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    28141 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    28201 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    28261 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    28321 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    28381 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    28441 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    28501 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    28561 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    28621 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    28681 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    28741 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    28801 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    28861 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    28921 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    28981 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    29041 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    29101 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    29161 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    29221 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    29281 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    29341 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    29401 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    29461 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    29521 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    29581 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    29641 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    29701 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    29761 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    29821 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    29881 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    29941 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    30001 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    30061 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    30121 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    30181 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    30241 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    30301 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    30361 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    30421 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    30481 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    30541 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    30601 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    30661 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    30721 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    30781 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    30841 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    30901 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    30961 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    31021 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    31081 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    31141 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    31201 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    31261 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    31321 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    31381 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    31441 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    31501 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    31561 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    31621 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    31681 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    31741 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    31801 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    31861 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    31921 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    31981 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    32041 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    32101 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    32161 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    32221 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    32281 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta tgatttttca
    32341 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    32401 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    32461 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    32521 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    32581 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    32641 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    32701 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    32761 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    32821 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    32881 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    32941 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    33001 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    33061 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    33121 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    33181 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    33241 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    33301 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    33361 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    33421 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    33481 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    33541 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    33601 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    33661 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    33721 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    33781 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    33841 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    33901 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    33961 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    34021 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    34081 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    34141 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    34201 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    34261 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    34321 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    34381 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    34441 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    34501 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    34561 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    34621 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    34681 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    34741 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    34801 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    34861 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    34921 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    34981 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    35041 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    35101 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    35161 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    35221 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    35281 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    35341 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    35401 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    35461 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    35521 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    35581 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    35641 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    35701 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    35761 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    35821 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    35881 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    35941 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    36001 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    36061 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    36121 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    36181 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    36241 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    36301 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    36361 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    36421 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    36481 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    36541 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    36601 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    36661 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    36721 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    36781 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    36841 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    36901 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    36961 caagag
//
LOCUS       DBNIBO010000009        35712 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.009, whole genome shotgun sequence.
ACCESSION   DBNIBO010000009 DBNIBO010000000
VERSION     DBNIBO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.009"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(275..1330)
                     /locus_tag="AC1Y77_000422"
                     /pseudo
     CDS             complement(275..1330)
                     /locus_tag="AC1Y77_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1502..1636
                     /locus_tag="AC1Y77_000423"
     CDS             1502..1636
                     /locus_tag="AC1Y77_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574029.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(1629..2264)
                     /locus_tag="AC1Y77_000424"
     CDS             complement(1629..2264)
                     /locus_tag="AC1Y77_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574030.1"
                     /translation="MDNRNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2377..3012)
                     /locus_tag="AC1Y77_000425"
     CDS             complement(2377..3012)
                     /locus_tag="AC1Y77_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HIQ1574031.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQIEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(3046..3507)
                     /locus_tag="AC1Y77_000426"
     CDS             complement(3046..3507)
                     /locus_tag="AC1Y77_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HIQ1574032.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(3637..4068)
                     /locus_tag="AC1Y77_000427"
     CDS             complement(3637..4068)
                     /locus_tag="AC1Y77_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIQ1574033.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(4086..4406)
                     /locus_tag="AC1Y77_000428"
     CDS             complement(4086..4406)
                     /locus_tag="AC1Y77_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574034.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            4705..5481
                     /locus_tag="AC1Y77_000429"
     CDS             4705..5481
                     /locus_tag="AC1Y77_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HIQ1574035.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            5496..5699
                     /locus_tag="AC1Y77_000430"
     CDS             5496..5699
                     /locus_tag="AC1Y77_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574036.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            5715..6053
                     /locus_tag="AC1Y77_000431"
     CDS             5715..6053
                     /locus_tag="AC1Y77_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HIQ1574037.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            6040..6219
                     /locus_tag="AC1Y77_000432"
     CDS             6040..6219
                     /locus_tag="AC1Y77_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574038.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6262..6732)
                     /locus_tag="AC1Y77_000433"
     CDS             complement(6262..6732)
                     /locus_tag="AC1Y77_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574039.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            6819..7517
                     /locus_tag="AC1Y77_000434"
     CDS             6819..7517
                     /locus_tag="AC1Y77_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HIQ1574040.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            7695..8036
                     /locus_tag="AC1Y77_000435"
     CDS             7695..8036
                     /locus_tag="AC1Y77_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574041.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            8029..8700
                     /locus_tag="AC1Y77_000436"
     CDS             8029..8700
                     /locus_tag="AC1Y77_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HIQ1574042.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKVNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            8706..9392
                     /locus_tag="AC1Y77_000437"
     CDS             8706..9392
                     /locus_tag="AC1Y77_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HIQ1574043.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            9395..10144
                     /locus_tag="AC1Y77_000438"
     CDS             9395..10144
                     /locus_tag="AC1Y77_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574044.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            10156..10425
                     /locus_tag="AC1Y77_000439"
     CDS             10156..10425
                     /locus_tag="AC1Y77_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574045.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            10430..10594
                     /locus_tag="AC1Y77_000440"
     CDS             10430..10594
                     /locus_tag="AC1Y77_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574046.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            10587..10889
                     /locus_tag="AC1Y77_000441"
     CDS             10587..10889
                     /locus_tag="AC1Y77_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574047.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            10886..11047
                     /locus_tag="AC1Y77_000442"
     CDS             10886..11047
                     /locus_tag="AC1Y77_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIQ1574048.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            11044..11349
                     /locus_tag="AC1Y77_000443"
     CDS             11044..11349
                     /locus_tag="AC1Y77_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574049.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            11349..11705
                     /locus_tag="AC1Y77_000444"
     CDS             11349..11705
                     /locus_tag="AC1Y77_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HIQ1574050.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            11665..11910
                     /locus_tag="AC1Y77_000445"
     CDS             11665..11910
                     /locus_tag="AC1Y77_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574051.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            11907..12326
                     /locus_tag="AC1Y77_000446"
     CDS             11907..12326
                     /locus_tag="AC1Y77_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HIQ1574052.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            12323..12880
                     /locus_tag="AC1Y77_000447"
     CDS             12323..12880
                     /locus_tag="AC1Y77_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HIQ1574053.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            12877..13173
                     /locus_tag="AC1Y77_000448"
     CDS             12877..13173
                     /locus_tag="AC1Y77_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574054.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            13250..13663
                     /locus_tag="AC1Y77_000449"
     CDS             13250..13663
                     /locus_tag="AC1Y77_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HIQ1574055.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            13972..14124
                     /locus_tag="AC1Y77_000450"
     CDS             13972..14124
                     /locus_tag="AC1Y77_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574056.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(14121..14396)
                     /locus_tag="AC1Y77_000451"
     CDS             complement(14121..14396)
                     /locus_tag="AC1Y77_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574057.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            14892..15056
                     /locus_tag="AC1Y77_000452"
     CDS             14892..15056
                     /locus_tag="AC1Y77_000452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574058.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            15252..15419
                     /locus_tag="AC1Y77_000453"
     CDS             15252..15419
                     /locus_tag="AC1Y77_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIQ1574059.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            15445..15789
                     /locus_tag="AC1Y77_000454"
     CDS             15445..15789
                     /locus_tag="AC1Y77_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIQ1574060.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            15794..16075
                     /locus_tag="AC1Y77_000455"
     CDS             15794..16075
                     /locus_tag="AC1Y77_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574061.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            16178..16492
                     /locus_tag="AC1Y77_000456"
     CDS             16178..16492
                     /locus_tag="AC1Y77_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HIQ1574062.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            16470..18164
                     /locus_tag="AC1Y77_000457"
     CDS             16470..18164
                     /locus_tag="AC1Y77_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HIQ1574063.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            18184..19362
                     /locus_tag="AC1Y77_000458"
     CDS             18184..19362
                     /locus_tag="AC1Y77_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIQ1574064.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            19325..20011
                     /locus_tag="AC1Y77_000459"
     CDS             19325..20011
                     /locus_tag="AC1Y77_000459"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HIQ1574065.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            20011..21171
                     /locus_tag="AC1Y77_000460"
     CDS             20011..21171
                     /locus_tag="AC1Y77_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIQ1574066.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            21181..22056
                     /locus_tag="AC1Y77_000461"
     CDS             21181..22056
                     /locus_tag="AC1Y77_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574067.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            22053..22364
                     /locus_tag="AC1Y77_000462"
     CDS             22053..22364
                     /locus_tag="AC1Y77_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574068.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            22354..22707
                     /locus_tag="AC1Y77_000463"
     CDS             22354..22707
                     /locus_tag="AC1Y77_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HIQ1574069.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            22697..23098
                     /locus_tag="AC1Y77_000464"
     CDS             22697..23098
                     /locus_tag="AC1Y77_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574070.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            23091..23495
                     /locus_tag="AC1Y77_000465"
     CDS             23091..23495
                     /locus_tag="AC1Y77_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574071.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            23507..24115
                     /locus_tag="AC1Y77_000466"
     CDS             23507..24115
                     /locus_tag="AC1Y77_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIQ1574072.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            24135..24497
                     /locus_tag="AC1Y77_000467"
     CDS             24135..24497
                     /locus_tag="AC1Y77_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574073.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            24500..24682
                     /locus_tag="AC1Y77_000468"
     CDS             24500..24682
                     /locus_tag="AC1Y77_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574074.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            24699..28130
                     /locus_tag="AC1Y77_000469"
     CDS             24699..28130
                     /locus_tag="AC1Y77_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HIQ1574075.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            28181..28918
                     /locus_tag="AC1Y77_000470"
     CDS             28181..28918
                     /locus_tag="AC1Y77_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIQ1574076.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            28928..31219
                     /locus_tag="AC1Y77_000471"
     CDS             28928..31219
                     /locus_tag="AC1Y77_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HIQ1574077.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            31243..33369
                     /locus_tag="AC1Y77_000472"
     CDS             31243..33369
                     /locus_tag="AC1Y77_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574078.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            33386..33535
                     /locus_tag="AC1Y77_000473"
     CDS             33386..33535
                     /locus_tag="AC1Y77_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574079.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            33532..33978
                     /locus_tag="AC1Y77_000474"
     CDS             33532..33978
                     /locus_tag="AC1Y77_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574080.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            33980..34117
                     /locus_tag="AC1Y77_000475"
     CDS             33980..34117
                     /locus_tag="AC1Y77_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HIQ1574081.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            34155..34448
                     /locus_tag="AC1Y77_000476"
     CDS             34155..34448
                     /locus_tag="AC1Y77_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574082.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            34445..34669
                     /locus_tag="AC1Y77_000477"
     CDS             34445..34669
                     /locus_tag="AC1Y77_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIQ1574083.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            34666..35691
                     /locus_tag="AC1Y77_000478"
     CDS             34666..35691
                     /locus_tag="AC1Y77_000478"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIQ1574084.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        12524 a         5489 c         7215 g        10484 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgtgt atgccttagt ccatgcaaag taaattgctt ttcaagccct aattctttta
      421 attttttgcg caaatatttg ctgacggcgt ttgatgaaac taatccattt tttaagttga
      481 aaaaaacaaa attatgaggg tttttgattt caaaattctc gaataattct ttttgattga
      541 ttttaaactt ttttaagaga tcaatcgtat gtttatcaat ggaaattttc ctatttgaag
      601 tttcgttttt tgtttttccc cattcattta atttataatt ccatgtttta ttaatagtga
      661 tgatttgatc ttcgaaatcg atatcttccc atgttaatcc tagaagttca gcaaatcgca
      721 ttccagtggc accagcaact aacacgagca taggagaaga gtactgcgca gaaaggtttt
      781 cttctacgag tttcattaaa gatttaaatt gatcataatc taaatattta tcttcttttg
      841 gcttcaatga atcttttcct ttgattactg cttttctagt tggatcaaac ggaattaatc
      901 cttcttctac ggcatctttc aacgacgctc taatatggtt attaaactta ataacggtag
      961 attttacatg gtctttagca tacttattta aaaagcgttg atatcccact ctatctaaat
     1021 cagaaatcaa taccgctggc atatatttct ttatgttcat taacgtatct tcgtattttc
     1081 tataagtgat aggagatact gttccttctt tataaagttg catccaatcc tcaaagtaat
     1141 ctgataataa tagatttttg cgatcccctt taaggccttt gttcaagtta tattctaatt
     1201 cgttggcggc atcttttgct tctccttttg tcttaaatcc tgattttctt atcttcgtgt
     1261 attttccgtc atcttttttg tatgaaattt cgtattgcca actatttcca cgtttgacta
     1321 atcttgccat aattgaatca gctctctttc tctgatacaa taggaactat aaagaagccc
     1381 attgtttggg ttttattatt ttttgcacat cctttttgct tgccggcgca aggatgtgct
     1441 atttgtcgtt ttaatggtat aattggatgt gtattagatc atccaatcag aggtgtttat
     1501 tatgattaaa gtgattttta aaagaataac tgaacttcca gagtttagat tatttgttga
     1561 ttattggtat gtctggtttt ttattgttgc attgctgata ggtctagatg tcatattaga
     1621 aagaaaactt aagtaagagt tgagttaata aattgaattc ctaaatgaga tattttgtag
     1681 cccatatttt gtttgccgac atcacctaat actatggagc taatcgtttt ttctaacaga
     1741 ccaaaatcag ataatctatt taagctacca tctattagct cttgtgaata gccagacatg
     1801 ctgatatttc cagtgaaatt ttcgtttcca cgactcatta agcatgctaa caattgaatc
     1861 tcaatcgatg ttaattgatt cagtacaaga agataatact cctcgttatc ccatgaagcg
     1921 ttgttaattc gaagtaaaca attcttataa agatttttat acatatctat tttattttgt
     1981 gcctttgatt tttctacctc gtcatttatc ccttcgaata ttccaatgag ttggtccttg
     2041 ttctctaagt tgaaaacatc ttctggaatt tgatcgcgaa tgttttctaa tgattcgttt
     2101 aaatttttgt agaatttctc gatgcgtttg aaacgcttct cgtttttaga cccaaaataa
     2161 agtgactgca caacgccacc aatataagga attgctccaa taccgccttc aactattaat
     2221 tcaagatatt cttttttggt tagtttttca tttctattat ccatagttac ctcctatttt
     2281 gtcatttcct ttacaagcat tgtgactcct gatggcactc ttttgtatag gttttattat
     2341 tgtgcacaat ctaactctta gtggggcgtg gttcatttat tttttactta ctttactatt
     2401 aatcatatcc cattgagcat aggatttttc tatgttcgat cctcttgaac taatttctcc
     2461 tgtaaaagtt actttatcgc cctttgtaaa ggtatcagca ttgtttaagt ccttcgcaaa
     2521 aattacatat gaaactttag gtgttgcaga aatatcattc caacttttat catcgtactc
     2581 tttgtctgct ataatggcta ttctagtagt cattgattcg ataacagttc cttcaaaagt
     2641 atatgtttta ccactaatat atttagtaaa agctgtattt tgctcagtag ctggagtaat
     2701 agaataataa ctatctaaga aagcacccca gtcatgatta tttgtctcta aaaattcttc
     2761 aatttgagtt aggcttttat tgctgctgtt agatgtagta gtttgagtat cagatgatga
     2821 gctttcctgt ttgttattgt catttgcctc gtttgatgag gaaagcgaga aacctacggt
     2881 agtaaaaata atcccgataa taaggagaat cataggtgtt ttcttatttg tttttttgat
     2941 aaatcttaca attaacatga ttaaacctat aataaataaa acaataccag taaaaccaaa
     3001 tataccgtcc attaatagat cctctttctc ttttgatatt gaatattata aaccaagcaa
     3061 ttcttttttc ttaaaatcga attcttcttg attaataatc ccttcatcta acaactcttt
     3121 atacttttta atttcatctg cacttgaaat ttgtgtttct ttggaattat tatctaatat
     3181 agattgttgc ttctctatat atttttttaa attttccatt tcattccaat atttttttgt
     3241 aaacataacg gtgttttcat ctttcgtggc cgctaatata cctccgcgac tttcgtttcc
     3301 gcctagcaag gtaaattgta tataaccatt actcatacct ggttttttta attgtacggc
     3361 tgatatattt ttaaagggga tagttttttc tcctttaata ccttgattaa ctaaatttat
     3421 aaatcctttt ctaataattg aaatagaatc atcagttact ttaatcaagg tttttcctgg
     3481 agattttatt aaaatctctt tttccatttt ttcttcctca tttctgtgat atgatttttt
     3541 ttgtagaagc tcatatatga ggaaagagcc cgtgttgcag cacgggcttt tttaatgttt
     3601 tgctgaaatc ggttttttta ataattcttg atgtattcat acatattgcc ttgcgtaaga
     3661 atattttttc ttaaaatagc attggcagac agcataacaa gtgtatctga gcttattctg
     3721 aatagaataa tactccataa atttttcgag attaaactga gattcatcag tcagttcgtt
     3781 ctcaatatag atatttaata aaattagaat agctatttta tctgcttctg tttcaaattt
     3841 tgagtgaaaa gttgtagaag tatcgtacaa aactgaaaat tcaaaatgag aggcgctgaa
     3901 atgtgcaagt tcatgagata aatgaaaggc ttctgcagtt tcaccgtata gattttcatt
     3961 caaaaaaatg attctgggtt ttggatagta gaaaccaggc tctttcatct ccatataaac
     4021 tactttcaaa ttgtattcgc tcaacatttc tttcaatttc aaatacatac aaaccatcac
     4081 tccaactatt cattttcctc taaagcttta gcaattgcaa tcgctttacg cattgtctcc
     4141 ttagatattt cttttccatc aaaagaaaaa acagtatcgt cttctgataa atccacatgt
     4201 ttaggggttt ctctttcttc tctacctaga aggtagtcta cagagacatc gaaatagtca
     4261 gcaatccgac ttaattcatc tgaattagga gtattatttt tccactttgc tagataaccg
     4321 ttagaatatc caaaattaat ttctaactgc cgtatagata tctttttcct cttcgccaag
     4381 tcttttatta tttcataggt attcatcgat aaaacaacct ttctaaatgt ttacaaaaaa
     4441 agtttagaaa aatacgcaga aataatttga ctaattctga gtaatgagct atactatatc
     4501 ttgtaaacag atttaacaac taaaaagaca acaaaaaaca ctattgatat gtaaatgcag
     4561 accgccaaga aagctttaaa atcaatgttt ttatgtctta tttaattatg ctcttagtat
     4621 agagtattac tcagatactg tcaattgaat ttagaaaaaa gttgttaaat ttgtttacga
     4681 atataaaaga aaggaatgag aaatatgaac acaccgcaaa tttttaattt cgaacaaaac
     4741 gaagttcgga caattttagt aaatgatgaa ccatattttg taggcaaaga cgttgcgagt
     4801 gttttgggtt attcaaacac taaagatgct ttgtcgcggc atgtagattt ggaagataag
     4861 atggggtcgc gaatcacgac ctcaggtcaa tcaagagaga tgacaatcat caacgaatct
     4921 ggtttgtaca gtttaatctt aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg
     4981 gtaacaagtg aagtgttgcc agcaattaga aaacatggag gttatctaac tccagaaaaa
     5041 gtagaagaag ctttgcttaa tccagataca atcattcaat tagcaactca actaaaagaa
     5101 gaaagaactg gaagattaat cgcagaacaa aagattgcag agtacgaacc taaaatctcc
     5161 tatttagata gcatattatc ttctacagat tcagtaacaa ttagtcagat tgcagcagat
     5221 tatgggatgt ctccacaaca gatgaataaa ttacttcata aactaggtat tcagaaaaaa
     5281 gtcggtaacc aatggttatt gtgcaaaaaa cacatgaacc aaggatacac aaaatctcat
     5341 acaactgaga tcccgaaagc cgatggtggc actaaaattg taatgaatac caaatggaca
     5401 cagaaagggc gtctatttat ctacgaatta ctaaaaaaag aaggatatta ccctcaaatg
     5461 gatttagagg aaattggtta gaaaggagtt ttagtatgac tgacattgca gaaatcactc
     5521 aacgagatag agaaaaaatc aaagaatatg tcgaaagttc aaagttctta acttacacca
     5581 tgcttgctga aagatttgga attagcaaaa gctacttatc tttaatttta aacggtaaaa
     5641 agacttctgc agaagcaaac agaattatag attcgattat cactatgtac gaattgtaaa
     5701 gggaggaaca gctaatgcaa tatctagaag caaaaatccc aattccagaa ggctatgtaa
     5761 ttatctccca agtggattat gaggagttaa aaaaagctga tgatactggt agatggatga
     5821 cgttgccaga agtgctagaa cggattaaca gaaaatatga ttggtttact tctagagttt
     5881 taaagaaccc aagatataga aatattatcg atatcgagaa aaacaaaaat ggatttgtct
     5941 attatccagt tgaaggaaga gacacatatc tatttttaag aagtaaaaca cttgaatttt
     6001 tagaaacaaa tttttcggaa atcttaagga ggcaagcgga tgggaaaatt taatagagcg
     6061 ctagtgttca gcgcaccgct aatcatctac gctttaggac tttggggaag caggcaagcg
     6121 ttgataggaa cgattgttta catggtttgg atttttatgg ggcttgatga agctgaagct
     6181 gagtacagag cgaaaaagcc aaccgagggg gctgactaaa aatgaataga aaagagaaac
     6241 tagaatgtat attactatta ctcagtttaa ttctttcact aatttctcta ttgggaagtt
     6301 tgtatttttg atatcaacaa ttttgtggat ttgggttcta tttagagtaa atatcatttt
     6361 tgtttggtga ttaaggagtt tattaaaatt aattttccct acttcaaatg caaggtaaca
     6421 agatatagca cttttaggag gaatatttag cggtacagct tcagagtata ttctattatc
     6481 atcgactgat cctcctttag caattaattt cttaaatgga gtagctgacc atctgcggtt
     6541 taagttagcg gatgaaagtt ctaaattaac taacatagct ggttctgttg aatagttaga
     6601 aatgattact tttgtataaa tcatatctgc agcgaagtaa gaagcattaa gttcaacttc
     6661 tagttgaggg cgcattcctt ttatttttat ccctgttaaa aacgtactca atataaaact
     6721 tattattgac atccattgaa aaattgttaa gtgaaaaaaa ttcaaaataa acaccaccag
     6781 tttttaacta aattatacca aaaaggagag aagaaataat gcaagaatta gtaattttga
     6841 aaaataaaga agctgtgact acgagcttac aagtggcaga aagcttcgag aaaaaacatc
     6901 aacatgtttt aagagatatt gatgcactaa aaaaagatgt gtccaatttt ggacagatgt
     6961 ttgtagaagg taatgaacca gattcatatg gcagaaatcg acgagttttc ttcattagta
     7021 gagatggatt tttcttgctg gctatggggt ttacaggaaa gaaagctatc tacttcaaac
     7081 aaaaatacat tgaagcattc aacgaaatgg aagatgttat tcgcaagaat actgttcctc
     7141 aaacaattga agatatgatg atctatcaat tagaagaaat gaaagatgtt aaaaaagatg
     7201 tttccatgct taaagatact atgcgaatta gcggacaaca agagtttgaa attaagcaaa
     7261 aaggaaatat gaaagttatg ggagttctag ggggaaaaga aagccgagct tatgaagaaa
     7321 tcagcaaaaa agtattctca aaattttggt ctgaatttaa acgtaccttt tcaatcccaa
     7381 gatatggcga gttacctcgt aagagattcg atgatgctgt ttcatttatt gaaatgtggt
     7441 tgccagaaac tgcgatccgt atggaaattg atcaactgaa cagacaacag agactttttg
     7501 gtgatgacaa tgaatagagc tgaagcgcta agaataggga cggcaattgc taattgctgg
     7561 tggaaatact ataaaccaat catcctaagc caacaacata ttgacaagca aaaagcatgg
     7621 caacaaataa aaaagtgact ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa
     7681 ggagatttta gcatatggaa aaagaacttt ccactctaga tcaatatttg attgatcctg
     7741 attggggcaa gccgaaaatt gaggaaacaa gtggtcgaaa aatcagacga aatcttttga
     7801 cgaatgaaga actagcttgg gatcaagatg atttaggcaa ccatgtaact atttgggatc
     7861 atgtttatct tatccatcta tcgaagcatt cgaataaacc tgaatatatt tacgtcatcg
     7921 aagatggctt gattgatgcg ctagaagagt acgacagaga taacttgatt gatatctctt
     7981 attacggacc aggtaagaaa tacattgctg aaatggaggc agaatttgat gagtgaaatc
     8041 aaagggacaa cgaactttga aaaacttttt agtcgtaagt taaataaaat tctcaagaaa
     8101 aaagtaaatt ttgattattt atcttgggct cacgcgtggg agattatgaa aaagaatgat
     8161 ccacaggcaa cggtaactat taatgagtac aaacactaca gggttgtttc tggaactcat
     8221 caagactttc ttgttgagga atataaacct tttcttatgg atgaaactgg gacttatgta
     8281 tctgtctcag taacggttaa aggacacacg gaaaccgaat tatttcctgt tttagattat
     8341 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
     8401 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
     8461 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
     8521 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
     8581 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
     8641 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
     8701 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
     8761 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
     8821 acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg
     8881 tacagcaacg aaacttcatc tatgcgctga taggcgatat ttttatcgat acaggcatgc
     8941 caacggactt ctggaaggaa ttcttctact ttcgttttga aggtgtcaca gggcgcaaaa
     9001 taagcctgaa agacgaatcg aatacaactg tgagtgatgc caatgtctta gcaaatatca
     9061 tcttagattt catctttgaa catcatattc ctttcaaaga aggctatgag attttacctg
     9121 cgaatcaaga atattacttc tacaaatgca tcacaaaaag agtctgctgc atctgtggca
     9181 aaacaggagc tgacatcgat cactttgaca aagcgctagg aagacgaaag cgcaaagaag
     9241 ttgatcatgc agagtacaca tttgcagcac tctgcagaat ccatcacaca gagaagcaca
     9301 aaataggtgt gattaatttc aaaaataaat atcaaatcaa agggatcaaa ttaaaccaga
     9361 aaacaatcaa aaagttaaat attggagggt aaaaatgaca gaacatcgaa gttattatgc
     9421 gattatacca gccaacgtaa ggtacgacaa aagacttaaa ccaaatacta agttgttata
     9481 cggagagata acggccttgt gtaatgaaag aggcttttgt tgggcaggca atgagtactt
     9541 tgcagattta tatggtgtga ataaagagac catatcgcga tgggtaagtg atttgattaa
     9601 gtttggatac ttgaatcggg aaatcattta caaagagggt accaatcaaa taatcaatag
     9661 gtacctacga attaatcaat accctattga cgaaaaacgc aataccccta ttgacgaaaa
     9721 agtcaaagat aataatacat ctattaataa tacatttaat aatacaaaag aatatataag
     9781 agagttaccg ccttcgaaaa aatcgaaggc taagcccgtc cgtcataaat acggagagta
     9841 taaaaatgtt cttttgtcag atgagcagat ggagaaactc aaaacagaat tccctaatga
     9901 ttaccaagag cgaatcgaac gactgtcaga gtattgtgaa tcatctggta agacttataa
     9961 aaactatttg gcaactattc gaagttgggc aaggaaagaa aaaagtgaac ctaagaacgc
    10021 aagcagtgga tacaagcgca caggaagacg agagaagctt cctgaatggg caatcgacca
    10081 agaagcctat ctcaagaaaa aagcgctaga acgagctaat agacaatcaa aagcaccatt
    10141 ctaagaggtg gaaaaatgaa gatcgattat ctagaactaa ttaatgaaat agcgaattat
    10201 aaaaagggcg aggaattaga cgtcctgaga gacgtatatg atcaactcga agaagctgga
    10261 attgaaggaa ttaagaatga tcgttcgagt tggagtaagc tcagatacta tttcgcactt
    10321 tatatcgata caacacaatt aagaaattta gcatatacca aattactatt tgttgattgt
    10381 gttaaaggct tgcaaaaaca tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa
    10441 gacatttaca gcacagattg aactaatgca tcaagaagct ttaagacaaa gtgtatcgta
    10501 cgaagacaag tggctcaaca cgttccatgg cggacgtgag agcgcacttg atcaagtact
    10561 caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg
    10621 aattgactta ttcagaagat tggcaaaatg acaaagaagc tgcttcagaa gtgatgaggc
    10681 ttggaagagc gatgtgggca gacaagagca acaagccaag accacgaaaa atcgcaattt
    10741 ggcacggtga caaacttcta gtgataggga cagctgaaca gttagcaagt ctcacaggct
    10801 tgcacgagaa aatcgtgagg aaaagagcaa ggtgtggcta cacagacgct aagaagagaa
    10861 cgtttagata cgtggaggaa tcatcatgac aacagaagaa gtgattcaaa tgcgtattcg
    10921 aagccttcag cgtgagattg acgatctgga acgaacaaag gcagtgatgg tcaatgaaac
    10981 ggcgagaaag gcaatcgatt tgcacataga gaatttaaga agggaaatcc atcgattgga
    11041 ggaatgagcg tggataagaa agcagcaatg aaaagaattg ctgaattaac caagtcagaa
    11101 tcttggcaag aagacaaaga aatagttgca gaagtccaaa agctcggtaa atcaatgtgg
    11161 actgaaaagc ccaaacggag aacgccgaga aaaattgcaa tctggcatga tgaccgaatt
    11221 ttagtaacag gtactgctga acagttatct gaaattactg gattaagcaa aaacattatc
    11281 tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag gtatgtggag
    11341 gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa atcatgatga
    11401 agattcagaa agataaaaaa tcaaaagaac gagctgaatt agttaagtta gaaatggctg
    11461 aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac attgaatttt
    11521 attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg caaacaaatt
    11581 ggagcaagaa acttcatcaa gatcgtttca aatttgtaga gaagtataaa gaaatattag
    11641 aagagtattt gaggaggaca gcaaatgata aaaaaactcg ttcaattcag catggattta
    11701 tatgatatcg aatcaggagc aacactatct gtggaatcgg accatctaat cataaatttt
    11761 ggtggaaagc gccagattat tttgtgggta gttgatgatg tactgtttcc agaaattgtt
    11821 catgatttcg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt aatggaattg
    11881 attgaaaaat acgaggagga cagcgaatga taccgaagtt tagagcgtgg gataaacgaa
    11941 agaacgtaat gagagatgta gccgtcttgc attttactaa aaacggcaaa acaaacttta
    12001 ttgaatattg gataaatcct accgaattga aatcatatca cgtgcgaaac atcgacctca
    12061 tgcaatccac aggaatgaaa gataagaatg gtgtggatat atttgaaggg gatatagtat
    12121 tagtcagcgt gcgaaatggc ttcgattact tagataataa agtctgtatt gtcaaaaatt
    12181 caatagatta ttccggatta gtttgtgcca ctgttgatga agacttagag tatcaaattt
    12241 ttaacacaga gctgtttgaa gaatacacgt acgaagtcac cggaaatata tacgagaata
    12301 gcgagttatt ggaggaacag cgatgaataa acaggaagca atcaaaaagt tagaaagtat
    12361 taaagcgata ggaaatgatg caatagctgc ttgctataac gagagtataa attcaggtat
    12421 tacgttaatg aaaaaaatag acgaaccgca gaaaccagtt attccacaac tcgtggccgg
    12481 ttggcttgag aaatctacgg acccttttac aaaagctgaa aaaatagcgt atttaatcaa
    12541 atctaaagat ggtgattcat attatttctg tgattggttt gtacgagatg gcatagtgac
    12601 gcaagagcaa ggagaagaat tactcgcttg ggcaacaaga caatcatatg aaacactatt
    12661 gagcctatac aacggctacg aggttgagaa agagccgtta tgggcaataa agaatgccga
    12721 tggaaactat cttactaaat gtgctttatg gggaaaagat ggagtaaatt atagttttga
    12781 atgcaatcca tctcatcgat tgcttttcac tgataaagca acagcggatg ctgcagcatt
    12841 gttggtgaat ggaacagtgg aagaggtggt agaaagatga aactaaaaga cggattttac
    12901 gctagcagtc acggtatcgg cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca
    12961 cgcaagaaat caaaattcaa agtcggtgac atggtccgct gtgaagcaga agagttcgtt
    13021 tatccgttca gaggatacgt taaaaagata ctgtcaaact cagcaatcat tcgtattgaa
    13081 aatacaatga agtgtgatcg ttcgactgca aaacacaagc attatttagc tgtagcgaga
    13141 ttagttgaca tggaagtaat caagagcaaa taaaaaaagc cggatcgctc cgactgattc
    13201 aataaatcca acacatttat tatatcacat aaaggagcgg tttgacttga tgcaattgtt
    13261 acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg
    13321 gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga ttataacgga
    13381 tatgccgaag gcaccgaagc atggcaataa ggcagaagac gcgattattc agatgatgga
    13441 tatagaaacg gagagagatg cgattctagc ggctttgatg gctcttagtc tgattagtcg
    13501 tcagatactc tactacagct tctgtgtgcc agatagcttc tcaaactaca gaattagccg
    13561 tgaagtgggt tattcagaaa gaagtataca acggatgaag tcggaagctc taatagagtt
    13621 tgcagaagca tataaacacg gaaaaataat tgcttataaa taatttggcg gttttttggc
    13681 ggaatgatgg cggtttttag acatttacca gtgttattat ggtagtgtcg aaagattagg
    13741 aaacaggatc gacaaaataa acgaagggag gcaatctccc tcatcgtttt aaacttcttc
    13801 ttagatagac agcaactaat tatgaagaaa aggatgtgaa ttcaactcct cctaaattct
    13861 tcatcggttg ctgtttattt tgattagaca gcagcactca caaaactaaa catgttcttg
    13921 tcgtctttca actgctgctg tctattaatt tatgtattgg agggaagaaa aatgaaatta
    13981 tcagtagaag gcactccaga agaaatacaa gaattgctcc aagctattgg tagtagcaag
    14041 gagcaaaagg taagtgtttc taattcagat attgacggta tttgcaattg tttaaacaga
    14101 gaaaaaatat ctaatcctta ctaagagaaa atatgttcgt taatgaattt ccaattttcc
    14161 tctgtagctc taccttgata atttttttgc agttcacaga tgaataagga atcgttatta
    14221 tcaagtactg tatttaattt ttcacacatt tgatttggat tcaaattagt tttaactaac
    14281 caaaaagatt tttgtagctt tatatatgcc ccaaattctt ttattatttc aaatacttta
    14341 tcatactttt gccctgggtt attaagatca taactaatca tgtagggttt atacatgttg
    14401 tcttgcacat ccttgtttta tatttcagcg gaccactcgc tgataaataa aattatacgc
    14461 ttagtattta ttttcacaat attaatttat cgtaataact tttgagtaaa tagaacaaaa
    14521 aacctgcact agtttccgct agtacaggca gtgactatat cggttgataa tctagcatat
    14581 attaaaaata attgcaagaa aagaaattta tagtggttta taccaaatta tcaaagcaat
    14641 gttattttgt tgctgtctat tgttttttaa ttattcacac gataactaaa ggtgggtgaa
    14701 gagaaatgat tccattaata atttcaattt ttgcgctctg tcttaatgtc tatatgattg
    14761 gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact
    14821 gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc aaccatgcaa
    14881 ggttcgattc cttgccagcg acattaaatg cctatgacgg ttacgactac cgaaaaaaga
    14941 tcgttaagaa gctatacggt gctacgtacg gcaatgtagt aagtgtgcta tctgtacacc
    15001 accaagcttc ggtcactgtg gcggaagtag aagacgcagc ggtaaatggc gagtagcctc
    15061 gtgagagcct ggtaagttct cgtgagtggt gcaatcccac tccagcgact ttaagcaacc
    15121 gaggcatcgg cggtttaaaa atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt
    15181 gcaagttact attacatatt agatcactct ttgagtggtc tttttatttt tgcacaaagg
    15241 aggaaacaac aatgtataga ccacaatact tagaacagaa gtatgaagta atcactgttc
    15301 ataaaggtaa tggcgaaaaa gtatatgagt atagaagacc aataaagagc gatacatata
    15361 aacgaaagga aaacaatgaa gttattccat tgtatggcaa aagaatagct aagcattaaa
    15421 taagattgcg aaaggagacg gaacatgacc gaggaattct atagatggct attacagttg
    15481 acaagagaag atcgtttggt taagttctat cagtctccta aatggcgcag gcttagagag
    15541 aaagcgatga aacgagatca ctatgaatgc caagagtgta gaagactagg taagtatcat
    15601 agagtagaga acgttcatca tataaaggaa gtcaaggata gacctgactt agctttagat
    15661 ttagataatc ttatttgttt atgtgttgaa catcataatg aagttcatgg cagatatctt
    15721 acagcattag ataaacaaga gaagaagata gaaagctttg ctaacttcga tgcaagtgaa
    15781 aggtggtaag tgcatgatca tcaatgataa tggcagagag tatgatacag aaaagattga
    15841 agagtattca tcttatactc agggattaat taaacgtttg atatacgttc gctatgtagg
    15901 tattagggat ctgttatcag ataactgttg tagtaaatac aaagtgaatc aagtaagaga
    15961 agcgttgaat aaagataata acgtcgaaag aataaaaaat gtttttggat atagtattga
    16021 agagattaat tattacattg acttcgctga agctttcatt ccgatggtga gataaccccc
    16081 ccttaaaata aatcgcaaat tttttggggg tgatgaaacg gagggggctg tcaggaaaag
    16141 agattttttc gaactttatc atgaaaggag ggctaaaatg tttaaaaacg aattgtctca
    16201 aaatcgctac agagaaaaat tacgccgctc tttaataagc caattggaaa gtcagaaaac
    16261 aaatattgag ccattcttag ataatgttga tcgttatatc agtttatggg aaacggcgat
    16321 atcactggaa gaagatatat ccgagaacgg cattagactg gagaatggta aaaagaatga
    16381 atcagtagcg ttgcttgttt ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc
    16441 cattactcct gaattggtag gtgaagcaaa tgaatcaatt cctgagttat aagcatattg
    16501 aaaattggtt caaagctata gaagaaggca ctatcaaggt atgcaaagag caattattgc
    16561 taaaaaatta tctagaagaa agagtcttta ctagagaaga tatttacttc gataagcaga
    16621 tggtagagga ttcaatcaat ataccagcac aatactttcc attcgaatta attccgtggg
    16681 aaaaatttct acaatgtttt atttatggtg ttcgatggaa aaaagataaa acactagtgt
    16741 tcaatagata tctttcatta atgggacgtg gtaatggtaa aactggtttt gcttcttgga
    16801 acaacttctt tctactaacc gctaaacacg gtattaaaaa ttatgatatt gatatctatg
    16861 ccaataatga aagccaagca aagactagtt ttgatgatgt atttaaagta attaaagatc
    16921 atcctgattt agataaaaaa gtatttaaag ctacgaagga agttattcaa aatatcgcta
    16981 caaacagcaa acttcgttat aacacggcaa atgctagaac aaaagatggg aagcgaccag
    17041 gtgcaaaccg ctttgatgaa attcacgaaa atgaagatta ttcaatgata aatgtggcta
    17101 cttctggtgg tggtaaaatt cgagattata gagaatttta tgatacaact aatggtcatg
    17161 ttcgtggtgg tccgcttgat gacattatag aagaatcaaa aatgattctt tctggagaac
    17221 ttggaattga caaggatgga gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg
    17281 ataacgataa tgaagttgat gatcccgaca tgtgggaaaa agcttgtcca actattaatt
    17341 acaatgcaga tttaaaacgg aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg
    17401 gtttaagact tacgttcatg accaaacgaa tgaacagacc tatggaagat acacgatttg
    17461 ctgttgcttc atatgatgat gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg
    17521 atgaagtgat aggaacagtc gattttgctg atagacgaga ttttgccagc gttgggttgc
    17581 taggaaaata cgataaagat gtttatttta cacaacatac ttttatccac gaatcagccc
    17641 ttcgattaca aaacatcaaa cgagaggtta tagatatttc tatagatcaa ggaaaatcac
    17701 agatcgttca tggaaaaaat atagaagctg attatattgt aggttggttt cttgaaatga
    17761 gtaataaata ttatattaaa aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc
    17821 ccgctttaga agaagcaggt tttactgtgg aaattgttcg aagcggatct gttacacatg
    17881 gtatgttaaa agatctggtt gatgaccttt ttattaatca acgtttattt tttggtgacg
    17941 atgcgattat gcgttggtat tgcatgaatg tatatgaaga gcatatttct aatggaaata
    18001 tacgctatga aaaaatagaa cctgaaacta gaaaaacgga tggctttttt tcattccttc
    18061 atggtttgaa ttttttagat gatatttatg attctgctcc tgtaacagtc acaaatagct
    18121 cagtagaaaa tacaggaact ggatttactc ctctagtatt ctaacttgaa aggaggtgag
    18181 aaagtgggga tttttcaaaa ggcggtagga tacttcacaa aaaaagcaac ggttccttta
    18241 gaagaatact tttgtaaatt gcaagttgat tttgtgtatc gaaaatttgc gattgaaact
    18301 tgtattgatt tgattgcaaa tgcgatgagt aaagcggaat tcaagtcata tgaagatgga
    18361 aaaaataaaa agaatgatct ttactatagg ctgaatgtag ctcctaataa gaaaaataat
    18421 gcaacagaat ttagaaaaaa actgatcagg agattaatat tctacaatga agtattgatc
    18481 gtttctccgt ctaataattc tagcgaaata tttattgcgg atagttggga tgtcacagaa
    18541 tatgcattga aagatgatgt gttttctcaa gtgcaaatta acaacatagt ccttgataga
    18601 gaatttctag aaagtgatgt tatctatata aaatacgcag atcaacaaat taggcaacta
    18661 gtcgatgcgt attatcaagc gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag
    18721 cgctctaacg ctcgtagata cgtactgaaa gggaatttat tccgaccgca agacaataca
    18781 acacaagatc aaatcaataa aatgatgaca tcacaattta aggcttttat ggaagctgat
    18841 aatgcaggtg cggtatttca attacaaaat gagtacacat tagaagattt cagcggaaac
    18901 ttccaaagca attcaagaga tataaaaaac ttaatagacg acatctttga gatgacagca
    18961 gcagcgtttc acgttccgaa aaacctacta aagggagaca tgagtgggtt atcggatcaa
    19021 gtggacgctt ttttaatgtt cgaaatcata ccgattgctg aacttattca ggatgcgttt
    19081 aacgctagtc tctatgaagt agaagaatac ttgtcaggga attttgtacg tgtggataca
    19141 actatgatca agattactag cttcaaagat ttggttgacg ctattgatgt aggcattaga
    19201 aatggagtat ttacaatcaa cgaaggaaga gaacgcgttg gaaatgatcg ctctgataag
    19261 gcgatggcag atgaaatatt tataactaaa aacaatcaac aagtatcgaa aggaggtgag
    19321 gcgaatgacg acaatgaaaa catttctagc agtaaagaat gaaggcgcag taccgcaaat
    19381 ttttattcag ggatttattg gttctagttg gttctttgaa gggaatactg acaagggaat
    19441 caaaaatatt ttggatagtc taggtgatca agaagaaatt gaagtagtaa ttaattcaaa
    19501 cggtggagac gtatttcaag ggattgctat tgggaactta cttaagtcaa ataaagcaaa
    19561 agttaacgtt gtgattaacg gcttagccgc tagtgctgct tcaattatcg caatggctgg
    19621 cgatactata aaaatctaca acaatgcaca attgatgatt caccgcgctt ccacatacgg
    19681 agaaggaaat gtcgatgact tccgtacgat tgctgaccaa ctggaatcaa ttgataaatc
    19741 ggtaaaggct tcatataaaa cacgattcaa tggcacagat gaagcattgc aagaacttct
    19801 tgaaaaagaa tcgtttatgg atgcagaaac agctttgagt tatggattgg tcgatgaaat
    19861 tatcgatgca gaaaatagct caggtactga agctaaaaaa gaacaaagcg ttgaagaaat
    19921 tttgaatgac gttaaagaaa aaagagcaga aaaaattgct gcatttacag cagcattaaa
    19981 taaaacattt ggacaaggag atgtaaaata atgacagtta aaaatttaaa aggtgtaaca
    20041 gctgcaagcg accaattgat gaaagctttt aaagatggta acgaagaatc ttttagcgca
    20101 gctatggtaa gcttatctaa ggaaattcag gataaaattt tagaagaagc aacagcaaaa
    20161 aatcaagatc aattagtatt aatgaaccgt ggtcagcgtg tattaactac acaagaaaca
    20221 aaattctata acgaagtagt gaaaaacgaa ggttttgcag gggtcgaaga attagtgcca
    20281 gctactgtat ttgaacgtgt atttgaagat ttagaacaat ctcatccact attgcaaaaa
    20341 attacttttg ttaacacaac tggtgtaaca gaatggattg tgtcacgtgg agtcaatcca
    20401 gcatggtggg gtaaactttg cgaagctgtt aaaaaagttt tagataatgg ctttgatgta
    20461 attaacatga agcagttcaa gctatcaggt tatattcctg tatgtaaggc aatgcttgat
    20521 ttaggtccag tatggttaga tcgttatgtc cgtactgttt tagtagaatc attgagaatt
    20581 gcattagaac aagcaattgt tgatggtact ggtaaagata tgccagtcgg aatgatgcgt
    20641 gacatgagca aacaaactag cggagaatat gctgaaaaaa aagcagaacc tattacagct
    20701 ttagatgctg taactatggg cggtttgatg gcgcgactat caaaattcaa tatcgaaggt
    20761 gtgaatgatc cgatttatcg taatgtgaat ccttctgatg tggtcctaat tgtgaatcca
    20821 actgattact ggtctaaagt attcccagct aagactgtac taactgctaa tggagaatac
    20881 gtacaagtat tgccagtacc agtttcagat ttgcagtcaa cggctgtgcc agaaggaaaa
    20941 gcagttattg gggtagcctc agattacttc atgggtgtag gatctacact aaaaattgaa
    21001 gcttcagatg aataccattt tgttgaagac gaacgcattt atctagctaa acaatatgca
    21061 aacggtcaac ctaaacgtaa cgatagtttc attgtattag atattagcgc tttgggaact
    21121 actactacaa ctacaaaacc aacaaccaca acaactacaa cacaagcgta ggtgatcaga
    21181 atgaagtata ttctttgtca gccggcaatc aatcggttta aatgggagct tgaagtttgt
    21241 ttaactaatc tgaagaaact aggaatcaaa gatatcgtat tgcttttcag cagacacgat
    21301 gatcagattc ctattttttt tgagaaggaa tatggtgttg aagttcatgt gtacgatgat
    21361 ctgcgggacg acaaagagta tattccttcg attaaaccat atttatggtg gaaatattta
    21421 gaagaagatc attcgcgtga ggacgaccga tatttctata tcgattcgga tgtcattttc
    21481 aataaaagaa ttaatttgcg caaattgcct tctaaagatg atgtttggta ttgtagcgac
    21541 tgctgtagtt atctaagtct tgattatatt agaagctgtg aaaacggaga aaatattcta
    21601 aaagatatgg caaacattgt aaatgttaca gtagaatctt tggaaactat aaacactaat
    21661 tcaggaggcg cacagtgggt tattaaccgt cctaaagcga attattggaa aaaggtttat
    21721 ctggattcta atcggctata tcgctacctt agagggcaaa aaacaaatat acaaatctgg
    21781 acagccgaga tgtgggcaca gctttggaac atgatgtatt tcaatattgg tcctaaagtt
    21841 cacgaggaat tagacttttg ttttgctact gatccaatag aaaaagttaa agaagtaaaa
    21901 atcttacaca atgctggagt aacaacaaac gatgaagatt tatttttcaa agggagatac
    21961 gtgacttcta cgccttttga tgaagattta tcatttgtaa acaagaaaaa atgctcttac
    22021 gcatatgtta aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg actgaagaat
    22081 tgctaatagc tgtgaaggat aatatttacg ttacctggaa cgaagaagat gagtcaatta
    22141 aaaagatgat agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca acactttctt
    22201 tttctcctga aagcttagaa tacggattgc taatcgaaag atgtagatac gactggaatc
    22261 gtgctttaga tgagtttgaa caaaatttcg ctagtgagtt attaggtttc attcaacatt
    22321 atgcgctaca agaatatatt gcaggtgatg tgaatggcga ataatcgtag actcgaagaa
    22381 acattcaacg atggttggtt aaagattttg acgcaaacta ccaaaagaaa tgaactagga
    22441 aaaaagattg gtgtagaaga tacagaaatc acttctttaa aatttagaaa tctttccatg
    22501 agagatagtg atataacagc tatggatgcg atgggatcga aattaactaa gaaagtaaag
    22561 actccatttc atccaatcgc caagaaattt aataaagatc aatattttat cgtaatcgat
    22621 agtatgcgtt acaacgttat ctatgccgat tacgataatt tttatatcta tttttatctt
    22681 gaaagtgtgg gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt taaataagca
    22741 aatttctgct atcaaaactt ccttagaaga gcattttggc ctcaaactct ttcaagactc
    22801 cgtaggcgag gatgagctac ctgatgattt taattacttc attctcgaaa caggagaaat
    22861 agaaatgatc actgagccaa aatatagcgt gggtcaaaat ctatatctaa ctttctattc
    22921 agaaaataga gaagatttaa caggagactc actagatatt atttcattga ttcaaaatcg
    22981 ttcgattcgt tttcagagaa tggatcccaa tcatttaaaa ctagagaacc aagatcgcta
    23041 tatcgatcaa ttggtattta cgtttagacg attattgaag agtgattgtc atggctaaaa
    23101 atagttggga gctaaaaata aatggacatg atgaacttct tgtgcggatg gaacgctatt
    23161 caagcgagag cgaacgactg attaacgaag cattgaaatc aaaaggttcg gctattgcag
    23221 tggataggat tacagaaaaa attcctgttt ctgaagcaga tttaagaaga gggcaccaac
    23281 acgcaaaaaa tagtcgtcca cttaagactc aatacattaa tttgggtttc atcattagac
    23341 ctacaagaaa atttgagtat ttaaaatatc ctgatttggg gataggtact tctaaaagaa
    23401 atcagccgga cgaatttatg agaagaggat taggtcttgc acttgatcca attacagaac
    23461 ttctgattcg tcaattcgat aaattaaata aataggggga acaacaatgg ctaaaacaaa
    23521 aactgtagta acaacgttcg ataacgtaag tatcaaacga attgctttta attttaagaa
    23581 cgcaggaaat gcaatcgcaa cgaattgtaa tggacaatta gatggcgaaa cagaaatgca
    23641 aacggtggtt aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac caatcaatat
    23701 gacggtaaca attactgcac atgtaccgat ggaagtttat cgacgtttca atgggttgaa
    23761 acaagatgga cgtattaaac caggcattta ctcttacggt cctgattccg taggcgaaga
    23821 tttctcactt gctgcagaga tcgtggatga cttcgaagaa aatagaaagt tagttggtat
    23881 gttagcatgc acttcgaata caggattaac attctctatt gaaaatggtg cggatgaagt
    23941 agctgcgtta gaactagaaa caaaagttat gcaagatgaa tttggtaaat tttatcatga
    24001 agcaattgtt gcagaacttg aagaagactt aacagatcaa tggatgacga atctatctgc
    24061 tgatgtgatt aaaaaaactt cagttgtgac aactacggcc actcaatcac agtaaaaaaa
    24121 acggaggtag cgaaatgaac gaagattact caaaaattga actaaacgat ggaacaattt
    24181 tgaatttaga acctaaactg aatatcaaga aattattgat gatcaataga gattttaaca
    24241 cagacgagtt tgcaaaaatg actgtgggaa aaggatccat ggatatttct gttattcaag
    24301 gtgcaaaggc tgtgtatatt gcttaccgcc aagcgaacat gactgattat atttcattcg
    24361 atgaatttat cgataaatgg gattttgata tggctactgc cagctatatt tatcaattga
    24421 tgatgttcaa acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca aataaggaaa
    24481 aaaagcttca aaagtaaaaa ttccaaagct cttagttgaa acgtgggtcg atgtctattc
    24541 gatgttgacc gacgtttttt ctatgccttc agatttggtt ttaagcgata tctgtttaga
    24601 tgacattttg caaatggctt acaacaagag tgcttatgaa ggatggaaaa actatgcaat
    24661 aaaccaatcc cagaaaaact aaagaaagga ggtaaaaaat ggctaaaaag agaacagaag
    24721 cagaagtaac tttcatagct aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg
    24781 ctgaattaac taaaaataga gcagaattaa aactagaaca agctcaatta caacagactg
    24841 gttctgaatc agacaagtta ggaagtaaat tatcttcttt agaaaagcag tatgaattac
    24901 aaagtcaaaa agttgaagta actagccaac gtttagccaa tgcgaaaaaa tattatggag
    24961 aaaattccac cgaagttcag aaacttgaaa gagaactgat taatcaacaa acagcgcaac
    25021 aacgtttgtc aaacgaaatt gataaaacga gtaatgcact agctcaagca aaaggcgaaa
    25081 tacagacgta cgagtctaca atgcaacagt tggatagcga acaaaaaaat gttcaagcta
    25141 gtgcttctct gattgaatcc gaatacaaaa aatggcaagc aactgctggt caatcagctt
    25201 ctgaagccga gaaattagcg aaagcccaag aatatgtttc tcaacaatct gaaaatgcgg
    25261 agaaaacgat agatatccta agacgacagt tagaagctac acagtctgaa tttggcgcta
    25321 catccacaga agcaatgcag atggaagcga agcttaatga tgctgaacgt gaatttgaag
    25381 agttaggaca agctgctaaa aatgtagata caactaactt ggacgatatc ggaagcaaaa
    25441 tagatatgaa taatttaatg gaagcttctg acgttttaag cgacattggc gataagctta
    25501 cagaattagg gaaacaagca gtggactctg ctaacagtgt aggtagttcc cagagtaaga
    25561 tacaagctaa ttttggtttg tctaaacaag aggctgaaga attaacgaat gtagccagag
    25621 acatttatta taaaggtttt ggagaatcgt tagatcagtc cacagatgca ttgattttgg
    25681 taaagcgtaa tttaggagat ttaaataatc aagatttaca aaatatcacg gaacaagcta
    25741 tggtcctaga aaacaccatg ggcgctgata tggatgaaac gttacgtggt gtaaatggct
    25801 taatggtcaa tttcggcttg agtgctcaag atgcaatgga tttaatggtt tcgggtactc
    25861 aaaacggttt agataaaacg cacgaattag gcgacaatat ggcagaatat agccaattat
    25921 ggagtcaaat gggatattca gctgatgaaa cgttcggaat gcttcaaaat ggtttagatg
    25981 cgggtgctta taaccttgat aaagtcaatg acttagttaa ggaaatggga atatcgttaa
    26041 cagatggtcg atttgagcaa aacatggata tgtttagtga aagtactaga aaagcttttg
    26101 aagagtggaa aaatggcgga ggaacacaaa aagacgttat taattccatg attcaagatt
    26161 ttagcaatat ggatggtcaa tacgaccaat taaataaagc ttcgacaatt tggtctgcgc
    26221 ttggcgaaga taatgcgatg aaagttgtcc aatctttaac tgatgttaac catacatttg
    26281 atgatgttag tggatctgca caaaaaatga atgaagattc tactactccg ttgcaagagt
    26341 tgaacgggaa aatagctgaa ttaaaggatt cattagctcc tataggcaac acaatcatag
    26401 atgcactcga accagtaatt gattttctag gaaagatggc tgatgcgttt aataatcttc
    26461 cacaaccagt acaggatttc attgttgcta taggtggtct tacagcagca tttggcatac
    26521 tgttaccggc aatactagcc gtgtcatatt tatttggtcc gatgatgctt ataattggag
    26581 gaattatagc tgttatagca ggcgttattg tggcaattaa gaactggggt gcaattactg
    26641 actggtttag tggtttatgg aaaaaattta ctgattggtt gggtgatact tgggaaagta
    26701 taaaagacgg agcctcatca gtttgggatg gggttaaaga aacctggtct ggatttgtag
    26761 attgggttca agatatttgg caaggagttt ctgattggtt tggagagcta tggagcggat
    26821 tagttgaagg agcttccaac atctggcaag gagtccaaga aacttggcaa acattcgttg
    26881 attgggtttc aaatatttgg aacggagtca aagaagtatg gtcgattatt tgggcagaca
    26941 ttgtaggaat tgttcaaata ccatggacat taataacgtc attgattcaa gctggtatta
    27001 atattatcgt gggtattttt gatgtagctg gacagttatt gggcgcagct tggcaagctg
    27061 tttggacacc tatttctgat ttccttaaaa atacttggga tactatgaca caatggataa
    27121 gtatcgcttg gaatgggatt gtaactacat tccatactat atttgatcca gtagtggcat
    27181 ggtggaatgg tatatggaca gctattagta ctacggcttc aaatatttgg aattcaatta
    27241 gtgcaacagc ttctagtatt tggaacagta tcaagaatac aatcactagc ttggtacaag
    27301 cagctgctac agtaattcaa aatatttggt caactgtatc tagttggtta ggtggaattt
    27361 ggaattcaat cagctctaca gcatcaaata tctggaatag tgtgactagt agtataagca
    27421 atgctataaa cgcagctaaa agtgccattc aaagtgtttg gaatagtata tcttcgtgga
    27481 ttagcggaat ttggaacggt atcaaaaata ctgctttgaa tctttggaat ggaattacaa
    27541 gcactattag ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc
    27601 taacaggtat tgtttccgac atattcaata atgttaaaag tacaattgct aacatatggg
    27661 aaggcatcaa aaagactgtt agcgctccaa ttgattggat tagagacaaa atcagtggca
    27721 tttttgataa tttgaatatt tctataccac atattccgtt accggaattt atcatggaag
    27781 gcagctttaa cccgctaaaa ggtcaaatcc cccatttgcg tgtaaaatgg catgctaaag
    27841 gaggtatctt tactaaacca actttactag gtggaatgaa cggtgtcggt gaagcaggac
    27901 ctgaagcagt tttacctttg aaaagatctg ttttgcaaga aattggtgat cgtatcttga
    27961 gtagcacatc agtttcatct agggcacaaa cgattcaacc tgtgaataac tacgaattca
    28021 atttcacaat tgatggtaac gcagatgagg ttactatgaa gcaaacaact caacaaatca
    28081 ttgatagcat tacaaaaatt caaaatgata atgcttcggc atggcgttaa acaggagagt
    28141 atttctcctg tttttttagt attaaaaagg atgtgaaaaa atgactgatt gtatacattc
    28201 tataatcgat ggatttcctg attatttgca taaattggct ttagctgaaa gaccaaccat
    28261 accttctcca aaaagacaga gagttgaaac ttctgtttta ggtaggttag gtggcttagt
    28321 acaagattac tcgtttgaag atatgtcgtt tacattgcac tataactatt tagaggatgt
    28381 ggaagaccat caagcgttca agcaatcgtt ttatatcatg cgtcattggt taaactatgc
    28441 aaagaaatta gaattctctg acgatcccaa cgtctattac gttatccaga ctatcgatat
    28501 tggggatgca gaaaacgata ttgttgaatg gggagagttc gatgtaaata ttactgcgaa
    28561 accattcgca agagttcaag aagatgtacc tataaccgta gataaaccac agtcatttaa
    28621 cttgctgaat aatagtttag aagaaagttt tccaaagatt atcatcactc cttcagctac
    28681 ttcatgccag ttcatcttaa atgattatgt gtttagtttc gaaggcttag tagcaggaac
    28741 tgatgtagtc attgatagtg atttgatgct ttgctacgaa gagcaatcgg acggagatat
    28801 tttagatcgg tccaacaaaa tgaagaccat gcaatatccg acattgcaag tggatatcaa
    28861 tcattttaat tgcactggtt tgagcaaaat acaaatttat cgtaacgggt taaggtaggt
    28921 gaaatagatg atcgataatt taataactat ttacgataaa aatgacgcga ataatttagc
    28981 tgaacattta tatgatacgc aaggtttagg cgctttgtca gactggttaa cagctactgt
    29041 tagcaataaa ctaaacggag ccgagatatt tcagggtact tattcaataa acggaactaa
    29101 tgcagatttg attgtagacg gacgtattat tcagtgttat gtagatgaaa atcgagcaaa
    29161 acagcgtcta cggatttatt atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa
    29221 agctgaacct attttcaatg atataagaaa atcggtgttg aataaatatg acagcggaac
    29281 agaaaagatc actgctactc aggcatggca aaacgcaaaa gttttagcga aaccagctat
    29341 cccttcgcag ttttctttct cgtcattagt agatacgctt gctaatgtga agatagaaaa
    29401 ggcgaatttt ttagaattct ttggtggaaa agagggatct attctagatc gatttcatgg
    29461 tgagtttcta aaagataata acacattacg tcatgaaaaa agtctaggta cggatcataa
    29521 aatcaaagcg atttatacta aaaacttaac tggtcttgac ttagagattg atgctcaaag
    29581 tgttttagtt ggagtttatc cattcattag cagctcttca gaaggagaag acgagatcac
    29641 tctaccagaa gaagttattt tcacggatta cgtggatgat tatcctgctg gatatgtttc
    29701 ttttgttgat tttaaagaca aagcgactga tgtagccaca ttaagggaag ctgctaaaga
    29761 ctggttgaaa acaaacatag ataaacaaaa accacaagtg agtggttcga ttgaattagt
    29821 accattgagg catcaaagag gctatgaaaa atttgttgat ctagaaaaag tttcgatggg
    29881 tgacggagta gatgtgtatc atccacagtt aaaagtgaat atgtcagcga gaattgtgga
    29941 atatacgttt aatgttctaa ccaattcata cgataaatta gttgtaggaa acgtcaaaac
    30001 aaacttctta gaaaacacag agaataatgt cagcaatttg attaatgatg ccattgatca
    30061 attgaaaaac ggtggcgaaa tcagtgattt actcaatgat attgtagatc atcaaactga
    30121 tatgattact ggtcaaaatg gtggttatgt tttattagat cctaaagaag cgcctagtcg
    30181 tattttgatt atggacacac cagataagaa taccgcaagg aatgttttac aaatcaacaa
    30241 tgctggtatt ggtttttcta aaactggcat taatggaaca tatgaaacgg catggacgtt
    30301 agatggcgga ttcaatgcct cgtttattac ggctggtgaa atagtaggaa ttactattag
    30361 aggtactaca ttaattagtg atggtgctga ttatagaaca agtattgcta atggcaaaat
    30421 gacttggtac tcaaaaaaag ttaacaaaga tattatggag ctagaagcac gtgattatgt
    30481 aagtgctgat gccggtattg tatcatacac catgaaaact ggtggtggtt tcatgattag
    30541 aaatccacag ggtaacttgg tttttagtac gtgggataat ggtaataaca gaccgtttct
    30601 atcttttggt gcgcccaatt tcaggtatag caatgctagt tatgtaactt ctggcgacgg
    30661 tagttcttta agcattaatg gtagtgcggg taactcatgg gaatttaagg tagctggtag
    30721 gactatgaaa tttactagtg atggtatgct aacgttacca ggttgttttt ttggttcatg
    30781 ggaagatggg aaacttgcta ggtttgaaca atcaacggta caagtatata aagattttac
    30841 tgttagaggt actaaaaact caactgtacc aacagaacat tatggacaac gactattgaa
    30901 cgcttatgaa actccagaat attatttcgc tgattatggg gaagccgtta caggtgacaa
    30961 tggtaaagtt cgtgttgata ttgaccccat gtttgctgaa acagtaaatc taagtcggta
    31021 tatgacacat gtgacaccta cagaactagt tttgtgtgct gttactcatg aagatattga
    31081 ccatttcatc attgaaacta gtaagccaaa cgtattagtt agatggaatt tagtggcaca
    31141 ccgtctaggg tatgaagata ttagattaaa agaggataca gcatatgata gcacagtgct
    31201 tgaccaaaaa cgtttttaaa acgaagacaa ggaggtatat aaatggctag cagtttatat
    31261 aatttggctt tagatttcag caaagaatta aactacacca aagctattat ggctcgtcaa
    31321 ggtgataaag ggattacggt gacggttaaa ccgtttctaa atggcttgca gatggatacg
    31381 agtggcggaa catttacttt aaaaggaaca acaccatcta accgttacgt agataatgtt
    31441 gcaactagtg taactagtga agaagtcacg ttttctcttg atggcacatt tatgagtgaa
    31501 gcaggatatt ataaacactg ctacgtagaa tatagaaaag acaatcaaat tttaacaacg
    31561 caagatatca tttttttctc actaggagtg tctgacattt cgcaaggcca agccgatgaa
    31621 tatgtttcgc aattagaaga gttgattcga aagtacaacg aaacttttga tgcttttatg
    31681 gctgaaatca aaggtagagt ggatagctta aatcaacaga ttactgattt aactggtcaa
    31741 gctaaaacgc tacaagacaa gttagatgct ctgaaagaag aaatttctaa gttaggtaac
    31801 ttacaagtga tgtacagtaa cagcatcgac ttcggggact atgattattc ggagaacccg
    31861 aatttaatgc catatattac agaaccttgg gttggacctt tgttaggtaa tggacataca
    31921 gttaaagata gtgttaaaag ggttataact catactaaaa ctagaacagc taattccggc
    31981 gacatattat cgcttggctt aggtattccg tgtacagcgg aagctaataa taggtatctt
    32041 ataaccactt tacgaccatc tacgacatac acattaagcg ttactatgag tgtcggttca
    32101 gattggactg gagagaccaa tactataggg gttaggctta gatatttaaa tgaacaaggt
    32161 ggtatagaac tgcccattaa tgcattaata ccagcaaatg tagaacgcga taaaatggtc
    32221 actcatacat ttactggtat aacaaaagat aatgtaacta gcataacaaa ctgttatgtt
    32281 gaaatattct cacttaatag tgaatataaa ggaacggtta gtgtcagcta tgacgttaag
    32341 ttaaaagcac actaccctaa cttactagat ggcccttact ggttaggtaa agttccttta
    32401 ggcgagaata ttgctgaccc tacagttgtc tttccacaca aaactagtga atatatggtt
    32461 tatggtagac gcaatacaga aaactatatc gctgaccaaa catataccat atcaatgaaa
    32521 gcaactaaac taacagttca atcgtttgcc gtgtatattg ctgctggccg cgttaaagta
    32581 ggagacatga agcctactga ggggttagct aatacatggg agttaacttt tactgtaact
    32641 aagcaacata tagatagtgg ggtaactaat tatctggaaa tataccaata cccgtcagct
    32701 acaaaaggag ctgttcaaat tgagtggctc aaactagaaa aaggcaatac tcgaaccccg
    32761 aatattagtg agtataaata tcgtggtact ggtatgcgtg attcaaacaa tccaaaagat
    32821 tatgtttggg atctagcacc agaatatgtc gaagataatt tggccacaga tattaaaatt
    32881 tctgaaatta ctggtaaagc aaacaattat accgatggga aagtatcgga gattaattcg
    32941 cagttgactg cttcaattaa tgaagtagac accacagcta aggatgctca aacaaaagcg
    33001 aatgctaatg cgactgctat agatgaatta gacaataaga tcgatgaacg cattaatgat
    33061 acagctacta ccacattaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
    33121 gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagcag tggtggaaat
    33181 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
    33241 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
    33301 gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac
    33361 acgatttaga aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg
    33421 attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga
    33481 accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac
    33541 atttggaaat atggacgtac tggcggagag tacgcaggaa aagtattgga cgacatgctt
    33601 gtatccgttc cttacacaga tcagccaccg cttgaaggaa ttcgtgctga tggcgaaccg
    33661 ttaacaatcg ctgatcaaat gtttgatcct aaattgaatc aatggattat tttagcgaac
    33721 gcactagatc acaacgattt aaacaatctc aaagcaatgt atgagtcgtt agaaaatgag
    33781 aacggcgatt taaaacagat caatgccaaa ctcatgctaa gcgatgtagc aattaaacag
    33841 gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc aaaaatgatg
    33901 cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct
    33961 tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg
    34021 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaaaaagc
    34081 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
    34141 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg
    34201 ctatcgtttc tttgatttta ttagtaataa aaccaattac tgcatctttc tcgaagatta
    34261 ctgagactct ttcaaaagta agtcacaatt tagatttgct gactaaagat ttagaatcga
    34321 gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata
    34381 cacatgcaga aaaattggta gaacacacgc aacaaattaa aactttattt agggagagaa
    34441 gaaaatgaat aataaaacgt tcgaagtact aaaatggttc gcactggtaa ttattcccgc
    34501 actagctact ttcgtggggt tagttggtaa agcgctcaat tggcagtaca cagatatctg
    34561 tgttgtcatc attactggtt ttggcgcgtt tttagggagt gtgttgggtg tatcaaatcg
    34621 aacctacaaa atgttctcgg ctgaaagcga agaaggagga aacaaatgaa aaagaaaatt
    34681 actattactg cgatgagcct gttaacggct ctttttttat tacccattaa tacgtttgct
    34741 tatactatta atgacgagta taatttagcg ccgaatcaag gagactccag attagcaatt
    34801 cctaacaaaa ttattttgca cgaaactgga atagatgcac cagcaagaaa cgtagccgcc
    34861 aacatgaaaa ataattataa cggaagcaat tcttatacta cagatgttat tggtgacggt
    34921 gggattgttt accgtgtggg tgagcaagga tatgtttcgt ggggagctgg taacgctaat
    34981 ccttatgcgc ctgtacagat tgaattacag cgcacatatg ataaagcatt gtttgaaaaa
    35041 aactatcgag cttatattga atatacaaga gatagtgcaa aaaaatatgg aattccattg
    35101 actcttgatc aaggaacttc tttatttaca aaaggaatca tttctcattt gtgggtgaca
    35161 aattatgttt gggggaacca cacagatcca tatggttact tatcgcaaat gggagttagc
    35221 aaagaaaagc ttgcttatga tttagctcat ggatttaccg atgaaaatcc aacaacttct
    35281 gaaaacaagc ctgtcattga tccaacacga gctggtgcag ctaatcctac gctgacagat
    35341 ggaacaaatc acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa cttgcatgtg
    35401 gctggatggc acattgctaa ctataaatac gagtatattt tcattatgga ctacaatact
    35461 gggaaagaat tagctcgagt aagagctgat ggaatttata gaccagatgt aaaccaagct
    35521 tataatactt ctggaaatgt tggttatcat gtatctttca atatgcgtaa ttttcctagt
    35581 aagaaagtct atgtaatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt
    35641 gcgcaagatt tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc
    35701 ctcgttgagg ag
//
LOCUS       DBNIBO010000010        34420 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.010, whole genome shotgun sequence.
ACCESSION   DBNIBO010000010 DBNIBO010000000
VERSION     DBNIBO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.010"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(73..1965)
                     /locus_tag="AC1Y77_000479"
     CDS             complement(73..1965)
                     /locus_tag="AC1Y77_000479"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574085.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1962..3689)
                     /locus_tag="AC1Y77_000480"
     CDS             complement(1962..3689)
                     /locus_tag="AC1Y77_000480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574086.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3705..4355)
                     /locus_tag="AC1Y77_000481"
     CDS             complement(3705..4355)
                     /locus_tag="AC1Y77_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1574087.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4559..4855
                     /locus_tag="AC1Y77_000482"
     CDS             4559..4855
                     /locus_tag="AC1Y77_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIQ1574088.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5047..5667
                     /locus_tag="AC1Y77_000483"
     CDS             5047..5667
                     /locus_tag="AC1Y77_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIQ1574089.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5759..6925)
                     /locus_tag="AC1Y77_000484"
     CDS             complement(5759..6925)
                     /locus_tag="AC1Y77_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1574090.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6937..7290)
                     /locus_tag="AC1Y77_000485"
     CDS             complement(6937..7290)
                     /locus_tag="AC1Y77_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HIQ1574091.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7418..8440
                     /locus_tag="AC1Y77_000486"
     CDS             7418..8440
                     /locus_tag="AC1Y77_000486"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIQ1574092.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8459..8620
                     /locus_tag="AC1Y77_000487"
     CDS             8459..8620
                     /locus_tag="AC1Y77_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574093.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8879..9313)
                     /locus_tag="AC1Y77_000488"
     CDS             complement(8879..9313)
                     /locus_tag="AC1Y77_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574094.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9423..11282)
                     /locus_tag="AC1Y77_000489"
     CDS             complement(9423..11282)
                     /locus_tag="AC1Y77_000489"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIQ1574095.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11283..11423
                     /locus_tag="AC1Y77_000490"
     CDS             11283..11423
                     /locus_tag="AC1Y77_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HIQ1574096.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11400..12559)
                     /locus_tag="AC1Y77_000491"
                     /pseudo
     CDS             complement(11400..12559)
                     /locus_tag="AC1Y77_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(12866..14290)
                     /gene="araA"
                     /locus_tag="AC1Y77_000492"
     CDS             complement(12866..14290)
                     /gene="araA"
                     /locus_tag="AC1Y77_000492"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIQ1574097.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14306..15004)
                     /locus_tag="AC1Y77_000493"
     CDS             complement(14306..15004)
                     /locus_tag="AC1Y77_000493"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIQ1574098.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14961..16622)
                     /locus_tag="AC1Y77_000494"
     CDS             complement(14961..16622)
                     /locus_tag="AC1Y77_000494"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIQ1574099.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16840..18240
                     /locus_tag="AC1Y77_000495"
     CDS             16840..18240
                     /locus_tag="AC1Y77_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HIQ1574100.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18492..19571)
                     /locus_tag="AC1Y77_000496"
     CDS             complement(18492..19571)
                     /locus_tag="AC1Y77_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1574101.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19657..20616)
                     /locus_tag="AC1Y77_000497"
     CDS             complement(19657..20616)
                     /locus_tag="AC1Y77_000497"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIQ1574102.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20628..22139)
                     /locus_tag="AC1Y77_000498"
     CDS             complement(20628..22139)
                     /locus_tag="AC1Y77_000498"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIQ1574103.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22161..22724)
                     /locus_tag="AC1Y77_000499"
     CDS             complement(22161..22724)
                     /locus_tag="AC1Y77_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIQ1574104.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22730..23593)
                     /locus_tag="AC1Y77_000500"
     CDS             complement(22730..23593)
                     /locus_tag="AC1Y77_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574105.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23607..24503)
                     /locus_tag="AC1Y77_000501"
     CDS             complement(23607..24503)
                     /locus_tag="AC1Y77_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574106.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24678..25967)
                     /locus_tag="AC1Y77_000502"
     CDS             complement(24678..25967)
                     /locus_tag="AC1Y77_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574107.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25939..27456)
                     /locus_tag="AC1Y77_000503"
     CDS             complement(25939..27456)
                     /locus_tag="AC1Y77_000503"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIQ1574108.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27654..28544
                     /locus_tag="AC1Y77_000504"
     CDS             27654..28544
                     /locus_tag="AC1Y77_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1574109.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28617..29441)
                     /gene="nadE"
                     /locus_tag="AC1Y77_000505"
     CDS             complement(28617..29441)
                     /gene="nadE"
                     /locus_tag="AC1Y77_000505"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HIQ1574110.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29453..30925)
                     /locus_tag="AC1Y77_000506"
     CDS             complement(29453..30925)
                     /locus_tag="AC1Y77_000506"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIQ1574111.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31562..31924)
                     /locus_tag="AC1Y77_000507"
     CDS             complement(31562..31924)
                     /locus_tag="AC1Y77_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HIQ1574112.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32147..32899
                     /locus_tag="AC1Y77_000508"
     CDS             32147..32899
                     /locus_tag="AC1Y77_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1574113.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33039..33962)
                     /locus_tag="AC1Y77_000509"
     CDS             complement(33039..33962)
                     /locus_tag="AC1Y77_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574114.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33977..34249)
                     /locus_tag="AC1Y77_000510"
     CDS             complement(33977..34249)
                     /locus_tag="AC1Y77_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574115.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10156 a         7169 c         5920 g        11175 t
ORIGIN      
        1 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
       61 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      121 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      181 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      241 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      301 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      361 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      421 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      481 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      541 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      601 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      661 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      721 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
      781 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
      841 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
      901 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
      961 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1021 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1081 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1141 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1201 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1261 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1321 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1381 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1441 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1501 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1561 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1621 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1681 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1741 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     1801 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     1861 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     1921 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     1981 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2041 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2101 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2161 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2221 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2281 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2341 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2401 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2461 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2521 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2581 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2641 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2701 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     2761 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     2821 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     2881 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     2941 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3001 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3061 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3121 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3181 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3241 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3301 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3361 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3421 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3481 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3541 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3601 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3661 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3721 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     3781 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     3841 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     3901 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     3961 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4021 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4081 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4141 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4201 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4261 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4321 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4381 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4441 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4501 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4561 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4621 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4681 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4741 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     4801 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     4861 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     4921 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     4981 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5041 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5101 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5161 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5221 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5281 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5341 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5401 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5461 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5521 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5581 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5641 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5701 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     5761 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     5821 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     5881 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     5941 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6001 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6061 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6121 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6181 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6241 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6301 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6361 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6421 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6481 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6541 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6601 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6661 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6721 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     6781 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     6841 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     6901 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     6961 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7021 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7081 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7141 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7201 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7261 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7321 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7381 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7441 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7501 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7561 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7621 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7681 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7741 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     7801 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     7861 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     7921 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     7981 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8041 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8101 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8161 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8221 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8281 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8341 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8401 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8461 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8521 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8581 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8641 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8701 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     8761 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     8821 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     8881 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     8941 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9001 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9061 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9121 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9181 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9241 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9301 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9361 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9421 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9481 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9541 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9601 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9661 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9721 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
     9781 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
     9841 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
     9901 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
     9961 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10021 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10081 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10141 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10201 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10261 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10321 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10381 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10441 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10501 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10561 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10621 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10681 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10741 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    10801 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    10861 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    10921 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    10981 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11041 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11101 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11161 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11221 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11281 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11341 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11401 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11461 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11521 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11581 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11641 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11701 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    11761 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    11821 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    11881 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    11941 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12001 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12061 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12121 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12181 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12241 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12301 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12361 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12421 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12481 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12541 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12601 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12661 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12721 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    12781 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    12841 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    12901 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    12961 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13021 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13081 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13141 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13201 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13261 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13321 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13381 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13441 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13501 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13561 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13621 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13681 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13741 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    13801 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    13861 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    13921 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    13981 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14041 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14101 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14161 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14221 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14281 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14341 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14401 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14461 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14521 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14581 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14641 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14701 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    14761 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    14821 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    14881 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    14941 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15001 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15061 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15121 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15181 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15241 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15301 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15361 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15421 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15481 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15541 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15601 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15661 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15721 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    15781 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    15841 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    15901 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    15961 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16021 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16081 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16141 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16201 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16261 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16321 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16381 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16441 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16501 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16561 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16621 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16681 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16741 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    16801 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    16861 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    16921 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    16981 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17041 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17101 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17161 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17221 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17281 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17341 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17401 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17461 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17521 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17581 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17641 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17701 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    17761 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    17821 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    17881 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    17941 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18001 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18061 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18121 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18181 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18241 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18301 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18361 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18421 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18481 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18541 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18601 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18661 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18721 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    18781 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    18841 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    18901 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    18961 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19021 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19081 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19141 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19201 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19261 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19321 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19381 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19441 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19501 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19561 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19621 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19681 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19741 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaagt
    19801 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    19861 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    19921 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    19981 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20041 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20101 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20161 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20221 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20281 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20341 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20401 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20461 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20521 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20581 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20641 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20701 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    20761 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    20821 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    20881 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    20941 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21001 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21061 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21121 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21181 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21241 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21301 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21361 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21421 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21481 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21541 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21601 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21661 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21721 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    21781 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    21841 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    21901 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    21961 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22021 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22081 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22141 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22201 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22261 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22321 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22381 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22441 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22501 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22561 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22621 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22681 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22741 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    22801 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    22861 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    22921 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    22981 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23041 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23101 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23161 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23221 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23281 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23341 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23401 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23461 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23521 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23581 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23641 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23701 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    23761 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    23821 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    23881 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    23941 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24001 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24061 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24121 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24181 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24241 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24301 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24361 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24421 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24481 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24541 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24601 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24661 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24721 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    24781 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    24841 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    24901 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    24961 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25021 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25081 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25141 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25201 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25261 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25321 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25381 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25441 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25501 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25561 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25621 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25681 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25741 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    25801 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    25861 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    25921 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    25981 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26041 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26101 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26161 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26221 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26281 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26341 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26401 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26461 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26521 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26581 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26641 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26701 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    26761 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    26821 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    26881 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    26941 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27001 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27061 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27121 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27181 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27241 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27301 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27361 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27421 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27481 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27541 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27601 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27661 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27721 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    27781 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    27841 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    27901 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    27961 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28021 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28081 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28141 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28201 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28261 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28321 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28381 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28441 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28501 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28561 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28621 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28681 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28741 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    28801 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    28861 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    28921 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    28981 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29041 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29101 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29161 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29221 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29281 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29341 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29401 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29461 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29521 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29581 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29641 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29701 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    29761 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    29821 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    29881 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    29941 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30001 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30061 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30121 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30181 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30241 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30301 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30361 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30421 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30481 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30541 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30601 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30661 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30721 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    30781 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    30841 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    30901 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    30961 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31021 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31081 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31141 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31201 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31261 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31321 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31381 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31441 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31501 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31561 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31621 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31681 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31741 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    31801 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    31861 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    31921 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    31981 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32041 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32101 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32161 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32221 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32281 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32341 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32401 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32461 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32521 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32581 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32641 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32701 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    32761 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    32821 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    32881 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    32941 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33001 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33061 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33121 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33181 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33241 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33301 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33361 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33421 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33481 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33541 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33601 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33661 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33721 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    33781 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    33841 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    33901 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    33961 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34021 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34081 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34141 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34201 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34261 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34321 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34381 ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DBNIBO010000011        31544 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.011, whole genome shotgun sequence.
ACCESSION   DBNIBO010000011 DBNIBO010000000
VERSION     DBNIBO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.011"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(34..753)
                     /locus_tag="AC1Y77_000511"
     CDS             complement(34..753)
                     /locus_tag="AC1Y77_000511"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIQ1574116.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    943..990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            1090..1422
                     /locus_tag="AC1Y77_000512"
     CDS             1090..1422
                     /locus_tag="AC1Y77_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574117.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1409..3412
                     /locus_tag="AC1Y77_000513"
     CDS             1409..3412
                     /locus_tag="AC1Y77_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIQ1574118.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3431..3901
                     /locus_tag="AC1Y77_000514"
     CDS             3431..3901
                     /locus_tag="AC1Y77_000514"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HIQ1574119.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3922..4506
                     /locus_tag="AC1Y77_000515"
     CDS             3922..4506
                     /locus_tag="AC1Y77_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIQ1574120.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4521..5507
                     /locus_tag="AC1Y77_000516"
     CDS             4521..5507
                     /locus_tag="AC1Y77_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIQ1574121.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            5497..5808
                     /locus_tag="AC1Y77_000517"
     CDS             5497..5808
                     /locus_tag="AC1Y77_000517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIQ1574122.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5830..7611
                     /locus_tag="AC1Y77_000518"
     CDS             5830..7611
                     /locus_tag="AC1Y77_000518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIQ1574123.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7604..8980
                     /locus_tag="AC1Y77_000519"
     CDS             7604..8980
                     /locus_tag="AC1Y77_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIQ1574124.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            8987..9622
                     /locus_tag="AC1Y77_000520"
     CDS             8987..9622
                     /locus_tag="AC1Y77_000520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIQ1574125.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9652..11007
                     /locus_tag="AC1Y77_000521"
     CDS             9652..11007
                     /locus_tag="AC1Y77_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIQ1574126.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11199..12593)
                     /locus_tag="AC1Y77_000522"
     CDS             complement(11199..12593)
                     /locus_tag="AC1Y77_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIQ1574127.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12685..13656
                     /locus_tag="AC1Y77_000523"
     CDS             12685..13656
                     /locus_tag="AC1Y77_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIQ1574128.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13653..14435
                     /locus_tag="AC1Y77_000524"
     CDS             13653..14435
                     /locus_tag="AC1Y77_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIQ1574129.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(14604..14975)
                     /gene="gloA"
                     /locus_tag="AC1Y77_000525"
                     /gene_synonym="lguL"
     CDS             complement(14604..14975)
                     /gene="gloA"
                     /locus_tag="AC1Y77_000525"
                     /gene_synonym="lguL"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIQ1574130.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            15109..15552
                     /locus_tag="AC1Y77_000526"
     CDS             15109..15552
                     /locus_tag="AC1Y77_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIQ1574131.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15595..16404)
                     /gene="yidA"
                     /locus_tag="AC1Y77_000527"
     CDS             complement(15595..16404)
                     /gene="yidA"
                     /locus_tag="AC1Y77_000527"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HIQ1574132.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16423..17790)
                     /locus_tag="AC1Y77_000528"
     CDS             complement(16423..17790)
                     /locus_tag="AC1Y77_000528"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIQ1574133.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17774..18604)
                     /locus_tag="AC1Y77_000529"
     CDS             complement(17774..18604)
                     /locus_tag="AC1Y77_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIQ1574134.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18767..19204
                     /locus_tag="AC1Y77_000530"
     CDS             18767..19204
                     /locus_tag="AC1Y77_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HIQ1574135.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19280..19900
                     /gene="rpoE"
                     /locus_tag="AC1Y77_000531"
     CDS             19280..19900
                     /gene="rpoE"
                     /locus_tag="AC1Y77_000531"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HIQ1574136.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20089..20595
                     /locus_tag="AC1Y77_000532"
     CDS             20089..20595
                     /locus_tag="AC1Y77_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIQ1574137.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20612..21511
                     /locus_tag="AC1Y77_000533"
     CDS             20612..21511
                     /locus_tag="AC1Y77_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HIQ1574138.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            21598..22482
                     /locus_tag="AC1Y77_000534"
     CDS             21598..22482
                     /locus_tag="AC1Y77_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIQ1574139.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(22674..22745)
                     /locus_tag="AC1Y77_000535"
     tRNA            complement(22674..22745)
                     /locus_tag="AC1Y77_000535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22710..22712),aa:Arg,seq:ccg)
     gene            22805..24139
                     /gene="rpoN"
                     /locus_tag="AC1Y77_000536"
     CDS             22805..24139
                     /gene="rpoN"
                     /locus_tag="AC1Y77_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIQ1574140.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            24288..25379
                     /locus_tag="AC1Y77_000537"
     CDS             24288..25379
                     /locus_tag="AC1Y77_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIQ1574141.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            25420..26421
                     /gene="gap"
                     /locus_tag="AC1Y77_000538"
     CDS             25420..26421
                     /gene="gap"
                     /locus_tag="AC1Y77_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIQ1574142.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            26531..27721
                     /locus_tag="AC1Y77_000539"
     CDS             26531..27721
                     /locus_tag="AC1Y77_000539"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIQ1574143.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            27825..28580
                     /gene="tpiA"
                     /locus_tag="AC1Y77_000540"
     CDS             27825..28580
                     /gene="tpiA"
                     /locus_tag="AC1Y77_000540"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIQ1574144.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            28696..29994
                     /gene="eno"
                     /locus_tag="AC1Y77_000541"
     CDS             28696..29994
                     /gene="eno"
                     /locus_tag="AC1Y77_000541"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIQ1574145.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            30393..31532
                     /locus_tag="AC1Y77_000542"
     CDS             30393..31532
                     /locus_tag="AC1Y77_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIQ1574146.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        10396 a         5576 c         6755 g         8817 t
ORIGIN      
        1 aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa
       61 taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag
      121 gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt tggaaaaggc
      181 gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa
      241 gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt
      301 tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga
      361 aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac
      421 tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc
      481 tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact
      541 aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga
      601 atctggagac atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac
      661 aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag
      721 ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt
      781 gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct
      841 tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat
      901 attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca
      961 aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa
     1021 gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat
     1081 cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa
     1141 aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag
     1201 caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa
     1261 aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca
     1321 gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt
     1381 gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct
     1441 catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt
     1501 tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc
     1561 agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga
     1621 catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca
     1681 tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt
     1741 catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc taactgaaaa
     1801 acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc
     1861 tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa
     1921 ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga
     1981 ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat
     2041 agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc
     2101 taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa
     2161 gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga
     2221 agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta
     2281 tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt
     2341 aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga
     2401 gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt
     2461 gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac
     2521 accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat
     2581 gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt
     2641 cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc
     2701 atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct
     2761 atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca
     2821 gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag
     2881 tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat
     2941 tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat
     3001 tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc
     3061 tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta
     3121 tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct
     3181 agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt
     3241 acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca
     3301 gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa
     3361 aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga
     3421 tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt
     3481 agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac
     3541 aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat
     3601 tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt
     3661 tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct
     3721 gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg
     3781 tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat
     3841 ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta
     3901 agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa
     3961 gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac
     4021 tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc
     4081 gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa
     4141 gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa
     4201 atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta
     4261 cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag
     4321 acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact
     4381 gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc
     4441 agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc
     4501 gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag
     4561 agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag
     4621 cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat
     4681 atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat
     4741 cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga
     4801 aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg
     4861 gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc
     4921 ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc
     4981 ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg
     5041 ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata
     5101 tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac
     5161 tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg
     5221 aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc
     5281 gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat
     5341 tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg
     5401 caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg
     5461 aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt
     5521 ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca
     5581 accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt
     5641 tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa
     5701 atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa
     5761 gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag
     5821 atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag
     5881 aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga
     5941 gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga
     6001 attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca
     6061 gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc
     6121 acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa
     6181 tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg
     6241 atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga
     6301 acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa
     6361 accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg
     6421 ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat
     6481 acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg
     6541 aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg
     6601 ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt
     6661 gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac
     6721 atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc
     6781 cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca
     6841 cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat
     6901 cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca
     6961 gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt
     7021 tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc
     7081 agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat
     7141 tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc
     7201 accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta
     7261 gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt
     7321 gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa
     7381 caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg
     7441 ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga
     7501 cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc
     7561 aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg
     7621 tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata
     7681 tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga
     7741 agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg
     7801 tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat
     7861 cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga
     7921 aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga
     7981 gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa
     8041 attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg
     8101 acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat
     8161 cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg
     8221 ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg
     8281 tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat
     8341 catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga
     8401 agttccagga agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga
     8461 acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat
     8521 gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca
     8581 gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc
     8641 gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc
     8701 agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc
     8761 cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga
     8821 gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac
     8881 acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa
     8941 agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa
     9001 tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag
     9061 agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat
     9121 tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga
     9181 ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc
     9241 ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt
     9301 gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa
     9361 ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt
     9421 ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg
     9481 tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta
     9541 tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa
     9601 aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa
     9661 caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt
     9721 ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat
     9781 gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg
     9841 acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg
     9901 attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag
     9961 aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta
    10021 tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat ccaattgatt
    10081 ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg
    10141 tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac
    10201 agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt
    10261 tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag
    10321 atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc
    10381 gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa
    10441 cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt
    10501 gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc
    10561 ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc
    10621 cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga
    10681 caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc
    10741 gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt
    10801 gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac
    10861 ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta
    10921 ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat
    10981 ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt
    11041 aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg
    11101 tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt tttattgaaa
    11161 ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata tttttttatt
    11221 ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa
    11281 attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg
    11341 ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg atcaactgga
    11401 agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa
    11461 agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt tgcttcattt
    11521 gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta
    11581 aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata agcaactcgc
    11641 aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataaa
    11701 ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca
    11761 tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg
    11821 tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc
    11881 ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca
    11941 attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat
    12001 cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca
    12061 ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt
    12121 aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc
    12181 aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta
    12241 cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta
    12301 gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta
    12361 tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa
    12421 tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc
    12481 tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt
    12541 tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctcc
    12601 actccttaaa attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa
    12661 tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct
    12721 tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt
    12781 aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg caaaaatatg
    12841 tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa agtcattacc
    12901 gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt
    12961 gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga
    13021 acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt
    13081 gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt
    13141 cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct
    13201 aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca
    13261 gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa
    13321 gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga
    13381 gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctgcc
    13441 ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt
    13501 attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg
    13561 aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat
    13621 atttataaca aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg
    13681 catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc
    13741 tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta
    13801 cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc
    13861 tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg
    13921 atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt
    13981 attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg tctgaatatg
    14041 aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat
    14101 tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg
    14161 aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa
    14221 ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc
    14281 ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat
    14341 caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag
    14401 cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa
    14461 attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac gcaaactttt
    14521 tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt ttattcaatc
    14581 aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata accatcaggg
    14641 tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc agtaatctca
    14701 agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc tatagcgata
    14761 tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat
    14821 tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca
    14881 cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga
    14941 tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct tttcctttta
    15001 ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca tgagaaattt
    15061 ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat ggataaacca
    15121 atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc atatattgtc
    15181 atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt ttttcttcct
    15241 ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa
    15301 gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac
    15361 tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg
    15421 aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa
    15481 gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa
    15541 aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag
    15601 tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt
    15661 tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc
    15721 aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc
    15781 aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc
    15841 gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg
    15901 cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat
    15961 gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc
    16021 gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga
    16081 atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt
    16141 cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata
    16201 agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc
    16261 tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat
    16321 cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc
    16381 gatagcgact agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa
    16441 tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg
    16501 atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc
    16561 ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc
    16621 atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata
    16681 aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc cgaccttttc
    16741 tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga
    16801 tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga
    16861 ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc
    16921 taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct
    16981 ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat
    17041 accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata
    17101 atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat
    17161 cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc
    17221 ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg
    17281 tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac
    17341 tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc
    17401 ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc
    17461 atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc
    17521 tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata
    17581 aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt
    17641 gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca aatctaagat
    17701 tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg
    17761 cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt
    17821 ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg
    17881 tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt
    17941 tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag
    18001 gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc
    18061 gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac atcataacag
    18121 aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta
    18181 aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg
    18241 gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt
    18301 ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct gatacgatcc
    18361 ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt gtttggatcc
    18421 cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt
    18481 gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta
    18541 atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt
    18601 tcattcaacc ggctcctttg tataaattca tattttctac aatattatac tctaaaatca
    18661 aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa
    18721 agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca
    18781 aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga
    18841 tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta
    18901 taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga
    18961 tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa
    19021 agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac
    19081 gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc gaatcgatta
    19141 tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc
    19201 gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact
    19261 gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt
    19321 atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc
    19381 tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa ttcgtgattc
    19441 actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa
    19501 tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg
    19561 aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt
    19621 tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct
    19681 gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc
    19741 tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga
    19801 cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga
    19861 ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag
    19921 gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgatt tttgtcacac
    19981 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
    20041 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
    20101 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
    20161 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
    20221 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
    20281 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
    20341 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
    20401 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
    20461 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
    20521 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
    20581 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
    20641 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
    20701 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
    20761 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
    20821 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
    20881 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
    20941 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
    21001 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
    21061 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
    21121 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
    21181 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
    21241 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
    21301 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
    21361 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
    21421 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
    21481 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
    21541 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
    21601 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
    21661 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
    21721 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
    21781 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
    21841 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
    21901 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
    21961 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
    22021 cagacagctg aagcagggca cgagattggc aattacacat acgatcatca gaatctggcc
    22081 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
    22141 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
    22201 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
    22261 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
    22321 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
    22381 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
    22441 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
    22501 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
    22561 caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc
    22621 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
    22681 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
    22741 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
    22801 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
    22861 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
    22921 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
    22981 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
    23041 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
    23101 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
    23161 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
    23221 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
    23281 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
    23341 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
    23401 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
    23461 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
    23521 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa
    23581 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
    23641 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg
    23701 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
    23761 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
    23821 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag atttcggtgt
    23881 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
    23941 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
    24001 aaacaagccg ttgtcggacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
    24061 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
    24121 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc
    24181 tttttagttg aaaaacgaga gcgaaactgt tagacttaca gtcgtgaggt tgagatttcc
    24241 ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta
    24301 agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa
    24361 tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac
    24421 gaaatatcta ttggatgcag ccgattgggc gtcgaagttt atcggaaagt atggggatca
    24481 cagaacgagt cttacgaaca gaaacagacc ttttgaaaaa tctgaatttg atcgacacct
    24541 caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact
    24601 tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta
    24661 accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat
    24721 tcggcgaagc gctgagcgaa gccttagacc gactacttcc agaaggcgag aatatcatcg
    24781 ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata
    24841 agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac
    24901 aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgcgcacttt
    24961 atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc
    25021 aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat
    25081 tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa
    25141 atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg
    25201 tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg
    25261 cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa
    25321 cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa
    25381 ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt
    25441 aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc
    25501 gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac
    25561 gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta
    25621 aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa
    25681 ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa
    25741 aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt
    25801 ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca
    25861 ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt
    25921 ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta
    25981 gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt
    26041 cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcccgaatt gaacggtaaa
    26101 ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca
    26161 gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac
    26221 gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac
    26281 ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt
    26341 aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta
    26401 gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca
    26461 tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg
    26521 aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct
    26581 tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat
    26641 caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt
    26701 ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt
    26761 tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg
    26821 agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa
    26881 tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa
    26941 atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc
    27001 acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat
    27061 ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc
    27121 aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa
    27181 agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat
    27241 caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga
    27301 aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa
    27361 tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat
    27421 cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg
    27481 gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg
    27541 tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagccgc
    27601 agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag gtggcggcgc
    27661 aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata
    27721 aacgaccaag tttgctcaat tctgacgagt aatcgaacaa aaggtgaagg gagttttcat
    27781 gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta
    27841 tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag
    27901 taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat
    27961 tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact
    28021 gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc
    28081 taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag
    28141 acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat
    28201 gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac
    28261 aaattacagc tggtttagct gatctttcag ctgaacaggt gtcaaacatg gttattgctt
    28321 atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa
    28381 tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag
    28441 ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag
    28501 aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca ttcttgtcat
    28561 tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact
    28621 aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca
    28681 aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca
    28741 cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga
    28801 atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac
    28861 aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt
    28921 gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc
    28981 gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct
    29041 atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc
    29101 ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat
    29161 gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc
    29221 aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct
    29281 cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac
    29341 gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa
    29401 gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt
    29461 tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac
    29521 gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga cgaaaacgac
    29581 tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac
    29641 gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac
    29701 tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa
    29761 atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat
    29821 tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac tggttctcta
    29881 tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt
    29941 gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt
    30001 tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt
    30061 aatcgaactc tctacttgct gaaatatcag caggtagaga gtttttttgc aactagcttc
    30121 tgtatatgat gtttacaaag aatggtacaa gaaaagatca tccttgaaaa aggaaagcta
    30181 acaataacta gcatcatata agagctttta aggatagaac agtagaatag gaatctatgg
    30241 gcaagtcctc tcgtataaat aaaataggag ggaagtagca gcatattttt cataaagtgg
    30301 ctctctttct tcatttttac tttggttgct cctatttgaa aattcgagca cactcaaata
    30361 caataaaaag aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa atcgcgattt
    30421 tacaagagat tatccgaatt aaatcagtga atggaaacga aggtgaagta gcagcctatt
    30481 tgaataagct tttggcaaag catgacataa caggagagat tgtttcttac agagatggta
    30541 gagataattt gattgctcgt taccaaaagg gccaatcagg aaaagtgtta ggcctatctg
    30601 gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca ccctttgcag
    30661 cagaaatcca tgggaaccgt ttgtacggac gtggtgctac agatatgaag agtggcttgg
    30721 cagcaatggt gattgccatg atcgaactga aagagtcagg aaaaccattt aacggaacag
    30781 tcaaattatt agccactgtc ggagaagaag tgggtgaact aggtggggaa cagctcacga
    30841 aagcaggcta tgtagatgac ttagatgcct tgatcatcgg agagccaacc aattacagtt
    30901 tgatgtatac gcacatggga tcaatcaact atacagtcac ctcgcacgga aaagaagcgc
    30961 atagctctat gccagaccaa ggttataatg caatcaatca cctaaatgaa ttcattacga
    31021 aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga gaatcctgtc ctaggaaaaa
    31081 caatccataa tgttacattg atcagtggcg ggaatcaagt caacagcatc ccaagccatg
    31141 cgcagctcca agggaacatt cgatcgattc cagaataccc aaatgataaa atcattgcat
    31201 tgctgcaatc aatcgtcaat gaattgaatc aagagacaga ctatcatttg gaattgatga
    31261 tcgattacaa caaaatacct gtcaaagctg atccagattc tcctttgatt cattgcattc
    31321 agcaacaatt cagccagcca ttacctttag ttggagcggc agcaacaacg gatgcagccg
    31381 aatttacgaa agctaaccat tccttcgact ttgtcgtctt tgggcctggt gtcgttactt
    31441 tgcctcatca agtagatgag tatgttgaaa tcgataatta tttggatatg atcgaaaaat
    31501 atcaaggaat tattttatcc tacttagcgt aagatttttt taat
//
LOCUS       DBNIBO010000012        31434 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.012, whole genome shotgun sequence.
ACCESSION   DBNIBO010000012 DBNIBO010000000
VERSION     DBNIBO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.012"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(23..490)
                     /locus_tag="AC1Y77_000543"
     CDS             complement(23..490)
                     /locus_tag="AC1Y77_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIQ1574147.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(610..2148)
                     /locus_tag="AC1Y77_000544"
     CDS             complement(610..2148)
                     /locus_tag="AC1Y77_000544"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - obsolete D-gluconate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HIQ1574148.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2228..2914
                     /locus_tag="AC1Y77_000545"
     CDS             2228..2914
                     /locus_tag="AC1Y77_000545"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIQ1574149.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="AC1Y77_000546"
     CDS             complement(3115..3642)
                     /gene="msrA"
                     /locus_tag="AC1Y77_000546"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIQ1574150.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="AC1Y77_000547"
     CDS             complement(3726..4403)
                     /gene="pgmB"
                     /locus_tag="AC1Y77_000547"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIQ1574151.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4400..5530)
                     /locus_tag="AC1Y77_000548"
     CDS             complement(4400..5530)
                     /locus_tag="AC1Y77_000548"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIQ1574152.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5530..7713)
                     /locus_tag="AC1Y77_000549"
     CDS             complement(5530..7713)
                     /locus_tag="AC1Y77_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIQ1574153.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7710..8735)
                     /locus_tag="AC1Y77_000550"
     CDS             complement(7710..8735)
                     /locus_tag="AC1Y77_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1574154.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8780..9607)
                     /locus_tag="AC1Y77_000551"
     CDS             complement(8780..9607)
                     /locus_tag="AC1Y77_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIQ1574155.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9604..10671)
                     /locus_tag="AC1Y77_000552"
     CDS             complement(9604..10671)
                     /locus_tag="AC1Y77_000552"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIQ1574156.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10697..11533)
                     /locus_tag="AC1Y77_000553"
     CDS             complement(10697..11533)
                     /locus_tag="AC1Y77_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574157.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11530..12399)
                     /locus_tag="AC1Y77_000554"
     CDS             complement(11530..12399)
                     /locus_tag="AC1Y77_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574158.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12469..13752)
                     /locus_tag="AC1Y77_000555"
     CDS             complement(12469..13752)
                     /locus_tag="AC1Y77_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574159.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13766..15451)
                     /locus_tag="AC1Y77_000556"
     CDS             complement(13766..15451)
                     /locus_tag="AC1Y77_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIQ1574160.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15726..16751
                     /locus_tag="AC1Y77_000557"
     CDS             15726..16751
                     /locus_tag="AC1Y77_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574161.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="AC1Y77_000558"
     CDS             complement(17346..20999)
                     /gene="rpoC"
                     /locus_tag="AC1Y77_000558"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIQ1574162.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="AC1Y77_000559"
     CDS             complement(21078..24695)
                     /gene="rpoB"
                     /locus_tag="AC1Y77_000559"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIQ1574163.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25423..26391
                     /locus_tag="AC1Y77_000560"
     CDS             25423..26391
                     /locus_tag="AC1Y77_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574164.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26409..27314
                     /locus_tag="AC1Y77_000561"
     CDS             26409..27314
                     /locus_tag="AC1Y77_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574165.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27311..28117
                     /locus_tag="AC1Y77_000562"
     CDS             27311..28117
                     /locus_tag="AC1Y77_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574166.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28274..29242
                     /locus_tag="AC1Y77_000563"
     CDS             28274..29242
                     /locus_tag="AC1Y77_000563"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIQ1574167.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29559..30083)
                     /locus_tag="AC1Y77_000564"
     CDS             complement(29559..30083)
                     /locus_tag="AC1Y77_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIQ1574168.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30370..>31434
                     /gene="scm"
                     /locus_tag="AC1Y77_000565"
     CDS             30370..>31434
                     /gene="scm"
                     /locus_tag="AC1Y77_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HIQ1574169.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8822 a         6785 c         5922 g         9905 t
ORIGIN      
        1 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
       61 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
      121 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
      181 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
      241 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
      301 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
      361 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
      421 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
      481 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
      541 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
      601 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
      661 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
      721 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
      781 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
      841 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
      901 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
      961 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
     1021 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
     1081 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
     1141 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
     1201 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
     1261 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
     1321 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
     1381 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
     1441 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
     1501 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
     1561 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
     1621 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
     1681 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
     1741 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
     1801 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
     1861 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga
     1921 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
     1981 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
     2041 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
     2101 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
     2161 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
     2221 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
     2281 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
     2341 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
     2401 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
     2461 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
     2521 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
     2581 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
     2641 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
     2701 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
     2761 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
     2821 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
     2881 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
     2941 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
     3001 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
     3061 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
     3121 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
     3181 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
     3241 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
     3301 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
     3361 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
     3421 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
     3481 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
     3541 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
     3601 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
     3661 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
     3721 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
     3781 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
     3841 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
     3901 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
     3961 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
     4021 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
     4081 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
     4141 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
     4201 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
     4261 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
     4321 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
     4381 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
     4441 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
     4501 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
     4561 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
     4621 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
     4681 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
     4741 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
     4801 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
     4861 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
     4921 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
     4981 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
     5041 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
     5101 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
     5161 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
     5221 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
     5281 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
     5341 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
     5401 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
     5461 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
     5521 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
     5581 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
     5641 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
     5701 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
     5761 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
     5821 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
     5881 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
     5941 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
     6001 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
     6061 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
     6121 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
     6181 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
     6241 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
     6301 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
     6361 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
     6421 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
     6481 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
     6541 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
     6601 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
     6661 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
     6721 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
     6781 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
     6841 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
     6901 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
     6961 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
     7021 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
     7081 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
     7141 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
     7201 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
     7261 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
     7321 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
     7381 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
     7441 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
     7501 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
     7561 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
     7621 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
     7681 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
     7741 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
     7801 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
     7861 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
     7921 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
     7981 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
     8041 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
     8101 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
     8161 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
     8221 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
     8281 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
     8341 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
     8401 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
     8461 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
     8521 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
     8581 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
     8641 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
     8701 acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct ttgcttattt
     8761 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
     8821 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
     8881 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
     8941 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
     9001 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
     9061 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
     9121 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
     9181 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
     9241 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
     9301 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
     9361 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
     9421 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
     9481 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
     9541 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
     9601 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
     9661 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
     9721 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
     9781 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
     9841 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
     9901 ggcaacataa gaaatgatcc cgccatagcc gatccctatt aaagaagctt gtaatgcggc
     9961 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    10021 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt gtttttctgc
    10081 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    10141 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    10201 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    10261 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    10321 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    10381 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    10441 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    10501 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    10561 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    10621 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    10681 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    10741 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    10801 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    10861 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    10921 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    10981 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    11041 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    11101 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    11161 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    11221 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    11281 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    11341 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    11401 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    11461 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    11521 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    11581 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    11641 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    11701 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    11761 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    11821 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    11881 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    11941 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    12001 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    12061 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    12121 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    12181 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    12241 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    12301 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    12361 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    12421 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    12481 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    12541 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    12601 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    12661 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    12721 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    12781 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    12841 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    12901 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    12961 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    13021 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    13081 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    13141 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    13201 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    13261 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    13321 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    13381 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    13441 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    13501 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    13561 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    13621 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    13681 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    13741 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    13801 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    13861 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    13921 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    13981 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    14041 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    14101 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    14161 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    14221 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    14281 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    14341 ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    14401 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    14461 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    14521 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    14581 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    14641 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    14701 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    14761 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    14821 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    14881 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    14941 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    15001 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    15061 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    15121 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    15181 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    15241 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    15301 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    15361 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    15421 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    15481 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    15541 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    15601 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    15661 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    15721 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    15781 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    15841 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    15901 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    15961 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    16021 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    16081 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    16141 aattggatca atataatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    16201 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    16261 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    16321 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    16381 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    16441 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    16501 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    16561 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    16621 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    16681 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    16741 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    16801 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    16861 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    16921 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    16981 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    17041 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    17101 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    17161 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    17221 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    17281 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    17341 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    17401 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    17461 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    17521 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    17581 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    17641 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    17701 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    17761 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    17821 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    17881 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    17941 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    18001 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    18061 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    18121 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    18181 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    18241 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    18301 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    18361 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    18421 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    18481 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    18541 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    18601 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    18661 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    18721 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    18781 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    18841 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    18901 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    18961 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    19021 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    19081 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    19141 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    19201 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    19261 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    19321 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    19381 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    19441 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    19501 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    19561 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    19621 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    19681 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    19741 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    19801 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    19861 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    19921 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    19981 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    20041 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    20101 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    20161 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    20221 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    20281 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    20341 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    20401 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    20461 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    20521 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    20581 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    20641 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    20701 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    20761 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    20821 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc acgttcaggt
    20881 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    20941 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    21001 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    21061 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    21121 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    21181 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    21241 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    21301 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    21361 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    21421 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    21481 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    21541 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    21601 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    21661 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    21721 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    21781 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    21841 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    21901 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    21961 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    22021 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    22081 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    22141 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    22201 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    22261 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    22321 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    22381 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    22441 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    22501 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    22561 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    22621 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    22681 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    22741 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    22801 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    22861 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    22921 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    22981 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    23041 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    23101 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    23161 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    23221 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    23281 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    23341 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    23401 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    23461 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    23521 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    23581 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    23641 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    23701 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    23761 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    23821 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    23881 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    23941 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    24001 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    24061 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    24121 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    24181 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    24241 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    24301 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    24361 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    24421 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    24481 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    24541 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    24601 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    24661 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    24721 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    24781 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    24841 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    24901 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    24961 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    25021 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    25081 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    25141 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    25201 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    25261 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    25321 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    25381 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    25441 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    25501 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    25561 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    25621 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    25681 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    25741 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    25801 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    25861 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    25921 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    25981 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    26041 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    26101 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    26161 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    26221 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    26281 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    26341 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    26401 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    26461 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    26521 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    26581 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    26641 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    26701 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    26761 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    26821 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    26881 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    26941 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    27001 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    27061 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    27121 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    27181 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    27241 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    27301 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    27361 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    27421 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    27481 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    27541 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    27601 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    27661 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    27721 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    27781 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    27841 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    27901 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    27961 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    28021 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    28081 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    28141 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    28201 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    28261 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    28321 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    28381 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    28441 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    28501 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    28561 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    28621 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    28681 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    28741 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    28801 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    28861 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    28921 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    28981 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    29041 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    29101 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    29161 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    29221 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    29281 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    29341 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    29401 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    29461 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    29521 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    29581 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    29641 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    29701 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    29761 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    29821 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    29881 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    29941 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    30001 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    30061 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    30121 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    30181 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    30241 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    30301 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    30361 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    30421 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    30481 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    30541 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    30601 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    30661 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    30721 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    30781 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    30841 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    30901 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    30961 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    31021 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    31081 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    31141 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    31201 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    31261 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    31321 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    31381 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DBNIBO010000013        30097 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.013, whole genome shotgun sequence.
ACCESSION   DBNIBO010000013 DBNIBO010000000
VERSION     DBNIBO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.013"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            227..715
                     /gene="purE"
                     /locus_tag="AC1Y77_000566"
     CDS             227..715
                     /gene="purE"
                     /locus_tag="AC1Y77_000566"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIQ1574170.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            708..1832
                     /gene="purK"
                     /locus_tag="AC1Y77_000567"
     CDS             708..1832
                     /gene="purK"
                     /locus_tag="AC1Y77_000567"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIQ1574171.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1845..3140
                     /gene="purB"
                     /locus_tag="AC1Y77_000568"
     CDS             1845..3140
                     /gene="purB"
                     /locus_tag="AC1Y77_000568"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIQ1574172.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3320..3697)
                     /locus_tag="AC1Y77_000569"
     CDS             complement(3320..3697)
                     /locus_tag="AC1Y77_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574173.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3953..4681
                     /gene="purC"
                     /locus_tag="AC1Y77_000570"
     CDS             3953..4681
                     /gene="purC"
                     /locus_tag="AC1Y77_000570"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIQ1574174.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4681..4935
                     /gene="purS"
                     /locus_tag="AC1Y77_000571"
     CDS             4681..4935
                     /gene="purS"
                     /locus_tag="AC1Y77_000571"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIQ1574175.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4937..5608
                     /gene="purQ"
                     /locus_tag="AC1Y77_000572"
     CDS             4937..5608
                     /gene="purQ"
                     /locus_tag="AC1Y77_000572"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIQ1574176.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNSKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5609..7831
                     /gene="purL"
                     /locus_tag="AC1Y77_000573"
     CDS             5609..7831
                     /gene="purL"
                     /locus_tag="AC1Y77_000573"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIQ1574177.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIAEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7816..9255
                     /gene="purF"
                     /locus_tag="AC1Y77_000574"
     CDS             7816..9255
                     /gene="purF"
                     /locus_tag="AC1Y77_000574"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIQ1574178.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLMGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKNTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLKKDGPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9278..10330
                     /gene="purM"
                     /locus_tag="AC1Y77_000575"
     CDS             9278..10330
                     /gene="purM"
                     /locus_tag="AC1Y77_000575"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIQ1574179.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPIRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            10327..10905
                     /gene="purN"
                     /locus_tag="AC1Y77_000576"
     CDS             10327..10905
                     /gene="purN"
                     /locus_tag="AC1Y77_000576"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIQ1574180.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKELEASIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLENYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEGKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10902..12437
                     /gene="purH"
                     /locus_tag="AC1Y77_000577"
     CDS             10902..12437
                     /gene="purH"
                     /locus_tag="AC1Y77_000577"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIQ1574181.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDQAGIN
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQKREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPNPAEAKALAFAWKTVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12453..13727
                     /gene="purD"
                     /locus_tag="AC1Y77_000578"
     CDS             12453..13727
                     /gene="purD"
                     /locus_tag="AC1Y77_000578"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIQ1574182.1"
                     /translation="MNVLVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGNSSKKIVVEEFLAGEEFSLLAFVRDTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQIPKKMIEEAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRAVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFPEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQE
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13849..14763
                     /locus_tag="AC1Y77_000579"
     CDS             13849..14763
                     /locus_tag="AC1Y77_000579"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIQ1574183.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14893..16461)
                     /locus_tag="AC1Y77_000580"
     CDS             complement(14893..16461)
                     /locus_tag="AC1Y77_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1574184.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSNELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLLVLTKLLQHICLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNNQ"
     gene            complement(16454..17905)
                     /locus_tag="AC1Y77_000581"
     CDS             complement(16454..17905)
                     /locus_tag="AC1Y77_000581"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1574185.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPFEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17919..18935)
                     /locus_tag="AC1Y77_000582"
     CDS             complement(17919..18935)
                     /locus_tag="AC1Y77_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574186.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECIPDVGWYIANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19155..20243
                     /gene="chvE"
                     /locus_tag="AC1Y77_000583"
     CDS             19155..20243
                     /gene="chvE"
                     /locus_tag="AC1Y77_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HIQ1574187.1"
                     /translation="MKNKFRKIVGSIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20297..21823
                     /gene="mmsA"
                     /locus_tag="AC1Y77_000584"
     CDS             20297..21823
                     /gene="mmsA"
                     /locus_tag="AC1Y77_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIQ1574188.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21825..23030
                     /gene="mmsB"
                     /locus_tag="AC1Y77_000585"
     CDS             21825..23030
                     /gene="mmsB"
                     /locus_tag="AC1Y77_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HIQ1574189.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPAAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23177..24604
                     /locus_tag="AC1Y77_000586"
     CDS             23177..24604
                     /locus_tag="AC1Y77_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HIQ1574190.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            24840..25817
                     /gene="guaC"
                     /locus_tag="AC1Y77_000587"
     CDS             24840..25817
                     /gene="guaC"
                     /locus_tag="AC1Y77_000587"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIQ1574191.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26079..27503
                     /locus_tag="AC1Y77_000588"
     CDS             26079..27503
                     /locus_tag="AC1Y77_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIQ1574192.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQMD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISVWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDETQIPLFYSKMKILEKWLTE"
     gene            complement(27545..27802)
                     /locus_tag="AC1Y77_000589"
     CDS             complement(27545..27802)
                     /locus_tag="AC1Y77_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574193.1"
                     /translation="MPIRILTALVNIITIRKAFYISSAFFLFSNQTYNHLFLFRNVGV
                     IQFCCKYLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            27964..29073
                     /gene="pdhA"
                     /locus_tag="AC1Y77_000590"
     CDS             27964..29073
                     /gene="pdhA"
                     /locus_tag="AC1Y77_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIQ1574194.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29076..30053
                     /locus_tag="AC1Y77_000591"
     CDS             29076..30053
                     /locus_tag="AC1Y77_000591"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIQ1574195.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9743 a         5146 c         6733 g         8475 t
ORIGIN      
        1 agcttctcat gtttgttgaa tattgttcct tgtgaccgag gagttggcag gagaacaccg
       61 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt tatctttaat
      121 aaacacgaac aataaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
      181 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
      241 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
      301 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
      361 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
      421 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
      481 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
      541 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
      601 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
      661 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
      721 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
      781 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
      841 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
      901 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt gtagaggcgt
      961 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
     1021 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
     1081 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
     1141 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
     1201 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
     1261 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
     1321 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
     1381 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
     1441 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
     1501 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
     1561 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
     1621 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
     1681 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
     1741 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
     1801 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
     1861 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
     1921 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
     1981 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
     2041 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
     2101 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
     2161 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
     2221 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
     2281 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
     2341 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggga
     2401 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
     2461 cacaagaaat ctctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
     2521 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
     2581 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
     2641 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
     2701 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
     2761 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
     2821 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
     2881 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
     2941 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
     3001 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
     3061 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
     3121 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
     3181 aaaataacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
     3241 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
     3301 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
     3361 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
     3421 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
     3481 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
     3541 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
     3601 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
     3661 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
     3721 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
     3781 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgtctta
     3841 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaacac
     3901 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
     3961 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
     4021 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
     4081 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
     4141 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
     4201 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
     4261 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
     4321 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
     4381 aaaaccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
     4441 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
     4501 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
     4561 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
     4621 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
     4681 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
     4741 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
     4801 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
     4861 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
     4921 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
     4981 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
     5041 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
     5101 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
     5161 tgctgaagaa gggaaaccgg tattcggtac atgcaatgga ttccagatat tgacagaagc
     5221 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
     5281 accattgaaa gtcaactcca agacggcctt tacttcagag tatgaagccg acgaaataat
     5341 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
     5401 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
     5461 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
     5521 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
     5581 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
     5641 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
     5701 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tctctgttat
     5761 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
     5821 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
     5881 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagctgtgg agccttatga
     5941 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
     6001 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
     6061 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
     6121 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
     6181 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
     6241 gatcatgtat gtaggcgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
     6301 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
     6361 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
     6421 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
     6481 aaaagctggt tcaggactta tattgacatt agatgaagtc ccacaaagag aaactggaat
     6541 gacgccttat gaaatgatgc tatcggaatc acaagaacgg atgttgattt gtgtcaaatc
     6601 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
     6661 cgggaaagta acagatgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
     6721 tctgccagtc gatgcattag ctgaagatgc accagtctat cataaagaaa tgaaagaacc
     6781 agctcgaatc gcagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
     6841 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcgaaacgtt caatctatga
     6901 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg acgctgctgt
     6961 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
     7021 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
     7081 tattgctagc ggtggcaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
     7141 taaaccagaa gttttttggg aattatcgac ttcagctgat ggaattgctg ctgcgtgtga
     7201 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
     7261 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
     7321 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
     7381 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
     7441 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ctatccaaaa
     7501 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
     7561 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
     7621 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
     7681 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
     7741 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
     7801 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
     7861 ggcgtttggg gtcatccgga tgccgctagt gtggcatact tcgggctcca tagtcttcag
     7921 catcgaggac aagaaggtgc gggcatcgtg tccaatgatc gaggaaagct tatgggtcat
     7981 cgagatctgg ggctgctttc tgaggtattc aaagatcaaa gaaaactgca gcagttaaca
     8041 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
     8101 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taacggcaat
     8161 ttgatcaatg ctcgttcact tagaaaagag ttagaagaaa atggagcgat cttccattct
     8221 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
     8281 aataaattga aaagtgcttt aaatgaagtc aaaggtggct ttgcctattt attaatgaca
     8341 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aattgggcaa
     8401 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tatcggggca
     8461 cgttttataa gagatgtcca gcctggagag atagtgatca tcaatgacga aggaatccag
     8521 attgattgct ttacgaagaa tacgcagctt tccatttgtt cgatggaata tatctatttt
     8581 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
     8641 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgtcggcgt acctaattct
     8701 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
     8761 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
     8821 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
     8881 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
     8941 gcaggagcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
     9001 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
     9061 atcaaagaat gtatcggtgc agattcttta ggatttttaa gtgaagaggg tctgatcgaa
     9121 tcgattggac tgaaaaagga tggcccgtat tcaggattat gtatggctta tttcaacgga
     9181 gattatccaa cgccgttata tgactatgaa gaaaagtatc aggaatcgct aaaagaaaaa
     9241 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
     9301 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
     9361 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
     9421 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggtgtggg
     9481 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
     9541 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
     9601 cttagcaacg gggaaaaatg atccgatacg tttggaaaat gtagtaaaag gtgtagcaga
     9661 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
     9721 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
     9781 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
     9841 gatccattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
     9901 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
     9961 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    10021 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    10081 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    10141 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gcatggtcct
    10201 ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    10261 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    10321 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    10381 gattatctat cgaaaaaaga attggaagct tcgattgact ggttgttttg tgatcagcct
    10441 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    10501 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    10561 aagatcgatt tgattgtatt agctggatac atgcgaatca ttggaccagt attgctggaa
    10621 aattatgata aaagaatcat caatatccat ccatctcttt tacctgcatt ccctggtttg
    10681 catggcattc gtgatgcatt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    10741 atcgaccaag gtgtagatac cggaccaatc atccggcagg aaaaagtgag aattgaacag
    10801 gaggacacgt tcgattcttt agaaggaaaa atccatagag tagagcatcg aatctatcca
    10861 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    10921 gtgtatcaga taaaactggg attgtagcat ttgcccaagg tttgattgaa caaggagtag
    10981 aaatcatttc aacaggagga acaaagcatg cgttggatca agctgggatc aatacgcttt
    11041 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    11101 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    11161 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    11221 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    11281 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    11341 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    11401 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    11461 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    11521 gaaaacaaac gcttcgttac ggtgaaaaca gccatcaagc cgctgccttt tatcagtccg
    11581 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    11641 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    11701 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    11761 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    11821 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    11881 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    11941 gattactgac agtagacttt gcgcaggaag aaaatcaaaa gcgagaagaa acggtctcag
    12001 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    12061 aagtcgtgac aaaacgtaag ccgaatccag cagaagcaaa agcattagcg tttgcttgga
    12121 aaactgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    12181 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    12241 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    12301 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    12361 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    12421 ggcattttag acattagaga ggagtcggat aaatgaacgt tttggtaatc ggtgcaggtg
    12481 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    12541 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    12601 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    12661 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    12721 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    12781 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    12841 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcagat ggcttagcag
    12901 ctggaaaagg tgtagtcgtg gctacaacag tgaaggaagc gctggatgcg gcagaacaaa
    12961 tgttgaacca gcatcgattt gggaacagca gcaaaaaaat cgtcgtagaa gagtttttgg
    13021 caggagaaga attctccctt ttggcgtttg tccgtgatac caaggtgtat ccgatggtca
    13081 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    13141 gtgcttattc accagtaccg caaattccaa aaaagatgat tgaagaagct gtggaagaag
    13201 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    13261 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    13321 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    13381 atgacttatt agagaatcgt gcagtagagt taaagtggca gcaagaagga tacaatgtag
    13441 gcgttgtggt ggcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    13501 attttccgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    13561 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    13621 cacaggaaaa aatctacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    13681 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    13741 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    13801 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    13861 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaat
    13921 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    13981 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    14041 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    14101 gtgatgtttg atttttctaa aaaacatatc attgaatctg tggaaaaaag tttgacccgt
    14161 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    14221 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    14281 gtcagcaatc aaaaaccaat gcagatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    14341 gtagctaatc agctccaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    14401 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    14461 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    14521 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    14581 tatcaaacga caccaactgg cttggcttct gcttggattt tgcgccatcc agccaatatg
    14641 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    14701 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    14761 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    14821 gctgttttcg gcttcaagat attctgtctt ttttaattgc aaatggaaag agtatcattg
    14881 tacggatttc gtttattggt tatttttgtt tttaaggaaa agttgtgtcc gatattcttt
    14941 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    15001 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    15061 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    15121 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    15181 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    15241 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    15301 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    15361 catttcttgc gggattttct tttctttcag gcagatatgc tggagtagct tagtcaagac
    15421 aagtaaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttctat
    15481 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    15541 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    15601 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    15661 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    15721 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    15781 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    15841 attctcccta aataaaaact ttaaataatc actgagatga gcgtaatctt caaagtctgt
    15901 actaacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    15961 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    16021 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    16081 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgttac ttttaatcgg
    16141 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    16201 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    16261 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    16321 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    16381 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    16441 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    16501 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    16561 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    16621 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggattc
    16681 caatcccgtt gtctatgact ttgaattgga tcatctcatc gattttgcat gcggtaatac
    16741 gaataaatcc tttgtgacgg acgtacttcg ttccatggta cacagcattt tcaatcaatg
    16801 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    16861 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    16921 tttctttttc aaaaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    16981 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    17041 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    17101 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    17161 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    17221 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    17281 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    17341 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    17401 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    17461 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    17521 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    17581 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    17641 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    17701 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    17761 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    17821 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    17881 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    17941 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    18001 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctatata ccagccgaca
    18061 tcaggtatac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    18121 tcttgtccat caatcgttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    18181 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    18241 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    18301 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    18361 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    18421 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    18481 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    18541 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    18601 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    18661 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    18721 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    18781 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    18841 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    18901 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    18961 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    19021 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    19081 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    19141 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggagtatc gctattttga
    19201 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    19261 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    19321 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    19381 tagaaaatca agtagcacaa atcgaaaata tgattacaaa aggggtagat acacttgtga
    19441 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    19501 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    19561 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    19621 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    19681 atgcactaat taattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    19741 agttggttgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    19801 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    19861 tggatgctgt tttgtcgcca tatgatccaa taagtttagg gatcatttcg tcattaaaag
    19921 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    19981 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    20041 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggtcc
    20101 cggtcaacga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    20161 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    20221 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    20281 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    20341 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    20401 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    20461 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    20521 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    20581 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    20641 tgattgggaa ctgacagaaa agaaaacgta tgaactgctg aataaagtcg gtttgtctat
    20701 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaattag ttgaaatagc
    20761 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    20821 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    20881 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    20941 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    21001 tattcgcggg atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    21061 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcatgagcg
    21121 aatcataaat gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    21181 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    21241 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    21301 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    21361 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    21421 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    21481 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc agcaaaaagt
    21541 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    21601 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    21661 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    21721 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    21781 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    21841 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    21901 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    21961 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    22021 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    22081 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    22141 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    22201 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    22261 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    22321 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    22381 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    22441 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccagcagct ttcttggtaa
    22501 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    22561 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    22621 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    22681 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    22741 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    22801 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    22861 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    22921 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    22981 ttttagccgt tgtgttagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    23041 gttattcaga aaaaagactg tgacaagaca tttgtcacag tcttttcaga ctgtagacaa
    23101 actcctaaat tgggagtgtt gtttgcagtt tttttgtttt atactcattg tataaactat
    23161 tctgggagtg attctaatga tgagaaaaca atcgattgaa ggtagaaatc aatttgctat
    23221 gttaacaatt gatgatcttg tacctaaaga tcacttagtt cgaaagattg atgctgcgat
    23281 tcagtttgat ttcatttatc ccattgtaga atctacgtat tcgacccatg gtcgtcccag
    23341 tattgatcca gtggtcttaa ttaaattagt atttatccaa tatttatttg gtatccgttc
    23401 gatgagacag actattaaag acgttgaaac aaatgttgct tatcgttggt ttttaggtta
    23461 ttcattcgag gatcctatcc cgcatttctc tacttttggt aaaaactatg ttcgaagatt
    23521 cagagaaaca actttatttg aagacatttt ctctcatatt ctagaacaag ctgtaaaagc
    23581 tggcttcgtt acggaagaca acctttatat tgattctact catattaagg caaatgccaa
    23641 taaacataag ttcacaaaag aaatgaccta cggtcaagct aaagcttatc aagatgaatt
    23701 agaagatgaa ataaataaag aacgtattgc tgtaggaaaa cgtcctttta catgggacac
    23761 agaaagtgag ttgagtcttc aaaaaataag tcattcagat ccagaaagtg gttactatgt
    23821 taaaggtgaa cgagaaaaac aatttgctta ttccgcgcat acttcctgtg aagataatgg
    23881 ttttatctta tctacattaa tcacaccagg aaatgtacat gacagccaag tcgcaatcga
    23941 tctggtcaaa cagtcgaaaa gagcttttcc aaatataaag cgtgttgtcg ctgatgcagg
    24001 atataagacg cctaaatttg ttcattttct gtcacacttg aaactctacc cgattcttcc
    24061 atatactgta cctcatggag taaaaggaat gtttcgtaaa aaagactttg tttatgatgc
    24121 ctattttgac tgctatttat gtcccaataa tcaaccgttg ttattttcaa ctatcactcg
    24181 tgatggttat cgaacctata aatcaaatcc aaaattctgt aggagttgtc cattacttaa
    24241 aaactgtact ttgagtacaa aacatcaaaa aatagtaact cgacatgttt gggcggatct
    24301 tatggatgaa cttgaacacc aacgccatac ggcgttgaat agagaaattt ataaaaagag
    24361 aaaacaaacc attgaacgaa tatttgcaga tgcaaaagaa aagcatggta tgcgttggac
    24421 gaaatatcga ggtctcgaaa aagtcgccac gcacacgatg cttacgtttg ctgccatgaa
    24481 tttaaaaaaa ctagccacat ggctatggaa agcaaaaaag cctatggctt tttatttaaa
    24541 atttctacaa agactaacaa aaaggagttg gcaattaagc cactcctttt tgtctacagt
    24601 ctgaaaagac tgtgacaaga catttgtcac agtctttttt tttgctaaaa gcgaacattc
    24661 attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct
    24721 ctttttgctc ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc
    24781 cttgattaaa ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa
    24841 tgaaagtatt tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc
    24901 gttctgaatg tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc
    24961 ctgccaacat gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt
    25021 atttctatat catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga
    25081 aaaagcgtgg attgattacc tcaatcagtg ttggcgtcaa gaaagaggaa tattccttta
    25141 tagagaagtt ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcacatg
    25201 gacatgctaa ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt
    25261 ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg
    25321 cagatgcaac aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag
    25381 gctttgggac tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca
    25441 agccgatcat cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt
    25501 ttggtgcgac aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg
    25561 aaacaaaagt cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc
    25621 aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg gatccagcat aaaggatctt
    25681 tgaaagatac actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag
    25741 gaagagatct ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta
    25801 atggagacac aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc
    25861 attaagtaat aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggact
    25921 tattcgcaga tacaagcttt tgaacatcat tattcagaat aagggactgt gataagactt
    25981 ttgtcacagt cccttatgtt ttgcaaaaaa agtttttctt ttttctcgta aattcggtaa
    26041 aatcaaaaga aaaaatagac aatagaaaag agaagcttat gtgggaaaaa gtagagaaaa
    26101 aacaaggacc gatttatcgt caaatcatgg aacagattat gagagaaatc gaaaacggaa
    26161 ggatcaatcc tggaagtcaa cttgaatcag aacgaaaact agggcaactt tttggtgtga
    26221 atcgttccac cgttgtccat gcattggaag agcttaggga gcttggggta ctaacgagca
    26281 aacgtggcag cggatggttt gttaatcaga ctgaatgggg gaaattcgct gttccaagaa
    26341 tcgattggcg gcaaatgatc tctcctcgtt atgagcagat ggactggtat gagcaaaaaa
    26401 taagagaagc aaagcagatc caaaaaacat cgtttcttga tttatacgct agcgagatgc
    26461 cagatgagct tttgccgaat tttgagtttc cttcttattc tcttgaagaa atcttagcgg
    26521 aagaaaaaga aatggatctt ttaggctata ctccgttacg taacaaaatc aaacggtatt
    26581 tggaaagtaa atttgaattg gcattttccc ccaaccagct tcttgtgact ggtggcggtc
    26641 agcaagcaat ctttttgatt ttacaaacga ttctttcagc tggagagggg atagctgtag
    26701 agtcaccttc ttttttttat cgattacctc ttttcaaagc ttcaggaacg cgtttatttg
    26761 ggataccgat ggatgaagaa gggatcgact tagaaaaact gaaagaatcg atccataaaa
    26821 ataaattgaa agcagtgctt atcaacccga attttcaaaa ccctacagga aaagtgatga
    26881 gtcaaaaacg acgagaaaag ctagtagaat tgtgtcgaca gtatcggctt ccaattattg
    26941 aagacgatgt atttacggat ctttcatttc taaatcaaga agaagtcctt cctatacgaa
    27001 cgattgatcc ggataatgtg ttatacgtcg gttcgttatc tagggtttta ggaaaaacaa
    27061 cgaaaatcgg ttgggtgata ggacctgaga cttttatctc gcaactagct caagcgcaag
    27121 aaatgatgga gttttcgatg aatatcctta cacaaatcac agtggctaat gtatttaatg
    27181 aagcaattga tttgaaaatg caagatgtaa gaaaaaagtt gaaagaaaaa agccgactga
    27241 taagcgtttg gggagattca caatcgtttt tcaagcttca ttcaatttta ggtggttact
    27301 atgcctggat tacttgggat gggaaaaaac tagaccccga actagcagaa aaagtagtag
    27361 atatgggtct tggaattgca ccaagtctac tttttgggca agatataaat ggaatcagaa
    27421 tcaattacag taggctggat gaaacccaaa taccgttgtt ttattcaaag atgaaaattc
    27481 tagaaaagtg gttaacagaa taatttaaca aagtaaatag attaaagaat ataacagctt
    27541 ggatttaaat ttcaagctgt ttcttttttt tcggaaaaag aatacgatat aaagaaacta
    27601 agaaaaaacc attatttgta cgtccgttca cgatgtgtaa ataaaataaa tatttgcaac
    27661 aaaactgtat aacaccaacg tttctaaata gaaacagatg gttgtaagtt tgattagaaa
    27721 atagaaaaaa tgcagatgaa atataaaacg ctttccttat tgtgattata ttcactaatg
    27781 ctgttaatat gcggattggc ataatttcct ttcatcgtga aaatttgtgc ttttagcttg
    27841 tatttctcat aaatccatga taatttatta atgaagacgg ttaaatagtg atacagtttt
    27901 attcactgtt tcaatcgcag aatgaagaga ttttcttata gatgagagag gaatgtgtga
    27961 caaatggcaa acaaaaaagc accgatcgac ttccaagcac tgctagatga agtcaatgca
    28021 gatttcccag tatatcaagc acttgatcaa gatggaaacg tagttaacaa agatttagta
    28081 cctgatttat ccgatgatga actcgttgaa ttaatgactc atatggtatg gtctcgtgta
    28141 ttagaccaac gttcaacagc gctgaaccgc caaggacgcc taggcttttt tgcaccaact
    28201 gctggacaag aggcaagtca gctggctagt gcttacgcat ttgataaaga agatgttctt
    28261 cttccaggtt atcgggatgt acctcaacta gtaaaacatg gtttaccttt atctcaagct
    28321 tttttatggt ctagaggaca tgctgcaggt aattcttatc cggaagaatt gaaagctcta
    28381 ccaccacaaa tcatcatcgg tgcacaatat gtacaagcag ccggtgttgc attaggtttg
    28441 aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa
    28501 ggtgattttt acgaagcaat caatttcgct ggcgcttacc atgcaaatgc cgtattttat
    28561 atccaaaaca acgggtatgc gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca
    28621 cttgcacaaa aagctgtcgc agctgggatt cctggtattc aagtcgatgg aatggatccg
    28681 cttgcagtat atactgtatc taaaatggca cgggaatggg caatcagtgg aaatggacca
    28741 gtcctaatcg aaacactgac ttatcgttat ggaccacata cactatctgg tgacgatcca
    28801 acccgctacc gtacaaaaga cacagatgac gattggcaga aaaaagatcc attgatccgc
    28861 ttccgtaaat atttgacgga aaaaggactt tggtctgaag aaaaagaaga acaagtcatc
    28921 gaagcaacta aagaagaaat caaagcagct attgctgaag ccgacaaagt accaaaacaa
    28981 aaggtttcag atttcttgaa aaacatgttc gaagtatctc cacaatcaat caaagaacaa
    29041 attgcaattt atgaagcgaa ggagtcgaaa taatcatggc acaaaaaaca atgatccaag
    29101 caatcacaga tgccttagca cttgaactag aaaatgacga gaatgtcgtt gtttttggtg
    29161 aagatgttgg gaaaaacgga ggagttttcc gggcaactga aggattgcag gaaaagttcg
    29221 gagaagaccg agtattcgat actcctttag ctgaatctgg aatcgcagga ttgagtttcg
    29281 gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca attctttggt tttattttcg
    29341 aagcaatgga cgaagtagta gctcaaatgg ctcgtacaag atatcgaatg agcggcacta
    29401 gaaatctacc catcacaatt cgttcgccat ttggtggcgg tgttcataca ccagaattgc
    29461 attcggataa tttagaaggt ttgatcgctc agtctcctgg tattcgagta gtgattcctt
    29521 ccaaccctta tgatgcaaaa ggactattga tttcttctat tagaagtaac gatccggttg
    29581 ttttcctaga acacatgaaa ctatatcgct cattccgaga agaagtgccg gatgaagctt
    29641 acgaagttcc tttggataaa gcagcagtta ctcgtgaggg aacagatgta tctattatca
    29701 catacggagc aatggtgcgt gaagcaatca aagcagcaga caatcttgca aaagaaaata
    29761 tatctgttga aatcattgat cttcgtacag ttgcgccatt agatgtagaa acgatcattc
    29821 aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga agcacaacga caagcgggcg
    29881 ttgccgcgca agtagtttct gagatctcag aacgcgctat cctttcatta gaagccccaa
    29941 tcggacgagt ttctgcacct gatacagtct tcccatttgg acaagcagaa aatgtctggt
    30001 tgccaaatgc aaaagatatc gaagacaaag taaaagaaat cgcggaattt taagaaaaga
    30061 agggacgaaa caaagtcatg gcttatcaat ttaaatt
//
LOCUS       DBNIBO010000014        29420 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.014, whole genome shotgun sequence.
ACCESSION   DBNIBO010000014 DBNIBO010000000
VERSION     DBNIBO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.014"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            5..553
                     /gene="hslV"
                     /locus_tag="AC1Y77_000592"
     CDS             5..553
                     /gene="hslV"
                     /locus_tag="AC1Y77_000592"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HIQ1574196.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            567..1967
                     /gene="hslU"
                     /locus_tag="AC1Y77_000593"
     CDS             567..1967
                     /gene="hslU"
                     /locus_tag="AC1Y77_000593"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIQ1574197.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1985..2776
                     /gene="codY"
                     /locus_tag="AC1Y77_000594"
     CDS             1985..2776
                     /gene="codY"
                     /locus_tag="AC1Y77_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIQ1574198.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2858..3733
                     /locus_tag="AC1Y77_000595"
     CDS             2858..3733
                     /locus_tag="AC1Y77_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIQ1574199.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="AC1Y77_000596"
     CDS             complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="AC1Y77_000596"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIQ1574200.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4630..5064
                     /locus_tag="AC1Y77_000597"
     CDS             4630..5064
                     /locus_tag="AC1Y77_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIQ1574201.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5230..7275
                     /gene="parE"
                     /locus_tag="AC1Y77_000598"
     CDS             5230..7275
                     /gene="parE"
                     /locus_tag="AC1Y77_000598"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIQ1574202.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSVKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7287..9737
                     /gene="parC"
                     /locus_tag="AC1Y77_000599"
     CDS             7287..9737
                     /gene="parC"
                     /locus_tag="AC1Y77_000599"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIQ1574203.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10014..12245
                     /gene="pflB"
                     /locus_tag="AC1Y77_000600"
     CDS             10014..12245
                     /gene="pflB"
                     /locus_tag="AC1Y77_000600"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIQ1574204.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12489..13250
                     /gene="pflA"
                     /locus_tag="AC1Y77_000601"
     CDS             12489..13250
                     /gene="pflA"
                     /locus_tag="AC1Y77_000601"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIQ1574205.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13335..14261
                     /locus_tag="AC1Y77_000602"
     CDS             13335..14261
                     /locus_tag="AC1Y77_000602"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HIQ1574206.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14443..15336
                     /locus_tag="AC1Y77_000603"
     CDS             14443..15336
                     /locus_tag="AC1Y77_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HIQ1574207.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15470..15595
                     /locus_tag="AC1Y77_000604"
     CDS             15470..15595
                     /locus_tag="AC1Y77_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HIQ1574208.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15729..16361)
                     /locus_tag="AC1Y77_000605"
     CDS             complement(15729..16361)
                     /locus_tag="AC1Y77_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574209.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16509..17051)
                     /locus_tag="AC1Y77_000606"
     CDS             complement(16509..17051)
                     /locus_tag="AC1Y77_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIQ1574210.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17186..17575
                     /locus_tag="AC1Y77_000607"
     CDS             17186..17575
                     /locus_tag="AC1Y77_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574211.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17579..18343
                     /locus_tag="AC1Y77_000608"
     CDS             17579..18343
                     /locus_tag="AC1Y77_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1574212.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18472..19419
                     /locus_tag="AC1Y77_000609"
     CDS             18472..19419
                     /locus_tag="AC1Y77_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574213.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19549..20289
                     /locus_tag="AC1Y77_000610"
     CDS             19549..20289
                     /locus_tag="AC1Y77_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574214.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(20580..21248)
                     /locus_tag="AC1Y77_000611"
     CDS             complement(20580..21248)
                     /locus_tag="AC1Y77_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1574215.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            21382..21669
                     /locus_tag="AC1Y77_000612"
     CDS             21382..21669
                     /locus_tag="AC1Y77_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574216.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            22085..23623
                     /locus_tag="AC1Y77_000613"
     CDS             22085..23623
                     /locus_tag="AC1Y77_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIQ1574217.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            23620..24585
                     /locus_tag="AC1Y77_000614"
     CDS             23620..24585
                     /locus_tag="AC1Y77_000614"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574218.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            24572..25894
                     /locus_tag="AC1Y77_000615"
     CDS             24572..25894
                     /locus_tag="AC1Y77_000615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIQ1574219.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      25957..26061
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            26135..26878
                     /locus_tag="AC1Y77_000616"
     CDS             26135..26878
                     /locus_tag="AC1Y77_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574220.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            26893..27885
                     /locus_tag="AC1Y77_000617"
     CDS             26893..27885
                     /locus_tag="AC1Y77_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574221.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            27897..28634
                     /locus_tag="AC1Y77_000618"
     CDS             27897..28634
                     /locus_tag="AC1Y77_000618"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574222.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            28624..29346
                     /locus_tag="AC1Y77_000619"
     CDS             28624..29346
                     /locus_tag="AC1Y77_000619"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIQ1574223.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT        10175 a         4998 c         6183 g         8064 t
ORIGIN      
        1 aaacatggtc gaatcaccat ttcattcaac cactatctgt gccgtcgaaa aagacgggaa
       61 atttgctatg gctggagatg gccaagtaac aatgggtgaa tctgttgtca tgaaaggcac
      121 tgcaaagaaa gtacgacgta tctacaatgg agaagttgtt gtaggtttcg ccggaagtgt
      181 tgcagatgcg tttactttag aagaaaaatt tgaaggcaaa ctaaacgaat ataacgggaa
      241 tctacaacgc gcagctgtcg aattagctca agaatggcgg acacagcaat ctatgcaaaa
      301 acttgaagcg atgctgatcg taatgaatga taaagaaatg cttcttgttt caggtacagg
      361 agaagttatc acaccagatg atggtatctt agctatcgga tcaggaggga attttgcgtt
      421 ggctgcagcc cgagcaatga aaaactatgc tcaaaacgac atgagtgcga aagaaattgc
      481 agaaaatgcg ttaaatattg ccgcagatat ctgtatcttt actaatcata atattatcgt
      541 agaagaactg taagaggtga gtagatatgt atgaattaaa acaaacacca agacaaatcg
      601 taagtgaatt agatgaatat atcgtaggac agcacgcagc aaaaaaatct gttgccgttg
      661 ctttgcgtaa ccgctatcgc cgtttgcagt tagatgaaaa aatgcaacaa gatattacac
      721 caaaaaatat gctgatgatc ggacctacag gggtcggtaa aacagaaatc gcacgacgtt
      781 tggcaaaaat cgtcaatgca ccatttgtca aagtcgaagc aacaaaattt actgaagtcg
      841 gttatgtcgg aagagatgta gaatcaatgg ttcgggattt agtagaaaat gcgatccaaa
      901 tcgtagaaaa agagcagtac agccgtgtat acacaagtgc taaaaaaaat gcagaaaaac
      961 gtttggtaaa aatcttggta cctggtatca aaaaagaaca gaaacaatcc actaatcaat
     1021 ttgaacaaat gatgaatctt tttaatgcta gtcagcaaaa taccgctgaa ccgcaagaag
     1081 aagtgacaga agatattcga atcaatcgcc gtacagtctt ggaacaacta caaaagggtt
     1141 tactggatag tcgtgaagta accattgaag ttgaagacca aaagaaacct gcaccaatga
     1201 tgaacaatgg attggagcaa atggggatcg acctaaatga aactctaggt gctttgacac
     1261 cgaagaaaaa agtggaacgg acggtaacag tcaaagaagc tttagagtta ttgatcaagg
     1321 aagaatcagc aaaattagtc aaggatgctg atatccatag tgaggcaatc aaattagcgg
     1381 aagaaagtgg aattattttt atcgatgaaa tcgataagat cacttctaaa tcacagcaat
     1441 ctggagaagt atccagagaa ggcgtccaaa gagacatatt gccaattgtc gaaggttcac
     1501 aagtaaacac aaaatacggg atcatccaaa cagatcacgt cctattcatt gcttcaggag
     1561 ctttccattt gtctaaacca agtgatctaa ttccagaact tcaaggacgc ttcccgatac
     1621 gggtagaatt agatgatttg acagctgaag attttgtgaa gattctgacc gaaccaaata
     1681 atgctttagt aaaacaatac gtcgcgttga tcggtacaga aaatgtgtca gttacgttta
     1741 caaaagaagc aattgaacgg atcgctcaaa tcgctttcga tgtcaaccgt gacacagata
     1801 atatcggcgc acgcagactg cataccattt tggaaaaact actagaagat ctattatttg
     1861 aagcaccaga tatgcaaatg ggagaaataa cgatcacaga agcatatgtg gatgacaaac
     1921 ttggctcaat tgcggcaaat gaagatttaa gtcgctatat tttataaaaa atcggaggac
     1981 gacaatgaca gacttgctaa cgaaaacccg caagataaac acacttttgc agcaaaagaa
     2041 tacttttgac atgcaagcag atttgcctta cgataaaatg gctgtcacac taggtggtat
     2101 tcttgacagc aacacctata tcataggaag tgacggagta ttactaggtt ataatgaacg
     2161 tcacgatgtg aacaatgaac gaatcaaaac aatgtttgtt gagaaacaat ttcctgaatc
     2221 ttatactgat acagtggcac agttgatgaa aactgaagct aatatcccaa ttactagtga
     2281 tattactgca tttccggtag agtcaagaga gaaatatcca tttggtctga cgactgtcgt
     2341 tccgatcttt ggagctggcg aacgtttggg tacgattatc ttatctcgta tggaacaatc
     2401 gtttgcagat gacgatctag tcttagcaga atatggagct acggttgtcg gtatgcagat
     2461 cctttatcag aagtctcgaa gcatcgaagc agatgtacgg agtgcaacgg ctgtccaaat
     2521 ggcaatcaac acattatctt atagcgaatt gaaggctgta caagctatct ttgaagcatt
     2581 ggatggagat gaaggtcgtc tgactgcttc taatattgcc gatagtatcg gcatcactcg
     2641 ttctgtgatc gtcaatgctt tacgaaaatt ggaatcagct ggaatcattg aatctcgttc
     2701 gttaggcatg aagggaactt atttaaaagt attgaattcc cgttttaaag acgagttagc
     2761 aaaacacagt tattagaagt aaggggactg gggcagatag aaatgtttgt tccagccttc
     2821 ccattttaaa aagtaagtcg aagttggagg aatggcaatg actgttatta ttcaaaatga
     2881 ccagctgatc gccgaaatcg ctgaacacgg tgcggaattg atcagtctga aatcaaaaga
     2941 acatggttta gagtatatct ggcaaggtga tccagcgtat tggggcagac atgcaccggt
     3001 tttattccct tttgttggac gtttaaaaga tgatcagtat gtttatcaag ggaaaacata
     3061 tcaaatgggg caacacggat ttgctcggga tatggatttt gaagtcattg aacatggaca
     3121 agaaatggct agtttcatct taaggagtac gccggagaca ttagaaaagt atccatttga
     3181 ttttgaatta gtgatcagct atgaattagg aggagacggc atcacggttc attatcaagt
     3241 ggcaaatacc ggtgaaaaag aaatgttctt ttctatcggt ggacatcctg ctttcaatgt
     3301 acctcttgaa gaaggattgt catttgaaga tttctatctg gcattttctc cattgaaatc
     3361 aagattacgc ctaccattga aaggcccatt catcgatatg gatcaaaaga cgttaggtca
     3421 gacaaatacg aatctggctt tgaatcgtga actttttgag caagatgcct tggtttttga
     3481 aacaaggggt ctaaatgcgt acgccattcg aagtgaaaaa tcttcccata gcgtaacttt
     3541 aagctataaa gatatgcctt atgtagggat ctggtctcct tatccaaaag aagcgccttt
     3601 cgtttgtatc gaaccgtggt gcggaatcgc tgacacggtg aacagtagtg gaaacttgat
     3661 ggaaaaagta gggatcaatc aactaggtgc ccatgaaatc ttcaagacaa aatattccat
     3721 cacagtgaag taagaggttg tgaccgagaa gttagcaggg aacaccgttt accaaggtgt
     3781 gtaaaaaagc tatcctgtaa gtaataaggg agtgtgacat cgatttgtca cactcccgtt
     3841 ttatctcttt ggaatgaaaa agtatcagga ttttttcttg ctgctattca aaccaaacgg
     3901 gattcgactt tcagtatggt ttctgatacg cttgatattg tctttatgcc gataaaagat
     3961 aaaggaagaa actaaaaagg caatgatcgt cagtaaccaa ttgggcttag ccaaaatcac
     4021 aggagctaca taaggaagga cgatcgtaga taaagtgatc aatactgcac tgatcatact
     4081 gctcaaactc accatacttg tacaaaataa actaacgata aagatgatac aagaataaac
     4141 aaaaaataca ggattaaatc ctaaaagcat tcctgcactt gtcgcaacag cttttcctcc
     4201 tttgaaccca gcaaaaatcg gaaatgtatg gccaagaatg gcgcataccc caaaccaaag
     4261 agggttaatc ccagaaaggt ggaagatcat cggcaaacaa gtagccaatg taccttttaa
     4321 aatatccata agcaggacaa caatcccggc tgttttgcct aatacacgaa atgtattggt
     4381 cgttccagta ttaccgctcc cgtgttgtcg gatatctttt ttgaaaaaca atttgccaat
     4441 ccagacaccg gaaggaatag aacctaaaag atatgctaaa ataagtaata agatcaattt
     4501 cattttttac cctctttcaa gttgaaaact atcctttatt ttagcatgaa taggtaacag
     4561 gaacaattga aaaaatgaaa gatacaagta taatagatgg aataaaaaga aaagcaaggg
     4621 gtggcagaaa tgtctgtaaa aaatccaaca caagaaacga ttttcaacta tttggaagaa
     4681 gcaaaaaata ttgccgtcgt aggcttgagc aataaaccag aacgaacaag ttacatggtt
     4741 gcacaggtat tgcaagacaa tggatataaa atcataccag taaatccatt attggctggt
     4801 gaagaaattc taggcgagaa agtctatgcg aatttgacag aaatcgatga tccaatcgat
     4861 atcgtagatg tatttagacg gagtgaattt ttacctgaaa tagcaggtga ttttttgaaa
     4921 actgatgcta aagtgttctg ggcacaatta gggattgaaa acaaagaagc agctgagatg
     4981 ctaagaaaag caggacgtac agatatcgta atggatcgtt gtattaagat tgaacttggt
     5041 aagttgaaag aaaaccaaaa ataaaaatga ccacaaagaa gagatgatcg atagagggga
     5101 atgtcgaaaa agcggttagg agataattaa aattgatttc tcgtctaccc tctttttttt
     5161 cacgaccttt tctagtatga taatcgtggt gcggttttgc cgcaaagtat tattgaagga
     5221 gcgtttgcct tggcaaaaaa agtaaacaac gaatacaatg actcgtccat ccaagtattg
     5281 gaaggattag aggcagttag aaaaagacca gggatgtaca ttggatccac tgacagccga
     5341 ggcttgcatc atttagtcta tgaaatcgtc gataatgcag ttgacgaagc attatcaggt
     5401 tatggcagtg agattgatgt gaccatccat gaagacaata gtataactgt tgcagatagc
     5461 ggacgtggga tgcctgtagg aatgcatgct tccggaatac caacagttga agtgattttt
     5521 actgtgctgc atgctggcgg aaagtttggt caaggagggt acaaaacttc tggtggactg
     5581 cacggtgtag gagccagtgt ggtaaatgcc ttatcgaaat ggctaattgt aacgatcgtt
     5641 agagatggcg tagagtatca gcagaaattt aaaaacggcg gtaaacctga cggtactttg
     5701 aaaaagatag gaaaaacaaa aaaagcaaac gggacgactg tccatttttt accagatgat
     5761 acaatattca gtacaacgaa attttcatat gaaattttag ccgaacggtt aagagaatct
     5821 gcctttcttc taaaaggcgt taaaatctct ctttctgatc ttcgtggaga agaaccagtc
     5881 aaggaaattt ttcattacga agaaggaatc aaagaatttg ttgattatct gaatgaagaa
     5941 aaagatacat tgacaccagt tgtttatttt tcaggagaaa aagaaggtat cgaagtagag
     6001 gtcgcttatc aatacaatga cggttattca gaaaatgttt tatcttttgt caataacgtt
     6061 cggacaaaag atgggggaac ccatgaagct ggtatgaaag cggcgatgac gaaatcttat
     6121 aatgaatatg cgagaaaagt tggtttgctg aaagaacggg acaagaatct ggaaggtagt
     6181 gatttcagag aagggttagc tgctgtactt tctatccgtg tacctgaaaa tcttctgcaa
     6241 tttgaagggc aaacaaaaga aaaacttggt acgccagttg ctcgtaccgt agtagataac
     6301 gtcatcagtg aacaaatggg cttttatttg caggaaaata gcgaaatgag ccagatgttg
     6361 gtccgtaaag caatcaaagc cagagaagcg agagaagcag cacgtaaagc cagagaagaa
     6421 agccgaaatg ggaagaagcg taaaaaaggc gaatctcttc tttcaggaaa attgacaccc
     6481 gcacaatccc gaaaccctaa gaaaaatgaa ttgtatttag tcgaagggga ttctgctggc
     6541 ggatctgtta aacaaggaag agacagaaag ttccaagcaa ttctaccttt acgaggaaaa
     6601 gtcatcaata ccgaaaaagc taaaatgcaa gatatcttga aaaatgaaga aatcaatacg
     6661 atgatctata cgattggtgc aggagtcggt cctgagtttt ctatcgaaga ctgtaattac
     6721 gacaaagtaa tcatcatgac cgatgcggat acagacggcg cccatattca agtattactg
     6781 cttactttct tttatcgtta tatgaaaccg ctgattgaag ctgggaaagt atatatcgcg
     6841 ttaccgcctt tatataaagt atccaaagga caaggaaaga agcaagtcat tgaatatgcg
     6901 tggacagatg acgaattggc agcaatgatt aaaaaagtcg gcaaaggata tatgctgcag
     6961 cgatataaag gacttggaga gatgaatgcg gaacaattgt gggaaacaac gatggatccg
     7021 acttcccgta cgttgatccg cgtccgtatc gatgatgctg cgcaagctga acgtcgagta
     7081 accacactga tgggtgacaa agtagaacct cgaagaaaat ggatcgaaaa tcacgtgcaa
     7141 tttacgctcg aagaagatgg cagtatctta gataaaaaag aggatactga aatctctcct
     7201 tcagtttcta atgatttatt agatgaagaa cgagcagata aaaatgaaaa caatcagtta
     7261 tttgaagtag aataggaggt cacctgatgg aaaatcgaca agaagtacaa gaattaactt
     7321 tagaagaagt gatgggtgac cgcttcggac gttattccaa atatattatc caagaacgtg
     7381 cactgcctga tatccgtgat ggattgaagc cagtgcaacg ccggatatta tttgccatga
     7441 acaaagatgg caatacgtat gacaaaggat tccgtaaatc agcaaaatct gtcggaaata
     7501 tcatggggaa ttatcatcct catggtgaca tcagtattta tgaagcgatg gtccgcatga
     7561 gccaagactg gaagttgcgg gaagtactga tcgaaatgca cgggaataac gggagtatgg
     7621 acggtgatcc acctgctgcg atgcgttata ctgaagccag attatccaag ttaagtggag
     7681 agatgctagc agatatcgag aaagaaacag tcgatctcgt gtggaatttt gatgatacag
     7741 aaaaagaacc aacagtcctt ccagctcgat acccaaacct tctcgtcaat ggatcgacag
     7801 gtatatctgc tggatatgca acagaaatcc cgactcataa cttagctgaa gtgatcgatg
     7861 gcacaatcta tatgatcgat catccacaag cgagccttga aaaattgatg gaatatattc
     7921 ctggacctga ctttccaaca ggagggatct tgcaaggaaa agatgaaatc aaaaaagctt
     7981 atgaaacagg acgaggaaaa gttattcttc gttcgaaaac aaagatagag gcaatgaagg
     8041 gtaataaaca gcaaatcgtg atcacagaaa tcccatatga agtaaataaa gcgaccttgg
     8101 tgaaaaaaat ggatgagatc cgtttgaata agaaaatcga tggaatagct gaagtacgag
     8161 atgaaagtga tagaacaggt ctgcaaattg tcgtcgaatt gaaaaaagac gccaatgctc
     8221 aaggaatttt gaattatttg ttcaaaaaca ctgagctaca gatcaattac aatttcaata
     8281 tggtcgctat cgaccatatg actccgcatc aagttggact gaaagacatt ttaagaagtt
     8341 atatcgagca tcgaaagcaa gtcataacaa aacgtagtca attcgattta gccaaagctc
     8401 aaaaaagaca gcatatcgta gaaggattga tgaaagcttt atcgattctg gatgaagtaa
     8461 tcgcaacgat tcgtgaaagt aaagacaaaa aagacgccaa aaagaattta gtcgacgtct
     8521 ttcaatttac agaagaacaa gcagaagcaa ttgtcacatt gcaattatac cgtttgacga
     8581 acacggatat cacagagctt caaaaggaat ccgaaagctt gattgcgcaa atcacagagt
     8641 tgaataaaat cctttcaaat gacaaagagc ttttctctgt tatgaaaaaa gaattgcgag
     8701 aagtgaaaaa aaattattct tccgctcgtc taacgacaat cgaagacgaa atcgaagaaa
     8761 tcaaaattga tacgcaagtc ttagtggcac aagaagacgt aattgtcagt gtgacgcgag
     8821 aaggctatgt gaagcgaaca agccttcgtt cttatagtgc ttccaaacca gaagaaatcg
     8881 ggatgcgaga aggcgactac ttgctatact caggtgaatt gagtacattg gaccatgttc
     8941 ttctgatcac aaataaagca aatgtgatct accgccctgt ccatgaacta cccgatttga
     9001 aatggaaaga tgctggtgag cacatttctc aaacaatctc caatctgtct gtagacgaat
     9061 cgattttagc tgttttccca tatcaaaaaa ttcaagcaga gaaaacattt gtattcatca
     9121 gcaagaatgg attgatcaag caaacacgaa tgacagagtt cgaaccatgg cgcacctata
     9181 agagccgtcc gctcagctgt atgaagctaa aggcggaaga agatgaactg attgctgtgt
     9241 atctgaaaga agatcagcca ggcttagatg ttttcttagt aacgcatcaa ggaatgggtt
     9301 tgcgctatcc tttaacagat gttcccgttg ttggagcaaa ggcagctgga gtgaagtcta
     9361 tgaatttgaa agatggagat aggattatca acgggctgct tgtgttagca gaaggtgata
     9421 cgccgatcgt aatcgttact cagcgaggag ctgttaaacg catgcttgct caagaaatca
     9481 gccaaacttc acgtgcgaag cggggagtga cggtcatgcg tgagctgaaa aaaaatccgc
     9541 accgtattat ctatatgtcg gaaggaagat caaaagaaat aaccttgatg aatcaaaaag
     9601 ggcagcagtt aacgattgac ccaactgatt tccctatagg tgatcgaaca tcgaatggat
     9661 catttgcgat ggatgagaaa aaaggtggag aagtcatcga agttatcgat gctcctgaga
     9721 ttttcatcga gtcgtaactg ttgtagtatt ttaatgaaga gacgccatct gtattaatac
     9781 aggtggcgtc tctttttcat tcatttttaa taaatcctta tacgtttgca aaaaaagatg
     9841 aaagtgttca caatgcttaa atgttggtat gtcaacgttg atttaaatat atgtaaataa
     9901 aacataaaaa ttgttaaata aaaaacaaga aaacccttgc aaagataggg tgctgtgtta
     9961 tactaatcac gtaaaaactg ttacataaaa cagaaattta ggaggtccat acaatggagc
    10021 aatggaatgg atttaaaggt accaaatgga aagaaggcgt agacactaga gactttattc
    10081 aaaataacta cactgaatat cgcggtgatg acagcttttt ggaaggccca acagaagcaa
    10141 cagaaaaatt atgggatgaa ttgcaaaagt taaatgatat ccaattcgag cgtaacggtg
    10201 tctatgatat ggacacagaa atcgtatcaa ctattacttc acacgatgca ggctatttgg
    10261 ataaagattt ggaaaaaatc gtcggtctac agacagacaa acctttgaaa caagcattca
    10321 tgccgttcgg cggaatcaat atggctaaca atgcgctaaa aagcaatggc tatacaccaa
    10381 acgatgaatt gacacatatt ttcactgact ggcgcaaaac acataatcaa ggtgtatttg
    10441 acgcttatac tgctgaaatg cgtgctgctc gtaaaaacaa aatcattaca gggcttccag
    10501 acgcttacgg tcgcggacgt atcatcggtg actatcgtcg tgtggcattg tatggaatcg
    10561 actatttgat ggaacaaaag aaaaaagatc atgatatgac tggttatcat acaatgagtg
    10621 aagaagtgat tcgtttacgt gaagaaatca ctgaacaata tcgtgcatta gcgcaaatga
    10681 aagaaatggc tgcgaaatat ggctatgata tttctaaacc agctgcaaat gcaaaagaag
    10741 ctgtccaatg gctatacttc ggatatttag cagcaatcaa atcacaaaat ggtgctgcga
    10801 tgtcaatcgg acgtatctca gcattccttg atatctatat ccaacgtgat ttggataatg
    10861 gagtaatcac agaaaaagaa gcacaagaat tgatcgacca cttagtaatg aaattacgta
    10921 tggtcaaatt cgcgcgtaca cctgaatata accaattatt ctcaggattc cctatctggg
    10981 ctactttatc cattgcaggt atgggattag atggtcgttc attagttaca aagaatgatt
    11041 tccgtctgct tcatacatta acaaatatgg gaccttcacc agaaccaaac ttgactgttt
    11101 tgtattctga acaccttcca gaaggattca gaacatatgc tagcagaatc gctatcgaaa
    11161 gttcttcgat tcaattcgaa aatgataatc agcttcgtga acaatggggt tctgatgatt
    11221 gtgcgattgc ttgctgtgta tcaggaacag ttgtcggaaa agacatgcaa ttctttggcg
    11281 ctcgtgctaa cttggctaaa acactattat atgccatcaa tggcggagtc gatgaagtga
    11341 ctggcgccca agttggacca gaatatcgtc caatcacatc agaaatacta gactttgaag
    11401 aagtgaaaca taacttcatg aatatgatgg attggttagc agaactatat gtcaatacgt
    11461 tgaacatcat tcattatatg catgataaat attcttatga agcagctcag ctggctttga
    11521 tggattctga cttgcaacgc acatttgcta caggtattgc tgggatctct catgcggcag
    11581 acagcttgtc agcgatcaaa tatgcaaaag taaaagcaat ccgtaatgaa aaaggaatag
    11641 cagttgattt tgaagtggat ggcgacttcc ctaaatacgg aaatgatgat gaccgtgcag
    11701 atgaattagt ttcatggatc ttggaatact tcatgggaca aatcaaacgt cataaaactt
    11761 accgcaactc aaaaccaaca acttcattat tgacgattac ttcaaatgtt gtttacggta
    11821 aaaatacagg aaatactcct gatggccgtg aagctggaaa accacttgcg ccaggagcaa
    11881 atccaagtta caatgcggaa caaagtgggt tattagctag cctgaactca acagctaaaa
    11941 tgaattacgg ctgtgcattg gatggaattt caaataccca aacaatcaat ccttcaggtc
    12001 ttggaaaaga acaagataca agaatcgaaa acttgcgtaa tgtcttagat ggatacttct
    12061 caaaaggagc ttatcatttg aatgtcaatg tattcagtaa tgatttattg cgtgatgcaa
    12121 tggaaaatcc aatgaaatat ccgaacttga cgatccgggt atctggttat gcagttaaat
    12181 tccgtgactt gacaagagaa caacaattag atgtcttgat gagaacttca cacgacagaa
    12241 tgtaaaaagt tcgtgaaaaa agtttttcga cttagcgtcg aaaggacgtt gaatagccat
    12301 cgcatcaaaa tcgaataatc agtatgctta tttgaaagaa acgatttaca ggggcaggac
    12361 ttgtatgttc cgtccctgta aattttacac ataacgaagc ggcaattgcg aaaaattttg
    12421 ttcagaaagc ttcgcttcga cagctaactt gtacttaaaa tgaaatcaaa gaaaagatgt
    12481 gaacttaaat ggaagaaaaa acaatcggct atgttcattc tatcgaaact ttcggttcag
    12541 tagacggacc tggactgcgc tttgtcgtgt ttatgcaagg atgccgcatg cgttgccaat
    12601 tttgtcacaa tccagatact tggaatattg gcggagggaa agaatacaca gcagatgaac
    12661 ttttagataa agcagaacgc ttcagacctt actggggaga taaaggtggt attactgtta
    12721 gtggtggaga acctctattg cagatcgatt ttttgatcga actatttaaa aaagcaaaag
    12781 agcggaagat gcatactaca ttggatacat gcggaaaacc ttttacgtat gaagaacctt
    12841 tcttttcacg ttttcaagaa ttgatgaagt atacagattt actgttattt gacatcaaac
    12901 acatcgataa tgaagagcat aaaaaactaa cgcattggga caatgaaaat attttagaaa
    12961 tggctcaata tttgtcaaaa atcaacaaac cggtttggat tcgccatgta ctagttcctg
    13021 agcgaagtga ttatgatgaa tatttgatcc gactagacaa ctttattcaa accttgtcaa
    13081 atgtagacag agtagagatt cttccttatc atacgatggg aaaatacaag tgggaaacac
    13141 ttggtttgaa atatccatta gaagggatcg aaccacctac aaaagaacgt gtcgaaaatg
    13201 ctaaacgatt gcttcataca gaagagtata caaaatattt aacgcgatag gtcgcatcgg
    13261 aaagaagata agtattgctt ctttccgttt ttttgctatt attaaatagg agaaatcaca
    13321 aaggagcgaa tgacatgtca aaaattttag tttttggtca ccaaaatcca gatacagatg
    13381 caatcggggc agccattgcg ttttcacact tgcaaaatga attaggtaaa gaaacagaag
    13441 cagttgctct aggtacacct aatgaagaaa cacaatacgc attagaccac tttcagctat
    13501 ctgcaccacg agtagtagag tcagctaaag cagaaaatgc agatgtcatg cttgtggatc
    13561 ataatgaatt ccaacaaagt atttcagata tcagcgaagt aaatatttta gctgtagtag
    13621 accaccatcg tattgcaaac tttgaaacag ctaatccatt gtactatcgt gcagaaccag
    13681 ttggatgtac aagtacgatt attttaaaac tatataaaga aaaccaagta acaattccta
    13741 aagaaatcgc aggaatcatg ctctcagcta tcatttcaga tacattgttg ttcaaatcac
    13801 caacatgtac gcctgaagat gaagcagctg caaaagaact tgcagcaatc gctgaagtcg
    13861 atctaaacac ttatggactt gaaatgttga aagcaggaac aaatctaagt gataaatcag
    13921 ctgcggttct tcttgatttg gatgcaaaaa gtttcccaat gggcagttcc aacgttcgta
    13981 tcgctcaagt caatacagtg gaccttaatg aagtgatgga tcgacaagca gagctagaag
    14041 cagcaatgga agatgaaatg aaagcaaatg gctacgatct gttcgtatta gttgtcacaa
    14101 acatcttaga cagtgattca gaattacttg ttgctggtaa aggaaaagaa aaagtagaag
    14161 aagctttcca aacaactttg accaacaacc gtgctttgct taaaggtgtc gtttcacgta
    14221 aaaaacaagt cgtacctcaa ttgacggctg tatttagcta atcaaatggt agtatgaagc
    14281 attcaaaatg aatggataag cgattgatat aaaaagaaac atagaacata ctaaaggccg
    14341 aagaaatgga ggagaataac agtcaattat gactcttgtt cttcctcctt tttacgtaaa
    14401 agccttttaa gtaaaataaa acggatagaa aagagaacat atatgaacta ttattcaaaa
    14461 aataaggaaa acttttatca aaaactgacc gatgatccgc ttttttcttc attgaccgat
    14521 tatctatatg aacatcgaga acaagaaacg attctaagag aattgaaaaa agaattttcg
    14581 caaaataaat tttcccattt tcttgattta ttgatcgatg caggattaat caagagggaa
    14641 gaacgaagat atcatttgaa ctttccaata tttgatccta aagattactt gcaacaagcc
    14701 acttcggctg ctgaaaccat agctgaacaa ttgaagagac tctctgttga tgaacaaaaa
    14761 ttagcgatgg gagaagttat ttgggcgtat tgtttcgaag atgaacggaa agaagcgtat
    14821 ttttatggag taagaaacag ccgagagaca gaattgctga ggacaactgc aggaaatgag
    14881 aagtaccgtt ttatcacact ttcctctata gaacacttcc cgcttacttt ggctaattac
    14941 ttttttgtac agaagaatca gcttcctgta acgaaggcat tcaaagaatt agccgaactt
    15001 attggagacg taaatgaatc gtactttttt gatcaaatag aagttatcgt tgacaggata
    15061 cgtaagaata aatataaaaa tcgccggcct agtattttcc atcagtctct acttgtgacg
    15121 aatactataa aggaagaaga atcattcaca ttagaattac ctattgtcga aaaaaacaat
    15181 tttgagattg aattaccaac attggatcct tcattgacaa tggaggaaat ggcctttctt
    15241 aaacgacaaa tcttttctga gctgagcaag aaatttatac cgcatgcatt ttcttatatc
    15301 aaagaatatg gaacagtttt agtgagtaaa acctaagaga gcagtaagca aaaattatac
    15361 tgactcttgc aggaggagcc tttctcttcc tttatggtta aaagaattat ttgagtattt
    15421 tgcctatcta ccacctatta gattcactac atggtctgga aacaaaggaa tgcaaaaaaa
    15481 tggtgagaaa tgtggaatga caaaggaagt agtgataaga aaagttagat ttttaaataa
    15541 tcaatattat gattcagtca agtatggcat tttatgggaa gaactagctg attagattag
    15601 gaagtccatt tgataatcca gaatcactaa gaaacgaatg gcgagtcatt aataatttat
    15661 ccaatcttgg gttattcttt tagattgaag tgtttaaaac taaaaaagca tttatgaagt
    15721 cgtaatagtc aggcttcaaa atgcttttta ttttgtttgt tttttctttg ccacataaat
    15781 acacctagac tgacgatgaa gagcatatcc aacagatttc ctgcgacttg gatcgtcgaa
    15841 gggttggtca gtggcattaa agcaaggaca aaaaaggaaa acaagctgta aataaaacag
    15901 gtgacaggaa ctaatcgaaa gaagagcgga catttcaatt ggctaaaaat gagtgaaaaa
    15961 ataaaataag taaaaatgga taaaatcacg actaaaccat tcatgataaa gaagaaaagc
    16021 gtattgtgat taaacgaata aaaaagaatt gctaaaaaag aaaacgtttt aaatgcaatc
    16081 aatacgcttg aagcttcgtt taacgtacca ggatgtcgat gaaaaaaata ggcaccgaaa
    16141 taaacagaaa acagtaagcc gcaaagagaa gacaaaatca aaaaaaacgg attttcttcg
    16201 gcttctgtga tatcagaatg atccaaaagc aaccagccta atgcactaga aggcgcatgt
    16261 gaggtcttaa ggtatgaata acatagaaaa aataagatcg tcaaagttgc aaaaaaagag
    16321 cctaaatgta aaaaattagt ggttctacgt tgaatttcca taatatctcc aaattaaatt
    16381 gataagtata taaagtataa taacaaattg atgagtatat gtcgatgttt tttaatcaaa
    16441 ataataaata aaaactgtct aacatttaaa atttaattta gatattagac agtttttaag
    16501 tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata
    16561 tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac
    16621 agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc
    16681 cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt
    16741 ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat
    16801 ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat
    16861 aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg
    16921 atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa
    16981 cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt
    17041 ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta
    17101 agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat
    17161 tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca
    17221 cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga
    17281 ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt
    17341 ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc
    17401 tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt
    17461 tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac
    17521 tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt
    17581 gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa
    17641 agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa
    17701 ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc
    17761 agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt
    17821 ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa
    17881 aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac
    17941 atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc
    18001 tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt
    18061 accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc
    18121 ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc
    18181 ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat
    18241 tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc
    18301 ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac
    18361 tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa
    18421 atttggtata attgatagta agaatgacaa catgttagaa aggaagagag catgcccact
    18481 ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat
    18541 catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg
    18601 gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa
    18661 ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga
    18721 atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc
    18781 gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg
    18841 atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat
    18901 gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat
    18961 tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa
    19021 actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca
    19081 aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa
    19141 acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat
    19201 gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac
    19261 caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta
    19321 tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt
    19381 ttttttcctg aactagttat cagagagaca acgaattaag tcttattact atatatatat
    19441 ttatgaaatt catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa
    19501 tctgtgctat tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc
    19561 agacgaaaac atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac
    19621 gacaagaagc gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc
    19681 ccgtttgaac agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc
    19741 aagcgtcgtg caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat
    19801 aaaggaaaat taccggatta taccggccat taccaatcag aaatcacatc gacaggacga
    19861 aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc
    19921 aagcatacta cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt
    19981 cctaaatatg tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat
    20041 catttaatcg aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg
    20101 acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa
    20161 acgttagagg aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta
    20221 gaacgctcat tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat
    20281 tttaaataag tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg
    20341 tttccttatt ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt
    20401 aagcgaattt tttctcccaa taagtaagaa aataatggca aaataaaaaa acaaactatt
    20461 ttcgcagatc gtttgcaaaa atagtttatt tttttataaa gaagatttta ctgaataata
    20521 tcattttagt ttacataatg atatttctgt aaattaaata aagtagtgaa aaataagaat
    20581 cagtgctcta actgttcaag aggatcaagt ggttcaaatg tttctgtagt ctgttttgct
    20641 tccaattggg gcaaaacaga tccaatttcc agatacttca ctttattcaa tgtataagat
    20701 agaatagaac agctaagtaa tgtatacaga aaatgtgtga gatccaaata aagcaaggag
    20761 acaactaaaa taccaaaagt aatcaagaaa agacttttgc caatcttaat atgaaaatat
    20821 ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc ttccgccgaa tcgaaagacg
    20881 agaccgcaac caatgccaga aagaatacct gtcatcaaac tattgatcag tagactatct
    20941 acagcaaaat gtatatgaag gctctcccat aaatgtaaaa agaaaactag cgcaaatgtt
    21001 ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa acaatgagat aagcaataaa
    21061 ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc caaaaagatt atgcaaaagc
    21121 aaagcaatac cagtaacccc accatctgcc agcttagatg gaatattgat tcctgtcaca
    21181 gctaatccgt agattcctgc gcctaaaatg attccgcaca attctgtaaa taaatttaat
    21241 tttttcattt ctagcctctt ttcttttgaa aattttcagc aacttcttta ttatactgac
    21301 ttatactctt acttcaagag gaaaatgaaa aaaaattatt tttggctgtt tctttttatt
    21361 tatcaaataa ggagtggaaa aatgcgagca gcagacttat tgacaattct tacaaaatca
    21421 cccaaaaaaa cagagtatca aggagttttt ttggctcaag aacaaaaact ggtgcctgtg
    21481 acaatggtcc agataaataa agcaaatcaa tttatctttt tatttgaaca cggcaaagca
    21541 ccacttgcta tgaaagacat ctttcttttt ttgatgaaga accgtgagaa agctgtttat
    21601 tatcagtcaa aagaagaact cattcctctt tatggagtga aagagataga aaataaagtg
    21661 gtgatataag taaaaataag aaaacaagac gtttatgatg tcaaaacaac cattttttac
    21721 atttttggca aaaaagtgtc atgatacagt ataatattcg ttgaacaggc caataaaggt
    21781 gttctccccc acgagatttc tactttattg gctgtttctt tttattaaaa taaaagttgt
    21841 gtgtgtaaaa aagatatagc tgattccaag tgacaatttc gtatagattt ttatggtatt
    21901 cgtagtgaaa aaacgagaac caaaaagttg atccgacttt gttgcttgga ttcttttttt
    21961 gtcagcatat ttagaggtat ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag
    22021 acaagttagt tgggaaaaag gaaagaaatg tttatactta aagaataaca gaaaggaagg
    22081 tcgcatgaat aattttatac aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc
    22141 atttcaacat ctgtccattt ctctcatttc tttgctgatt gcagcactga ttgctattcc
    22201 tttagcgatt tgggcagcca atcataaaaa aatggcagaa gtcttattgc agatcacggg
    22261 tgtgctccaa acgattcctt cacttgcctt attaggttta cttatcccat ttgtagggat
    22321 cggaactgtt ccagcattga ttgcacttgt tatttacgct ttgcttccga ttttccaaaa
    22381 cacgtatatt ggtctagctg aaattgaccc gtctattgaa gaagcagcgg tagcatttgg
    22441 tatgtccaga atgagacgct tgctgaaagt agagcttccc atcgcattac ctgtcattat
    22501 ctcaggtatc cgaactgcct tagttttaat catcggtacg gctactttgg cagcattgat
    22561 cggagcaggt ggattaggga catttatttt gttaggtatt gaccgaaata cacctgtttt
    22621 gacattgatt ggtgcgatca gttctgcctt gcttgcaatt atattcagcg gactgattcg
    22681 ctggttgcag cataagaaac ctcgatattc catcattact ttagcagcag tggtgtgttt
    22741 atttggcggc gtttctttat atcagtctgc cttgtttaaa aatgaaacca tcacgattgc
    22801 tggaaaatta ggagcggaac ctgaaatcct gatccaaatg tataaagaat tgattgaaga
    22861 caacacaaaa acagaagttg aattaaaacc gaattttggc aagacgagtt ttttgttcaa
    22921 tgcactggat caaggtcaaa tcgatatcta tcctgagttc acaggtactg tcctagaaag
    22981 cttggtgaag gtacctgaag caatgaaaag tgctgatttg acaaaagaaa aaacatatga
    23041 cgaagcagaa gaattattga ataagcaatt cgatatgaca ttattaaaac caatggctta
    23101 ccaaaatacg tatgccttgg cagtcaagag atcttttgcg aacgaacatc aacttaaaaa
    23161 gatttccgat ttggcagcta tctctgatca gatcaaagcg ggattcacac tggaatttat
    23221 tgatcgttca gatggatata aaggaatcca agaagcatat ggcttgaatt tttcaagtgt
    23281 acaaagcatg gagccaagtt tgcgttacca agcaatcaat aatggagatg tcaatattgt
    23341 ggatgcctat tccacagata gccagctcaa ggagtacgat ctagtgactc tagaagatga
    23401 tcgagggtta ttcccagctt atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga
    23461 acatcctgaa atcgttaaag cactgaataa attaagcgga aaaataacag aggatcaaat
    23521 gattgatatg aattaccaag tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat
    23581 cttcctgaaa aatgaaggat tagtaaagga ggaagatcag tgactaaatt aatcgagttc
    23641 aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa tctggcaatc
    23701 aaaaaaggag aaatctttgt tttggttggt ccttccggta gtggaaaaac aactactttg
    23761 aaaatgatca atggattatc taaaccatca gcaggagaca tctacttcaa aggaaaatcg
    23821 ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc
    23881 gctttgttcc cgaccatgac agtcaagcaa aatattgaag tcatccctga aatgttaggt
    23941 tgggaaaagc agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt tggcttatct
    24001 cctgatattt atcgagatcg tatgcctaga gagttgtctg gaggagaaca gcaacggatc
    24061 gggatcatcc gagctattgc tgcaagccca gatgtgattt tgatggacga gccatttagc
    24121 gcattagatc cgatttcaag aaactcatta caagaattgg ttttgtcctt acacgaagaa
    24181 ttgggcacga ccatcgtttt cgttacgcac aatatggaag aagcaatcaa gctaggtgat
    24241 cgtattgcat ttatgaagga tggagaaatt atccaatgtg acacaccaga gcagttattg
    24301 atgaatccta aaaacgacta cgttcggcat ttctttgatg aaccgaaaca gacgaaagag
    24361 tggcgagtag aagatttagt cgtcaatgga tatttcttaa atgaaatacc agaaaatgct
    24421 cgtcaacaag tttgctttga tacgcctatg aaagaagtat attcgcttct ttcggtgtat
    24481 ccaagcattg tgatagtaga aaagcaaaga agtattgggt cgctgacaag taaagaaatc
    24541 tttgcttttt taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg
    24601 ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa gaagcaggta aaacagtact
    24661 agtggtcgaa gaagatctat ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa
    24721 agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt
    24781 taacgagatc ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa catttacgga
    24841 tccagtccca gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc atctttcagg
    24901 aaccgctcgt tttttggatg attcatctat tgaagtcaac gaagaagtct tccatgcgga
    24961 ttacttagtt ttggcaaccg ggcaaagacc tacgatctta ccagtggagg gaaaggaata
    25021 cttgaaaact agtgcagact ttttatcatt gcctgtactg cctaaagaaa tcatttttat
    25081 cggtggtggt tatattgctt ttgagctagc tacgattgcc aatgctgcag ggagcaaagt
    25141 aaccattgtc catcataatc aacggccatt aaaagaattt gaggcgtctt tggtagagga
    25201 agccgtccat caaatggaag catctggcat tcagtttgct tttggtgttg aaacccaaaa
    25261 gatcatttca gaaggtactc gctatcgttt agtcggaaaa gagacggaat tagtagcaga
    25321 tatgattttt tgtgcgactg ggagacagcc gaatacagaa tctttagcac ttgagcaagc
    25381 gaatatcgtc tttgataaac acggaattgc agtcaatgat tatctgcaaa cgagcaatcc
    25441 aaaaattttt gcgtgtggag atattgtttc aaggaaaacc ccaaaattga caccagtcgc
    25501 cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg aacctatcaa
    25561 atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag taggagttac
    25621 aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca gttggttcac
    25681 ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc agcaaaatta
    25741 tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg atttgacact
    25801 cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg ggtatccgac
    25861 attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa agcgcttgtt
    25921 gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg gatgtcgtag
    25981 acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa tactggtgaa
    26041 gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt caagcgtttt
    26101 ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt atccagcagt
    26161 cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga aaatacctgc
    26221 ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg cacaagtatt
    26281 actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa tagctagcgt
    26341 tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa tgtcttttta
    26401 tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat tacttaccct
    26461 atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa gctattttcc
    26521 gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa tcatcttttt
    26581 gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac cagcgggttt
    26641 atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt
    26701 tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg taaaaaacag
    26761 ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga gtttattgct
    26821 gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata ttttttaaga
    26881 gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct tttatccgct
    26941 tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa aaaagtgaca
    27001 cttgttttgg attatgtacc gaatactaat catactggca tctacctagc aaaagaaaaa
    27061 ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga taattcgaca
    27121 agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca agaagatgtc
    27181 acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac agtgatcaaa
    27241 cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc aaaagatttt
    27301 gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg
    27361 atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg
    27421 ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg ggatatgatc
    27481 aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt agatgaacgg
    27541 ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag tgatccagaa
    27601 ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat taaggatcca
    27661 aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa
    27721 gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg gggattgatg
    27781 gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt gatcaagcaa
    27841 gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg atagatatgt
    27901 caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaaatatta gaagacatcc
    27961 atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga gccggtaagt
    28021 caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg atcaaagtag
    28081 acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa gaagatatgt
    28141 tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa
    28201 caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac ggctatgaac
    28261 attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg ttacgaacaa
    28321 tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta gatgctatga
    28381 ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag cggactactt
    28441 tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt cttgtgcttt
    28501 ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg
    28561 aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg gcaggtgaat
    28621 tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc actccttcag
    28681 caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac
    28741 agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc ttggaaagca
    28801 gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat catcggtgaa
    28861 atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa agtatttgag
    28921 cgacagcttg cttttgcagc cataaatgag aaacctgttg tactgcacac aaaaggatgc
    28981 gaaaaagaga tcttaaatta tatccaagct taccctaatc actatttgat ccattggtat
    29041 tcttctttgg actatcaaca agactatatt gatttaggct gttatttcag cattggtctt
    29101 gatttgaaaa agaatccagc agtctggcaa ctagctaaag cagtcccaat cgaccactta
    29161 cttattgaaa cggatggact acatgcagca gagtgggctt taggggggat tttttcagaa
    29221 aaagattatg tctcactgct tgaagaacag ttaaagacaa tcgctgagat caaagagcta
    29281 tcagttgaag aagttaaaaa aaatacttat caaaacttgg aacgtttcat tagattaggt
    29341 aaataaaaaa gagatgtgac acaactagtg tcgcacctct ttttggcttt ctttgtataa
    29401 aagtaaactg tttgattaat
//
LOCUS       DBNIBO010000015        28898 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.015, whole genome shotgun sequence.
ACCESSION   DBNIBO010000015 DBNIBO010000000
VERSION     DBNIBO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.015"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(5..586)
                     /locus_tag="AC1Y77_000620"
     CDS             complement(5..586)
                     /locus_tag="AC1Y77_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1574224.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="AC1Y77_000621"
     CDS             complement(769..1395)
                     /locus_tag="AC1Y77_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HIQ1574225.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="AC1Y77_000622"
     CDS             complement(1417..2748)
                     /locus_tag="AC1Y77_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HIQ1574226.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="AC1Y77_000623"
     CDS             complement(2903..3304)
                     /locus_tag="AC1Y77_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HIQ1574227.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="AC1Y77_000624"
     CDS             complement(3390..3596)
                     /locus_tag="AC1Y77_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574228.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="AC1Y77_000625"
     CDS             complement(3691..5193)
                     /locus_tag="AC1Y77_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIQ1574229.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="AC1Y77_000626"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="AC1Y77_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIQ1574230.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="AC1Y77_000627"
     CDS             complement(5764..6381)
                     /locus_tag="AC1Y77_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIQ1574231.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="AC1Y77_000628"
     CDS             6602..7975
                     /locus_tag="AC1Y77_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1574232.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="AC1Y77_000629"
     CDS             8050..9162
                     /locus_tag="AC1Y77_000629"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1574233.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="AC1Y77_000630"
     CDS             complement(9234..10871)
                     /locus_tag="AC1Y77_000630"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIQ1574234.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="AC1Y77_000631"
     CDS             complement(11039..11647)
                     /locus_tag="AC1Y77_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1574235.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="AC1Y77_000632"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="AC1Y77_000632"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIQ1574236.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="AC1Y77_000633"
     CDS             complement(13201..13953)
                     /locus_tag="AC1Y77_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574237.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="AC1Y77_000634"
     CDS             complement(14094..15044)
                     /locus_tag="AC1Y77_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574238.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="AC1Y77_000635"
     CDS             complement(15045..15884)
                     /locus_tag="AC1Y77_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIQ1574239.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="AC1Y77_000636"
     CDS             complement(15881..16627)
                     /locus_tag="AC1Y77_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574240.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="AC1Y77_000637"
     CDS             16865..18235
                     /locus_tag="AC1Y77_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1574241.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="AC1Y77_000638"
     CDS             complement(18290..19186)
                     /locus_tag="AC1Y77_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1574242.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19300..19470)
                     /locus_tag="AC1Y77_000639"
     CDS             complement(19300..19470)
                     /locus_tag="AC1Y77_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574243.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(19467..20144)
                     /locus_tag="AC1Y77_000640"
     CDS             complement(19467..20144)
                     /locus_tag="AC1Y77_000640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIQ1574244.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="AC1Y77_000641"
     CDS             complement(20235..21140)
                     /locus_tag="AC1Y77_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIQ1574245.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="AC1Y77_000642"
     CDS             21315..22769
                     /locus_tag="AC1Y77_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HIQ1574246.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="AC1Y77_000643"
     CDS             22785..23816
                     /locus_tag="AC1Y77_000643"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIQ1574247.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="AC1Y77_000644"
     CDS             23823..25010
                     /locus_tag="AC1Y77_000644"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIQ1574248.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="AC1Y77_000645"
     CDS             25166..26548
                     /locus_tag="AC1Y77_000645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIQ1574249.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="AC1Y77_000646"
     CDS             26520..26906
                     /locus_tag="AC1Y77_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574250.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="AC1Y77_000647"
     CDS             complement(27106..27774)
                     /locus_tag="AC1Y77_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIQ1574251.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27963..28760)
                     /locus_tag="AC1Y77_000648"
                     /pseudo
     CDS             complement(27963..28760)
                     /locus_tag="AC1Y77_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8040 a         5907 c         5299 g         9652 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcttgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc
    28021 tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc
    28081 tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc
    28141 tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac
    28201 tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac
    28261 gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc
    28321 gctaatgcat tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc
    28381 ataaaaggat ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg
    28441 tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga
    28501 cgatggatcc gatttttggc tactgctcca acttttcctc gataagaatt gtagcgacgt
    28561 gatttacgcg tgtatgccgt tacttttatt cctagcttct tgatcaagcg ctgaactttc
    28621 tttttattga tagagaaacc gcggtttctt agttcattgg tcatccgtaa acagccataa
    28681 tccttgtgct tctcccgaat tttaagcatt tccgcttcaa tctcttgatc tggattttta
    28741 cggtccaatc gcctttgcca tccaaataat catgaacgat ctttaattta agttcaaaag
    28801 tatatttaac cataaaaaat accgacctcc aaaagttaga ttttttggtc taacttttgg
    28861 gggtcggtac acaaatcgtg tcacagtccc ttttttga
//
LOCUS       DBNIBO010000016        28675 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.016, whole genome shotgun sequence.
ACCESSION   DBNIBO010000016 DBNIBO010000000
VERSION     DBNIBO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.016"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(198..1889)
                     /gene="argS"
                     /locus_tag="AC1Y77_000649"
     CDS             complement(198..1889)
                     /gene="argS"
                     /locus_tag="AC1Y77_000649"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIQ1574252.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     misc_binding    complement(1966..2163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2311..3258)
                     /gene="arcC"
                     /locus_tag="AC1Y77_000650"
     CDS             complement(2311..3258)
                     /gene="arcC"
                     /locus_tag="AC1Y77_000650"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIQ1574253.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(3373..4392)
                     /gene="argF"
                     /locus_tag="AC1Y77_000651"
     CDS             complement(3373..4392)
                     /gene="argF"
                     /locus_tag="AC1Y77_000651"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIQ1574254.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(4483..5712)
                     /gene="arcA"
                     /locus_tag="AC1Y77_000652"
     CDS             complement(4483..5712)
                     /gene="arcA"
                     /locus_tag="AC1Y77_000652"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIQ1574255.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            6173..6874
                     /locus_tag="AC1Y77_000653"
     CDS             6173..6874
                     /locus_tag="AC1Y77_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIQ1574256.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(7446..8462)
                     /gene="tsaD"
                     /locus_tag="AC1Y77_000654"
     CDS             complement(7446..8462)
                     /gene="tsaD"
                     /locus_tag="AC1Y77_000654"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIQ1574257.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(8459..8923)
                     /gene="rimI"
                     /locus_tag="AC1Y77_000655"
     CDS             complement(8459..8923)
                     /gene="rimI"
                     /locus_tag="AC1Y77_000655"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIQ1574258.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(8930..9472)
                     /gene="rimI"
                     /locus_tag="AC1Y77_000656"
     CDS             complement(8930..9472)
                     /gene="rimI"
                     /locus_tag="AC1Y77_000656"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIQ1574259.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(9456..10178)
                     /gene="tsaB"
                     /locus_tag="AC1Y77_000657"
     CDS             complement(9456..10178)
                     /gene="tsaB"
                     /locus_tag="AC1Y77_000657"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIQ1574260.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(10369..11349)
                     /locus_tag="AC1Y77_000658"
     CDS             complement(10369..11349)
                     /locus_tag="AC1Y77_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574261.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(11373..12857)
                     /gene="galT"
                     /locus_tag="AC1Y77_000659"
     CDS             complement(11373..12857)
                     /gene="galT"
                     /locus_tag="AC1Y77_000659"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIQ1574262.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(12869..13858)
                     /gene="galE"
                     /locus_tag="AC1Y77_000660"
     CDS             complement(12869..13858)
                     /gene="galE"
                     /locus_tag="AC1Y77_000660"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIQ1574263.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            14107..14274
                     /locus_tag="AC1Y77_000661"
     CDS             14107..14274
                     /locus_tag="AC1Y77_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HIQ1574264.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(14336..16147)
                     /locus_tag="AC1Y77_000662"
     CDS             complement(14336..16147)
                     /locus_tag="AC1Y77_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIQ1574265.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(16144..16509)
                     /locus_tag="AC1Y77_000663"
     CDS             complement(16144..16509)
                     /locus_tag="AC1Y77_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIQ1574266.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(16672..17067)
                     /locus_tag="AC1Y77_000664"
     CDS             complement(16672..17067)
                     /locus_tag="AC1Y77_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIQ1574267.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(17085..18047)
                     /locus_tag="AC1Y77_000665"
     CDS             complement(17085..18047)
                     /locus_tag="AC1Y77_000665"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIQ1574268.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(18047..18259)
                     /locus_tag="AC1Y77_000666"
     CDS             complement(18047..18259)
                     /locus_tag="AC1Y77_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HIQ1574269.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(18280..18978)
                     /locus_tag="AC1Y77_000667"
     CDS             complement(18280..18978)
                     /locus_tag="AC1Y77_000667"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIQ1574270.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(18998..19540)
                     /locus_tag="AC1Y77_000668"
     CDS             complement(18998..19540)
                     /locus_tag="AC1Y77_000668"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIQ1574271.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(19672..20676)
                     /locus_tag="AC1Y77_000669"
     CDS             complement(19672..20676)
                     /locus_tag="AC1Y77_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIQ1574272.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(20673..21662)
                     /locus_tag="AC1Y77_000670"
     CDS             complement(20673..21662)
                     /locus_tag="AC1Y77_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIQ1574273.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(21659..22465)
                     /locus_tag="AC1Y77_000671"
     CDS             complement(21659..22465)
                     /locus_tag="AC1Y77_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574274.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            22631..23587
                     /locus_tag="AC1Y77_000672"
     CDS             22631..23587
                     /locus_tag="AC1Y77_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574275.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(23664..24182)
                     /locus_tag="AC1Y77_000673"
     CDS             complement(23664..24182)
                     /locus_tag="AC1Y77_000673"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIQ1574276.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(24270..24434)
                     /gene="rpmG"
                     /locus_tag="AC1Y77_000674"
     CDS             complement(24270..24434)
                     /gene="rpmG"
                     /locus_tag="AC1Y77_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIQ1574277.1"
                     /translation="MGGKNMRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRE
                     RKVTLHRETK"
     gene            24647..25093
                     /locus_tag="AC1Y77_000675"
     CDS             24647..25093
                     /locus_tag="AC1Y77_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIQ1574278.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(25288..27183)
                     /gene="asnB"
                     /locus_tag="AC1Y77_000676"
     CDS             complement(25288..27183)
                     /gene="asnB"
                     /locus_tag="AC1Y77_000676"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIQ1574279.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(27508..28482)
                     /gene="bsh"
                     /locus_tag="AC1Y77_000677"
     CDS             complement(27508..28482)
                     /gene="bsh"
                     /locus_tag="AC1Y77_000677"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIQ1574280.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         8477 a         6045 c         4908 g         9245 t
ORIGIN      
        1 gggagctgtg acacgacttt tgtcacagtc cccttattta caaacaaaca gtgtttaaga
       61 gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc tcaaaaaacg
      121 ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca gaacgctgat
      181 ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa acgaaggtct
      241 tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt ttgttcatca
      301 tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa tttgattgca
      361 tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt ttcagggtat
      421 ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata gtctgctgtt
      481 tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg tgtatattgc
      541 acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa gtcaaatgta
      601 ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac ttgtttagct
      661 acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt agcagattgg
      721 attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt tgataatttt
      781 ttaccacctt gcgtgatcaa accaaatgga atatggcgca tatcgtcaga ccagtcaaag
      841 cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc attccctact
      901 acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc agctaagtca
      961 cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt caggtcataa
     1021 gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt ttcttctaaa
     1081 agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa cgaatcgaaa
     1141 cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc acggaaccat
     1201 tgccataatt gaatagcctc ttcatcgcct tcttccagac gtttgaacca agcacgagct
     1261 tcttcattta attctggttc tgtttcagct acttcatgga attggacata taaacgaagc
     1321 aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt gtaagcgacg
     1381 atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat tggctgataa
     1441 ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg caaatgtccc
     1501 attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt tccttgattc
     1561 ccgatattgc tatccccata atgttctttt tctttcacta ctgtttgcaa aacttttttg
     1621 ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc gattttttca
     1681 aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg cgcttttcgg
     1741 taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc tgattttgga
     1801 ttttctagta attgttcgac ttgttccagt gttaaatcat ctttgacagc gtcatagaca
     1861 gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgct cccttgattg
     1921 ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag agacgagatt
     1981 tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat atcggtagac
     2041 gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct tcattcggct
     2101 tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt tcaacaaatc
     2161 ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc tagtcgtttc
     2221 tgataaaaaa acaatccaga tggcctaaag gagaagaaat accacctgga tagctataaa
     2281 tcatttcgta aatacgaaag acttaacaaa ttaatcagct acgataatcg ttccttcttc
     2341 agaagctaaa agattctcga tattttctaa agaagtgatg attgccttag cgtttggttt
     2401 tgcttcaaca aatgcaatag ctgcctctac tttcggaagc atgcttcctg gagcaaattg
     2461 cttttcatca atgtattgtt tcatttctga aacagtaaca gtttctaatt tcttttgatc
     2521 tggtttttgg taattgacat acacattgtc tactcctgtc aatacgatca gcaaatccgc
     2581 atcgatgatt tcggctaatt tttctgaagc aaaatcttta tcgattacgg cttctcgtcc
     2641 ttctaatcca gtagctgttt ctacaacagg aatcccaccg ccaccaacag agactgtgat
     2701 aactccttga tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga tgggtttagg
     2761 ggaagcgact acttttctcc atccacgtcc agcatcttct ttgaatgtca cagtggagtc
     2821 agcattcatt tgttctttgg cttcttcttc cgtgtagaat ggaccgactg gtttacttgg
     2881 atttttgaat gaaggatcat tttcatccac aataacttga gtgactaaag aaaccacatc
     2941 tttatcgatc cctttttcct tcagtacttc gcccatggca ttttgtaacc agtaaccgat
     3001 agatccttct gtcattgcaa cacatgtatc taaaggcatt gccggtgttt tctcagaatc
     3061 agcagcttgc tgctgaatca ataggttacc gacttgcgga ccatttccgt gggagatgat
     3121 caattcatct ccttgttcga taaatttcac taaatacttt gctgtttcca ttaacgcttc
     3181 ttgttgggct tttgcacttg catccgtaga caagattgca ttacccccta aagcgacaac
     3241 tacttttcgt ttggccattt cattcgtcct ttcttcagtt ctaccgaatt ttctagataa
     3301 caaaacgagc cagcgttttg tgattttccc tcaatgctga ctcgtttttt cgtgagatga
     3361 atgtggttat gcttaaacgc gagggataaa caagttacct aatgttgctg ccatgatcgc
     3421 tttgattgag tgcatacgat tttctgcttg gtcaaattga cgagcatatt tgctgcggaa
     3481 gacttcatct gttacttcca tttcagtgat gccaaagcgt tctttcattt gttcaccgta
     3541 aacagtgttt gtatcatgga aagctggcaa gcagtgcaag aagatcaaac gatctgtgtt
     3601 gtgtgttttt tctaccattt ccatattgat ttggtaaggt ttcaacaatt tgatacgttc
     3661 ttcgaattta tcttcttcac ccattgatac ccatacatca gaataaagga cgtctgctcc
     3721 gtctacacct ttatcaacat cgtcggtaat catcaattgt gcaccggatt tttcagcaaa
     3781 gccttctgcc attttgacga tttcttcttc aggttgtaat tcttttggtg caacgatacg
     3841 catattcgct cctaaaattg cacctgtgac taataaagag ttagccatat tgtttcgacc
     3901 atctccgcag taagcaacag tgataccttc tactgttcca aagttttctt ggatagttaa
     3961 aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc aagccattcc aaactggaac
     4021 accagagaat tctgctagtt cctctaccat tttttgactg aatccacgga attcgattcc
     4081 atcaaacata cgacctagta ctttagctgt atcttctgtg gattcttttt tacctaattg
     4141 gatatcattt gcacctagat attcaggatg tgcacctaga tcgatcgctg cagtagtaaa
     4201 tgcagaacga gtacgtgtag atgttttttc aaataaaagc gcaatatttt ttccttctag
     4261 atagtgatgt gggataccac gttttttcaa gtctttcaag tgttctgaaa aatcgatcaa
     4321 atattgtaat tcttcttttg taaagtcttt ttctgctaat aagcttcttc cttggaatac
     4381 tgattctttc atattaaaca ttctccttta gattgttttt atttaagatt aaagaggaaa
     4441 tcccgacggc agctgacagt aagaccgccg tcaggaaaca aattacttca agtcttcacg
     4501 gacaagtggt tggctcatgc agcgtggacc accacggcct cgtgacaatt cacttgaatt
     4561 gatttctaac actttgatgc catagctacg aagcaattcg ttcgatacat aatttcggtt
     4621 ataagtgact acaacacctg gagcgattgc caatgtgttt gatccatcat tccattgttc
     4681 tcgaggtgca acgatctcat ctccattacc agttgggatc agtacaagat catctaatcc
     4741 aagagcagct ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga tatcatcacc
     4801 atctggttcg attgtaaaga catccatctt accgtcttta ctttgaattg ctggatggat
     4861 agtgaattta tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca ttgcacggtt
     4921 gtttggaatt ttgattgcta acactttttc aaatcctgaa ttttttgcga ataggtggcg
     4981 tgctaatttt tctaaagctt tcgcattagt tcgttgagaa atcccaacag ctactacttt
     5041 atcgcttaag atcaattcgt ctccgccttc gatatgatct ggattttcac gatccaacca
     5101 tacttcgatt cctttatttg caaatcttgg atgatgtttc aaaatatatt ctgtaaacat
     5161 cgattcacga cgacgtgctt caaaagtcat cttattgact gtcatcccat ttcccattga
     5221 tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa atggatagtc
     5281 atcgtctgct gacagatcgt atagactgct agaacgtact tcaatatctt ttgtacgaac
     5341 cccagccatg attttatcta ccatttcttg cgtttccatt gatagaagga actcatgaag
     5401 accctctctt acagcattcg atgcaatatg agattcatcc aacattttat tcaagaattg
     5461 ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt aaagagtctc
     5521 gacgccttca ttttgcaatg tctttgcaaa gttgtcatgt tcttcttgag caatcggaag
     5581 atatggaata tcatcgaaca acagacgatc cataatatct ggtgtcaaat tttccacttc
     5641 ttgtccaggc cttttcagta aaactgtctt taatttccct atttcagaga aaacgtgaat
     5701 aggtttatcc atgttgtgtt gcctccttct ttttgattac atgtatagga tatcctcctt
     5761 ttgtaatcac tttcataatt ttgtctcatt caaaatgaga cattcctcac attaatattc
     5821 gataattaaa taattaaacg tttttctatt tttaagcgta atttattcat tttttattct
     5881 gtatacactt tcaaaaaaat atttattcat tgaaaaaaat tttttgaata aattaaaaaa
     5941 ataaattata ttagcgttgt tagcgtttac tatatgtatt attgttgttt tttatacaca
     6001 aatcattcgc ttcaaaacaa attattcaat accattgtat attttttaca taataacaaa
     6061 ttatatttgt aaagtattaa aacttgtttc taatgttggt atgttaaaac tttacaaatt
     6121 ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt
     6181 taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc
     6241 ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca
     6301 gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt
     6361 tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa
     6421 taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc
     6481 gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa
     6541 aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga
     6601 attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt
     6661 cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc
     6721 gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt
     6781 aatcaaacaa ctacgtgatt ccggactgtt agattatgac aaaaagcgtt ttactttctg
     6841 taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct
     6901 gatatttatc agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt
     6961 ttagatcact gggcaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac
     7021 tgtttgcaaa aaatactgat agattaacta gaggaagcgt cttgtcctca tccgacctag
     7081 attattcgat attcctcgac aataaacgat taatcagtga gggcaatcag aggaacggat
     7141 aatgatgaat aagctgtaaa aaatagggct ttgacaaaag cgtgtcacag cccttatttc
     7201 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
     7261 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
     7321 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
     7381 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcaga tttttcacaa
     7441 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
     7501 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
     7561 cgaagagggg gaatcactag ttctacctca gggagtttag ttgccaaagc atcctgcaat
     7621 ccttcacgca acccttgatt ggccgctaca cctccagcta caatcagttg cttgactgga
     7681 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
     7741 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
     7801 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
     7861 gctcgtggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
     7921 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
     7981 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
     8041 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
     8101 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
     8161 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
     8221 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
     8281 cataaggtga tttgttctac gtgatggcgg ctagctactt caggaactac gccaccaaaa
     8341 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
     8401 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
     8461 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
     8521 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
     8581 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
     8641 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
     8701 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
     8761 ggttatcttc aactaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
     8821 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
     8881 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
     8941 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
     9001 tcgttgttgc ttgaaaacct aacttccgat aaagtctttg tgcatcttga ttactcatac
     9061 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
     9121 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
     9181 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
     9241 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
     9301 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
     9361 tagtcaattc tcttagtacg taacgacgat cgatttgttc gattgttttt tctgtataaa
     9421 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
     9481 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctacccgttt taaatagcga
     9541 ggaagaaaag cttgtttctc tgcaactggt tcagctaaag cacctaattg agccacagtt
     9601 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
     9661 tttatttcat agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
     9721 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacaatttct
     9781 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
     9841 atgggcacaa taattttatc cacaccgatg caattagccg cgatggtttt cagactagat
     9901 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
     9961 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
    10021 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
    10081 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
    10141 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
    10201 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
    10261 aaactagtac aatgatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
    10321 acaaaaaagc tgcggtatca cttcatttcc ccacagcttt tccatctttt atttcgttga
    10381 ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt
    10441 acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac
    10501 agtagaaaga gagggcgtta catatttcgc tacactgaca tcattgaaac ccatcacaga
    10561 aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa
    10621 tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatactgctt
    10681 cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta
    10741 gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg
    10801 gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac
    10861 aaaatgttca atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc
    10921 ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa
    10981 taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgtcggaa gatcactaat
    11041 ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc
    11101 tagacgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat
    11161 caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg
    11221 ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata
    11281 gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat
    11341 tgttgccatt tgtctctcct ccttttttac gtttataaaa ttgcagtaaa tcgttcaaac
    11401 gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa
    11461 acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt
    11521 tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct
    11581 aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc
    11641 cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga
    11701 tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac
    11761 tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct
    11821 gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccactt ttccaatact
    11881 gagttagctg cttcaatcaa caattttttc tctgttgatt gcaaacggat cacagacatc
    11941 ggccatttca cgactcctgc gatgacttgc ggataatctg ctagtttgat aagtttttct
    12001 atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga
    12061 atcgatcctc caacaatcgg caagtcagca ttcgaaccaa caaagtaatg aggcaagact
    12121 tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt
    12181 tctgataaaa tgatcgaatg ttcattgtaa taagcataag gagaatattg gaatccccag
    12241 ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag
    12301 cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc
    12361 gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg
    12421 atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt
    12481 gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg
    12541 cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa
    12601 tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt
    12661 tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt
    12721 acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat
    12781 aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc
    12841 gtttgactga ttgacatatc tcattcccct attcttcata accatttgga tggctgacat
    12901 gccaatccca tgctgttttg ataattgctt tgatatctgt gtattcaggt ttccaaccca
    12961 gaacctctct cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg
    13021 gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct
    13081 tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca
    13141 agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga
    13201 tacatgttcc atctggtgta tcataatcgt ctccataaac agccaatgct tttctttgac
    13261 caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga
    13321 tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc
    13381 catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg
    13441 gatttttagg attagctggt gtatcttctg tgattggaga ctcttttggt tctccgtaag
    13501 ttgctgcagt agaagagaac acgattttat tcacattgaa ctcgtgcatg acttcaagta
    13561 agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc
    13621 ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg
    13681 aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg
    13741 cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta
    13801 gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa
    13861 aaaacactcc tttgattagt aaaattgtgt ttgttgatta tatataattt aaaaaaacga
    13921 agtttacttt tatggtgaaa acaaacaaag taaaatttcg taaattcata tggtaaattt
    13981 ataataaaac atttagtaaa cattttcaag gattttttgc aaaggtttac aaacaaacgt
    14041 aaaactagcc tttttttctc ttttgttcca taatagatat aacaaataat aaatgaggtg
    14101 gattcaatga aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc
    14161 gttgctggtt tggtcgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa
    14221 gcaatgatcg aagaaaaccg taaaaaagca atgcgcaaac gcgtttctcg ttaataatta
    14281 tttaaaaaca cgcatgaaga cttgtgaaag aacagataag tcatcatgcg tatttttatt
    14341 tgtcagtcaa tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat
    14401 aaggtctagc tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat
    14461 aaaagtcaga ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat
    14521 ctatagcttc tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt
    14581 tgttttcctt caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca
    14641 atcgagcttc aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc
    14701 ggttgtgctt ttgctgaata tcaaatggcg gttcgaacaa atgaaaaata cccgcatcta
    14761 tcagtgctgc taattctttc gtccgaatga ctggcggacc gatcgttaaa aagctattta
    14821 gatgagtaaa ccatccccat agtatttctt tgtattcctt tgcagaaaaa acgtgccatt
    14881 caatagcctg ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg
    14941 ctcctgtgca attgcctttt tccgcttctt ttatctgccc ttcaataaat gctcggctag
    15001 cttctgcaaa ggactcatcc tgataggtaa gaggaatttc taaatacgac caatcccatc
    15061 tataagggat tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat
    15121 catgctcaaa atttttcttt ggaatcggca aatgatattc ttcgatgatt ttcttgtaat
    15181 aaaacaactc aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg
    15241 actgatgcca ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgatccgc
    15301 cttgcttttg gtttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata
    15361 caatcggttc cttgcctgat ggatgataga caagttttcc tttttcgttc tcgaagcgcc
    15421 cttctcttga tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta
    15481 aaaagacatt ttctttaggc ggtatcttcg aaagatcgac atctgcagga ttagaaggaa
    15541 gctgatacag taaatcattt ttctttgcgt agtctaccca cactctttct tcttctgtct
    15601 catcattttc ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat
    15661 ctgtctgtaa aagataacca tcttttcttt tgctgacttt ttttactaaa gaataaacaa
    15721 actgaataga agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc
    15781 cgtataaaca tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta
    15841 gttgcttatt tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt
    15901 gaaacaagtt gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa
    15961 gcgtcacttg ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa
    16021 ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat
    16081 tattatggag aatcctttct gctatactta atcctctagg tccggcacca atgatcccta
    16141 ttttcatttg catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg
    16201 ttcatttttt gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag
    16261 tttaaaaata gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc
    16321 aaatgaagca aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc
    16381 aatttctttc aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa
    16441 cctatccacg agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa
    16501 tttgatcata gttgtttcct tctttctgcc ttttcgctta gcttatatat gtagataata
    16561 gcacaaaata aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt
    16621 caaaatctaa tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt
    16681 tttacttttg atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg
    16741 ataacctgct ttacgtaatt tatttgctgt acggatgctc aagcttttct tttgatcgta
    16801 aatatagaca ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg
    16861 gatgtttctt gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat
    16921 tacttgtgct tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg
    16981 cttggccatt actcgtagat agacttccca aaagatgatt gcaagcacaa tcaatagcaa
    17041 aatgatatta attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag
    17101 gctaatgagg cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt
    17161 gttgattctc ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt
    17221 aaaaattctc ctgctgccga tactcctcca cctaacacaa tacttgacgg attcaatgta
    17281 ttccccaagt tggcgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat
    17341 gcaaacggat cgtcttcttg agccatttca aagatatctt tacttgttac ttcttcaccg
    17401 ttgtctaaca ttgctttcag ttttgaatct cctgcgtatt cttctgccaa gtaacgacct
    17461 agacgtacca caccagtagc gctagacaca gtttctaaac atccgcgttt accgcaagta
    17521 cattcgaacc cttcaggatc cacagtgata tggccgatct ctccgcctgc tccgctcacg
    17581 ccgtgaatca aatttccgcc agcaacgata ccaccgccaa cacctgttcc tagagtaacg
    17641 aatacaacgt ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca
    17701 ttcgcatcat tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca
    17761 aattgaactt ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc
    17821 acgcttcctg gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa
    17881 ttcaaacgat ggttgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa
    17941 atattggtat cgatgctcca tttttgttga atctctcctt ctggtgttaa aattgcaaat
    18001 tttgctgttg ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt
    18061 tctttcggct ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg
    18121 tatcatgatc aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa
    18181 tgtcatagat gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta
    18241 catcatataa gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt
    18301 ttttaaaccc agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat
    18361 cctttggtgt atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac
    18421 taatccgcca attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc
    18481 aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg
    18541 gttatgttta aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt
    18601 actcgcacca ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat
    18661 tatcccgctg aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg
    18721 ctgccccata aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat
    18781 gaagattggt gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat
    18841 tccggcataa taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt
    18901 ctgtatccct aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat
    18961 ttgctgttga taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga
    19021 tcttctgatc aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc
    19081 tacatgtttt tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga
    19141 ttcgataacc ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg
    19201 gcaaatagag cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg
    19261 actgttcatc catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta
    19321 ttttcttttg ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat
    19381 tcgataaagt cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt
    19441 tatagattcg ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt
    19501 cgattccttt ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct
    19561 cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa
    19621 tagaataaaa agaagaccta gaaatgattg gccttccttt tttaataaat ttcaactgtt
    19681 cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc
    19741 aatcaaaatt cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc
    19801 actaagcaga aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt
    19861 acgtacgact agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt
    19921 caccgctaca ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt
    19981 agccacgtta aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atccaatcct
    20041 ctgtgcataa agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac
    20101 ataaggccag ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt
    20161 ttccttagat aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc
    20221 aataccggac aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag
    20281 atagacacag caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg
    20341 cagcgaatag tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt
    20401 aatgcccaat atactagaat ctgccaattc atttcttgtt actccttgca agatataacc
    20461 agaaacgcca agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc
    20521 tctaacaatc agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc
    20581 caggtaaata aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa
    20641 aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc
    20701 agtaaaaacg gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga
    20761 ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag
    20821 acaggtagaa cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac
    20881 agcccaataa atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact
    20941 agacagaaaa caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt
    21001 tcactcaaat gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt
    21061 atatataaag gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca
    21121 aaccaaaaag caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca
    21181 gtaaaaaaag aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt
    21241 ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac
    21301 attagaaaca cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc
    21361 aacgaagcac ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcact
    21421 ccttgcatca atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca
    21481 cgaggcaaac gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac
    21541 aacgcctgcc aaaaatctgc ttttcccaca gtcgctgcgc catttgaaag agcaacaaaa
    21601 aaaataatca aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc
    21661 ataattgatc ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg
    21721 ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg
    21781 tgatcacttc ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat
    21841 aatctgaaaa acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt
    21901 tcatttgatt gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa
    21961 atgttgtcgg ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc
    22021 agacacgctg ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta
    22081 gacctgtagc atccaacgcc cactcgattg cttcatgatc ttgcgtgtcc atgccactga
    22141 acagtttttg ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag
    22201 ggtgttcact agtttgtgct aataatgtaa ttttttttgc aagttctttt gaactgtatg
    22261 tagagatatc agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa
    22321 aacttttcaa taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc
    22381 cttcaggaat cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa
    22441 tgtcagctgc tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt
    22501 atgacgctgt ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg
    22561 ttgacagcgt tcgaaatatc aggaataata accaatgaga aagattctca attatgttag
    22621 gaggaaagaa atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc
    22681 tggagtactg agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc
    22741 gacgacttca tccaatagta ctcatacagt cacagataca cttggacata aagtagagat
    22801 tccaaacgcg cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg
    22861 ggaaaaacct atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga
    22921 tgatttaaag gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt
    22981 tgagcctgat ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca
    23041 atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga
    23101 acaactaaaa gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc
    23161 cgattatcaa aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa
    23221 gacagctgca gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc
    23281 aagtggaaga atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt
    23341 ctccaaaaaa gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga
    23401 tgctgactac atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag
    23461 ctggaagaat cttaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag
    23521 ttggctatat aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact
    23581 aaattgattt taactaaaaa aagaatcttc acgattagtc gtgagatgct ttttttctta
    23641 ttaacaaact agacgcatag attttattct tcttttgaag caaccctatt ttttactctt
    23701 tcccattttt gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc
    23761 tctataaggg cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg
    23821 ctatcgatca atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga
    23881 ctaccacccg aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg
    23941 atcgaatgca ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg
    24001 atgatatggc gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc
    24061 actaaaattc gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat
    24121 ggtaacaaac catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac
    24181 atattatccg ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga
    24241 ccagcaagga attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg
    24301 cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg
    24361 cttgttagat agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg
    24421 tttttccctc ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac
    24481 catgattttt tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg
    24541 ataaatacaa cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta
    24601 cacttgttta tatagattat gttttttaat aaagggggat actaatatgc catggaacat
    24661 gaaggacttt cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga
    24721 catcgcaaat gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat
    24781 tgatcaagca aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa
    24841 agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct
    24901 tgattcagat gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc
    24961 aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt
    25021 agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga
    25081 aatcgattat tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat
    25141 gttcgaaaca atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac
    25201 gggaagagct gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac
    25261 ctctttttta ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg
    25321 cggaatcgtt tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca
    25381 gatttttctt tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt
    25441 ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta
    25501 gccgtgttct tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt
    25561 cgaccattct tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt
    25621 ttgattcaat aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa
    25681 taaaggtact cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat
    25741 atcttttggc atccattgat gaagatcgac atattgcatc ttattgactt catctttgat
    25801 tccttctgtt ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata
    25861 ttttggtgtt aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa
    25921 atattctttt gcaggagcaa gtgattcata taaatggatc cgtccatgga aatttttcat
    25981 ttttccgatt ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg
    26041 agcaatcaca cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa
    26101 cagttcatcc gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc
    26161 taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat
    26221 caaagggaat gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc
    26281 caatagttcg gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc
    26341 gattgagaaa gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct
    26401 ggataagaaa gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac
    26461 ggtttcgtcg attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc
    26521 tgcatcccaa tattgctgaa tgtctagttt tccatcttta tacgtataat aatggccttc
    26581 aggcaaacga tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt
    26641 catataaggt ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa
    26701 acttttgatt tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt
    26761 gatcccaaag tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa
    26821 agcaaacatt ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa
    26881 aaggacttct gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct
    26941 caaaggctgg taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt
    27001 gtaaataggc tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc
    27061 aacatgttta tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat
    27121 cattgtatta ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca
    27181 catttaactt tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc
    27241 atagaataaa tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag
    27301 atgaaagttt ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt
    27361 ttttgaggct accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa
    27421 aaaacagcaa aaaaagacac gacctaaaga tcgtgtctaa ttatgactgt atcagaaaat
    27481 atgataccaa acggatctat tcattaatta attgacatag tttatctgtt gctcttttat
    27541 gactggataa ctgaccaatt catgacgatc caagtcttct ttatttatat ttacagcagt
    27601 aatttgactg tcttcatacg tacgataata ataaatccct ttatcaacgt tacagcaaga
    27661 agaataaatc gtatattcgt attttccttc tccagtatcg catactcctt tttgttgctc
    27721 gactgagcct agtatatgaa aaaattgact gatactttca gattctgaat cacctgatgc
    27781 agcattcatt ttagtaaatg tggcttttac aaatcgagaa acagaagaga gatctccagg
    27841 taagcccaat cctcccatcc cacgactata aacattcaag ctgatctggt ttgaaaaatg
    27901 attttctggt gttccatttg atagcgagcg ataattgttc agattaaata attgataatc
    27961 aaatggcgga ttatttgtca acacaccgac aggattatca tataaatgaa gtccatcttt
    28021 catactttca attactatcg atttttcttt atcagctaat aaccagtgta aaggtgacaa
    28081 gggtagtttt tcactaaaat cgatattgac tagattgata gtagccaata attctttagc
    28141 ttcgtctatg gttgagcatt gccctagtat ccatggaata aactcaaatg gtgcaacgtt
    28201 atcttttcct tcttgtaatt ctttataatc agcattgcca ggaaaattca gtcctgccat
    28261 actcaaccct ttttcatttg tagcctcata atagagtgga tagttggata ccccagctgc
    28321 tattcctatc attgcataat gtttatctaa attttttacc tttctaaaat cgaaacgata
    28381 atttcttgga gtaatagtga ctacttcatt ataagacatc tcataatcaa aattccttcc
    28441 aaaataatgg tctttcgttg cataagtgat tgcggtacac atcgttttcc ctcctaaaac
    28501 aaatcgtttt tacactttta tcttacatct agtatttttt tttatcaaaa aaaaggccaa
    28561 agaagattga tttactagca tttataaaaa atagtactca gggggaatca aaaaatattg
    28621 taggttaaaa gtacaacaat aaggggctgt gacaaaagtc ttgtcacagc ccctt
//
LOCUS       DBNIBO010000017        27361 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.017, whole genome shotgun sequence.
ACCESSION   DBNIBO010000017 DBNIBO010000000
VERSION     DBNIBO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.017"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..778)
                     /locus_tag="AC1Y77_000678"
     CDS             complement(<1..778)
                     /locus_tag="AC1Y77_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIQ1574281.1"
                     /translation="MEEIDKLIAQYAYSKDDVYAEKYLKYPIFLDKHKKYKKMKQSTK
                     KVYMYLKKQNEYSLENSKVDSKGRIFLIFAIEKIAEKSCLTKKTVISALRELEEYKLI
                     TIKKNGFNKKTGKNNPNFYYLLKPEFSEGDFYKKKCIEENLHLPTNQHYSPSEKNTPS
                     DKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKESQNADFIDSLSVENKKLDEILIES
                     LIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWYKKFKYAI"
     gene            complement(982..1263)
                     /locus_tag="AC1Y77_000679"
     CDS             complement(982..1263)
                     /locus_tag="AC1Y77_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574282.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
     gene            complement(1314..1520)
                     /locus_tag="AC1Y77_000680"
     CDS             complement(1314..1520)
                     /locus_tag="AC1Y77_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1574283.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            1684..2415
                     /locus_tag="AC1Y77_000681"
     CDS             1684..2415
                     /locus_tag="AC1Y77_000681"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574284.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKELT
                     SFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            2469..3614
                     /locus_tag="AC1Y77_000682"
     CDS             2469..3614
                     /locus_tag="AC1Y77_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1574285.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(3699..4004)
                     /locus_tag="AC1Y77_000683"
     CDS             complement(3699..4004)
                     /locus_tag="AC1Y77_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIQ1574286.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(4029..4460)
                     /gene="ruvX"
                     /locus_tag="AC1Y77_000684"
     CDS             complement(4029..4460)
                     /gene="ruvX"
                     /locus_tag="AC1Y77_000684"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIQ1574287.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(4457..4723)
                     /locus_tag="AC1Y77_000685"
     CDS             complement(4457..4723)
                     /locus_tag="AC1Y77_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIQ1574288.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(4978..5703)
                     /locus_tag="AC1Y77_000686"
     CDS             complement(4978..5703)
                     /locus_tag="AC1Y77_000686"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIQ1574289.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(6064..6134)
                     /locus_tag="AC1Y77_000687"
     tRNA            complement(6064..6134)
                     /locus_tag="AC1Y77_000687"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6100..6102),aa:Gly,seq:ccc)
     gene            6251..7003
                     /locus_tag="AC1Y77_000688"
     CDS             6251..7003
                     /locus_tag="AC1Y77_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIQ1574290.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            7019..7498
                     /locus_tag="AC1Y77_000689"
     CDS             7019..7498
                     /locus_tag="AC1Y77_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIQ1574291.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(7749..9329)
                     /locus_tag="AC1Y77_000690"
     CDS             complement(7749..9329)
                     /locus_tag="AC1Y77_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HIQ1574292.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(9322..10065)
                     /locus_tag="AC1Y77_000691"
     CDS             complement(9322..10065)
                     /locus_tag="AC1Y77_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574293.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(10241..11017)
                     /locus_tag="AC1Y77_000692"
     CDS             complement(10241..11017)
                     /locus_tag="AC1Y77_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIQ1574294.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(11265..12101)
                     /locus_tag="AC1Y77_000693"
     CDS             complement(11265..12101)
                     /locus_tag="AC1Y77_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIQ1574295.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(12112..13041)
                     /locus_tag="AC1Y77_000694"
     CDS             complement(12112..13041)
                     /locus_tag="AC1Y77_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIQ1574296.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            13233..13586
                     /locus_tag="AC1Y77_000695"
     CDS             13233..13586
                     /locus_tag="AC1Y77_000695"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIQ1574297.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(13660..14316)
                     /locus_tag="AC1Y77_000696"
     CDS             complement(13660..14316)
                     /locus_tag="AC1Y77_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIQ1574298.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            14542..16476
                     /locus_tag="AC1Y77_000697"
     CDS             14542..16476
                     /locus_tag="AC1Y77_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1574299.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(16562..17785)
                     /locus_tag="AC1Y77_000698"
     CDS             complement(16562..17785)
                     /locus_tag="AC1Y77_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIQ1574300.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(17779..19650)
                     /locus_tag="AC1Y77_000699"
     CDS             complement(17779..19650)
                     /locus_tag="AC1Y77_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HIQ1574301.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(19671..20789)
                     /locus_tag="AC1Y77_000700"
     CDS             complement(19671..20789)
                     /locus_tag="AC1Y77_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIQ1574302.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(20872..21705)
                     /locus_tag="AC1Y77_000701"
     CDS             complement(20872..21705)
                     /locus_tag="AC1Y77_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574303.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(21698..22615)
                     /locus_tag="AC1Y77_000702"
     CDS             complement(21698..22615)
                     /locus_tag="AC1Y77_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574304.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(22842..23966)
                     /locus_tag="AC1Y77_000703"
     CDS             complement(22842..23966)
                     /locus_tag="AC1Y77_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574305.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            24149..25057
                     /locus_tag="AC1Y77_000704"
     CDS             24149..25057
                     /locus_tag="AC1Y77_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1574306.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            25198..26811
                     /locus_tag="AC1Y77_000705"
     CDS             25198..26811
                     /locus_tag="AC1Y77_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1574307.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8667 a         5259 c         4459 g         8976 t
ORIGIN      
        1 taatcgcata tttgaatttt ttataccagt cataaaattg actgtaatcg ttattaccca
       61 ctaatttcag acagtttaca atattatccc cccatacctg ataaagccat tctttttcaa
      121 ttaatgattc aattaatatt tcatctaatt ttttattttc aactgaaagt gaatcaataa
      181 agtcagcatt ttgagattct ttaaaatctt taaaatcttt agaagaatga ttaagttctt
      241 gtaaaaattt ttcacctgga agcgggaatg acttatcacc atcgaatgtt gatttgtcgg
      301 aaggtgtatt tttttcactt ggactgtaat gttgatttgt cggaaggtgt aaattttcct
      361 caatgcactt ttttttgtag aaatctcctt cgctgaattc tggtttcaat aaataataaa
      421 aatttggatt attcttccca gtttttttgt taaatccatt ttttttaatc gtaattaatt
      481 tatattcttc tagctctctc aaagcgctta ttaccgtttt ctttgttaaa caagattttt
      541 cagctatttt ttctatagca aaaatcaaaa aaattcttcc tttgctgtca acttttgaat
      601 tctctaaaga atattcattt tgttttttca aatacatata aacttttttt gtactttgtt
      661 tcatcttctt atatttttta tgtttatcta aaaaaatcgg atacttcaag tatttttcag
      721 catatacgtc atcttttgag tatgcatact gagcaattaa tttatctatt tcttccattt
      781 attcctcttc tttctaataa atgttaaaat gtagtcaaat aaagttttct tagaacttta
      841 ttgtcgagtg tttcctgtgg tcgccaaact gtcgggaaca ctccttttaa tttttttcaa
      901 agtattttta tattttctat acttcatcat tattcaaaca aacacttcct tttttctatc
      961 aaaagcacgc cacgcaactt tttatacttc atattctttg ataaaccttg ctaattcttc
     1021 ttttctaata aattgacaat ctccgattat gcttactttt aaacctttct ctctgataaa
     1081 tttttttaat gtacttctgc tacagcttaa atacttacaa gcttccccca atctaaaaac
     1141 ttctttgctt agagatgttt caattcgaac ttgatctact gcttcttttg caactcgata
     1201 gaaatatctt cgcaattctt gatctaaatc cgatggaaga ataacttgaa tctcagattt
     1261 cattttcttt gcccccacta atttgactac atgtattcaa attcaacttt tcgtcacttt
     1321 tcattaaaaa aaatttgatc aattgtgata ttttcaattc ctttttcttt cacaagatac
     1381 ttcaaaataa tcatttcttc tggagaatag tttgttttac cgctttcttt gtttcttacc
     1441 gcttgtactg aaatgccata tttttcagca agttcagatt gtgataatcc acaaaacttt
     1501 ctaaaatctt taggcttcat gactcctcac tcctccttct tttcaacttt tcgtcactaa
     1561 aagaataaca tatatttatt cataccgcaa cgtaaaattc aacttttatc tactttttca
     1621 tttcattata tgagaatgta gtgtaaactg tagaatagaa tagatgaagg tggtgaaacc
     1681 attttgtttg ataaaaacat taattctgaa attggaaaac gaatacaaga aatacgtgtt
     1741 tctttagggt atactcttga agaatttggc gaaaatctga cacctcccgc aagcaaaagc
     1801 aatgtttcta gatgggaaaa aggaaagaac atacccaaca aagcgcggct actaagtatt
     1861 gctaaacttg gtggtgtttc tactgaatat ctactgacag gtaattctca aaaaactaag
     1921 aaatatatca agtatttatt caatgagatt ttttttgaca acggtacctt ccataacaaa
     1981 ttcaaagatt tcggtaaaga cagagatagg cttggtctat atgacttatt tttagcagta
     2041 tcgaaaacat atagtctaga taaatcacct tctttaaaag ataactttgt agaaatgatt
     2101 atagatgatg tatataaaaa actcttgcaa actgataaat cagatgacca actcatcctg
     2161 ggtttcatat atatttgtct tgatgaaaaa ctttcaaaca gacagaaaag tcttaaaagt
     2221 atcaattatg aggtattcaa ttatttaggt gttgcatttg gtttaattga tagcgaaatc
     2281 aaatctacaa acgcaggtaa cgatgaagat ttattaaatt atttaaaaga attaacatct
     2341 tttattgatg aaataaaaac aactacattc aacaaaaaaa aatattttca aaatcaatat
     2401 gatatagaag aataaagatt acattttcct atcaaaagca cgccacgcaa ctgataggag
     2461 gaaaaacaat ggcaacaatt aaacaatatg aaaagaaaga cggaaccaaa gcttggatgt
     2521 ttcaaacata ccttggaatc agcccgctaa ctggtaaaga agtccgtact acacgtagaa
     2581 attttaaaac aaaaaaagaa gctcaaatag agcttaatag actacttgta gactttgaag
     2641 agaatggact taaaagggaa acgaacgaaa ctttccaaga agtgtatgat ctctggtatg
     2701 aaagctataa aactactgta aaagaagtca ctcaattaaa aattgagatc aagtttaata
     2761 aatggattct tccaaaatat gggcagttta gaattaaaga aattactaca aaacatgttc
     2821 agaagatatt gaatcagtgg gctaaaacaa cagatcaata taaagtccta cattctaccg
     2881 taagccgtat tttcaaatac gcaatgactc tagggctgat cactaaaaat ccctgtgagc
     2941 tcgtaatcat gcccaacaga aatgttgaaa aaaagaagga tactgtcaaa atttattcac
     3001 gagatcaatt agaaacctta tttcaatatt tggaatccaa aagaagtacg tatagggcag
     3061 agtacgataa aactttactg cgctttctat tttttagtgg ctgtcgaatc agtgaggctt
     3121 tagccttaga ctggtcagat attgactttg attcaaacac cgtcactata aacaaaacct
     3181 taagccaaac aaaatatggg tacaaaattt caagtccaaa aacgagaaat agctatggtt
     3241 ccctatcttt agacaataaa accattaatt ggctaaagaa atggcaaatc aatcaaaaaa
     3301 gatatatgtt aaccctcggg attactgatc caacaatgat tttttgtgga atctacaaag
     3361 aaatcattac taaacacgct atctattctc gtatgatcac aatctgcaag aattctggta
     3421 ttccatttct aggtaaccat gtaaccagac acactcatgc tagtatgctg ttagagtcgg
     3481 gagtctccat gaaggaagtt caagaacgcc ttagacactc atcaatcaaa ttaacaatgg
     3541 atacctatgc tcatctaact aaagaaacca aagaaaaaac cgttgaaaag cttgcagagc
     3601 acctaaattt ctagcggaac gtattcaatc ccgtattcaa ttcaaaacaa aaaaaagctg
     3661 aacccttatc taccaagggt tcagctatca tttacagctt attcttcttc ttccgccatg
     3721 aatgtattga atacttcttc aatcatgtcc cattctgcgt ctgtttcgat ttgttctaac
     3781 tcgccttctg ttccatcttc gttttcgatg tatgcataag cttgtaattc tacatcttca
     3841 tcttctgaaa caccagcagg atataaaaga acatagttac gtccaaattc ttcttgtccg
     3901 tcaactgtta gtaggatttc atataatgtt tcatttcctt ggtcatccac caatgtgatg
     3961 tgttcgtgtc cttcatggtc gtgggaatgg ttatgttctg tcataagatt acctcttttc
     4021 ttttattatt aaaataattg cttacctgag ccgtctaaat aattttgtaa gatcatcaca
     4081 gctgctaatt tatcgatgac tttttttcgt ttggctcttg aagtatttgc ctgctctact
     4141 agcatacgtt ctgcctgaac agtggttaat cgttcatctt gataatcaac tggcaaggag
     4201 aatagttctt tgatcttttc tccgtatgac atggaagctt ctgcccttgg tccgatggaa
     4261 ttgttcatat ttttaggtaa tccaactaca aaccggtcta cttcatattg tgctactaat
     4321 tccttcaacc gatcaaagcc aaactcttct tctgcttcat tgatccgaat gatctctact
     4381 ccttgagctg tccagccaaa ggggtcactg atagcaacac ctactgtctt agaaccgaca
     4441 tccaagccca ttactctcat aaattcactc catgatttga gaggtagtcc ttgacaatta
     4501 cttccatgat ctcatcgcgt tcgtgacggc gaatcaaatt tcgagcatca cgataacgag
     4561 gaatgtaggc cgggtctcca gaaagcaagt agccgacaat ctgattgatt ggattgtagc
     4621 ctttttcttc caaagcccga taaacaatcg ctaatgtttc gctcacttct tttttgcgac
     4681 tatcgtcaaa accaaaacgt acggtttcat ctgtaaaacc catgattcta cacctctttc
     4741 ccgcaaggta attccagctt accttctaca accttatttt actcgaagaa agggaaaact
     4801 acaaacaaat attccaaact tccgaataaa ttaagaaaca ctatttgtat gtttttttcc
     4861 ttttttctgt agctttatca aaagtactct tttcataaag tatacgctat atttctttga
     4921 atgtatgttt ttacacaatt ttttcataat attcataaaa aattcgaata gctgctacta
     4981 attttttgtt tttgttgctt gtcctgtctc ataattgatc gtgtagccat cttgaacatt
     5041 gtagataaag acattgaatt caattttttt gtcttcaatg ctttctgctt ccaattgcac
     5101 tcccctggct accagttcat tcccctcaaa atgaggagtg acacggtagc gtacatgatg
     5161 atttgtttct ttgatatatg ccgcaatatc attttcgtga gcaagcatgt atggagcatt
     5221 caacgaacgt gtccctgtca tcaagttttt aatgttgtta ttttgaccag tcagctggaa
     5281 accaattaaa tggctgcgat tatataacca ttgcccgtca cttaatttct tttgtttcca
     5341 acctgtcggg tcaatataca aaggctctct cttttcagta gggaacaaat cttttccaag
     5401 catagcattg gcttgtccta ctctgttcaa tttatcaata tttgaatagc tttcccatgt
     5461 tcctttggaa aggctaagat cctcttttgt aaattccggc tgattcccat tgatttctat
     5521 ttcttgatgt tttccatcat attctggcac atccgctact tgagaagctt gtggtgtgga
     5581 atcaaaaata gttgtaatct gctgaaagat atctgaatct ttttgtgtgg tggaaacacc
     5641 gaatgcccct gcaatcacag cagcgacgat ccccacgatc gccttggttg tttttttgct
     5701 catttcttta ctcctttact aacagctccc taaatcttaa catatgttcc cctataaata
     5761 aatactagaa agaaatagaa acagcaaaaa ataaaacctg ttttaagatg gcttaaaaat
     5821 acaaaaaaga gacggattcc tcgtctctaa tcagtcacca ctctatctgt aaggtattta
     5881 ttattattca aaaattttct tcatactcaa gacaatacac tgatttcaca accgcttggt
     5941 aaacggtgcg caaaagcttg catctgcggt aataagtcca tccaagttaa aaacataaga
     6001 agttgttttt gcttggctgt tcttattact tgttgcagag cagctttttc acaacctatt
     6061 ttaagcgggt gacgggaatc gaacccgcga catcagcttg ggaagctggg attttaccac
     6121 taaactacac ccgcaataca aaaacagtat acgcctcttt tattccttcc tcaagttttt
     6181 ttattcttta cgcttctggt atgttttgta gtaaaataaa actaatcaat caattattgg
     6241 agaagatgct atgacgggaa aagaattaga tctgttgctg gacacttgtt tactcgctgg
     6301 gaaaatcatg atggaaagca atgcggagat gtatcgggta gaagatacga tgagtcgaat
     6361 tgctttagct tctggaaatt tccgtttggt cagctatgtc actcaaaccg gtctctttat
     6421 tggtcttgat cgtacctcga ccatccgaat ggaacaaatt accaaccgtt cgattaattt
     6481 agaaaaagta gtaaaagtaa atgatctttc acgtaaatat gttgcttcag agctaacatt
     6541 agaagaactt tactgcgaac tgcaagcaat cgaccgagac cgttctttct ttcctgtctg
     6601 gttgcaaatc gttagcgcgg cagtgatcag tggaacaatt atggtgttat ttggcggaaa
     6661 gatgaccgat ttacctgtga ctttagtcat tggaggattg ggatacgcag cttatctata
     6721 tagtttgaaa ttttttagaa tcaaattttt atcggaattt ttatcttctt tattgattgg
     6781 catagccgct atctacagcg tacgttttgg cttaggaagc aatcaggatc taatcattat
     6841 cggatgtgtg atgcctttag ttcctggagt tcaaattacg aacgctatcc gtgatttgct
     6901 agctggacat tatgtctcag gagtttctcg cggaacagaa gcgatgatga ctgctacgat
     6961 gatcggtttt gcgattgctt ttattttcca gcttttttat taggaaagga acacaagtat
     7021 ggggacgata tttattcagt tttcttttag ttttttagcg actgcggcat ttgccgttat
     7081 tacaaatgta cctaggcgtt cattgatttg ttgtggtctt tctggtgctt ttggctggat
     7141 gatctactgg atctgtgttc aaataggcgg atctgctgct tttggttcat tgcttggttc
     7201 tttaggagta gcattcatca gcgatctttt ctctaaacgt ttgaaaatgc ccgtaacgat
     7261 tttcaatatt cccgggatgg ttcctctagt ccctggtggt ttagcttatc aagcggttcg
     7321 aaatctcgtt actgaatcct atcgagaagc ggctcattat gcggtccaag caattatgat
     7381 tgctggagca atcgctttag gtcttgtatt atcagaagtc tttaaccata acatccgtaa
     7441 tttcaaagta aagcgtgacg caatcatgat gaaacgaaaa aaacggcttt ttaaataaac
     7501 gaagtgtaac aaagaaaagg ttgtgaaaga gacgttcagc cttgagcgat gacaaaatat
     7561 ctcacaaaca ttgcgacaag taaccgcagg agcaataaga agtaattatg agtattaaaa
     7621 aaagttttgt aaaaatagct tctcacattt ttacataaaa ttgacttaat actgagaaac
     7681 tgacttttga acaccctcta tctgtaaaaa aggggctgcg acatgatttt tgtcacggcc
     7741 cctcttcttt attcaaattt tttccaaaac gttcgttgat aataaacgat ccatagagcg
     7801 gaaagtatta gaactaaacc gatcacagga atattcacta accaaaatgg ataaaacaaa
     7861 gccaacattc cgtataagac gataagaata atactgatga tcaatgaacc catcatagtt
     7921 gcgactaaac ccaaacttcc tgaacctctg ttaaataatt ggttaatatt ggtccagttc
     7981 agcagcagca accgataatc tctagcgaaa taacgtagtg acaataagta gcagccgaat
     8041 aaagaaccaa atagcaagct agggataaaa aacaaaggca gctgaaaaga cagtccgctt
     8101 aataatgcga tcccaccagt taaaaaactc tgcaatatcc atccgaatct gaacttttct
     8161 tttaaatatt ggttcataga gattggcaaa gaacggataa acagaaaatt ttcttgatcc
     8221 aaagaaataa gattgctgat aaaagaagtc tgattaactg ttaagaaagc caatgcgatc
     8281 cctaccagga aaacaacacc gattaactta ttatctaaat cagttaattt tatttcgcct
     8341 cctagggcaa aagcaagaat aaaaatgatt ggagttaaca aggaagaaga aagaacttgc
     8401 ataatcaaat taggttcttt tactaattga gcattgtaac taaataaaag ctgatgcaca
     8461 ttttggttct ttttatgggt tctttttatt ttgaactcac tgtttgaatc agtcagttgc
     8521 tctgtaagct ttggtactat cagctgtttt acagcaagga aaaatcccag agccgtgata
     8581 gctagtattc cccaacccaa taaaccttgt tgagaaagtg gctgactggc agcatcatag
     8641 aatggcaata atatcgaaat cggatgccga tctgttaact cattttcaat agtccccgtt
     8701 tgatgattca tccacatgat tccagcaaat gcgatcagca tcgagctgcc aagcaagata
     8761 ctagaaactg tttttctatg ttttttaaac actgctgttc gagccaaacc aaaaacaatc
     8821 aaactagcca gacaaaaaag aataatcaaa aatagagaaa acaaaaggat tgagataatg
     8881 atagtaagca aaatccctag atcagctcgc catccggtta aaataaacat gactagaagc
     8941 ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa tctttgctga aaaaacctgt
     9001 tcttgtccaa aaggcagagg caaaaatact tgcaggtcat ttccttcaaa aaagatatta
     9061 taaatgactg atatactttg agttaaacct aacataccaa aaagtgccac gtaataagta
     9121 aagaaacctg gcaatttaac aaagttcata gcaaacatag caaatccgta aatcagtaaa
     9181 aaaatcagcc ctgaaaaaag attttgagac aaaagataga tagttaatcg ctttcccttt
     9241 tttcctttct ctcttgcttt attcgtgagc tgtggattaa catataaaag attaactcga
     9301 attaattcta ataattgtcg cttatacata cggtccatct cctgtcgctt gccttccagc
     9361 cattttcaaa taaatctttt ctagcgtttc atcaggtgag ccattcaata attcactaac
     9421 tgccccctga taaatcaatt cacctttctt caaaatagcc agtttatcgc ataactgttg
     9481 tgcggtatcc aatacatgtg tagaaaaaat gactgttttt ccttttgcgg catgagcctt
     9541 catcatttct ttcaaatcaa atgctgcttg aggatcaagt ccttgcaaag gttcatccaa
     9601 tacccaaata tctggatctg gcagcaacgt gccgatcaaa atcgtttttt gacgcattcc
     9661 atgtgaaaaa cttgcgattg tttcattttg atgactatac atatcaaaaa gcgctgtcaa
     9721 ttctgctaaa cgcttttctt ttttttgtgt gtctaattca taggcagctg aaatcaagtc
     9781 ccaatattct ccagccgtca attgtaaaaa aatatctggg gaatcaggca cataaccgat
     9841 tttttgtttg atactcaatc gattttccgt caatttcatt ccatctacta aaatcgttcc
     9901 cgatgatggt tcaataatac taactaaact tttaatcgta gttgattttc cggcaccatt
     9961 atgtcctaaa aatccaaaaa tttccccttg tcttatcgag agagacaagt cttttaatgc
    10021 ttgttttttc ccgtaacttt tacttacatt gatgaattca atcatttctg tactcctttt
    10081 gtttgttatt actttttatt gtacccttcg ttattggtgg agtaaatgtc aaaaagagtg
    10141 cataacaaaa aaggatagaa acgactaggc tgctacttgg gttgttctct tctacaggaa
    10201 aacactcttc taacaggtaa gatatgagtc tttgttttct ttaaaatgat tcattttcta
    10261 tcttttcttg cataaaattc tttagtttat caatacttct ctttcccatc tctagttgtt
    10321 ctccatttgc aaatgttact atttcgttta ctctgctaat agatgtaatg ttttcagtat
    10381 taatgatata agcttttaaa tctttacata aatactcata ttcgagtgat tttttatatt
    10441 ttttaattgt gccgtttacg agaatctctt catctttcaa tactaggcct attttatttt
    10501 tataacctgg catgctcatt agataaagga tttcatcgta atggatatag tgatcgctat
    10561 taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga tagttttctt
    10621 tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg aattctttta
    10681 tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta ttcggatgag
    10741 acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg cctagttcga
    10801 taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag gaatagattt
    10861 cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg atctcaaatt
    10921 tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt ttttcaaaaa
    10981 ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc actcgatatt
    11041 ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata aattgtaatt
    11101 ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca ggatgctgct
    11161 tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac aaccgacgtc
    11221 cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa agaacgatac
    11281 acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac agatgggatt
    11341 tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt ataccattca
    11401 tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac tcctgaatct
    11461 gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct atcgttcggt
    11521 gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct agggtaatta
    11581 taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt ttgcttatcc
    11641 ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt gaaataaata
    11701 ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga ttctttactt
    11761 actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac tgttggaaac
    11821 aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg ttgataaggt
    11881 tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt tccgtttttg
    11941 acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga aagaatagct
    12001 aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg caagtacatc
    12061 atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct cctaaataaa
    12121 taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa taaagcgtcc
    12181 tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc caaagaccaa
    12241 aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta taaacgcatc
    12301 tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa tggctaaaat
    12361 ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca tcgcagctgc
    12421 gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga ttgaatgtgt
    12481 caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct cactttgttc
    12541 ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt tttctttcaa
    12601 tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa caaaaaagct
    12661 gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa aagtggaaaa
    12721 aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg tgtgaactgg
    12781 tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac atgaaggaaa
    12841 gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag gaagccatct
    12901 cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac agcctaataa
    12961 tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct gcaaaaccga
    13021 atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat tcgctttgca
    13081 aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac aactcttatt
    13141 ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa ggtctcatac
    13201 atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac ttgactgaag
    13261 ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg cctaattgca
    13321 gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat gatattccgg
    13381 cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa gtgctgactg
    13441 cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca cggttcatcg
    13501 actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat tccctttctt
    13561 atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg attgaacaag
    13621 tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg tattttcgga
    13681 atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat tcgtcaaatc
    13741 aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa agttcaagat
    13801 tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt cagcgggtat
    13861 cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat cttccattgg
    13921 ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat caaaagcaca
    13981 actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac ttcccaagtt
    14041 ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct ttgcaaaaaa
    14101 gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc caaaataaga
    14161 aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt cagttgaaga
    14221 cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca aaggcagtct
    14281 tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac gtattgtcta
    14341 attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa ttccattgct
    14401 cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa atctcgcagt
    14461 ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga ggagaattca
    14521 caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc tcgccatttt
    14581 ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc tcgtgtcgct
    14641 ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc tggaattgaa
    14701 gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata cctcgctcag
    14761 gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc ttttgaacaa
    14821 gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga aggtacgcca
    14881 gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca cgatttttca
    14941 gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg gtttaaattc
    15001 gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg cacacgttta
    15061 gctttagctc gcatgctttt gcaacaccct gacattttga ttctagatga accaacgaac
    15121 catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta tactggtgca
    15181 ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga agtctatgaa
    15241 ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct agatttgaaa
    15301 gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga aatccataaa
    15361 ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg agcgcaaagc
    15421 cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga tgaaaaacaa
    15481 gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca aatcgaagat
    15541 gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga tatccgtcgt
    15601 caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt gctgaaatca
    15661 atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa tgtcaccatc
    15721 ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct agcagaattg
    15781 tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg tgctttctta
    15841 ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga aaaagcacgg
    15901 gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga tgaaccaaca
    15961 aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga ttatgacggc
    16021 acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac aaaagtcgtc
    16081 gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta tttagagaaa
    16141 aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga atctgctcct
    16201 gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct tatccgtagc
    16261 cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga atggatcgat
    16321 cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa actcaatgag
    16381 ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac ggaatgggaa
    16441 gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa cagaaaccat
    16501 tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat atttaccttt
    16561 ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa taatagtaat
    16621 caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa acagactgtg
    16681 ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt cgcataattt
    16741 tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct gattctatta
    16801 attctaataa tccacaaacc atgatcacca tactagatgt atctttttct tctctgctag
    16861 aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga ttcataaaat
    16921 aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa cgataactta
    16981 atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta tcactataac
    17041 cttgatgcgt acttccatat cttggttttc catctttgac atccatataa aatgtatgat
    17101 aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga gcctgagcag
    17161 cttttttata tttttgattt ccagtttctt cactcgccca atacaataat ggaagattca
    17221 tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct ccccaagctt
    17281 gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca tcaattgaag
    17341 cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat aaagaataca
    17401 aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg ataaaatcta
    17461 tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca aataacaacc
    17521 aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat ggataacgtc
    17581 cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt ttgattaacc
    17641 tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc attaattctt
    17701 tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc actttatcaa
    17761 gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt tattttggat
    17821 agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata cttctaaact
    17881 ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc cttttccaaa
    17941 tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg tattgggcaa
    18001 acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga taatacctga
    18061 aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta ttccttcttt
    18121 ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg tatctaattc
    18181 tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc tgatttttgc
    18241 ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa tgtctcgtac
    18301 attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa aactggtcgt
    18361 gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt ctgctttcaa
    18421 ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt ttaaatattt
    18481 ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa aaaatgtatg
    18541 tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc cttcacgaat
    18601 aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg ccaacaataa
    18661 aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag tcaatgtcaa
    18721 ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt ctttttgcat
    18781 ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa aaggcaaaag
    18841 atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa tcatgcttcg
    18901 accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc tagaaaaatc
    18961 aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta cgctataaat
    19021 caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc ttgaactgtc
    19081 tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt ctttcagtgc
    19141 tgattcttta actggataac caaatttttg gataattgtt ccgacaatta tcttaaaaaa
    19201 ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat gaataatctg
    19261 gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat ataacgctaa
    19321 taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc cccaataatc
    19381 gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca accatcgttc
    19441 atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc cttccattcc
    19501 tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg cgccacttgc
    19561 tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt aatcctgtct
    19621 ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta ctaccaaata
    19681 gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc agaatagatc
    19741 agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc aatgatccca
    19801 tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat attgatagca
    19861 gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc cagaagaccg
    19921 catgcagcgc atactccagc actggtatca atattcgaat cttttacagc aaaatcaatg
    19981 agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat cttttctgct
    20041 atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc atataaagcc
    20101 catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc aagtaaccga
    20161 atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc atctttcctc
    20221 actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa cctcggatca
    20281 ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc aataatcatc
    20341 caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt atagcgtcca
    20401 gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg agactgttga
    20461 taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac gtctgaaaaa
    20521 tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt gaacttttct
    20581 cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt ggtccaccag
    20641 tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat atatggaatt
    20701 ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt caaaattttt
    20761 cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta atttttatga
    20821 atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt tttacttatt
    20881 tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa tcaagaccat
    20941 tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg gctcacctgt
    21001 actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt tatctgaaag
    21061 catgactaat ggaaatagat attgattcca acttcccata aagataagta aaccactagt
    21121 aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc actcattggc
    21181 accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat attgtcgcat
    21241 cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat aagtattcga
    21301 taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa aaggcaccat
    21361 cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat actttgctaa
    21421 tgcataaccc gccattgtag aactggctgt agctaaaact gtactgatta cagcaataac
    21481 aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt gccagttttt
    21541 taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt gattactttg
    21601 cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac caaacacaat
    21661 aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta tcacctccga
    21721 atttaaattg aatagccgat aatataacag atacaaacac catcgtccag gcaatagcag
    21781 aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact cctacgacca
    21841 aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata ttgaaattac
    21901 ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt agcatgggga
    21961 tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca ctttcgtata
    22021 tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat cctacactaa
    22081 gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt aaccactgta
    22141 caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga ttgaaaaaag
    22201 tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa tagacagttc
    22261 taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag taaatagcta
    22321 atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg taagatttaa
    22381 taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat tcaaatgagt
    22441 agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa atcgtcacaa
    22501 taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga gacgtatctg
    22561 ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgt atcatcgtta
    22621 agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg agcaaaattt
    22681 tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt atatgcagcg
    22741 gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt tgatgtattt
    22801 tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc aaactttttc
    22861 tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag ctccagtgcg
    22921 ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc cacttgtgga
    22981 tttttggcat ctttagggac ataaaaactt gatcctcctg atactgcacc ttttcctgga
    23041 ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc atcaggaaaa
    23101 ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga ttcttgaaat
    23161 gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat catttctttg
    23221 attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc ttcttcatca
    23281 agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc cccagattct
    23341 ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac ctggatatat
    23401 tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt ttttggttga
    23461 taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc attactcgtt
    23521 tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt gtcataaaat
    23581 ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc tgctgctgaa
    23641 gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt tggggtgatc
    23701 atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat atttgctcca
    23761 gttttgcggt tatagatttt tgcagcattt tcaacatagt ttgtcagttt gccgtttgcg
    23821 acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt tgctgttact
    23881 tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt tagcaaagct
    23941 atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag ttcttatata
    24001 aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat ataaaaaaat
    24061 attttacttg tcgttttggc ttttcatttt tgttataata gtcattaatt ataaatagaa
    24121 agctttttat ataaaaagag aaaggaaagt ggttcttttg ttaacaaaca accaatcctc
    24181 caatcttgtc aaaaaaacac taacctattc catagatacc catcttcatt tatctgctgc
    24241 tccctatttt cgactactaa taaatgaaag cgctaattta tatgctgaat ttgcacataa
    24301 atctatagaa ctagataatc cttccctttt attcattgat aaacagcaag aagtccaaat
    24361 cttttctact caaaaggaaa cacaaacttt tatcattatc cagttttatt ttgatgagca
    24421 attattagca catattacgg gacaatcttt ttctatagat caagaaataa catctatcat
    24481 tacttttctg aaaaatgaac agttcaaaat tgattatcta aaacatagta agccagctat
    24541 ttcctatgat caggaaatca attctttttt catactatgc caacagatga gcattcattt
    24601 gctttcctta ttagttactt attgtctgca aaaaattcaa ttgtcttcct taccaaggca
    24661 ttatcaacag gaacttgctt tattgacaat accttcaaat gagaaagtca ttcctaaaaa
    24721 aaagcatatt ctttattcag atgaattagt tgatccaatc atacgatatt tagaagaaca
    24781 tctttcagaa aattttttgc ttgaacaatt agcccaaatt ttttttatca gtccagccag
    24841 cttaaaaaaa cattttagaa aagtgaatgg aaaaagtgtt attagctatt ttaaagaact
    24901 aaaaatggaa aaagcaaaac gtctcgtgct agaaaatgaa ttaacctata cagaaattgc
    24961 agatatatta ggatttcatt cgatccatca tttttcttca gcttttaaaa aatatacagg
    25021 gttatcccct tcaaagtatt atctatcctt aaaatagaaa aaaattttta ggttttatcc
    25081 acaaattatt tctctatatt ttctgtctat tcaaaataaa caataatttt ccggcacaat
    25141 tatttgctgt tttttaccat aacatagtac gatcaacgta gaaaaggaga tgtttttatg
    25201 aaagttgttg tcattggcgg tgtagctggc ggtccttcat ttgccactcg tttccgtcga
    25261 ttgaatgaag cgcacgaaat cattatctat gaacgcggag aaaatatttc ttatgcaagt
    25321 tgtgctttgc cttattattt aggaggtgtg atcactgacc gtgactcgct gatcgaacgt
    25381 acaccagaaa tattgaaaac aaaaaacaac atcgatgtct ttactaaaca cgaagtaaca
    25441 gcaatcgatc cttctactaa gcgattaaca gttaaagacc tatccacaaa tgaagaaata
    25501 aaaacagatt acgataagtt gatcatctct tctggtgcta gaccagatta tccggatatt
    25561 cccggagttt ttgaagcaga aaacggcttt gtcctccgta gtgtgacgga tgcggatcga
    25621 atcaaatcgt tccttgaaga aaaaaatcca caacatgtcg tcattcttgg tgcaggtgtt
    25681 atgggtctgg aattagctga gaatttcaag catcgcggct tagacgtgac tttaatcgat
    25741 caattgccac aagtcgcttt cccttatgat ccagaaattg ctaatttagt ttatgacaaa
    25801 ttgctgaaag aaggattagc cgttcattta gaaacaagag ttactgagat ccgtgataaa
    25861 ggtcgagaaa tagtattatc agatggctcc gtcctttctg ctgatatgct aatttttgct
    25921 gttggtgttt ctccgaagaa tgaagtggtg aaagcaaccg gcatacaatt atcagataca
    25981 ggtcagatca ttgttgatga ccagttacaa accaatctgc cggacatcta tgcgattggc
    26041 gatattatcg aaacgactag tgtagtgact ggtcagccga tccaaagtat gctttccagt
    26101 gcggccaatc gtcaaggaca tatgctggca gatattttaa atggtacgcc tatgcgctat
    26161 cgcggatata ttggtgcagg tgtcgcaaaa atctttgatc atacagcaag ttatgcggga
    26221 atgacagaac atgaattaaa agcatcaggc ataacaaatt ataaaactgt ttttatcact
    26281 ccctttgacc atgcctattt ctatccagaa gctacaagat taaatctaaa gctgattttt
    26341 gatgcagata gcggtcgtat tttaggtgga caagcatttg gagaaaaagg tgtcgataaa
    26401 cggatgggag aactttctgt agcaatcacc ggaaacttga cagtctttga tttgcctgat
    26461 ttggagttgc cttactctcc accgtattct actacccgtg acccgttgaa tatagctggt
    26521 tatgtcgcaa tcaatcaaat gacgaatatc gtagaaacga tcaaagcaag tgatataccc
    26581 gaaaacgatt tgaaagaagc gttcttttta gatatacgtg aacctaataa agcaccttct
    26641 ggcagtatct cggcaacaaa aaatattcca atgaatgagt tacgagatcg aatcaatgaa
    26701 atcccaaaag ataaaaaagt ttacattact ttcagaagag gattgaatac ttatacttct
    26761 gcccgaatct tggcaggttt gggtatcaaa gcgattttga ttgaagaata aaaaaacgac
    26821 cgcggtgggt agccgcggtc taggagttat ttttacttga ggagtaaaaa tgaaaaagta
    26881 tatagggttg ttggttggta tgtatataga atagaagata aatgtgaaga aattgtgcat
    26941 tatttcttca catttattag gttatagttt aagaaagttt aaacattgcg caatttaatt
    27001 attttagctg tttcttttca taaaaaaaca tgttgtgact ctgagacaaa tcatagtcca
    27061 tagtgatgtc tctagagtaa ctacgcctaa tattacgagg catcatatta atctatcagc
    27121 actagtacct aaactagcat ccctttcgaa tattttttct tatataataa agtttgcttt
    27181 cactcctcta atctcaccaa atcgactggc ttgcgttgtg tgatactagg aactgattgg
    27241 tttagaacga cttttctatt gtaatcatat ttttcgccat cctcgttgtc ttctaatcct
    27301 tcaatgaatg atccctaatg tgtgtattaa ataatttaat ttttttgttc ctatttattt
    27361 a
//
LOCUS       DBNIBO010000018        27109 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.018, whole genome shotgun sequence.
ACCESSION   DBNIBO010000018 DBNIBO010000000
VERSION     DBNIBO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.018"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(50..595)
                     /locus_tag="AC1Y77_000706"
     CDS             complement(50..595)
                     /locus_tag="AC1Y77_000706"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIQ1574308.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(607..1299)
                     /locus_tag="AC1Y77_000707"
     CDS             complement(607..1299)
                     /locus_tag="AC1Y77_000707"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIQ1574309.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1318..1602)
                     /gene="macP"
                     /locus_tag="AC1Y77_000708"
     CDS             complement(1318..1602)
                     /gene="macP"
                     /locus_tag="AC1Y77_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HIQ1574310.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1615..2178)
                     /locus_tag="AC1Y77_000709"
     CDS             complement(1615..2178)
                     /locus_tag="AC1Y77_000709"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIQ1574311.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2406..3068
                     /locus_tag="AC1Y77_000710"
     CDS             2406..3068
                     /locus_tag="AC1Y77_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIQ1574312.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3192..4388
                     /locus_tag="AC1Y77_000711"
     CDS             3192..4388
                     /locus_tag="AC1Y77_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIQ1574313.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4489..5865)
                     /locus_tag="AC1Y77_000712"
     CDS             complement(4489..5865)
                     /locus_tag="AC1Y77_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1574314.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5902..6768)
                     /locus_tag="AC1Y77_000713"
     CDS             complement(5902..6768)
                     /locus_tag="AC1Y77_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HIQ1574315.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6857..7882)
                     /gene="sapS"
                     /locus_tag="AC1Y77_000714"
     CDS             complement(6857..7882)
                     /gene="sapS"
                     /locus_tag="AC1Y77_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SapS"
                     /protein_id="HIQ1574316.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7900..8574)
                     /gene="sapR"
                     /locus_tag="AC1Y77_000715"
     CDS             complement(7900..8574)
                     /gene="sapR"
                     /locus_tag="AC1Y77_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SapR"
                     /protein_id="HIQ1574317.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8758..9651)
                     /locus_tag="AC1Y77_000716"
     CDS             complement(8758..9651)
                     /locus_tag="AC1Y77_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1574318.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9725..11422)
                     /locus_tag="AC1Y77_000717"
     CDS             complement(9725..11422)
                     /locus_tag="AC1Y77_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIQ1574319.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11415..12728)
                     /locus_tag="AC1Y77_000718"
     CDS             complement(11415..12728)
                     /locus_tag="AC1Y77_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIQ1574320.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12728..16504)
                     /locus_tag="AC1Y77_000719"
     CDS             complement(12728..16504)
                     /locus_tag="AC1Y77_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIQ1574321.1"
                     /translation="MKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNG
                     GANWRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVL
                     PSSTASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTK
                     NAALIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLT
                     GSENTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPI
                     QSSYRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRP
                     EDEVNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNK
                     YQENPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIV
                     TALEDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIW
                     DEERHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFC
                     TVNNMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFAL
                     YDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLV
                     DETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQE
                     PNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGY
                     PVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTN
                     YAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDR
                     LWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEE
                     SKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFAL
                     NEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLE
                     GAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPG
                     YFILEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNR
                     IRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16539..16997)
                     /locus_tag="AC1Y77_000720"
     CDS             complement(16539..16997)
                     /locus_tag="AC1Y77_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1574322.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17295..17813)
                     /locus_tag="AC1Y77_000721"
     CDS             complement(17295..17813)
                     /locus_tag="AC1Y77_000721"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIQ1574323.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17815..18432)
                     /locus_tag="AC1Y77_000722"
     CDS             complement(17815..18432)
                     /locus_tag="AC1Y77_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIQ1574324.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18429..19283)
                     /locus_tag="AC1Y77_000723"
     CDS             complement(18429..19283)
                     /locus_tag="AC1Y77_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574325.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19280..20191)
                     /locus_tag="AC1Y77_000724"
     CDS             complement(19280..20191)
                     /locus_tag="AC1Y77_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574326.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20232..21566)
                     /locus_tag="AC1Y77_000725"
     CDS             complement(20232..21566)
                     /locus_tag="AC1Y77_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574327.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21774..22604
                     /locus_tag="AC1Y77_000726"
     CDS             21774..22604
                     /locus_tag="AC1Y77_000726"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HIQ1574328.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22848..23097)
                     /locus_tag="AC1Y77_000727"
                     /pseudo
     CDS             complement(22848..23097)
                     /locus_tag="AC1Y77_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23288..23482)
                     /locus_tag="AC1Y77_000728"
     CDS             complement(23288..23482)
                     /locus_tag="AC1Y77_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574329.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(23987..24172)
                     /locus_tag="AC1Y77_000729"
     CDS             complement(23987..24172)
                     /locus_tag="AC1Y77_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574330.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25022..25363)
                     /locus_tag="AC1Y77_000730"
     CDS             complement(25022..25363)
                     /locus_tag="AC1Y77_000730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIQ1574331.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25363..25593)
                     /gene="mazE"
                     /locus_tag="AC1Y77_000731"
                     /gene_synonym="pemI"
     CDS             complement(25363..25593)
                     /gene="mazE"
                     /locus_tag="AC1Y77_000731"
                     /gene_synonym="pemI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemI/MazE family
                     antitoxin"
                     /protein_id="HIQ1574332.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            25880..26218
                     /locus_tag="AC1Y77_000732"
     CDS             25880..26218
                     /locus_tag="AC1Y77_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574333.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26423..26985)
                     /locus_tag="AC1Y77_000733"
                     /pseudo
     CDS             complement(26423..26985)
                     /locus_tag="AC1Y77_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         8074 a         5782 c         4611 g         8642 t
ORIGIN      
        1 cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt
       61 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
      121 ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      181 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      241 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      301 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      361 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      421 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      481 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      541 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      601 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      661 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      721 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      781 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      841 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
      901 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
      961 tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
     1021 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1081 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1141 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1201 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1261 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1321 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1381 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1441 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1501 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1561 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1621 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1681 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1741 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1801 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1861 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     1921 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     1981 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc
     2041 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2101 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2161 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2221 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2281 tctaaatata gaaaatcctt ctttagtccg atactcttta tagtagtccg tatgctaaaa
     2341 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2401 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2461 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2521 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2581 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2641 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2701 agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt
     2761 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2821 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     2881 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     2941 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     3001 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3061 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3121 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3181 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3241 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3301 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3361 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3421 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3481 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3541 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3601 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3661 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3721 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3781 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3841 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     3901 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     3961 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     4021 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4081 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4141 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4201 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4261 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4321 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4381 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4441 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4501 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4561 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat
     4621 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4681 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4741 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4801 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4861 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     4921 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     4981 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     5041 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5101 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5161 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5221 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5281 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5341 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5401 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5461 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5521 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5581 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5641 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5701 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5761 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5821 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     5881 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa
     5941 aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc
     6001 aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc
     6061 aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa
     6121 taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa
     6181 gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa
     6241 attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc
     6301 tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc
     6361 ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac
     6421 ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat
     6481 taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg
     6541 gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat
     6601 ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc
     6661 gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc
     6721 aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct
     6781 tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt
     6841 cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag
     6901 tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg
     6961 ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat
     7021 ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc
     7081 ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt
     7141 ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga
     7201 tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt
     7261 tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat
     7321 attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt
     7381 ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa
     7441 tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat
     7501 ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta
     7561 cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg
     7621 tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc
     7681 cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct
     7741 caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca
     7801 tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt
     7861 tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg
     7921 tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg
     7981 attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg
     8041 cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg
     8101 atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac
     8161 attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga
     8221 acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac
     8281 aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga
     8341 gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc
     8401 caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga
     8461 gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc
     8521 catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa
     8581 tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc
     8641 ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc
     8701 gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta
     8761 tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa
     8821 aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt
     8881 ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac
     8941 tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg
     9001 cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac
     9061 ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc
     9121 ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg
     9181 tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa
     9241 caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat
     9301 ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc
     9361 ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt
     9421 ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc
     9481 aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc
     9541 taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg
     9601 taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac
     9661 cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg
     9721 ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca
     9781 aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc
     9841 cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt
     9901 cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa
     9961 gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt
    10021 gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc
    10081 actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata
    10141 ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac
    10201 tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct
    10261 ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt
    10321 tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat
    10381 ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt
    10441 ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt
    10501 ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa
    10561 ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag
    10621 aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta
    10681 gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa
    10741 ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca
    10801 aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca
    10861 tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag
    10921 ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg
    10981 tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac
    11041 ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg
    11101 cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat
    11161 cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac
    11221 caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt
    11281 ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta
    11341 gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg
    11401 tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca
    11461 cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg
    11521 aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg
    11581 tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag
    11641 actggaactt tcccggattc taattcagcc atctcctgtc cgttaactac tacatattgt
    11701 ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct
    11761 tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc
    11821 aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt
    11881 ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca
    11941 ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca
    12001 ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat
    12061 ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcggtt
    12121 gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata
    12181 tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc
    12241 cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc
    12301 gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc
    12361 tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg
    12421 ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct
    12481 ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata
    12541 gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc
    12601 cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga catcgcatgt
    12661 ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc
    12721 tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc
    12781 ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg
    12841 tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg
    12901 tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg
    12961 gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat
    13021 tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc
    13081 tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag
    13141 cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact
    13201 ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca
    13261 tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt
    13321 gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga
    13381 ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag
    13441 atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat
    13501 gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga
    13561 agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg
    13621 ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta
    13681 atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt
    13741 aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc
    13801 aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca
    13861 agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga
    13921 ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct
    13981 ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta
    14041 ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt
    14101 tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg
    14161 cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct
    14221 tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt
    14281 taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt
    14341 taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac
    14401 ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatcta
    14461 ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc
    14521 cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag
    14581 ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa
    14641 attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc
    14701 ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat
    14761 tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgcaa aaatttccgt
    14821 cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta
    14881 aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg
    14941 ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt
    15001 acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag
    15061 ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc
    15121 tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat
    15181 tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc
    15241 gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg
    15301 agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg
    15361 tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag
    15421 aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat
    15481 agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag
    15541 taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat
    15601 tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg
    15661 tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga
    15721 ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc
    15781 catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat
    15841 tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt
    15901 gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat
    15961 ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag
    16021 catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta
    16081 gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat
    16141 agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca
    16201 gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa
    16261 aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc
    16321 ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg
    16381 tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat
    16441 agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaact
    16501 tcatctattc tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg
    16561 atcttttctg tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg
    16621 acataccatt cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag
    16681 gaggatagac ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg
    16741 attgctaaac gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat
    16801 tcaagggttt cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag
    16861 aatacctcat cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga
    16921 tagactgact ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc
    16981 aattttaacc ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca
    17041 atcaacaaaa caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg
    17101 atatcctttt tagttaaaaa acaccaatta tacaacattt attattaaaa ttaaattata
    17161 aaattttata acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac
    17221 tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta
    17281 ttcgtacaat taattcagat aacggcagcg cataggatgc caataacact tcctaaaata
    17341 gctcctccta aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc
    17401 aaacaagcaa ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct
    17461 actgataaaa aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt
    17521 gtgtaaagga caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg
    17581 ataaaaagca aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat
    17641 tctctagtat caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca
    17701 ttagagatat tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc
    17761 ttggcgaact tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc
    17821 ctccttttta agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa
    17881 caagaaactg aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag
    17941 aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa
    18001 aaatgcagta atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa
    18061 gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc
    18121 cccaccccaa tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc
    18181 taaactatct tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga
    18241 accgagcttt ttctcttcat tttttttgta atgataaatc atatacagaa ctgttcgact
    18301 tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat
    18361 aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga
    18421 tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt
    18481 gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa
    18541 ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt
    18601 tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa
    18661 ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata
    18721 tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag
    18781 taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg
    18841 ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt
    18901 acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga
    18961 aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact
    19021 actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc
    19081 gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg
    19141 atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg
    19201 attcctatta ctgtcatgag gacataactt agtatttttt ttgtttgccg attcacactg
    19261 gtctttttga aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg
    19321 caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag
    19381 ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa
    19441 aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa
    19501 aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta
    19561 atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt
    19621 catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag
    19681 aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag
    19741 ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa
    19801 gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag
    19861 ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac
    19921 tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt
    19981 tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca
    20041 tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta
    20101 gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt
    20161 cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag
    20221 tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc
    20281 aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat
    20341 gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg
    20401 tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc
    20461 tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact
    20521 tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat
    20581 ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct
    20641 gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa
    20701 taattgatat tttgggcacg catggctact aatccggaaa taaatggatc agatacacca
    20761 gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct
    20821 tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg
    20881 tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat
    20941 aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct
    21001 gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta
    21061 aataactttt tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga
    21121 tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt
    21181 tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt
    21241 atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc
    21301 cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg
    21361 aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag
    21421 aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg
    21481 gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc
    21541 aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg
    21601 gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa
    21661 aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt
    21721 aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta
    21781 gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga
    21841 aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc
    21901 tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg
    21961 caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg
    22021 gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta
    22081 tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca
    22141 gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact
    22201 ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa
    22261 tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc
    22321 atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag
    22381 tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact
    22441 tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg
    22501 aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac
    22561 cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa
    22621 acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa
    22681 ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct
    22741 aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac
    22801 ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca
    22861 ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc
    22921 gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca
    22981 aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg
    23041 attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta
    23101 ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta
    23161 tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct
    23221 tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca
    23281 atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata
    23341 aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc
    23401 cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga
    23461 atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat
    23521 tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc
    23581 cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc
    23641 taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata
    23701 cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg
    23761 agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac
    23821 tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct caactacatg
    23881 cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat
    23941 tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta
    24001 taaaataacc aacagcaaaa aatacaatga aaaacaaact tggaactaat gtatcattaa
    24061 taaatgttga aaaattcagc aactcattac tgtatgattt tagcagccca accaacatat
    24121 accctataaa agctaataag atggttagga atcctttaga atttttactc attcatagac
    24181 acttccttaa ataactttta ttatagaagg tattgtaaat aataatattg acaaaaagtg
    24241 actcaaaata aaaaaaaact tacattatac tggaatgaac tgtattgata gtgtacctaa
    24301 aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg
    24361 taatcaatta gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt
    24421 gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt
    24481 ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt
    24541 tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga
    24601 tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc
    24661 ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga
    24721 gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat
    24781 tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa
    24841 aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta
    24901 gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg
    24961 gccttttttt aatttcgtta tgtttagcat ataaaaatat tatagagttt acttacaatg
    25021 cttaaaacat atactgtata atctgatcaa ttttagccat gtcttgcaat ggtaagtttt
    25081 ctattttttt gagttttcgt tccttaaaat caagagattt taattgtgta ataattactt
    25141 gtccttttat atctagctct tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg
    25201 aagtaattgg acatatgacc gaaaacattg ttttacggtt aaattcataa cgagagacga
    25261 ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa tcaatccata
    25321 caatatcacc ttttttagga atataaattc tttcttctga cactataaaa tttctctccc
    25381 ttcaggagtt aaatcttccc attcgtcttt ctcataatac tcggcctctt ccccaccact
    25441 aaatggatct tcaatttttg gaactaacgt aattgttcca tcatcagaat acacaacgat
    25501 atattcttgg ttttctgaag gtttctttcc gttatcagaa ggtaaagtta ccactacaga
    25561 acttccttgt aatctagatt ttgtaacaag cattatcatc actcccttaa taaaagtata
    25621 ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt
    25681 ttaaagtata tcttattgga tataaataga aataaattgt agccagttca ttttttcttt
    25741 aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta
    25801 tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa
    25861 gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc
    25921 tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg
    25981 gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga
    26041 ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga
    26101 tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac
    26161 aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg
    26221 ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag
    26281 ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagtat
    26341 ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc
    26401 tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag
    26461 taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca
    26521 agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta
    26581 tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta
    26641 ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac
    26701 acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg
    26761 ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg
    26821 agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc
    26881 tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt
    26941 ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa
    27001 tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt
    27061 tatagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggt
//
LOCUS       DBNIBO010000019        27098 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.019, whole genome shotgun sequence.
ACCESSION   DBNIBO010000019 DBNIBO010000000
VERSION     DBNIBO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.019"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(233..1081)
                     /locus_tag="AC1Y77_000734"
     CDS             complement(233..1081)
                     /locus_tag="AC1Y77_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1574334.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="AC1Y77_000735"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="AC1Y77_000735"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIQ1574335.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="AC1Y77_000736"
     CDS             complement(3582..3761)
                     /locus_tag="AC1Y77_000736"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HIQ1574336.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /locus_tag="AC1Y77_000737"
     CDS             complement(3808..5631)
                     /locus_tag="AC1Y77_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1574337.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /gene="helD"
                     /locus_tag="AC1Y77_000738"
     CDS             5909..8188
                     /gene="helD"
                     /locus_tag="AC1Y77_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HIQ1574338.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="AC1Y77_000739"
     CDS             complement(8281..8928)
                     /locus_tag="AC1Y77_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574339.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="AC1Y77_000740"
     CDS             complement(9107..9826)
                     /locus_tag="AC1Y77_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574340.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="AC1Y77_000741"
     CDS             complement(9957..11108)
                     /locus_tag="AC1Y77_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIQ1574341.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="AC1Y77_000742"
     CDS             complement(11325..12065)
                     /locus_tag="AC1Y77_000742"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIQ1574342.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="AC1Y77_000743"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="AC1Y77_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIQ1574343.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="AC1Y77_000744"
     CDS             complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="AC1Y77_000744"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIQ1574344.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13676)
                     /locus_tag="AC1Y77_000745"
     CDS             complement(13026..13676)
                     /locus_tag="AC1Y77_000745"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIQ1574345.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="AC1Y77_000746"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="AC1Y77_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIQ1574346.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="AC1Y77_000747"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="AC1Y77_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HIQ1574347.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="AC1Y77_000748"
     CDS             complement(15119..15646)
                     /locus_tag="AC1Y77_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIQ1574348.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="AC1Y77_000749"
     CDS             complement(15826..16611)
                     /locus_tag="AC1Y77_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HIQ1574349.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /gene="accD"
                     /locus_tag="AC1Y77_000750"
     CDS             complement(16604..17473)
                     /gene="accD"
                     /locus_tag="AC1Y77_000750"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIQ1574350.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="AC1Y77_000751"
     CDS             complement(17474..18853)
                     /locus_tag="AC1Y77_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIQ1574351.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="AC1Y77_000752"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="AC1Y77_000752"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIQ1574352.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="AC1Y77_000753"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="AC1Y77_000753"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIQ1574353.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="AC1Y77_000754"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="AC1Y77_000754"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIQ1574354.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="AC1Y77_000755"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="AC1Y77_000755"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIQ1574355.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="AC1Y77_000756"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="AC1Y77_000756"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIQ1574356.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="AC1Y77_000757"
     CDS             complement(22811..23035)
                     /locus_tag="AC1Y77_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIQ1574357.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="AC1Y77_000758"
     CDS             complement(23088..24050)
                     /locus_tag="AC1Y77_000758"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIQ1574358.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="AC1Y77_000759"
     CDS             complement(24060..24500)
                     /locus_tag="AC1Y77_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1574359.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="AC1Y77_000760"
     CDS             24806..25771
                     /locus_tag="AC1Y77_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIQ1574360.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="AC1Y77_000761"
     CDS             complement(25828..26058)
                     /locus_tag="AC1Y77_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HIQ1574361.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="AC1Y77_000762"
     CDS             complement(26144..27031)
                     /locus_tag="AC1Y77_000762"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIQ1574362.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
BASE COUNT         7514 a         5951 c         4673 g         8960 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttt
//
LOCUS       DBNIBO010000020        26602 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.020, whole genome shotgun sequence.
ACCESSION   DBNIBO010000020 DBNIBO010000000
VERSION     DBNIBO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.020"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            147..1016
                     /locus_tag="AC1Y77_000763"
     CDS             147..1016
                     /locus_tag="AC1Y77_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1574363.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="AC1Y77_000764"
     CDS             1225..2250
                     /locus_tag="AC1Y77_000764"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIQ1574364.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="AC1Y77_000765"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="AC1Y77_000765"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HIQ1574365.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="AC1Y77_000766"
     CDS             3333..5036
                     /locus_tag="AC1Y77_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1574366.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="AC1Y77_000767"
     CDS             5038..7071
                     /locus_tag="AC1Y77_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1574367.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="AC1Y77_000768"
     CDS             7082..7522
                     /locus_tag="AC1Y77_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1574368.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="AC1Y77_000769"
     CDS             complement(7722..8849)
                     /locus_tag="AC1Y77_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574369.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="AC1Y77_000770"
     CDS             complement(8852..9529)
                     /locus_tag="AC1Y77_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1574370.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="AC1Y77_000771"
     CDS             9793..11559
                     /locus_tag="AC1Y77_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HIQ1574371.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="AC1Y77_000772"
     CDS             11574..12011
                     /locus_tag="AC1Y77_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1574372.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="AC1Y77_000773"
     CDS             12024..13175
                     /locus_tag="AC1Y77_000773"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HIQ1574373.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="AC1Y77_000774"
     CDS             complement(13237..13935)
                     /locus_tag="AC1Y77_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIQ1574374.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="AC1Y77_000775"
     CDS             complement(13928..15280)
                     /locus_tag="AC1Y77_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HIQ1574375.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="AC1Y77_000776"
     CDS             complement(15503..16903)
                     /locus_tag="AC1Y77_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIQ1574376.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="AC1Y77_000777"
     CDS             17064..17801
                     /locus_tag="AC1Y77_000777"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIQ1574377.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="AC1Y77_000778"
     CDS             17926..18405
                     /locus_tag="AC1Y77_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HIQ1574378.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="AC1Y77_000779"
     CDS             18402..19169
                     /locus_tag="AC1Y77_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HIQ1574379.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="AC1Y77_000780"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="AC1Y77_000780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIQ1574380.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="AC1Y77_000781"
     CDS             complement(20072..20674)
                     /locus_tag="AC1Y77_000781"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1574381.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="AC1Y77_000782"
     CDS             20780..21010
                     /locus_tag="AC1Y77_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574382.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="AC1Y77_000783"
     CDS             21001..21249
                     /locus_tag="AC1Y77_000783"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1574383.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="AC1Y77_000784"
     CDS             21355..21939
                     /locus_tag="AC1Y77_000784"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIQ1574384.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="AC1Y77_000785"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="AC1Y77_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIQ1574385.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="AC1Y77_000786"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="AC1Y77_000786"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIQ1574386.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="AC1Y77_000787"
     CDS             23840..24019
                     /locus_tag="AC1Y77_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574387.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="AC1Y77_000788"
     CDS             24041..25066
                     /locus_tag="AC1Y77_000788"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIQ1574388.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /gene="glyA"
                     /locus_tag="AC1Y77_000789"
     CDS             25241..26485
                     /gene="glyA"
                     /locus_tag="AC1Y77_000789"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIQ1574389.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
BASE COUNT         9069 a         4698 c         5433 g         7402 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa tt
//
LOCUS       DBNIBO010000021        26567 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.021, whole genome shotgun sequence.
ACCESSION   DBNIBO010000021 DBNIBO010000000
VERSION     DBNIBO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.021"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(118..708)
                     /locus_tag="AC1Y77_000790"
     CDS             complement(118..708)
                     /locus_tag="AC1Y77_000790"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HIQ1574390.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1905
                     /locus_tag="AC1Y77_000791"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="AC1Y77_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="AC1Y77_000792"
     CDS             1898..2059
                     /locus_tag="AC1Y77_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574391.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2543..3139
                     /locus_tag="AC1Y77_000793"
     CDS             2543..3139
                     /locus_tag="AC1Y77_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIQ1574392.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            3120..3566
                     /locus_tag="AC1Y77_000794"
     CDS             3120..3566
                     /locus_tag="AC1Y77_000794"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIQ1574393.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            3909..4232
                     /gene="relB"
                     /locus_tag="AC1Y77_000795"
     CDS             3909..4232
                     /gene="relB"
                     /locus_tag="AC1Y77_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIQ1574394.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            4220..4300
                     /locus_tag="AC1Y77_000796"
                     /pseudo
     CDS             4220..4300
                     /locus_tag="AC1Y77_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
     gene            4552..4779
                     /locus_tag="AC1Y77_000797"
     CDS             4552..4779
                     /locus_tag="AC1Y77_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIQ1574395.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4795..5961
                     /gene="hemW"
                     /locus_tag="AC1Y77_000798"
     CDS             4795..5961
                     /gene="hemW"
                     /locus_tag="AC1Y77_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIQ1574396.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6196..7239
                     /gene="hrcA"
                     /locus_tag="AC1Y77_000799"
     CDS             6196..7239
                     /gene="hrcA"
                     /locus_tag="AC1Y77_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIQ1574397.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7333..7896
                     /gene="grpE"
                     /locus_tag="AC1Y77_000800"
     CDS             7333..7896
                     /gene="grpE"
                     /locus_tag="AC1Y77_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIQ1574398.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7947..9776
                     /gene="dnaK"
                     /locus_tag="AC1Y77_000801"
     CDS             7947..9776
                     /gene="dnaK"
                     /locus_tag="AC1Y77_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIQ1574399.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9927..11093
                     /gene="dnaJ"
                     /locus_tag="AC1Y77_000802"
     CDS             9927..11093
                     /gene="dnaJ"
                     /locus_tag="AC1Y77_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIQ1574400.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11461..12198)
                     /locus_tag="AC1Y77_000803"
     CDS             complement(11461..12198)
                     /locus_tag="AC1Y77_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574401.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12200..13702)
                     /locus_tag="AC1Y77_000804"
     CDS             complement(12200..13702)
                     /locus_tag="AC1Y77_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIQ1574402.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13961..15955
                     /gene="uvrB"
                     /locus_tag="AC1Y77_000805"
     CDS             13961..15955
                     /gene="uvrB"
                     /locus_tag="AC1Y77_000805"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIQ1574403.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            15965..18781
                     /gene="uvrA"
                     /locus_tag="AC1Y77_000806"
     CDS             15965..18781
                     /gene="uvrA"
                     /locus_tag="AC1Y77_000806"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIQ1574404.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19044..20309
                     /locus_tag="AC1Y77_000807"
     CDS             19044..20309
                     /locus_tag="AC1Y77_000807"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HIQ1574405.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20313..21044
                     /locus_tag="AC1Y77_000808"
     CDS             20313..21044
                     /locus_tag="AC1Y77_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HIQ1574406.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21179..22063
                     /gene="rapZ"
                     /locus_tag="AC1Y77_000809"
     CDS             21179..22063
                     /gene="rapZ"
                     /locus_tag="AC1Y77_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIQ1574407.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22060..23058
                     /locus_tag="AC1Y77_000810"
     CDS             22060..23058
                     /locus_tag="AC1Y77_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIQ1574408.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23092..24027
                     /gene="whiA"
                     /locus_tag="AC1Y77_000811"
     CDS             23092..24027
                     /gene="whiA"
                     /locus_tag="AC1Y77_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIQ1574409.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24595..25251
                     /locus_tag="AC1Y77_000812"
     CDS             24595..25251
                     /locus_tag="AC1Y77_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574410.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25430..25995
                     /locus_tag="AC1Y77_000813"
                     /pseudo
     CDS             25430..25995
                     /locus_tag="AC1Y77_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8951 a         4366 c         5614 g         7636 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     2461 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     2521 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     2581 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     2641 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     2701 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     2761 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     2821 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     2881 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     2941 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     3001 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
     3061 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaatcaag agcaattgat
     3121 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
     3181 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
     3241 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
     3301 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
     3361 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
     3421 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     3481 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     3541 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     3601 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     3661 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaatt
     3721 ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt agttggctac
     3781 ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat ttactacaag
     3841 gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa aatcacatca
     3901 ggaaatgcat gagattcttt aagagaggca attatagggc tatgattacg gttcgtgtaa
     3961 gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt actttatctg
     4021 aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt caaacggctg
     4081 aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg gaagaatcct
     4141 taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga aattcagaag
     4201 cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat tcgttgttat
     4261 cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt atttttacaa
     4321 aaaaataata attggaatct agttaaaact cttgaagtga gagatttgac tagatttttt
     4381 tatgaataaa ataaggacaa aattgtttag gaagaatcat gagtgtaaaa aaatcatcca
     4441 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
     4501 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
     4561 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
     4621 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
     4681 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
     4741 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
     4801 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
     4861 catatctgct attactgtga ctttaataaa gtttttttag aagggcagcc agtagatgag
     4921 tatgtggaaa tgctgctgaa tgaaatgaga ttaatgctgg aaagtcatcc tacagcgaat
     4981 ttggaaacac tttatgtagg aggcggaaca ccaacttctt tatctgctaa acaacttgat
     5041 cgactattat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
     5101 gaagcgaatc ctggtgattt gacccgtgaa aaacttcaag tgatgaaaaa ctatggcgtc
     5161 aatcgtttat ccatgggagt gcagacattt gacaatcgct tattgaaaaa aattggacgt
     5221 aaacatactg cagaagatgt gtatcaaaca atgcaatttt tagaagcaga aaattttacc
     5281 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatcgggat
     5341 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
     5401 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
     5461 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
     5521 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
     5581 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
     5641 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
     5701 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
     5761 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
     5821 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
     5881 gaagaagacc gtgtttattt gacaccaaga gggacttttt tggggaacaa tgtgtttgag
     5941 cgctttttat ttgaaaaata gtttgaagaa tatttgtttt ttatcgaaac ttgaaaagaa
     6001 aaagtttaga aagtttgaca atgaggtatt gaagtattga tataactgga tttacatcaa
     6061 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
     6121 ggagaagggc ttcttgctat atttataagt gaattagcac ttagaatata tgagtgctaa
     6181 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
     6241 cactatacga gtagtggcgt gcccgttggt tccaaaacat taatgtctga gggagtcaag
     6301 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
     6361 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
     6421 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
     6481 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
     6541 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
     6601 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
     6661 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
     6721 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
     6781 cttcgtacgg agattccgat gattcttcat aaatatttcc agacgacaga agggatgtct
     6841 cagttatttg acaccgttct tggtcatgtt tttgaagaaa aggtttatgt cagtggacaa
     6901 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
     6961 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
     7021 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
     7081 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
     7141 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
     7201 gctgattact accgctcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
     7261 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
     7321 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
     7381 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
     7441 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
     7501 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
     7561 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
     7621 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
     7681 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
     7741 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
     7801 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
     7861 gtgttaagac caactatggt cattgttgca caataattgt gcggatataa aacttacact
     7921 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
     7981 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
     8041 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
     8101 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
     8161 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
     8221 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
     8281 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
     8341 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
     8401 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
     8461 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
     8521 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
     8581 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
     8641 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
     8701 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
     8761 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
     8821 gtcaagcgtt aaaagatgca ggtctgtcac aatcagaaat cgacgaagtt attttagtcg
     8881 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
     8941 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
     9001 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
     9061 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
     9121 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
     9181 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
     9241 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
     9301 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
     9361 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtgaaa gatgcagaag
     9421 caaacgctga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagacg
     9481 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
     9541 aagtgaaaaa agcagaaaca gctcgtgacg aattaaaagc cgctgttgaa gcaaacaaca
     9601 tcgaggaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
     9661 aactatacga acaagctgca caacaacaag ctcaagaaaa tccagaagca gctcaaggcg
     9721 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
     9781 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
     9841 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
     9901 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
     9961 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    10021 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    10081 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atacgaccaa tacgggcatg
    10141 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    10201 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    10261 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    10321 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    10381 aaatctgtca tacttgtggt ggaaacggtg caaaaccagg cacacaacca actacttgtc
    10441 ataaatgtca tggtgcaggt acgatcaatg tagaacgcca aacgccactt ggacgagtga
    10501 tgagccgtca aacttgtgat gtttgtcatg gcacagggaa agaaatcaaa gaaccttgtc
    10561 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    10621 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    10681 gcggaccatt tggtgatttg tatgtggttt ttcgtgtaga agaaagtgat atctttgatc
    10741 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    10801 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    10861 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    10921 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    10981 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    11041 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    11101 agtaaaaata taaagtagac ttgcccaaat tttctatttc tagtaaaaac gctaagtcat
    11161 taataattaa aaaccagacg aacaatcata cgttcgcctg gtttttttgt agttgtcatg
    11221 tttgtaccaa caaaaaagcc agctttagaa taagataatc atcatagttt ctgaaggtgg
    11281 tatcgactat gtacttgata tcgtcaatga accgatggta ctttcttatg cgatggaaca
    11341 ggtagagcga ttgcttgttt gtgcaggaga acaagcgatg cggctggtac agttaagtga
    11401 aaaaacaaga aactaaaaat gcccggtcgt catgaagcga ccgggcattt ttggttttta
    11461 ttaaatattt aatactttat tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa
    11521 gatttgttga ggcgttccgt cttctaaaaa gtttccgtcg tcaatgaaca tgacacgatt
    11581 ggccacttct ttagcgaagc ccatttcgtg cgtaacgata accatcgtca taccttgttc
    11641 tgctaacttt ttcatgacgt tcaagacatc accgaccatt tcggggtcga gtgcagatgt
    11701 aggctcatca aaaagcatga tgtcagggtt catcgctaga gcacgagcaa tagccacacg
    11761 ctgcttttgt ccaccggaaa gcatttcagg atacatatct tttttatctt ctagaccaac
    11821 tgtctgcagc aatttgattg cttttttctc tgcttcttct ctagattctt tcttcaggtc
    11881 catcggtgct aaaatgatgt tttccaaaat agaaagatga gggaataggt taaagtgctg
    11941 gaataccatt ccgatatgtt gtctcaccaa gttgatatct gtgcttttat ccatcaagtg
    12001 ggcgccgtca ataataattt caccgtcaga aggttcttct aaacgattca agcaacgcaa
    12061 aaatgtactt tttcctgaac ctgaagggcc aatgatacag acgacatctc cttcgttgat
    12121 ggacacattg atgtcgttta atactgtgtt atcgccgtat tttttgacta aatgctcaac
    12181 taagattttt tctgccatct tacttcacct tcttttctaa aacttttgct aattttgtca
    12241 atgctgtgat caaaatcaag tacatgatcg caatgatcaa gtaaacatac gtactttgtg
    12301 ttgtacgtgc aacaatgatc ttaccagttt gcagcaattc gactacaccg attgcggaaa
    12361 tgatcgttgt gtcttttaat gagataacga actgattgac gaatgatggg atcatgatgc
    12421 gtatcgcttg cggcaaaatg attttttgca ttgtccgatt ataagaaaga cctaaacttc
    12481 tggaagcttc catttgtccg acaggaacgg cgttgatccc gcctcgaaca atctctgcga
    12541 tataagcact tgcgttaaga gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa
    12601 atccaacgat accaggcaac ccaaagaaaa taaagaacgc aagaaccatc attggaattc
    12661 ctcggatgat atcgacataa atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac
    12721 tgaataatcc aaaaatcaca ccgattacta tagccaaagc aaaggagatc aaagttagga
    12781 gaattgttct ccaaagtcct ttcaacaatg cttgatagtt gttttggata agtccaagaa
    12841 atgttgattc atcagcagtg gaagactcac tggaagtttc tgaaccatcc gctacatatt
    12901 ctttaataat cttatcgtat tcgcctgttc tttttaattc tttcaatcct tcattgaaca
    12961 tattaagaag ctcaggattt tgtccttttt taacggcaaa tgcatatttt ccgccagatt
    13021 cttttttgat cggtgtttcc aattcttgtc cttgtttgat cgcataaccg attaccggat
    13081 aatcatccat cattgcatcg attgcgccaa cttttaaccc gtcgtacaat tgatcagcag
    13141 catcaaaata tttgatagaa tagccatatt cagattcatg gtcgcttaaa aattcagcac
    13201 tttcggtacc aacttttgct ccgactgttt ttcctttcaa atcttcataa gacttgatcg
    13261 aagtatctcc ttttttgaca gctaattgga tcccgctatc aaagtaagaa tcagaaaaat
    13321 catagctttt ctttcgttca tctgttacag tcattccggc aatcattccg tctgcttgtc
    13381 ctgattccaa agcttgcaaa gcagcagaga aaccacggta gtccattcgg atgttaaatc
    13441 cttgcagttt ggcgatgttt tccatcaatt ttacatcgat gccttcgtac tcgccctttg
    13501 cgttttgaaa ttcaaatgga gcaaaagcag agtcactggc aattacgtat acgtcttttt
    13561 tgggctctat tttttgcatt gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag
    13621 cttgtgcaac atgtccaaac gcaagtgtca caagtcctgt cattaagaaa acaaatgagt
    13681 gaaataaatg ctttcttttc ataatcaacc tcctaaattt ccaatatttt attattatag
    13741 gcgaaaagtg ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa atgcgagttt
    13801 ctaacatttt atgttataaa acgtaacatg cataataaga taaaaaagtg gcgaaagtat
    13861 gttcggtgtg gtataatttt ttagtaagga atgcctttgc tttccttttc tttttattgg
    13921 tcgtaaaatg aaaaggaatg aatgaaaagg aggacgaaga atgattgaga gagatacctc
    13981 ccgacacttt gagcttgtct cgaaatacca accggctggt gatcagcctg aagcgattaa
    14041 ccaattagtg gatggcgtag tcggcgggaa aaaagcacaa atcttattag gtgcgactgg
    14101 aacagggaaa acatatacga tttccaatct tattgagaaa gtgaataaac caacactaat
    14161 tattgcccat aacaaaacat tggctggtca gctgtacggg gaattcaagg aatttttccc
    14221 taacaatgct gttgaatatt ttgtttctta ttatgattat tatcagcctg aagcttatgt
    14281 gccttcaagc gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt
    14341 acgacattcg gctactagtt cattattaga aagaaatgat gtgatcgtca ttgcatccgt
    14401 ttcatgtatt tttggattag gttcgccttt tgagtaccaa aaacaagtag tttcgattcg
    14461 acagggagca gaactagatc gcaatcagtt gattcgtgac ttagtcagta tccaattcga
    14521 aagaaatgat atagattttc agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat
    14581 ttttccggct tcaagagatg aacgtgcctt acgtgtggag ttttttggag atgaggtcga
    14641 acgtatccga gaagtcaatg cactgacagg tgaagtttta ggagaaacgg aacacgtggc
    14701 aatcttcccg gcgactcact ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa
    14761 tatcaaagcg gaattagaac agcgtttaac tgtcttgcga aatgaaaaca aacttctgga
    14821 agctcaacgt ttagaacagc gaaccaatta cgatattgaa atgatgttag aaatgggcta
    14881 cacgtcggga attgaaaatt attctcggca tatggacgga cgtaaagaag gtgaaccgcc
    14941 ttatacgcta ttggatttct ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt
    15001 aaccatgcct caagtcagag gaatgtataa tggtgaccga gcgagaaaac agatgctagt
    15061 agattacggt ttccgtttgc catctgcttt agacaatcgg ccacttcggt tagaagagtt
    15121 tgaaaaacat gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat atgaacatga
    15181 acaaacagat accgttatcc aacaaatcat ccgtccgaca gggctgcttg acccagtgat
    15241 cgaagttcgt ccaatcatgg gacaaatcga tgatctagta ggcgaaataa atgaacgagt
    15301 cgaaaaagac cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag aagacctcac
    15361 agattatttc aaagaattag ggatcaaagt caaatatttg catagtgata tcaaaacgct
    15421 ggaacgaaca gaaatcattc gagatcttcg attaggtgaa tttgatgtgt tagttggaat
    15481 caatttgctt cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta tcttggatgc
    15541 ggataaagaa ggctttttac gtagtgaacg ttccttagtc caaacgattg gacgagctgc
    15601 tcggaatgaa gaaggtaaag taatcatgta cgcagacaaa gtaacagatt ctatgcgtct
    15661 agcgatggat gaaacatcaa gacgccgtac gattcagcag aagtacaatg aagaacatgg
    15721 gatcgttcca aaaacgatca ttaaagaaat ccgagatttg atttctatta cgaaagaatc
    15781 ggaagatgat acaaaagaag cggtacaagt ctcgtatgaa gaaatgacga aagaagaaaa
    15841 agatacgtta ttgatgaaat tagaaaaaga aatgaaaaat gcggcgaaag cattggattt
    15901 tgaaacagcc gctaatgtgc gagatatgat tttagaatta aaagcttcaa agtaggagga
    15961 caacatggct aacgataaaa ttacgattca cggtgcacgt gcccataact tgaaaaatat
    16021 agatgtaacg attccacgag ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa
    16081 aagttcccta gcatttgata cgctgtacgc tgaagggcag cggagatacg tagagagcct
    16141 ttctgcttat gctcgtcagt ttttaggaca aatggataag ccggacgtag atagcatcga
    16201 cggattgagt ccggcgattt ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac
    16261 agtaggtact gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca
    16321 tccgatttgt cccaacgatc atatcgagat taccagtcaa tcagtagatc aaatggcaga
    16381 tcaagtaatg gctttgcctg agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa
    16441 gaaaaaagga caacataaaa aaatattcga acgaatccaa aaagagggat acgtccgcgt
    16501 tcgagtcgat ggtgagatct atgatttaag tgaagtgcct gagctggaga aaaacaaaaa
    16561 acatgatatc gcgatcgtca tcgaccggat cattgtgaaa gaagggattc gttctcgtct
    16621 atttgattct ttagaggcgg ctctgcgatt ggcagacggc tatgctctgg ttgacgtgat
    16681 cggtgaagaa gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg gatttactgt
    16741 tggcgaacta gaaccaagac tgttctcatt caatgcgcct tttggagctt gtccagactg
    16801 tgatggttta ggtgtcaaat tagaagtcga tcaagattta gtgattccag atagaacgaa
    16861 gacacttagt caaggtgcta tcgttccatg gaatccaatc agctctcagt attatcctca
    16921 aatgttagcg caggcttgtg aaagtttcgg tatcgattta gatacaccat tcgaagaatt
    16981 accggaagag catcaggata tcatcttgaa tgggacagat gatcttttcc atttccatta
    17041 tgaaaatgat tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa
    17101 tatcgagcgt cgttatcatg atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta
    17161 tatgacagaa ttaacttgtc agacatgtaa aggttatcga ttgaatcccc aagctctatc
    17221 tgttcaaatc aatggaacga atatcgggca aacaaatgag ttgtctattc aaaaagcagt
    17281 agatttcttc tcaaacttaa cgttatctga acaagagaaa gtaattgcca aaccgatttt
    17341 aaaagaagta aatgacagac tttcattctt ggaaaatgtg ggactagact atctaacttt
    17401 gagtcgcgct tccggaacat tatctggggg agaagcgcag cgaatcagat tggctacaca
    17461 aattggatcg aatctttctg gagttttgta tattttagat gaaccatcga tcggattgca
    17521 tcaacgcgac aacgatcgac tgattggctc attaaagaaa atgcgtgatc ttggcaacac
    17581 cctcgttgta gtcgaacatg atgaagatac gatgtgggca gcagattatc tgattgacgt
    17641 cggacctggg gccggagaac aaggcggaca aatcgtggct gcaggcactc ctgaagaagt
    17701 agctaatgat gaaaattctt tgacagggaa atatctttca gggaaaaaat caatccctgt
    17761 accaaaagaa cgccgtaaag gtaatgggaa agcaatcaag atcactggtg cttcagaaaa
    17821 caatctgaaa aatattagtg ttgaatttcc attaggagaa tttgtagcag tcacaggtgt
    17881 gtctggttct ggaaagagta cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa
    17941 attaaataag aattctgcta agccaggtaa attcaagaca atttccggct acgaaagtat
    18001 cgaaaagatc atcgatatcg atcaaagccc aatcggccgg acgccgagaa gtaatccagc
    18061 gacttataca agtgtatttg atgatatccg tgggttattt gctcaaacga acgaggcaaa
    18121 aatgcggggt tataagaaag ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc
    18181 ttgtcgcggg gatggaatta ttaagatcga aatgcacttt ttgcctgatg tctatgttcc
    18241 ttgtgaagta tgtcatggca aacgatataa ctctgaaaca ttagaagtgc attacaaagg
    18301 aaaaagcatt gctgatattt tggaaatgac agtagaagat gctgtagaat tcttcaagca
    18361 cattccaaag attcatcgca aactgcaaac gattgttgat gttggcttag gttatgtgac
    18421 gatggggcaa ccagcaacga cattgtccgg tggtgaggca caacggatga aacttgccag
    18481 tgaattgcac aaaatctcta atggaaagaa tttctatata ctagatgaac caacgacagg
    18541 acttcatagc gatgacatcg cccgcttgtt gcatgtatta caaagattag tagatgctgg
    18601 taacacagtt ttagtgattg aacacaatct agatgtaatc aaaacagcag attatatcat
    18661 tgatttagga ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg gaacaccaga
    18721 agaaatcatt aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa taatggtata
    18781 aagaggatag atagctgaga tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt
    18841 attaatatca gtgttctaga atcattacat ggtagttctt tctacactga ttggtataga
    18901 caaaaacttc ctataagttc aatccgttca aaaaagaatt gagcaatcgg aggagtttcc
    18961 tttgattgct tttttcttta tggtatgata ttccttgtgt agttggaaaa gaaaaacaag
    19021 agattatgag taagtgagag ataatgatga acagtattga aaatgaagaa tttatcccta
    19081 tattattagg aagcgacatg aacgtttatg gtatggcacg ttcttttaat gaagcatacg
    19141 gcaaaatctg ccaagcatat gcttcggatc agttagcccc tacccgctac agtaaaattg
    19201 tcaatgtaga agttattcca ggatttgata aagaccctgt gtttatcgag acgatgcttc
    19261 gtttggcaaa agaaagatat tctgataaga gtaaaaaata tcttttgatt gcttgtggtg
    19321 atggatatgc agaattgatc tcccaacata aacaagagct gtcagaatat tttatctgtc
    19381 catatattga ttattcttta tttgaaagac tgatcaataa agtaagcttt tatgaagttt
    19441 gtgaagaata tgatcttccg tatcctaaaa cattgatcgt cagagaagaa atgttagtaa
    19501 atggacattt ggaacaggaa ttgccttttg aattcccagt tgctttgaaa cctgcaaaca
    19561 gtgtggagta tctttctgtc caatttgaag gaagaaaaaa agcttttatt ttagagacta
    19621 gagaagagtt cgatttaatt ttaggccgga tatacgaagc cggttataaa agtgaaatga
    19681 ttgtacaaga ttttatcccg ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag
    19741 atgaagatca tcaagttcgg atgatgtgtc ttggtcatcc actgttagaa gatcctacac
    19801 cagcatcgat tggtaattac gttgtgatca tgccagatta taacgaaaaa atctatcaaa
    19861 ccattaaggc atttttggaa aaaatcgaat atacaggctt tgccaatttc gatatgaagt
    19921 atgatcctag agatggagaa tataaattat ttgaaatcaa tttgagacaa ggtcgtagca
    19981 gtttctttgt gacattgaac ggcttgaatc tggctcgttt cgtcacagaa gatcgagtgt
    20041 ttaacaaacc ctttgttgaa acgacctatg gaacgaatca atcagataaa gcaagattat
    20101 ggatgggtgt gcccaaaaaa atctttttag aatatgctcg ggaaaatgaa gacaaaaaac
    20161 tagctgagca aatgatcaaa gaaaatcgtt acggtactac tgtgttttat gaaaaagacc
    20221 ggtcaatcaa gcgctggttg ttgatgaaat atatgttcca taactatatt ccaagattca
    20281 aaaagtattt tcatgtgaaa gaaggctagt tcatggagaa ttttttcagt attttaggcg
    20341 gaatgggcac gatggcgaca gaaagttttg ttcgcttgat caaccaccga acgaaagcta
    20401 caaaagatca agaatatttg aattatgtct tattcaatca tgcaacagtt cctgatcgta
    20461 cagcttatat tttagatcga tcagaagaaa atccaatgcc gtttctgctg gatgatattg
    20521 agaaacaaaa tttattgcag ccgaacttca ttgtgttgac ttgtaacacc gcacactact
    20581 tttttgaaga attacaagcg gcaacggata ttcctatcct tcatatgcca agagaagcag
    20641 caaatgaact tgtccgtcag catacgacag ggagagtagc gattctagga acagaaggaa
    20701 gtatgaaagc tggaatctat gaacgcgaag tcaaaaatct tggattcgaa acagtgattc
    20761 ctgatactgt tcttcaagaa aaaatcaatt atctgatcta tcatgagatc aaggaatcgg
    20821 attatttgaa tcaagagcta tattacgaaa tcttagaaga agcggtagag cgcctaaact
    20881 gtgaaaaagt tatcttaggt tgcacagaat tgtcattgat gcatgaattt gctgaagata
    20941 accattatcc agtgatcgat gcccaatcta ttttagcaga tcggacgatt gaacgagcgc
    21001 tagctgaaag aagcaaggca ttggatacag catcggaaaa gtaaaaaaat tggatttggg
    21061 gcaaatatcc taaaagatat ttggtccaaa tctttttgta aaagaaaaag aaaactggta
    21121 atgatacgcc cggaatgata taattttact atgcacaatg tgagaggagt tcgtaattat
    21181 gacagataat ttgcaattag tcatcataac aggtatgagc ggtgcaggga aaacagtagc
    21241 tatccaaagt tttgaagata tgggctattt ttgtatcgat aatatgccgc ctcgattgat
    21301 tccaaaattc tgggaattaa tcaaagaatc aggcaaagtc acaaaaatgg ctttaggagt
    21361 ggatttacgt tctcgttcct tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa
    21421 tacgaacttc atcgacactt ctatcttatt tttagatgct tcagacgaag aactagtggc
    21481 tcgctacaaa gaaacaagac gagcacatcc aatggcgatg gacggtttga tcactgaagg
    21541 gatacgtaaa gaacgggcga tactagacga cttgaaagcc caagcttccg tgatcattga
    21601 tacgacgaca ttgacaccac gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa
    21661 gggagaccaa ggatttcgga tcgaatttgt atcatttggt tttaaatacg ggttgccgat
    21721 cgattcggat attgtgatgg atgtgcgttt tttgcccaac cctcattata tcccggaact
    21781 aagaccattg accggaaaag atcagccagt ctatgattat gtgatgtcct ttgatgaaac
    21841 gcaatcattc taccataaat ttttggatct tctggaaact gtattgcctg gatacattaa
    21901 agaaggaaaa agcagcttga cagttgcaat cggctgtaca ggcggtcaac accgttctgt
    21961 ggcattgact gaacgtttgg caaatgcttt agggaaaaac tataaagtca atgtgacaca
    22021 tcgggacaag gataaaagga aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg
    22081 ataagaaaac caaagatcgt agtggtcggc ggtggcactg gtctgccagt tatcctgaaa
    22141 agtttgcgaa atcaaggagc agatatcaca gcagtagtga ctgttgcaga tgatggggga
    22201 agcagcggtg caatcagaga atcgatcgct atggctcctc ctggtgactt gagaaatgtc
    22261 ttagtggcgt tatcggatat gccacaattc tatgaagata ttttccaata ccgtttcaaa
    22321 aaggaagatc agtttttagc caatcatacg atcggtaatc tgatcattgc cgcagtttcc
    22381 gagatgcggg gaagcactta tgaggcgatt caattattag caaaaatgat gcatgtagat
    22441 ggacatgttt atccttcttc agaaactcct ttgaccttgc atgctgtctt taaagacgga
    22501 acgactgcag taggagaatc taaaatcgcg atcgaccgta aaaccattga ccgtgtgtat
    22561 gtctgcaaca caaatgataa aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt
    22621 atggacgccg acatggtcgt tttgggaccc ggaagtttat tcaccagtat ccttccaaat
    22681 ttagtgatac cagaaatcgg cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc
    22741 tgtaatatca tgacccaaaa aggggaaacg gaacatttca cagatgccga tcatatacgt
    22801 gttttacaca agcatttgaa tcagcctttt atcgatacag tattggtcaa tacggaaaaa
    22861 gtgcctgaag attatatgga cccagagatc tatgatgagt atctagtaca agtcgcacac
    22921 gacttcaacg gattgagaaa tgaaggatgt cgagtgattt ccaccgactt tttggaattg
    22981 aaagatggtg gtgtcttcca cgatggagac aaagtagtag aagaattgtt ccgtttagtt
    23041 ttcggttcaa aatattaagc aatttattgt tggagaaagg aggagaatag gatgtcattt
    23101 gctgcagatg tgaagaaaga actaactggt ttggaagttc atcgagaaca tgcgaaagcg
    23161 gaattagctg ctttgctgcg aatgaacggc tctttaggta ttgtgaacca gcagtttatt
    23221 ttgaacgtcc aaacagaaaa tgctgcaatt gccagacgta tctattctct tttgaaagat
    23281 cattatcagg tacgaagtga gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat
    23341 gtctatatcg tccggttgaa gcaaggcaca aaagatgtgt tgatggatct tgatatcatg
    23401 gatggtatga tgttcaatgg acacgtttcg gacgagatta tgggaaattc gcaaaaaatg
    23461 cgttcttact tacgtggtgc ttttatggcc tcaggttcag tcaataatcc ggaaacgagc
    23521 cgttatcatt tggaaatatt ttctatttat gaacagcata accaagacat ttgcgatatg
    23581 ttaaattatt acggtctaca tgctcgaacg cttgaacggc gcaatggcta tatttcttat
    23641 ttaaaaggag cagaaaaaat tgctgatttc ttaacattga ttggtgcaac caactcaatg
    23701 ctccgatttg aagatgttcg aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc
    23761 aattgtgaga cagcaaatct gaacaagacg attgatgcgg cttctaaaca aatcgaaaac
    23821 atcgaattga tcgaagcaaa ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc
    23881 gcagaacttc ggcttcaaca tccagaggtc agcttgaaag aattagggga aatgattccc
    23941 tcaggtgcga tatcaaaatc gggaattaat caccgaattc gcaaaataaa tgaatttgcg
    24001 gaaaaactgc gtgaagaagc agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga
    24061 tgcgaatgta tcagtttttt ttaaaagtta tacttgcttt tcttccgtca aactataatt
    24121 ccatctttac tggagtaaca taatttggaa aggcttctat tgtttttaat acaatcgtat
    24181 aaaaagcggg gagacaactg aatgaagatt ttagttttgt acataaattt ttgcttaata
    24241 tagaagtcct gatatagata agatcaagta tttatctata actggcctga ttaattaaaa
    24301 aactggaagg aactgctcga tcatccttga gaactgctct tcttacttct ggtcaagatt
    24361 ctcgtggttg gtggataagg atggttgata agttcattgg attagctagt cgaaaacaac
    24421 caggtgtaat taatgtagaa gttttaacaa ggggatggag tgatttattc agtgataatc
    24481 ataagttcat aaatagtaat tgagatgtat ggttggaata cgttatacct gaacttgaac
    24541 taccaagatt tggtttagaa tttgagaatc gatgtttata aagggagggc aaaaatgcgt
    24601 ttttttacaa cttttactat cattatgatt gcagtacttt ttatttttct agatatagca
    24661 aagcgaaata cagcattttt attatatcgg gttttacttc gagcaggtct cataacattt
    24721 atatcgatcg ttggattttt tctattcaca gttattgtat ttatttggag aactccagca
    24781 ccaccattgc cagagattac atatggagaa tttcctttta gacttgagta tgaattgaat
    24841 gaagaattac atgtgataga agacacgtta atagttgaat ttgatggatt tggaatgaac
    24901 gaaggcatag ggagatatcg tagatggaca agtcgtttag ccagcggaga agatttagtg
    24961 ttgctgttag aagttagtga taataagcaa atattttatt ttccaggacc tgctaattat
    25021 tacatgggag ataggttgaa tggttataat catacttttc ctagtgcttc tttcattgaa
    25081 agggaaagag gcattactag aagggatatt ttacatgaca aagaattatt agaacaattt
    25141 ggtccgcttg atcaaaatac aataaatgaa gaagaattgt taaatcaata taatataagg
    25201 ctagtgaatt gggaaatatc tgaaccgatt gttaacaatt ttggtgatta gaaaacaatt
    25261 acgataaata gaaacaagcc aaatcatcag caagtaaaag ctgatgattt ggcctttctt
    25321 atacgagtag taggaattca tagcgaatat tattacgatc agtagatatt ttgatattaa
    25381 aggagtatta cgaactagta tgtgagaatg tagaggtgat ttgcgaataa tgagaaattt
    25441 atcaaataga aacaaaatat taatcattat tgttgttata gctgtgtttc atctgggaac
    25501 gaatgcggtt ttaagtagaa taatattggg tcctaagcca ccaagacctg cgattacccg
    25561 aggggagttt gattttagat tagaatatga agtagatggg gaaaggattg tgatagaaga
    25621 tacaattgtt gcattatttg atggattttc tgcagatgca ggtagcatgg catggtatcg
    25681 cacgtggcgt ttgcatttgg cgagtgacaa gagggcacaa caattattgt tacatgagtt
    25741 agatgatggt cgaaaaatat attatcattt tggaggagct ggattttata tggggaagtt
    25801 agtagcagtg taactgtaac aaggaaagat ttgccaggtc ttggcattcg aaactccata
    25861 ttttatatcg agcgtgggag agatacatct attgggaaca ggaaagggtt aactcttgaa
    25921 gagttatata atgactttgg aatccgtctt gttagttggg aacacaatcc accaatagaa
    25981 aatcgctttg aataatcgaa atctattaat ccatttcatc agccagtgaa tgttgtaatg
    26041 gaacaatttg gtttagtgtt caaaaccatt gaaatactag gctgtgatca gcaaaaatgt
    26101 atctgaataa aaaagagagc aactattaaa tgagaggagt taggagttgt tagctttata
    26161 cgaatcaaag tggcaaatac agatgcgtat gcatttgtgt ttgccatctt gcgtttggta
    26221 gacgagatag gtataagcta aagaaacaac ttgtttaaaa taaacgacct agctcaacaa
    26281 acgaccatct aatcaactaa cagcatacat aaaagcaact atttttgaag tagaattgaa
    26341 atcgggataa cttaaaaagt gattgggata ctagtgtgaa tcaccgtaca taaatattac
    26401 tcgttaaggg aatttgagaa attttgtatg aaaatttaag aattagttag tatgcgcaag
    26461 tcaaaaatat gaataaatta tttggtggag ataatttgct cagcttgtca taagaaatga
    26521 gataatctgg cagatgcaag cttttggaca ccatttattc ggaataa
//
LOCUS       DBNIBO010000022        26389 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.022, whole genome shotgun sequence.
ACCESSION   DBNIBO010000022 DBNIBO010000000
VERSION     DBNIBO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.022"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(17..922)
                     /gene="xerC"
                     /locus_tag="AC1Y77_000814"
     CDS             complement(17..922)
                     /gene="xerC"
                     /locus_tag="AC1Y77_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIQ1574411.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1120..2496)
                     /gene="trmFO"
                     /locus_tag="AC1Y77_000815"
     CDS             complement(1120..2496)
                     /gene="trmFO"
                     /locus_tag="AC1Y77_000815"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HIQ1574412.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLDSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2465..4543)
                     /gene="topA"
                     /locus_tag="AC1Y77_000816"
     CDS             complement(2465..4543)
                     /gene="topA"
                     /locus_tag="AC1Y77_000816"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIQ1574413.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDIVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4665..5522)
                     /gene="dprA"
                     /locus_tag="AC1Y77_000817"
     CDS             complement(4665..5522)
                     /gene="dprA"
                     /locus_tag="AC1Y77_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIQ1574414.1"
                     /translation="MYQIEENLLKLAYAKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITTHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVVEAGYVIVSGCAKGIDTYGQQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHRFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5578..6345)
                     /locus_tag="AC1Y77_000818"
     CDS             complement(5578..6345)
                     /locus_tag="AC1Y77_000818"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIQ1574415.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6338..7198)
                     /gene="ylqF"
                     /locus_tag="AC1Y77_000819"
     CDS             complement(6338..7198)
                     /gene="ylqF"
                     /locus_tag="AC1Y77_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIQ1574416.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            7459..7857
                     /locus_tag="AC1Y77_000820"
     CDS             7459..7857
                     /locus_tag="AC1Y77_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1574417.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            8094..8636
                     /locus_tag="AC1Y77_000821"
     CDS             8094..8636
                     /locus_tag="AC1Y77_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIQ1574418.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(8719..9270)
                     /gene="lepB"
                     /locus_tag="AC1Y77_000822"
     CDS             complement(8719..9270)
                     /gene="lepB"
                     /locus_tag="AC1Y77_000822"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIQ1574419.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(9422..9649)
                     /locus_tag="AC1Y77_000823"
     CDS             complement(9422..9649)
                     /locus_tag="AC1Y77_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HIQ1574420.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(9646..10164)
                     /gene="msrA"
                     /locus_tag="AC1Y77_000824"
     CDS             complement(9646..10164)
                     /gene="msrA"
                     /locus_tag="AC1Y77_000824"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIQ1574421.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(10184..10897)
                     /locus_tag="AC1Y77_000825"
     CDS             complement(10184..10897)
                     /locus_tag="AC1Y77_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HIQ1574422.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(10900..11796)
                     /locus_tag="AC1Y77_000826"
     CDS             complement(10900..11796)
                     /locus_tag="AC1Y77_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIQ1574423.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(11954..12796)
                     /locus_tag="AC1Y77_000827"
     CDS             complement(11954..12796)
                     /locus_tag="AC1Y77_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1574424.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            12928..13581
                     /gene="trhA"
                     /locus_tag="AC1Y77_000828"
     CDS             12928..13581
                     /gene="trhA"
                     /locus_tag="AC1Y77_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIQ1574425.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(13645..14172)
                     /locus_tag="AC1Y77_000829"
     CDS             complement(13645..14172)
                     /locus_tag="AC1Y77_000829"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIQ1574426.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(14192..15139)
                     /locus_tag="AC1Y77_000830"
     CDS             complement(14192..15139)
                     /locus_tag="AC1Y77_000830"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIQ1574427.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(15151..17034)
                     /locus_tag="AC1Y77_000831"
     CDS             complement(15151..17034)
                     /locus_tag="AC1Y77_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1574428.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(17202..19274)
                     /locus_tag="AC1Y77_000832"
     CDS             complement(17202..19274)
                     /locus_tag="AC1Y77_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIQ1574429.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(19287..19562)
                     /locus_tag="AC1Y77_000833"
     CDS             complement(19287..19562)
                     /locus_tag="AC1Y77_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574430.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(19924..20379)
                     /locus_tag="AC1Y77_000834"
     CDS             complement(19924..20379)
                     /locus_tag="AC1Y77_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574431.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(20533..21741)
                     /locus_tag="AC1Y77_000835"
     CDS             complement(20533..21741)
                     /locus_tag="AC1Y77_000835"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIQ1574432.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(21853..22185)
                     /locus_tag="AC1Y77_000836"
     CDS             complement(21853..22185)
                     /locus_tag="AC1Y77_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIQ1574433.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            22333..23208
                     /locus_tag="AC1Y77_000837"
     CDS             22333..23208
                     /locus_tag="AC1Y77_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1574434.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(23290..23871)
                     /locus_tag="AC1Y77_000838"
     CDS             complement(23290..23871)
                     /locus_tag="AC1Y77_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HIQ1574435.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(23877..25136)
                     /locus_tag="AC1Y77_000839"
     CDS             complement(23877..25136)
                     /locus_tag="AC1Y77_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIQ1574436.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(25138..26184)
                     /locus_tag="AC1Y77_000840"
     CDS             complement(25138..26184)
                     /locus_tag="AC1Y77_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574437.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         7240 a         5468 c         4544 g         9137 t
ORIGIN      
        1 gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt
       61 cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt
      121 ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca
      181 tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt
      241 caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt
      301 cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt
      361 gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga
      421 cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc
      481 ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg
      541 ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat
      601 ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa
      661 gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc
      721 taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca
      781 gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat
      841 atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt
      901 gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca
      961 cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc
     1021 acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa
     1081 gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg ggattgcagt
     1141 tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg caattgcttc
     1201 atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac catagttggc
     1261 attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtatgcca tacttccaat
     1321 agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg cggcgttgat
     1381 tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag tcatctgacc
     1441 tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca attctggcga
     1501 attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat tttctaaccc
     1561 aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct gaaagcccac
     1621 gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag cataaggacg
     1681 tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa gcatcgtttt
     1741 gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt tttctttctc
     1801 aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag ctttgaattc
     1861 ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc gggacttcaa
     1921 atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg catcatagaa
     1981 ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat gagaagtcaa
     2041 aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt cgtttatcac
     2101 tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag aatcgcggtc
     2161 aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga ttactaccga
     2221 atccaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac tgtttcctct
     2281 taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct cagtcgattt
     2341 gactggacgc atttcatata aacggacagg gactcctgct tgtgcgattt gccaagcagc
     2401 ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa gaacctcttt
     2461 tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact tgtttaccgc
     2521 ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg cccactggtt
     2581 tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat agtctgtttt
     2641 tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc acaccgattt
     2701 cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca taaaacttcc
     2761 catatttccc taattttatt accatcggat gtccacagac atcacaatcg aatcctgctg
     2821 gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt tctttttcaa
     2881 acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct tcaattttat
     2941 ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg aagaattcac
     3001 agatcagtga attgacaatt tctcctagtt cagtcggctc aaatcttttt tgtgtcaatt
     3061 ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa ggtcgcccta
     3121 ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct ggtggttgag
     3181 taaaatgttg ttttggttca atatcaacag attggactat atcgccttct tcaagttctg
     3241 gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac acttgcatga
     3301 atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt tgttctaatg
     3361 tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa cgtgaccaga
     3421 tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca tctggtgtac
     3481 gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga gcatttttga
     3541 tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata aatgctgcgg
     3601 cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat gtaatcaagc
     3661 cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct accatcatgg
     3721 tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta cttgtagtga
     3781 aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact tcgaagtctg
     3841 ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat ttctttttct
     3901 taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta aaattaccgt
     3961 cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg tcaataataa
     4021 tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc ttacgccaga
     4081 gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct tgctgtgcat
     4141 ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact gcgtcttttg
     4201 tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt aggtacgcta
     4261 aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa actttttgag
     4321 ctttttttgc tgcggcttta aggcttttga tgacatcgcc ttttccgcgt atagaaatat
     4381 aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt aaatctctta
     4441 tatggccaac actggccatt actttatagt ttcgtccaag atatttctca attgtcttgg
     4501 cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa tggactcctt
     4561 tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct aatcatatcc
     4621 attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc tttaaattct
     4681 gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa atgatgacat
     4741 ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct tccaagatcc
     4801 aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa gacacatgtt
     4861 cctttagaca aagctgcgat aatccgattt ctcattggaa aacgatgctt tttcgggccg
     4921 gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc ttgcaagtca
     4981 cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat tgtccgtcca
     5041 ccatgagaga ttgctgcttg ttgaccgtat gtatctatcc ctttcgcgca gccactgacg
     5101 atcacatatc cagcttctac cactggtttt agtaaataat aaactgtttt tttggctaaa
     5161 gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa agatagatca
     5221 ccattataaa acagtacaat tggcggatag gtcaagtgaa gtagctgagg gggatatttg
     5281 ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt agcgtgaatt
     5341 tcctgccaac ttttgcgaaa cagctcccga tgtgtggtta ttttacctat cttaatgatg
     5401 tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag tgcccatttt
     5461 ccttgactac tgattccttt tgcataagca agtttcaata aattctcttc tatttgatac
     5521 atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg ctgtttttta
     5581 ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa ttccttgtcg
     5641 ttcaaggcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga aaccatatcc
     5701 aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag caatgatact
     5761 tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct gctggatagg
     5821 caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca atttttctac
     5881 tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga ttgtctgctg
     5941 atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat aaagaagttc
     6001 tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct catttttcgg
     6061 caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta cttcatcaat
     6121 gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa acatttcttc
     6181 atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag ccagtgcttg
     6241 ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat ctattctctg
     6301 tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt ttttgctcca
     6361 tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttggcgaata tcatgcacga
     6421 ctaattcgct gccgcgatca tagtcttctt tatacccacg ttttgctgta atatccatca
     6481 gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga tcgctgattc
     6541 ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca tctaaatgga
     6601 caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct tcatcttcaa
     6661 atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct aaacgaagcc
     6721 actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc ttgcctacta
     6781 gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt gcccgaatag
     6841 cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat gcttctttag
     6901 ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct aatgcaagat
     6961 ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct gccagatccg
     7021 ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc attggattac
     7081 gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca tatttcattt
     7141 tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg atggtcattg
     7201 ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt tttctttttc
     7261 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
     7321 cagtcctcat tctatcacat cttcactttt ttgatgatgt aaatttttat tttggtttct
     7381 atatatgaat ctaaattttt gacaacgttt cctaaataag agatgataga cgaacaaaac
     7441 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
     7501 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
     7561 gaagaagctg gttcaatggt atgggatgac actttttggg taatgcttct ctctcatgat
     7621 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
     7681 ttgatttcgt ttagtgttga aaacgcggat gaagtaaaaa aatttgctca aattgcaaaa
     7741 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
     7801 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
     7861 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
     7921 ctccgcctct atttttttca ttcgtacaat ctctttttta cagtttataa aagcagtctt
     7981 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
     8041 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
     8101 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
     8161 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
     8221 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
     8281 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
     8341 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
     8401 tatatcataa aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc
     8461 cagtcagcct ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag
     8521 tgatttttct aggtagagtc atcaggaata tgatccaaat ccctattcaa ataatggttg
     8581 tttattcatt tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt
     8641 acgtactaag gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagat
     8701 tgcacgcttt attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa
     8761 tcttgcttta cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga
     8821 aactcttcga ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt
     8881 tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt
     8941 tttagttaga taaggttcct taactacttt cccattgatc ttcaattgat cattttggta
     9001 gctgacattt tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa
     9061 ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc
     9121 accttgcttc aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa
     9181 tccccgcaac atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa
     9241 gcgattaata tagcgttgtt tttttgtcaa tctctatcaa tccatccctc gttttttcat
     9301 tctttcttta ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata
     9361 attctttcaa ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct
     9421 cttatatgtc atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg
     9481 acggcaaata gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt
     9541 gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg
     9601 tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct
     9661 ccaattttct tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg
     9721 ctttttataa tactcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat
     9781 ttttgtaacg attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc
     9841 agcaatgatc cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg
     9901 atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa
     9961 ttgttcatat gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt
    10021 cgtttggctt tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga
    10081 catcactgta tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaaacagcc
    10141 tcctgcaaat attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt
    10201 ttttattttc ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt
    10261 tgtttgaagc atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta
    10321 ctaagtcgat tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc
    10381 taagcatgat agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc
    10441 tcttgatcat attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc
    10501 tagcttttag ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat
    10561 cgataggaaa acctagtact tcgaaagtac cattgagtag tgcttcattt tccaaataaa
    10621 atttatattc aatatcacta tctttttgat actcactaag gaaaaagctg atgatttgat
    10681 tgactttttc tttatttgaa ttgatcgtta agacaggatc tccttgtctt tccgtaagag
    10741 ctggtatttc tgggagttct ggttcccgat tagcaaaaat acggctgcct aaaaatacgg
    10801 tcgaaccaag tactacccca agaaggataa ggaaggcgaa tttccagtaa tttgttttct
    10861 ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt tattgatcat cctccttctc
    10921 tagccattgg ttttgtgtct cgatcagctg atctctcact gcatttgcca tcaattgata
    10981 acctaaatta tttggatgga aatgatcttc ttcatagagc acattatttt tgatgccatt
    11041 tagattttct gtcgtgctag ttgtttgatc tgttccttct tctgtgacac ctacttcctg
    11101 attcaatcct tgataaagca aatcattgat tgggataaag tagcagttcg gcattttttt
    11161 tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt gtctgcatgt cagtgatctc
    11221 aggaaagttc aaataaaatg gattgtagat gcctaaaatg taaataggtg cggttttatt
    11281 gagggatcga aattcttcca atagttctgt gacattttcc tgatagtttt tcagtggctt
    11341 tttaaaggaa ttgaccgtta acccgaataa attattttgg ataactttca tcaaatcatt
    11401 accgccaaca gtcagtgtga taaaatcagc tgttttgata ttgtttcgaa tttcttcttt
    11461 tgtttttact cgctttaaga tctggtcact tcgttcacca tttacaccat agttttctgt
    11521 ttcaaccgcg gtcaaattat aacggtcttt tagatcattt gcgacaaggg gaacgaatcc
    11581 tccttgcttg gtttgatctc ctactccttc agtcagcgaa tctccgatag ctatgtagtg
    11641 aagtttttct tttttatttg gatcttctct cacagtagtt actggtttca ttagtgggcc
    11701 agctttcggt atagaagcag ctaaacctgc atagatgatg atcgcaatta ataatgggaa
    11761 caaaacgagg atcagatgtc gtttcgttaa tttcattttt tcacctcttg tattcgattg
    11821 gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt
    11881 attttcctaa ttttataaag aaaaagagaa taagctgcaa gcctttcagg ctgagggctt
    11941 atcctctttt cgactaatct acataataca tgattgcaaa cgcgtcttta ccagtatgcg
    12001 tagcgataac cggattcgta tgcagtacag ggatatgcat gtccgggaat aattcttgca
    12061 aaccctcttt gaattcttgc gccaattctt ttgcatccgc atgtgaaata ccgatttgtc
    12121 gcacatttgg caattctttc agttcatttt tcaaattatt aaaccattta tcgaacgttt
    12181 tctttcctcg ccccttagtt acagggatta attcagaatg ggcgaaattc atcaccacgc
    12241 gtatgttcaa aacactagac agcaaaccag ttgttcgact gattcttcca ccttttacca
    12301 agttatctag tgtggatatt ccgatgtata attttgtatt gtcttgtact tttttgactt
    12361 cttcaaggat ttcctctact gttttacctt cttttgctaa ttcagctgct cgaattacct
    12421 gaaaagataa tccttggtca gtggtatcac tgtcgataac cgttactttc gtttttgtta
    12481 gattactggc ttgtcttgct gcttctactg ttccactcaa gccttttgtc atatggatcg
    12541 aaatcacttc actgccgtct tctcctagct catcatacat cgagataaat tctccaatcg
    12601 gtggttggct tgttttgggc agattttttg cttttgccat catttccata aatctttcgc
    12661 cgtctagttg gtcatcatcg ggatatactg ttccgtcgat catgatggat agcggcatga
    12721 cacgaatgcc taaagcatcg cgtacatttt tttccatcgt acatgaagaa tctgttacga
    12781 tttttacatt tgtcattatt ttctctctct ctttctttaa caagatgcct tattcatgtt
    12841 aaactacatg taagacaatt tctattttta gtataacaga caaataatga tttttcatta
    12901 attacaccta gtaaaggagc cgttttcatg gaaaaaacga aatttaatcg aacgtatgaa
    12961 atattaaatg aagtgtttaa tgcagtcacc catggaattg ggactggatt gagtatcgcg
    13021 ggactagtgg ttcttttagt aaaaggtgcc cacttgcatt cctccctcca tattgtctct
    13081 tatgctatct atggatcgat gatgattctt ttatttttaa cgtctacttt gtttcattca
    13141 ttgattttta caaaagcaaa aaaggtgttc caagtattcg atcatagttc gatttttctt
    13201 ttgattgcag gaagttatac tcctttttgt ttgctgagta tccgaggatg gctaggttgg
    13261 actttatttg tattaatctg gttgtttgcg attcttggca ttatatataa agcgttgacg
    13321 ttgcataaac gggagagagt atcaaaagta tctacgatca tctatatttt tatggggtgg
    13381 ctctgtgtcg tggctgctgt tcctctttat cactctcttg gaatgactgg tgttattttg
    13441 atggtagcag gcgggctttc ttacacgata ggcgcattct tttatagctt gaattctatc
    13501 cgctatatgc atgttgtttg gcatttgttt gttatgttag gtgcaggttt tatgtacttc
    13561 tctgttttat tctttactta ataaaaaaaa ataaagctat ggatgggaca gctctaaata
    13621 acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc tttttacagg
    13681 attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt catctatttc
    13741 tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa aataagcatc
    13801 tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg gcagaaattc
    13861 ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt tttccagagc
    13921 aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg aagaatctct
    13981 cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt cgaaagtttt
    14041 tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca gatcatttgg
    14101 caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct gtgcccaaat
    14161 tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct ttgatcgccg
    14221 gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat actgactgct
    14281 tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat tgttctttca
    14341 tttgttcgat atgattgata taaagatgag cgtctcctaa tgtgtgaaca aattcgccaa
    14401 ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt gcaatgttga
    14461 acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt agccgtccct
    14521 cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta ggaacatctt
    14581 ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg attgcttcga
    14641 tcacttcttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga cgccattgat
    14701 agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct
    14761 tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga tctgtcaaac
    14821 atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg atgtagcgtt
    14881 caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg atattcgtat
    14941 ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg
    15001 ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat aagcttaatg
    15061 tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt cttcctaagt
    15121 caagatacgc ttgttccatc ctttttcctc ctaatcttct acccattcac tcaagtattc
    15181 ccaccgtgcc attttttctt caagttgtgc ttctgtttca gatacgtcgt tttgcaattc
    15241 ttgtaatcga gtgaaattat ctccttgatg attcatctct tcttggagca atgagatttt
    15301 ttcttcaagt tctgcaatct catcttcaat cgtttcccat tctttttgtt ccatgtaaga
    15361 taatttcttc ttttcagcag gttccttgct ctgtattttt ggtttcgctt tttcagcagg
    15421 ctgctgggtc tctttttgct tcagtaaata actggacatg tttccgtaat aaagttcttg
    15481 ttttccgttt ccttggaata ctaataactt atctgctacc ttgtccaaga aataacggtc
    15541 atgggaaaca gtgatcacag cacctggaaa acttctaaaa taatcttcca aaatcgtcaa
    15601 tgttgctatg tccaaatcat tggtcggttc atccaataat aaaacattag gttgctggat
    15661 cagcagtttc aacaaaaata accggcgttt ttctccgcct gaaagtttgc ctatcagtgt
    15721 cccatgcata aatcttggaa ataaaaatcg ttcaagtaat tctgctactc cgatttgact
    15781 gccatctgct gtttttgctt cttcagcagc ttcttgtaag taagcaatca tccgtttatc
    15841 tggtgccatt tcttcatttt cttgtgtata atacgcaaga tgaaccgttt caccgataga
    15901 cattgtaccg ctatccaggg gaattctgcc agctagaata ttaagcaatg tcgattttcc
    15961 cgcaccattt ttaccagtaa tacctagtct ttcccgagat tgaatcagta aatctaaatg
    16021 ttctagcagt gtttggtcat ttatacggta actgccatct ttgatttcca gtactttttt
    16081 ccctaaacgt tgtgtcgcta ggttcagctc taaatcatct tcttggttta cttggtacaa
    16141 gttctccttt agatcttcga aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg
    16201 tacgccagcc ctcatccaag ctaattcctg tttataaagc cgcttgcgct tttcttcttg
    16261 ttctacttct acacgatcac gttctgcttt ttctaagacg taagcttcat aatttccttt
    16321 atactcgtat aattttccaa atgaaagttc gaagattcgg tttgctactc tatctagaaa
    16381 ataacgatcg tgggtaacca tcaatagtgc gccacggtat tgtttcaaat agttttccaa
    16441 ccactcgatc gcttcataat ctaaatggtt tgttggttcg tctagtaata gtaaatcggg
    16501 ttcatcaatc aacacttgcg ccagactaac tcgttttttc tgaccacctg ataaagtact
    16561 gattttttta tctaattcac tgattcctaa tttttgcaaa ataatctttg cgttggtatc
    16621 tgtagtccaa gcatcttctt tattcatttg ttcttctgct ttagcataac gtttttgcac
    16681 atcagggtct tctccgtttt cccctagttc gatcaatgct tcctcatatg ctcgaacagt
    16741 ttggatcaag gggctgtttc cttgaaagac tgcttggaga acagttaatt catcggaaaa
    16801 ttcagctgct tgagaaagat aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg
    16861 atcaccgtcg ccgctatcga taccggcaat aatatttaag agacttgttt tacccgtccc
    16921 gttcgtccca atcaacccga tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa
    16981 caaatctttt tcaccataag tttttttgag gtcagttact tttaattctt tcaacatgtt
    17041 ccctccttat cttggatatt gtatcataaa aatagtgaac ttcaggaaaa tttttttggt
    17101 atttgttctt tttgtgttct caaaaaaaga acaggaccga ttcgatcctg ttctgatatg
    17161 ttttgttttt tataaaactt gaaaattttt acctttttat tttattggac tcgttttttt
    17221 gactttgctc gaaacgcaaa tcggaaagaa aagttatcag atggtttgtt catcgtataa
    17281 gattctaaca aattgatatt cctgcgatag ctgttcgcag aaaacgtcgt gattttctca
    17341 gcaacttcgt attcatcaat gatttttctt tcaagataat aacctcttag catttttttt
    17401 gtaaacagac tatcttgctg gatcatccac gaagaaatca aattccccga gttcattttt
    17461 attccttcaa gtttgcgttg ctggataaaa aattgaacca tcgtttcatc ataaatatca
    17521 gtcaagttat ctaaactttc aataatcaac tgagtatttt tttgatatgt ctctctgatt
    17581 tcttggatat cttcatcact aataaatgtc tgacgatcag catgtcttct ttgccaaaac
    17641 attttcggat ggagaacgat tcgcattaac ctacggacaa caatgaacca aaagccgata
    17701 aaagacaaaa tctggcttgt gatagaatgt tcgaatcgag aaagaaaacg ggagtaaaaa
    17761 cgataaccat tagaagaaag cttgcccgaa cgataacttt catccaatcc atcccgttcg
    17821 atcgacaaaa tcagtgcttg tatttcttgt acttcttgct tttcatcatc ggttaaatct
    17881 tccaagtaca gatcacgtat ccgttcttga taattttcta tcactgcttc agtggctaaa
    17941 aattcttttg tattacgatc aatttccttt atttcttttc gcaaaacttc aacgacctct
    18001 tctaaaatca tcaaagcatt caaatccgtg cttttcgctt cctctgtttc cgtcagcata
    18061 ggcaagacaa tgatccctat gaccaaagtc actaagatga cacccgccgt cagaaacaaa
    18121 agcaaagacc gctggtaaaa catcatatta ttgattgaaa atggcaaaat aaaaatcgta
    18181 gccaaactga ctgttccctt tacaccgcca aatgttaaga tcaagatctc gttcaactgt
    18241 tttttgaact ttttactgcc atctttgaat acataaaaaa gactgataga aatgaatctg
    18301 atcacaaaaa gcatgacaga gattaatacg ataatcgcta ataaaagacc gtttggatat
    18361 aaaccgtctc cccatactgg cgagaacact tgcgtcaatt cgattcctaa aaatataaat
    18421 actaatgcat tcagtgtaaa agcaatagtt gtccatgtac tgtgggacaa gctcgacaat
    18481 tctgcatcaa acacagagat tttcctaaaa ccagaagctt ggagaatacc tgctgccact
    18541 gcagcaatga ttccagatac atcaaatgcc tctgccaata cataggctag aaaaggaagc
    18601 aaaagttcga tcagcagata ccctgtgaca tcttgtgctg atgctttttc gatcaaatga
    18661 attatttttt gtttgaacca gacaagcaaa aagccgatca aagctccccc aatactagaa
    18721 actactagac tcatccccgc gtttactgct gaaaaagatc cggtgatcag cgcaccaaga
    18781 gcaaactgga aggcagtaac accagatgca tcattcaaaa gaccttctcc ttccaaaata
    18841 tgcatcgcct ttttagggat attcactcgt ccagaaagag aactgactgc taccgcatct
    18901 gtcggaccta acgctgctcc aaaagcaaag caggcagcaa gtgggacact tggcaacagg
    18961 aatgataacg tcgcacctac cccgacaagg gtcaaaatga ctcctccaaa tgccaagaac
    19021 aaaattgtcc cgaaattttt gagaatcgaa gaaatatctg ctttttctcc ttctctaaat
    19081 aacaatggtg caataatcat aactaagaac aattcaggtt caaaatcgat tgattgtccc
    19141 cactctgtca gacctaatac cactccaagg aaaatttgga tcagtggagc tggaatcatt
    19201 ggcaaaatgc ggctagctat gttcgataag gtgatcgtta ctgcaaatac aataactaac
    19261 tcagcaaatt ccatcgtcca tcccctttat tttttatttt gtgtaagcat ttttaaaaat
    19321 tgttcttttt tctttaaaat tttggtttcg actacttcaa cctgtgtttg gttaccctct
    19381 aattcacact gtacacgttt tgactccaaa cgaagcagat catgtttcag acgttccaac
    19441 tgacgataca tcaatatatt tcttttacgc aacttacaaa gtatttgacg gcggtcatcc
    19501 atcgaaaggg cgccatagcg atcatgcaac agtttttttt gttgttcaaa actctctctc
    19561 attttccaga tctcctatac acaaacttcc atcattatag caaagttctt tttggatgaa
    19621 aaatatttca ttgtttcatc gtttattgct agttagaaat ttccataaat ttttcaacgc
    19681 ctcattctat tccaaatagt atcagatatc aagtaaaact taagaaagat aaaagtttct
    19741 gacaaaagaa tgtttacttt tccattcctg cctttcttgt tcttttatca caacaaaaaa
    19801 atggggctgc gacaaaaatc ttgtcacagt cccttattct gaataaacgg tgtggaagag
    19861 acagcctatc agtagtaata agacacatct ctttcacagc ttcatttttg cgtaaaaatc
    19921 gatttattcg aaaaattccc cgtatacatc ttctacacgt tgatctggat tgatcacgat
    19981 atacaagtta cctgtttttt cgctgatttt tgttgattga tattgattgt ctaatccttc
    20041 tgttccttta tctagatatg ggaagtacac aagatcaggt gtgccatttc ggtaaaggat
    20101 ttccattcgc tcgatatctt cgtatttcaa tgcgcgatta aacatgccca tttccagtcc
    20161 gcctaggttg atatcatgtg tcgcaactgc gtccccttca tggaagattt cgattttaaa
    20221 tccagcacac ggatggattt caacaaattc actgccattg attcgtccaa aacttgttgt
    20281 gatttgtttg atccaaagat cgccgatatg acggcggttg atcgtccagg tttctccatt
    20341 tctaaaataa aattttagcg ctgtcatttc tttactcatt aaaaagactc ctttatgatg
    20401 taatttattc ttgtactacc tcagtttagc acaaatttat gtaagtggtc acatcttatg
    20461 ttttttcttc taatgacgta tctgcccact ttaagtagcc aacaaagctg agcagataca
    20521 gtctagtgtt ttttaaatca atttttcttt cgataaaaaa tccaaaacct gttttttatc
    20581 attttttatt ctttgttcta cgacggcttt ttcagctaat tgcaaagctt cactaaccca
    20641 cttaccaggt ttttttccag tttcttctag caagatttta ccatctactg ctaattcgct
    20701 taatgacttg ataggtaaac gatcatagga ttctcctacg gcatccatct gaggtgtttc
    20761 gtcaaaatag atcatcaatt cttctacttg cagtgcatgt tttttgccta agaagtacaa
    20821 ttcgaatggt tccaaagctc cagtttctcg ttgtcgtagt ccttggacaa gtgcttgaac
    20881 gtctttaatc atttggttag aacacttcca cgatttcaaa aaggaacgaa tctcattttc
    20941 ttttagaccc agttggctga tcaataatgt ccatacttgg ctctcttctt tcaatgggac
    21001 ggatggtaat tcagcaaaac gtaaaagtgc ttcgccttct tctttcaaac tgggacagca
    21061 aatgtagcac tctgtttcaa taaatgcatg gattcccgcg gcccgttcat ttcccaagag
    21121 catcttgatg aattccacat ttatacgttc tacagatatt ttacctaata gctcgtggtt
    21181 ttctttgatg gcgtagaagg tttcttcctc taatttgaac cctagttgac tgacaaaacg
    21241 caacccgcgc atcattcgta aggcatcttc atggaaccgc tcatgaggat tgcctacagc
    21301 tcgcaagatc tgttcttcta aatcttgcag accatcgaac aaatcgatga tttctccatc
    21361 ttctttcaaa gcaaacgcat tgatcgtaaa atctcttctt ttcaaatctt ctgccaacga
    21421 acgaacgaat tcgacatgat ctggtctgcg gtagtcctga taagttgatt ccgtccgaaa
    21481 tgtggtgatc tcatattgtt catcattttc taataccaac acagttccat gttcgatccc
    21541 aatatctatc gtttttggaa aaagctgttt gatttcttct ggaaatgcgc ttgttgcgat
    21601 atccacatca tggattggct ggttcagcaa gatatcacgt acacttccac ctacaaaata
    21661 agcttcatat cctgcattct ctaatttttt cagaactgga attgcctttt ggtattccag
    21721 aggtaattgt tttaatttca tttttccaac ctttcctgcg tgtttcttgt actgagataa
    21781 agactcagtt gcatggtgac tgtttgacaa gtccgtttca gagtcttcaa ggccaggacc
    21841 acttcatctt gtttactcgt ccttacgctc aaagcgatta tggtctcttt gattgaattt
    21901 ttccatgttt ttttcaaaaa cttcagttaa gtcgatattc aatgaattag ccatgatcat
    21961 cgtaacgaat aaaacatcac caagttcttc tgctaccgag ttaactggtt cagaagcctt
    22021 tttgcttttt tctccataag tgtggttgac ttctcttgcc aattcaccta cttcttcagt
    22081 taatcgagcc atctgagcta atggagagaa ataccccacc ttgaattgct ggatatattc
    22141 atccacttcc tgctgcatac tgtataaaga acgcttttta tccatctttt tccctccaaa
    22201 actatcttac caaaacttta acgcaaatcc tagataagag cagttgagaa aatcgaattt
    22261 ccatgctaca tttagaaagg aatttctaaa tggaaattct gtgaaaatac ataattgaaa
    22321 gctggaattc atatggtcaa acgaaaaaca gtaaaagaag ttctttttat cattatagga
    22381 acaagtattt atgcatttgg tcttgtttac ttgaacatcg ctaatcattt agcagaaggg
    22441 ggcgtctcag ggatcacctt gatcttaaga gctttattcg gaattgatcc agcttatacg
    22501 actttattaa tcaacatccc attgatttta ctaggaggaa agatcctcgg taaacgttcc
    22561 ttggcctata cagttctagg tacactttct ttgtcattct ttttatggat ctggcaaagg
    22621 gttccactag aaatcaattt acagcatgat ctactgatcg tttctctact tgcgggttta
    22681 gttgcaggtg taggaagcgg gatcgtctac cgcatgggtg gaacaacagg tggcagcgat
    22741 attattgctc ggattttaga acaaaattac ggcatcacta tggggcgttc cctattggca
    22801 tttgatatca tcgtcttact tgcttcatta acctatatcg acttgaaacg tatgatgtat
    22861 acgttgattg tttcgtatgt tttcagtcgt gtgattgatt cgatcttaga tggcggatat
    22921 tctgcaaaag gtctacttgt cgtctccaac aaaagcgaag agattgcacc actactgatg
    22981 accggtctgc aaagaggtgt gacttttctt tctggagaag gcgcattctc tggtaattca
    23041 aaaaaaatca tctatatggt cgttagctct agtgaattaa ctgaagctaa gaggatcgtc
    23101 cacgagattg acgaaaaagc tttcctttca atcatcaatg tgcatgaagt ggaagggcaa
    23161 ggctttactt atttgaagcc acagacaaga ttactgaaaa aactataaga aaaaagacaa
    23221 agataggcta tgaggaaaga ttccacatcc agctgatctt tgtctttttt agtactatac
    23281 tatttttaac taattgttat gttcacttac ccggttactt ttcttttgtg tctgcaaaag
    23341 gatctttcgg ttaacttcat cagataattt caaaaaagca tccctatctc catcttctag
    23401 tgcttgatcg atttgagcat aaagcatttt tagttttgcc tcttcttctt tttcttgaaa
    23461 atatcgttca attaattcag tatcttcttc actgactgaa gaatcccaag aagaaaatgg
    23521 attgtcttct aagatagata gatattctct tgtctgccaa gcattttcga atatacattc
    23581 cacatataaa ggctttttcc aattcaaacg gatttcatga aaaatctggt ctgtgtccgt
    23641 aaattctttt ccctgcaaaa acaatttcat gggttcccct tcaacagata gatcactgat
    23701 ctgcagtcct cgatccgcaa aattcgcccc ttcaacaaag tggacattgc ttaaaatcgc
    23761 ttcatgattg gcaagataat tcaagatcca aaaaacttct cttcggctaa aagatacatg
    23821 attgaccatc cacgttaaaa aattcttctt ttcccgtaca tcaattagca tttttctcac
    23881 ctctctgtta ggtcatctaa taatgacagt gcctccatat ctcctggttc aagtgttaaa
    23941 taataagtca ataattcttg cgctcttttc agctgccctt cttctcttaa gaaaaaggca
    24001 tactctttca agaaatctgg ttcatgtgat agttcatgat aagcctgttc atagtgtaca
    24061 gctgcaacgg taaagtcttc taattcatta taagcatggg ctaggttcca ttctgcataa
    24121 ggattagcag tttcttccat ctgattgatc acttgtatca catcttcata tctttcatca
    24181 tccaagtaaa gattgcttaa agtaagtaat gtttcatctt gtttgtctcc tacttctaaa
    24241 gctttcagta aaagttcttc tgctcgctgt ctatcatgca gacggaaagc attttccgag
    24301 gccaggtgat aaagttcgac ttggtaagga ttttctttga ttccttcttc taacactgtt
    24361 tgtgcttctt caatcatttc ttcttcttga agagcttgtc ctagatatag atataacgat
    24421 tgataatgcg gattaacttc tcgaagttct tccagatagt tgattgcctg ctgattttct
    24481 ttcaattgaa gattcgtaaa agctagctgg aacagtcgat catctgtcct ttcttctttc
    24541 agtgcttctt ctaaaggtgc gatagctgct tcgaattctc cttgcatact taaggattgt
    24601 cctaaacgtt cctgcatcga gattcctgaa atttcattta tcccgcttgc caacaatgtt
    24661 tcataaacca ctgctgcctc tttgaaacga tcaactgaaa aatacaattc agctagcgca
    24721 aactggatca atggttcatc aggaagcaac ccagctgctt ctttcaattt tgcttcactc
    24781 acttcaggga ttccgataac ttggtacaaa tcagctatcg ccaataagct ttgcggatag
    24841 taatcacttt gcttatccac tttttccagg tattcaaaag ctaagtcgat ctcattatct
    24901 tcgattgcaa tttctgctaa cgggatgttc agtccgtcat tttctgggta ttggttgatc
    24961 agttgttcaa aaatctgttt tgcttcgatc aaaaagccaa tagccagcaa ttcttcgcct
    25021 agttcagcta atatatctgc tgggtcttct tttaatgctt tttctaaaaa gatttgtgct
    25081 tgtgacaagt cttcatctga taaagcttgc aacatttttt cgctatttgt attcatttca
    25141 attccctctt atctcactga cgatttgttg aaataccgtc attgttctca ataattcttt
    25201 ttcgtttgga cgttggtatc cgtctattat acctattttt tctaaaacaa gtattttttt
    25261 gcttttttct gccaaaaaaa gaactttttc caaatactcc atctttgaaa tctgttccag
    25321 ccagtggatt ggatagccaa gcaagtccag ccacacaaaa aagtttttta gttggaaatc
    25381 aaaaggatat gcttggagat tccacacgaa atgaaacatc attcctaaaa attttttcat
    25441 actagcagaa agtagatggc cattttcggt cagataaaaa gcttgttcaa acagtttacc
    25501 ataatttgac aggttttctg cctctaattg ataaaaatag atcatttctt cgatcatccc
    25561 ggcaaacggc cgagtcgtta attgcttttt atttggatag tttttataca gattttgcaa
    25621 aaaacgatga tcacaaatca ttccgcaaca gataaaagtc aaaaagtcaa tctgctttcc
    25681 gtccacttgc cgctcgctga ttgtttgatc ataaacgatg cgttctggca gattgatggt
    25741 ttgcagtact acacgatttg gtagctgcaa aatcgtccgc tgcggtgtta atgctcggct
    25801 tagagaacgg atcgatacag ggatcatcca tagtgatgaa tcaagggttg tatgtttttg
    25861 aaaaaaacct gccagatcca tgatcccttc atttccaaat ccgatgataa acatggattg
    25921 attttcggga tagcgtttga cgaaatcaag caggttcttt aattctgtta aatgattgca
    25981 ttgagtgttg gcacaaatat accagtcatt gtttgcttga tttgcaaata acgggttgat
    26041 cttatctgca aacaaatcat aatagcgctg attcgttaaa aataagattg gctgatcaat
    26101 ggaaagttct cttagctggc tgactagcgt ttctccgtaa aacactttcg tcaacgcatc
    26161 ttctacttga taatcaagtt ccatacgcat ccctcctctt cttattttta ttttaccatt
    26221 agtaagcaaa ttctagaaaa gataagagca aagctcacct cataacttgg aaataaataa
    26281 aaattaaata aaatacatgt gttttctatg tatgacatac ggattcggaa tctctgtctc
    26341 ttatttattc cttttctgct aggaaataaa aagaaaagca cttgtagcc
//
LOCUS       DBNIBO010000023        26118 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.023, whole genome shotgun sequence.
ACCESSION   DBNIBO010000023 DBNIBO010000000
VERSION     DBNIBO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.023"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(82..777)
                     /locus_tag="AC1Y77_000841"
     CDS             complement(82..777)
                     /locus_tag="AC1Y77_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HIQ1574438.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="AC1Y77_000842"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="AC1Y77_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIQ1574439.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="AC1Y77_000843"
     CDS             complement(1976..2167)
                     /locus_tag="AC1Y77_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIQ1574440.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="AC1Y77_000844"
     CDS             complement(2188..3078)
                     /locus_tag="AC1Y77_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIQ1574441.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="AC1Y77_000845"
     CDS             complement(3065..3832)
                     /locus_tag="AC1Y77_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIQ1574442.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="AC1Y77_000846"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="AC1Y77_000846"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIQ1574443.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="AC1Y77_000847"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="AC1Y77_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIQ1574444.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="AC1Y77_000848"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="AC1Y77_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIQ1574445.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8403..8786
                     /locus_tag="AC1Y77_000849"
     CDS             8403..8786
                     /locus_tag="AC1Y77_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574446.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8954..9712)
                     /gene="jag"
                     /locus_tag="AC1Y77_000850"
     CDS             complement(8954..9712)
                     /gene="jag"
                     /locus_tag="AC1Y77_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIQ1574447.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="AC1Y77_000851"
     CDS             complement(9730..10548)
                     /locus_tag="AC1Y77_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIQ1574448.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="AC1Y77_000852"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="AC1Y77_000852"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIQ1574449.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="AC1Y77_000853"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="AC1Y77_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIQ1574450.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="AC1Y77_000854"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="AC1Y77_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIQ1574451.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="AC1Y77_000855"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="AC1Y77_000855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIQ1574452.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14567..14809
                     /gene="yaaA"
                     /locus_tag="AC1Y77_000856"
     CDS             14567..14809
                     /gene="yaaA"
                     /locus_tag="AC1Y77_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HIQ1574453.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14796..15920
                     /gene="recF"
                     /locus_tag="AC1Y77_000857"
     CDS             14796..15920
                     /gene="recF"
                     /locus_tag="AC1Y77_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIQ1574454.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15917..17863
                     /gene="gyrB"
                     /locus_tag="AC1Y77_000858"
     CDS             15917..17863
                     /gene="gyrB"
                     /locus_tag="AC1Y77_000858"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIQ1574455.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17883..20354
                     /gene="gyrA"
                     /locus_tag="AC1Y77_000859"
     CDS             17883..20354
                     /gene="gyrA"
                     /locus_tag="AC1Y77_000859"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIQ1574456.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20590..20889
                     /gene="rpsF"
                     /locus_tag="AC1Y77_000860"
     CDS             20590..20889
                     /gene="rpsF"
                     /locus_tag="AC1Y77_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIQ1574457.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20939..21451
                     /gene="ssb"
                     /locus_tag="AC1Y77_000861"
     CDS             20939..21451
                     /gene="ssb"
                     /locus_tag="AC1Y77_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIQ1574458.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21477..21713
                     /gene="rpsR"
                     /locus_tag="AC1Y77_000862"
     CDS             21477..21713
                     /gene="rpsR"
                     /locus_tag="AC1Y77_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIQ1574459.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21856..23829
                     /locus_tag="AC1Y77_000863"
     CDS             21856..23829
                     /locus_tag="AC1Y77_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIQ1574460.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23835..24287
                     /gene="rplI"
                     /locus_tag="AC1Y77_000864"
     CDS             23835..24287
                     /gene="rplI"
                     /locus_tag="AC1Y77_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIQ1574461.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24561..25928
                     /gene="dnaB"
                     /locus_tag="AC1Y77_000865"
     CDS             24561..25928
                     /gene="dnaB"
                     /locus_tag="AC1Y77_000865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIQ1574462.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7924 a         5143 c         5182 g         7869 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtact taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14461 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14521 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14581 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14641 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14701 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14761 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14821 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14881 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    14941 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15001 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15061 aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg
    15121 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15181 agaccaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15241 atcttgtcca ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg
    15301 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15361 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15421 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15481 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15541 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15601 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15661 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg
    15721 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc
    15781 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15841 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15901 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    15961 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16021 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16081 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16141 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16201 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16261 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16321 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16381 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16441 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16501 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16561 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16621 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16681 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16741 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16801 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16861 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16921 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    16981 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17041 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17101 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17161 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17221 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17281 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17341 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17401 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17461 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17521 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17581 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17641 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17701 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17761 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17821 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17881 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    17941 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18001 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18061 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18121 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18181 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18241 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18301 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18361 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18421 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18481 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18541 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18601 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18661 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18721 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18781 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18841 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18901 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    18961 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19021 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19081 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19141 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19201 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19261 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19321 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19381 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19441 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19501 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19561 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19621 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19681 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19741 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19801 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19861 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19921 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    19981 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20041 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20101 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20161 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20221 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20281 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20341 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20401 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20461 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20521 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20581 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20641 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20701 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20761 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20821 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20881 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    20941 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21001 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21061 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21121 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21181 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21241 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21301 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21361 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21421 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21481 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21541 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21601 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21661 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21721 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21781 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21841 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21901 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    21961 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22021 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22081 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22141 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22201 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22261 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22321 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22381 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22441 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22501 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22561 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22621 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22681 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22741 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22801 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22861 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22921 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    22981 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23041 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23101 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23161 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23221 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23281 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23341 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23401 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23461 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23521 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23581 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23641 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23701 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23761 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23821 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23881 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    23941 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24001 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24061 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24121 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24181 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24241 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24301 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24361 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24421 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24481 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24541 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24601 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24661 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24721 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24781 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24841 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24901 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    24961 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25021 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25081 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25141 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25201 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25261 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25321 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25381 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25441 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25501 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25561 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25621 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25681 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25741 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25801 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25861 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25921 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    25981 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26041 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26101 gtattagctc agcaaacg
//
LOCUS       DBNIBO010000024        25530 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.024, whole genome shotgun sequence.
ACCESSION   DBNIBO010000024 DBNIBO010000000
VERSION     DBNIBO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.024"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(159..1469)
                     /locus_tag="AC1Y77_000866"
     CDS             complement(159..1469)
                     /locus_tag="AC1Y77_000866"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIQ1574463.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="AC1Y77_000867"
     CDS             complement(1481..2899)
                     /locus_tag="AC1Y77_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1574464.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="AC1Y77_000868"
     CDS             complement(3139..4707)
                     /locus_tag="AC1Y77_000868"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIQ1574465.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="AC1Y77_000869"
     CDS             complement(4711..5121)
                     /locus_tag="AC1Y77_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HIQ1574466.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="AC1Y77_000870"
     CDS             complement(5118..6041)
                     /locus_tag="AC1Y77_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HIQ1574467.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="AC1Y77_000871"
     CDS             complement(6038..6916)
                     /locus_tag="AC1Y77_000871"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIQ1574468.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="AC1Y77_000872"
     CDS             complement(6903..8240)
                     /locus_tag="AC1Y77_000872"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIQ1574469.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="AC1Y77_000873"
     CDS             complement(8243..9346)
                     /locus_tag="AC1Y77_000873"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIQ1574470.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="AC1Y77_000874"
     CDS             complement(9420..10928)
                     /locus_tag="AC1Y77_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIQ1574471.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="AC1Y77_000875"
     CDS             complement(10894..11586)
                     /locus_tag="AC1Y77_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIQ1574472.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="AC1Y77_000876"
     CDS             complement(11608..12582)
                     /locus_tag="AC1Y77_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574473.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="AC1Y77_000877"
     CDS             12882..13754
                     /locus_tag="AC1Y77_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1574474.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="AC1Y77_000878"
     CDS             complement(14212..16410)
                     /locus_tag="AC1Y77_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574475.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="AC1Y77_000879"
     CDS             16642..17634
                     /locus_tag="AC1Y77_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574476.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="AC1Y77_000880"
     CDS             17694..19115
                     /locus_tag="AC1Y77_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HIQ1574477.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="AC1Y77_000881"
     CDS             19108..20607
                     /locus_tag="AC1Y77_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIQ1574478.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="AC1Y77_000882"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="AC1Y77_000882"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIQ1574479.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="AC1Y77_000883"
     CDS             22199..22882
                     /locus_tag="AC1Y77_000883"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HIQ1574480.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="AC1Y77_000884"
     CDS             22884..23720
                     /locus_tag="AC1Y77_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIQ1574481.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="AC1Y77_000885"
     CDS             23731..25485
                     /locus_tag="AC1Y77_000885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574482.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7787 a         5195 c         4480 g         8068 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt
//
LOCUS       DBNIBO010000025        25317 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.025, whole genome shotgun sequence.
ACCESSION   DBNIBO010000025 DBNIBO010000000
VERSION     DBNIBO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.025"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..611)
                     /locus_tag="AC1Y77_000886"
     CDS             complement(<1..611)
                     /locus_tag="AC1Y77_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIQ1574483.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQE"
     gene            complement(627..674)
                     /locus_tag="AC1Y77_000887"
                     /pseudo
     CDS             complement(627..674)
                     /locus_tag="AC1Y77_000887"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(988..1902)
                     /locus_tag="AC1Y77_000888"
     CDS             complement(988..1902)
                     /locus_tag="AC1Y77_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1574484.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1917..2918)
                     /locus_tag="AC1Y77_000889"
     CDS             complement(1917..2918)
                     /locus_tag="AC1Y77_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIQ1574485.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(2915..5098)
                     /locus_tag="AC1Y77_000890"
     CDS             complement(2915..5098)
                     /locus_tag="AC1Y77_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HIQ1574486.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(5101..7548)
                     /locus_tag="AC1Y77_000891"
     CDS             complement(5101..7548)
                     /locus_tag="AC1Y77_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1574487.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(7532..7924)
                     /locus_tag="AC1Y77_000892"
     CDS             complement(7532..7924)
                     /locus_tag="AC1Y77_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1574488.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(8011..8508)
                     /locus_tag="AC1Y77_000893"
     CDS             complement(8011..8508)
                     /locus_tag="AC1Y77_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HIQ1574489.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(8625..8849)
                     /locus_tag="AC1Y77_000894"
     CDS             complement(8625..8849)
                     /locus_tag="AC1Y77_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574490.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8953..10863)
                     /gene="ltrA"
                     /locus_tag="AC1Y77_000895"
     CDS             complement(8953..10863)
                     /gene="ltrA"
                     /locus_tag="AC1Y77_000895"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIQ1574491.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11647..12852)
                     /gene="mobT"
                     /locus_tag="AC1Y77_000896"
     CDS             complement(11647..12852)
                     /gene="mobT"
                     /locus_tag="AC1Y77_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HIQ1574492.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(13031..14416)
                     /locus_tag="AC1Y77_000897"
     CDS             complement(13031..14416)
                     /locus_tag="AC1Y77_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIQ1574493.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(14445..14831)
                     /locus_tag="AC1Y77_000898"
     CDS             complement(14445..14831)
                     /locus_tag="AC1Y77_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HIQ1574494.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14847..15161)
                     /locus_tag="AC1Y77_000899"
     CDS             complement(14847..15161)
                     /locus_tag="AC1Y77_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HIQ1574495.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(15514..15993)
                     /gene="rlmH"
                     /locus_tag="AC1Y77_000900"
     CDS             complement(15514..15993)
                     /gene="rlmH"
                     /locus_tag="AC1Y77_000900"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIQ1574496.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16421..17710
                     /locus_tag="AC1Y77_000901"
     CDS             16421..17710
                     /locus_tag="AC1Y77_000901"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIQ1574497.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17749..18354)
                     /gene="ytpR"
                     /locus_tag="AC1Y77_000902"
     CDS             complement(17749..18354)
                     /gene="ytpR"
                     /locus_tag="AC1Y77_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIQ1574498.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(18550..19014)
                     /locus_tag="AC1Y77_000903"
     CDS             complement(18550..19014)
                     /locus_tag="AC1Y77_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1574499.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(19036..19356)
                     /locus_tag="AC1Y77_000904"
     CDS             complement(19036..19356)
                     /locus_tag="AC1Y77_000904"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIQ1574500.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(19499..20578)
                     /gene="pepA"
                     /locus_tag="AC1Y77_000905"
     CDS             complement(19499..20578)
                     /gene="pepA"
                     /locus_tag="AC1Y77_000905"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HIQ1574501.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20778..21128
                     /locus_tag="AC1Y77_000906"
     CDS             20778..21128
                     /locus_tag="AC1Y77_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIQ1574502.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21313..22266
                     /locus_tag="AC1Y77_000907"
     CDS             21313..22266
                     /locus_tag="AC1Y77_000907"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1574503.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            22279..24066
                     /locus_tag="AC1Y77_000908"
     CDS             22279..24066
                     /locus_tag="AC1Y77_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HIQ1574504.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            24053..25255
                     /locus_tag="AC1Y77_000909"
     CDS             24053..25255
                     /locus_tag="AC1Y77_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574505.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         7142 a         5130 c         4606 g         8439 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagcttt ctgttaaggt agtttttccc gcatcaacat gagctaaaac tccaatatta
      601 ataattttca tgtgattttc ctccattcaa aagcccaaaa gggcataaaa atcccagtga
      661 taaatactct tatcactggg atttttatgc ataaccatag gcatacaaag catacagata
      721 ttctccggat actttagaat cacatgataa aggtattctt aaactgggtg caaaaaacta
      781 agccctccta aaaaaggaca tccaattatt tgttcccact atcaaattga cagtttattt
      841 aagaatacct tgccgcatat ttattaactc cttttaaata gatacttaaa taatagcacg
      901 taagagcata tttgtaaagg aatctccaat tttttatcaa agagagtacg tgattacaaa
      961 atagctgtaa taatgtacca atatttgtta ttctataatc ttccaattac tcccgttctt
     1021 ttcaagtacc aaatcaaatt gagatacctg cgttgctttg gtctgctggt cgatatactc
     1081 cactgtcagc gataccgtga cttgattatc cttacgattg taaataggat ttaccagttc
     1141 ttgaaagatg tactcttttc cgattggttt taatatcccg tcattcacat agtaggaaag
     1201 ttcactggct gtcgctgtag gatagagctt gaagaacgtc gttaaaaact cattgatttc
     1261 attggttgta atggaatcaa ccgtaccctc actttcaata gcttttggtt tatagtctga
     1321 tttctttggt atgctagtaa tggtcggatt cttaatgagg accatatttc cagtactgtc
     1381 tacatagaca ctcactatat aagcagagtg gacggtcttt gtattttccc cctctgtaat
     1441 gagttggtct acactgtagg ttacatcaaa ttgattgtcg ccatctgctt ctaccgtcca
     1501 tatctgaaat ccttttacag aagacgatac aggaatatct ttacgaactg tatcgacatt
     1561 cagagcttga agttcctctg tcagatatgc tttcaggttt tctatgcgat tatcaatgga
     1621 cttgtcgttt agttcccatg aatagtagac tttcgcaaag ttctctacaa aattttccac
     1681 atgatgagta tcgacatatt ccttttctat gatggtggtt tcatgaatgg tatgggtatc
     1741 aatggctgta aagtgcttga atatcgcaaa gctgaaacta agtcctaaaa gtacccatag
     1801 gacaatcaca acctttttac gaggattgac cttatagaca cgaggtttct tttcctttgg
     1861 tgtctgtttt tctgtattct gattttttcc gaatttcatc attgaatcgt cctttcttat
     1921 tgtttgattc gtcctgctcc cactaaatgc tgttgccagt aagagcttgt taagtcggca
     1981 taaccgatag ggtcgcctgc atgaaacata cggttatcgc ctaaataaat cccgatatga
     2041 gtaatataag agccagcgtt ataggtagaa tgaaagaaaa ccaaatcgcc ggcttttgct
     2101 tccaataatg ggatatgccg ggttacatca tattgctgtt gtgctgttcg tggtaagttg
     2161 attccagctt ttccatacgt ccattgtgtc agtccgctac aatcaaaaga ggtagtcggg
     2221 gaagctccgc catagacata cttccagcct tcaaatttca gagcttcgtc catgatggct
     2281 tgtactgtat catcatcaaa ctctgttgtg acaagatact gcgttaccag ttgcacataa
     2341 aacatattcc cgtaattgta tcgccagccc ccattgatag gtatggctat gggattgggg
     2401 taagacactt tttcgtcacc cgaatactct tttgagaaac tttgagccag ttcaaaggta
     2461 tatttattcc cacgattagc cacatatcct aagaaaccac caccataatt gtaggactgg
     2521 ataaccgatt ctaaatctac accaagcctt tcgctactgg ctaataactc actgaaatac
     2581 ttcacacctt gcttaatgga ttcttctgta ctcaatgaat taggtggaag accgagggat
     2641 tccgaggact gcataacatc ttctgctgta ccacccgatt ccacctgtat aatcgcaaga
     2701 agtatattga cgtattcttc aattccatat tctttggcaa ctttttctac cataggctta
     2761 tgagccagca cttctgcgga aacatccaca ccgccatagt gaatgttgga aaaaccgcca
     2821 tcttgttcat ctgaaaataa aatggcgaca aacagaagca gtgagaagac catcaagaat
     2881 aatcccgaac caccaatcac taaagttttc agcttcatgg tttcttactg cctttcttaa
     2941 tggtggcggt tttgattggt ggtctacttt ttgtattttg tagtggtact ctttgaacag
     3001 tagactgacg ttcttttgtg attgaacgtt gtgaagctct atctgttggt gcagttgaag
     3061 ttactggctt ttgaaccgtt ctttcttgaa ctgtattact ttgacgttcc gtttttaaac
     3121 ttgaagaatc ggacttaact gttggacgct cttgtttggc ttgttgagat tccctatagg
     3181 aagcctgtac agttgtttgc cttgaagtct gtccatcatg agattgttct tgcttagtgg
     3241 ctggtctttc atgaacggaa gaagctggct gttttttctg tttgacctgt tccatttcag
     3301 agcgacgctc cgcaatggtt ttccgtcttt gttcctgctg tttcttgcgt ccactggctt
     3361 tatccgcttt ggtttgagaa atacttctgg ttaaatcatg gacattatcc gtcactttag
     3421 attttccttg atatactgca tatcttgcat tggtcggcaa atccttaacc tgttctttca
     3481 aattaccagc agtatctacc attctgtctt tggtatcagc tactgtaccg atggtttgac
     3541 cgatacgctt tcctagtgtt gatttttcct gcccgtttgg tcgagagtga tctgcttgtg
     3601 tactcgcaga acttcccgaa cccgactgtc cttttttacc cgtagcactg gcaatggcag
     3661 acccagcccc taaagcagtc acggagcgtc caagtttccg ctgtagacgg tgcatgtgag
     3721 cgtgcataag catacgaggt tttctcatca tacgacttcc cacactttga gaatcgttac
     3781 tctgtagaga aaacatactc attaaatcgc ccagcttaaa gtagattcct gcaaaggtca
     3841 caatctgtag aaaagcaatc aaaaagaacg gataaccagc cgataaggta tagagcatgg
     3901 ttgaaatact aaatgctgtc gtaataatca atgtgattcc agctcgtgtc aaaatggtat
     3961 taaagagctt tgttatggct cgttttgaca taccatcaaa tgatggaatc atgcttaaaa
     4021 taaagctcac aggcagaaac atagcataga tgataaaaag tacctgcgag aaaatcatga
     4081 ttcctgttaa taggaataca aatatggaaa tcccaatatt gaagacaaat aggaagaaga
     4141 ctgtacctaa acggttaatc gtctttgtaa tcgtcatatt ggtattgctt ctatcttcaa
     4201 tttcttccgc aacaattttt tctctgtctt ccccattgtt ggaatctgga ctggttgaga
     4261 gcaggctttc cacacggtca ataccaatac tttcaatgtc tgaactgtta tattgaagca
     4321 gtagccacgg ttgctgaacc tgtatggaaa ataggctgtc acgtatcaag tccacactgt
     4381 ccttgccttg actatctgaa tggggcatga caatttttgt acccagtgac aaactagcgt
     4441 tgctgatgtc tgatgaaaag tcattgattt ttttaatgta gtcgggagcg taggcaataa
     4501 aggaagccga taggataaaa accagcacaa aattcatgag ggcatgaatt gcctttgtgg
     4561 tttctctctt tatcagtcct gtataggcaa cataaacccc aagaaccaaa atcaagagta
     4621 aaaggaatcc aacatagaaa ccctctgttg atagtccact aggcgtaacc cctgcaaggg
     4681 tctgcatatt cttgccaatg gaatctgctg tagcggaaat gaagtctaag gaataggctt
     4741 cctgtactaa gtaacccgtc gcattggaaa cataaagact gattgtccaa ataaaattgg
     4801 taatggcata tagtccatac atgacttgtt ttccaattcc gtccgaccag ttccacggta
     4861 gccagcccca gctattatcc acataaaaat ccagttgata gttttcaagt gggtatcggc
     4921 tgtattcatt tgccacattg accgtatcat ctaccaagcc cgcagcttga accaccgttc
     4981 ccagcatggc taaaagaaaa atggcaatca caagtgtgaa agccactgtc attgccactt
     5041 tacctagacg tttcagcgtc cagtttgatt ttattctgtt taatattgat ggtttcacat
     5101 ttacacctct tttcgcacag gtggtctggt atcaaaggca tggagcagtt cttcaaatac
     5161 agggtggaac tgtatcacac cgacacgacc atataaatca ctgataaggc attgcccgtt
     5221 ttccaaatca cgcaatcgct tctgattgtt ttcgtcctct gggtctacac caaaaaaggc
     5281 aagggttttt ttaatctcgt taaggtcagt ggaacgaaag gcaaatttta agccgaggtt
     5341 atttttcagt ttttcatcta agaggtcatc tgtattttgg gtcacgaaat ataccccagc
     5401 gttcatagca cgaccagcac gaaccagctt catagatagt gttttccctt gtgccacctg
     5461 taagaagctc catgcttcgt ctaagtctac aatcttgaaa atacttcggt ctgtatggat
     5521 aaagtccaac gcaaaggtac taatgacaat cagcattgct accgatagta actccattgt
     5581 ggtatattcc tcaaaggaag tttccttgtc aggaagtacc aagtcggcaa cctgtataat
     5641 gttcagttgt ttttcaaggc tgatagactg ctctacataa ccatcactaa aaagcagatg
     5701 ggcaaagtca tagtctgtaa aactttcgat atggtcggct atgctggtac ttagtggcgt
     5761 attctcaacc cgtaattctt caatcacttt catcaaccct cgcacttcac tattggttac
     5821 tgcacgaatg gcttttctaa ggattgggaa gcgttcccca tcacgagagg aaatccccgt
     5881 aaggaatgtc aaaatatcaa tagccagtga ttcagaatct ttggtatttt tcataattac
     5941 ataagggtca agtaagcctt tgtttttctc atcagaagtc agattgacga tattgatttc
     6001 atgggaaatc tctggcaagg tttctttcca tctgccacgt tctgcttttg ggtctacaat
     6061 cactgcttgt gccccataaa gcactgcata atagacgata aggttattcg caaaggattt
     6121 accaccaccc agcgaaccga caaaggcaga cgctaacgca ttggttactg aacccttaac
     6181 cccttgactg gcaagagcag gtttcagata gacattgcgt ccagtatcta agctgtagcc
     6241 aacataaatc ccctcatttt cacccagcat ttgagtagca ccaaaaccta aaccagcgag
     6301 gaaatcagag gtcacgtatt gaatatagtc attcatatat cttttgctgg caggtaaaaa
     6361 ttcttcatgt aagcccagca tatccccaaa aggtcgtacc agtttcacgc tcaaatcatc
     6421 atagaaatct ttcacttcat tacaacgacg tttaagttcg tcaaggtcat ttgctgatac
     6481 ccttacaaca taggacagct tatacataga ttccttgctt tggtctaaat tggtttccag
     6541 ctcgttcaca ctttccagag cttccgccac attagagctg gtttcattat cactttgcca
     6601 agcgtggtta tccaagtctt tcagttcttt ctttttattg cggacagtag atagggcttt
     6661 acgattcgct acaatttcca cattcattga cgtatcaatc ggaaaggtaa attgctgttg
     6721 ctggtagtag aagatttcag aggacgggaa gtccagttcg ccgacaatac tgttgatggt
     6781 aaagtaagct acataggcgg tttcgtcttc ctgctggatt ttcaaatatc gctgtttttc
     6841 ttccaccaga cagcgagtag gcttaatgag gtcataggtt ttaatcagtg tttcatgttc
     6901 cagctttttc tttgatagag gatactcata ctcttcatag gcagtgcctg tctgtccgta
     6961 aaggtgttca atcagatagc cgaagtcatc cttatctaat cttcggattt tgaaacgacg
     7021 ggagatttta ttttctaaaa gcttttccat cttctgaaaa cgcaggattt catcattact
     7081 catactaaca aaatcgccca tcagcttatg gttcacatca tagacaaaat cagtaaaagc
     7141 attttttgct tcaacggtaa gacttttcat agaaaactcc tgatcgttga gaagcaactt
     7201 aaaaccgata aagaaacggt agtccacttg attttcgcca atcatggata ttaaagcgtc
     7261 tgtctgttgg tcgattttgt cataggcaac cgttttgagc tttccagtga cttcattttt
     7321 ggaacgttct tgtgcagaaa gtatgctgga ttctgtactg atttgtaaag catgaatctt
     7381 gccatcacga ttttgtgcga taagctgtct gaaagaatca tgtacttgta ttttctgttc
     7441 cggacttaga aatgagtaat tgtagggaac aagctcatag taagcgtaac attccccatc
     7501 tttattccag accagattgt tttcaatata tttaattgga tatgccataa aattcactcc
     7561 taactgctgt aatggcttct tgtggctggt ttctgccaag cgttactttt tttcctgcat
     7621 aggtcagctt tcgtcgcagt gcataagcaa tgacagactt caaaaatcca taaggctttt
     7681 taccatcaaa ggtttttgta gacataaacc atgtgaaagc cacaggaatc ccaaagtatt
     7741 tgagaaatgc tccctctatc atagaaagag ggggcaagtt gccaagtatc ataactgcaa
     7801 agagtgacac gacaaaccat gtcatttgcg taaaggttat gggaaacgga agtctaaagt
     7861 cattgatgga gtacaacact ttctcaacag accagatact ggtatagctt cgtattttct
     7921 tcatgtaatc aatcctttca taaaaatggg gatagtcttt tgactacccc gtaaacaaga
     7981 aaatctgcca gtaagaatgt accgacagat ttaatagacg atttcaaaaa tcccgtgatt
     8041 ggttgagata aacgttcccg aaaggtctaa atcccgacca taggattcat aatcaatata
     8101 gttttgaaga ctggctggca cttcgcctaa agcacccgtt tcttcaatgt agtagcgtgc
     8161 cacgtcagac atatcatcac aatcggaatg aatgataata tcttcttgat gttcgcttag
     8221 ttcttcaata cttgaaaaat gagtgagcag agcagatagc tccgattgta gttcttctgg
     8281 caactccgat accatttccc ataggcgatt gagttcgcta atagaagtgt attcgtcaac
     8341 cgtaaagggt aactcgtaat catgaatggc gtattcctca tattcatcat tcaagccgat
     8401 tttctctttg acttcctcaa agtcaatggg aaaggtaaac cacgcaccga ccaattcgcc
     8461 ctcattgtat ttgcctaaat tcgcaatata gacttgcata tcgtccatat attcacgtcc
     8521 tttctttgta gagattcaaa aatccctacc gcacttcgtt tggtgtacca ttcctttgcg
     8581 gaacataaga aaaccactta tattccacaa aagaacggtt ttatttaagc accaataatg
     8641 cgattgaata gctctagtaa aatgtctttt actccagcag cgttgaagac taagccaacc
     8701 gcaataatcg caataattaa aaagccaatc agtttgctaa actcacgctt gaagccaaga
     8761 tacaagccaa tcacaacgat tgctaaaagc accagtgatt gagcgtttga tagaaaccag
     8821 ttatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc cccccattca
     8881 cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt gaaaagtatt
     8941 tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc tatatttatt
     9001 tagtcttatg agctggactt tatttagctt caatagagct atatattttt gtattgtttc
     9061 ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa gatttttata
     9121 atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt cattagcttg
     9181 ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata gcgaaattcg
     9241 attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact ggtacatcag
     9301 taatttatca acacttagat tggtgtggat aaattttctt ccctctagag tgtagcaatt
     9361 cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag ggaaaatagg
     9421 ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg ttgcctcctt
     9481 tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac ctaatctgtt
     9541 agtgaagata gccattacag ctctattcag tttcatacaa tccagattta cattcgttgc
     9601 aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg tttcttctaa
     9661 ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca gcttgtcttt
     9721 ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct ttcttctcgg
     9781 tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat ttacaattct
     9841 tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag tgattgctat
     9901 tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat ctgcataacg
     9961 aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt tgcttttaca
    10021 tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat tttcctgcca
    10081 ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta gtggggatat
    10141 tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag tcattttgat
    10201 tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta ttcccatagc
    10261 ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct taatatcaaa
    10321 ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt ttattgcatt
    10381 ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag cttcgcagat
    10441 tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac aaggaatacc
    10501 tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg gcttaggtcg
    10561 atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg tttctgctgg
    10621 caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac tgcctgcatt
    10681 tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa tatccataag
    10741 atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg tgttttgcat
    10801 ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt tatttttagg
    10861 cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac tcgtaatcct
    10921 tattgaaaca acataatact tttctaatat atacacgact tgagggcatt cctccattct
    10981 cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa ctgcttattg
    11041 ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc cgataatctt
    11101 atctatacat atatatcctt aggtcactag ctataagcct gctggcttga taccgcctgt
    11161 aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct acgatttctc
    11221 gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt ttcgtcagtt
    11281 ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg gatacgctac
    11341 ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg gtatctttca
    11401 gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg tcggaggctc
    11461 agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc agttctccta
    11521 ataaccaaac tttttcgttc ttttattagt gccttagctt ttcactaagg aacgagtcgc
    11581 acaaaggttt tgtccaaaat tcataaaaat gttctcctct ctatattcaa tgaatttgta
    11641 tttgagttat ttttttgttg ttatcacgtc ctgttctttt actgactgtt gcttcaaaat
    11701 ctgcttgtgt cggtctgtca gtttcgcatg gtcgagaatg tcttttacaa cctgcgtctg
    11761 gttgatttca tcaagtttaa tcgcaacctt taaggtcggg gcaacttgat gagatagcca
    11821 gttcagcgtc ctttggaagg agtaaggctc tggttttgtg gttagtttta atcgttcacg
    11881 attgttccca ataaaccaag cccattcttc attcagtttc caatcagaac gaggtttgga
    11941 atcgtcttta tctacaaaac ggatataccg attgataatt ttaaaggcgg tatgctctgg
    12001 attgtcatag acgagtaaat cacggactgc ataataggca cgctcatttt tcaatcgaat
    12061 ctcaaaacgg ttttttactt ctgcgtcttc aatgggaata tcatttttct tgtactgctc
    12121 gtagtccttt tcatagatac agaaataaac ttcactttgt aatgaaccga tatagagggt
    12181 gtttcccata cattcctttt cctctttgcg taccagttcg ccactgcgat agcttttaaa
    12241 actgcggaag acggagatac attcttcctg ttggcacttt tcagtgagta cagggatatt
    12301 taaaatccct gtcttatcgt taatggcaag gtcaaggcgt ttcatcacac cgccagccac
    12361 caaaacgtcc ataaagaact cataccagct tctttgttgt gccagaagat agctttcaaa
    12421 ttgtctgcac ccacgacctt tcaattccac cagaactcct ttgtccagtt catgggagca
    12481 aaggacgaat atgtcgccta aagcataatg ctctgaataa gaatagaaac catagtcctc
    12541 atgaagaaaa taggacagtt tcagttgtaa gatgttttcg accacctgct gtacgtctgt
    12601 tgtcggaaag cgaattctta cataatcaaa cagcatttca aggggagcgt cgggattgaa
    12661 gcgttccaga gcttcccaaa gggactgctg taaatcctct gatggcttga cttttcctgt
    12721 ttcaatatcg cttagatact gccttgtaat accagtcgca acagctaaac ggttttgaga
    12781 tagtccataa gccaagcgtt tttcttttaa atgctgtaac caagtttgtt cattcagtaa
    12841 aaatccctcc aatcaaaaag gcgtatgtca acttttaaag cccatttgac atacgctgaa
    12901 attttgtaaa tcccttgtaa ccaaaggatt ttctaatgtt tttttgactg tttcctgtcg
    12961 atttgtaccc ccctgttaga taccgagggg ttaagtgctg gcgtggctat tgccacacca
    13021 gccagcaaga tcagtccaca cctgcgactt ccgcttcgca cgtcgcctgc gtggactgtc
    13081 tgctgttgga taacttttta atttcctcca agaaatcata tccttttggt acaaggggag
    13141 tataaaactc tgatatgaca cttgttccta catcaacata gccacgacct ttgattcgct
    13201 ttaagaagaa atccttttgt acgtcactgc caaacatcat gccatagccc atttcagaca
    13261 tacgacctaa agccactctg aaattaaact gatcacggat tccgtcgcct aaatattttg
    13321 cgtctggacg ttgacaagcc agtattagaa agaagccagc ttgacgacct aacatgacaa
    13381 tctgtttcag cttattcata actgcggtgt tttcttttgt tcccagcatt tccatgaaag
    13441 cgacgtattc atcaaagatt aagaagtgtg ccgggagacc taagtaagca taatttttgc
    13501 cagtcttata gttcttcatc tgcttcattt cctcactacg tttcatcatt tcttcataga
    13561 atgtttcaat gcaagaaagc aagtcttctt ttctatagta gacatttgcc atcacagaac
    13621 ctaagtccgc aaggtcagca tttttcgggt caagaatata cagttttgaa tctgtatgaa
    13681 gcaaggcttc aatcagtgtc agtataaagt aagttttacc gccacctgta ccaccagcaa
    13741 tcaacatatg agggagctta tcatattccc accatacgtt tttcattaag cgaagtttac
    13801 catctttagc ttctacttca tcaatagaaa tacgactggc tatggtgtca tagagcaaag
    13861 tatattccac ataggaatcc tttaactctt tatccgtcag ctcacagtac aagccactct
    13921 ctaatttctt ttccaagtgt aagagttggt cttgatattt tcccagcgtg atttccaccc
    13981 gtatctgtat caagccattt ttaagtcgat aatacatttt agggaagtag gttatctttt
    14041 cctttgtacg accagcacta tctttaaaga aaccctctgt tttgacctgt tcagattcat
    14101 accacttgtt ttcaagtatc atctttgcca gtttttgacg gtggtaaagt tgtttaaccg
    14161 tatcatagcg aacccgtttg aatacaaacg ctaccagcaa gcagataaga attgcgacac
    14221 tgaaactgat aattaaatag ggaatgtcaa tcttatctgc ttgtgatagg ttaaaatcct
    14281 gccagttgat ctgctggatt gtcttcacat gaaacagtcc gacaaccagc aggaaaacag
    14341 gcaggagtga cgctatcgta aaatgaaaga ctaaatcttt accagatggg cgaatccttt
    14401 taccacgctg tttcatgcga aaaagtctcc tttctaccta gcgactattt gtcttgtgtc
    14461 ggttctttct ttgcttgtgg ttgagctttg aatgaactag aatcctttgt cagcacaata
    14521 tcgtctgcct tgatatacca gtcaacatct gctccttgat aggtggcagt agcaacggtg
    14581 tccgcaatgg gattgataag ttccacccgt gcgttataat caaactcttt caaaggcacg
    14641 ctggcaggaa tacttacttg aatcatgcgt ccttgtcctt tggattttaa gtcataggta
    14701 cgttccttga tttcatctga aaccgacccg tcttcatttt ggattctcac ttcacgacgt
    14761 agagcagaga atttcaattc tccaaaagtc gtgtctttat ctaatacaat gccatttgct
    14821 aatctcatca tttttcctct ctttctttat tcttttatca tgtcgtcagc atgtaaaagg
    14881 taatttgtaa aaccacgagt gccgattttg tagccctctg cggtaatacg tggattgact
    14941 aacttcacac gttcctcaaa gccgaaatgt ttttcgccag cttcagcagg aagcaccacc
    15001 acaatatcat ctgctctttg aacatcagaa tagagattat agcttcttga taagacagtt
    15061 agccgtccgt tgattcttcg ctgaacgact ttatcctcgc cagcaaattc taaattgccg
    15121 aatgtttttt ccatgttggg aatcacaaat ttaagttcca tatttttacc tatcctttct
    15181 tttttattgg ctgaatgaat gtttgatggt cttaaagagt ggggaacgac cttttgattc
    15241 ttgatttttt gttttcataa gttcacttcc tttcaaaatc gggtaaaaaa atagacacct
    15301 cattttttga agtgtctacc tattaaatat tcaaatttta ttggaagtat ctttatatct
    15361 tcacttttca aggataaatc gtcgtatcaa agctcattca taagtagtaa attagtagta
    15421 aattgagtgg ttttgacctt gataaagtgt gataagtcca gtttttatgc ggataactag
    15481 atttttatgc tatttttctc ttaaaaaata tcattatttg tggtatggag cccccgcgtt
    15541 gatccggaag gcacggtaga tttgctcgac gaggatcagg cgcatcaatt gatgtggata
    15601 cgtcattttg ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc
    15661 taaagaaccg ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat
    15721 ttgtttagca aaagattcgc ttgtgggatt tttcccttca attgctaagc catagacgta
    15781 ttcttgttct ttgattttcg ctaagatccg ttctccttct ttttctttta cttgtctcat
    15841 ttgcgcttcg cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt
    15901 cgcataggca ttcagccgtt tgacatactc attgatacct tggatcaagt atttttcttt
    15961 taattttcct actgaaataa ttttgatatt catgttttct ctcctttgat cttcacgctc
    16021 actttgttta ttataatcaa ttttttcgtc tatatgttta ttttgactat ttttgttaaa
    16081 aagatttccc caacagttat gcacaacatc cacaggttta tccacagaaa acagcgttgt
    16141 tttaccttat ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt
    16201 tatacacagg ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt
    16261 acacaatatc tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct
    16321 aaagtttttc ataaaaagtt caaagtctca aagtagacta aagtcaacaa ataagactat
    16381 actaacagta gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac
    16441 accaaagatg aaaaaaaata aaaataatag cctctggcgt aagttgggtc tggggcttgt
    16501 tggagggatc gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg
    16561 caacaatagt tctactgcta caagcggcaa tcaaaattct gccggtgaaa cagttgtgga
    16621 aaatgtcaaa gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc
    16681 tgtcgtttct gttatcaact tacaaagtca aaaccaatca agcggttttg gcggattatt
    16741 cgggcagcaa gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg
    16801 aagcggcgta atctacaaaa aagacgggaa tactgcttat gtagtcacaa acaatcacgt
    16861 agtagatgga cagcaaggtt tggaagtcat gatgaaagac ggtacgaagg taaaagctga
    16921 attagttgga acagatgcct attcagattt agcggtgttg aagatcaatt cagacaaagt
    16981 cgaaacggtt gcctcttttg gggactcaag tgctttgaag gttggtgaac ctgcgattgc
    17041 gattggttcc ccattaggat ctgaatatgc gaattctgtc acttcaggaa tcatttcttc
    17101 cttgaaccga caagtaacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat
    17161 ccaaacagat gcagcgatca acccgggaaa ttctggtggt cctttagtga atatcgaagg
    17221 acaagttatc ggtatcaact caagtaagat cgcaagtact tctgaatcga cttctaatgt
    17281 aagcgtagaa ggaatgggat ttgccatccc aagtaacgat gtagtgaata tcatcaacca
    17341 actggaaaaa gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc
    17401 tgccgtttct actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg
    17461 tgtcatcgtg acaaatgtcc aaacagctac accggcagaa aaagctgggt tgaaacaata
    17521 cgacgtcatt actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc
    17581 tttgtataag aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga
    17641 aaaaacaact actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag
    17701 tgaaaattaa taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa
    17761 tacttctccg actacggcat cctcaggcaa tactaagatt cctttttttt ctggagcatt
    17821 tggcaaatgc aattcacgag cagaacaaat cattccatag ctggctaccc ctcgcaaagt
    17881 tcctggccag atcatcattc catctggcat catggctcct ggttttgcaa cgacaacttt
    17941 ttgtccagct tcgatatttg gtgcaccgca aacaatttgc aatactgtcc cattgtccac
    18001 ttctgtctca gtaatcgata aatggtcaga gtcaggatgc gggacgcatg tttttacata
    18061 cccgacaacg atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt
    18121 aatcaatcga ttcaatttcg ctaattcttc atctgttaag cttacttggc cgttcccttc
    18181 aattgttaga tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc
    18241 tttgatccga gcaacattcc atttacgttc gacagtgttt tctgttcctt tatcgtcggc
    18301 tatgataacc atcaaagtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt
    18361 tcctccatta tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgagat
    18421 ttttttcatg ctttatttcc gaagaagctt cttctggaac accatttatt cgtaacaagg
    18481 gactgtgaca agacttttgt cacagtccct ttactgtttg attaaaaagg ttatacaagt
    18541 tccacttgct taattactcg gctgttcttc ttcgtcagaa acgatcaaga catcacaagg
    18601 tgaatgacgt atgacgtagc tggctacact tcctgtcata aagcgttcga ctgcatttag
    18661 tccagactgc ccaaccatga ttagatcgat tgcatatttt tttggcaatt tgactgccat
    18721 ggattcacga acagagccgt atgcaataat tccttctata tttgtgtaat caacagattt
    18781 tccatattct ttacattctt cgatcatttg cttggctgtt tcagtttgct gatcgactag
    18841 attttgatca ataggagcat agcccataaa attataaaat tgctgatcga ttacgtttgc
    18901 tacatatact ttcccattat tacggcgagc cacttctaac gcttttttga aagcagcaag
    18961 tgcttgttca ctaccgtcaa tacctactaa aatgttctta tattcttgat tcatcttgac
    19021 ccctcctaac agatgtcagc cttttattac actttctagg aacgattcga tttcctgttt
    19081 tgttttacga tctttattca ctagacgacc taattctttt ccgtcttgga tcacgacaaa
    19141 acttgggata ccaaaaatgt tccattcagc ggctacatct atgtacttgt cacggtctac
    19201 ttcaataaat tgccaatcag aaaagtcatt ttcgatttca ggcatttgcg gtttgataaa
    19261 acggcagtct ccgcaccagc cggcagtaaa gaaaaataca ctttttccag tttctacgta
    19321 gcctgctaat tcttctaaac tatttgggat aatcattctt tttcctctac tttctgttta
    19381 tgtatttttc aattaaatta tacacctttc tttcgaacta acaaacattg atcctctttt
    19441 tataaaaaaa atctgacaaa aggttgcttt tgccagattt tatagtttca ctatcgcgtt
    19501 atttcccgta tacgatcgtg ttcactgttg atttatctaa acctttcaat gtttgaatca
    19561 acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc gtatggatat
    19621 aacgtcccac tacgccaatc actgtacttg ggattccttc attttgtgta tgagcagcac
    19681 ctgcatctgt accgcctttt gaaacaaagt attgataagg gatgttgttt gtttcagcga
    19741 tatctagcaa gtactcacgc aaacgaggaa gcatgatcaa acctggatct tggatacgca
    19801 tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgtg tcatctgctg
    19861 cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa gctttagaac
    19921 cacgcaagcc tacttcttct tggacattcg cgcctgcaat caatgtatga cctaatgttt
    19981 cattttgtaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg ttgtcccacg
    20041 atttgctgat gatattcttc ccgttggctg ttttgatcgt ttcagcgtaa ggaacgattg
    20101 tgtctcctgg aagaacaccg taactcattg cctcttctct tgattcaaac ccagcatcga
    20161 acaaaatatc tgttacttct actgattttt gtccgcttgt tcctcgtaat aaatgtgggg
    20221 gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt gtaaaacgtt
    20281 gagcagaaac aacgtaaggg ttccatccgc ctagaggtac gactttaaaa agcccgtttt
    20341 cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc attacacgag
    20401 gtgcatccgc ttcttcatgg tggcgcagcc caaaaatgcc gcccaaacca tctaactgta
    20461 actcatctac cagcggttcc atatgacttt ccatataggc gcgaacgtct tgttcaaaac
    20521 cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt tcttccatat
    20581 gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct gcggataaaa
    20641 tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg gtagcggtta
    20701 tttatttcaa agaaaatatg agagtgtggt aaaataaaca caaagagtag caagtgagaa
    20761 gaaggaggta tatccacatg tatgaagaaa gcgaattaag ctattttaaa ggcgggttag
    20821 ccgttgggat cggtctagga ttattgagtg gggtagcttc aacgatttgg tatcatcgca
    20881 aaaaagtgtt atcagcagac caagtgcttg aaaatatcaa aacatctttt ttaaaagaag
    20941 gcgcaatcga aggtgcttgg atctcttttg aaaaagaacc tattcggaaa ttcgccgttc
    21001 ggtcgagtgc ttatcggggt gggctttccc ggatagaaga tggcgaaact gtttattatg
    21061 aatttttagc agatgctcat acaggaaccg tattagacat ttcaagaaaa aagggcgcaa
    21121 tagattaata cgtcaaaaaa atgaaataac gaatacgcac ccactatcta aaagagaggt
    21181 atcctatcct tcttttatag tgggtgattt ttataaaaat agggctattt cctcttggaa
    21241 tagctagtat ttgtagttat tatacggtat tcttgaaata agattagttt tattatataa
    21301 ggagagtgac aaatggaatc acgtgaaaaa attgccgtgc tcattccttg ttataacgaa
    21361 gaggcgacaa tttcaactgt tattgaagat tttaaaaggg aattaccaga agcagatatt
    21421 tatgtctatg ataataattc gaccgatcga acatacgaat tggccgttca aggcggagca
    21481 atcgtaaaga aagaaccacg tcaaggaaaa gggaatgtaa tccgtcaaat gttttttgat
    21541 attgatgccg attattattt gatggtagat ggtgatgata cttatcctgc agaagctgca
    21601 catggtttgc ttgagaaatt acgttctggc gaagcagata tggtcattgg cgatcgtcta
    21661 tcaaatggaa cctattttga tgaaaataag cgtcctttcc atgattttgg gaataatctg
    21721 gttaaaaata caattactta cttatataaa acaaaaattc gcgatgtcat gaccggctat
    21781 cgaggattca atcgaatttt tgtcaaaagc ttccctatta tgagttctgg ttttcaaatc
    21841 gaaacagaat tgacgattca tgctttagat aaaaaattca agttcgttga gattccaatc
    21901 gattatcgcg atcgtcctga aggtagcgaa tcaaaattga atacattctc tgacggattc
    21961 aaagtgatca tgatgatcat caaaatgtgc aaagattata aacctttgct tttctttggg
    22021 atctggacca tcttcttctt tttatttgga ttggtcatgg ggattcctgt gattcgtgaa
    22081 tatatgctga caagctttat tacacgcatc ccttctgcga ttttaagtac tggattgatg
    22141 actttagccc ttctttcatt agtaacaggg ttgattttgg atacggtcgt cacaaatgcc
    22201 aagaaagaat atgaattgaa tttataccat gtctaccacg aatatcaaaa taaagaaatg
    22261 aagtaggagg ccttttttat gcataagaaa aaggagaatc tatttcctac tttttttgca
    22321 actttatttg cgtcaggttt tgcttttttt acttcttatg gtctaggaac tttgaatcca
    22381 acagtaatca ttggtttagc catggttttg ctacttgctt ttaagcaata tggaatagag
    22441 ttgttttcaa taaaaaacaa agttgcagct attgcccgat tgtttttttc tgcagctttt
    22501 gctcttacac tttctattgg cagacagata caatttcagt atgtctcagc aaactataca
    22561 aagacaacga ttgctgatgg aagtattttt tactttcttg tttatttctc catttttgtg
    22621 tattcttttt tatttttaca atcgatttat ttatcaatgc caaaactgga aacatttttt
    22681 actgctcaaa acacacaggc tttctcttca agaaaatatt ttttattctt tactgtcttt
    22741 ttagttctgt gttggcttcc ttatctgttg gcattgtatc ctggagtggt tttgcctgat
    22801 tcgttaagtt cggtcgccca atctatgggt gatgttccta taagcaatca tcatccagtc
    22861 ttattcacct tactggtcaa gctatttgtt catgatttac caactgatac gatcaatcac
    22921 ggtgtatttt tattttcgat ttttcaaaca ttgataacag cttcagcgat aagttattct
    22981 ctttgctggc tgttaaaaag aaacatccat ttttttccgg tactcattag tttatgctat
    23041 tttgcatttg caccggtttt tccaatctac gccttgaaca tgcaaaaaga tactttgttc
    23101 tctatcgctt gctttttatt ttccttagcg gtttttgact ttatttttca acgagaaaag
    23161 ccaacgattc tcaatcagat ttacgtaatc atccttgctt tactgaccac ctatctaaga
    23221 aataatggga tttatgtagt aaccggcaca ggtttgattt gcggcttgat tattttgaaa
    23281 caaaaaaggt atcaagcatt ctttcaagtg gtgctgggtt atacagtgat tgctggacta
    23341 ctcatccacc cgttgatggc tcgttatgct attcctacag aaactgctga acaattaggt
    23401 gttcctttgc agcaaatttc tagaactatt gttatggatg gtgacttgtc taagaaagat
    23461 aaagcattca tgaatcagct actcccgata aaagattatg aagcgtattc tcctagtctt
    23521 gcagactcaa tcaagtggca taaaaatttt aacaatgagt ttttgaatac acataaaaaa
    23581 gaatttctat cactatgggc aagaaattta ccaagtaata taaaaatcta tacagatgct
    23641 tatattttag aaacgttcgg gttctgggta cctggtacaa aaaatagtta cggcttttta
    23701 gatactcgtg taaacacgaa taattatggt atgaagcaag tagatttgat tgaacatctt
    23761 actggattgt caggaatgaa aaaaatcatc gatgctcgtg atttctttgg ttccggcaca
    23821 ttactgtggt tagctatcgg cagtgcactt ttagcaatct ttaagaaaaa atatcggtat
    23881 ttactccttt ttcttcccgg tatgcttgtt tttgctacta ttatgattgc tactcctgtg
    23941 gcttttagtt tacggtatgt gtttgtttta gcattaggtc tacctgttta tatcctctct
    24001 ccttttttac tagataaaag agaaaaaatg aaaacagata taggagatac tcatgaaact
    24061 atttaaaaag atattttttg cttttgttct cttgatcgct agctattgcg tactactgac
    24121 aattactttt ttcattccgc agcactctat tgaatcgaat gcagaaaaat ctctacagat
    24181 ggttgaaaaa gaaggaatgt atccttctat caatcaagga aatatgctag gcactcggtt
    24241 agataatttt actgatcatt tgatgatacg aaaaacaaaa gcagatcctg aattgaactc
    24301 cttggagaac gcgatgtcta tggcggatta tccccgttat tggcatggtt atcagctttt
    24361 tttacgacct ttactgctag ggatgagttt ggggagtatt cgaatgattt acgcagccat
    24421 tctttttctg ctgattggat tgacgagcta ctttttaacc aagtgtagcg acatctattt
    24481 aacgacagcc ttgttgattt cgcttgctat aggaaatgct gcaacctttt tcttcagtat
    24541 gcaattttca aatatttgga ttttgacttt attgtctgta ttactatttt taacaaaacc
    24601 acaactctcc aacaccaacc aacaattatt cttgtatttc tttgtcattg gttctttagc
    24661 taactttttt gacttgttga caacaccggt catctcttgg gggatacctg tgatcattat
    24721 ttattatgta acaaataagt atttaatgga taaacgcagt tcattgagca aacaagtagg
    24781 cagttttgtt tttaccggta tcttttgggg attaggctat ggtttgacat gggtggctaa
    24841 atggatccta tcatcgatca tattgaacaa aaacattgta aaagatgcaa tcaaccaaat
    24901 cctctttcga acggaaggaa acgacaaata cccgttgcat cgattagaga tgctgaaaag
    24961 caatattaga ttgatgtatc caaaagtagc aatattattg ctattgatta cttgtcttgt
    25021 ttttctctat tttgcttatc gtaaacgaaa tagctggcgt ccgttccaac ttctagtttt
    25081 gtttgtttta gctgctgtaa cgccgtatat ttggtacaat attttagcta atcactcaca
    25141 gattcattat tggtttacgt atcgtacgca aatcatcaca agttttgcta tacttagtgc
    25201 tttcgccttt ttgatccctc ctgagaaatc aaaagacgaa ctaaattact tttaagaata
    25261 ccgatgtact aattcatttt taaacataaa aactccagct gtcagagata gccggag
//
LOCUS       DBNIBO010000026        24304 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.026, whole genome shotgun sequence.
ACCESSION   DBNIBO010000026 DBNIBO010000000
VERSION     DBNIBO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.026"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(6..1373)
                     /gene="nhaC"
                     /locus_tag="AC1Y77_000910"
     CDS             complement(6..1373)
                     /gene="nhaC"
                     /locus_tag="AC1Y77_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIQ1574506.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1482..2879)
                     /gene="tyrP"
                     /locus_tag="AC1Y77_000911"
     CDS             complement(1482..2879)
                     /gene="tyrP"
                     /locus_tag="AC1Y77_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HIQ1574507.1"
                     /translation="MGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIFAVLFFAGP
                     ISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVASTLGPLLG
                     NTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYIPVVIMFVL
                     GVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMSSVYMPRLK
                     DATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDCQILGLPEV
                     IGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYGVSRNVVLT
                     QSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKKHPEFERPY
                     RIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVIPLIINHFK
                     KDSWGIEVEKSLEEK"
     gene            complement(3146..5023)
                     /gene="tdc"
                     /locus_tag="AC1Y77_000912"
     CDS             complement(3146..5023)
                     /gene="tdc"
                     /locus_tag="AC1Y77_000912"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HIQ1574508.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5314..6570)
                     /gene="tyrS"
                     /locus_tag="AC1Y77_000913"
     CDS             complement(5314..6570)
                     /gene="tyrS"
                     /locus_tag="AC1Y77_000913"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIQ1574509.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6682..6887)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7150..8451
                     /locus_tag="AC1Y77_000914"
     CDS             7150..8451
                     /locus_tag="AC1Y77_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIQ1574510.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8520..9119)
                     /locus_tag="AC1Y77_000915"
     CDS             complement(8520..9119)
                     /locus_tag="AC1Y77_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1574511.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9374..10255
                     /locus_tag="AC1Y77_000916"
     CDS             9374..10255
                     /locus_tag="AC1Y77_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1574512.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10268..11878
                     /locus_tag="AC1Y77_000917"
     CDS             10268..11878
                     /locus_tag="AC1Y77_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574513.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFILGSIGYRRRDLLEG"
     gene            complement(11955..12614)
                     /locus_tag="AC1Y77_000918"
     CDS             complement(11955..12614)
                     /locus_tag="AC1Y77_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIQ1574514.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12897..13562)
                     /locus_tag="AC1Y77_000919"
     CDS             complement(12897..13562)
                     /locus_tag="AC1Y77_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HIQ1574515.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13709..14314)
                     /gene="dhaL"
                     /locus_tag="AC1Y77_000920"
     CDS             complement(13709..14314)
                     /gene="dhaL"
                     /locus_tag="AC1Y77_000920"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIQ1574516.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14342..15352)
                     /gene="dhaK"
                     /locus_tag="AC1Y77_000921"
     CDS             complement(14342..15352)
                     /gene="dhaK"
                     /locus_tag="AC1Y77_000921"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIQ1574517.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15327..15722)
                     /gene="dhaM"
                     /locus_tag="AC1Y77_000922"
     CDS             complement(15327..15722)
                     /gene="dhaM"
                     /locus_tag="AC1Y77_000922"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HIQ1574518.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15876..16196
                     /locus_tag="AC1Y77_000923"
     CDS             15876..16196
                     /locus_tag="AC1Y77_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIQ1574519.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16308..17165)
                     /locus_tag="AC1Y77_000924"
     CDS             complement(16308..17165)
                     /locus_tag="AC1Y77_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIQ1574520.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(17273..18577)
                     /locus_tag="AC1Y77_000925"
     CDS             complement(17273..18577)
                     /locus_tag="AC1Y77_000925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1574521.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18574..19083)
                     /locus_tag="AC1Y77_000926"
     CDS             complement(18574..19083)
                     /locus_tag="AC1Y77_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574522.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19258..21393)
                     /locus_tag="AC1Y77_000927"
     CDS             complement(19258..21393)
                     /locus_tag="AC1Y77_000927"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HelD family protein"
                     /protein_id="HIQ1574523.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21476..23131)
                     /locus_tag="AC1Y77_000928"
     CDS             complement(21476..23131)
                     /locus_tag="AC1Y77_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1574524.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23133..23447)
                     /locus_tag="AC1Y77_000929"
     CDS             complement(23133..23447)
                     /locus_tag="AC1Y77_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIQ1574525.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23617..23877
                     /locus_tag="AC1Y77_000930"
     CDS             23617..23877
                     /locus_tag="AC1Y77_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIQ1574526.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23891..24205
                     /locus_tag="AC1Y77_000931"
     CDS             23891..24205
                     /locus_tag="AC1Y77_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIQ1574527.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6934 a         5020 c         4387 g         7963 t
ORIGIN      
        1 ttccttcaat tttttacacg tcgacgattc aataaaaaac cgcctaatat tgtaaagaca
       61 ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca acgcgctaac tttcatcgtt
      121 cccataatga atgtgccgct gactccccac ggtacgagag aattgacggt cgcacctgaa
      181 tccgctagtg ttcgtgtcaa ataggttttt gataagccaa cgtcatcaaa agaacgcttg
      241 aatgtttctc ctggaagaat gatggacaga tactgttctc caacgatcag attgattccg
      301 atacagctca aggcagtaaa cccgactaaa cgagaaggtc ggtccatctt ttcttttaat
      361 tcttgaacga tggtatcaac gatcagatat ttgatcagta acccacctaa tgccaatgca
      421 agtaaaatca aactagcaga acctaacata ctcatgatcc cgcctcgaga aagcagttca
      481 tctagctttg gatcaggtgt atttgcgaca taaccattca tcaagatgtc tgctgctttt
      541 ccgaccgtca aacttttctc atggataaaa gaaagaacga gagcagctgt cgatcccgct
      601 agtaatgtag ggatagccgg aatttttttg attgcccccc caaataaaat aactagtgga
      661 atcaatgtcc aaggagagat ccaaaagctc gctttcatgt tttggatcat ttcattgata
      721 ctagctagat cggctgaatg actgttatgt cctaatatcg tataaaaaat caacgaaagg
      781 acgaatgctg ggaaatccgt ataacgcata ccagcgatat gttcgaatag attaactcct
      841 ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg agcagttatt ccctaaaaag
      901 gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga atccaagtaa atgaccgatc
      961 cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg aactgccgac agtcactccg
     1021 acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac aagtttaaat
     1081 ccataaacca taatcgtagg gatggttcca gataaaatcc atgaagcaac taagacacca
     1141 atcaaaagaa aaatcaggat tggaatgatc cctggagtga ttcctttgat gatcccgtca
     1201 tggatctcat cccatgtgaa ctttttaaat ctgccataaa acaacaaaca catgaaagct
     1261 gcaaggatcg ggatatgtgg cgagagctgg aatccgatga tcagtactcc taggagtgcc
     1321 aacaacacga tcaatacagc gatactttct tttaaagaaa tatttttatt catcgtcgat
     1381 ctcctatttc aaatgaaaag gccctgccat gaaaaaacta ttcacttggc aggacctcct
     1441 actaaatcag cgacaaaacg tcggctgaaa gacttatttt tttatttttc ttctaaactt
     1501 ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg
     1561 aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat
     1621 gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat
     1681 ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt
     1741 ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt
     1801 gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat
     1861 gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta
     1921 tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat
     1981 ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag
     2041 atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg
     2101 atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg
     2161 acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt
     2221 gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg
     2281 ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat
     2341 gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct
     2401 aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg
     2461 acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata
     2521 caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac
     2581 aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc
     2641 catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg
     2701 ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa
     2761 aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga
     2821 atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt
     2881 ttacttactt cagatttact caccgttgac actctccttg attttttgga taaaataacg
     2941 gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc
     3001 tttgagttta ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga
     3061 gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag
     3121 ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt
     3181 agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt
     3241 gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg
     3301 cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc
     3361 catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc
     3421 ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt
     3481 catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg
     3541 gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga
     3601 aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag
     3661 gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca
     3721 agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa
     3781 agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg
     3841 gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat
     3901 atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt
     3961 aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg
     4021 cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt
     4081 ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg
     4141 atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga
     4201 ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt
     4261 ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg
     4321 gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga
     4381 tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt
     4441 tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa
     4501 gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat
     4561 cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta
     4621 cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc
     4681 atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac
     4741 cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt
     4801 ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt
     4861 ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat
     4921 ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga
     4981 atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa
     5041 aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag
     5101 ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt
     5161 ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta
     5221 ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta
     5281 tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta
     5341 atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat
     5401 gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc
     5461 ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt
     5521 ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc
     5581 cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt
     5641 caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc
     5701 acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa
     5761 gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg
     5821 gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt
     5881 tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctgc
     5941 ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc
     6001 tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga
     6061 ttgcaggatt tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac
     6121 gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc
     6181 aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt
     6241 accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat
     6301 cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc
     6361 gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg
     6421 aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat
     6481 gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc
     6541 gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga
     6601 aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa
     6661 aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg
     6721 tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa
     6781 gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc
     6841 tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt
     6901 gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc
     6961 ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta
     7021 aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc
     7081 tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg
     7141 acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc
     7201 gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc
     7261 attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta
     7321 gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg
     7381 gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag
     7441 gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac
     7501 agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat
     7561 tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt
     7621 gttttagtta atttgaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac
     7681 gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat
     7741 tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa
     7801 atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct
     7861 ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc
     7921 ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga
     7981 gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag
     8041 aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca
     8101 ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga
     8161 aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca gccttattca
     8221 gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc
     8281 gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc
     8341 aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca
     8401 ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc
     8461 atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt
     8521 tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga
     8581 agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg
     8641 atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa
     8701 agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct
     8761 tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga
     8821 ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt
     8881 tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca
     8941 taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg
     9001 ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca
     9061 aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt
     9121 tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg
     9181 tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat
     9241 tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaaag ttttataaaa
     9301 aggtataaaa cctttttatt aaactattta atcttgttta gtatatattt ttagaaaaag
     9361 gagtggggtt tttatggcag aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg
     9421 tttcgaagct ttaaaagatg tctctttcac cgtacattca ggtgaagtcg ttggatttat
     9481 cggaccaaat ggttctggaa aatccacgac gattcggaca cttttaggga ttataaaaaa
     9541 agacggcgga acggcaacga tttttgggca agatgtatgg gaagatagtt tatctatcca
     9601 tgaacgtctt tcttatgttc caggcgatat ctctttgtgg ggaaatttga ctggaggagc
     9661 gatcatcgat ctcttcatga agcttcatgg aaaagggagt gctcaaaaaa aagatgaact
     9721 gattcaacgt ttctctcttg atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg
     9781 ccaaaaagtg ggcttgattg ctgctttggc tgttgattcg gatctctatt tatttgatga
     9841 gcctacttct ggtttagatc ctttgatgga aacgattttc caagaagaag ttgaaaaatt
     9901 gaaagaacag ggcaaatcca tcctactttc ttctcatatt ctcagtgaag tcgagcgact
     9961 agcggataaa gtcgtgatca tacgcgaagg aacactcgat gaagaaggaa cactcgatga
    10021 attgcgtcat ttgactcgtt cgaccatcac tatagaaaca gaagcagaga atagtcactt
    10081 agcacaattg aacggtgtcc atgactttgt gcaaaaaggt cacctcgctc aatttgctgc
    10141 tgataacgaa gctttcgatg tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt
    10201 tgaatctatt ccaccgactc ttgaggattt attcatgaga cattatgaag gatgaggagg
    10261 aagcgaaatg actgataaat tttcacgctg gtccaccctt ttttctcaaa accttagccg
    10321 tgactggaaa aagatcctga tatggctagt cggtgtcact gctttttctg gtggttttgt
    10381 ccctgcgttt gaagaattga cttccggaca aggattagct ggattatacg aaaccttgca
    10441 aaacccggca atgattgcaa tggtcggacc aactccagct tcgaatgctg ctgagtatac
    10501 tttaggagcg atgtatgcaa atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat
    10561 ctctgtttta cacgtggtga gccaaacgag aaaaaaagaa gaacttggtc tgattgaact
    10621 tattcgttcc tttcgagtcg gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat
    10681 tctgatcaat ttgcttttag caatatcgat cagctgtacg atgatttttt tccaagcgga
    10741 ttcgatcact gcaaaaggtg cttggctttt tgggctatct gtaggtagta tggggatttt
    10801 ggggagtgcc gttgcactgt ttgctgcaca actgatgcca agttcagcca gttcgaatgg
    10861 tctgtcttta gcgtttttag gtggacttta ccttctacga gcaataacag atgtagctga
    10921 ttcctccctt tcgatgtgga atcctttagg ctggtcttat ttgacctatc catttacaac
    10981 taacaactgg attcctttgc tttatttgtt tttgttcagt ctaatcttga taatcagttc
    11041 atttgtttta gaggaacatc gagatttgaa caccggctat atccccgaac gtgaaggaaa
    11101 accgtatgct aaacgttctc ttttatcctt gcgtggttta ttattctatc taaataaagg
    11161 gatgacaatc agttggctta tcacttttgg tttgatgggg gcggcatacg gctctatcta
    11221 cgggaatatg caacagtttt tagaaagtaa cgacttaatg aaacagatgt ttatccaatc
    11281 tggcgtctca attgaaaaaa actttacaag cacgatcatg gtagtcttga tttgtctagc
    11341 tgctatttta ccaattgcta gcatcaatcg tttatttaca gaagaaaccc acggacgtat
    11401 gaatcagctg ttcgcaacaa aaatttctag gaaccaactt tattggacca atatgttact
    11461 agctattcta tttgctatat tggcaatcct gattagttct ggtgcactag ggctttctgc
    11521 tttggcttcg atgaaggatg cgaaagacat ggatctattg atgtttctta aagccgggtt
    11581 caatttcctg cctgccgttt tagtgtttat cggaattagt ggctttctat tcggatggct
    11641 tcctcgaatc ggtaaacttg cttatatcta cttagcttac tcctttgcac tcaattactt
    11701 tggcggaatt ttggatttac cagaatggtt ttccaaaaca gctgtcccta gctgggttcc
    11761 acggatgcca aacgattctt ttgatctttc cgtgttcctt acgttatcat gtatcagcct
    11821 tgcactgttc atccttggtt ctattggtta taggcgaaga gacttactag aaggataata
    11881 aaaaagcatg tacaatttca tgctagcctc caaatctggg tcaaaaatcc aacatttgga
    11941 ggtcattttt ttatttacaa gtcttctaac tgctctttat caatgatcca gacttctcga
    12001 ttctttcttt ttacataacc attctcctct aataatttaa attttctaga taaagtttcg
    12061 ggggtcgtac cgataaatgc agcaagttct ttcattttca ttggcaggaa aaagtgatcc
    12121 ttccctgtcg ctttagctaa gtcaagcaga tagacagcga ctcgttcttc tactttttcc
    12181 atcatcaaaa aagttgtctg ctgctgtact tttgttaatt tctcaacgtt gattgctaaa
    12241 agtttcagac taagggcggg ataatgcagt aagagttccg agaaatcttt ttgtctcaat
    12301 atgcaaattt ccgttttttc tagtgcttcg ccaaataaag actcatttgg aacgcctagc
    12361 aactggctct ctccctcata tccgcccggc tctattactc gtaaaagctg ttcctttccg
    12421 ctagcagata attgatacac tttcatactg ccggaggcca cgatcactaa ttgaggttct
    12481 ccattcggcg caagaatctg ttctcctttt tccacatagt gatgtttgac aagtgcgtgt
    12541 actttttctt gatcttgctg tactaagtga ttgaacaaag gaacaaatga aacacataaa
    12601 tgatcatgac tcataatctc tcctccttct aaacagctat ttctaataaa gaaacataac
    12661 ttcccttatt ccaactgata attttctttt atcatagcat aaacgtcaag cgaaccagat
    12721 accagcaaat agagtggatt tctttttgtc ccttgcttta ttttccttct tttgagacct
    12781 taagtaattg attcctatca attttttaaa tatttacttt ttgaataatc aaaaacaaac
    12841 cttcactcgc aagccgagag taaggtttgt ttttcgcatt gcttatttcg tgttgtctat
    12901 tgaaattttt ccgcacctag cttttgatat agtggtgcta gttcttgtaa ggtttcagcg
    12961 gttatttgct caacgtgttc gatttcttgc ggatagaaga atagtttacc catttgaaag
    13021 gttcggtctc tcatcgcttt tttcaactct tcttgtttct ttgtatcgga caaatagtca
    13081 gacaaagcta agtggtcgtc aagttcatgt tctggttgcg gccatatgta ataatccttg
    13141 atatcgactg ttttgctcca ctctttcagt atcgcaagca actggttata cgctgcaatg
    13201 ctaccatatc gttcttcgct tttataatgt tggtacatca aatagatttg gtattgtttt
    13261 tgattcaaca aaactgctag acaggcattc tcgtcttgac gagattcgct tcggtacgcg
    13321 ctccaaaaat gactgcgtaa attccagcca ttcgtccagc tttcgatttt aggtttgctc
    13381 atgccgtaca tatcaggcaa aagtgcagct gtctggagct gaatctcttt ccatttttgc
    13441 cagtgttctt tatattcttt cttgatttgt tcaatcttat ctgttggatg gctctctttg
    13501 agctgtttga atgcgtagat tggtcgatca aacaactgat aatctgattt tccaagttcc
    13561 attgtttcgc ctctttccgt tatcttttgt attctacctg aattttgcga tatggacaaa
    13621 tagaggttgt gaaaaagctg ttctgcatca agtcataaga acagccaaac aaaaaactgc
    13681 gatgagcatc accgcagttt tattgaactt aaggttgatt gatcacatct aaaagcgttg
    13741 aaaataaaat ggcactagaa gcagctcctg ggtcgatatg tccgattgca cgctctccta
    13801 gataggaagc tcgtcctttt ttagctacca agactttggt gtgttctttt gcctggtcaa
    13861 tgatttcttt tgttaaattc cctgccttca atgcctctgt gactggcagc caaacatcta
    13921 acatcgtttt atcgtcagct tctgcctttc ctcggctttg aatacccgct gttccctgct
    13981 cgatcacttc gcctaatttt tcttgagaat caatagaatc gacctctttt gtcgctttca
    14041 tcatgttcat gaatgcagat ccgtataacg gaccagaagc cccaccgact ttcgatatca
    14101 tcgccataga gaatactttg aatgtatcgc tgattgtttc aggatgatcg gcttgaaaag
    14161 cttcttcata agctgaaacc cctcttccca tgttatttcc atggtctcca tcgccaatcg
    14221 gggtatctag atcactgaga tattgtttgt tctgattgat tgcctctgca aattgcgtga
    14281 gccattcttg gatttcttga acggttagtt tcatattatc acccttttat cttgagattt
    14341 tttaccatga aatcgtctgt acattgcttt ctaaagaatc tttccattgt gtctccgtca
    14401 agtcgatcag tgttagagaa agaccttgca tatctagtga ggtcataaaa ttaccgactt
    14461 ttcggaaaac tacattgacg cctctttctt ccaacagagc taaaacatca ttcatgaaca
    14521 cgaactgttc catcaaaggc gtcccgccca ttccgttgac tagtacacct gcttcttttg
    14581 gtaaattcga gtaatcatct aaaatcttag taacaagttc ctttgccagt actttagaag
    14641 gctgcatctt ttctcgacga tagcctggct caccgtggat accaacacca tactcgatct
    14701 catcttctcc tagttcgaat cctggtttcc ctacttctgg tacagttgct gctcgaagcg
    14761 ctagaccgat cgtcttagtt gcctgtacga ccttttctcc cagttctttc aactcagcta
    14821 gggaagcacc gtttcttgct gcatcgccca cgattttgtg tacaaggaca gtacctgcaa
    14881 ctccgcgttt tccagcagta tacgtactat tttcaacggc gatatcatca tctacgatca
    14941 cactttctac ttcaatatcg tccatagcag ctaattcctt tgccatatcg aagttcaatg
    15001 catcccctgt ataattctta acgatcagca aaactccttt tccactatcg gcttccttaa
    15061 tagccgtctg aatctgatct ggagttggag aagtaaacac atcaccaagc accgctgcac
    15121 ttaacatccc ttcccctaca aacccggcat gggatggttc atgtccgctt cctccaccag
    15181 aaaccaaacc aacttgttcc gctttctgat tcttggctac aacacgggaa gattctacac
    15241 gatgaacaag ttctggatag ctttttacta atccttccag catttcttcg acaatattgc
    15301 ttggatcatt catgattttt ttcaatttat tcccatcctt tctttccggc attttgtgct
    15361 tctgttaaga tttgctgagt atcatctgtt ataccggcag tgatggctgc tgcaaaagct
    15421 ccttccacta aaggcgcatc caccagatga taacgttgct gctgctcttc ttccaacatg
    15481 tcttttgcca gttccgagtt caataccgcg ctaccgatat ccgcaaaaac aaaataacta
    15541 gctgcatcgc ttgattcatt gactgcttct acgattttca tcgggtctga tccgatctcg
    15601 ccatttattc cgccgagaga aaaaatttgc acctcttcgg atttttgcat ctgctcaatc
    15661 atttctttaa taccatctgt tactttttga ctatgagaaa taagcaaaat actttttttc
    15721 acaagaatct tcctttcttt tacatagttg ctaacgattg gtttcgcttt cattaatgaa
    15781 caattcaagt atataataaa tttctaattt tgggaaaata tgtgtagtgc ttcttgaagt
    15841 ttgttatact ttaattaaaa gagaggagtg atcgaatgat tacaacaaca acgaacagtg
    15901 tagagggaaa agccgtagta gcttataaag ggatcgtttt tggtgaggtc attactggga
    15961 tccatgcatt taaagacttg gaggcggggt tgcgaaatat ctttggcggc cgctcaaaaa
    16021 gctatgaaaa cgaactaatg ggtgcaagag aagaagcact tgccgaaatg gccgaacgtg
    16081 caaaagcatt aggagctgat gcgatcattg gcgtaaaaat ggactatgaa gtattaggca
    16141 gcgacaatgg catgctgatg gtcacatgta gtggaacagc tgtctccacg agataagtag
    16201 gggacatctg ttttaataac taaagagaga tgggacatgt agtggcactc aagaattaga
    16261 tttttcagtc taacttcgag gtactctacc ttcccatcct ctctttttta tttctttttg
    16321 cggatgattc cgtaagctgc tgcgactaac acacccgccc ctgctaagga ataactgaca
    16381 gaagctgttt tttcatttgt ttttggcaaa gtctgacttg ttgatttgga tggctctgtc
    16441 gtttgctgaa ctgtttctgt cgtgcttcca gaggtcactg atttttctcc tgccgaatgc
    16501 aaaatatctt ttattcgttg gaaactagct gctaagtcag ctttttttgc ttcaaaatca
    16561 ttagcaactg tttgtgcttc gctgatcttt ccgtggattg cttgactggc cgctgtgata
    16621 tccgcttgag tttgacttag ctttccagaa gctgtttgag caacctgatt taattgctga
    16681 ttgaattctt ttgcttgttg gatcttcccg tcaagatctg tttggacagc ttccgctttt
    16741 gctttcgctt cggcaagctt tttctcaagt tgtgcacggt catttcccac tttattttta
    16801 agatcagcta aatcttgatc gattgctgct tggtcttctt tcaatttgtt ttttgcagct
    16861 tctaatgctt cttcgagttc ttttctttta ttttccaagt cttgcaaatg atcgatttgg
    16921 tcagaagagg tagaactatt tattgatgta tcttctgaag atgaaatcgt ttctcctgaa
    16981 ttatctgtcg gtacattgga agtcatttct gtttccgagg caccgttttc agcaccagaa
    17041 gagcttatgg ttgttgaagg ttctgtttga gcaggttcag aagtttccgc aaaagctgtc
    17101 tgtcccaata agaaaatact ggaaacagca attggccaga ttaacggctt cattcttttt
    17161 ttcatcactt ttcccatcct tactactttt taactaattt cttattataa gcaaaaatgt
    17221 ttggaagaac cataaacaca acaggctgtt tgtctaaaaa acggtgcatc ttttattttt
    17281 ttgtacgttc cgggcttttc catgtcccgc ttccataacc taaaatcgtt ttgactgctg
    17341 gaatgaaagt cgtcacgatc gttatcgcgt tcactatcca gaacaaaagc atatatagtg
    17401 gtgcgaaaag aaggtatttc aattttcttc gtcgatcatc tacaatcagc gaagctagca
    17461 actggaaaaa tcctgcgacc atttcaaagc agatgaacac aaaactgaat gtaaaagtaa
    17521 tataaatctg ttcgtagttt ccttgatata cataatgccc gattaatcca gcaaaaagaa
    17581 ctacggaaat acaaaagaat attgaccaga caatactcaa tgtttgatca atgaagatga
    17641 tcgttcttcc aatatgttta aaaggatgga gcatcacttt tttgaaattc gtcagccaga
    17701 cttctgttcc accttttgcc caacgttttc tgtgtcgata tagcatcttc aacgtcacag
    17761 gtacttccat gaaaaagata atctcaggag caaaaacaga taaccagtca tttaactgat
    17821 gatcccaagc aatactgatg tcttctgtcg ctcgatcttg tctgaatccg cccacatcga
    17881 tcaaggcttc ttttcgatac atcgtatttg ctccactgta cgcataaaga ccaccgaaaa
    17941 cagctgactg tgttcgttta atgatgccca cgatacttga gaattcaacc gtttgagatt
    18001 ttgcaatcaa tttggtacga ttttggacat ccatatttgc agtgaccgca gcaatatgtc
    18061 ttgcaccagg gcgaatgaaa taattcacat acctaatcag tgcatctggc tccggtaccg
    18121 tgtctgcatc attgcttaaa atcaatttcc cttttgcaaa ggcaagtccg atattaaacg
    18181 cgtgggcttt tcctttattt ttttcgatcc gaaccacacg aagattagcg tattttttca
    18241 tcagtctagc taatatttct ggtgtttggt ccgttgaacc atcgtctgtc actaatacct
    18301 catagttgtg ataattcaat ttcgtcatca aatactcgat tgtatattcg atcacaattt
    18361 cttcattatg ggctggcacc atgatcgtga tgaacggttc gacactcaag ggaacatcta
    18421 cccattcttt ttgcttatgt ttgtataaaa acacataaca ccagacacca ataaaccata
    18481 caaatccgcc taaaatcgga taagaaacga gaaaaaaata cataatagac aaacaagtat
    18541 ctaagaataa attacctgta tgggtgattg agatcatttc agttccaccc cattttcttt
    18601 atacaaatct gcaatgaatg tcgtatctag atttttctct tcggagacag aataaaaacg
    18661 gaccgtttct ctctcttctt ttgttccaaa acgttccgta tacacttctt caagtaattc
    18721 ttgtcttttt ttcaattttt catcttcata ttcaaaagat ttatttactt ttttgtatag
    18781 atttcgattg ttccacctag tcaatacaaa tgaggtaatc aggaaactga aaaaaatcaa
    18841 tacaaaaaag ccgattaaga aatacagcat tttcttttct gataaaaaag cgtacaagga
    18901 aaaatccttg gctctttctg gtctcgttaa tgggaaaaag acccatataa aaggaagaac
    18961 aacgatcatc catcctaaaa gagccactaa cgtttgccaa atctttaacc agtaattacc
    19021 ttttgaaaaa tacttatctt tcaccaaaat tgatgcaact tcttctcttg ttactttttt
    19081 catatcctca cctcacgttt ttttgttacg acgtaggagt ttaccataca tttcatttct
    19141 tttcacataa agtgttccta aatttttcct aagaaaaaga acaaaacatt tctcatcagc
    19201 aaatgaaaaa tattttgttc tttttatcag ttgttctttt tattttttat ctcttaatta
    19261 tgttgctctt tccaatacga caatgctttt cattcctcta gatatcgccg tgtacatttg
    19321 tctgcgtctt ctttgctcat ctccatcgtt ttcaaatgga tataagatta catgatcaaa
    19381 ttccagtcct tttgctagac tcaaaggaat gatttggcag ttttctaatc catatgaatt
    19441 ttttttaatc gattcttgta gcgtttgggt ttcctcttct gtttgggtga taactgccgc
    19501 tttttctcct tttagagaaa ccaaaatctc agctagtatt ttcaacaatt cttcagagga
    19561 tcgaaaagag cggtattcgg gttcttttcc ttgcggtcgg atcgatacga tgtcgatctt
    19621 ctcttgatcc acagcatagg ttttgaaaag ctctgtgatt gctccgctag aacggtaact
    19681 atttctaaga tcataccgat ggatcggcag atggtagtcg tcgaaacaac gcataatatc
    19741 agcaaaagta gtggaagaat taaagattgc ctgattctcg tcacctacta aagtaaagtg
    19801 agtttttgga aataattcga tcaatagacc taattgtgcc agggtataat cttgtacttc
    19861 atcaatcaat agatagcgaa atgctggtac atttaccttt tcaacaagta aatgacgaat
    19921 agctaatata atgactgctt catcaacagt acgtgtgggt tgccaagcat aagctgagcc
    19981 atatcgtttt tcataaattc tttcaaacaa ctgatgctca tccacccatg ccatttcttc
    20041 gatttgtctg gttatttttc gatatttttt ttgtaaaagt ttccttgcat aagctacaat
    20101 actttgttcc gaatcatcag aaatcaattt accgaataat tccaactgcc tatcttctgt
    20161 caacgagagt acctgattct gcacagcagg actttttgct tgtttcaaaa gatgattctt
    20221 ccattcgctc atcagtgcct ttttcgttgc ttgaagccta tcgatcaatt taggatgagc
    20281 aggaactttc tcatatattt tttcgatcgt ttttttagaa aggactgttt ttccattttt
    20341 agtaagtcca cggataaaat tcggatgatc taaaaacaaa ggatctgatt gttttagatt
    20401 atcgataaac tttttgctac gcaaacgttc tgtctgctcc gatacatttt ctcccgtgat
    20461 acgtttgaaa taagcctctt caccttctat ttcctctgct gaaagctgtg aaaccagctg
    20521 tatgatggtt agattcagtg gatttctttc acctaatgat ggtaggacat tcgaaatata
    20581 gtcgataaac cgatgattag gtgatagaat cagcaagtca tcggatgtca tcgtatctcg
    20641 atataaatag agcagatacg caatacgctg catgatagca gaggttttac cacttcctgc
    20701 tacgccattt accaagatat tttgactttt cacatcccga atgattgtgt tttgttccga
    20761 ttggatcgta gcagtgatag ccttcatttc attggtctca tcttggccta agacttctaa
    20821 taatacgtca tcttgaatag ctacagacgt atcaaaaaac ttgaccaaac gatctctttc
    20881 caaaagaaat tgtcgtcttt ctttaataac cgtatcgatt ttattttgac gaaccgtata
    20941 agaagatgga ccaatcatgt tattgtaaaa aagttcggca attggtgaac gccaatcata
    21001 aacaagattt tcatctgcat catctgtgaa atttgctgta ccgatataaa aggattcttt
    21061 tggctcatta tccagaaagt ctacaacgac ttttccaaag tagggacttt tcaataaacg
    21121 ttcgatcttt gctagctgat tttctgcgtt tttctgtttg atattcatct gatcgatttc
    21181 tctatttttc atttcaatca tcgaaaaagt atccaggtta tctaagtaac tgtcaaaatt
    21241 caatcgaagc tcgccagcca tttctttcaa cgactggttt ccttctattt ttgccgtatc
    21301 catcgattcc cgcagttcgt tttttttatc aattagcttt tgatacacgt tcttcagata
    21361 tgcttcttct aactgttttt gtgtgttgac catacacatt ctcctctttt tttaaagtgg
    21421 tggaaccgat tagtttacct cttttaagag tcgaatgcaa gctttttaac gagtatcatt
    21481 tgttaggata tatcagttct tcaggtcgga cagtttgata taaagaaaat gcaccatcaa
    21541 ggttttttac gtggtagcca gactgcttca agattctttc cgctagatag ctacgtaatc
    21601 cgctatgaca gctgacgata tattcttgtt ggctgtctaa ttcatttaat cggtcccgaa
    21661 gttcattcaa agggatatgt ttggcttcct tgaatttgcc cttttccaat tcttctgctg
    21721 ttcgcacatc taataaaatt tttccttttg ctagttctgt cggcaattca tgccattgta
    21781 cggtttcact aagcccttct gcaatgttca ttgctgcata tcctagcatg tttactggat
    21841 ctttggctga gccaaatggc ggtgcatacg tcaattctaa ttccggtaaa tcgaagatcg
    21901 tcaaatgccc tttgattgct gttgctaaaa tatcgatccg tttatccaca ccttttgccc
    21961 caacaccttg tgcaccataa atctcgccag tctctggatg gaagatcaac ttcagtaaaa
    22021 tatctgttgc ttctggataa tagcttgcat gatcttttcc tgatacatga atgacttcaa
    22081 aagacagttc gttttctttg gctgttcttt cacttaaacc ggtggatgcg gcagtcattc
    22141 caaacgttcg tataattgct gttccaatac cgccttgatt tcttcgagaa attccggaaa
    22201 tcgtgtccgc aacttgtcta ccttgacgat tggctggtga agcaagagaa ataagtgcat
    22261 cttttccagt gatttgctgt tttacaacga tagcatcccc caccgcataa atatcaggaa
    22321 tattcgtttg ataacgttcg tctactagga tacccccgcg tatgcctaat tcgattccag
    22381 cggtcactgc tagctggtta tcaggacgaa caccaatgga taaaataact aaatcagcag
    22441 ggatggtacg cccatcatcc agtcgcaatc gtttcccgtg gtcttcaaat ccagcgacag
    22501 catgagaagt aatcacttct actccttgat gagacaattc tttttctata aaagctgcca
    22561 tttcttcatc cagtgttgga agaatatgtg gtgcttgttc gatcaccatg acttctaaac
    22621 ctcttctttt taggttttct gccatttcca atccgatgaa tcctgcacca acgatcacgg
    22681 cacgctttgg ctgtttttct aatgcatgca tcactttatc gatatctggt acatttctaa
    22741 tagaaaaaac attgtcggct tctgttatcc ctggaatcga tggaacaact ggttttgcac
    22801 caggagataa aaccaatgca tcgtactgtt caatgtgttt ttgaccctca tgtacgactt
    22861 ctaccgtttt gttttcggga aagatggctg tcacttcatg atttggacgc acatctaaac
    22921 aaaaacgagc ggataatgat tctggcgttt ggacaagaag attttcccgt tcactgattt
    22981 ctcccgaaag ataataaggc aagccgcaat ttgcaaaaga aacatacggt cctttttcaa
    23041 aaacaacgat ttctgcatct tccattaatc ggcgaagccg tgtagcagca gacattccac
    23101 ctgctacacc tccgacaata acgattttca tcttatttcc ctcctactgt tcgatcaaac
    23161 cattgattca tgccaccacg tacattcact acatcataac cagatttctt taattcttta
    23221 gctgcttgtt tactcctcat ccctgattga caaatcacat agacggtctt ttctttgtct
    23281 ccatcatatc tgtttatact ttgtaatgga acatttttag ctccttttat atgcccgccc
    23341 cggtattctg caggtgttct aacatcaagt aacgctggat tttccaaaag cttttccttt
    23401 aattctggga ctgttatcga ttttattgat tgaaatagca aattcatttt ctgcacctca
    23461 tttacatttt tataaatacc tcatggggta tattaaacag gagctttatt aatatgtcaa
    23521 ccagaatgac tcttcatttt tttcactact ttttatattt gcataaaata ccgataaggg
    23581 gtattatcta attataaaaa tagaggagaa tgtgttatgg agattgataa taaaaatatc
    23641 ctgaaccgtt tgaaacgtac agagggacaa attcgtggta tccaaaaaat gatcgaagac
    23701 gaaaaagaat gtatggatgt catcactcaa ttaagcgctg ttcgttctag tattgatcgc
    23761 gtaatgggca tgatcgttgc agataatctg aaaaattgct ttgagaaacc tgatagtaat
    23821 ccagaagaac aagcggctaa actagaacaa gcgatcaaga tgattattaa aaagtaaagg
    23881 aatgatttca atgaaagtcg ttttccatat tgatgaatta gaaaaatggt cagaaacagg
    23941 gaaaaacgta aaaaacctga taaaagcttc tccagaaacg acgattgtcg tatctgtaaa
    24001 tggcatcgcg attaccggtt atctagactc tgccaatgca gaattcttag atttgcaagg
    24061 agtcgttttt cacgcttgcg ccaatgctat gcgtgctaat catatttcag aaagttcttt
    24121 acctgaacaa gtaatcgttg taccagcagg tgtccttgat ttagttgaac tgcaatccca
    24181 aggttatgct tatatcaagc cttagtcagt agaggttgcg acagaagtga tcagcttcaa
    24241 gaaatttcgg cttatttttc gaagaagcta cttctggaaa accgtgaatc aaaggttgtg
    24301 aaaa
//
LOCUS       DBNIBO010000027        24271 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.027, whole genome shotgun sequence.
ACCESSION   DBNIBO010000027 DBNIBO010000000
VERSION     DBNIBO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.027"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..572
                     /locus_tag="AC1Y77_000932"
     CDS             <1..572
                     /locus_tag="AC1Y77_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIQ1574528.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="AC1Y77_000933"
     CDS             576..1217
                     /locus_tag="AC1Y77_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574529.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="AC1Y77_000934"
     CDS             1214..2131
                     /locus_tag="AC1Y77_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1574530.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="AC1Y77_000935"
     CDS             2128..2799
                     /locus_tag="AC1Y77_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574531.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="AC1Y77_000936"
     CDS             complement(3340..3828)
                     /locus_tag="AC1Y77_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HIQ1574532.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="AC1Y77_000937"
     CDS             complement(4029..4295)
                     /locus_tag="AC1Y77_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIQ1574533.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="AC1Y77_000938"
     CDS             complement(4295..4558)
                     /locus_tag="AC1Y77_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIQ1574534.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="AC1Y77_000939"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="AC1Y77_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="AC1Y77_000940"
     CDS             complement(5361..7184)
                     /locus_tag="AC1Y77_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1574535.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="AC1Y77_000941"
     CDS             complement(7782..10487)
                     /locus_tag="AC1Y77_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HIQ1574536.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="AC1Y77_000942"
     CDS             complement(10793..11740)
                     /locus_tag="AC1Y77_000942"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIQ1574537.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="AC1Y77_000943"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="AC1Y77_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIQ1574538.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="AC1Y77_000944"
     CDS             complement(12375..12737)
                     /locus_tag="AC1Y77_000944"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIQ1574539.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="AC1Y77_000945"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="AC1Y77_000945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIQ1574540.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="AC1Y77_000946"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="AC1Y77_000946"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIQ1574541.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /gene="cshA"
                     /locus_tag="AC1Y77_000947"
     CDS             complement(14365..15876)
                     /gene="cshA"
                     /locus_tag="AC1Y77_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="degradosome RNA helicase CshA"
                     /protein_id="HIQ1574542.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="AC1Y77_000948"
     CDS             complement(16198..17550)
                     /locus_tag="AC1Y77_000948"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIQ1574543.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="AC1Y77_000949"
     CDS             complement(17703..18779)
                     /locus_tag="AC1Y77_000949"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIQ1574544.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNVLATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="AC1Y77_000950"
     CDS             18975..20720
                     /locus_tag="AC1Y77_000950"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIQ1574545.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="AC1Y77_000951"
     CDS             complement(21000..21437)
                     /locus_tag="AC1Y77_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIQ1574546.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="AC1Y77_000952"
     CDS             complement(21421..22794)
                     /locus_tag="AC1Y77_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574547.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="AC1Y77_000953"
     CDS             complement(23116..23604)
                     /locus_tag="AC1Y77_000953"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1574548.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="AC1Y77_000954"
     CDS             complement(23626..24228)
                     /locus_tag="AC1Y77_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIQ1574549.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6961 a         5163 c         4486 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcat aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaaaca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DBNIBO010000028        23827 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.028, whole genome shotgun sequence.
ACCESSION   DBNIBO010000028 DBNIBO010000000
VERSION     DBNIBO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.028"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(53..787)
                     /locus_tag="AC1Y77_000955"
     CDS             complement(53..787)
                     /locus_tag="AC1Y77_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIQ1574550.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="AC1Y77_000956"
     CDS             1114..1524
                     /locus_tag="AC1Y77_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIQ1574551.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="AC1Y77_000957"
     CDS             1517..2218
                     /locus_tag="AC1Y77_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIQ1574552.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="AC1Y77_000958"
     CDS             2218..3804
                     /locus_tag="AC1Y77_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574553.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="AC1Y77_000959"
     CDS             3823..4044
                     /locus_tag="AC1Y77_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HIQ1574554.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="AC1Y77_000960"
     CDS             4041..4802
                     /locus_tag="AC1Y77_000960"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIQ1574555.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="AC1Y77_000961"
     CDS             5182..5691
                     /locus_tag="AC1Y77_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIQ1574556.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="AC1Y77_000962"
     CDS             complement(5766..6305)
                     /locus_tag="AC1Y77_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIQ1574557.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="AC1Y77_000963"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="AC1Y77_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIQ1574558.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="AC1Y77_000964"
     CDS             7318..8169
                     /locus_tag="AC1Y77_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIQ1574559.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="AC1Y77_000965"
     CDS             8197..8832
                     /locus_tag="AC1Y77_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIQ1574560.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="AC1Y77_000966"
     CDS             8852..9625
                     /locus_tag="AC1Y77_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1574561.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="AC1Y77_000967"
     CDS             10033..10830
                     /locus_tag="AC1Y77_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIQ1574562.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="AC1Y77_000968"
     CDS             10808..11221
                     /locus_tag="AC1Y77_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HIQ1574563.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="AC1Y77_000969"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="AC1Y77_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIQ1574564.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="AC1Y77_000970"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="AC1Y77_000970"
                     /inference="COORDINATES: protein motif:HMM:NF000950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIQ1574565.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="AC1Y77_000971"
     CDS             15998..16558
                     /locus_tag="AC1Y77_000971"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIQ1574566.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="AC1Y77_000972"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="AC1Y77_000972"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HIQ1574567.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="AC1Y77_000973"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="AC1Y77_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HIQ1574568.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="AC1Y77_000974"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="AC1Y77_000974"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIQ1574569.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="AC1Y77_000975"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="AC1Y77_000975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIQ1574570.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="AC1Y77_000976"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="AC1Y77_000976"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIQ1574571.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="AC1Y77_000977"
     CDS             21905..22483
                     /locus_tag="AC1Y77_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1574572.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="AC1Y77_000978"
     CDS             22535..23017
                     /locus_tag="AC1Y77_000978"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIQ1574573.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="AC1Y77_000979"
     CDS             23134..23508
                     /locus_tag="AC1Y77_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574574.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atagaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DBNIBO010000029        23646 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.029, whole genome shotgun sequence.
ACCESSION   DBNIBO010000029 DBNIBO010000000
VERSION     DBNIBO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.029"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            1..168
                     /locus_tag="AC1Y77_000980"
                     /pseudo
     CDS             1..168
                     /locus_tag="AC1Y77_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            346..642
                     /locus_tag="AC1Y77_000981"
     CDS             346..642
                     /locus_tag="AC1Y77_000981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574575.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="AC1Y77_000982"
     CDS             649..2601
                     /locus_tag="AC1Y77_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1574576.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="AC1Y77_000983"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="AC1Y77_000983"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HIQ1574577.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3445..4572
                     /locus_tag="AC1Y77_000984"
                     /pseudo
     CDS             3445..4572
                     /locus_tag="AC1Y77_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            4673..4858
                     /locus_tag="AC1Y77_000985"
     CDS             4673..4858
                     /locus_tag="AC1Y77_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIQ1574578.1"
                     /translation="MNYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINVQ
                     KMNIITIKEPDAKTQSR"
     gene            complement(4916..5269)
                     /locus_tag="AC1Y77_000986"
     CDS             complement(4916..5269)
                     /locus_tag="AC1Y77_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574579.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5800..6222
                     /locus_tag="AC1Y77_000987"
     CDS             5800..6222
                     /locus_tag="AC1Y77_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIQ1574580.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6219..6449
                     /locus_tag="AC1Y77_000988"
     CDS             6219..6449
                     /locus_tag="AC1Y77_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574581.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6947..7147
                     /locus_tag="AC1Y77_000989"
     CDS             6947..7147
                     /locus_tag="AC1Y77_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HIQ1574582.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7174..8367
                     /locus_tag="AC1Y77_000990"
     CDS             7174..8367
                     /locus_tag="AC1Y77_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1574583.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="AC1Y77_000991"
     CDS             complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="AC1Y77_000991"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIQ1574584.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10059..10982)
                     /gene="coaA"
                     /locus_tag="AC1Y77_000992"
     CDS             complement(10059..10982)
                     /gene="coaA"
                     /locus_tag="AC1Y77_000992"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIQ1574585.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11178..11783
                     /locus_tag="AC1Y77_000993"
     CDS             11178..11783
                     /locus_tag="AC1Y77_000993"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1574586.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12091..13107
                     /locus_tag="AC1Y77_000994"
     CDS             12091..13107
                     /locus_tag="AC1Y77_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574587.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13248..14549
                     /locus_tag="AC1Y77_000995"
     CDS             13248..14549
                     /locus_tag="AC1Y77_000995"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIQ1574588.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14595..15257
                     /gene="deoC"
                     /locus_tag="AC1Y77_000996"
     CDS             14595..15257
                     /gene="deoC"
                     /locus_tag="AC1Y77_000996"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIQ1574589.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15278..15676
                     /locus_tag="AC1Y77_000997"
     CDS             15278..15676
                     /locus_tag="AC1Y77_000997"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIQ1574590.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15742..16824
                     /locus_tag="AC1Y77_000998"
     CDS             15742..16824
                     /locus_tag="AC1Y77_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIQ1574591.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17036..18598
                     /locus_tag="AC1Y77_000999"
     CDS             17036..18598
                     /locus_tag="AC1Y77_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574592.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18591..19724
                     /locus_tag="AC1Y77_001000"
     CDS             18591..19724
                     /locus_tag="AC1Y77_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574593.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19724..20680
                     /locus_tag="AC1Y77_001001"
     CDS             19724..20680
                     /locus_tag="AC1Y77_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574594.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20779..21945
                     /gene="deoB"
                     /locus_tag="AC1Y77_001002"
     CDS             20779..21945
                     /gene="deoB"
                     /locus_tag="AC1Y77_001002"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIQ1574595.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21969..22787
                     /locus_tag="AC1Y77_001003"
     CDS             21969..22787
                     /locus_tag="AC1Y77_001003"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1574596.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22804..23511
                     /gene="deoD"
                     /locus_tag="AC1Y77_001004"
     CDS             22804..23511
                     /gene="deoD"
                     /locus_tag="AC1Y77_001004"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1574597.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7688 a         4188 c         4933 g         6837 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
     3841 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
     3901 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
     3961 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
     4021 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
     4081 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
     4141 tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt
     4201 cggatgctta ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta
     4261 gagccatatc ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac
     4321 gatgctccca aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt
     4381 attattggag aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt
     4441 acaaatgggc ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa
     4501 cctgtttgcc agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat
     4561 aaaataactt agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga
     4621 aatattcttt aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta
     4681 caagtggata ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac
     4741 tatacttaaa aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt
     4801 tcaaaaaatg aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt
     4861 tgagaaatga aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc
     4921 cttcaatatt tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt
     4981 tgtctgtaaa gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat
     5041 tatctgtagg taagaaaaat tcatcaatag atacatggag taatgataca agatcataca
     5101 gcacttgtgt acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa
     5161 tttcaatcat tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa
     5221 tcgctaaacc aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc
     5281 tctatacatt ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag
     5341 tttatgagtt cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata
     5401 gaaaggtcgc tgattttatt tacctataca actttataaa atttagtcat actcctagaa
     5461 agtagataaa attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca
     5521 tctagttctc ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat
     5581 gaccatcaat gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga
     5641 aaacaatctg tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc
     5701 atttatgcaa caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca
     5761 catgccactc cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa
     5821 gaaaccatag aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa
     5881 gattacttca aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat
     5941 aactaccttg tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt
     6001 aaattatttg gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat
     6061 ctttcagaga aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg
     6121 gagattgcgg agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga
     6181 ctagccatga ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg
     6241 ttcccttccc cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg
     6301 ttaaacatta tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg
     6361 gaaattctca tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct
     6421 ctaagatttt atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg
     6481 tatccacgct tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt
     6541 aggttttgat aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata
     6601 cgtttcggta agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg
     6661 attcatgtta gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg
     6721 tgataatgat actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt
     6781 acggctacat gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga
     6841 ttgctaagat attcttctct tgtcagtgta ttattataaa actatttgat ataattatag
     6901 gtgaatattg gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac
     6961 aaaaaaggat ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa
     7021 agcaaaagaa ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag
     7081 agtccctcgt gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc
     7141 tgagtagttt ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca
     7201 aagaatggaa cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt
     7261 caaaaatatc gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg
     7321 ctattgatga aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat
     7381 atctttgaaa ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat
     7441 cgcttaccaa ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt
     7501 gagcctattg atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat
     7561 gtaagagctg ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata
     7621 accaccacaa atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa
     7681 ttttggacaa aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat
     7741 caacactttt tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa
     7801 gctactgcca ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa
     7861 acactctatt acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc
     7921 gttcgtcata ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta
     7981 caacaaaatc taattcagac agactttgta atgagctata acggtattcc tacccaaaaa
     8041 catacactat catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga
     8101 atccatgggt tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt
     8161 atcattaaag atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat
     8221 ctttatccta acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa
     8281 gaagcaaaac aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga
     8341 aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac
     8401 ccttggtata agaggatttc tagctctcct acaactattc ccactccact gttgctggtg
     8461 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
     8521 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
     8581 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
     8641 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
     8701 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
     8761 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
     8821 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
     8881 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
     8941 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
     9001 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
     9061 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
     9121 acttgtctaa gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc
     9181 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
     9241 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
     9301 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
     9361 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
     9421 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
     9481 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
     9541 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
     9601 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
     9661 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
     9721 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
     9781 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
     9841 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
     9901 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
     9961 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    10021 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    10081 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    10141 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    10201 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    10261 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    10321 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    10381 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    10441 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    10501 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    10561 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    10621 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    10681 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    10741 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    10801 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    10861 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    10921 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    10981 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    11041 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    11101 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    11161 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    11221 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    11281 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    11341 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    11401 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    11461 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    11521 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    11581 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    11641 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    11701 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    11761 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    11821 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    11881 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    11941 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    12001 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    12061 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    12121 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    12181 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    12241 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    12301 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    12361 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    12421 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    12481 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    12541 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    12601 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    12661 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    12721 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    12781 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    12841 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    12901 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    12961 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    13021 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    13081 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    13141 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    13201 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    13261 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    13321 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    13381 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    13441 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    13501 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    13561 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    13621 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    13681 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    13741 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    13801 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    13861 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    13921 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    13981 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    14041 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    14101 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    14161 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    14221 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    14281 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    14341 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    14401 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    14461 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    14521 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    14581 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    14641 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    14701 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    14761 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    14821 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    14881 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    14941 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    15001 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    15061 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    15121 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    15181 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    15241 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    15301 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    15361 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    15421 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    15481 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    15541 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    15601 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    15661 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    15721 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    15781 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    15841 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    15901 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    15961 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    16021 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    16081 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    16141 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    16201 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    16261 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    16321 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    16381 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    16441 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    16501 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    16561 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    16621 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    16681 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    16741 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    16801 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    16861 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    16921 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    16981 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    17041 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    17101 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    17161 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    17221 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    17281 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    17341 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    17401 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    17461 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    17521 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    17581 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    17641 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    17701 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    17761 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    17821 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    17881 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    17941 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    18001 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    18061 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    18121 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    18181 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    18241 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    18301 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    18361 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    18421 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    18481 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    18541 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    18601 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    18661 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    18721 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    18781 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    18841 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    18901 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    18961 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    19021 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    19081 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    19141 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    19201 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    19261 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    19321 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    19381 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    19441 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    19501 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    19561 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    19621 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    19681 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    19741 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    19801 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    19861 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    19921 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    19981 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    20041 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    20101 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    20161 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    20221 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    20281 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    20341 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    20401 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    20461 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    20521 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    20581 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    20641 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    20701 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    20761 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    20821 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    20881 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    20941 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    21001 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    21061 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    21121 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    21181 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    21241 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    21301 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    21361 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    21421 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    21481 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    21541 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    21601 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    21661 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    21721 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    21781 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    21841 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    21901 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    21961 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    22021 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    22081 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    22141 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    22201 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    22261 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    22321 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    22381 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    22441 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    22501 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    22561 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    22621 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    22681 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    22741 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    22801 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    22861 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    22921 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    22981 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    23041 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    23101 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    23161 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    23221 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    23281 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    23341 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    23401 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    23461 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    23521 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    23581 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    23641 tgtgaa
//
LOCUS       DBNIBO010000030        20903 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.032, whole genome shotgun sequence.
ACCESSION   DBNIBO010000030 DBNIBO010000000
VERSION     DBNIBO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.032"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(93..536)
                     /locus_tag="AC1Y77_001005"
     CDS             complement(93..536)
                     /locus_tag="AC1Y77_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HIQ1574598.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(754..2094)
                     /locus_tag="AC1Y77_001006"
     CDS             complement(754..2094)
                     /locus_tag="AC1Y77_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1574599.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2363..3259
                     /locus_tag="AC1Y77_001007"
     CDS             2363..3259
                     /locus_tag="AC1Y77_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HIQ1574600.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3409..4350)
                     /locus_tag="AC1Y77_001008"
     CDS             complement(3409..4350)
                     /locus_tag="AC1Y77_001008"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIQ1574601.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4476..5597
                     /locus_tag="AC1Y77_001009"
     CDS             4476..5597
                     /locus_tag="AC1Y77_001009"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIQ1574602.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5855..7357
                     /gene="eat(A)"
                     /locus_tag="AC1Y77_001010"
     CDS             5855..7357
                     /gene="eat(A)"
                     /locus_tag="AC1Y77_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HIQ1574603.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7424..7951
                     /locus_tag="AC1Y77_001011"
     CDS             7424..7951
                     /locus_tag="AC1Y77_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1574604.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8162..9223
                     /locus_tag="AC1Y77_001012"
     CDS             8162..9223
                     /locus_tag="AC1Y77_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIQ1574605.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9226..10122
                     /gene="murQ"
                     /locus_tag="AC1Y77_001013"
     CDS             9226..10122
                     /gene="murQ"
                     /locus_tag="AC1Y77_001013"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIQ1574606.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10136..12118
                     /locus_tag="AC1Y77_001014"
     CDS             10136..12118
                     /locus_tag="AC1Y77_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIQ1574607.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="AC1Y77_001015"
     CDS             complement(12183..12629)
                     /gene="nrdI"
                     /locus_tag="AC1Y77_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIQ1574608.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12840..14264
                     /locus_tag="AC1Y77_001016"
     CDS             12840..14264
                     /locus_tag="AC1Y77_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HIQ1574609.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14445..15218)
                     /locus_tag="AC1Y77_001017"
     CDS             complement(14445..15218)
                     /locus_tag="AC1Y77_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1574610.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15215..16120)
                     /locus_tag="AC1Y77_001018"
     CDS             complement(15215..16120)
                     /locus_tag="AC1Y77_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574611.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16274..16594)
                     /locus_tag="AC1Y77_001019"
     CDS             complement(16274..16594)
                     /locus_tag="AC1Y77_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIQ1574612.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17068..17754
                     /locus_tag="AC1Y77_001020"
     CDS             17068..17754
                     /locus_tag="AC1Y77_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HIQ1574613.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17756..18700
                     /locus_tag="AC1Y77_001021"
     CDS             17756..18700
                     /locus_tag="AC1Y77_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HIQ1574614.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18899..19543
                     /gene="pcp"
                     /locus_tag="AC1Y77_001022"
     CDS             18899..19543
                     /gene="pcp"
                     /locus_tag="AC1Y77_001022"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIQ1574615.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19622..20899
                     /locus_tag="AC1Y77_001023"
     CDS             19622..20899
                     /locus_tag="AC1Y77_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574616.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6753 a         3767 c         4344 g         6039 t
ORIGIN      
        1 cttattcacg gcgttccaga agtagcttct tcttatttct tgaagctgat aacttctgga
       61 acatcctctt tttttatttg gacggataat ggtcattcca tttctttgat catttttttc
      121 attttgtcga tctcttcttc ttgtgcctga atgatttctt ccgctaattt ttttacttca
      181 ggatctttta aatcagctcg tttacttgcc aggatagcga tagaatgatg aggaatcatg
      241 gccttcatcc aagctgtgtc gttgacagta gtttggcttc gtaccataaa caaggaaccg
      301 gcaaaaacaa gaagactagc ggctaagatc ataacattta tctttttgtt ttcatacatt
      361 ttccacatga acaacagcat gatgactgcc atgactgcac ccatcatcaa cgccatgaat
      421 actcgtgtac gactgaagaa gatatgatcg agcgcataga cattcaagta catcatacca
      481 aacataacta ccgttgaaac accgatcatc aacaaaaact tccaatactt tttcatttaa
      541 aacaaactcc tttttttcat tcctttcttc tatcatgtca tgagcatgga gaacaatcaa
      601 ttattttgca cagagggttt ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc
      661 ggaaaaagga gttgcaacaa gacttttgtc acaactcctt tcttcaatag ctccatctat
      721 tccacataaa taaaataaaa ttttctatcg atttcattca ttcgtcaata cgaacgcatc
      781 attctttttt tgataactga aataaatgaa caggtaaagc aggccagtca atacagttac
      841 cccaatggag ttcgtgaaca ggataccaag gaaaatcaat cctaagacag ctaatgcaaa
      901 aaagctgatt cctttatccc ataatgttcc ttttcttagt gccagtacaa aggatgccgc
      961 actgataatg atccagatca ataatactgt atacgaaagg gaacctgcca gatagccaaa
     1021 taatttgtca ccgacaaaat aagaaagagc tacaccaaga taaagaacac tggtacaaaa
     1081 taaaacagca cgttgcggta cttgatgctt gtttaaaaca gctagttttc tagaggaacc
     1141 ttttcgatcc ttcaaacgga agaacaaaat tcttgaagat gcatacacac ccgaattgat
     1201 agatgagaac aaagcaagga caatcacaaa attgacgata tccgctgcaa atgggatatt
     1261 cattttttca aataccatca caaaaggact gacattcgtg cctgctaaag tattccaagg
     1321 aaaaagaatc aaaagcaaaa acaaaggaat gatgtaaaag gaaatgattc ttcccatgac
     1381 acctttgatt gctttaggaa tcgcacgttt aggatcatct gcttcgctta ccgtgatagc
     1441 aatcagttca gaaccgccgt aagaatagat aactacaagc aaagaactga tgattccctt
     1501 cataccatga ggagtaaacc caccatggtc agtcaattgc tgaagggtag ggaaaacgcc
     1561 tgtgcccaac atttgctgac cgacaagata agtagcaaaa atgatcagta agataatgac
     1621 actgatttta gcaaaagcca gccaatattc tgtttctgcg aataaccgta ccgaataaag
     1681 attgatgatc gtagttaaaa cagccaacat aaaaacaaat acccaggctg gaatgtgcgg
     1741 gaaccacaac tgcaaaaagg aagcagctgc aacagcttca gcgatgatat tgataatcca
     1801 catagaccag tagacccaat cagtaaaatc agcagaatga gcacctagat aagggcgtat
     1861 caaaccagat aatccgtgat gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt
     1921 catcacgata tataaaatca gcccaccaat caaaaaagca atcaatacag aagggccggc
     1981 gatcccaatc gcttcgccac ttcctttaaa tagacctgca ccgattgctc cacctaaagc
     2041 taacatggtg atatgtctgg agctaagttg ttttttcaat tttttatctt ccataaatga
     2101 cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca
     2161 atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat
     2221 tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa
     2281 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
     2341 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
     2401 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
     2461 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
     2521 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
     2581 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
     2641 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
     2701 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
     2761 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
     2821 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
     2881 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
     2941 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
     3001 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
     3061 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
     3121 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
     3181 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
     3241 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     3301 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     3361 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     3421 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     3481 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     3541 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     3601 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     3661 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     3721 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     3781 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     3841 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     3901 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     3961 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     4021 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     4081 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     4141 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     4201 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     4261 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     4321 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     4381 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     4441 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     4501 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     4561 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     4621 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     4681 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     4741 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     4801 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     4861 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     4921 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     4981 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     5041 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     5101 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     5161 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     5221 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     5281 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     5341 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     5401 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     5461 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     5521 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     5581 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     5641 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     5701 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     5761 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     5821 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     5881 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     5941 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     6001 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     6061 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     6121 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     6181 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     6241 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     6301 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     6361 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     6421 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     6481 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     6541 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     6601 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     6661 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     6721 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     6781 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     6841 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     6901 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     6961 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     7021 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     7081 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     7141 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     7201 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     7261 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     7321 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     7381 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     7441 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     7501 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     7561 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     7621 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     7681 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     7741 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     7801 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     7861 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     7921 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     7981 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     8041 atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg
     8101 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
     8161 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
     8221 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
     8281 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
     8341 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
     8401 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
     8461 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
     8521 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
     8581 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
     8641 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
     8701 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
     8761 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
     8821 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
     8881 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
     8941 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
     9001 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     9061 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     9121 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     9181 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     9241 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     9301 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     9361 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     9421 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     9481 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     9541 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     9601 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     9661 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     9721 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     9781 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     9841 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     9901 ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc
     9961 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
    10021 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
    10081 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
    10141 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
    10201 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
    10261 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
    10321 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
    10381 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
    10441 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
    10501 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
    10561 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
    10621 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
    10681 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
    10741 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
    10801 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
    10861 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
    10921 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
    10981 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
    11041 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
    11101 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
    11161 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
    11221 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
    11281 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
    11341 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
    11401 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
    11461 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
    11521 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
    11581 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
    11641 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
    11701 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
    11761 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
    11821 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
    11881 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
    11941 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
    12001 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
    12061 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
    12121 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
    12181 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
    12241 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
    12301 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
    12361 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
    12421 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
    12481 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
    12541 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
    12601 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
    12661 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
    12721 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
    12781 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
    12841 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
    12901 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
    12961 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
    13021 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
    13081 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
    13141 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
    13201 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
    13261 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
    13321 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
    13381 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
    13441 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
    13501 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
    13561 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
    13621 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
    13681 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
    13741 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
    13801 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
    13861 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
    13921 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
    13981 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
    14041 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
    14101 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
    14161 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
    14221 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
    14281 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
    14341 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
    14401 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
    14461 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
    14521 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
    14581 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
    14641 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
    14701 gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
    14761 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
    14821 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
    14881 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
    14941 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
    15001 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
    15061 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
    15121 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
    15181 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg
    15241 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
    15301 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
    15361 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
    15421 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
    15481 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
    15541 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
    15601 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
    15661 catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt
    15721 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
    15781 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
    15841 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
    15901 ctttatgact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
    15961 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
    16021 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
    16081 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
    16141 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
    16201 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
    16261 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
    16321 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
    16381 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
    16441 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
    16501 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    16561 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
    16621 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
    16681 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
    16741 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
    16801 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
    16861 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
    16921 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
    16981 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
    17041 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
    17101 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
    17161 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
    17221 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
    17281 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
    17341 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
    17401 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
    17461 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
    17521 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
    17581 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
    17641 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
    17701 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
    17761 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
    17821 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
    17881 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
    17941 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
    18001 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    18061 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    18121 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    18181 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    18241 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    18301 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    18361 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    18421 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    18481 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    18541 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    18601 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    18661 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    18721 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    18781 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    18841 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    18901 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    18961 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    19021 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    19081 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    19141 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    19201 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    19261 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    19321 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    19381 tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    19441 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    19501 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    19561 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    19621 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    19681 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    19741 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    19801 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    19861 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    19921 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    19981 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    20041 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    20101 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    20161 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    20221 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
    20281 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
    20341 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
    20401 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
    20461 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
    20521 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
    20581 ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
    20641 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
    20701 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
    20761 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
    20821 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
    20881 tctgaaggaa ttactataaa aaa
//
LOCUS       DBNIBO010000031        20631 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.033, whole genome shotgun sequence.
ACCESSION   DBNIBO010000031 DBNIBO010000000
VERSION     DBNIBO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.033"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(13..996)
                     /locus_tag="AC1Y77_001024"
     CDS             complement(13..996)
                     /locus_tag="AC1Y77_001024"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIQ1574617.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="AC1Y77_001025"
     CDS             complement(1001..1648)
                     /locus_tag="AC1Y77_001025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HIQ1574618.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="AC1Y77_001026"
     CDS             complement(1733..2365)
                     /locus_tag="AC1Y77_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1574619.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="AC1Y77_001027"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="AC1Y77_001027"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HIQ1574620.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="AC1Y77_001028"
     CDS             complement(2929..3666)
                     /locus_tag="AC1Y77_001028"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HIQ1574621.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="AC1Y77_001029"
     CDS             complement(3663..4652)
                     /locus_tag="AC1Y77_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIQ1574622.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="AC1Y77_001030"
     CDS             4910..5452
                     /locus_tag="AC1Y77_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574623.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="AC1Y77_001031"
     CDS             5469..6554
                     /locus_tag="AC1Y77_001031"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574624.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="AC1Y77_001032"
     CDS             6566..7372
                     /locus_tag="AC1Y77_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574625.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="AC1Y77_001033"
     CDS             7373..8200
                     /locus_tag="AC1Y77_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574626.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="AC1Y77_001034"
     CDS             8197..9270
                     /locus_tag="AC1Y77_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1574627.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="AC1Y77_001035"
     CDS             9698..12394
                     /locus_tag="AC1Y77_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1574628.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12459..12959
                     /locus_tag="AC1Y77_001036"
     CDS             12459..12959
                     /locus_tag="AC1Y77_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1574629.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            12971..13465
                     /locus_tag="AC1Y77_001037"
     CDS             12971..13465
                     /locus_tag="AC1Y77_001037"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1574630.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="AC1Y77_001038"
     CDS             13486..14295
                     /locus_tag="AC1Y77_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1574631.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="AC1Y77_001039"
     CDS             14292..15113
                     /locus_tag="AC1Y77_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1574632.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="AC1Y77_001040"
     CDS             15164..17263
                     /locus_tag="AC1Y77_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HIQ1574633.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="AC1Y77_001041"
     CDS             17260..18378
                     /locus_tag="AC1Y77_001041"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HIQ1574634.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="AC1Y77_001042"
     CDS             18417..19295
                     /locus_tag="AC1Y77_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIQ1574635.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19441..19638
                     /locus_tag="AC1Y77_001043"
     CDS             19441..19638
                     /locus_tag="AC1Y77_001043"
                     /inference="COORDINATES: protein motif:HMM:NF016526.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HIQ1574636.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            19622..19852
                     /locus_tag="AC1Y77_001044"
     CDS             19622..19852
                     /locus_tag="AC1Y77_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574637.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            20097..20288
                     /locus_tag="AC1Y77_001045"
     CDS             20097..20288
                     /locus_tag="AC1Y77_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574638.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         6318 a         3557 c         4397 g         6359 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgtgtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccatttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    19861 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaataaatt ttttatttta
    19921 gtgaaaaata agaaactatt tctttgaagg cgataagtta gaaaaatatt attgtagtct
    19981 tttctttatt aggaatcatc actgttgtat cttattacct tttctgtttt tcttattaga
    20041 gaaaaataat ttgagaggta gatagttttt attggaggaa gtaattatgt atatttttga
    20101 aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg
    20161 gacaaaaatg gaaatttccg ccaaaagtaa ctgctcctca actgaaatca attaaagaac
    20221 tagaatttga gaatgcactg gtaatttgta tgacatgtac tgatgaagta tttcgaaaac
    20281 atttttaaag gttgagatgt atatagattt aaccaatcat ttatctctct cggaaaagga
    20341 atattaacta accagcgggt tatcaaaaat aggtaggaaa actgtactta atggcttatt
    20401 tcgcgctgat tatgagaata tcagtctgtt tgattaattt ttagggttca aaatacagag
    20461 acaaactcca ggtcgggttt cgccacaagc gaattacccg atctggagtt ttgtctattg
    20521 caggaaggcg tagccggact gcaggaataa agcccccggt tctaacaggg ggctaacaaa
    20581 gaacaggaaa ctgtgacaac tacccattgg ggagtgtcct agcagtattt t
//
LOCUS       DBNIBO010000032        20278 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.034, whole genome shotgun sequence.
ACCESSION   DBNIBO010000032 DBNIBO010000000
VERSION     DBNIBO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.034"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(36..311)
                     /locus_tag="AC1Y77_001046"
     CDS             complement(36..311)
                     /locus_tag="AC1Y77_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIQ1574639.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(554..1864)
                     /gene="der"
                     /locus_tag="AC1Y77_001047"
     CDS             complement(554..1864)
                     /gene="der"
                     /locus_tag="AC1Y77_001047"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIQ1574640.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2046..3275)
                     /gene="rpsA"
                     /locus_tag="AC1Y77_001048"
     CDS             complement(2046..3275)
                     /gene="rpsA"
                     /locus_tag="AC1Y77_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIQ1574641.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3410..4090)
                     /gene="cmk"
                     /locus_tag="AC1Y77_001049"
     CDS             complement(3410..4090)
                     /gene="cmk"
                     /locus_tag="AC1Y77_001049"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIQ1574642.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4139..4774)
                     /locus_tag="AC1Y77_001050"
     CDS             complement(4139..4774)
                     /locus_tag="AC1Y77_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HIQ1574643.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4840..6243)
                     /locus_tag="AC1Y77_001051"
     CDS             complement(4840..6243)
                     /locus_tag="AC1Y77_001051"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIQ1574644.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6230..7261)
                     /locus_tag="AC1Y77_001052"
     CDS             complement(6230..7261)
                     /locus_tag="AC1Y77_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574645.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7292..7513
                     /locus_tag="AC1Y77_001053"
     CDS             7292..7513
                     /locus_tag="AC1Y77_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIQ1574646.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7678..8415)
                     /locus_tag="AC1Y77_001054"
     CDS             complement(7678..8415)
                     /locus_tag="AC1Y77_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1574647.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8569..9909)
                     /locus_tag="AC1Y77_001055"
     CDS             complement(8569..9909)
                     /locus_tag="AC1Y77_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIQ1574648.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10146..11669)
                     /locus_tag="AC1Y77_001056"
     CDS             complement(10146..11669)
                     /locus_tag="AC1Y77_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIQ1574649.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12047..12658)
                     /locus_tag="AC1Y77_001057"
     CDS             complement(12047..12658)
                     /locus_tag="AC1Y77_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1574650.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12781..12905)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12987..13703)
                     /locus_tag="AC1Y77_001058"
     CDS             complement(12987..13703)
                     /locus_tag="AC1Y77_001058"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIQ1574651.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13709..14278)
                     /gene="scpB"
                     /locus_tag="AC1Y77_001059"
     CDS             complement(13709..14278)
                     /gene="scpB"
                     /locus_tag="AC1Y77_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIQ1574652.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            complement(14262..15032)
                     /locus_tag="AC1Y77_001060"
     CDS             complement(14262..15032)
                     /locus_tag="AC1Y77_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HIQ1574653.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15029..15400)
                     /locus_tag="AC1Y77_001061"
     CDS             complement(15029..15400)
                     /locus_tag="AC1Y77_001061"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1574654.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15419..16306)
                     /gene="xerD"
                     /locus_tag="AC1Y77_001062"
     CDS             complement(15419..16306)
                     /gene="xerD"
                     /locus_tag="AC1Y77_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIQ1574655.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(16402..16875)
                     /locus_tag="AC1Y77_001063"
     CDS             complement(16402..16875)
                     /locus_tag="AC1Y77_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIQ1574656.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(16980..17843)
                     /locus_tag="AC1Y77_001064"
     CDS             complement(16980..17843)
                     /locus_tag="AC1Y77_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HIQ1574657.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(18039..20180)
                     /locus_tag="AC1Y77_001065"
     CDS             complement(18039..20180)
                     /locus_tag="AC1Y77_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HIQ1574658.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
BASE COUNT         5497 a         4349 c         3435 g         6997 t
ORIGIN      
        1 gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc ttttaatgct
       61 ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc accagtttgt
      121 gggttgcgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa accgattaat
      181 tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc atctactgct
      241 gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat taattctgct
      301 ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat atcatttagt
      361 taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt caatattctg
      421 ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt cacatgcttt
      481 ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa aaaacgtcga
      541 tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc ccttcaaaag
      601 taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc atcaattcct
      661 cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta gcataaaaaa
      721 tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca tccataatga
      781 tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact tgttcgatca
      841 tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg attggagcgt
      901 aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt gtattggttt
      961 ctttctttac aagatcccat ttattcacga cgataataat tccacgacca gcttcatggg
     1021 cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc agtaccatca
     1081 aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat ttttcagtat
     1141 tttcgtatac ttttccgcgt ttacgcatac cagctgtatc gatcattaag aatttctgac
     1201 cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca atatccgaaa
     1261 caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca acatttgggc
     1321 gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct tcagagaaat
     1381 acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct gaaacaggat
     1441 aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt tctggattat
     1501 ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt ttcgcaacca
     1561 tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt acatctgctt
     1621 cttcaatcgc gatttctgct tgatgcttga tctgatccat aaagggttca tcgccaagat
     1681 caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct tttgcataaa
     1741 tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca ccagcgatgc
     1801 ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt gttggatttg
     1861 ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat gcattgccaa
     1921 ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga aatactaatc
     1981 gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca cagatacaaa
     2041 atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt caacgcatca
     2101 cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata agactcaaca
     2161 ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact caatgcaata
     2221 cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc ttcatgaagc
     2281 acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac tagcccttct
     2341 actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac tgttccgtct
     2401 aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg ctgtgtgtct
     2461 ttgatggaca aggaaacacg ttcttgttca ggattgatag acaacacttt gacatttaca
     2521 tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga aatctctgag
     2581 acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc agtaagacgt
     2641 gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc ttttttcttt
     2701 tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt ttctgatggt
     2761 tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa gtctgcaaca
     2821 aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc aacgactaaa
     2881 ccgcctttga caacatttgt aacaggtgct tcgatcacct tgccctcttg gaaatctctt
     2941 tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag gtagctgccg
     3001 ttttctttat ctttgccgat cgtactgata acgactaagt ctaatacatc accgactttt
     3061 actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg aacgactcct
     3121 tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac taaaacttcg
     3181 cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc ttccattgac
     3241 tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct aaccaacatt
     3301 ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa taaagcgatt
     3361 tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt taaaaaagaa
     3421 gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga atcgatttgc
     3481 cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg tcccttgttg
     3541 aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca gtcggaattc
     3601 ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt gctactaaaa
     3661 agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg tccatgacaa
     3721 cgccaccgcg ttcccctatt tttcgttgga tagcaactaa ttcttctctg acttttccat
     3781 gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct aaagtcacat
     3841 cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag ataggatact
     3901 ttttgatcag cgaaactagc ccttcttcat ctgaaaagga tacattgttc gtaatagcta
     3961 aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc aattcttttg
     4021 ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt gcaatactga
     4081 tttgtgtcat gatataactc ctttttctca aaaaaaaagc cgcaaagcgc ggctattatt
     4141 atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct aagccattca
     4201 attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt ggaccttcgc
     4261 ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt gtttctgctg
     4321 cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc ggtgttgtct
     4381 ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta gattcttccg
     4441 cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt gtacttgtag
     4501 aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca ttgttgcttg
     4561 atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag accagtaaaa
     4621 tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga gaacttcttg
     4681 atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt gtttcgttat
     4741 tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa ctctatcgtg
     4801 tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca tgtttttttt
     4861 aaataaattt agtaaaatcg tttcccaatg aagttgttca gtcttagaaa aaagcttcct
     4921 ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg gcttttcgga
     4981 gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt cattaatata
     5041 gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgatgtttga gccgttccac
     5101 ccaattttct ggtttttcag actgctggat cagctccatc catttttttt gcagctcatc
     5161 aaagggagct tgctctgtgg catattccaa ataaagctca aaactttgtc gctgttctct
     5221 agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa aaagaattgc
     5281 tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt tttctagact
     5341 gtctggcaaa tcataatgga tcacataacg gatatcagat ttatcgatcc ccataccaaa
     5401 tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt gaagttgtct
     5461 tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt ccctgaataa
     5521 atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg ctccttgtgc
     5581 tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa caaaaagcga
     5641 gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa atagtacatg
     5701 acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg tgaccgggtt
     5761 tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat caactcccca
     5821 ttgagacacg caatgagcct catctactac atataacgca atattttgtt ttttcagatg
     5881 ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt tatattgcga
     5941 tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga aagcagctac
     6001 tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag aaagcagcgg
     6061 tgacacgatg atgaccagac cttccattaa aaaccccgtc aattgatagc aaaggctttt
     6121 tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg cctgaatgat
     6181 ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt catttattaa
     6241 cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat tctccataat
     6301 ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa tccttatttg
     6361 gttccaaacg ttcgattgtt tctgcagaga gaatccgctc gtatggaaaa tcatcaaaaa
     6421 acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga cggattgcca
     6481 taatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc atgctttgat
     6541 tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct ggccgttttt
     6601 cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc gctgactcat
     6661 cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca gataattggt
     6721 tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc aaactctcat
     6781 acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat ttttttagtt
     6841 ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc gttgccagat
     6901 tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct gttctgccat
     6961 agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata tcagcccctg
     7021 caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc gcaatttttt
     7081 gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg ataaacaaaa
     7141 gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca aggagttggt
     7201 aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata aaaacaatca
     7261 tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac ttgtacctga
     7321 acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg attatgatga
     7381 agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg tgatgccgga
     7441 agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga ttttattaga
     7501 aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc taaacttgaa
     7561 aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg aaagattcat
     7621 ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc atttatatta
     7681 tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct ggtctgcgtt
     7741 agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac ctccatgatt
     7801 tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag cttcttgata
     7861 agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag gaacagctga
     7921 ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc cttttaaatg
     7981 tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta atgaatggcc
     8041 accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt tgtttccgcc
     8101 catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt tttttgctaa
     8161 atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat aaaaaataag
     8221 taatggagaa tttccatgat tctttttctc tccttcaaaa aataagtaat cttctttgtt
     8281 ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat tttgctgtat
     8341 atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa ttagtattcg
     8401 tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt tcttaatcaa
     8461 gtatgattat accttaaaaa aaataagaga gaaatgcaaa ctttccagca tcaaataaaa
     8521 aaagacggaa tatccatttg aatatccgcc ctttttgtat gtttattttt atttagcaca
     8581 taaacgatac acagcttctg cataaatttc cattgcttta tacatactac ttaattccca
     8641 tcgttcattc gcctggtgca taaagtcagg agtatcaggg aacatcgcac cgaatgccac
     8701 acactggttc atcgtacgag caaaagtagc gccgcctgat acaaacggtt cagtcatgtc
     8761 tccagtaata tcgcgatatg tccccaatag atttttcact aactcactgt ctagtggcac
     8821 gtataatgaa tcaaggaagt caaattcttc ataagtcaac ccatattttt ctgctgtttt
     8881 agttaattta gctactaaat catctttctt gaatgtaaca gggattctca tatctacacc
     8941 gatttttgtt tgttctggtg tgatttccaa acttgcaaag ttcatcgtta gctcgccgga
     9001 ttgttcatct tctgttttgc ccacgacagc ttcaccagtt gcattttctt gaactaattt
     9061 tcctaagaaa ttgattgggc caaaatcgaa tacttctgaa agagcaattg ccagacgaga
     9121 aatagcattc actccttgtg ctgcatcttt cgcgtggata cttttaccaa gaacgacgat
     9181 gccttcacct tgttcttcga aatcgaattc gtgttttttc aacgcttctt ttacttcaga
     9241 aagcttttct ccagagtaag gagctgcatc aggaacaaca ttcaacgctc ctccagcttt
     9301 tacagaaaac tcgttcgttc ctggtcctgt caagtaagct tgcaataagc ctttttctgc
     9361 ataaattaaa ggaaattcag catctggcgc aaatccttgc gtaattcctt cttctttttc
     9421 gttatatttt tctaaacaac gccataaatt ttcttcatct gtaccaaaga taaaacgaat
     9481 acgtgtcttg aattccacgc cagcatcaat caaagctttg acggcataca ttgccgcaat
     9541 agaaggacct ttgtcatctt gggatccgcg accaactaac cagccgtctt ttatagttgg
     9601 atcgaatggt tttgtttccc aattattttc gtcccctgct ggaacaacgt ccatatgaca
     9661 caagataccg aataattcat ctccagagcc aatctctgaa taaccgtaat agccttctgg
     9721 atctttaaat gttctaaatc ctagcttttc actgatttcc aatactttat ccaaagcttc
     9781 aatcactttt ttaccgaagg gatgtccttg tcctgaatct gcttcatcta gtacggaagg
     9841 aattcggatc aattcgctca atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa
     9901 ttttttcatg atattatcct cctaatatgt taaaaagggt catgtcaaat tcctccgaca
     9961 caacctcttc ttctcaaata atgacgtttt aagcctgctc catcagcaag cagaatgttc
    10021 ttaacaaatg atgaaagaaa aaacactcac tgaatggagc tattgacttg tccacgtgct
    10081 tatcgatagc cgtttatcgg tttcttcatt cttatgatag accttgtacc tgtatttgtc
    10141 taggtttaga acattgctgc tacacctagg atcacacaga tggctacaaa taatatcgca
    10201 attaatttac cggtaaattt ccaccacact gaaacatcga aacgagcaat tgctacagca
    10261 cccattacga tagcagatgt tggtgtaatt aagtttacta aaccagatgc agattgataa
    10321 gcagtgatta ctaaatcctt accgacgccg gcaaattcac ccataggtcc catgattccc
    10381 attgttgcag cagctaagcc ggaagttgaa ggaatcaaga atgacatagg gatatagaaa
    10441 ataaatgtaa taatgatgaa tacacttgaa gataatgaac ttaatccttg ttcgccccaa
    10501 tgtaagatgg tgtctgtgat taatccattg ttcataacca cttggatacc acgagcaaca
    10561 gctactacga ttgctacacc gatcaaatca gaagcaccag ctaaaaattc agagacaaat
    10621 tcagactctt tcatgccata agtgattgct actacgatgg acattactaa gaataacata
    10681 gtaatttctg ggaagtacca gtcacctaat ggaagcatgc ttttacctag aacagtacct
    10741 agtaccggaa ggtttgttaa ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc
    10801 aaggttgtcc aaggaatcaa actgatgatc atgataccaa aagtaataaa gaacaataca
    10861 ttaacatgtt tttgtttttt cgtgattact tcttgacgac cgattgcttc aatatcgaaa
    10921 tgcttcatat cttctttacg ttggttgtat actaatgatt ttgttggatc tttttcgatc
    10981 tttgatgcat aacgatacac atatacaata gaaatagcta acaaaataac gaacatcaag
    11041 aatcgaagac caataccttc acccatcgaa atcccaacag cttgtgaagc tacgcctgtt
    11101 gcaaaaggat ttaccgttga agctagacag cctacttgag accctactaa tacgattgct
    11161 actgctacta ctgtatcaaa gcctacagcc atcataactg ggatcagtaa agcatagaat
    11221 ggaatcgttt cttctgccat tccgtaagta gagccgccta atgcaaataa cagcatcaat
    11281 actggaataa gcattttttc ttttcctttg aatttcttca caactgaagc aatcccggca
    11341 tctagtgccc ctgttttgtt gataatacct aaaaatccac caacaaccaa aatgaagaaa
    11401 gagattggga ttgcagcagg tgtttcttta gttagctccc ccgctggtac acctaacatc
    11461 ccattgattg gcgccatgaa aatatcccac agcccttgag gattttgttg tactcgttca
    11521 tatgatccag cgacgatgtt gcctgcatcg tctacactat agtgcccagc tggtataaac
    11581 caagtaagta cagcgattac tgcaataata ataaataaga ccgtatacgc tgaaggcatt
    11641 tgaaattttt tcttttttgt ttgttccatc ttcttcttcc tttcttttcc gttagagaca
    11701 acaattttgg ctctaatgtt attttatcaa aaagagtatc agaacaaaaa taataacacg
    11761 cattttgtaa aacttttgtt caaaaagttc gtttttccct attccctcct cttttttcag
    11821 gtttttaaag ctttcttctt tttcattgta gcgtttcctt ttttttataa acaaacattt
    11881 tcgtctttta aaaggagaag aatttcacat ttgcaaagaa ttgattgatt tttatgcata
    11941 aatcaaaaaa atactcaaaa caaacaaata aaacgtttac attattgcat gtcttgtatt
    12001 tttttccaaa aaaaaagacc aagaaatttc ttgatctttt tgataattat ttgactgctg
    12061 tatgacgttg ttcgaactga tgttgttttc gacttaacca tggaagaagt tgcgcatgca
    12121 gcactaaaaa gacagcgctc acgatgaatc ctttgatcaa gttgaaagga acgatcccaa
    12181 tcagtacata gtttgctaaa gacatgccta gcatctgtgt tgcgttcagt cctaaaaaca
    12241 ttaagtaaag aggcgtaata acgaaataat ttgcgatact cataaaaata gttagagcga
    12301 tggttcctgt aatgacacct aaaaatttgt ttcttccttt atgctgtttc tttacactaa
    12361 agaaataata aatcgggaat gtaaagatcg ttgttgataa gaaactagca gtgtctccta
    12421 ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc aagaacgctg
    12481 ttgcaactcc ggccgctgga ccgaataaga acatgctgat caataccggg atatcactaa
    12541 aatcgatctt caaaaaacta aaagccggca tgatcggaaa agcaaaatac tgtaaaacta
    12601 acgcgatcgc agcaaacatc gctactgcaa ccattttctg tacacgggtg ttcttcattt
    12661 gaaatcctcc tgttaggact cctcttcagc gaactttttg acgtttctct ccaaatgaaa
    12721 aaactctatt ttccagggaa aatagagtta gagttatttg atcaagcgtc gtcaaataag
    12781 ctctatcttc tttatccaga ctatactgtc ggtatcggaa tttcaccgat tcagctattt
    12841 tttgcaaaat aggtcgtgga ctataaccac cggtcgggaa tttcaccctg cccctgaaga
    12901 cgaaccttat ataatttttg tttacatatt cattatacat atctattcaa aaaacgcaac
    12961 atctaacact tataaaaagt aagtgttcat tttgagtggt tcagcaaact gctgacttct
    13021 tttttgttca gttcacggta atctccagga cgtaggcctt gtaaagtcag atctccgtat
    13081 ttttcacgtt ttagtttttg cacaggaaga ccaactgctt gaagcatatt tttcacttga
    13141 tgattacgtc cttcgtggat cgtcaattcc acgatactgt ggtttgtctt aggatccact
    13201 gataaaattt ggaatttagc tggagatgtt tttcgtcctt cgatctttat tccttttgtc
    13261 aaaggtgcaa gcatcgattt tgtagcgatc ccttttacct ttgcaacgta aactttatcc
    13321 acttcatgtt ttggatgtgt caggcgctga gcaaaatctc catcatttgt cagtagcaag
    13381 atccccgaag tatcgtaatc cagtcgacct acaggataga tacgttcttt taccatcggc
    13441 aaaagatccg taacaacttt tcttcctttg tcatctgcaa cagcagaaat aactccttta
    13501 ggtttgtaca ataaataata accgtattct tcttgataaa ttgggacttc atccacttcc
    13561 acagtgtcat gtttcccaac ttttatccct aattccctta caacttgtcc gtttactttt
    13621 acacgtcctg caaggatgaa ttcttctgct tttcgacgag aagtgatgcc agcgtgggca
    13681 attactttct gcaatctttc cattacaatt attctccttt attcattcca tcaaagaata
    13741 gatctggtga cacgtcttct tcaaatgaaa tttccatttc ttgtgtatca ggcagttctt
    13801 ctaatgattt caaaccaaaa taatctaaga agtagccagt tgttccatat aatatcggcc
    13861 gacctggccc ttctactcta cctttttctt caatcagttt atgggcagct agacgctgta
    13921 ccgctcctga aacctgaact cctcgaatct gctcgatttc agctcttgtg attggctgtt
    13981 tataagcgat gattgctaag gtttcgacag cggcttgaga taatgtgttt gatgtccctt
    14041 gcgcatatcg cttaagtaag ggagccagct cttttttagt cgttaataca taatggcttt
    14101 ccgtttcaaa aatatgcaaa gctgagtgcg agttttgagt gtaagattct tttaattctt
    14161 tcactaaact tgtgacttct gcttcgctga gatttaacag tccgcttaat tcttcattac
    14221 tgatcccttc ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat ttcgtcatgt
    14281 tcggtatctc ctactgctgg ataaagcata attgattcat agttttcttt ttgttgcgca
    14341 atgatctgcc cttttttcat caattctagc aaagccataa aagtagtgac aacttcactt
    14401 ttagaaggtg actcaaaaaa agaatcgaaa gtacagccat gcgcatttct ttttatacgc
    14461 tcttcgataa ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat cgttttttcc
    14521 actttttgag aaaatttctt acgttctaag attttctgca tggcacggat caaatcgatt
    14581 tttttgactt gtcctcgctt caacggcttt tcagcatccg ctaacccatc gatgttcata
    14641 ggttcttttg tgtaataaag acttcgttcc tctgcttttt ctgtcagtct ttcagctgca
    14701 tatttatatt tacggtattc taaaagctga gtgaccaatt cttctcgagg atcttcttcc
    14761 cataattctt catcatctgt gacgaattct tggacaggaa gcaatgtctt gcttttgatt
    14821 gccatcaatg tagcagccat cactaagtat tctcctgcaa cagctagctc tagtgttttc
    14881 attgtatgga tataattcat atattgctcc gtgatcgtag cgatcggtat atcatatata
    14941 tcgatttcca tttgctgaat cagatgaagc agcagatcga gaggaccttc gaatacatct
    15001 aatttaatat tgatctccgt caactcattc actctttctt ctttttcttt gctagccatt
    15061 ttccaatgta aggctctgtg atttcagttg cacggatgga gacatccggg tacttttcct
    15121 gtaattcatc taacatttga aagcccatat tctcaccgcg ataagacgga ttcaaagaaa
    15181 tgtcatggat gatcagctga tcgttcgttg cccagtaaac accaataatc ccttgaatgt
    15241 tggttgaacc ttctggcgta aagaaaaaaa gttcataatc gtcttcagat tcgtacgtat
    15301 caatctcctt caacaaagaa gttttacctg tcagtttctt atggaaactc aatagtccca
    15361 tagcaatttt acggttttct ttccggtaat gtgttaacat cttctcacct tcctatgact
    15421 aagcccttgg aaaatgctgc ttataaacat ccgtcattct tttcttcgtg atatgtgtat
    15481 aaatctgagt cgtagagata tccgcgtgac ctaacaattc ctgtactgtc cgcaaatcag
    15541 ctccattttc tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt gttacttctt
    15601 tgtaaatccc agcctctcgt actagctgct tcagattttt ccagattcct tgccgagaaa
    15661 gacctttccc atgattattg acaaatacat gcatttcaga tgcgtccttt acaagtaatg
    15721 gacgtgcttc gtctaagtag cgttccagcc attggatcgc ataatcacct aaaggaatga
    15781 tccgctcttt atctcctttt ccaactgtct gtaccagtcc aagagaaaga tgaagatctc
    15841 ccaatttcaa tccaatcaat tcactgacgc gcatccctgt agcatacatg acttccaaga
    15901 ttgcacgatc tcttatccct aacgtttttg tcgtatcagg tgcttcaatc agcttttcta
    15961 cttctgtcag agtcaatgta cttggtaatt tctgtgcttt cttcggcgta tcgatatgtt
    16021 gcatcggatc atgatcagtg atacgctctt gccgtaaaaa ctgatggaat cttctgagac
    16081 ttgagatcat ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac ttctccagaa
    16141 aacggatgac taaaaagcga tcgacatcct gccatgaagc aattttctgc tcctgtagaa
    16201 aatgcaagta ctgttccaaa tcacgttcat agctttgtcg tgtattggcg gacaatcccc
    16261 gttcaatatt caagtagtgg agataatcgt tgatttgctc tttcataata atccccctca
    16321 tgcctctcta tcatatcaga taccgcttca ttctgatagt ctttttccat aatctgagca
    16381 aagaaaaagc cgtctagaca ctcagacgga ctggtttctt ttttcttttt catagcattc
    16441 ctgacagatt ccatggaacg ttagccgatg atcttttact aaaaaatgat aacgttcttc
    16501 tactacttgc tccacttcaa gtaaaagatc ctcttctact tcctcgatcg tgccgcagtt
    16561 caagcatagt aaatgatgat ggaaatgttt ggcgccttct tttctcaaat cataacgggc
    16621 taatccatca ttaaaactta ctttatcgac aattttcaat tctgtcaaga tttcaagggt
    16681 tcgataaaca gtagcaagtc caatttccgg actctttttt ttcactaaaa agaagatttc
    16741 ttctgcagat aaatgctctt tctcattttc caaaagcaca agcagagtag cttctctttg
    16801 cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt gtttttttca tcgccgaaat
    16861 agcattcatg accatttact caaccccaac aatttataat aattacaaat aaaaaaagat
    16921 ataacactat gttaattaaa attattataa gctagttaag aaaaacatgc aagggattct
    16981 caattagatt ttttgactag ttttgtcaca ccgttttctt gtgtaatcaa tccttgtttc
    17041 aaaagattac ctaatgcgcg cttaaatgct cctttgctga taccaaaagc tttcatgatt
    17101 tcttctggat ccgatttatc tgtaaagttg atttgctgat ctgcagaacg ttctaaaatg
    17161 gttaaaatca tttgtgcatc atctgatata gcttcatatg ctcttggctt caatgaaaga
    17221 ttcagtacac cgtctggtcg gacaccaatc actcgacctt ttatttgctc gcctagacga
    17281 ggttcattat atcgttcaga aggatggata aacccaagat agaaatcttc cgttaaaacg
    17341 tacgtacctg ttaatttcaa gcggtatgct gttcctatca catctttgtt tttcatttct
    17401 tctgttccat atttgcttaa agatttgaat attttttcat ctgctaaact gccccaaata
    17461 cggtcttttg catcaactct aagggaaatc atcaagcgat cccctttttt aggccatagc
    17521 tctgtcattg tcggcaattc atcaagagat acgacaaagt ctttatctgg aagaccgata
    17581 tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga ttccaaaagc gtaatgaccg
    17641 atacggcttt taggaatttg tgtagtgatc cgattttcat gtttttgatt ttggtagccg
    17701 aatccttcca ctgcttcacc gattttgtgt tcaccttctg atttgcttaa gccaaaggtt
    17761 tgtccgtttt tttggacaaa gtagtttttt tcattttcat caataataat acctgtaaat
    17821 atagttgcta ataaatcgtt catttttttc tccctctata gtgaaaggtt ttcttaatac
    17881 atttctttgc tatcataaca taatgttatc tacttgaata gctgagcctt ggtttggaaa
    17941 ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc ctttttcgaa taaacggtgt
    18001 taaacggcag gctcacagcc tctttcattt gcctaaaatt aattgctccc atttcctccg
    18061 aaccatccag ttttacagtt gatggttacg atgtaaaatg gcaaagcact ttctccaatg
    18121 cctttcatca atgcagattt tgttgaagtg gcatatagat tgtaatagcc ttccccattt
    18181 tcgattctgg ctttttctaa tctgtatcct ccttcaacga tatatccacg ttggatacat
    18241 gtagcaatca ctttgctttg gacacctggt gcccacgtcc atgctggatt agatgcatcg
    18301 gcaatatcac ttgctctggc agatttgatt ggctgattcg ctgagtttga agaggaaggg
    18361 gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg cagcttgttg agcggcttga
    18421 gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt cagctgcttt tttcgctttt
    18481 tctgcttttt tcgcttcttc tgcttttttc ttttcctctt cttctttttt ctctttatca
    18541 gtgagcatct tatctacttg ttgcaattgt tcattcaact gagcagatag atctgtattt
    18601 ttcacttttt ctactgcttc ttttgccgtc ttgtattggt cgcgtgttgc tgaatccact
    18661 acttttccgt ctttaaagac tatagctgtt ttttctttag ctgtttgcaa ttcagcatat
    18721 tgatttttag cttctgtttg tgcttgttga atcaattttc caaaatctgt gttatccgtt
    18781 tcgattgctt ggatctcttt atctgctttc aacttgatgt catctgctag atgatcatcg
    18841 acgatggcag gtgctatgaa ccactcattc actttttgga tatctcggat cttagattgg
    18901 atatcttggt aagttgtttc taactctgaa tatccttttt cattcttaag actggataaa
    18961 tcatttttta gttttgataa atcttgatga atcatatctg cacgaataaa ttgatggtga
    19021 tcatctgtat aaaaggcagc aagtttctca tgaagttctt ccatttgttg ctgtcttttt
    19081 gcttctgctg ctacttcggc ttctactttt tgatgatggt cataataagc ccagcctccc
    19141 ccacctagta gaagaactgc tgctaaagcg agatacggca ctttttttga ttttttcttt
    19201 tgtgtcttat ctaaatcttt tttttctggg tttgattctt gaggcgtttt ttcagtattt
    19261 ggcacgattg ttattggttc tgtttctgct tcaatcagct caggctggtt ctcttctttt
    19321 tctattttag cttcaagagc agtgctatct tcacttgcag attgagtttc agcatcggct
    19381 tcattattta ttccatccga ctctgttgcc ttcttgtctg ctttttcgag catttctttt
    19441 ttatctgctt ccggcaaccc attcgatttc tcgtcttctg attgtttttc agacaatgtt
    19501 tcagtttttt ccgggatatc cgtttttaga tcattcgttt ttagatcatt cgtttttaat
    19561 gattctttac ctgcagcgtt tactccacta atgtcttttt cagtgtcttt ttcagcttca
    19621 tgttctggct ctactttgtt ttcctgtgca attttttcac tgatcatttt gagcagctct
    19681 ttcgcgtgct cttcttttgt atcttcacga tgtgctttga tataagcgga tagtatcgaa
    19741 ttttcttcct cattatcgac gatttctttc agttcagcta ctcttctttt ttgttttgat
    19801 tcaagcgttt cttcttgatt ctttcctgat tcttccgaag tctcatgaac agttgctttc
    19861 ttctgttcat ttttctctat attagttact tgatcgtgag caggtggttc ttcgtcttct
    19921 ggttctgctt tttcgaataa ttccattaca gattctagag gtaaatcttt caattccgac
    19981 cattcgattg gatcgtttaa ttctgtatca ggaaagactg catcttcagt tgatttatcc
    20041 tgcatagctg catctgtatt tgtttcttca attgattctt ttatttgctg atcttctgca
    20101 gttatttgat gaccgcagtc tggacagata gtattctgct ccattttttt cgatccgcaa
    20161 tttggacatt ttttcatcac ttagcgatcg gctcctttac taggtttacg ctaccattta
    20221 atctatcata ttctatacgt cacgccaata gcttattttt aggatttact taaaaata
//
LOCUS       DBNIBO010000033        20220 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.036, whole genome shotgun sequence.
ACCESSION   DBNIBO010000033 DBNIBO010000000
VERSION     DBNIBO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.036"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            66..1100
                     /locus_tag="AC1Y77_001066"
     CDS             66..1100
                     /locus_tag="AC1Y77_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIQ1574659.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            1097..1864
                     /locus_tag="AC1Y77_001067"
     CDS             1097..1864
                     /locus_tag="AC1Y77_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIQ1574660.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            1964..3532
                     /gene="liaX"
                     /locus_tag="AC1Y77_001068"
     CDS             1964..3532
                     /gene="liaX"
                     /locus_tag="AC1Y77_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HIQ1574661.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3708..4016
                     /locus_tag="AC1Y77_001069"
     CDS             3708..4016
                     /locus_tag="AC1Y77_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIQ1574662.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            4016..4366
                     /locus_tag="AC1Y77_001070"
     CDS             4016..4366
                     /locus_tag="AC1Y77_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIQ1574663.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            4532..5470
                     /gene="hprK"
                     /locus_tag="AC1Y77_001071"
     CDS             4532..5470
                     /gene="hprK"
                     /locus_tag="AC1Y77_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIQ1574664.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5485..6315
                     /gene="lgt"
                     /locus_tag="AC1Y77_001072"
     CDS             5485..6315
                     /gene="lgt"
                     /locus_tag="AC1Y77_001072"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIQ1574665.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            6359..7384
                     /locus_tag="AC1Y77_001073"
     CDS             6359..7384
                     /locus_tag="AC1Y77_001073"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIQ1574666.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7399..8337
                     /gene="galU"
                     /locus_tag="AC1Y77_001074"
     CDS             7399..8337
                     /gene="galU"
                     /locus_tag="AC1Y77_001074"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIQ1574667.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8462..8901
                     /locus_tag="AC1Y77_001075"
                     /pseudo
     CDS             8462..8901
                     /locus_tag="AC1Y77_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            8904..9518
                     /locus_tag="AC1Y77_001076"
     CDS             8904..9518
                     /locus_tag="AC1Y77_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIQ1574668.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9581..10684)
                     /locus_tag="AC1Y77_001077"
     CDS             complement(9581..10684)
                     /locus_tag="AC1Y77_001077"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIQ1574669.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10894..11895
                     /gene="ccpA"
                     /locus_tag="AC1Y77_001078"
     CDS             10894..11895
                     /gene="ccpA"
                     /locus_tag="AC1Y77_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HIQ1574670.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12249..12644
                     /locus_tag="AC1Y77_001079"
     CDS             12249..12644
                     /locus_tag="AC1Y77_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574671.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            12641..13897
                     /locus_tag="AC1Y77_001080"
     CDS             12641..13897
                     /locus_tag="AC1Y77_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HIQ1574672.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            13909..14232
                     /locus_tag="AC1Y77_001081"
     CDS             13909..14232
                     /locus_tag="AC1Y77_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574673.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14225..14491
                     /locus_tag="AC1Y77_001082"
     CDS             14225..14491
                     /locus_tag="AC1Y77_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574674.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(14654..16999)
                     /locus_tag="AC1Y77_001083"
     CDS             complement(14654..16999)
                     /locus_tag="AC1Y77_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIQ1574675.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            17278..18180
                     /locus_tag="AC1Y77_001084"
     CDS             17278..18180
                     /locus_tag="AC1Y77_001084"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIQ1574676.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            18391..20166
                     /locus_tag="AC1Y77_001085"
     CDS             18391..20166
                     /locus_tag="AC1Y77_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HIQ1574677.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT         6647 a         3604 c         4219 g         5750 t
ORIGIN      
        1 ggaaaagggt ttgtgacaaa acatgtcaca aacccttttc attattccaa tagaaaggat
       61 aaagaatgca aaaaaagatt agcatcatca tcactctatt ttttatttgt atacttgctt
      121 catggtttat tcagcgtcaa gctgctctat atgacgtacc tgtcatcgag atcgaaagag
      181 tgagaataga agaggatcaa caattgatta caggaagatt gatgaatacg gaccaaaaag
      241 gagaagtgat ttctttaacg gctccttatc aaaaaaatca aatcgaaaca gcaaatataa
      301 caacaagaca aatctatctt gtccaaatgc atggagatga atggcaacta gtagcaaaaa
      361 aaaatgatgg ctggcttttt ttctttacaa gtgtcttcat tggcaccatg ttgcttattg
      421 gtggaaaaca gggcgtattt gctttaggaa gtctcatcat caacatcgtt ttacttttac
      481 ttttgttgct cctctttacc aaaacagaag gtatttctct cttaaatata gcaatcttat
      541 ttgtcgttat agggataatc gttagtatat tggcgttaga cggatggaat agaagcagta
      601 taatcaaaat ttcggctgca gttacaagtg tttttttagc attttttatt tgtcttttga
      661 caatgaatca ttggaaagat caaggactgc gctatgaaga actgaattat ctgacacgac
      721 cgtatcgttc tgtttttctt tccagcgtgc tgattgggac agctggaggg acactcgata
      781 ccgtgatcac agtcattgcg acattagaag aattgaccaa gcaacaaccg aaaatatcag
      841 cgaaacagtt atggctttca ggaaggcaag tgggccgaga tgtgattggt tcgatggcta
      901 atatcctttt attcgtctat atcagcggag cgattccatc gcttctgtta tatttaggaa
      961 atcaatggtc tttcaaagaa acggtagaac gacatttatc attagaactt ttacgcgtca
     1021 ttgctggcag cttaggaata gtattatcaa tccccatcgc ggtcctcttt tttctcgctg
     1081 taaggaggtt aaaaaaatga atgtacttat tatacttgtt gggatttttg caatcagtgt
     1141 gctatttgtc ggtggtacgc aaggtatgta tattctttta ggattattca ttaatttagg
     1201 aatctttttc ctattattat tcggctatca tcagaagtgg ccgatattgg tattgtcgat
     1261 tatagggttc ctattgatcg ctgtggttat cttatttttt atcaatggtt ataatctgaa
     1321 aatgcgagcc gcttttgcca gtatactgat ctttttgttt tgtttcctac tattgattcc
     1381 tatcactgat tttttagcga tccaaggctt tacttcgata gaactagaag aactatctgg
     1441 cttagataag acgttagcaa ttgattttag actacttacg aggtcgttgc ttctgatcag
     1501 tcttagcgga gctgttttag atgcgagtgt agcgatttct agtggaacat tcgaagtata
     1561 tcaggcaaat ccgcaaatgt cttttaacca attgcgccat gctagctttg ccattgctaa
     1621 aaaaatattg gcatcaacag tgatgacgct tctttttgcg tttatgggga gttctttagc
     1681 gttgattctt tggtttattg acttagacat ttcttttcag caaataatca atgaaaaatc
     1741 atttgtcttg gaatatacga tggctattct taccacactg gccgcactac ttgtcctgcc
     1801 cttaacttgc gtgactacag catatctctt tacaaaaaag aaagaacatc tcaagatgga
     1861 gtaaaagtta caacttaagt cctatgacat ttttactgaa tcagtgcata atatggatgt
     1921 aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag aaagagaacg
     1981 tattttagac ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag atttattaga
     2041 aagcatggca actgaaaaag acgaaaaaca aatcaaaaaa gaagcagaca aggtaactgc
     2101 tagtcataaa gaaaaagatc aggcaagtca attgatcgat gaacttgaaa acggtgaaga
     2161 aaaaatttct gagccagttg atccaaaaga aaaagaaaga caagatcaag aaaacttaga
     2221 aaagatctta gatgaactag caactgaagc gaataaaact tcggcgcgtc tagacgaagt
     2281 aaatgctcaa ttcgctgaca ataaggcagc acgtaatgag aaacaggaag cattgatgca
     2341 gctgaataca aaagaagaat taggcgagtt gacagaagaa gaattagcac agcgtcaaac
     2401 gttagaagca gaaatcaaag agttagaagc aaccggcgat acactattag aagaacaatt
     2461 gaagctggaa gcagaattaa aagatatccg caagaatcaa tggtctgaga aaaaagaaac
     2521 atttacggat aagtttgaat tgccagatga ttggaaagat caagcgacag atacgctgaa
     2581 tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta ggcaaattct tgaagaaaac
     2641 attccaaact gtttcagaaa cagtaaatga taacatggaa tggaaagatg tcagcttacg
     2701 cgtccctgga attgcgacaa cgaaatttga acacgaattt tattatgaag ctcctgcagc
     2761 aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt gggacagtga
     2821 tgatgtgaaa gtggaagcca agatcaaact ttacggaaaa atgggtgctg aaccatttga
     2881 agcattctct gaacggagcc agatcgaggt caatgaagat catatctcct tccaaattcc
     2941 aaataagcgt gtgcgtgcag accttgtttt ctacttgcct aaacgagtat acgaccatgc
     3001 agctatcaaa cttctaaatg gcaacatcat gattgagaca ttggaagcta aagatattta
     3061 tacaaaatct acgaatggga acatcatcgt gaaccaatta actgcaacga tgctggaagt
     3121 agaaggtgta aacggcaata tcgatattcg aaacgggaac atcttagatt ctattataga
     3181 aacagtaaac ggtacagtga catttggtgc cacaccagaa aacctttctg tgtctttagt
     3241 aaatggtgat gtacgtctta cgattaaaga agataatttg aaaaaagtag aagcaagttc
     3301 agtgaacggg aatgtcaaag ttgctttacc agatagcatc ggtttggaag gacacgctaa
     3361 aacaagtctt ggaagcatta acagccgtct gtccaattac gaagtagttc gtgagaaaaa
     3421 agaacggaca aaccaaatgt tgcaattccg tcgtgtttca gatgatgaga tcgcacaagt
     3481 gcagctttca acgacaacag gaagtatcta tttaaaagat acagataaat aaagaggttg
     3541 tgaaagagat gttcagcctt gagcaataag aagcgatttt ttaaaatagc ttcccctatt
     3601 ttaaaaaaat gtgccttatt gccgataggc tatctcttga acaccgatta agaagaaggt
     3661 tgtcacaacg attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa
     3721 agttgacaaa atccaataaa aatgttgttt tgacaggtac tttagcaggg attggggaat
     3781 atatcgggat tgatcccact gttatccgtg tgctttatgt attcttaagc ttagttgctt
     3841 ttggaagtcc aatcatttta tacattttat tagctttgat tatcccatct gcgcgttctg
     3901 cacgaacaaa ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa
     3961 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
     4021 gtactttcag cgtattttgg ttaatacgtt aacattcata tcgttggctg ttctttttcc
     4081 tgcgatggtc catgtgagca gtatttggac ggccgtcctt gccagcttcg tattatctgt
     4141 gttgaacatg tttgtcaaac cgattttgac gatcctgtcc ctgcccttta cattagtcac
     4201 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgactt ccttttttgt
     4261 aggagcagaa cattttagtt tttcttcatt tggtgcagcg ttattcgtag ctatcttgat
     4321 gtcacttgtc aatgtaattg ttagtgaaca taacagcaga aaataaaaaa agctgtgccg
     4381 agatctcggc gcagcttttt tgtttagaat aaagaatgcg ttttctatta cattggtaat
     4441 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
     4501 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
     4561 agtaaaaaaa ttatctttgg aaatcgtcac aggcgacgag aaaagtttag accgcgtcat
     4621 cgtgactggg gacatctcac gcccaggact agagttgacc ggttatttta attattattc
     4681 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
     4741 agaagagcgt ttgatcatta tgagaagatt atgtagtgaa gatatgcccg catttattat
     4801 ttcaagagga ttgccggctc cggaagaaat gatccaagca gccaatgaaa atgggatagc
     4861 tgtgctgcgt tcgcctatct caacgtcccg gctgctaggt gaattatcta gttatttaga
     4921 tggacgctta gcccctcgaa caagtgtcca cggtgtgtta gtcgatgtct acggattagg
     4981 tgtactaatt caaggggaca gtgggatagg gaaaagtgaa acggcgttgg aactgattaa
     5041 gcgcggacat cgattgatcg cagacgatcg agtagatgtc tatcagcaag atgaactgac
     5101 ggtaatcgga gaacctccta aaatcctgga acatctgatt gagattcgag ggatcggtat
     5161 catagatgta atgaatcttt tcggtgcgag tgcggtacga ggctctatgc aagtccagtt
     5221 agctgtttat ctagaagcat gggcgaaaga taagaaatac gaccgtctgg gctctgaaga
     5281 cacgaccgtt gaaatcgcgg aagtgggcat tccacaaata aaaatccctg tcaaaacagg
     5341 acgaaacgtt gcaatcatca ttgaagtagc cgcaatgaat ttccgcgcac gtacaatggg
     5401 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
     5461 caatgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
     5521 gtaggaattc ctatctactg gtatgcattg attatcgtat caggaatcat tattgccatg
     5581 tggctcagca gcagagaagc tgttcgggta ggattaaaag cagatgacgt gacagatttt
     5641 atgttgtggg gattgccgat ttccattatt ggtgcccgct tgtattatgt tttattcgat
     5701 cttccccaat atatagctga tccaatccag atattcaata tccgttcagg aggattagcg
     5761 atttacgggg gattgattgc aggtgcaatc acactgtact tttttaccaa atatcatttt
     5821 atttctttgt ggaccttttt agatatcgct gcaccaagcg tattattggc gcaggcaatc
     5881 ggacgatggg gaaatttcat gaaccacgaa gcgtatggtc cagacacgac acgaagtttc
     5941 ttagaaagtc tgcatttacc gcaattcatc attgataaca tgtatatcga aggaatctat
     6001 cgacaaccaa cctttcttta tgaatcactt tggagtttga ttggatttat tctgcttttg
     6061 gttttgcgga agaaaccaca tcttttgaaa aaaggagaag tccttttatg ttatctaatg
     6121 tggtatagtt tcggacgttt ctttatcgaa gggttacgca cagacagcct ttacttttta
     6181 ggtgtgatcc gcatttctca attattgtct gctgttttat ttgtcggcac attgattctg
     6241 tttatttggc gtagaaaaaa aggaagttta ccggattatg accgctcatt aggaaaaaat
     6301 caacaaatta tttgattttc agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
     6361 gaattataga gtagcggttt taggaccagg ctcttggggg actgctttag cacaagtcct
     6421 cgctgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
     6481 caacacccaa cacaccaatc aacgttattt accagatcat catctacccg attccatccg
     6541 agcattcacc gatatggaag atgcagtaaa agatgcagat gcggtactat tcgttatccc
     6601 gacgaaagcg atacgttctg ttgcaaaaga actagttgcc aaattgaata cgaagccagt
     6661 gatcatccac gcaagtaagg gattagaaca gggaacgcat aaaagaatct ctgaaatctt
     6721 gttagaggaa atcccgacag aaaaaagaaa agctgttgct gttctttctg ggccaagcca
     6781 tgcggaagaa gtagcaaagc atgacattac gacgatcaca gcggcaagca caaatgtaaa
     6841 agcagcaaaa tatgtacaag agctgttcat gaaccattat tttcgaatct ataccaaccc
     6901 tgatgtgatt ggtgtagaga tgggagcagc tttgaaaaat attattgcca ttggagctgg
     6961 tgctattcac ggcttagggt ttggcgataa tgcaaaagcg gcaatcatga cgcgcggatt
     7021 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttattggctt
     7081 aagcggagta ggtgatctag tcgttacttg cacaagtgtt cattccagaa actggcgcgc
     7141 aggcaacttg ttaggacaag gtcagcctct agaagaggtg ctagccaata tgggaatggt
     7201 tgttgaaggt gtgaatacta ctcaagcagc gatggaacta gcccaaatga tggaaatcga
     7261 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
     7321 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
     7381 ttgaaggagc gaatgtatat gaaagtaaaa aaagcagtta ttccagcagc aggattagga
     7441 acaagatttt taccagcaac aaaggcgatg gcaaaggaaa tgttgccaat cgtggacaaa
     7501 ccaacgatcc aattcatcgt tgaagaagcg ttgaaatcag gaatcgaaga catcttgatc
     7561 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat cgaattagaa
     7621 acaaacctaa gtgaaaaagg aaaaaccgaa ttattgaaat tagtagaaga aacgacagat
     7681 gtcaatttgc atttcatccg tcaatctcat ccaaaggggt taggacacgc cgttcttcaa
     7741 gcaagagcct ttattggaaa tgaaccgttt gtcgttatgc taggcgatga tatcatggaa
     7801 gacgaagtac cattgacaca gcaattgatc gatgactatg agcgtacaca cgcttctacg
     7861 attgccgtta tgcaagttcc tcacgatgaa acttcaaaat acggaatcat caacccaggg
     7921 gaagtactgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
     7981 aaagcaccaa gtgatttagc tattattggg cgttacttat tgactccaga aatctttgat
     8041 gtattatcag aacaacaacc aggcgctgga aatgagatcc aactaactga cgcaatcgat
     8101 acgttgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttatgacgtt
     8161 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctgacaca tccagaagta
     8221 ggggcacctt tgcgtgaata catcatgtct ttaggtaaaa cattagaagc agaaggaagc
     8281 aagatcaatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataatct
     8341 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
     8401 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
     8461 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
     8521 tttcatcgtt cgcgtattgt tgcaagtttc caaaacttta gaaaaagtag accagactgt
     8581 cgcagaagca aatacgacaa ttgagatcgt cacacaagac gtagatcttt tatctagaca
     8641 agtagaaggc ttgctagtca aaagtaatga gctgctcttg gatatcaatg gaaaagtcgc
     8701 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cggaacttaa
     8761 tcattctagt aaaaacatcg tgacgaaagt cggcggaatc ggaaaaacga cagctaaagc
     8821 gactgttgcc ggaaaagtcg gtggaacagc gatgaaattt ttaaaaacaa aaaacatact
     8881 gaaacgacag gaggaaaata agaatggcta aaaaaggtgg atttttatta ggtgcagtag
     8941 ttggaggtac agccgcagca atcgccgcat tattgttagc acccaaatct ggtaaagaac
     9001 ttcgggatga attggctgct caagcagatg atttgatgga catggcttca gattacacag
     9061 atatggcaaa agaaaaatca tctgagttaa caggtattgc aaaagaaaaa gcgcaagatc
     9121 tttctcaaca agcaggggat ctagcaggca atgttaagca gaaggtaagt gaatcagcag
     9181 aggaagcagg aagcgaatca gatgatatct tgttaaacct caaagaacaa tcagctgatt
     9241 tatctaatcg tttcaaaaaa acagcagaag atctgaaaaa gccagcagac gaattaggta
     9301 gtattgctaa agatacttca gaagatatca tgatcgatgt aaaagatgct gcagataaag
     9361 caggagagac ggtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gacacaaaag
     9421 aaatgttgaa aatggcaaaa gaagatgcaa aagaagatgc aaaagaagct aaaaaagaaa
     9481 tgaaagatac aatgacagat tcgactacca atcactaaga ttactcattt ctagaataaa
     9541 aaaggtgaga gcggaccagc tctcaccttt tttattttca ttactctaaa acaagtaatt
     9601 cttttggtgt agtcgtgaat ggcacacaac catcacttgt cacatacaca caatcttcga
     9661 tacgtacccc tactttttca ggaatataga ttcctggttc aagagagaag cacatccctt
     9721 cttcaatcac taagtcgttt ccttctacaa gagaagggaa ttcgtggacg gttgttccga
     9781 ttccgtgacc taaacggtga ttgaaatatt cgccgtagcc atagctgttg atcacgtttc
     9841 gtgcgatttg atcaagttca cctgctgtca cacctgggcg aactgcctct tgtgcggcaa
     9901 gctgtgcttc aagagtgatg ttatagattt tttcttggaa ttcagttggt ttttgataag
     9961 caactgtacg agtagcatca ctgcaatagc cattccaaat aacgccaaga tcgaataaca
    10021 ctagttcatt tggtgaaacc ttcgtgcttc ctggtgtacc atgagggctt gcagcatttg
    10081 ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac accttgtttt tttaattggt
    10141 attcgatttc tgcgataatt gcttgttcag ttacgccttc ttttactgtt ttgaagccaa
    10201 tttcaaaggc aacatcggcc catttaccag cttcgatcag tttgtctttt tcttctaccg
    10261 ttttgatcag ttgcagacgc tggataatag aagttagatc ggtgtaagaa tctgtacctg
    10321 tcaagatatc agagatagca tcgaagcgat cgacagtcag agcattcttt tcaataccta
    10381 cattaccagg ttgcccatag cgtgcgcgaa gctcttttcc gatgatctcc caaggatttt
    10441 cgctgtctaa gtagccacgt acatcgtacg cccagctgct gttttgagca tcttcaactt
    10501 ccagagcagg agtgaaaagg aaaggatctt gttccagagg gataaaaaga gctagtacac
    10561 gctcgtgagg atcactttca tatccggaaa agtaagcgat gtggccaggg tcgctgatat
    10621 aagccaattc taattggtgt tgtgccatcc attgacgtag ttcattgatt tttacgttat
    10681 tcatttggca acctactttc taaattaatt ttgtattcaa taattgtacc actgattgat
    10741 taaaagacca acgttcttaa aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa
    10801 acggttgcaa ttgttgagat tatttgatat cctattttag aaatgtaaga cgtattttca
    10861 gaaaacatat aaaatatgaa taggagaaca caaatggaaa agcaaacaat cactatctat
    10921 gatgtagcga gagaggcaaa tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat
    10981 ccaaacgtca aaccagcaac aagaaaaaaa gtcttggaag ttatcgatcg cttggattat
    11041 cgtccaaatg cagtcgcacg tggcttggca agtaagaaaa caacaacagt tggggtaatc
    11101 attcctgatg tcagcaatat gttctttgcc tctttagcac gtggtatcga cgatgtggca
    11161 acaatgtaca aatacaatat catcttagca aactcagatg gcaacgatca aaaagaagtc
    11221 aatgtattga ataatttatt ggctaagcaa gtggacggcg tgatctttat gggtcaccat
    11281 atcacagacg aaatccgtgg agaattttca cgttcaaaaa cgcctgttgt actagctggt
    11341 tccattgatc cagatgaaca agtaggtagc gtaaacattg attatgtatc agcaacaaaa
    11401 gatgcagtaa ataaattagc aaaaagcggc aataaaaaaa tcgcttttgt cagcggtgcg
    11461 ctgattgatc ctatcaatgg tcaaaaccgt ctaaaaggct ataaagaagc attaagcgaa
    11521 aacaatctac catatagcga aggattgatt tttgaggcac aatacaactt taaagatggt
    11581 ctgtctctag ttgaccggat ccataatagc ggtgcaacag ctgtctatgt ttcagacgat
    11641 gaattagcca ttggtatctt ggatggttta ttagaccgcg gcgtaaaagt accagaagaa
    11701 ttcgaaatca tcacaagcaa caattcttta ctgacagaag tagctcgtcc tcgtttgaca
    11761 agtatcacac aaccattata cgatttagga gcggtatcca tgcgcctatt gacgaaaatg
    11821 atgaataaag aagaagtcga agaaaaaacg attatcttgc cttatggaat cgaagaaaaa
    11881 ggatcaacta aataataaaa gatcaatcat ttaaaggttg ttcccaaagt ggtcaacttc
    11941 tggaacaacc tttatacgag aaaaggggtt atgacaaaat tttgtcataa cccctttttt
    12001 tgtgacttta aaaaaaagag agaatggcgg aaacaaacct tacgtccatc gtatacgaca
    12061 caatctgtgc gtagatgcta cattttgttc actactatca aaattacttt aaaatgaaaa
    12121 cgtgataaaa aagaaagtag aaaagtagaa aagtaactct aaaaaatcca aaaaaatcca
    12181 ggctcgattg cgtacttatc ttaaaggaga aacaatccat catacataac ccgaaaggag
    12241 acttctattt gcctaagaaa agacgaaaga ccatagtcat cattttgacc attcttacta
    12301 taggaatcgg aggaaaattt tacatggaca aaagagaagc gcaaaaacag caagatttac
    12361 tcgcagtaga aaaacagtcc gtcaaagttc tgaaaaatac ctttgcggat atcaaagaag
    12421 tgaaaatcga acaatttgag aaaaacgaca tgacaggaat ctatgaagca ttgataacga
    12481 tgaccaatac agaaggaaaa gccgtttatt ttgattatag ctttacaaaa gaaagcaatg
    12541 agatagatgg ctatggtgtt gaaaatgaag aagtacaaaa agaagggatt acggttgatg
    12601 agattatagt tatctataca gatgggagtg aggaaaaagt atgacaatta ctgataagca
    12661 gtataaagga ataagtaata aagtatattg gctagatcca aaacatcctc aatatagtaa
    12721 aagttacaaa gaaggcgcca ttaaaaactt tggagaaaca aaattccaaa tcctaaaaat
    12781 caaaaactcc ttcgacggca tgcaggcgat ggctgtagcg ccaatcaaga acggtaaagt
    12841 ggatatttct caagtagtga ttgcttttgc aggaacgaac ttaagcgatt ggaaagattt
    12901 ggaaacggat gcaagaagtg ttatgaatct ctatgggaat agaccaggag caagtttttc
    12961 tgaaaaagca acaacaaaca agattttatc cgttagtgga ttgaaaacag cggggcaaat
    13021 caattcagcg attgcatttg ctaatgaagt aaaaaaggaa tatggagaag ctgctatttc
    13081 tactaccggc cattcattag gtggattctt agcattatat gtagcagcag agaaacaatg
    13141 gaaaaatgtc ggcttcaacg ggccggaccc gtatgatctc ttatcaccag aagcaaaaaa
    13201 atgggtggaa gaaaatcccg gtatgctcac caattaccga aatcacgccg attttatcgg
    13261 taattttggt ggtaatggca caggtgctga agttcggatc agtatggaaa cagcatactt
    13321 gaatctgctt gcttcccaca gtttaacctc ttggaagttt gatgaaaatg gaaagctgat
    13381 tattcctgaa aatgaaaaca atgttaaagc tcgaaaacaa cagaaagaaa accaagcgac
    13441 ccagttgttt tcctatcagt tgcaagaact gaacaacata cgtaaaaaac tacgattaag
    13501 tggcggccgg ctgacagtta atgaacaaat ttacttagat gacacacaag ctaggttagt
    13561 ggtggaaaag ttatcggtaa aaatgaaaag cgctatggaa gaagttatcc gcatcaacca
    13621 aagaggaatt tttgagctag agaaaaaatg gcaagaaggc ttgcggcaag cacgtgttgt
    13681 cgccccttcg ttgactgaac aggaaatcat agaagcatta gctagtgtgg gcgccacaaa
    13741 agagaactta gtcgattctg cgaaagaagt ctatctgcta cgaatcaata aggcaagacg
    13801 tatgggggag gcctttgata cattggcgaa ggagatccaa gacaaaatca atgaaatcgt
    13861 ggcacgagat cgagaattag cacagcaatt caattaaagg agaagtagat gaaactagaa
    13921 gaattggaaa gagattatcg aagtgaacga agacaattag aagctcaaga agatgaattg
    13981 atccacttta agaatcaagg attacgattg atggagcaag aggagcagcg actgcatcat
    14041 gtattaaaga acttagcttt agataatgag cctctgtttc aagcaagacg aagttattcg
    14101 aattatcagg agcattatca acaagcccta caagtagaac gacgaaaact ctatcagcag
    14161 caagacgaac tagaacgttc ctatcgtcaa aaaatcagac aaataactat ggagaaatca
    14221 tcttatgaat gaatttttaa tgcaattact aaaagaatta gagaaaaatc tacatcgatc
    14281 aaatgtacgt tcagttggta tggggatatc tcagcaaact tgggcagaaa aacgattgac
    14341 agatcaaaac caccaaatga ctgcagaaga agctactcaa gaattttcta accgacatgc
    14401 acacttaaaa gaatcagtaa aagggcaatt tgcagaaaaa ctaaccgaat tattccaaag
    14461 acaagaaaag aaattaaaac agtttcgtta gtaaagttat atagaaaaat tttgtgaaaa
    14521 tggtttttca catttttaca taaatttgtc ttaatatcgt agatgtaagc ttttgaacac
    14581 catttattcg gaaaaaggga gctgtgacaa gatttttgtc gcagctccct ttaattctga
    14641 ttaatctatt agatcaatca tctttatcgt ctgattcttt ggtctctgga ttcgctctag
    14701 cataagctga agcggtgttc cagtaatctg tatagttttc atcagtaccg ccaacgccga
    14761 atttaaaggc gcttttcttc ggaccatttt ttgcccaaag tgattccact gtttttcctg
    14821 gcgtttggat agatttttta ttgacagtaa cttttccagc tgtttgtcct gtgaacccag
    14881 atactttttt cttcatcaca tcggatgata agtcaaattt ctcatcagtg ccgaaaatct
    14941 cagggtttgc ttggtaagct tgattgatta gattggcaag ataattagcc gaacgtattc
    15001 ttgcttggct gtccattcca gtattgtcat cgtgtcctgc ccaactactg atagtgatag
    15061 atggagttga gacgatcagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    15121 cccaatctgc atttcctaga tttccgttta agcttgagat gacatctttg aatggtgtag
    15181 tgattttttc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttcgg
    15241 aataaatacg gacagaatta ctttcatgtt tataaatcac attacctgca ttatctgtga
    15301 tggaatcaat gatatagcct tcttcgtaga ctccaccatt tgctaaggcc tggaatccat
    15361 ttgtctgttg aagcgtcgtt acatctactg tccctaaagg agccgattca acgccccaat
    15421 tatcatttgc tgggtagttc atcttcgcca agtagtgctc gtaagcgtat tgtttcgatc
    15481 caccggaatt caacacatct tgatataagt gataagctgg gatattgttg gaccattcca
    15541 gtgactcacg gacggtttgg aaggtgttcg acccttcatt ggtcgcattg acgattttct
    15601 caccagcatt ttttccttct tgccaagtcg ttgcataatc tgatacacgg gattctgatc
    15661 cgacaagtcc catatcgatt gcaggaccgt aaacaagaac cggtttgatg gatgaaccag
    15721 cttgacgttc tgtatcaaag gcgtggttat tttgattctg gctgtaattc cgaccaccca
    15781 cgaagccata gatacgtccg gttctgttat ccatcaatac atttccagtc tccacttggc
    15841 taccagcacc atcatctaac atgtagccat aattttctac gccggtttgc aatgcagcat
    15901 agatatcttt atcaatcgtc gaatggatcg tatatccttt gttttggatc gtttgctgtg
    15961 ctttttgata ataagcttca ctgaccgatg aatctgacat atcagcatta tctttttctg
    16021 ccaattgttg tgcaataatc cttgttgctt cgttcatcac ggtataatac aagaattctc
    16081 tgtcagtctg ttccgcgatt tgttgtggga gaaagtcttt tgtaagatca taggctttcg
    16141 cttcttcgta ttgttctttc gtaatttgtc cttcacggta catactgaat aaaacaaaat
    16201 cttttctggc aagaccggca gaaagatctt cttttagcgc gcctgtattc gtgtatggac
    16261 tatacgtgat agggctttga ggcaaaccag cgatataagc agcttgcggc agagtcacat
    16321 cctttgcatc tacaccaaag attcctctag cagcttcctg gactccagca atattttgcc
    16381 ccttattatt ccgaccaaat ggtgagacat tcaaatacgt agtaatgatc tcatcttttg
    16441 aaaagtgttt ttctacttgt gcagaaagaa cgatctcgtt tgctttacgt ttgaatgttg
    16501 tttcatcggt taggatctgt tgtttgacta attgctgtgt caaagtggat cctccagacg
    16561 agccgatgcc agtcgcttct gaaactagtg ctcgtaaaac agctttaggg acataacctt
    16621 ggtgtttatc gaagtattca tcttctgtac tgataatcgc ggttttcaat aaaggcgaaa
    16681 tcttatcaga tgaaatagtc gtccgcatca gatctgtctg gatttttgat atctctgtat
    16741 tatctgcata aacaatcttt gacgtttccg taatgtttcc tagttctgtc tgcagttctt
    16801 ttttagtcgg caattgtgtg tcttcgacaa gataggcaaa gtagccagca cctattccga
    16861 tacctagagc tgcgaacaac aaaagaagaa cagtaataaa aacagtcaat gattgaaaca
    16921 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    16981 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    17041 caaataatag gacaaaaaaa cagacaaggc tgtttttgac aggaatttct ttttcgaatg
    17101 agataaaacg tgaggaaatg atctttttta tgaaagagtt cttaagcttt tctttaggga
    17161 aagtttcagt tactatggag aacagggcaa agaaaaacaa actattttct ttgtacatca
    17221 tcttatcaat atacaggaat tgttcgaaac aattgccttt caaggaggaa tccatccatg
    17281 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggcgc ggcagaagaa
    17341 gcagccagtt acggatcttc gacctttatg atctatacag gcgctcctca aaatactaga
    17401 agaaaagcta tcgaagagat gaatattgat gccggaaaag catttatggc agaaaacgga
    17461 ctaacaaata tcgtcgttca tgcgccttat atcattaatt tgggaaatac gatcaaacca
    17521 gaaatgtttc catttgctgt acaatttctg agagaagaga ttgtccgtgc ggaagcttta
    17581 ggtgctactc aaatcaccct tcacccaggc gctcatgtag gagcaggggc agaagctggg
    17641 attgctagaa ttattgaggg gttgaatgaa gttttgacaa aagaacagac ggcacagatc
    17701 gctttggaga cgatggcagg gaaaggtacg gaaatcggtc gaacatttga ggaattagcg
    17761 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga tacatgtcat
    17821 acgaatgatg ccggatataa tataaaagaa gatttttcag gtgtaatgga agagtttgat
    17881 aaggtcatcg gattagatcg gcttaaagta ctgcatatca atgattctaa aaatcctcaa
    17941 ggttctcata aagatcggca tgccaatatc ggttttggaa caattggatt tgacacattg
    18001 aacaaaatcg tccatgatcc aatgtttgac aacatttcga aaatattaga aaccccatat
    18061 gtcggtaaag ataaaaagaa agcttacgca ccatatggaa aagaaatttc catgttcaaa
    18121 gcgcaagcat tcgatcccga agtgttccct gagttattag cagaagaagt cacactttaa
    18181 gttttctacc tgacagcaac aaaaaaggat tgggcagtct gccttgtcct tttttgttta
    18241 agaaacaaag gagtgctttt tgcattcgtt ttcgccatgc tccttttgcc ccctcttttt
    18301 aaattcagac aaacagggta tactgataga aaagtaaaaa acgaaaagtt tcacaaacaa
    18361 gtagcaaaac gtgattgaaa ggacgaaaag atgctgaaca ttttaaaccc cttgatcgac
    18421 caactaaaaa agaaacatac atactttatt cttggcatag ccgtattatt tacaattatt
    18481 cggttgtatt tatatagtaa agcaatctat ggaacagata tgtcggcgac ctatgatgat
    18541 cagctgctga ttcactatgc tcaaaatatt ttggatggta aatggctagg ggagtacacg
    18601 actacgacac tttcaaaagg gatttcctac agcttgtttt tagtacttgc acataaactg
    18661 catctttctt atgcagtttt attcggcatg ttcagtattc ttgccagtat attggttgca
    18721 ctagcgttta aaccaatcgt gaagaacaag tatcttcttt taagtatcta tttttatttt
    18781 ttgttttctc cgattagttt tacacatgaa tattccactc gaacatatcg taatgctctc
    18841 gtgatcccga ctgttgagat cattatcgct tgtttactag ctatttatta ccgcaaatat
    18901 aattccagca aaaaattgct gccgtggttt ttagggctag ctgtttcttt tcctttcttt
    18961 tggttcattc gcgaagattc catctggctt cttcctttta ctgtagtcgc gttaggcatc
    19021 agtttcgttc agatcttgct aggacaagac ttttctttca aagaagcaaa gaagttaaaa
    19081 gaacgaccat ttctcctttc gaaacaagaa tggctgaaac tttcattgtt tttactccct
    19141 ctagtcggtt tctccatcgt ctatcagtgg atcgaatata aaaacgagac aacatatggt
    19201 attgcagcca tcaatgaccg ttcgagtggc gaatttggac gtttgatgaa acagctgatc
    19261 cgaatagatg acgaaacaaa cttaaataaa gaaaacagtg agatatgggt ctcgcatgct
    19321 gcattaaata aagctttaga cgcatctcct acatttcata ctgtttctac tcgtattgac
    19381 gaattgtata cgactcatgc gtggacccaa ggcggaaaag taaaagaatt accaggagat
    19441 atcatttttt gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca
    19501 aaagaaacca atgatttttg gaaaaaagtc aatcaagaat tagaagaagc ctatcaaaag
    19561 ggaactatca aaaagaaaaa agaattctat ctgatgggat caggggatgg aaagacgatg
    19621 aatgatctgc caatcgtatt tgattttctg aaatcaggat atgcttatgc atggcgatac
    19681 aaagatttcc atcaaggggg gaattatagt tttgggacgc aagaacaagt cgaacaggca
    19741 caagaattat tgaatcttcc actaatggat aattggatcg atagcagtga tccgagaaca
    19801 cctgatttcc agttgacaaa aacagcaaaa gtcgcgaatg tcatgataaa aatttaccaa
    19861 gttctggcca ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga
    19921 accgtatttg caaaagctga tcgtgcctat tatagttcct ctttgattgt agtcatcgga
    19981 ttaatactga cacaattggt ttttcttttc ggtgtctcgt ggttttgttc ttatgcgcca
    20041 gaaaaaaagg attatttttt aagtgtctac actggcgcag gcgtgccatt aacgcaattc
    20101 atcgcagtaa caagcatgct agtttttagt aaattgaatc tgaaaaaagc tttatcttcc
    20161 aaataaaaag ggaactgtga caaaagtctt gtcgcagttc ctttttccga ataaacggtg
//
LOCUS       DBNIBO010000034        20086 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.037, whole genome shotgun sequence.
ACCESSION   DBNIBO010000034 DBNIBO010000000
VERSION     DBNIBO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.037"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1191
                     /locus_tag="AC1Y77_001086"
     CDS             <1..1191
                     /locus_tag="AC1Y77_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIQ1574678.1"
                     /translation="LLEVQNDKSVEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAP
                     GHNSAPSTSAPSAEAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQV
                     TATGKGGRVTKEDIENFLAGAPSSAPAKSEAPEAATPKEAAPAAESKPAEPAKPFKSN
                     LGDLEERVAMTPTRKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGT
                     KLTFLPYVVKALTATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKD
                     ADRKGMFAIADEINEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVA
                     ILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELL
                     MMEG"
     gene            1199..2605
                     /gene="lpdA"
                     /locus_tag="AC1Y77_001087"
     CDS             1199..2605
                     /gene="lpdA"
                     /locus_tag="AC1Y77_001087"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIQ1574679.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2767..3042
                     /locus_tag="AC1Y77_001088"
     CDS             2767..3042
                     /locus_tag="AC1Y77_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HIQ1574680.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3048..3815
                     /locus_tag="AC1Y77_001089"
     CDS             3048..3815
                     /locus_tag="AC1Y77_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIQ1574681.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3982..5811
                     /gene="typA"
                     /locus_tag="AC1Y77_001090"
     CDS             3982..5811
                     /gene="typA"
                     /locus_tag="AC1Y77_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIQ1574682.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6072..7844
                     /gene="recQ"
                     /locus_tag="AC1Y77_001091"
     CDS             6072..7844
                     /gene="recQ"
                     /locus_tag="AC1Y77_001091"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIQ1574683.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7958..8260
                     /locus_tag="AC1Y77_001092"
     CDS             7958..8260
                     /locus_tag="AC1Y77_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HIQ1574684.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQENYDKKEVGKEE"
     gene            8263..9426
                     /locus_tag="AC1Y77_001093"
     CDS             8263..9426
                     /locus_tag="AC1Y77_001093"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIQ1574685.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKKQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGMTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIILVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9452..12880
                     /locus_tag="AC1Y77_001094"
     CDS             9452..12880
                     /locus_tag="AC1Y77_001094"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HIQ1574686.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12901..14025
                     /locus_tag="AC1Y77_001095"
     CDS             12901..14025
                     /locus_tag="AC1Y77_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HIQ1574687.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14006..14452
                     /locus_tag="AC1Y77_001096"
     CDS             14006..14452
                     /locus_tag="AC1Y77_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HIQ1574688.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14436..14774
                     /locus_tag="AC1Y77_001097"
     CDS             14436..14774
                     /locus_tag="AC1Y77_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HIQ1574689.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14893..15453
                     /gene="rsmD"
                     /locus_tag="AC1Y77_001098"
     CDS             14893..15453
                     /gene="rsmD"
                     /locus_tag="AC1Y77_001098"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIQ1574690.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15450..15941
                     /gene="coaD"
                     /locus_tag="AC1Y77_001099"
     CDS             15450..15941
                     /gene="coaD"
                     /locus_tag="AC1Y77_001099"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIQ1574691.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15931..16974
                     /locus_tag="AC1Y77_001100"
     CDS             15931..16974
                     /locus_tag="AC1Y77_001100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HIQ1574692.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17133..17861
                     /locus_tag="AC1Y77_001101"
     CDS             17133..17861
                     /locus_tag="AC1Y77_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIQ1574693.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17977..18222
                     /locus_tag="AC1Y77_001102"
     CDS             17977..18222
                     /locus_tag="AC1Y77_001102"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIQ1574694.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18219..18437
                     /locus_tag="AC1Y77_001103"
     CDS             18219..18437
                     /locus_tag="AC1Y77_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HIQ1574695.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18573..19112
                     /locus_tag="AC1Y77_001104"
     CDS             18573..19112
                     /locus_tag="AC1Y77_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HIQ1574696.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19105..>20086
                     /locus_tag="AC1Y77_001105"
     CDS             19105..>20086
                     /locus_tag="AC1Y77_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIQ1574697.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPR"
BASE COUNT         6783 a         3455 c         4386 g         5462 t
ORIGIN      
        1 ttactagaag ttcaaaatga caaatcagta gaagaaattc cgtcacctgt cacagggaca
       61 gttaaaaacg tgattgttcc agaaggcact gtagcaaatg ttggggatgt attagtagaa
      121 atcgatgctc ctggacacaa tagtgcaccg tcaactagtg cgccatcagc tgaagcacca
      181 aaagaaaaag tggaaacatc aggttcagca agtgtcgttg aagctgccga tccaaataaa
      241 cgtgtattgg ctatgccatc tgttcgtcaa tttgcccgtg aaaaagatgt agatatctca
      301 caagtaactg caactggaaa aggtggcaga gtaacaaaag aggacatcga aaacttcttg
      361 gcaggagctc caagcagcgc accagcgaaa tcagaagcgc cagaagcggc aactccgaaa
      421 gaagcagctc ctgcagcaga aagcaaaccc gctgaaccag caaaaccatt caagtccaac
      481 ttaggagatt tggaagagcg tgtagcaatg acaccaacac gtaaagctat tgctaaagca
      541 atggtcaata gtaagcatac agctccacac gtgacattgc atgatgaagt agaagtgtct
      601 aaactatggg ataatcggaa acgattcaaa gaagtagctg ctgcaaatgg tacgaaactt
      661 actttcttgc catatgtcgt gaaagcttta acagcgactg ttaagaaata cccagtatta
      721 aatgcatcta ttgacgatgc aaaccaagaa atcgtttata aacattacta caatatcggt
      781 attgcaacag atacggatca cggattatat gtaccaaatg taaaagatgc agaccgcaaa
      841 ggaatgtttg ctattgcaga tgaaatcaat gagaaagcaa aattagctca tgacggcaaa
      901 ctatctgcag aagatatgcg caatggaaca attacgatca gcaatatcgg atctgtaggt
      961 ggcggttggt ttacaccagt catcaactat ccagaagtag ctattttagg tgtaggaacg
     1021 attgcgcaac aaccaatcgt aaacgctgaa ggcgaaatcg ttgtcggtcg tgtgatgaaa
     1081 ctatcactaa gttttgatca ccgtatcgta gatggagcaa ctgcgcaaca agcaatgaac
     1141 aatatcaaac gtcttttagc tgatccagag ctattaatga tggaaggatg ataaacaaat
     1201 ggtagttgga gattttgctg tagaattaga tacagttgta atcggtgcgg gacctggcgg
     1261 ctatgtagct gccatccgtg ctgctgaaat gggacaaaaa gtagcaatca ttgaaagaga
     1321 atatattggc ggtgtttgtt tgaacgtcgg ctgtattcct tcaaaagcat tgatagctgc
     1381 gggacaccat tatcaagagt cactagattc cgaaatgttt ggagtaacta gcgaaaatgt
     1441 caaactagat tttgcgaaaa cacaagaatg gaaagaaaac aaagttgtcc atacgttgac
     1501 ttctggtgtt ggttttctat taaaaaaaca taaggttgaa acaatcgaag gcgaagcgtt
     1561 cttcgtagat gatcacacac tacgcgtcat tcatccagat tcagctcaaa cttattcgtt
     1621 caataacgca atcatcgcaa caggttcccg tccaatcgag attccaggat ttaaattcgg
     1681 cggacgtgtg ttagattcta ctggcggatt agcattgaaa gaagtaccta agaaatttgt
     1741 gatcatcggt ggcggtgtga ttggtgccga attgggcgga gcgtacgcaa acttaggagc
     1801 agaagtaacg attcttgaag gctcaccaca aattttacca acttacgaaa aagatctcgt
     1861 aaaattagta gaagatgatt tcaagaaaaa aggtgtaaca gtcgtaacga atgcgatggc
     1921 aaaagaagca gtcgataacg gtgacagtgt gacagtgaaa tatgcagtgg acgggaaaga
     1981 agaatctgtt actgctgact atgtgatggt cactgtagga cgtcgtccaa atacggatga
     2041 tatgggctta gaacaagcgg gtgtcgaagt aggagaacgt ggattgatca cagtagataa
     2101 acaaggacgt acgaacgtac ctaatatcta tgccatcggt gatatcgttc caggggcggc
     2161 attagcgcat aaagctagtt acgaagcaaa aatcgctgct gaagcaatct ctggcaaaaa
     2221 agtagctgta gattataaag caatgccagc agttgccttt actgatccgg aattggcttc
     2281 tgtaggtatg acaatcaaag aagcaaaaga cgcaggtatc gaagcgaaag catataaatt
     2341 tccattctca gggaacggtc gagcattatc gctaggtaaa acggaaggat tcatccgttt
     2401 agtcacgacg atcgaagata acgtgcttat tggcgcgcaa atcggtggag tcggcgcaag
     2461 tgatatggtc tctgaattag ctttagcaat cgaatcagga atgaatgctg aagatatcgc
     2521 cttgacgatt catcctcatc catctcttgg cgaaatcgtc atggatgctt cggaattagc
     2581 attaggttta ccaatccata tttgatttga ataatacagg cgaaacgcaa ctgaaaaatc
     2641 agttgcgttt tgttttttag aattatcaat aatattatta tgtaaactta aaaatatgac
     2701 aacttcttgg tttattacca cttcctatgc tacactgaag aggaatatga aataggagag
     2761 ttgaagatgc aagaatatca gtatccactt gatttagatt ggacaacaga cgaaatggtc
     2821 attgtaatga agatgtggga agatctggaa cgtgcgaatg aaaaagggat aaacaatgaa
     2881 gcttttttga agacttatca gcagtttaaa actgttgtca aatccattgg tgaggaaaaa
     2941 cgattgggac gggaatttga aaaagcttcc ggttattctt tatatcgtac agtgcagcaa
     3001 gcaaaaaaaa atccaaacaa aactttgaga atgaacgggt gagataaatg gaacagatga
     3061 ttgatcatat caagtcctgg cttgaagaag ctggtgagaa aatcaaatct tctcaacaaa
     3121 cattaacaat ctcgcaaaaa tcaaatcgaa cggatctagt aacaaatgtc gatcgaaaca
     3181 tccagaattt tctaatcgaa aaaatccatt cctttgatcc agaggcgaaa atattggcag
     3241 aagaagaagg gtgtaatcaa ttatcggatc tcgatggaag agtctttatc atcgatccta
     3301 ttgatggcac cttgaatttt gtgattgaag gggaaaattt ttgtattatg ctcgctgttt
     3361 atgaatccgg cgttggaaag cttggtttca tttatgacgt gatgagaaat gaactttttt
     3421 ggggtggaaa aggaataggc gtgtataaaa atgatgagcc attgcctaga ccagcaaata
     3481 aatccttatc ggaaggctta cttggtgtga atagttatct tttcggtcat aataaatatg
     3541 gaatccgaga aattgcggaa agcagcatgg ggacacgtat gtacggatgc gctggtcttg
     3601 aactagtggc aatcttgaaa ggaaaccaca ttgcttatat ttcaaacctt agcccttggg
     3661 attatgcagc gggaaatgtc ttattggaag agtttggtat ggtaacaagc ggtctttttg
     3721 gtactccttt gacatttgaa aaaagggaat actatttagc agggacttct tgtgcatatg
     3781 cagaaatcgc agaccttttg aaaaaagaag gctgaaaaaa ggtagaaatc attcacaaaa
     3841 atgataaaaa cgggcaaaag attaattttc tcgaaaaaat gaaaacattt tactatattt
     3901 catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca taaagaacca
     3961 ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt cgcaattatt
     4021 gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca atcagataca
     4081 ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct agaaaaagaa
     4141 cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggaat tcgcgtaaac
     4201 atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat catgaaaatg
     4261 gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca aacacgtttt
     4321 gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa caagattgac
     4381 aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt catcgaatta
     4441 ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact aaatggtacg
     4501 tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat ttttgataca
     4561 attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca attccaagtt
     4621 tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt atttagagga
     4681 acaatcaaag taggagacca agtagcatta attaaattag acggaacagt taagaaattt
     4741 cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca agaagcaaaa
     4801 gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga aacagttact
     4861 cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac attgcaaatg
     4921 actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt
     4981 aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg agttgaacca
     5041 acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt gtctatcttg
     5101 attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga agttattgaa
     5161 aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa
     5221 gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat gcaagatatg
     5281 gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg aggtttgatt
     5341 ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa ccatactttt
     5401 gatcaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt
     5461 tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga acgcggtaca
     5521 gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga aaacagccgt
     5581 gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct
     5641 actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga agaatctttg
     5701 gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg cttgagaaaa
     5761 caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa
     5821 ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt tgtccaactc
     5881 ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac gccaaaagac
     5941 gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa caagataatg
     6001 tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa
     6061 ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag tttagacctg
     6121 gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga atcatgccta
     6181 ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat gggcttactg
     6241 tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg aatcaattag
     6301 gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct agacgcttcc
     6361 aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt tttatcatgc
     6421 cagattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt gacgaggcac
     6481 attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg gcgaaccagc
     6541 tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg actgtgcaag
     6601 tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt cagactggtt
     6661 ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa
     6721 tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca acgagaaaag
     6781 aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt agatatcatg
     6841 gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc
     6901 agctgattgt agcgaccaat gcattcggca tgggtataaa taaaagcaat gtccgttttg
     6961 ttatccatta tcaaattcct ggctctctag aggcatacta tcaggaagct ggtcgggcgg
     7021 gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat atccaagtcc
     7081 aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa
     7141 aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa
     7201 actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta gatgacagag
     7261 aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg
     7321 agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc
     7381 aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa tcgcaaaaag
     7441 aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc atcaatggtg
     7501 agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga caagaatctg
     7561 tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc gatacactat
     7621 ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt cccccgtatg
     7681 tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca agcagattgg
     7741 agatgcttca gataaaaggg gttggccaaa gcaagcttga taaatatggc gaagcctttt
     7801 tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa cgaatacgta
     7861 aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa aatcaaatat
     7921 gttataatta aggaattaga atgggaggta agcacagatg gattctttgt cgaaaaaact
     7981 tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa
     8041 caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata cacagatgta
     8101 cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa agaacgaggg
     8161 ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg aggtttacca
     8221 agaaaattat gataaaaaag aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa
     8281 aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg tgtattggga
     8341 ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca aaatccgtcc
     8401 gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat cgcactactt
     8461 tataaaatga aaacagatgt tttgaaaaaa cagcgtttga tcatggcagc catcgcagtt
     8521 ttgacgattt tgctgctgat cgttgtgttt ttcggtaaag aaatcaatgg tgcaaaaggt
     8581 tggcttcaaa ttgcaggatt ttcgatccaa cctgccgaat acctaaagat catttctatc
     8641 tggtatctgt ctctgacttt atcaaaaagg caaaacagtg tacaaaaaga tttcctaggg
     8701 actgtaaaac gtccacttgc aatggttatc ggaatgactg ctttggtcgc tatccttccc
     8761 gattttggga atgcggcagt tatctttttg attattttgg ttctgctttt agccagcgga
     8821 gtcaattatg tgtatacttt gattgtagga gtagggggtt tttgtttaag tacttttacg
     8881 atctggctaa tcaatattac aaacggaaaa atcttgcctg gacgtttaca gtatatctat
     8941 aatcgctttg ctatttacca aaatcctttc tcggatgaat taaataaagg ccatcaatta
     9001 gttaatggtt actatgccat gttcaatggc gggttatttg gcagaggatt aggtaacagt
     9061 atccagaaaa agggattttt acaagaagca caaaccgatt ttatctatgc gatcgtagtg
     9121 gaagagctcg gagtgattat ggggattttg atattagcgt tgctattctt tatgattgtg
     9181 cgcattattc ttgtagggat tcgttcaaaa gatccgttca attcgctttt gtgcattggg
     9241 atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac tggcgttatt
     9301 cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt gctgatgctt
     9361 tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt
     9421 ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg ctaacagggg
     9481 tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat
     9541 ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag catatttagt
     9601 aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca tttctatcgc
     9661 gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg aaaacttgaa
     9721 ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac tgcatcattt
     9781 agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc
     9841 gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt ttgcgaaaca
     9901 atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt
     9961 agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc
    10021 agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta agcatatcga
    10081 agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa gagattgttc
    10141 tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca
    10201 gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa
    10261 cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg aagtcaaccc
    10321 tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata tcgtaacaac
    10381 gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc ctcagcaaga
    10441 aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag acccgttgaa
    10501 taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt
    10561 acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt ttgattcatt
    10621 gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga aaatggaacg
    10681 ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta tgctcaatgt
    10741 catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg atagtacagc
    10801 aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga aatatatcgg
    10861 tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga
    10921 accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta agaatgtatt
    10981 agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa atttgttgtt
    11041 gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg ttcggacaaa
    11101 ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct tttcaagtga
    11161 gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag atccttggca
    11221 acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg
    11281 ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga
    11341 agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact ggactccaca
    11401 aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg cagctatttg
    11461 ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt attataaaga
    11521 catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag atatggctgg
    11581 gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa cgacagatct
    11641 gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt attctgctgc
    11701 ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag
    11761 tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc tgccagaaat
    11821 cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca tgtattacaa
    11881 accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg aaatgccagg
    11941 aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc atcgctggga
    12001 tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac
    12061 tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg atcttacaga
    12121 agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg tgactttctt
    12181 ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa agattattct
    12241 taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa
    12301 cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt
    12361 aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata atcaatttgg
    12421 cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg gtgaaacaat
    12481 caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc
    12541 cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac gtgaaatcac
    12601 tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa
    12661 ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg
    12721 cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa tggaaacggc
    12781 catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag gagacactat
    12841 ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag gtggggaaac
    12901 atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc attctacatg
    12961 gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca acaaatatca
    13021 aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc tacttatatc
    13081 ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg attaactagc
    13141 ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac atttgaagcg
    13201 aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc tgctgatatc
    13261 gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac tatctatact
    13321 aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga agaggatatg
    13381 aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt ttttgatcaa
    13441 acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt gaaactattg
    13501 ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc ggctgattgg
    13561 gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat gcttagaaga
    13621 gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag tcagctgcta
    13681 ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat ggctgattgg
    13741 caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa tgatgaattg
    13801 gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat tcatccggcg
    13861 attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca tgaccgtttc
    13921 atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat gttagtctta
    13981 ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag agctgtttca
    14041 actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa cgatggcttc
    14101 ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt tgaaagaagc
    14161 gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt atgcacctga
    14221 ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata tggatgagca
    14281 tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca
    14341 agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc cgttttttga
    14401 aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat agtgaatttg
    14461 aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc
    14521 tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc gtgctttact
    14581 taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat tttgttcgaa
    14641 acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca
    14701 aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa atccgattag
    14761 ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac ttggataacg
    14821 atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt accatttcta
    14881 aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt aaaaagttta
    14941 gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt caatatgatt
    15001 ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg tggtttagct
    15061 atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg
    15121 ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt taccacaata
    15181 aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca attcgactac
    15241 ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa
    15301 gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa
    15361 ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg cataacggcc
    15421 gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca ggaagttttg
    15481 atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg
    15541 tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca gaagaaagaa
    15601 tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa
    15661 atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac
    15721 gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat catctatatg
    15781 atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg agttccagta
    15841 ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg ccagtgatca
    15901 acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta
    15961 ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt gccgtattat
    16021 gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa tgataaggaa
    16081 gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc tacaggtttc
    16141 ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga agaattgact
    16201 ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga ttcttcccaa
    16261 aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat ggaattcaaa
    16321 ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct ttcagttgga
    16381 gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt tatggattat
    16441 gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa tggtgaagaa
    16501 aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg aataggtatc
    16561 acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa ttctggctct
    16621 atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca agtcactggt
    16681 aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag tgaaggagaa
    16741 gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc tggcgtagag
    16801 attttctttg caccagatga tacgatttca gacgatgtta agaaagaata tcctgaaata
    16861 aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc
    16921 gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa atgaaactaa
    16981 ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc gtcaaaaatc
    17041 ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg aagggacgat
    17101 tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat gatttttact
    17161 ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata ctagcttcac
    17221 ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca agtggttttt
    17281 tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa gaaatacttt
    17341 cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta gaagaggatg
    17401 ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct ggtatctatt
    17461 ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga ttattagaag
    17521 aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa gtcctatata
    17581 tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc tcggaaggaa
    17641 aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc actccatcag
    17701 aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt cgatttagag
    17761 aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt atcggtgaaa
    17821 agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca aaatcgactg
    17881 gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta agagcttggc
    17941 aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa agaaacatta
    18001 aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca tattatgaat
    18061 acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa agatgtactt
    18121 agctatacag cagataaagt atttattctc attgggacga atgatctagc agagcataaa
    18181 caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag
    18241 actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag acaaaacaaa
    18301 aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa gtagcaaaac
    18361 gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg attcgaagaa
    18421 tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt aggatctttt
    18481 gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg agtacttttt
    18541 aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca aaagcatgcg
    18601 atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct tgggaccagt
    18661 actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt ttagaagtag
    18721 gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga gcagtcagtg
    18781 gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga
    18841 tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg acggaaggtt
    18901 cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg ttgcaagctg
    18961 gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc caagcgttga
    19021 tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat ttttcaaaac
    19081 ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg
    19141 ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac tgacaattgg
    19201 ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga aggacgtttt
    19261 atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag gctacaagag
    19321 tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac agatacgatt
    19381 cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa acaaaagctc
    19441 cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa cttccgagga
    19501 aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt
    19561 tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg gttccaaatt
    19621 caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca gagaaaaaga
    19681 ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt atatattaat
    19741 tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat ttttagggag
    19801 agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta tttagggtgg
    19861 ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat tccgttgagt
    19921 ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt gcgtgctagt
    19981 ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc caccatggac
    20041 ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgat
//
LOCUS       DBNIBO010000035        19622 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.038, whole genome shotgun sequence.
ACCESSION   DBNIBO010000035 DBNIBO010000000
VERSION     DBNIBO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.038"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(287..649)
                     /locus_tag="AC1Y77_001106"
     CDS             complement(287..649)
                     /locus_tag="AC1Y77_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574698.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="AC1Y77_001107"
     CDS             complement(723..1103)
                     /locus_tag="AC1Y77_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HIQ1574699.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="AC1Y77_001108"
     CDS             1185..2360
                     /locus_tag="AC1Y77_001108"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIQ1574700.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="AC1Y77_001109"
     CDS             complement(2437..3021)
                     /locus_tag="AC1Y77_001109"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1574701.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="AC1Y77_001110"
     CDS             3222..4220
                     /locus_tag="AC1Y77_001110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1574702.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="AC1Y77_001111"
     CDS             4332..4841
                     /locus_tag="AC1Y77_001111"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIQ1574703.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="AC1Y77_001112"
     CDS             complement(4871..5530)
                     /locus_tag="AC1Y77_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIQ1574704.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="AC1Y77_001113"
     CDS             complement(5532..6296)
                     /locus_tag="AC1Y77_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HIQ1574705.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="AC1Y77_001114"
     CDS             complement(6304..6981)
                     /locus_tag="AC1Y77_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HIQ1574706.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="AC1Y77_001115"
     CDS             complement(7018..8403)
                     /locus_tag="AC1Y77_001115"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIQ1574707.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8612..8698)
                     /gene="ffs"
                     /locus_tag="AC1Y77_001116"
     ncRNA           complement(8612..8698)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC1Y77_001116"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="AC1Y77_001117"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="AC1Y77_001117"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIQ1574708.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="AC1Y77_001118"
     CDS             9342..10199
                     /locus_tag="AC1Y77_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574709.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="AC1Y77_001119"
     CDS             complement(10280..10615)
                     /locus_tag="AC1Y77_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574710.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="AC1Y77_001120"
     CDS             complement(10682..12052)
                     /locus_tag="AC1Y77_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIQ1574711.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="AC1Y77_001121"
     CDS             complement(12130..12666)
                     /locus_tag="AC1Y77_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1574712.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="AC1Y77_001122"
     CDS             12975..14456
                     /locus_tag="AC1Y77_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574713.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="AC1Y77_001123"
     CDS             complement(14559..15266)
                     /locus_tag="AC1Y77_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIQ1574714.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="AC1Y77_001124"
     CDS             complement(15345..15683)
                     /locus_tag="AC1Y77_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HIQ1574715.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="AC1Y77_001125"
     CDS             complement(15794..16756)
                     /locus_tag="AC1Y77_001125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIQ1574716.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="AC1Y77_001126"
     CDS             complement(16771..18102)
                     /locus_tag="AC1Y77_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HIQ1574717.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="AC1Y77_001127"
     CDS             complement(18110..18790)
                     /locus_tag="AC1Y77_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIQ1574718.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="AC1Y77_001128"
     CDS             complement(19009..19488)
                     /locus_tag="AC1Y77_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIQ1574719.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         5850 a         4034 c         3248 g         6490 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tt
//
LOCUS       DBNIBO010000036        19452 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.039, whole genome shotgun sequence.
ACCESSION   DBNIBO010000036 DBNIBO010000000
VERSION     DBNIBO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.039"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            133..3660
                     /locus_tag="AC1Y77_001129"
     CDS             133..3660
                     /locus_tag="AC1Y77_001129"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIQ1574720.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="AC1Y77_001130"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="AC1Y77_001130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIQ1574721.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="AC1Y77_001131"
     CDS             7448..7789
                     /locus_tag="AC1Y77_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIQ1574722.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     misc_binding    7833..8044
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="AC1Y77_001132"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="AC1Y77_001132"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIQ1574723.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="AC1Y77_001133"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="AC1Y77_001133"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIQ1574724.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="AC1Y77_001134"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="AC1Y77_001134"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIQ1574725.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="AC1Y77_001135"
     CDS             13000..13737
                     /locus_tag="AC1Y77_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574726.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="AC1Y77_001136"
     CDS             13751..14566
                     /locus_tag="AC1Y77_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIQ1574727.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="AC1Y77_001137"
     CDS             14579..15226
                     /locus_tag="AC1Y77_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1574728.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="AC1Y77_001138"
     CDS             15242..15898
                     /locus_tag="AC1Y77_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1574729.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="AC1Y77_001139"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="AC1Y77_001139"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIQ1574730.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="AC1Y77_001140"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="AC1Y77_001140"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIQ1574731.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="AC1Y77_001141"
     CDS             18240..18758
                     /locus_tag="AC1Y77_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1574732.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="AC1Y77_001142"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="AC1Y77_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HIQ1574733.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DBNIBO010000037        18974 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.040, whole genome shotgun sequence.
ACCESSION   DBNIBO010000037 DBNIBO010000000
VERSION     DBNIBO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.040"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(361..987)
                     /locus_tag="AC1Y77_001143"
     CDS             complement(361..987)
                     /locus_tag="AC1Y77_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574734.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1282..1617)
                     /locus_tag="AC1Y77_001144"
     CDS             complement(1282..1617)
                     /locus_tag="AC1Y77_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HIQ1574735.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1604..1888)
                     /locus_tag="AC1Y77_001145"
     CDS             complement(1604..1888)
                     /locus_tag="AC1Y77_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIQ1574736.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1944..3467)
                     /locus_tag="AC1Y77_001146"
     CDS             complement(1944..3467)
                     /locus_tag="AC1Y77_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIQ1574737.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3460..4635)
                     /locus_tag="AC1Y77_001147"
     CDS             complement(3460..4635)
                     /locus_tag="AC1Y77_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIQ1574738.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4639..4824)
                     /locus_tag="AC1Y77_001148"
     CDS             complement(4639..4824)
                     /locus_tag="AC1Y77_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574739.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4790..6484)
                     /locus_tag="AC1Y77_001149"
     CDS             complement(4790..6484)
                     /locus_tag="AC1Y77_001149"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HIQ1574740.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6481..6954)
                     /locus_tag="AC1Y77_001150"
     CDS             complement(6481..6954)
                     /locus_tag="AC1Y77_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HIQ1574741.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7023..7178)
                     /locus_tag="AC1Y77_001151"
     CDS             complement(7023..7178)
                     /locus_tag="AC1Y77_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HIQ1574742.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7312..7692)
                     /locus_tag="AC1Y77_001152"
     CDS             complement(7312..7692)
                     /locus_tag="AC1Y77_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIQ1574743.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7696..7911)
                     /locus_tag="AC1Y77_001153"
     CDS             complement(7696..7911)
                     /locus_tag="AC1Y77_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574744.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7914..8321)
                     /locus_tag="AC1Y77_001154"
     CDS             complement(7914..8321)
                     /locus_tag="AC1Y77_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HIQ1574745.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8587..10062)
                     /locus_tag="AC1Y77_001155"
     CDS             complement(8587..10062)
                     /locus_tag="AC1Y77_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HIQ1574746.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRDVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10052..10900)
                     /locus_tag="AC1Y77_001156"
     CDS             complement(10052..10900)
                     /locus_tag="AC1Y77_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HIQ1574747.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10937..11299)
                     /locus_tag="AC1Y77_001157"
     CDS             complement(10937..11299)
                     /locus_tag="AC1Y77_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574748.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11300..11458)
                     /locus_tag="AC1Y77_001158"
     CDS             complement(11300..11458)
                     /locus_tag="AC1Y77_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574749.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11599..12223)
                     /locus_tag="AC1Y77_001159"
                     /pseudo
     CDS             complement(11599..12223)
                     /locus_tag="AC1Y77_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12220..12531)
                     /locus_tag="AC1Y77_001160"
     CDS             complement(12220..12531)
                     /locus_tag="AC1Y77_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574750.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12575..12868)
                     /locus_tag="AC1Y77_001161"
     CDS             complement(12575..12868)
                     /locus_tag="AC1Y77_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574751.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13062..13709
                     /locus_tag="AC1Y77_001162"
     CDS             13062..13709
                     /locus_tag="AC1Y77_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574752.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13770..14915
                     /locus_tag="AC1Y77_001163"
     CDS             13770..14915
                     /locus_tag="AC1Y77_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1574753.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15354..16172)
                     /locus_tag="AC1Y77_001164"
     CDS             complement(15354..16172)
                     /locus_tag="AC1Y77_001164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574754.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(16272..17597)
                     /locus_tag="AC1Y77_001165"
     CDS             complement(16272..17597)
                     /locus_tag="AC1Y77_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574755.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(17766..18695)
                     /locus_tag="AC1Y77_001166"
     CDS             complement(17766..18695)
                     /locus_tag="AC1Y77_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574756.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIH
                     QQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVIT
                     EIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRK
                     DNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
BASE COUNT         5574 a         3785 c         2866 g         6749 t
ORIGIN      
        1 ctgtgaagtc aacgttttga cctgattatt agagaaacaa atggcaaaat agttcttcat
       61 atttcgccgt ttttcgagcg aatgccgagg ggcagctgat tgaacatcgt ttatttggaa
      121 aaagggattg tgacaagacg tttgtcacaa tccctttttt tatatcttat taattcattc
      181 ttttgttcta ttcttcttct tccgccatga atgagaaacc catatcaaag aactataaag
      241 gttatcaaaa cagcgtttat gactaaatga attagagtta tctatcacta tttttcaagc
      301 gatttaaacc aaatggcata caatatggca tacaaaaact tcaataagcc gtgggggttt
      361 ttaaatttct tcttgttctt taaaatcaat gagtggagct acataatttg aatccacttt
      421 ttcaaacgtc actttttgca tcgacgaatg ttttctttcc caagataacg taaatagatt
      481 tgactctgga cttataaagt ctacttcacc ttctagtatt actttcttat gcaaatattc
      541 ctctaataaa tatagacttg ctaaagcata caacacattt tccaaatttg cttccttata
      601 atttgaagaa cgaccatgtt ttactctgtt atatattttc caccaatctg gtgtatcttc
      661 aggccaccca tctccaaaag gttttagtga agtcaatgtt aaagaattca taaccctaac
      721 accattatcc aaacacatta attcgctaga tttaataaca gctttaaaat cagttatgct
      781 tttattgcta ctgctatcaa atccgcaaag ttctttgaaa ataatctcac actccgaacc
      841 aatcgctagt agtagttttt gatattcaat agagaatgtt ttgaaattat tctcatcgag
      901 catcacaaaa tctgtggtat taataaactc attttctaaa tgtagataat acttccaata
      961 aaaatttgta aactctgctt tgtccattaa atcacctctt ttgaattata acaaaaaaaa
     1021 cacgtccaca acagacgtgt tctttgattt ctacacaatc agcttgccaa aaccatccaa
     1081 tcagtcctag catgcctgta ttaaaattga acgttcatca tctatcatct ttcttagtcc
     1141 agcatgtttg caaccacacc tacaattttc agtccaacaa tagatttcga ctagctaagt
     1201 cttgcaaaat tcaggcagct aacccttcat ttactacctt aattatacta aatacccaca
     1261 acgtttacaa gtgtaatcat tctaagaaat ctgtttagct atgatcgttg tataatcttt
     1321 aaacgccgtt ccttcattga ttgaaataat tttataactc ttgtttttcc aagctacttt
     1381 catatcgttt gttagttcat aatcttgatc gtatcgaata ataaacgtca acgttccttc
     1441 aagaatggta cctacactag caagcacgtc ttttagcatt tgtgaacgca cattagccca
     1501 acattcaaat acagtttctt cactatccac catctgccca tcttcatctt ttacccgttt
     1561 tgtcttttta aacttgactc tctcagtcaa ttcatttaca ttcttaatga ttggcataat
     1621 aagcacctct catttgttgt actaatgctt gtaccgttac tggtatccgt tcgctgctgg
     1681 cttcttgacg gttcaaatac caatgttgag taagtaatga caccgccaaa tcaaattgct
     1741 gataattatc catatcttta tccgttgcgc tactatcaat agcacttttt atatattcgc
     1801 ctgctgtatc aatcaattgt tgaataaagt catcatcaat agtgtgatcg atccgcatac
     1861 ttttttttat gctatccaaa attaccattt tatctctcct aatgaaaaaa aggcagtggg
     1921 ataaaccgcc gcccaatatt tttctatact tttggtgtta caactggcgt aaactcaata
     1981 tacactgaag cattttgatc aattttctta taatcgttgc gaacgatcac tgcaagtcct
     2041 tgtgagtagt aatcgaattt ttcccattga gtcgttactt gatttctacg agcaacaaag
     2101 atagattgtg caatatcccc cataatcata gggaatgttc cagctttagg attggcaaac
     2161 aatgtatctg caatcaatac tactggcata ccgaataatg atttaccact aggtgctgtc
     2221 acatcgggtt gtaaaatgta acgtccatct gaatctttca atgtatctag gtggtaatat
     2281 ccgctttggt ttagaattac cattttattt aatgctgggt ctaatgccac attgtatagt
     2341 tgtttcaaat cgtccaacgt agcagccgtt ttcttagtga atgtttttaa taaatccatg
     2401 atatgcttat tgtcagtgtt ttctaccaat ttcgctaact gatctttgac ctcttgtaca
     2461 atattcactg ctgaatcttc cacaacctca tttgataagg caatcttacc agcacgagtt
     2521 tctactttat aatcaactga agtaaacatt tcagcgtcaa tatcaccaat ttcagcaagt
     2581 tcagcttttg ttgctaacac tgcttgttgg ttagttgcta ctggatattt accttgtcca
     2641 tttgatactg tttttactgt tgcatattga gctaagttat aatttgaacg tttcaaatca
     2701 aagacttcac cgattacttc ttcgggaaca actgccgctg catttacagt agtgactcca
     2761 tcacgcactt cgccttgtga acggatatat tcttcatacc cacgcaattc tgttttttcg
     2821 ttatcgatta aagttttttc catgttatta ctctcctttt gtgctttaga agtggattct
     2881 aagctattgt ttgtttgttt tgcttggttg ttactcataa acgattcgta agaacgctta
     2941 tttacttgga catttgatga atcataagcg ggaacggtca cgacactaat ttcattaagt
     3001 gctttgattt tatctattga tcgttcaatt gtgccatctt cttttttgtc gaatgaatct
     3061 aatccaagca cgaacccaaa ggacatagaa tcaacaaccc cttttgagat attctcatac
     3121 acatcatttg aataggtcgt gtcggttagc tctgcttcaa aatgtaatcc tgtatcatcg
     3181 acattcaatt tcaacgtacc tgctttgaca ctagctagtg gctttgaata atcgtgtcca
     3241 tataataaga acacatttga taaatccacc tcttttaatg cttcgggtgt aatcacttct
     3301 acaaagccgc ctaaatcttt tgacggttgc ccgaatttca acgcatagcc tgaaatagtt
     3361 ttgccgtctg ttggttgttt ttcattttct ttctctactt tgggagaatc ggctgttagc
     3421 tcagcttctt ctgtcagccg tttttccttt tcttcttcat tattcaactg gtgtaactcc
     3481 ttcctgttct tttaattgat atttctccaa cgtatctaaa tacgtaaagt tcaaacttgc
     3541 tagtaaacga tcgccaccat ccatgggggg tagccccatt ttcgaccgcc cctcattaat
     3601 cgtgagaagt gaccctttga cctgatccaa tacatttttt acttttgttt caggatcagt
     3661 ctctagtaac tgatcggaat taaagcggat attggtttca agtttcgtat ctaattcact
     3721 cacaaacaca ttaaagtaat gaatcagcgt actttggata tactggagat tactttgaac
     3781 ggtgctagaa tgttcatttt caacgcctaa acgctcaatc ggtacaccaa acgccttggc
     3841 aatttgtttt gtgttccaat cattggaatt gataagcttc aaaacatcgg tgtttatttc
     3901 taatgttttg tagtccattg tttcatcaag aatgatcgtt ctaagagcat tatctccact
     3961 acttgaaccg ttggcttctt caaacttttc acgaattgcg ttctttgctg atccgtctaa
     4021 atctgactta tgaaccttca aaatacctga cccactgaca ccacgagtaa agaaattgtg
     4081 caatgtgcgg ttgccagctt gttgaatttt cagttcatca cgtaacgcat aaagtggtgg
     4141 taatcctgtt aaaccatctt gggtgaaata tttgaagtgc aaaatatcgc tagacttcac
     4201 acgtcttttt ttatcaccaa tctgataaga caacgtacca ttatctaatt gagtaaccgt
     4261 tacacttgag ttaggtaata agtgaatttc ttctaccttt tcaccgttgc gtttaatctc
     4321 tgcaaagcta ttaccgttta gcaacatatt gaccgccaaa gcaaacttta aatgccaacc
     4381 atccatttct gaattaggtt tctcgtttag taacttcacc agttcattat cagcttgtgg
     4441 catattgttt ttgaccaatt gaatcggact tgaagcaata tcactagcaa taattcgaac
     4501 ggctgcaaac acatcactat ttcttaatgc acgaacgctt gtaaagctgg tggtgtaatc
     4561 atccgattgg attgatacca catgttgtag gaaaggatct cccgttgtat tttgtctcgc
     4621 tttaaaaaat gccatctttt aacctccctt ctgttctgta tttagcatga ccgctacccc
     4681 aataagcact atgcccagcg ctaacaagcc atagtagaca tttgttagta aggtgatagc
     4741 aaccaacaca cacaccaatc caagaagtaa aagaacggta tgaatgtaat tagaaagaaa
     4801 attcttcact tgtataaaat tcattgtctg ctttcgctcc ttccgttgtg gtgaagtaat
     4861 ccattgcaaa cacataggca ttgattaatg cagctattgg atcaattttg ttactgttct
     4921 ttgatttatt aatctgaatg ccgttgttat ccgtttttaa aatagcgttg ttcactgcat
     4981 gggtgagaat cgtattttta ttgtgaataa cgttgccttc ataaacttgt tctctaaaag
     5041 ttcgggtcgg aacgtttaga gtaatcgttc cttgtcttac ttctaacatt gggtagttgg
     5101 ctttttcagc tttactgata agcgaattag cgttatacgg gtcataacaa atccccatac
     5161 attccaaatc attttcttga atcaaatctt gaataaactg aaatacttgg tcatagtcca
     5221 cgattccact ttctaattga gtaatggaac actcaccagc tttttctagt tctcgataag
     5281 gcaaaccgtc gcgcttttct ttgtcttgaa gcccatattt ggtggctaca aagctatgtg
     5341 aatcacaata aagttcgccg ttatctagcg gtacgatcca tgaaacgcta gttaaatcat
     5401 ctgttttaga taaatccact ccgatataaa cgggcttgcc tgtaatatct tgtggttcta
     5461 cttcggttgc ctgccagtct tcagcaatca tatagctgtc ctcactcgct tgtctccata
     5521 aattgaagtt tttcaccaat acagaattaa ggttattttg tttcaaagca aggttcacat
     5581 catcttgaat ggttggaatc attacttttt tgatttcttc actttcaaaa atcggattgg
     5641 ctttgatcca tttctcttga tcgtgaattt cttcgggatc gtccaattcc catatcgcta
     5701 taaagtacct gtcggcttgt tcttcgcctt ttagcacacg atctaacaat aagtattcct
     5761 cgtacatagg aacgtttaaa tcaagcccag cggtactaat aacacacaat aagccgttct
     5821 tttgttgggt cattcctgac ttaatgacgt tgtacgtttt acgagttttt gcttcgtgcc
     5881 actcgtcaag aattgcggtt gttggtgcaa acccatctag cgtgctggtt tcactagcca
     5941 gtgccatagc gaacgaatta gagggcaagt gctgaatctt agagttcata attttcagtt
     6001 gtggtcttaa aaacttacta gacttgacca cgtttctaag tgaattagac agcatatcat
     6061 agcccaattt agcttgtttc aaagcgttac ttacaaacaa tacttgcctt gcttctgctg
     6121 gttcttgttc cataatcaac gcattggcag ccatgccact tgcgatatac gttttcccat
     6181 tcttacggct catactggta aacgctcgat tgaatcgcct gtaattccct tctttagtac
     6241 gccagccata aagactgcct agaatgaatt tttgaaatcc taacatcttg attgctttac
     6301 cgtccgtaga tggtagcaac tcaatgaatt taatcgcttt ggtggcttgt tcttcatcga
     6361 acacataagg gaagctatct gacttagaac gctccaaatc gcttatatga cgctcacacg
     6421 cttggataat tttcttgggt gcaatcagct tacctgatag gactttctca atgtaatcat
     6481 tcatgaattc atcaactctt tgaacgggtc ttcgggttca tcatttttaa ccttgttcaa
     6541 agctagtttt gatcgtgatt ctaaggtcat acctagtgag gtggcttgtt ttttcaaatc
     6601 actcatagct tgtgattgaa cccgaaccgc tggattggct ttctttactc cgttttcgca
     6661 tgtgataaac gtacctgatt ttcttatctc ggcttcggct gttttcattc gagcaaaggc
     6721 taaacaatag gctaccaatg aaccataatc tgcttcactc aatggataat ctttttttaa
     6781 tgttggtacg atacgattcc attcacttct agcgctcgct gccatccagt cagggggcgt
     6841 gatatttatt aatggttgct gcttgaacaa ttcttctcta gcgtccacac ggattgcttt
     6901 ttcttcactt gatatattgc cttttgtatc gtgtaatatt ttagggtgtc ccatgttgtc
     6961 ctcctttcag acaaaagaaa aaggaacgta agcaattaag ctcacattcc ttcgtttttc
     7021 gatcagaatt tttctttatc taatttatct atataaatga tttttccgtt ctgcatttga
     7081 atggtcactt gaccaaaatg aggtaactca atggtgcgct gtttccctgt tttatcagta
     7141 ataacaattg tatttttcaa taaatctcct actttcatta tatacttttt cacttgtgat
     7201 tacaagtgta atcgttattt gtaattaccc ttatgccttt tcacaacggt tatttgtacc
     7261 aaaatgagta ggcatcgatt gtcagtaagt ttcacactat gcccccttaa ctcaaagggt
     7321 actcttttct cgtctttgtc tttcattggc tgtcttggaa ttatggtgtg catggcataa
     7381 agaacgaaaa ttagtttcat ctaaccgtct attccaatca tcttttagtt caattttgtg
     7441 atctacaaca tcagctttcc ttatcaagcc ttcttctagg caatcctcac atactggatg
     7501 atttaaccga tacaagtatg aagcctttcg ccatgttcta ctttgataga attgaaaata
     7561 tttaccttcg ctttgctttc tttcttcacg cttaattgcc gtttgctttg ctttatgctt
     7621 cccacaatac ttctgcctat aatcaatcaa tgttttacat ccagcatgat tacattgttt
     7681 tttaactgcc atagattacc aaccaaagcg atctcgttcg tcagaatcat cttgttccca
     7741 ttttgatttg tattcaatat aaggcgaaac tatcttcaag cacgttccgt ctttaaaatt
     7801 aactttagtg attttatctt cctcaaaact aataaagtct acatcttctt gagtgtttga
     7861 atactcaatg tgtttctcac catcgaagta tctaatactt tccatatgca taattattct
     7921 acctccaata aattagttat caattcatca tctgttagta gcaaagccaa gaacttatca
     7981 tggatgttta aaaacaaatt atcctcattc gatgtaagct ctcgttgtct cgctcgatca
     8041 tataggttaa gcagacaatt aaattcatcc attagctcac ctgtatcatc atagttgcca
     8101 ctatctacca atgatcccac tactagcgat tgacaccacg ctactgactc ctgcagccaa
     8161 tcaggttctt cttccattat ctttaatact tctggatcaa atgtggaata atcttctgtt
     8221 accaatccca cactaaatgc cagcgtgttc attagaaacc gatccatttc atctgtatat
     8281 tgaaagttcg gtgctttgtt tgttgaaata atattgagca tttactttcc tccttgattc
     8341 gtaacacgtg gtaacaccta cgtaacaggt acccgttacc actcaatccc aacggggaca
     8401 agggttctag cgttttggta acaggtaacg ggtaaaatcc agtaacatta ttccccagca
     8461 tattagctaa ctatttcaaa agttttaagg aaataaggtg ttaccccgtt accaaattgc
     8521 tgtatccctt ggggcagtaa gggtaaacta gtaacgggct aggtgttact gacccgttac
     8581 tacccgttac tttttgtact ttccagctcg atttttattt tcataggcat ttgacttatt
     8641 gtaaaatcct cgtcgtttat ttctttttgg cagttgcttc cgttcccaac caggaatact
     8701 gctaataatc aaggcaatct ttttagtttc tgcatctgat cgaccattca ataaatcctt
     8761 tgcttgttta tcaaaaacag cttccaaaat atcagcggtg agtacgctat caactaaata
     8821 aaactcttgt tgcatagtag ctccattata gaaatgctgt aaatttttct cattacattt
     8881 actgtcagga tagttctgat aataagaacg tctcgcccac gctggagctt cttcccattt
     8941 atacgggatt tccatttcca aatatttagt gattgcttct ttaattggat tctccatctt
     9001 ggcatcctct tgatagtctt ttgcaatttc taagacttct ttatcttcat cgggactaaa
     9061 atagattcgt tgtcctttat caaaaagcac ctttgcttca gctaacactt gaagaaaata
     9121 ttcatccttt gttttaaacg gattcatctt tggcttattc ttacaaggca aaggataaaa
     9181 acggcggttt cctgtatcat ctttcaaata ctgcccatca ttcgacgttc caataaatac
     9241 acattgcctt gcccaagcta tgacatcccg accataagga ggtctaattt tatcgatctt
     9301 ggcactgatg aagtttttta tctgttcaat tttagttttg ttcattgaag ataattctcc
     9361 aagttcaata attacattgc cttgaagctg catataatca tctttctttt cacctagcga
     9421 atctaatgaa tcggtgaaaa aatcatccgt gtaaagtaat gctggaacgg tactcttacc
     9481 aatcccttgc ggtccatcca aaactggaac gatctcaaac tttattcctg gtttatagat
     9541 acgtgcgact gatccgacta tccaacgttt tgttacttct cgtgtgtaaa ggttgtcgtc
     9601 tacccctaaa aggtcaataa acagtgtttc cagtctaggc tgttgatccc atttaacttt
     9661 ttcgatacgt tcttttactg ggttgaaaac attctttcta gccacattta ctactgcttt
     9721 gtgtattttg ttgtcatcaa aaactacatt gtagttttct tcaatatatg ccgttaaggc
     9781 atcctcaaaa ctgtcttccc attctccagc ggtacgattg atccaaggag aatcttttaa
     9841 taggtgaatg tatccgctaa attcgttata agcaattgtt ccagctaaat tgctatcatt
     9901 ggttaaaatc aattctgcat tcacaacact gttactttta atctgatcgt ctttattgca
     9961 tcttaatcgt gacatccagg ttttcaattc aatgatattg tcttcacgct cgctggcttc
    10021 gggaaagtct aaaatactat ctaatttatg cttatgagcc atgagctttc cccctcaaat
    10081 cttttttaat catcgatcga ataatttgat tcacttcttt atcttttagc ggtggttcat
    10141 tgtacgcatt tgctgtataa atcaattctg ctaaagtttg attatctaca ccgtgccata
    10201 gtaaccaacc gcataactta gttaaatgat cgtttctact accttcgcca gcgccttgaa
    10261 caatcatatc taattttttt gctgtccctg tttttgctac gggtcggtat tgagtcttta
    10321 tttcttgcgg tttttttagc agtaggttca ctagccaatc gggagctggt gcaatattat
    10381 ttagagatac gttttcaata ggttcatatg ctctcccaac acttcttgaa ataatcatag
    10441 ttggcgctat tggaactcct ttagtgataa tatcaatccc gcttaaactt ttacctttat
    10501 catcacgccc gaagatatca gcaaatgcac ctgtttcttg ttggataatt acaccatcag
    10561 gaattttaaa gaagaaatgg attcctttac ctggtgtgat ttccgtataa gtagaaggaa
    10621 atggatcata cattgaagca agttttttat aattctcaac gccgtttcgt gtttcagaat
    10681 gttgatctag gtcaactacc accatttgat gatcttcaag cattaaccca atatttctag
    10741 ttgcatcttc cgtccaccat tcattaattt gattagaatc gttagttgca tcattccaac
    10801 cactagagcc ttttattgga attttagttc caggagataa gggataaatt gaccatcccg
    10861 acttttgtaa ttccgttgct gcttgataat tatttgccaa taaacctctc ctcctttttc
    10921 aaaatactgg tataaatcat tcagacacct ccatgagctt caactgctca agttcgtatt
    10981 tcaacccatc ccgaacaaat tcagcacctt ttaattgatt ctgatacttt acccatgtat
    11041 taaatgtttt cacccttgct tcttgcatat cagtaatgtt taaccatgcg gttaaaatct
    11101 gattttttag ctcgttgatt tctcgacaaa ttattttgtg ttctgcatgt tttttgagta
    11161 catacaaaac tgttagtttt tcattgaatg aatttccaaa gtaggttttc ccgatattat
    11221 tttctagttc agcgatccct tgattaattt cctctaattc aaggctatcg agttggttta
    11281 atagttttgt tttgtacatc tacttatgcc ctccagttct taattgatag tcgtactcgt
    11341 cataagcagt gaagagatat aacgctaatc ctgcaaagat agctattttt gcaattaatg
    11401 gtaataagcc gaaaattccg ccgattaaaa tgcttcctat aattaagttt cgtttcataa
    11461 tggttccctc cgatttttta atatggtata atcggaacaa agaatatcgg gttttatccc
    11521 aattaagttg cctttatcct gtccgccaag actgttatgg ataaggcttt ttctttgttc
    11581 tcgatcatgt taatgctcct aagttgtctg caattcagca gaacgattat aaattttctc
    11641 actcactgaa aactgcaacc catatttttc tttaacagtc attaattcta ctgtctcatc
    11701 taaaataggt tcacgatcaa ccaacatttc aggagtcata tctttctttt ttaccatttt
    11761 ggaatgacca taaatagttg atacagcttt attggctatc acattcgctt taatgtagtc
    11821 tactggtttt ggttcagtaa tagcgtggct taatttagtc attgcttctt tttggtgttc
    11881 tttgtctagc atgcggaata cttgaaagcc ttcaagtcct gtggactggc gtagttcttt
    11941 gatgatgtcg aaaatccagt cttgaaatgc ctcggcttct tttttacggc ttttgaatgc
    12001 catgcggtaa atattttttt tcgttgatga tgtttacatc ttgtgttctg cccaatgaat
    12061 cgatgacctt actagtagta accccatctt taggcaaaga gtgaatggct ctagtaacct
    12121 gtttaagacc taacgcttcc gcaacatctt tcgccactgc ccaccattca tcattaatat
    12181 cgacaaatct tataatgtgt ccgttccaaa tttcagtttt cattcaacgt cctccagttc
    12241 aagccctagc aagctcgcta acaactcaac ttgctgatac aaaatttctt gatactcttc
    12301 aatagaaagt cgttctttaa actctgcttg tactaaatcg ttcaagccac gcaatactcc
    12361 gcttaccgtt ggttcttgtg gtagcaattt ttgatagtcc atttgttcgt acaaacattc
    12421 aaacgtttct tctagcgctt tgccttccaa gtttgctaat tctgccgctg tgtcttctgt
    12481 aagaattgtt gataatactt tttcgattgc tttattttgt gtcgattcca ttattatttc
    12541 ctcctattaa tccctttgcc cccacggctt gcgtttattt ttggtattct tccataaagc
    12601 gatcacattc atcttgtgaa attcttacga ctccatcaac tataatcaac tttaaaccgt
    12661 taggaacata tttctgcatc atagtattgt atgaaacgtt catatactta gcagcttgtg
    12721 ccttgttcat atattttgga aactggtact ttttttctaa taatgaaagt aacgttcctt
    12781 gagtttgctc tacttgttga acaactcgat cgtcaataat tttaaaagct ggatcatcaa
    12841 gcgtgagttt gtcaattttt aattccatat ttatcccccc tcgttagcat acatttctat
    12901 acaaagattt taaaaaaatg caagaggctc ttgaatacat ttctatacct cttataaact
    12961 caatatacca tcataccttt tcatttgcaa gaattttaac atactttttt gaacttttat
    13021 gtttcacaca tatttaaatc gtgatatagt agagaaaata tatgaatggt ggtgaaaatg
    13081 tggctagaaa aaaatacccc gaagcagtgg agacaggaca aagaattaga ctggctagaa
    13141 ttgaaaaggg actctcaatg gaacaacttg gaggtaaatt atccccaccg gccagtaaag
    13201 gtgccgttag taattgggaa aatggctata atcttcccaa taacaccagg cttaaacaac
    13261 tttcaaaggt attagatgta tccactacct atctattaaa cggatcttat acattaagag
    13321 atatgcatat gatgcccgaa attaagcgaa atgtgcttga aaagcaattg aaaaaaacta
    13381 ttgaaaaaaa taactctata attgattcta atctttctaa tgctatcaat aacatcgaag
    13441 aagataacct gactgtctca cagaagattt atttaacaaa cgcaataaaa tatcttaaaa
    13501 caggatctcg aattgaattt cctgacggga gtaccccaat tgcttatctc gcagctcttc
    13561 taaatacgct tttacgatat gactcattaa ttaatgattc agaaaaaaat acagatgaga
    13621 aaaaagagta tcaagaattt tatgaaaatg atcttctaaa agagttcaca gctcttgtta
    13681 ctgagctaaa taaacactat tcaaaataat aataaactgc ctcctccaac cgcccccacg
    13741 gcttgctaca ctatggaggg aaaactatta tggcaacatt caagcaatat gaaactaaaa
    13801 aaggtaaatt ttggctctat gaagcctatc ttggaatgaa caaaatgact ggcaaacctg
    13861 ataaggttcg tagacgaggt tacaaaacaa aaaaagaagc ccagcttgcc ttgtcacgct
    13921 tgcaagtgga ctacgataaa aacggtatta aaaaagcgaa caacgaaacg tttaaagaag
    13981 tttatgactt gtggctagaa acatataaaa ccactgtccg agaagccacc ttcatgaaaa
    14041 ctgaaatcaa gtttagaaag tggattcttc caaaatatgg aaatcttcgt gtgaatgaag
    14101 tcacggtaaa aaaagcccaa caaattgtaa atagctgggc tgaaactact gaccaataca
    14161 aggtacttca ttcaacttct aagcgtattt ttagctacgc tatcaatctt gggattattg
    14221 agactaatcc actagacaac gtattaatgc ctaaaagaac caccactaag cataaagaaa
    14281 aggtcaaagt ttacagtaaa gaacaactga tcaccttgtt tgattaccta aacagcaaac
    14341 ctgaaactta tcgaaacgac tttgataaaa cgttactacg ctttctcttt tatagcgggg
    14401 ttaggatcag tgaagccctt gcattgaatt ggtcagacat tgattttcaa gaaaatacgg
    14461 tcaccatcaa taaaacacta agccaatcca aaaatggcta taaaatcagt gatcctaaaa
    14521 ctgattgtag tgctggggtt cttccactag atgaaagaac aatttctata ttaaaaaaat
    14581 ggcagattaa tcagcggaaa tatatgctga cacttggtgt aactgatccg actatgattt
    14641 tttgtggtat ctataaagaa ctaatcacac accacgctat ttatgcaaga ttgattacta
    14701 tttgtgaaaa agctatggtt ccatttctcg gtgtgcatgt agcaagacac actcatgctt
    14761 caatgctctt ggacgctgga gctactatgg tagaagttca aactagatta cggcatacaa
    14821 aaattagtca gacgatcgat tgttacggtc acttggcaaa agaaacgaaa gaaaagactg
    14881 ttgaaaaatt agtacaacac ttaaatattg actagatggc atacaatatg gcatacagta
    14941 aaaaataaaa aaaggagaaa ccctatcata tcaaggtttc tcctagcttt atagtctatt
    15001 cttcttcttc cgccatgaag ttaagctcat taaagataat tgacgatagt tggtatttca
    15061 acgatttttg aatagaacta atcactttat taagtctaat tcaccttaag tggtttttta
    15121 gtcgtattca aaaacgtatt caagttgcgt ggcgtgcttt tgataagaaa tcgtaagaac
    15181 tagttaatca aacgacttat aaataatgta acaccactga ttaatcagct atatccaaaa
    15241 aaacaagcat tcattcagat acatcaatct ctatagaata caaagctttc attatctcaa
    15301 accattacca caacttggca agtcggttaa ttgaagaaca cctcattctc cacttatagc
    15361 agtggagcaa caagttgagc tattgttgca acagaagcta agaattctgt actccattta
    15421 agttttttta gactatctac cgcaaacctc agcatagttt ttttaggctt ttctttaata
    15481 gcttcatctt taatggtttc gatatactcc tgagcctctt ctacctcttc ttcaggtaag
    15541 gaatcattca aattctccaa aagcaaatca agctttgata ataattccga catatcatca
    15601 atagaaacaa tcatattatt attgtggcct tgctgtatat tagtgttgta actatttcca
    15661 aatatattta ctttattata tattgaattg ttgctcatat agtcctccag tcgtcctgct
    15721 gcattacttg catcttgtaa cgtcacaaaa aaatctctcc catgttggta aattgattca
    15781 acgatttcaa attctggatt tccatattcg cgccattcgt attcttcctt aaagtatttt
    15841 ccgtaactat ggttaaattg cacagaattt ttaccaattt tccttagaag ttttatctct
    15901 ctctcagaaa taaaattttc ctcaaaagct gattcaacta tctgaaaatc actaagaaat
    15961 gttcctaaac agaagattga ttcattaata gtttgttgaa aattccatgg tattttgttc
    16021 caaaaaccat tcttaattaa agcctctttt ttttcaacaa tctgtgattt tacattttct
    16081 gtatatatct tccaattatt gacgtcttcc ttaatgtccg aaatcgattg accttcataa
    16141 gaagtccctc caccaaaaag tccttgaacc attttatctc atcctctcta taataattac
    16201 tctaaatcca atacttgcta caaatgatag catttcgtgt ggtaacatga ttgccatcgt
    16261 tcctgtttta tttactctga cttcaatact tggtttgttg atgaaatttt accaattgat
    16321 tccatcgatc gtttcatgga atctgtattt actattttca ttgattgtag agattgattt
    16381 attccctctg ttgcacttct gataccttta gtatcaaagt tttgcatgat tgctcgctga
    16441 gctgataaag ctgataatgt ttgacctatt gactctatcg acctcttcat ggaatctgta
    16501 tttaatattt ttattgattg tagagattga tttattccct ctgttgccct tctgatacct
    16561 tcagtatcaa agttctgcat aatttcttgc tggactgata atgtttgacc tattgactcc
    16621 atcgactttt tcatggaatc tgtatttaat actttcattg ctgatagaga ctctgacaat
    16681 ttagtaacac ctgctaaatc gatattacta aaataatttc caatatatcc ttcaaaatca
    16741 tctgacatat cactaaggtc ctcaccagca atatctccag ccaatatttt ttgaaaagct
    16801 ttctctagtt gatttgtata tctcttcaat ttctttatta atttttggta taaagccatt
    16861 tctatttctt tgttatggct tacaatattc ctaccttctc tgattaaact catgtctgtt
    16921 tgaatttctt ctagcttaac atccatatat ttactaaata cttcactcca caaagaatat
    16981 gaatcagata tagtgtgact gttatcttga aataacttca ataattgatc ctgagatatt
    17041 tctttatacc ttacaatatt ctcttcattt tcttgtgtaa ctaaaatata attttctcca
    17101 aagagatatt cttcaaaatc atataaatct atccattgta aaagattctt tgagtcaaac
    17161 tttcctattg cttgtatttg ttttcttttt attgacgaaa tgatttcatt tggaattgat
    17221 tcctctgccc agttatcaga aaaatatgcc ggagctaata aatagattaa ttttcttaat
    17281 gcccattcgt aactatgcaa atacggtaat agttttttcg aaaaaaacaa cgcatttgta
    17341 tctaaaaggg tagtgtagtt taattttgat ttacttgctt caatattgac cttagctagg
    17401 aaaatttgaa atatatgtac ctcttttatt cctatcttac cagtcttatt atattgaaat
    17461 actacgtgta acgaatcatt atagttcttt atgtcaaagc caaatttata atttttttcc
    17521 tgctcctgaa atcttaagat agaatcacta atatatggtg acaacacttt cagaaaggtt
    17581 tcttcgatat tttccattct tcctttttta ggataatata aatattcaac aacttgctta
    17641 ctaatatgtc atcaacttct ttcctttcaa caattttaat tttcctatta acttgatatt
    17701 tgctacataa gccacaacta gcaaacagaa agagaacttt ttctttacct tgataactgt
    17761 gttaattact ggtttctttt ttctatttct ttatagtatt gttcttctac ttttaatatt
    17821 tttttaactt ctctttttat gtcagcatac atagatatac tgttgtcctt tcggttgaag
    17881 cagtgataat tctcaccatc aaactcataa aaaagcatat cacttgagct tccatctgaa
    17941 ttaattaact gaaatttggt attgagtagt aattcatttt tctcgtcatt caactttatt
    18001 aaaataggtt tgatctcttt taaataattg aaatatattt cggttattac taaattaaat
    18061 ttcgctactg tataaacata agagatacgt ggggaatctc taaattggtt atatatatct
    18121 ttccttaaaa catctaactc cttttttaat tgtccgttaa aatcaaaatg aggcattgct
    18181 agaatttcat ccaaatcaaa gtacactctt tgttgatgaa tatttactgg cagatgacca
    18241 tgctgagaat aatttctcat atgtaataaa aacctatacg aaaaatgctc atcataaggc
    18301 ttactcagaa ttttattctt aaaagattta aaatcttttt ctgctaaaaa gtttttcata
    18361 caaacttcaa tggattcagt caaagttttg gctgaactaa ttaagtttat cgtcaaagca
    18421 tttatctgat aataatactg gtcttccgtt aatttttcac ctgtactttc aattaaatca
    18481 ttagtaaaaa ttgtgaagtg tttgagcaaa ttagttaaat tatatctaaa cacattaaaa
    18541 agtttatcca actctataat atgacgtgtt aattctacat aatttaaaaa tattctttta
    18601 tcagattctg ttattgtttc taataatgaa tcaatctgcc ttattagctc gtcatcttgt
    18661 aaattactga caaaaaacct actatcactt ctcataatta tattctccta atatgatttt
    18721 ttcaaaataa tcaatatgta acatagaagt atactctatt gttacatacc ttatttctta
    18781 ttatatcagt atttctctgt gacacataag ggtgtaacat aattatttta aacgaaatga
    18841 ctacaagtag tcattgaagc aaatctccat aagacatttg taaaatcttt taaaaagaag
    18901 cgtcgaaaga aggaactttt cttcaacgct tcttttattg actcaatttt caatattact
    18961 tgaacgaata atct
//
LOCUS       DBNIBO010000038        18642 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.041, whole genome shotgun sequence.
ACCESSION   DBNIBO010000038 DBNIBO010000000
VERSION     DBNIBO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.041"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(185..1168)
                     /locus_tag="AC1Y77_001167"
     CDS             complement(185..1168)
                     /locus_tag="AC1Y77_001167"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIQ1574757.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            1546..2937
                     /locus_tag="AC1Y77_001168"
     CDS             1546..2937
                     /locus_tag="AC1Y77_001168"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIQ1574758.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVAFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            2953..3993
                     /locus_tag="AC1Y77_001169"
     CDS             2953..3993
                     /locus_tag="AC1Y77_001169"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1574759.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            4012..5097
                     /locus_tag="AC1Y77_001170"
     CDS             4012..5097
                     /locus_tag="AC1Y77_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIQ1574760.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            5130..6092
                     /locus_tag="AC1Y77_001171"
     CDS             5130..6092
                     /locus_tag="AC1Y77_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HIQ1574761.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGINLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            6085..7512
                     /locus_tag="AC1Y77_001172"
     CDS             6085..7512
                     /locus_tag="AC1Y77_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIQ1574762.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            7514..8584
                     /locus_tag="AC1Y77_001173"
     CDS             7514..8584
                     /locus_tag="AC1Y77_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HIQ1574763.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            8571..9992
                     /locus_tag="AC1Y77_001174"
     CDS             8571..9992
                     /locus_tag="AC1Y77_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIQ1574764.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            10005..11012
                     /locus_tag="AC1Y77_001175"
     CDS             10005..11012
                     /locus_tag="AC1Y77_001175"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HIQ1574765.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            11024..12028
                     /gene="neuB"
                     /locus_tag="AC1Y77_001176"
     CDS             11024..12028
                     /gene="neuB"
                     /locus_tag="AC1Y77_001176"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HIQ1574766.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            12025..13173
                     /gene="neuC"
                     /locus_tag="AC1Y77_001177"
     CDS             12025..13173
                     /gene="neuC"
                     /locus_tag="AC1Y77_001177"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIQ1574767.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            13146..13823
                     /locus_tag="AC1Y77_001178"
     CDS             13146..13823
                     /locus_tag="AC1Y77_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIQ1574768.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            13774..14955
                     /locus_tag="AC1Y77_001179"
     CDS             13774..14955
                     /locus_tag="AC1Y77_001179"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIQ1574769.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            14968..15945
                     /locus_tag="AC1Y77_001180"
     CDS             14968..15945
                     /locus_tag="AC1Y77_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1574770.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            15945..16853
                     /locus_tag="AC1Y77_001181"
     CDS             15945..16853
                     /locus_tag="AC1Y77_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HIQ1574771.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            16854..17606
                     /locus_tag="AC1Y77_001182"
     CDS             16854..17606
                     /locus_tag="AC1Y77_001182"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIQ1574772.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            17611..18558
                     /locus_tag="AC1Y77_001183"
     CDS             17611..18558
                     /locus_tag="AC1Y77_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIQ1574773.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6633 a         2469 c         3324 g         6216 t
ORIGIN      
        1 gcttatttaa gaagaagttg cttctgctcc caccgtttat tcgtttttaa agaatgagac
       61 aaaagtgttt cttacttttg tcccacgctc ttattcatgc tcagagcgcc aatagataca
      121 aaatcatagc caaagtttca cgaaccctag ctgtcaaaga atcgatattt tattttaata
      181 taggctattt taaaataaat ggtttaaggt attcctctac aaaagccact ctatcaatta
      241 tcatgggttt aaaataagaa gtgatggaga taaccagatt gttcttagcg tcaatataaa
      301 gaacatttcc actattacca attgctgaat atatttcgtt ctcagaatct aatacccacc
      361 ataaatagcc atatctcata ttttgtaagt cttctaaagc actggttcta ggtttgaaca
      421 tctcgtttat ccacatgggt ggtacgattt gttttccttt atacatccct ttatttaggc
      481 ataatacacc aatttttgcc agatcacttg ccgataaaca caaaccatat cctgccgtcc
      541 cgatatttag tggatcacaa aaccagacat tggttttagg agacttatcc attgtaaact
      601 gcttatgttc ttcagctgtt tcagcataat aattgacatg gtttggtatc cctataggat
      661 caaatagata cttattggca aaatcaacag ttgttagctt acttgtctga tacaacaagc
      721 cagttaatat gtgtaaacag acagtttgat agttgaattc gccggttata ccttttcttc
      781 ctcccaataa atctagtgat gtttttgtcc aatcttcact tgaacagact tttgtccatg
      841 gatcgccttt acatttgtag ggtgctttca ttgttagcaa gtgcttgatt gtaatagcgt
      901 aaatagtttt ctccccgcgt ttcactttat actctggaaa ataatctaat actttatcat
      961 cgatactttt gattaatcct tgctctaaag caatgccaat cagcaaagat acaatacttt
     1021 tcgttgctga catgatatga caagtatcgt cttttttata accattccat tcatctgtat
     1081 aaacttctac cccatcctta tatgcgacaa tttgacatat atttggttgt cgaaaaccaa
     1141 taaaatcatg caactcttgt ttattcataa gatattacct cactttcttc tataagcata
     1201 atgtcattaa aaatcgattt caattttttc gattcttcac gtaatacagg tattcttatg
     1261 gactagacag cgctttggga agatggattc tctgaaaaat ataaaggttc actttatgag
     1321 tggataaaaa aagcagcaaa tacttcgact tctttaattt accaacatta gtgaaaatcg
     1381 attcatctgg agatttgtaa tttagagtcc ttgtcttgat tagaggtgta taaagctttt
     1441 tattagacat ttttaaattc cattacaatt tgcttataaa ctaggttttt aaaggtgact
     1501 atggtaagat aatagagata ttattttgtg attaggagtg tatatatgaa taaaaatgga
     1561 gaatggagtg ctgcacgacg gatattcatt atattaatgg atgttattgt atacaatgca
     1621 gcaatatata gtagtttctt actgaagttt cgtggagaga tcccattacg aaatttagaa
     1681 actttccaac actcagcaat tattatttca gtgattttta ttgcgttgaa cattttgttg
     1741 ggtgcatatg ttttttataa tcgaatgatc agtgacatcg tgtttgtcac agtcatcggt
     1801 caggtattga tgacactagg gatcatggta gtggcttttg cgggccgctg gtttgctttt
     1861 ccacgtcttg ttatcttact ttcatttgtt ctcggtacgc ttttcttaag tatctatcgt
     1921 attctcgtat ataaacttta tttacgcgta agtcgagata aaaaggtcgt cattattggg
     1981 ttagaaaagg atgtcgcacc agcaatccaa aattttggtt caaagaaaaa caacaaacat
     2041 aaagtcgaag cagtcattct tgataactat tatgcaaaca tcaaaaatat gattaaagac
     2101 atcgatattg tctatcttgc ttctcatatc gaagaaactg aaaaaatcaa aatctatcaa
     2161 ttagttacta aaaatgaaaa aaaattgttt ttgaatacaa catttgaaaa cttgacattg
     2221 atcaatccaa atattatgaa tttcgaagat gaaagtatta ttgaagcatc tggatttaga
     2281 atccaacctg aatatgaact attcaaacgt ttgtttgata tcatcgtttc acttgtttta
     2341 ttagttattg cttcaccatt tatgttattc acagcaattt tagtcaaagt gacatcaccg
     2401 ggaccaatca tctataaaca agtgcggatc acaaaaaatc aaaaggaatt ttcaatctat
     2461 aaattccgct cgatgaccgc cacagccgaa gcaaaatctg gaccagtttt agctaagagc
     2521 aatgatgcac gagtaacgcc tgtaggtaaa tttattcgag ctgtccgctt tgatgaatta
     2581 ccacaaatct tcaatgtgct gaaaggcgat atgtcgatcg taggaccacg tcctgaacgt
     2641 cctttcttcg tcgatcagtt caatgcagaa aacccttatt actacttacg gcataatgtt
     2701 cgtgctggta tcactgggta tgcacaagtc tatggaaaat atgtctctga ttacaacagt
     2761 aaattacgtt ttgatttact ttatatcaaa aaatattcat tgatgcttga tttaaagatc
     2821 ttgctgcaaa ccatcaagat cctatttgat aaaatgtcct ctcaaggact tgaagaagac
     2881 gaaaatgtga tctcattggc agaaattgct tttccgaatg aaaaaatctt tcgctagttg
     2941 ggaggcgtaa aaatgaacaa tccgaaattc agcataatcg taccagtata caatgcagaa
     3001 gcaactattg gtcgaacgat tgatattcta aaagaattgg attatgaaga gtacgaagtc
     3061 gttttagtaa atgatggatc aacagatcaa acacaggaaa tcattcaaga actgattgaa
     3121 aatgatgaac gttttgaatt gattactacg accaatcaag ggcccggatt agcaagaaac
     3181 gaaggaataa gacagtcaaa aggagaatat ctcttattct tcgatgcaga tgacacgcca
     3241 gtaaaaacga tcttgtcaga ttatgatcat ttgctagaaa agagtccaaa agcggatctt
     3301 gttgttagtt cgtttgtttt tcgtacaatg gacgagggaa gaatcgtttc cgaaaaagaa
     3361 aacttagtta cagaatgtca ctatctaact cacgaagagt ttataaaaga catgtatcag
     3421 ttaatgaatg aacagttgat gtatgttgta tggaacaaat gctatcgtag cgatattctg
     3481 aaaaataacc aaattctatt taaaacttat aatagttgtg aggatcgaat atttaactta
     3541 cattattatg aatactgtca tcaagtgatc atgaacccaa aagtagaata tatttatgaa
     3601 tttgaaggtg ggaaggggat cactaatcaa taccgaccta acaagttcga aacatttaaa
     3661 gaattctatt cattagcaaa tactgtaaca gacgatatta ataaagacgg tatggcggcg
     3721 cttcttttaa aagggacaac ttcagttatt ttttctattt atagtacctc tgcaagaact
     3781 tcaaaagaga agaaagacga agcaaagcag atattgagtg atccagtgat ccgagaagcc
     3841 aagcaaattg ctaagacgga ttcttcagca aaaaaaataa caaagttgct ttacaatatg
     3901 ccagctccac tatttttatc tgctgtaaaa gttggaagct tcgttgaagt gaaaatgcca
     3961 gggttaatgg ctgctttgaa gcgagtatat tagttaatgg aggaaaatat aatgaaaaga
     4021 attttgcatt ttcagggacg aatgggttta ggtggagcag agtctttcat gatgaattta
     4081 tatcgtaaaa ttgatcgtac aaactatcaa tttgattttt tgatttatga agattatgca
     4141 gatgttcaag actatcattc agagattgaa agattaggag gccgtatttt tgttgtacct
     4201 aatcctaaga aaaatatttt taaatattta atagaagtga ataagttact aaaaaaagaa
     4261 tctttttcta tagttcacaa tcaggtttat tttggcgggg gaattaattt atggttagcc
     4321 aaaaagaatg gaatcagaca gagaattgct catagccatg caactgaaga tggaaaatct
     4381 caaaatattg taatgaatgt attgcgtaaa tttttaacca aattgttgtt acaaaatgca
     4441 acagactacc ttgccgttag tcaagaagct ggtgaaagtc tgtttcaaaa ccatcccttt
     4501 gagatagttc ataatggtat tgatttagag ctttatagta aaaattcaga agctaaggtt
     4561 aataagagaa aagaattgga tatctcaatg agtacttttg ttgtgggaaa cattggacga
     4621 ttagagaaac aaaaaaatca atcttatttg ttagagattt ttttagaaat actaaaaatg
     4681 aattctgatt cttacttgat tttagtaggt gaagggtcac ttagaaataa actgaaagcc
     4741 aaagcagtag aattggatat agagaaaaga gtaaaatttt taggagaaag aaacgatatt
     4801 cccgagttat tatctgtgat ggatgttttt gtaatgcctt ctctttatga aggtctgcca
     4861 atggttggat tagaagcaca agcagcaaaa ttaaaattag tcttagcaga tactatttcc
     4921 aaagatacaa agttaacaaa taatgtggat tttgtatcat tagatgaaac accacaagaa
     4981 tgggcaaaaa aagtaataaa agagccgtat gctaataaaa taacacaaga actaaagagt
     5041 tatgatgttt cgtacactac tgaacaaatg gagagtatat atagtagaga tcattaaaaa
     5101 tgttcaagat attttaggag tgattatttt tgaaaatatg ttatttgata ttggcataca
     5161 atgatattgg caacttaaag agattggtca atcgattgaa cgaaaatgca gtttttatta
     5221 ttcatataga taaaaaaact gatatagaac catttattga agcatttaaa tcatatgata
     5281 atgttgaatt tttatgtaat aaaaaccgtg ttaaaattta ttggggtggt ttttccattg
     5341 tacaagcaga aatcaattta gtaaagagag cattacaaaa tgagaaatat ttaaaatatg
     5401 tattattatc tggtgctgat tatccaatca aagataatga gtatatttat aattatttta
     5461 aaaagaatag ttcagtggag tttattcgtg gaataaatct agatcaaatt aaacataaag
     5521 aattttatta taaacatata gatgtatatc aaaaacatga ttatcctaga attaatagga
     5581 ataacacaac tgcatttaaa atttttagag ctataattaa tcgttgtctg aggatgatta
     5641 aacttccccc aaaaattaga caccataaat ttgatttgta tcatggttct caatggtggg
     5701 ctttgtcgaa agaatgccta acagaattaa tacaaatgta tgaacaaaat caacaagact
     5761 acctgaattt taaaataggt atgtttgccc cagatgaaaa attttttcat acactttttt
     5821 ttaattcctc gttcaaaaat aaaaatgtta ttggaggacc agatttacca cttgaattga
     5881 aaaatattga agaaactact ttacaaactt cgaaattagc aaatattcat attattgatc
     5941 cttccatgaa caaatggttt gatgaaaggg atatagatca aataaaagaa agtgaaaaat
     6001 tatttgtaag aaaagttaaa tcggattact ctgataaact attaaataga attgataaag
     6061 aaattttgaa agttggaaat atagatgaat agaatagtac ctgttttaga gaagagttta
     6121 ttaagtcgag atgcgtttcg agtaataaat ttaatcattt gtctgcttta ttttatgcct
     6181 agtactttta atgtaactaa tatcccgatg aaaatagctt ttgcttgggg tggaattata
     6241 gttttgtatg aggtttttat aaagaaaaat ttttttaaaa tgcgtttttc ttggctactg
     6301 attggagcaa tgattagttt tatcttaact ataatcatta acagagaaaa ttttttaatt
     6361 ccatctattt ataattttgg atatttgctg attactttga tagtaatttt tcctgcagac
     6421 ttttcacaaa gtgaagagga aaaaaagaaa aaaatggtgt attttaataa tgttttcatt
     6481 atcataattt ttttagctgc gctaatatct atctatcaat ttattttttt gatttcttat
     6541 catgtcccaa caggaacgcc aggattactt gcaagacaag gttttattga acatcggtta
     6601 tttggtgtct atacaagtcc aaatgttgga gctatgtttg gatatacttc tattatttta
     6661 agtttaatta gtgtattaat ttcaaaaata acaaaaacga ttcgtgtttt ttatatgatg
     6721 aattttatcg tacaaatatt ttatattaca ctttcttcgt ctagaggtgc ccaggtagta
     6781 atttgttctt ttttagtatt atttcttttg ttgtttgcca attcaaaatt tagaaagagc
     6841 attagagaag tactacctgt aaaaatcaaa tggatggtaa gcatattatt agttctattg
     6901 gtatattttt cttttggtac taattatgct aaggattttt tagcaacaat tcctgtatcg
     6961 attgatagaa aagtagaagt tatagagtca aatgaatcag ataaaactcc acttccagaa
     7021 aaagaagtaa tattacagca ttctagtgat aactccgaaa tttcagctgg tcgttttacg
     7081 atttggaaag ctgcgcttaa agttataaga ccaactttgt tatttggtta tggagagacc
     7141 gatttctatg ggaatagcga tgcaaaattt ttgaaaaaag tagatcttga tactacagat
     7201 gtgaatgaac taaaaagagc acatggaaac atgcataatg gatttcttca agtacttgta
     7261 tcaagtggta taattgcttt tatatgtatc tatgctttct atgtgttaaa tatctttagt
     7321 ttactcaaaa cattttttag aagtaaagtg aattatgcgt ttcttggtat tgttttaatt
     7381 tttatactat ctatttttat agatgaaatg gtggaggctc atgtactttt caataagcgc
     7441 gatgtcattt caattgtttt ctggtattat ttgggaagca tttcttactt gtatttaaaa
     7501 aaggagaatt aatatgaaga cagctttcat tgcttggact cctttacatt tgattaatat
     7561 attaaatgtt tatcaaaatt tttaccgaga atcaaaagga gatttattta tttattctga
     7621 atttgaaaac gctagaagat atagtgagcg tataaaagaa ttagggctat ttaatgatgt
     7681 tgtttttgtg aatcaccgga aaatcggttc ttcctttaaa cgtaaaatta acttgttaac
     7741 taaccataat caatttttct cggatgattt atccgtttat gatgaaattt ttatacaggg
     7801 ggaaaattat ttcacaaaaa tacttttctc caatgtttac aaaaaaaatc cagatgtaaa
     7861 actaaattat atagaagatg gtttgggtac ttatttgaag aagagtatat tttcaacaag
     7921 taaaaacgtt caaagagttt ttaaaatatt aaataaaaat agcatcttca aaaaaaaaat
     7981 tgacgattat tttgtctatt gtccggaatt gttactttat gatgtaaaaa atataaaaaa
     8041 aattcccaaa atagatgaaa acagtaaatt gatgaaaaac atacaatcaa tctttgggat
     8101 agaagaagtt tttgtcggag ataaatgtat cttttttgat caacctttat tatctgatgg
     8161 ttatggcata gatgaaacag atattttcgc taaagtaaat agtatatttt ctggaaaaaa
     8221 agaaacttta attaagcttc atcctagagc aaggacaaat cgttatggtg aaaaaaacat
     8281 attgaattcc agtttaccgt ttgaagtctt gctacttgaa aatgacttct cttcaacact
     8341 tttgattagt cctatatcta caatttcttt ttcaccatct atgatgtttg aaaagacgat
     8401 caactctatt atgttggcta aattaatttt aaatgaatat acagcattat ctgaagataa
     8461 aaaagaaaca ttgacaaata ttgttctttt ctgtgaacat tataaccgtc tatcagaaga
     8521 aaaaatttta ctcccagaaa actgggcaga attaagaaag ttggtagagt atgggattat
     8581 ttaacacact ttccaagtca aaattagtta agtccagttt ctggtatact atagggaatt
     8641 tttttattaa gggaatttca ttcataacta ttcctatttt tgtaaatatt actactcaag
     8701 cagaatatgg tttgattaat aatttcactt catttgtatc aattttttcg attcttgttg
     8761 ggctaagcct aaattcggca attaataatg caagttttga ctataaagat gacataaatg
     8821 aatttatgtc atcggtactt tctctagcta cactttcgtt aattgtgttc ttattaatag
     8881 ggaacatata ttttttgttt tcaaatgctt tttttgatat gaaccaactt gtatttaatt
     8941 tgatgttatt acaaagtttt gctagttttg taattagtta tatccagtct tattttacga
     9001 ttaactttaa ccattttaaa tatttgattt tatcttttat atctactttt ttgaatattg
     9061 caatctctgt agctttgatg cagacattat attttgatga taaatatgtt ggcagagtag
     9121 tcggcggaac actgtcattt gtgttgatag gtttagtaat ttatttaaga ataatgataa
     9181 agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc
     9241 taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt atgattaatt
     9301 cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata ggtattattc
     9361 taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat ggtgagatga
     9421 gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta gtctttactt
     9481 tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg gctccgaaag
     9541 actattattc tgggttatct ttagtggttc cgataacctt aggctatttc tttcagtttt
     9601 tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt attgcgattg
     9661 gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata cctaggttag
     9721 gaataattgc tgctggatac acaaccgtat ttacgtattt tttacttttt atttttcatt
     9781 ataatatcgc taaaaaaatt acgaataaac aattatttga cacaaaaaaa atatgtatca
     9841 taataatttt tgtatcaatt ttttctttga tacttcatat tttaatcaat caaatgttga
     9901 ttcgttacgt tttgacatca ttattgataa ttagtttagt tatatatttt ttaaagatta
     9961 agaatcgaat tatagatgac tataattcat aggaggagat ataagtgttt aaaggaaaaa
    10021 caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt cagcttttaa
    10081 aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa ttttatatgc
    10141 aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc agagactctc
    10201 aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc gcaatgaaac
    10261 acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg atgggaactc
    10321 aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt atcagtacag
    10381 ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt gaaaaattaa
    10441 taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt gtaaggtttg
    10501 gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa ataaaaaaca
    10561 ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca ataccggatg
    10621 ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca tatgtcttaa
    10681 aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata tataatgata
    10741 cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca ggagaaaaat
    10801 tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat gataaaatgt
    10861 atatcattcc accacactat catgctactg acgaactgta caaagattat gaaaaaatta
    10921 aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa tttattagaa
    10981 tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat cagaagtata
    11041 tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt tagaaagtat
    11101 tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta aaacgaataa
    11161 gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta aagaggacag
    11221 ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta ttagaataaa
    11281 agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg aagaaagtgc
    11341 agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag atttaacaaa
    11401 tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat ctacaggtat
    11461 ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc cagttactat
    11521 tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat tggcgattaa
    11581 cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat tgggatatga
    11641 tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt ttaccttgga
    11701 taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat ttgcagattt
    11761 tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc gagcaatgcc
    11821 ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg agaatttaaa
    11881 tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa atggaatagc
    11941 tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa gtgcagatag
    12001 tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg aacacgagct
    12061 gattatggaa tatataaacc tgttgctgag aatttactac aagataattg ggatgtatca
    12121 tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga attgatctta
    12181 gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa caataaggga
    12241 aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt tgaacaaact
    12301 agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc tgcgatagct
    12361 tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc tggtactgta
    12421 gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc tgctacggaa
    12481 aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt tcccgtcgga
    12541 gctcctagaa tagatactat tttaaataat agattaccta gttttagaga tataaaagaa
    12601 aaatatcatc taaaatttga aaagaaaaaa tacgttttgc aagtatttca tccagttgta
    12661 accgaactag aaaatataga aaaacaaatt ttagctttga tcaattcaat ggaacaacaa
    12721 tctcaaccaa ttatttgcat tttacctaat agtgatgctg gtagcgaaat tatcagaaaa
    12781 atgtaccaga aatatttaaa aacggatatt atttatatta cgaatttaga gccggaggaa
    12841 tacctgacta tattaaaaga gtgcaaattt atgatcggaa attcttcttc tgggataatt
    12901 gaagctgcat cattcaaaat accagtaata aatttaggaa ccagacaaaa tggacgagaa
    12961 caatctaaaa atattataaa tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat
    13021 aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac
    13081 ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga tggggaagat
    13141 attaagtgga ttcaaaaaca aatagcttat taaaatatac tagtgatcca tacattatta
    13201 ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa
    13261 tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa
    13321 taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta
    13381 ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat
    13441 atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa
    13501 ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa
    13561 taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc
    13621 caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt
    13681 ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta
    13741 ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag
    13801 aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa
    13861 gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg
    13921 gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta
    13981 agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat
    14041 attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg
    14101 gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta
    14161 gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg
    14221 aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat
    14281 gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca
    14341 ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca
    14401 ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca
    14461 acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt
    14521 ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta
    14581 gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt
    14641 gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc
    14701 aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac
    14761 attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa
    14821 atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc
    14881 ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa
    14941 aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg
    15001 gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt
    15061 gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat
    15121 agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct
    15181 ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa
    15241 atcaatttat taaatgaata ttatgatgaa ataatcgaag tagctaaaaa gaaagataga
    15301 ttactttatc tatcttctac atttttgaag agaaaagaaa ttcaatatat taagtctaaa
    15361 gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg
    15421 catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga
    15481 gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt
    15541 actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt
    15601 attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta
    15661 gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg
    15721 gattctctat tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat
    15781 gaggttatgc atgataataa ttattcacat actattattg aagatgcata tttagaggag
    15841 attaaagaat ttttcacgct tttggaaaag aaaatggaac cccaatatac ttttgaaaaa
    15901 gataagtata ttattaactt aattaacgac attgaggtaa attaatgatg aaagtaattt
    15961 tttttgggtt agggtcaatt ggaaagagac atctaaaaaa tctaataagc tatggaaaaa
    16021 agaattctat taattttgat ataacagctt atagtagcag tggaaaaaaa gagtttgaag
    16081 aaataacgta tattcaaaat agagacgatt tggatacaca ttatgatata gcttttatta
    16141 caaatccgac ttttaagcat ctggaaacac taaatgtaat tgttgaaaaa gttgattaca
    16201 tatttttaga aaaacctgct ttttctgaga gtattgatct tactcaatat aattatgatt
    16261 acaaaaatat ttatgttgct gctccactga ggtataaaaa tatcatggaa gttgttcgta
    16321 atagtattca aagtaaaaaa gtatattctg taagagctat ttgttctact tatcttcctg
    16381 attggagaaa aggtactgat tatagaaata attattctgc ctttaaatct atggggggag
    16441 gagtagaatt agactgtata catgaattgg attatattat aaacctattt ggtttcccta
    16501 aatcagtaaa aaaacagttt ggtaaatatt cgaatcttga aattaacagt aatgatttag
    16561 caacatatat attgagatac gatgataaaa ttatagaaat tcatttagat tattttggaa
    16621 gagttcctac aagaaaattt caattgttta ctttagatga atgtatcaat gttgacttat
    16681 taaataataa aataagtttt gaagaaaaca ttgttaaaga aatgcctgaa tctgctaatg
    16741 atatgtatga gaaggagttg aaatatttct ttgaaaacgt agtaacaggc aaagaaaact
    16801 ggaatgattt atatcatgca aacgaagtat tgaaaatagc tgaggagaaa taaatggaaa
    16861 ttttatttac tatttgtggt agagctgggt ctaaggggtt taaaaataaa aatttgaaat
    16921 atttggcagg aaagccccta gtccatttcg ctttagcaat gatagatatc tatcaacaaa
    16981 aacatcctga acataattgc acgacttgtt tgaatacgga cagtaatgag ttagtagaat
    17041 tgacgactaa gttaaatccg tctgtgaaaa ttgtagaacg taaacctgaa ttagctacag
    17101 atactgtagg gaaaattgaa gtgatacgtg attcattctt acaactagaa agagaagata
    17161 atatatttga tttaattata gatttagatt tgacttcacc tttacgtgaa gtttctaatc
    17221 ttgaagaatt aatagatgaa catctaaaaa atttagataa agacgtctac ttttcagttg
    17281 tttctgcaag acgtaatccc tattttaata tggtaaaaag aattgaaaat gaagttacta
    17341 tagttaataa atcaagatac acatctaggc aacagacacc tgaagttttt gaaatgaatg
    17401 cctcaatgta ttcttttaaa cctgaatttc taaaaaaaag taattatatt tttgatgggc
    17461 aatgtgggat aataaaaatg agagattatc taattttaga tattgatagt gaagaagatt
    17521 ttaaatggtt agaatttttg tttcctaaat ttttagaaga ttcttcggag cttcaagata
    17581 tctataatta tttatatcgg aggtaaaagg atgagctgtt atcttatcac cggtggggct
    17641 ggcttcattg gttctacctt agccaatcat ttaggagaag aacatgtggt aatcgtcgtc
    17701 gatgacttat caatgggaaa agtagaaaat cttgatatga cgaaaaatat cacttttatt
    17761 gagggagatg tcgctgagaa atccttgatg gaagagatca tgaaagctaa taaatttgat
    17821 tatatctttc atttagcagc agttgctagt gtggcagatt cagttgcccg accagtagaa
    17881 acacatcgtg tgaattttga aagtgtatta atgattttag aattgatccg caagtatcaa
    17941 cctgatttaa aacggatcgt gtttagttct tcagcggctg tttatgggga tgaaccgact
    18001 ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc cttatgcggt tgataaattc
    18061 gctgcagaac aatatgtgct agattattgt catttatatg atgtacccgg tagcgcagtt
    18121 cgttttttca atgtctatgg tccaaatcaa aatccgaatt ctccatacag tggtgtgatt
    18181 tctattctcg tagatcgcta taagaaacaa atcgtaggag aagcaacttc atttacatta
    18241 tttggtgatg gaagccagtc tagagacttt gtttatatag atgatgtaat ccaagcatta
    18301 cttttagttg cgaatgagga agctgcactg gggaaacaat tcaatgttgg aacgggtaaa
    18361 gcaaccacac ttttggaatt gattcatgca atcaatgaaa ttctcggtgt agaattgaca
    18421 cttgaataca aagaagagcg ttcgggtgat atccatgact ctctagcaga tattactaag
    18481 atccagtcaa ttggctatca accgaaatat ggtattttaa gtggaatgac taattatttg
    18541 aaaactgaaa tcaattaaaa catactaaga taagattaga gggtaaattc aggctctttg
    18601 tcaataagga ctgatgagtt ttgtaaaatc aaatctggga ta
//
LOCUS       DBNIBO010000039        18406 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.042, whole genome shotgun sequence.
ACCESSION   DBNIBO010000039 DBNIBO010000000
VERSION     DBNIBO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.042"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            127..741
                     /gene="gmk"
                     /locus_tag="AC1Y77_001184"
     CDS             127..741
                     /gene="gmk"
                     /locus_tag="AC1Y77_001184"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIQ1574774.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            746..1051
                     /gene="rpoZ"
                     /locus_tag="AC1Y77_001185"
     CDS             746..1051
                     /gene="rpoZ"
                     /locus_tag="AC1Y77_001185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIQ1574775.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1304..3712
                     /gene="priA"
                     /locus_tag="AC1Y77_001186"
     CDS             1304..3712
                     /gene="priA"
                     /locus_tag="AC1Y77_001186"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIQ1574776.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3727..4218
                     /gene="def"
                     /locus_tag="AC1Y77_001187"
     CDS             3727..4218
                     /gene="def"
                     /locus_tag="AC1Y77_001187"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIQ1574777.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4211..5149
                     /gene="fmt"
                     /locus_tag="AC1Y77_001188"
     CDS             4211..5149
                     /gene="fmt"
                     /locus_tag="AC1Y77_001188"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIQ1574778.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5149..6507
                     /gene="rsmB"
                     /locus_tag="AC1Y77_001189"
     CDS             5149..6507
                     /gene="rsmB"
                     /locus_tag="AC1Y77_001189"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIQ1574779.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6520..7260
                     /locus_tag="AC1Y77_001190"
     CDS             6520..7260
                     /locus_tag="AC1Y77_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIQ1574780.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7257..9326
                     /gene="pknB"
                     /locus_tag="AC1Y77_001191"
     CDS             7257..9326
                     /gene="pknB"
                     /locus_tag="AC1Y77_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIQ1574781.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9491..10390
                     /gene="rsgA"
                     /locus_tag="AC1Y77_001192"
     CDS             9491..10390
                     /gene="rsgA"
                     /locus_tag="AC1Y77_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIQ1574782.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10403..11053
                     /gene="rpe"
                     /locus_tag="AC1Y77_001193"
     CDS             10403..11053
                     /gene="rpe"
                     /locus_tag="AC1Y77_001193"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIQ1574783.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11054..11692
                     /locus_tag="AC1Y77_001194"
     CDS             11054..11692
                     /locus_tag="AC1Y77_001194"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIQ1574784.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            11847..12701
                     /gene="mreC"
                     /locus_tag="AC1Y77_001195"
     CDS             11847..12701
                     /gene="mreC"
                     /locus_tag="AC1Y77_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIQ1574785.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12705..13214
                     /gene="mreD"
                     /locus_tag="AC1Y77_001196"
     CDS             12705..13214
                     /gene="mreD"
                     /locus_tag="AC1Y77_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIQ1574786.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13469..15043
                     /locus_tag="AC1Y77_001197"
     CDS             13469..15043
                     /locus_tag="AC1Y77_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcsB-like coiled-coil domain-containing protein"
                     /protein_id="HIQ1574787.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(15111..16910)
                     /locus_tag="AC1Y77_001198"
     CDS             complement(15111..16910)
                     /locus_tag="AC1Y77_001198"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIQ1574788.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            17033..17539
                     /locus_tag="AC1Y77_001199"
     CDS             17033..17539
                     /locus_tag="AC1Y77_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1574789.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            17618..18340
                     /locus_tag="AC1Y77_001200"
     CDS             17618..18340
                     /locus_tag="AC1Y77_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1574790.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT         6053 a         3424 c         3987 g         4942 t
ORIGIN      
        1 gacctcttgg tacagggtag cttttttacc aatttctagc tataattaaa gataaccttg
       61 aaagttgcag aaaaaaagtg caaaataaac gataagtaca aaaagactag ttgaaaggac
      121 gtcgtcatgt cagagcgtgg attattaatc gtgttgtctg gtccatccgg tgtaggaaaa
      181 ggcacagtac gaaaagcaat ttttgagagt gaggataatg attttcagta ttcgatctca
      241 atgactaccc gtaaaatgcg tgaaggggag gtcgaaggcg ttgattattt cttccgaagc
      301 aaagaagagt ttgaatcaat gatcgaagct ggagagatgc tggaatacgc agaatatgtc
      361 gggaattatt atggaactcc tttgtcatat atcaacaaga cattggatga aggaaaagat
      421 gttttcttgg agattgaagt ccaaggagca aaacaagtca aagaaaaagt gcccgatggt
      481 gtattcatct ttttgactcc gccagattta gcggaattaa aatcacggat cgtaggccga
      541 ggaacagatg cagatgaagt aatcgaagaa cgaatgaaag ttgcaaaaga agaaatcgaa
      601 atgatggctc tttacgacta tgctgttgta aatgatgaag tgccgctagc agttgaacga
      661 atcaaaaaca ttattgccag tgaacatttt cgagtagatc gcgtaatcgg taaatatatt
      721 aaaatgttaa aggagatgta gtcttatgat gttaaaacct tcaattgact cactattaga
      781 tcgtgtaaat tcaaaatatt ctctagtgat tttagcaagt aaacgcgcgc atgaattgga
      841 tgcaagtgca cagcctactc tagattcttt tgattcagta aaaagcgtcg gtcaagcttt
      901 agaagaaatc gatgcaggaa atgtagtcaa tgatccacat cctgaattaa aacgtgaacg
      961 tttgaagatg gaagaagagg aacgtcaagc acaaaaagag cgtgaacaac acgaattgga
     1021 atcacgtatc cgtgaagagc aaaaaatgta ataaagaaaa atagagcgaa catctaaaaa
     1081 gaaaagcaaa cccagctgtt tccttttcaa acattcgcca cttattgtct ggtagaaaag
     1141 gctgtgtctg aactttttga cacagttctt ttttttctta tctgctgtta attttttgtt
     1201 aggatttggc agaagataag acaaatccat cgatctctag taaaataggc aaggaacttt
     1261 ttgtcgcttg ataagaaaag acaaaaaaag gaggaaacga accatgacat taacagctga
     1321 agtcattgtg gacgtaccta ctatgcagac cgatcagcca ttcacttatt tggttccctc
     1381 tgaaatggaa acagctttgc aagtcgggat gcgagtagaa gtcccttttg gtaatggcaa
     1441 tcgtcatgtc caaggatttg ttatggctat ccaaaaatca gaagaatcag ctaatccatc
     1501 tttgaaggca gttattcgtt tgctagacct tgctccggtg gtcaatgaag aattactttc
     1561 attagctgat tatatgaaaa aaattactta cgcattcaaa atcacttgtc tacaaacgat
     1621 gcttccgagt gtgatgaaag cagaatatga taaattgatc tatcctttgg cagacacacc
     1681 agaagttcag gtattgtttg atcagcgaga agtgatttcc tggaaggaag cagaagaaga
     1741 aggcagcctt tctcaattga ttcgctggcg ccaagagcaa ttagtagata tcaaatatga
     1801 agtccataca cgaaataaag taaaaacgat tcgtttggtc cgttctttgt taactgaaaa
     1861 gcaaatcgaa gaagaatggg caaaattacg gcaaaatgcc aagaaacaaa aagaacttct
     1921 tttgtgttta tctgaactga gccaagaaga accaattgct tatttcaaaa ataaaggaat
     1981 cagtactgct gtcttgaatc aaggaaaaga aaagggctgg ctggagttta tagaatcaga
     2041 acgttatcgc gacccttata aagaccgagt atttgaccaa acgacggcat tggaactgaa
     2101 tgcagaacaa aaaaatgctg tcgaacaaat catcacagca ggtcagcagc aaaaagatca
     2161 ggtctttttg ctggagggaa tcactggcag cgggaaaaca gaagtatatc ttcaggcaat
     2221 cgctgatgtt ttgtcagaaa ataaaacggc tattatgtta gtgcctgaaa tcgcattgac
     2281 tccgcaaatg gtcgagcgat tcaaaggacg cttcggagaa tcagttgcgg tcttacatag
     2341 cggactttcc caaggtgaaa aatatgatga atggcgcaag attgagcggg aagaagcgca
     2401 agtagtagta ggcgcacgat ctgctatttt tgccccgctg aaaaatatcg ggctgatcat
     2461 tatcgatgaa gaacatgaat caagttacaa acaagatgag acaccgcgtt atcatgcgag
     2521 agatctagca atttggcgca gtaaatatca tcattgccca atagttctag gaagcgcgac
     2581 gccctcttta gaatcaagag cacgagctca aaaaggcgtt tatcagttat tgcagttaaa
     2641 ccaccgagcc aaagcagctg cacagcttcc agctattgag atcgtagata tgcgtgaaga
     2701 gtttcagaat catcggacct ctactttttc tgctaatttg caggaaaaaa tacaaaatcg
     2761 attagacaaa aaagaacaaa ctgtcctact gctcaatcgt cgaggatatt cgtcttttgt
     2821 gatgtgtagg gactgtggat ttgttcttcc atgtcctaat tgcgatatct ctcttacact
     2881 gcatatggat acacgttcaa tgcgctgcca ttattgcgga catgaagaac ctattcccaa
     2941 tcgctgtcct aattgtggcg gcaataaaat ccgctactat ggaacaggta ctcaaaaagt
     3001 agaagaggaa ctccgagaac tttatccgca agctaggatt ttgcgtatgg atgtagatac
     3061 cactcgaaga aaaggggcac acgaacagat tttacaaaaa ttcggtgcaa aagaagcaga
     3121 tatattatta gggacacaaa tgattgctaa aggtttggac tttccagaag tgacattagt
     3181 tggggtgttg aatgctgata cctccctgaa tttaccggat tttcgttcta gtgagcatac
     3241 attccaattg cttacccaag tttcaggacg agcagggcga gcggaaaaag caggcgaagt
     3301 cgtgatccaa acattcaatc ctcaacatta cgccatagaa ttagctaaaa aacaaaatta
     3361 tgagcagttt tatcagcagg aaatgcatgt acggcatcgt ggaggctatc ctccttatta
     3421 ctttactgtg aaaataaccg ccagccatcc tgaagaacaa gtagcggcaa agaaaatctt
     3481 tcagattgcc aatcagctaa aagaagtgtt aagccctcaa agcttgctat tagggccaac
     3541 gcccagcatg attttgagag tgaaaaaccg atattattat caactaatca tcaaatataa
     3601 acatgaaccg aatcttcctc atgttttgga tgaaattttg aatggaagcc agaaagaaca
     3661 gcgacaaggt ctttttgtgg caattgacaa cgaaccatta aactttattt aagaaagggc
     3721 caaattatgc gctatcctat attgatccac cctaatgata aattaaaaag aactgctcag
     3781 ccaatcgatg taatcacgga cgagacaatc gctttattag acaatttata tgagacaatg
     3841 attgcgaatg acggaatagg gatcgctgct cctcaagtag gacaaaacaa gagaattgct
     3901 gtgatcgaag tagatgaagg cgagaaattt gaactgatta acccagaaat catcgaagca
     3961 aaaggtgaaa gccttgacgt agaaggttgt ctcagtattc cgcatgtcta tggaacggtc
     4021 aagcgagcag atgaagtgac cgtgcgttat tatgatcgag acggagaaga gatagaagtg
     4081 acggcttttg gctatttagc ccgtgccttc cagcatgaaa tcgatcactt agacggtatc
     4141 ttgttcattg agaaaatgat acagcagatc ccggaagaag aacttgaaga atatatggag
     4201 gaacatcttg atgactaagc tagtatttat gggcacacct gctttttctg tgccgattct
     4261 tgaagggctg atagaatcag gttacgagat ccaggcagtg gtaacacagc ccgaccgtcc
     4321 agttgggcgg aaacgagtaa ttacgccgac accggtaaaa gaagcggctc tgaagcacgg
     4381 tatccgtgtg ctgcagccag agaaaatctc cggttcgcca gaaatggaag aaatcattga
     4441 attggtgcct gatgtgatcg tcactgcggc atttggccaa ttcttgcctg aaaagttatt
     4501 gcaagtacca aaattagggg caatcaatgt ccatgcttct ttacttccta aatatcgggg
     4561 aggtgcgcct gttcattatg ccatcatgaa tggggagaaa gaaactggtg taacgatcat
     4621 ggaaatgatc aaaaaaatgg atgcaggcgg aatctatgcg caagaaagca taccaatcac
     4681 taaacaagat gatgtaggga cgatgtttga aaagcttagc cttttaggaa gaaagctatt
     4741 gctagaaaca ttgcccaata ttttggatgg acaaaaacca gtacctcaag acgagtcaga
     4801 agcaactttt tcgccaaaca tcacacggga acaagaagca atcgattggc ataaaactgc
     4861 agaagaaatt gacaaccaag tacgcggaat gcgtccttgg ccaattgcat tcacgaccta
     4921 tgagggcata cgctggaaat tattatcggt tgaagtattg gcagaaacta cagacaaagc
     4981 acctggtacg atcatccgga aagacaaaaa aaatctatgg attgcttgcg gtaaacagac
     5041 cgtcttagcc atcaatgaat tacagccagc tggtaaagga aaacaggccg tacatgattt
     5101 ccttaacgga agcggtcagc aagttcagat tggtcagcag gtgaaataat gaaaaaaatc
     5161 cctaaaaaaa tgaaaaaatc agtacgatat atggcattac tgacattaga gcgagtgaac
     5221 aagggtggcg cctactcgaa tcttttgttg aatgagatga tcaaacaaag cgagctaaat
     5281 caaaaagatg tgggtttatt tacagaattg atctatggta cgattagcca tcagcgacta
     5341 ttggaatttt acgttgcacc attgatcgct aaagcaaaaa aagtggatga ctgggtcaag
     5401 acgcttcttt atctttctgt ataccaatta gaatttttag ataaagtccc tgcacatgct
     5461 attttaaacg aggcggtcga aatcgcaaaa gtaaaaggaa atcctgggac agggaaattt
     5521 gtaaatggtg tcttgaggaa ttatcagcgt caaggagcac cagatctgca aggcattgct
     5581 gatcctatcg aaagactttc tgtcaaaatc agcctgcctc tatggatgac gaaacgactg
     5641 gtggatcaaa tcggatacga agaaactgaa aagctcggtt tatcgctata tcagcctagc
     5701 catgctagtg cccgagtaga tacacgccga ctttccagag atcaggccat cactgttttg
     5761 caagaagaag agatcacagc tgaaaaaagc caagtcagtc catacggtat agtagcgaaa
     5821 aaagggcatt tagcttctag ctcattgttc cacgatggcg tcatgacgat ccaagatgaa
     5881 agttcgatgc ttgtagcgcc gtctatgcaa atcgagcctg aacatcatgt cttagatgcc
     5941 tgtgcagcac ccggaggtaa gacgacgcat attgcttctt ttttagatcc gtcatgcggt
     6001 ggagaagtgg tcgctttaga tgtacacgaa cacaaagtca aactcattga agataatgca
     6061 gcacgtctag gtgtctcttc tgctgtctac gctcaaaaga tggatgctag agaagtcagt
     6121 aaacagttcg aagcagagca gtttgatcgg attttagtag acgcgccttg ttcaggtctt
     6181 gggctgcttc ggcgaaaacc agatattcgt tataaaaaac agccggatga attagcgaat
     6241 ttaccaaaaa tccaattgga aattttagaa agctgcgcac caactttaaa gtcttccggt
     6301 attctgactt atagcacttg tacgatctta aaagaagaaa accaagacgt tgtttctgct
     6361 ttcttggaaa gacaccctga ttttgagagg attgatgttt tgaccaatcc tatgctacaa
     6421 tcatcaatcg aagagaaaat gttgactttg tatccgcatc agttttacac agatggattt
     6481 ttcatttgtt gtttgcgcaa aaagtaatga ggtgaaaaaa tgcagattga atatcaatca
     6541 gatgtaggaa gacgacgtaa cacaaatcaa gactatgcca gtgtatttac cagccgttca
     6601 ggtctcaagc ttgctgtatt agcagacggg atgggtggtc atcgtgccgg agacattgcc
     6661 agtcagatgg cggtaacgaa tctaggcgaa gcttgggaaa aacaagaagc tgttgacgat
     6721 gaaaaaatcg cccaatggtt cattcaatcg attcaagaag aaaatatgct gatttatcag
     6781 cgtggacaag aacagccgga atataacggt atgggaacaa caatcgtagc ggccgctctg
     6841 tcagatgact ggttcacgat tgccaatgtt ggggatagca gagcttattt gattcgtgaa
     6901 gataaaatca tgcaattgac ggaggatcat tctttagtca atgaactagt caaatcagga
     6961 gaaatcagcg aagaaatggc agtgaaccat ccaaggaaaa atattttaac acgttctgtc
     7021 ggcatgcctg gaacagtcga agtagatgtt tctactcatc tttggcaact aaaagatcga
     7081 ctgctgttat gttcagatgg cttgacgaac atgctgtctg aagaagaaat aggaacgatc
     7141 atcaatcaag aaggcccatt gtctcataaa gtaactgagc tgattgatcg tgccaatgaa
     7201 gcaggcggag cggataatat caccgttctt cttatcgaat ataaggagga cagtgtatga
     7261 tcgagttggg caaaaaatta aatggccgtt atcaaatcac tggaaatatc ggtagcggtg
     7321 gtatggcaaa tgtatttttg gcgcacgatt taatcttgga tcgtgatgta gcagttaaag
     7381 tcttacgatt tgactttcaa aatgaccaga ctgcgattcg acgatttcag agagaagctt
     7441 tagcagcaac agaactcgtt catccaaaca ttgttagtgt ttacgatgtt ggtgaagaag
     7501 acaacatgca gtatctggtg atggaatacg tcaaaggaat ggacttgaaa cgatatatcc
     7561 aaacacatta tcctgttcct tatgaaacag cagtaaatat tatgcagcag atcctttctg
     7621 ctatttcttt agctcataac catcaaatca tccaccgaga tttgaaaccc caaaacgtac
     7681 tgatcgataa tgaaggtgtt gtaaaaatca cggatttcgg gattgctatc gctttatctg
     7741 aaacatcgat cacgcagacg aatacgatgc tgggatctgt tcattatctg tcccctgagc
     7801 aggccagagg aagtatggca acgaagcagt cagatattta tgctttaggg atcatccttt
     7861 atgagatgtt gacgggtagc gtaccgtttg atggtgaatc tgctgtaaca attgctttaa
     7921 aacattttca agatgatctt ccatctataa aagcacttga tccaaatgtg ccacaagcac
     7981 tggaaaacgt gattttacgg gcaacggcta aagaacctgc agaccgttat aagtcagccg
     8041 aggaaatgtc tgatgatctt tctacagtat tatcaccagc tcgtgcaaac gaagaaaaat
     8101 ggcagcctca tgtgatggat aatgaaacaa aagttatcac accccttacg gaagatgcac
     8161 caatgcctga ttcattcaaa tctatgcctc tgccaaaaga caagccagaa aacgaaacct
     8221 tgccagagga ggaagaagtt ctgcctgatc ctaaaaagaa aggacagaaa aaatggtggc
     8281 tgatcctatt agctattctt gctgttttag gaatcggaac agctgtattc tttgcttctg
     8341 gcgggcgagg agaagttgtt gttcctgatg tttctgatct ttctgaagct tctgctagag
     8401 aaacactcgc aaaagcgaaa ttagaagtag caacaaaaac agaagaaatt gcagatgaca
     8461 aaatcgaaga aggtaatgta gtgaagacag acccggcagc gggaacgaca gtcaagcaaa
     8521 aacgcgaagt cactctgtat attagtagcg gcacgaaaaa aatcaaatta gatgattaca
     8581 gtggtaaaag ttatgaagat gccgaagcaa gtttgaaaga attagggttt tctgctgact
     8641 tgattcaaaa agaagaggaa ttcagttcag aggtagaaag cggaatgatc atttcgcaat
     8701 ctccatcaga agatacagaa gtcgatccta aaacagacac gattactttc acagtcagca
     8761 aaggaccaga aacagtgact ttgagtagtc atgtaggaga aaactacgaa actgttcgta
     8821 attggcttgt tacacaagga ttcaatatca ataatattag tgctacttat gactatagcg
     8881 acactgtacc atcaggttcg atcatcagtc aggatccagc ctcgggtgaa gttgtagcag
     8941 aagaaactta catcaatttt gtcgtaagcc aaggaacaga gcctaaattg aatgatatct
     9001 ccggctatac aaaaagcgaa gcacaaagtt atttagctag tgtcggtgca gaatatatcg
     9061 ggcatgagac ttccgaatat tcaaacacgg tggataaaga taaagtgatt cgtacagtac
     9121 caggggcagg aacgacaatt accaaaggaa tggttgtgaa cgtaatttac tctaaaggac
     9181 cagatccagc gtcttctagt tctgaaaaag actcttcaga cagttctagc agctcaagta
     9241 gctccagtag cacaagcaac tcttcaaaag aagaatcttc cagctccaca agttcatcag
     9301 ctagcacctc agaaagttca aaataagtgc tgaataaaat atatcagaag ggaatgcgac
     9361 attgatttgt tgcattctct tttttgtgtt tctatctctt ttgatcaatt ttcaagccat
     9421 atactttgag aagtttgcat ggaataacac ctactttcct cttcgtatgg taaaataaac
     9481 aaaaacggag gtggcactta tcaaaggaca aatcagaaaa gcaatcagcg gtttttacta
     9541 tgtatacgca aacggagaga cctatcaaac aagaggaaga ggaaatttcc ggaatcggaa
     9601 aatcacgccg cttgtcggag atcaggtgat ctttgagagt gaaaatcaaa cagacggcta
     9661 tcttctggaa atcttgccga gaaaaaatca attagttcgc ccaccagtca caaatgttga
     9721 tcttggaatc attgtcacaa gtatcgtaga gcctgcattt tcttataatc tacttgaccg
     9781 ctttctagtt accttggaat acgagtcgat cgaaccgatt atttttttga caaagaccga
     9841 cttgtcagac gacccagaaa aaatcgaaga gatcaagcaa acctatacta aaataggcta
     9901 tccggtgatt gtacccaatc atacgggtga tacgacagaa ctgatccgtt acttcccaga
     9961 acggctatcg gtctttatgg gtcagtctgg agcaggaaaa tcgacgctac tgaatcaaat
    10021 atcaccggaa ctgaatttag caacaggaga gatttctgat gcacttggaa gaggacggca
    10081 tacaacacgt catgtggaac tgattcctat ctatggcggg ttagtagcag atacaccagg
    10141 atttagctcg attgactttt tgacaattga agcaggagaa ctgccgagac aatttcctga
    10201 atttgtagaa gcttctgtcc attgcaaatt tagagagtgc atgcatgcta aagaacctgg
    10261 ctgcgaagta aaacatagag tagaaacagg tggtatcgca cagacacgtt acgacaatta
    10321 tttgcaattc ttacaagaaa tcgaaaaaag aaaaccaatg tatgcaaaaa aagaaggtaa
    10381 acgaaaatag gaggaaacaa gaatgaagat agcaccatcc attttgagtg cagattttgc
    10441 caatttacaa agagacattg agttagtgga aaaaggaggc gcagattaca tccacgtaga
    10501 tgtaatggat ggccaatttg tcccaaatat cacattaggg ccaaatattg ttcaggcaat
    10561 ccgtcctgtt actaaattac cgttagacgt ccatctgatg atcgttgatc cagaaaacta
    10621 cattccagca tttgcaaaag ctggagcaga tatcattact gtccacgtcg aagcaactcc
    10681 tcatattcat cgggcacttc aaatgacaaa aagcttagga gttaaagctg gagtagttat
    10741 caatccagga acaccagtct ccatgatcaa acatgttttg cctttggctg atcaagtact
    10801 agtcatgact gtcaatcctg ggttcggcgg acaaagcttt attgaagaaa cagtagaaaa
    10861 aattacggaa ctcgctgatc tacgtagaga gaacaattgg acttatgcaa tcgaagtaga
    10921 tggcggaatc gttccagaaa cagcaaaaat ctgtaaagat gcaggggcgg atgtcttcgt
    10981 tgcaggatca tatatctatg gagccgaaaa tccagtcgga caaatcgaac gattaaaaga
    11041 ggcattagac taaatgaaaa ttttattagc tgctggaggt aatccgcaaa actggccgca
    11101 gtctctgctg tccaaatacg atgcctatgt cgggatagac cgtggtgcgc tgtacctgct
    11161 tcaagcaggc tacccagtag atctggcagt gggcgatttt gattcattgt cgaacgaaga
    11221 gcagaaggaa gtatttgcaa aagccaaaga gtatacccgt tcccctgcag aaaaagacga
    11281 tacagataca cagttaggta tcttaaaaac atttgaacgt tacccgcaag cggaagtcac
    11341 tttgattgga gctacgggcg gaagattgga tcatctttta gccaatctct ggttaggtat
    11401 ggaacctcgc ttcaaaccct ttgcccatca gcttattatc agagattgtc aaaattattt
    11461 gtcttattat ttgcctggag agcatatagt aagacagata ccagatatga agtatttagc
    11521 ttattgctgc ttgactcccg tctcgaatct tacattaaaa ggcagcaagt atgtattgga
    11581 aaataaagaa gtagaatatc cgacatctta tgcaagtaac gaatttcttt cagataccgc
    11641 agaattttct ttttcagatg gtatgattgc agttattcaa agcaaagatt gatccataaa
    11701 caaataaaaa aatttgtctt tcaggctaaa aatgacagaa tctattattt gctaaaaaaa
    11761 agcttaagag gagcaactta ccttttgttt cacggcattt agtgataaaa ttgaacacaa
    11821 gagtctaatt aattcgaggt gtctttgtga agaagttcaa tcctaataaa aatattatta
    11881 ttaccttggt tattgttatc atcgtggtga cgatactaag tgtgaccgct gcgcagcgag
    11941 cgaatgaagg aaagcccaat ttcttccaat ccatcgttaa tgacagtgtt gcaatggttg
    12001 ataaaacaat ctctgcccca gtacggtggg tacgaaatgg agtagattct gtccataatc
    12061 ttttttcaac ttatgccgaa aatgagcggc taaaagaaaa aatcgatagc tatgatgacg
    12121 tttcacagca aaacaagaat tacgagagag aaatcgaagc gttgaaaaaa gaacttgagt
    12181 tgaatcagac gctgactagt tacgaaaaag taacggcaaa tgtcattact cgttcgcctg
    12241 acagttggca ggacatgctg atcgtagaca agggttctaa agacggtatc gaagcaaata
    12301 tggcagtcat gtcgcaaaaa ggattgattg gccgagtgat cgaggtcaat acggcttcgt
    12361 ctaaaatcga attactgtca tcctctaatg aaagctccaa tcattttcca gtacgggtat
    12421 cttcggctaa tggcgaagcg tttggtttgc ttaaaaacta tgatgaaaag ctccatgcct
    12481 tagtggtgac ccaattaact ggtgatacgg atatcaaaga aggggatgtt gtccagacat
    12541 ccggtcttgg agggaattct ccagctaact tgccgatcgg tacggttatt aaaacgaaac
    12601 cagatagtta tgggctggat cgggaagttt atgtgaaacc ttatgcagaa atgtatgacg
    12661 tgtcagttgt gacgattgtc caacggctag cggaggatta gtccatgttg aagaaagaaa
    12721 caatgaagta ctatctgcca atcgttttgt tctttttgat gttgatagat ggtcatttaa
    12781 caagaatgct aggggagtgg tcgaaaggca cctatatgtc aaatgcccac tttctgatat
    12841 tggcattatt atgttgcagt atggcgtttg aaaaacgtta tttactgatt accacgattg
    12901 ttctcggggc tatctatgac gcttactata ttggcgttat cggtatctat gcagtagctc
    12961 tccctttaat tgtatggttg atgtatgtaa tgaaagacgt tatccatgtc aacatcttta
    13021 ctgaattttt cagtatgatc atctttgtca cgggttatga attgtttacg atggtggtcc
    13081 agttgatttt taaattagca gtagtaaata acacgtattt tattacaagg tttttaggac
    13141 ctacactgct gttgaacatg attatatttg tattattcat ttttcccttt aagaaattat
    13201 tcagtgatga ataatttctt tttttatttt taggtcgaaa gcgaaaaaga atcgttgtta
    13261 tcttaagaaa tgctagactt aacgcggctt tttcatgatc attttctgaa acgaacagtt
    13321 ttgtcatttt taaaagttgt tttgaaacat ttttgtaaca taactattat atcgttgaca
    13381 cactgctatg gtacaatttt tatatcgtta agaaagactt aaatatattt agatatttat
    13441 taaattaagc cagtcggagg aatgaagagt gaaaaagagt ttaatatcag cagtaatggt
    13501 atgttcaatg accttgactg ccgtagcatc accgatcgct gccgcggcgg acgattttga
    13561 ttctcagata caacagcaag atcaaaagat tgcagacttg aaaaatcaac aagcagatgc
    13621 acaatcacaa atcgatgcct tagaaagcca agtttctgaa atcaacacac aagcacaaga
    13681 cctattagca aaacaagata cattgcgtca agaatctgca caattagtaa aagacattgc
    13741 agatttacaa gaacgtattg aaaaacgtga agatacaatc caaaaacaag cacgcgaagc
    13801 acaagtaagc aacacaagct caaactacat cgatgctgta ttgaatgctg attcattagc
    13861 agatgctatt ggacgcgttc aagcaatgac aacgatggta aaagcaaaca acgacttgat
    13921 ggaacaacaa aaacaagata aaaaagctgt agaagacaaa aaagctgaaa acgatgctaa
    13981 actaaaagaa ttagctgaaa accaagctgc tttggaatct caaaaaggcg atcttttatc
    14041 taaacaagct gatttgaacg tactgaaaac ttcattagct gctgaacaag caactgctga
    14101 agacaagaaa gctgacttga accgtcaaaa agcagaagct gaagcagagc aagctcgtat
    14161 tcgtgaacaa caacgtctag cagaacaagc tcgtcaacaa gctgcacaag aaaaagctga
    14221 aaaagaagca cgcgaacaag cagaggctga agcacaagct actcaagctt catctactgc
    14281 acaaagctca gcaacagaag aatcatcagc tacacaatct tcaatgactg aagaatcatc
    14341 atcagctaca caatcttcag caacagaaga atcaacaaca cctgaatctt caacagaaga
    14401 atcaacagcg cctgaatctt cagcaacaga agaatcaaca acagcgcctg aatcttcagc
    14461 aacagaagaa tcaacaacag tgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc
    14521 tgaatcttca acaacagaag aatcaacaac acctgcgcct acaacaccat caacagatca
    14581 aagtgttgat acaggaaacg gcacaggaag tagtactccg gctccaacgc caacaccaac
    14641 acctgaacaa ccaaaacctg taacacctgc tccagctcct tctggaagtg tgaatggtgc
    14701 agcaatcgta gcagaagctt acaaatatat tggtactcca tatgtttggg gcggtaaaga
    14761 tccaagtggc tttgactgct cagggttcac acgctatgtt tacatgcaag taactggtcg
    14821 tgacatcggt ggttggacag ttcctcaaga atcagctgga acaaaaattt cagtttctca
    14881 agcaaaagct ggagacttgt tattctgggg gtcacaaggc ggaacttacc acgtagcgat
    14941 tgctttaggc ggaggacaat atatccatgc tcctcaacca ggcgaaagcg taaaagttgg
    15001 ttcagttcaa tggtttgccc ctgactttgc tgtcagcatg taagaatgat taatcaaaaa
    15061 gagttagccc attctggact aactcttttt ttattgtgat aaaatagaaa tcagataaat
    15121 tcttctgtca attccatgaa cgtttggata tcttcagtca ccatgtcgat acctgcttgc
    15181 caaaaatctg gttgggtaag atcgacattc aaatgtttct tcgcaagatc ttccgtcgtc
    15241 attgaggcag tatcgcgtag taaatggata tattcttctt caaaatttgc tcccttttgg
    15301 ttggcgtaag catagatgcc caaactaaag aggtagccaa atgtgtatgg gaagttataa
    15361 aacggtacgt catcaataaa gaaatgcaat tttgcagccc agaaatatgg atggtaagta
    15421 gctaaagctc cttgatagcc ttctttttgc gcctcaacca tcatctctgt gatcttatct
    15481 tctgagacta atccttcttg ccgtgcttca tagaatcgat tttcaaaaat gaatcgggaa
    15541 tggatattca tgaacatagc taaagcattt tgcagttttg tatctagcaa attgattttt
    15601 tcttcttttg tttttgcgac ttttaatgta gcatcggcta cgatcagttc tgcgaatgta
    15661 cttgctgttt ctgcgacatt catggcatat tcgcgattta aagaaggtaa atcccacatc
    15721 gtgctgctat ggaaagcatg tcctagttca tgagcaagtg tcgcaacctc attaacagaa
    15781 ttactgtagg tcataaagat ccttgattct tttgtttctg gtaactcagt acaataacca
    15841 ccaggacgtt tcccaggacg atcttctgct tcgatccagc tcttttcaaa agcagattgg
    15901 gcaaatttag ccatttttgg actgaatttg ttaaagtttt cgataataaa tgctgctgct
    15961 tcgtcaaaac taaaggtttt ttctttcaaa tcgcctaaaa taataggtgc atcttgatct
    16021 tgccattcca ttttttcttt accgaaaaga ttagcttttc tagtcaagta attaacaagg
    16081 ggttgtttgt ttttctggat tgtatcccac atgacagaca atgtttcttt cttcaaccga
    16141 ttgtactcta atggtttttg tagaaaatct gtgacaccat gaagttcata gtcagaaagt
    16201 ctaaaaccat ctaaatgatt caaagtatcc gtgaagaggg gtgccttttc tttccaggct
    16261 tcttcccatg ctgcgaaaag tgtttctctg acttttggat ctggatcgcc catcatctta
    16321 ttgaaggctt gtccagcaga taattcgact acttgtccat cttgttcgaa gggaatcgaa
    16381 atgctggcta caatcgtatc ataatgactg ctccaagcat ttagaccatc taaagaaagc
    16441 gtatttataa tgttttcttc agcttctgat aataattgtg agccatcacg acgaatctcg
    16501 tttaaacgaa aagcaattgt ttcaaacgaa gattgagaaa gcatctgatt ccaatcattg
    16561 tcagatattt ctgcaaattt ctttgacaat acagtttcgg ctgattgcag acgaggcagc
    16621 attgcgtaaa gcttgcctga taagaccttt gcatctgaat cattgacatt ggcagaaagc
    16681 aatgcagtaa tatagctgct gcattgggta aaaccattcg taatcgtttc ttgcaattga
    16741 agaatagcgt ttaactgctc tgcattgtca gaactaggtg accattttgt tacgcggtga
    16801 taatactcgt tcatttggtc ttccaattgc ttcatccgtt ggttcaaagc atcagaatga
    16861 ctgcctccgg aaaaaataga atctagattc caatttaatg aataactcat gaaaaaactc
    16921 ccttcatatt gtaatacaaa attaagtata gcactttttt atggaagaag tctctatgaa
    16981 aatgattgtt gtttcttcaa aaaaagtcta agataaaata cagaggtgac ttatgaattc
    17041 ttcaaaaaaa caaatgaaaa acggaaatat ttatttagta atcgcactga tagtcgttat
    17101 aatcttattt tacagttctt ctcaaactta cggacagcaa tcacaagttt cgcgattaga
    17161 aagctggctg cctaatcagc catttaaaga cacattagcg ggtatacgtt ttaattatgg
    17221 tggaagtatg gtgagtatcg accatctggg ctacgccaaa tttttagagt tctttttaag
    17281 gaagggcgca catttcggta cctattttat tttgggaggc agcttgtttt taggtgtttt
    17341 ccccaagcta aaaatttggt ggctgacagc gatccttgct tggctttcag caacagggta
    17401 cgcaggtttg gacgaattcc atcagatgat gaccggtgga cgaacaccta tgttccaaga
    17461 tgtcatgttg gattcgatcg gtgctctgac tgctgtgata ttatgtgtgc tttttgcggc
    17521 attcaagaag cgtttataga ttggctgtgg ctttgttttt ttgtgaaata gtactagaat
    17581 agaagtgtat tctcaaaaga acaagggagg aaaaatcatg tcaggacata gtaaatggca
    17641 taatatccaa ggaagaaaaa atgcccaaga tgcaaaacga gggaaagtct ttcagaaact
    17701 ttctcgtgaa atttatatgg cagctaaagc aggcggtcct gatccatcta tgaatccagc
    17761 tttgcggctt gttgtggaca aagcaaaagc tgcaaatatg cctaacgaca atgtggaacg
    17821 ggcgattaaa aaggctacga gtgccgtaga tgaaacgaat tacgatgaaa tcacgtatga
    17881 aggatatggt ccgggtggag taggaatctt agtccatgca ttgacagata accgtaatcg
    17941 gacagcgaca aatgtccgag tagcttttac tcgaaacggt ggatcactgg gagaaacggg
    18001 ttcagtcagt tacttgtttg accgcaaagg atatatcgct attgaacgcg atgggttgac
    18061 tgttgatgaa gagacgatgt ttgaggatgt attagaggcg ggggcagaag atctgcaaac
    18121 ttctcctgaa gtattcgaga tttatactgc accagaagat tttgctgctg tacgagatga
    18181 gttggaaaaa gagttcactt tggcgcaagc agaattgacg atgattccac aaacgacgat
    18241 tgatttaaat gaggaacaaa aagagcaatt ggaacgtcta gtagataagc tagaagacga
    18301 tgatgatgtt tcagaagtct ttactgctgc tgaaatgtaa gtagcacatt gttagataat
    18361 aaaaagaaag cacctgtacc aatcgtacgg gtgctttctt tttata
//
LOCUS       DBNIBO010000040        18296 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.043, whole genome shotgun sequence.
ACCESSION   DBNIBO010000040 DBNIBO010000000
VERSION     DBNIBO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.043"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            82..2271
                     /locus_tag="AC1Y77_001201"
     CDS             82..2271
                     /locus_tag="AC1Y77_001201"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease"
                     /protein_id="HIQ1574791.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2268..4061
                     /locus_tag="AC1Y77_001202"
     CDS             2268..4061
                     /locus_tag="AC1Y77_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIQ1574792.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4261..4575
                     /locus_tag="AC1Y77_001203"
     CDS             4261..4575
                     /locus_tag="AC1Y77_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HIQ1574793.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4596..4973
                     /locus_tag="AC1Y77_001204"
     CDS             4596..4973
                     /locus_tag="AC1Y77_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HIQ1574794.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            4983..5756
                     /locus_tag="AC1Y77_001205"
     CDS             4983..5756
                     /locus_tag="AC1Y77_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574795.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5778..7181
                     /locus_tag="AC1Y77_001206"
     CDS             5778..7181
                     /locus_tag="AC1Y77_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIQ1574796.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7363..8547
                     /gene="mobT"
                     /locus_tag="AC1Y77_001207"
     CDS             7363..8547
                     /gene="mobT"
                     /locus_tag="AC1Y77_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HIQ1574797.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8544..8834
                     /locus_tag="AC1Y77_001208"
     CDS             8544..8834
                     /locus_tag="AC1Y77_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574798.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8831..9052
                     /locus_tag="AC1Y77_001209"
     CDS             8831..9052
                     /locus_tag="AC1Y77_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574799.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9112..9873
                     /locus_tag="AC1Y77_001210"
     CDS             9112..9873
                     /locus_tag="AC1Y77_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HIQ1574800.1"
                     /translation="MFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISEVTLDET
                     DDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDT
                     ILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEIL
                     KKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRH
                     SLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            9934..10434
                     /locus_tag="AC1Y77_001211"
     CDS             9934..10434
                     /locus_tag="AC1Y77_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HIQ1574801.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            10490..11128
                     /locus_tag="AC1Y77_001212"
     CDS             10490..11128
                     /locus_tag="AC1Y77_001212"
                     /inference="COORDINATES: protein motif:HMM:NF039331.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HIQ1574802.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11200..11595
                     /locus_tag="AC1Y77_001213"
     CDS             11200..11595
                     /locus_tag="AC1Y77_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1574803.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11579..14032
                     /locus_tag="AC1Y77_001214"
     CDS             11579..14032
                     /locus_tag="AC1Y77_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1574804.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14029..16056
                     /locus_tag="AC1Y77_001215"
     CDS             14029..16056
                     /locus_tag="AC1Y77_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HIQ1574805.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16053..17075
                     /locus_tag="AC1Y77_001216"
     CDS             16053..17075
                     /locus_tag="AC1Y77_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIQ1574806.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17092..18021
                     /locus_tag="AC1Y77_001217"
     CDS             17092..18021
                     /locus_tag="AC1Y77_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HIQ1574807.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         6354 a         2935 c         3506 g         5501 t
ORIGIN      
        1 tataatgaga acttttacta acataattta tgataaactt actatataat catattaatt
       61 aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa ttttagaaat
      121 tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg tgagaatgat
      181 tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag ttattactat
      241 agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg gtggcaaggg
      301 cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt tgacaatgag
      361 atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga attgatttca
      421 aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa agctattaga
      481 aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga atttagaaat
      541 tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct aaatttctgt
      601 atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc tagtaatcct
      661 gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat tttcaagcat
      721 atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa aaacaatcga
      781 aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag
      841 tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc tgaaattagg
      901 aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt gtattctcct
      961 aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa atttctttca
     1021 atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct aaatatgatt
     1081 tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact acttaatatt
     1141 atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac tctgttcgaa
     1201 ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc tgtacattta
     1261 gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg caaatctatc
     1321 gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa aaagaacaat
     1381 ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg attgcttttt
     1441 tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc tcaaatagta
     1501 aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa tgttggtagt
     1561 acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag aaagtgtgct
     1621 attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta taaaagcact
     1681 gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa tgaaaacaat
     1741 tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga tgtgaataaa
     1801 gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga taaaaaaaca
     1861 ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa tttagcgatg
     1921 ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg tgaaaaaatt
     1981 gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc atgtcaggaa
     2041 atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt gtacgaggaa
     2101 actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc tgatttgaat
     2161 atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt gtatctaatc
     2221 aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg acaaatatct
     2281 tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata
     2341 acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg
     2401 cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta
     2461 atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta
     2521 atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat ccttttaaaa
     2581 tgaatctctc aagagtatca aaaggattta caattattga tgattatact caaagagctt
     2641 acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa
     2701 ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact caagtttctg
     2761 aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg attttactag
     2821 tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct agtaatataa
     2881 gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat gtaatagcta
     2941 aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt gaccaagcaa
     3001 tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg ctaactagtc
     3061 acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga ttagtagact
     3121 tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat aataaaaacg
     3181 accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc aacataataa
     3241 aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt tgtgtacttg
     3301 ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt
     3361 gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt ttatttttca
     3421 agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga aagctaattg
     3481 caaacgatat tttaattata ttaaaaaatg aatttggaat agaagttaaa gattatttta
     3541 ttgacttaga tttattacag ctaataaaca gtgtgaaaag aatgtatagt ggagacgaca
     3601 acggtgttga tttatacaaa tttgttacca aacatatatt tgttaatctt aatattgacg
     3661 agaaaaatta cccgtctatt tttaagcact atctattttt tattaacgaa gtagaagatc
     3721 ggataaaaaa taattcatta tctagtagtg tatctagttt taagaagatg tttaaatctc
     3781 gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat gatactgtta
     3841 tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt aatgtttctg
     3901 ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga gcaaaaaata
     3961 atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg ctttatccca
     4021 ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag gtagtcgttg
     4081 ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt acccaatttt
     4141 gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt
     4201 taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat aggtaaaaat
     4261 atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt agaatatgct
     4321 ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct ctctcgtagt
     4381 tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct tccctccgaa
     4441 gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc acatattacc
     4501 gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca cgcagacgat
     4561 atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa ggtattgtga
     4621 ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt gaggttcgta
     4681 tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat gacttaaaat
     4741 caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct ttgaaagaat
     4801 ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta gcgacggcta
     4861 cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta ttaaaaggac
     4921 agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc
     4981 ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc atttctttta
     5041 ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt ctagggattt
     5101 tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa
     5161 ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta gaaattggaa
     5221 agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa gctctttcag
     5281 ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca ctggcagatg
     5341 gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac tgcgggtatt
     5401 tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct cgaagtgctc
     5461 gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat tggctttccc
     5521 aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt cgctatctct
     5581 atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga cgtcattatt
     5641 atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca
     5701 tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta ttttgatagg
     5761 tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc aaagccaacg
     5821 ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt ctagtcgtcc
     5881 tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt cagttagata
     5941 catttttcta tcaacttcat acgccatact tttggagcag tattctcact gctctttttg
     6001 tatgcggact tatcagctgg ctttattatc actatcgtat agattctata aaacagttag
     6061 agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa
     6121 ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc
     6181 attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat gtagaaatca
     6241 ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa acaggattgt
     6301 attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc ctactttatg
     6361 atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat
     6421 tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt gctgggggca
     6481 ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg catacaaatg
     6541 caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg gcggtcatga
     6601 ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt tacgaagata
     6661 tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga gaaaattatg
     6721 cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca atgatggata
     6781 tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta atgttaggac
     6841 gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa tattttagtg
     6901 atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct gaaatgggct
     6961 ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc atcaagggaa
     7021 gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa
     7081 aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa tctgagcctg
     7141 taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg cgttagccac
     7201 gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga aaacaggcaa
     7261 ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag gtgtaaactc
     7321 aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt
     7381 tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa taaactggcg
     7441 gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa ggcagtccct
     7501 actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc cgataaccca
     7561 ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt ggctcaaatt
     7621 atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt tggctattat
     7681 tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca tgatgtaagt
     7741 aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg
     7801 gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa aggtattttc
     7861 aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc agatttggca
     7921 aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt
     7981 ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta tattggctca
     8041 ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata cgtgaaaaat
     8101 gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt aaaaaatgac
     8161 cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa aacagccttt
     8221 gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg tcggagtgat
     8281 tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc attacgccta
     8341 accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca tcatcaagtc
     8401 gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac cattatctcc
     8461 accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga acaacaacat
     8521 ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag aagttagggt
     8581 ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat acgtctctca
     8641 ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt tacaaggtga
     8701 gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta accccttttc
     8761 aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt aaggaaagga
     8821 ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata atgcacagtc
     8881 attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac gagaattttc
     8941 aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc
     9001 tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt aacagatatt
     9061 tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa tatgtttaca
     9121 aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc ttcaaattat
     9181 tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac tttagatgag
     9241 actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat
     9301 cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt
     9361 acccagacag caagctccat atatgataaa caaggtgata atgtattagt ggatactatt
     9421 ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat
     9481 gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt
     9541 gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat
     9601 gaaattacag ttcctagaga cgaaaatcta tctaaattat atagtaggat acaacaatta
     9661 attgaaaaag aaattcaacc tactgaactt gctgatatag tgaagacaac gataaggtca
     9721 tcaaatggaa tgattagctc tcttaatgaa gcaagaaata ggcattcact tgcacaccct
     9781 aatacaaata ttattggaaa gagagaagca aaactaataa taggtatttc aagtacagtt
     9841 acagattata ttagcggata ccttgataag tagcgtcatt aactgattgc tacttttttt
     9901 atgactattt ttataaaaag gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat
     9961 ttaggacggt ataatgaagg tgaattagtc ggagcatggt ttactccacc aattgatgag
    10021 gaagaaatgg cagaaagaat cggtttaaat gaggactacg aagaatatgc aatacatgat
    10081 tttgagcttc cttttgatgt ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt
    10141 gaagccattc aagaaatcga aggtacacct atttataatg aactcaaaga aattcaaggc
    10201 atgtggttta gtagcttgga agaattactg gaaaacaaag aggatattca ttgttatagt
    10261 gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg acagcttggt
    10321 gaagtccctt ccaatttaca aaattatatt gactaccaat ctttgggacg tgatatggaa
    10381 atagagggta attacctagt tacctctcat ggggtatttg aatattgtca gtaagtttaa
    10441 caatccgtgt tataattaaa ttaataaata gcacggagag gtataaatta tggtaaatgt
    10501 atttgaaaat cttaaactat taaaactaga catgaaagat aaaggctggg tgatagattc
    10561 cttttacttt cgttacaaac agcaaaatta tattgtactt gttaagctat ttgaaaaaga
    10621 ggaaaaagtt ccagaatatg ctctgttaaa actagaattt ttaaaggaaa acgatttttc
    10681 agatatgcta gctgtctatg ctaattcggt aaaattatat acagacacga aaacaataag
    10741 agaatatttt ggtatcgaat acagtagtaa cttgggagat gttttatttc aatttagcca
    10801 aactcttgct cgatttattc cgacagaagt tagtgagaag aaaaatgaag accaaaaaga
    10861 agccatgtgt ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa aatattgttt
    10921 ttcagttaga agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa
    10981 tgacaataaa acgagattgt atagacccag cttatatgaa cgattagggt cagataccaa
    11041 tttaagtttc agatattcaa tgaatccaga tgatgaagaa actgatgaag gtattattgc
    11101 aaaatggaca aaaaataaaa ttgaatagtt aaagcattga aagagcaatc acctattttg
    11161 attgttcttt ttctattttt acggaaagga cgtgaagaca tgaaaaaaat aagaagttac
    11221 accagtattt ggtcagtaga aaaagtgatt tatgcgatta atgatttaca actccctttc
    11281 ccaattacct ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt catactcttt
    11341 ggcagtttac caccactttc tctgattgac ggagcactac tcaaatactt agggattccc
    11401 attgttctca ctgggttcat gtgccaaaag acctttgacg gaaagaaacc ctatggattt
    11461 ctaaagtcta tcattaccta tgctctacgc cctaaagtaa cctacgctgg caaacaaata
    11521 cgattaaaaa aagaaacaat gaatgaatcg attacgattg taaggagtga acaactagat
    11581 ggcatatccg attaaatata tggaagataa tctcgtattt aatcatgatg gcgaagtatt
    11641 cgcttattat gaattaattc cctataatta cagtttttta agttccgatg aaaaaattca
    11701 agttcatgat aatttctgtc aattgattgc ccaaaatcgt gatggcaaga ttcatgctct
    11761 gcaactagca accgaatcaa gtattcgagc cacacaggaa caatcgaaaa aagaaatcac
    11821 tggacgatta aaagagatag cttacgaaaa aatagatgaa cagaccgaag cgttaatttc
    11881 tatgattggt gacaatcaag tagactatcg cttttttatt ggttttaaac tctacctcaa
    11941 tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt
    12001 ctttcatggt gtgaatcaca aattaatggg cgattttgtt tctatgccta tggcggaaat
    12061 cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa ttatccagac gctttcagat
    12121 tcgtaaattg gagaaagatg attttggtta tattattgaa catctttacg ggcaaacagg
    12181 tgtccctttt gatgactacg actaccattt accgaaacga tacgaagata aaaaagtgtt
    12241 ggtaaagaaa tacgatattc tcaaacccac tcgctgttta atcgaagaaa atcaacgcta
    12301 tttacggatt gaacaggaag ataccactac ttatgtggcg tattttacaa ttaatagtat
    12361 tgttggtgaa ttggatttcc ctaatagtga aattttttat taccagcaac agcagtttac
    12421 tttccccgtt gataccagta tgaatgttga aattgtcgcc aataaaaaag ccttatctac
    12481 cgttcgcaat aaaaagaaag aactgaaaga tttagatagc cacgcatggg aaagcgacaa
    12541 cgaaaccagt actaatgtgg tggacgcttt agaatctgtc gatgaattag aaagtaattt
    12601 agaccaaacc aaagaatcta tgtacaaact aagctatgtg gtacgggtat cagcaaagaa
    12661 tttggacgag ctgaaacaac gttgtgatga agtaaaagat ttttatgatg atctgtctat
    12721 caaattagta cgaccttttg gcgatatgct aggcttgcat aatgaattta ttccctcaag
    12781 caagcgatat atcaatgact atattcaata tgtcacgtcg gattttttgg ctggtcttgg
    12841 tttcggtgct acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga
    12901 tacaggcaga aatatttatc tccaacctag ccttgcaagt caaggaatta aaggctctgt
    12961 aacaaatgca ttagcgtccg cttttattgg ttcattaggt ggcggaaaat cattctctaa
    13021 caatatgctg gtctattatt cagtactctt tggcggacag gcaaccattg ttgacccaaa
    13081 agctgaacgt ggtgaatgga aagaaacctt acctgatatt gcccatgaaa tcaatattgt
    13141 taatctcacg agtgagaacg acaataaagg gttacttgac ccttatgtga ttttaaaact
    13201 cccgaaagat tcagaaagcc ttgccattga tattctcacg ttcttaacag gtatttccag
    13261 ccgagatagc gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa
    13321 acaacgaggg ttgttattgg tgattgatga attgcgaaaa gaagatacgc ccatcagcaa
    13381 tagtattgct gaccatatcg aaagctttgt cgattatgat tttgcccatc tgttattcag
    13441 tgatggcagt gtcacccaat ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc
    13501 agatttggtc ttacccgatt ccaatagctc ttttgaagaa tacactacca tggaattatt
    13561 aagtgtggca atgctgattg tcattagtac ctttgcttta gactttatcc actctgaccg
    13621 ttccattttt aaaattgttg accttgatga ggcttggagt tttttacaag tggcacaagg
    13681 aaaagctcta tctatgcgat tggttcgagc aggacgtgcc atgaatgcag gggtctattt
    13741 cgttactcaa aacgcggacg atttactaga tgaaaagatg aagaataata ttggcttgaa
    13801 atttgccttt cgttccactg acctagtgga aatcaaaaag accttagaat tttttggtgt
    13861 ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt
    13921 agtttcagac atctatggtc gtgtaggggt tatgcagttt cacccgattt ttgaagagtt
    13981 gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa
    14041 caaaagaaat ttcttcacta tagttggata acgctccttt gtgtgggcgt tattttactt
    14101 ttattaggca cgctgggcaa tgcagtacaa gcgacaggct tagttgatga aaccattgat
    14161 acaagcaatg aatactccaa gtatggtttg aatcaatatc agcttgatta ttatgtggac
    14221 aatagctggg gctggcttcc atggaattgg agtgatggca taggacaatc ggtgatgtat
    14281 ggattgtatg ccattactaa ttttatttgg acaatttctc tttatctatc gaacgccaca
    14341 ggttatttgg tgaaagaagc ctatgcctta gatttcatta gtcaaacatc ccaagcaatt
    14401 ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg gcttttccag ttcagggttt
    14461 tatgtcggtt tcctccttct ttttattctc attgttggaa tctatgttgc ctatacagga
    14521 ttggttaagc gagaaacgac gaaagtgata cgagctgttt taaactttgt cgtggtgttt
    14581 attctatcgg cttcctttat tgcgtatgca cccgattata ttgcgaaaat caatgatttt
    14641 tccaaagatg tcagtaccgc cagtctgaat attgggacaa aaattgttct tcctaacacg
    14701 gatagtcaag ggaaagacag tgtggacatg atacgtgaca gcttattctc tgttcaagta
    14761 aagcaaccat ggctattgct tcaatatggc tccactgatg tagaagcgtt aggggaagaa
    14821 cgtgtggaaa gtttattgtc taccagtcca gatacaaaca atggtgaaga tagagaaaat
    14881 gtagtgattg aagaaattga agatcatgac aatacctatt taaccctacc gaagacgatt
    14941 tccagattag gaacagtctt ctttttgttt gtattcaata ttggtatctc gatttttgtt
    15001 ttccttctaa caggaatcat gattttttcg caagtgctat ttattatttt cgctatgttt
    15061 ttgccaatca gtttcctatt aagtatgttg ccgagttttg atggcatggg caggcatgcc
    15121 attaccaagt tatttaatgt gattatgaca agggctggta ttaccttgat tattaccgtt
    15181 gcctttagta tttccactat gctttattcg ctgtcagcaa gttctccctt tttcatgatt
    15241 atgtttttac aaattgtgac ctttgcaggg atttatttta aactgggcga tttaatgagc
    15301 ctattcactt tacaaagcaa cgactctcaa aacatgggca gacaagtgtt tcgcagacct
    15361 cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa gatttttcgt
    15421 ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca aaccaataaa
    15481 gctaaccata ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc actttccaat
    15541 gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc ccatgtattg
    15601 gacacgaaaa atcgtctagt caataaaaga aagcaggcaa aacaacagat acaggcgaca
    15661 ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca taatgtaaca
    15721 gactttaaac aatctatggc agataccaaa gctcaacgca aacaacagcg tatgaaccag
    15781 caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg agctggataa agcaaaagaa
    15841 aaaagacgaa aagaaaaccc atcatctcct gttcagaata accagcagca tgttcgcaag
    15901 cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa tcatcagcga
    15961 ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc aattcaatca gaggaagcta
    16021 aataaaagaa cggataagcg aggacaacaa acatgaagct aaaaataaca gtgatttgta
    16081 gtgtggtctt ctctctcttc ttttttctct tattattcct agtcattttc tttgcagatg
    16141 atacggacag tggcgaaaac aacaaggatt cttctattcc acaaggcggt gtgaccgttt
    16201 caccagaagt gttggcacac cgtccgttga ttgaaaaata tggtaaggaa tatgggattg
    16261 aagactatgt ttcttatatc ttagccatta tgcaagtgga atcgggcggt acagcagaag
    16321 atgttatgca aagttccgaa tctttgggtt taccgcccaa cagtttgagt accgaagagt
    16381 ccatcaaaca aggggttaaa tatttcagtg aattattaac cagtgcagaa caacaaggag
    16441 tggatataga ttctgttatc caatcttata attatggcgg tggcttttta aattatgtga
    16501 ggagtcatgg gaaaaaatat acctatgaat tagccgaaca gttttctaaa gagaagtcgg
    16561 gtggtcaaaa agcagactat cccaatccta tagccatacc tgtgaatggt ggttggcgat
    16621 ataactatgg aaatcagttt tatgttcagt tggtttcaca atacctaacc gacacttccc
    16681 caacagaatt tgatgatgaa accgtgcaag tcattatgga cgaagccttg aaatacgaag
    16741 ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa
    16801 tacaatgggt gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa gatcagtatg
    16861 acgctacgca agaaatctct atggaagaag cccaagcagg cgaccttatc ttctttcatt
    16921 caacctataa tgcaggcaca tatgtgactc atgtggcgat atatttagag ggaaatcgct
    16981 tttatcatgc aggcgaccca atcggttatg gcgacttaag cagtcgttat tggcaagacc
    17041 atctgattgg ggctagacgt gttattcata actaagaaag gaatgaatca aatgattata
    17101 aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa
    17161 agaaaagttc cgatgattaa agttggcacg cataagaaat taacctttgt tttatgggtt
    17221 ttattgattg gtagtgtcgg atttggaatc tataaaaatt ttactgccat tgacactcat
    17281 actgtccgtg aaacagaaat cattaaacag cagattgtcg ataccaacca agtagaaagt
    17341 tttgtgaaat cctttgcgaa agactacttt tcgtggcaac aatcccaaga agccattgat
    17401 aaacgaaatg aaaaattgac ccattatttg acggaagaat tgcaggtatt aaatgaagaa
    17461 atgattcgta aggatattcc tactagttct agtgtgaacg atatacaggt gtggcaggtt
    17521 tctcaagtga atgagaacac ctttgaggta ctgttttctg ttgaacaagt tattaccgaa
    17581 gacaaagaca aggaaactat atcgtctagc tttcatgtgg ttgtccatat agatgaatca
    17641 gataatatgg ttattatcaa aaatccgaca atgagtaaga aacctcaaaa atcggactac
    17701 caacccaaac agctagaaag tgaccataca gtagatacag aaacgatgga cgaaatcact
    17761 agctttttag aaacgttctt ccagctctat cctaccgcca cagaaaaaga attgacttac
    17821 tatgtaagta atcatgtgtt acctatgatt aacaaggaat atgttttcga ggaattggtg
    17881 aatcctatct ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt gaaatattta
    17941 gatcaagaaa caaaggcaac ccaaatctcg caatttgagt tgatattaga aaaacaagat
    18001 aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg
    18061 tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt
    18121 taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
    18181 aatttgatag cgggaacaaa taattagatg tcctttttta ggagggctta gttttttgta
    18241 cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata tctgta
//
LOCUS       DBNIBO010000041        18235 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.044, whole genome shotgun sequence.
ACCESSION   DBNIBO010000041 DBNIBO010000000
VERSION     DBNIBO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.044"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(31..708)
                     /gene="phoU"
                     /locus_tag="AC1Y77_001218"
     CDS             complement(31..708)
                     /gene="phoU"
                     /locus_tag="AC1Y77_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIQ1574808.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(721..1479)
                     /gene="pstB"
                     /locus_tag="AC1Y77_001219"
     CDS             complement(721..1479)
                     /gene="pstB"
                     /locus_tag="AC1Y77_001219"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIQ1574809.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1495..2301)
                     /gene="pstB"
                     /locus_tag="AC1Y77_001220"
     CDS             complement(1495..2301)
                     /gene="pstB"
                     /locus_tag="AC1Y77_001220"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIQ1574810.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2316..3200)
                     /gene="pstA"
                     /locus_tag="AC1Y77_001221"
     CDS             complement(2316..3200)
                     /gene="pstA"
                     /locus_tag="AC1Y77_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIQ1574811.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3200..4120)
                     /gene="pstC"
                     /locus_tag="AC1Y77_001222"
     CDS             complement(3200..4120)
                     /gene="pstC"
                     /locus_tag="AC1Y77_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIQ1574812.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4235..5092)
                     /locus_tag="AC1Y77_001223"
     CDS             complement(4235..5092)
                     /locus_tag="AC1Y77_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HIQ1574813.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5429..6877)
                     /gene="cls"
                     /locus_tag="AC1Y77_001224"
     CDS             complement(5429..6877)
                     /gene="cls"
                     /locus_tag="AC1Y77_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIQ1574814.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            complement(7029..7913)
                     /gene="ftsX"
                     /locus_tag="AC1Y77_001225"
     CDS             complement(7029..7913)
                     /gene="ftsX"
                     /locus_tag="AC1Y77_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIQ1574815.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(7906..8592)
                     /gene="ftsE"
                     /locus_tag="AC1Y77_001226"
     CDS             complement(7906..8592)
                     /gene="ftsE"
                     /locus_tag="AC1Y77_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIQ1574816.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(8697..9798)
                     /gene="prfB"
                     /locus_tag="AC1Y77_001227"
     CDS             complement(join(8697..9725,9727..9798))
                     /gene="prfB"
                     /locus_tag="AC1Y77_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIQ1574817.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(9925..12459)
                     /gene="secA"
                     /locus_tag="AC1Y77_001228"
     CDS             complement(9925..12459)
                     /gene="secA"
                     /locus_tag="AC1Y77_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIQ1574818.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            complement(12681..13232)
                     /gene="hpf"
                     /locus_tag="AC1Y77_001229"
     CDS             complement(12681..13232)
                     /gene="hpf"
                     /locus_tag="AC1Y77_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIQ1574819.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(13360..14079)
                     /locus_tag="AC1Y77_001230"
     CDS             complement(13360..14079)
                     /locus_tag="AC1Y77_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIQ1574820.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(14076..15389)
                     /locus_tag="AC1Y77_001231"
     CDS             complement(14076..15389)
                     /locus_tag="AC1Y77_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIQ1574821.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(15470..15790)
                     /locus_tag="AC1Y77_001232"
     CDS             complement(15470..15790)
                     /locus_tag="AC1Y77_001232"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1574822.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(15898..16185)
                     /locus_tag="AC1Y77_001233"
     CDS             complement(15898..16185)
                     /locus_tag="AC1Y77_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7662 domain-containing protein"
                     /protein_id="HIQ1574823.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            16312..16956
                     /locus_tag="AC1Y77_001234"
     CDS             16312..16956
                     /locus_tag="AC1Y77_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIQ1574824.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(17016..17723)
                     /locus_tag="AC1Y77_001235"
     CDS             complement(17016..17723)
                     /locus_tag="AC1Y77_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1574825.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(17943..18113)
                     /locus_tag="AC1Y77_001236"
     CDS             complement(17943..18113)
                     /locus_tag="AC1Y77_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574826.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         5066 a         3993 c         3193 g         5983 t
ORIGIN      
        1 ctaaataaac agcagaatca caacttcagt ttagaaaatc tcattgtggt tcgtatttaa
       61 ttcagtgatt ttccctgtag ccaagtagac gatccattca cagatgtttg tcacatagtc
      121 tccgattcgt tctagatagc tggcaacatt caaatagtct gtgccactga tgactgtttc
      181 aggattttct tgcatgtttt tgatggttgc attatagatt cgattgaaat agtcattgac
      241 tctttgatcc atattggcga ttgtacgcgc atctttttcg tctgttttga catacgcaac
      301 taggacgttg tccaccattt ttttcacgta atcggacata tcggagatct ctttttcgat
      361 ttccggtatt ctcgtctgtc cttttacgcg gattgtggat ttagcaacgg acaccgcatg
      421 atcggccata cgttctaaat cagagctagc tttcatgacc gtgataatca tacgcaagtc
      481 agttgtaact ggctgttgta atgcaatcat ttcaaaactt ttcttctcca aacgcgtttc
      541 catattattg atatttatat cgttttcgat aacttcttga gctaagattt tatcgtgttt
      601 gatgtatgca cgaactgact tgtgcacagc gctgctcacc atcatcccca tttcgtagaa
      661 ctgattatgg agattcagca attcttcttc aaattgtgta cgtagcattc cgtttcctcc
      721 ttatccaaat cgacctgaaa tataatcttc tgtctcttgt ttttctggat tcatgaatat
      781 cttcttcgta tcgttgtatt cgatcaagtg accatctaaa aagaatgccg tcttatctga
      841 aatacgtgaa gcttgagaca tattatgggt cacgatgatc atcgtatacc gctctcttaa
      901 ttctaacagc atactttcga ttttcccagt agaaacaggg tcaagtgcac ttgttggttc
      961 gtccaataag atgatttcgg gattcactgc caaaacacgt gcgatacata cacgctgctg
     1021 ctgcccaccg gataaagaca aggcactttt atgcagttta tctttcacat cctcccaaac
     1081 ggacgctgcc ttcagacttt cttccactac ttgatcaagg acttttttat ctttttcacc
     1141 ttttaacttc aatccataga tgacattttc ataaatagaa aaaggaaatg gatttggctg
     1201 ttggaacacc atgccgattt cttttcttag ttcaacaaca tctgttttcg gaccatagat
     1261 gtctttgtct ttatataaaa cacttccggt gatcgttaca tttggaatca gatcgttcat
     1321 tcgattcaac gaacgtagat aggtagattt cccgcaacca gaaggaccga tcatcgctgt
     1381 gatttcacct tgattgaaac ttagatcaat tccctttagt gcttctttct ttccataata
     1441 cagatgaaga tctttggatg agataatttc ttttgtcatg ttgaggcctc cgtcttaacc
     1501 aaaatgaccg gacacataat cttcggttgc ctgaattttt ggtctggtaa atatcttgtt
     1561 tgttttgtca tattccatta cttttcctag atagaaaaag gcagtatagt cgctgatacg
     1621 agctgcttgt tgcatattat gcgtcacgat aatgatcgtg tagtcttctt tcaagtttac
     1681 aagtgtttct tcaaccgttc ctgtcgaaat aggatccaag gcacttgccg gctcgtctaa
     1741 cagcaagata tcgggtttca tcgcgattgc acgagcgata cacaaacgtt gctgttgtcc
     1801 gccagataaa gccaaggcac ttttatccaa gctatctttt acttgatccc acaaagctgc
     1861 ttgtttcaag cttgtttcaa cgatttcatc taatttcttt ttgttttttt caccatgctg
     1921 tttcaaagca aaagttatat tgtcataaat cgatttgcta aaagggttgg ggcgttggaa
     1981 caccatgccg atccgtttac gcatttcata aacatccact tgaggcgcat tgatatccac
     2041 gtctttatac ataatctttc cagtaatttt cgtattagca atcccatcat tcatacgatt
     2101 caaagaacga agatacgtgg attttccgca gccagaggga ccaatcaatg cagtgatctt
     2161 atttttttcg aattctagat ccactccctt gattgcttca ttgtcgccat accaaacatg
     2221 cagatcattc gtatataatg caacttcctt atcttttggc atggtaatga catttgtttc
     2281 attccaatta tattttttca ttttacttct tctccttagg ctgaagtcat ttttcggtat
     2341 aatcgtttgc caatcgcacg agcgccgaag ttgaacagta aaacagcaat gatcaacacg
     2401 gcagaagcgc cagccgaaac agaaacacca tcaggcatcg taccttctgt gttgattttc
     2461 caaatatgga cagccagtgt ttcagcttgg cggaaaatgc tgatcggact agaaacactt
     2521 aatggattcc agttcgtaaa atctaatgct ggcgcgcttt gaccagctgt ataaatcaat
     2581 gcggcagctt caccaaaaat tcttcctgaa cttaggatca caccagtcaa aattcctggt
     2641 gtcgcttctg gaacaaccac ttttatcact gtttcccagc gtgacaaacc taacgccaat
     2701 ccagcttctc gttgtgtgta atggaccgct tgtaaagatt cctcgaccgt tcgagtcaaa
     2761 agaggcaagt tgaatacggt caaagctaat gcaccagaaa tgattgagaa accatactgg
     2821 aattgaataa cgaaaaccaa gaaaccgaat aaaccgacaa caacagaagg caaagagctc
     2881 aagatttcga tagacgtacg gatcacgtct gtcagccagt tttttccggc atattccgaa
     2941 aggtagatac ccgcacctaa agagatagga aaactgatga tcatggtaat caaaagcaaa
     3001 taaatcgagt taaataattg aatcccgatc ccaccgcctt cttggtaggc tttggaaggt
     3061 tcagtcaaga atgaccatga aatgtgcggt attcctcgaa ctaagatata aagtaataaa
     3121 gctgctaaga tcaatacgat gatccctgaa accacatata gaacacctgt agcaatttta
     3181 tctgagcgtt ttgcgttcat tatttcagct ctcctttctt cccaatcata cggatcacga
     3241 tattaaagaa taatgacatc aatagcaaga tcagcgctaa tgaccataac acattatttt
     3301 caactgttcc catgattgta tttccgattc ccattgtcaa gatggacgtc aatgtagaag
     3361 caggagtagt aagtcctgtt ggcattaatg ccgcattacc aataaccatt tggatagcta
     3421 aggcttcgcc aaacgcacga gccataccaa aaacgactgc tgttaaaatt ccgggcactg
     3481 ctgctcgcag caccactttg tagattgttt gccatcttgt ggcacctagc gctaatgaag
     3541 cttcccgata gtgtcgcggc actgccttta atgcatccac agtcatcgtt gtgacagtcg
     3601 gtaagatcat aacgaataac acaaatgtac cagctaagat accgaaccct gtcccgccaa
     3661 agatacttct gacaaaaggc acgataacag ataaaccgat aaatccgtaa acgacagacg
     3721 gaatacctac aagtagctcg atgaccggtt gcaagatctt cgttccgttt ttaggagaaa
     3781 tctccgtcat gaacactgct gctccaatag caaatggtgt ggcaacaata gcagataaaa
     3841 aggtcacaat aaatgaacct gtgatcatag gcaatgcccc taccatgggt ttcccatttg
     3901 ctcctatttg gcttggattc caatctgtac caaacagaaa atcaaataca ttgatcttat
     3961 cagtaaaaaa ggtggccaat cctttactag cgacaaaata aaaaatagat aatacaacca
     4021 acacgatcag cgcgatacac aaaaaactaa tgaattttcc gcgctgttcc atttttgaac
     4081 gttttgattt tgttaataat acttttttca catcttccac agatgactcc cccttaatca
     4141 ggtttaacat actcactcag tgtacaaatt gaacatcaat ttggttttaa taacatgtaa
     4201 aagtttagct aagtttgtaa attttgtgta aagattactc tttaacaatg tttccttgcc
     4261 aatcacgttc aactttcata tgtgaaacag ggatatagcc tagctctccg acaatattat
     4321 cttgtacttc atcagataag atatagtcta aaaattcttt tgtcagtttt tttggatggt
     4381 ctcttgtgta catatgctca tatgcccaaa tcgtccattt gttcgttgtc acattttcat
     4441 ctgttggttc cacaccgtta acgttcaacg tatccacatc atcggaaacg taagaaaaag
     4501 caacatagct gattgctccg ggtgtgtccg caacgatttg gcgcaccatt ccgcttgaat
     4561 cttgttcttg tgcctgaaca gctgtttctc cgtccaatac ccatttttca aacgtactac
     4621 gggtcccgct tcctgatgca cggttcaata taactactgg ctgatctttc ccaccgactt
     4681 cactccagtt tttgatctcg cccgtaaata attttttcag gttttccata ctgatatctt
     4741 ttacgccaat atccttgttc acaataggag tgattccaac aactgctact cgatgatcga
     4801 ctaatgccga agcatcaatc cctgattttt cttccgcgaa caaatcagaa tttccgatat
     4861 cgactgctcc tgactgtact tgagacaacc cagttccgct tccgccccct tgtacattga
     4921 tgaattttcc aggattttct gcctggtagg tttcagcaac cgtttctact aaaggctgta
     4981 aggcagaaga gccaactgca gtaatggatt ctcctcgatc aatccacttt gaacagcctg
     5041 ataaaagaat agctaatcct attattggta agaataattt tagttttttc attagaaatc
     5101 tcctttaatc ctaaagtatg tttattctat cattttcatt acttgatgaa aacgaatcaa
     5161 aatcattaaa taacaaaata aattctcata attgcaaaaa aagctatctc attaaaagtt
     5221 tctatgctat actggctttg aaatgtgtct cttttaatag catatattag cttatttctt
     5281 gctttcccca aaaaacaaga gataccccaa agttaatttc gctcatgcga gacgctttct
     5341 ccatttctga cgtactctca gaaatggccc cgagaaaaaa ggacgcgaga tttccatcta
     5401 ttgatggctc tcctgtcctt tttgtatttt aaaggagcgg cgaaatcagt cttgctaatc
     5461 cttctttcat cttgatcatc atccctcggt tttcatacac ttcttttgtg agatgcgtcg
     5521 aaatctttga atcttcaaag aatgcttttc ttacgcgaga agccattcgt tcatcataga
     5581 tcagtgtatt gacttcaaaa tccaatctaa atgagcgaac gtcgatatta gctgaaccaa
     5641 cggaaacgat gccgccatcg ataatcatcg tctttgcatg gataaatcca ttttcatatg
     5701 tttcgattct tgccccatac tcgatcagct ctgcagcgaa agaatacgtt gcccagtaaa
     5761 cgagaagatg atctggtttg ttaggtattt ggatatggac ttttacgcct gagagaagag
     5821 ccaacttcaa tgcctcatga atcgatccat ccggtatgta atacggcgtt tggatcaata
     5881 tctcccgttt ggctaacgaa atcattttca aatatgtcat tttgatttgc tcatgttcgg
     5941 tatctggtcc gcttgtcacg atttgaacag ccatatcacc tgtagaatga atggtcggga
     6001 aaaattccgg tgaatatcct acttcttttg tatgctgaga attccagtcc atcaaaaaac
     6061 ggttttgcaa ggtgtaaacc gcttctccat agatacgcag atgattgtct cgccaataac
     6121 caaatttctt taccaatcct agatattcgt ttccaacatt gaacccacct gtatatccga
     6181 ttttcccatc aatcactacg attttacgat gattgcgata attgattcga ggattgatat
     6241 acggaacgaa tagaggaaag aaaaatgcga cttctccacc tgcttttttc aaattctgaa
     6301 aaaaactaga agaaacttgt gtggaacccc atgcatcaag aaggactctg acttttactc
     6361 cgcgtcttgc tgcctctgtc aatgcctctc gaacttcttc acctaatgta tcactgcggt
     6421 aaatataata ttggaaatgg atatggtctt ctgcctgttt gatatcctcg atcagcgcat
     6481 cgaatttttc acgaccatct gtaaataaag tgatttcatt tttagtcgta taaagcgtac
     6541 tttcaaatac agtaagcata tagatcaatt gtttcacatc tacttgtcca gtaggcggat
     6601 gaggataaag ccctcgcatc aatgcttgtt tatcttcttc gatttctaga ttcttaccga
     6661 ttttcccttg gatcttcaaa tcaaagattt tctctttgga aatccctctt cctaaaaaaa
     6721 tatataaaat aaatcctaca actggaatga acatcaaaac gagcagccat gcccatgttt
     6781 gcgcagtttc ctttctttct cgaaaaacga taataaagga aagcaaaata ttcataatca
     6841 aaaggatcgt aaaaaaattg tcaacgataa tcttcacggc ttcacctctt tttctaccaa
     6901 cttaagaata cccaagttgt tttttaaagt aaagagaaaa aagccgcccc atttactcca
     6961 cctagctgat aaaaaaagaa agctggaacg aaatcgtcca actttctgat tatatttatc
     7021 ttgtgacact agaatttcaa gaaacgtctc atagagatga tcgagccgag tgaaccaatg
     7081 atcacaccaa taccgaccat caaaagattg attttccaaa cgatattttc tggatggatc
     7141 aatgaataat gagatcgtaa taatgacgga ttgatcaatt ggtaaaacca tgcgtaacca
     7201 aatgtaatga tcagtatcgg aagaatggca ccgattaagc cgatccaacc accttcaagg
     7261 aaaaatggcc agcggatata tccatttttt gcccctacta aacgcatgat ttgaatttca
     7321 cgctgtctag ataaaatcgt gatccgaatc gtgttagaaa tgagaaacat tgctacaaac
     7381 agcagcaatg ccgccgcaac taatccccat gttcgaactg caccagcaat ttggaaaatc
     7441 cgatctgagg aagccccacc gtaatcagaa cgggaaacat tcttcaaatc tgccgctttt
     7501 tctgctactt gttttgtata agaaggatcg gtagcactta ctacgtatac atcatataac
     7561 gggttattat ccccttcaaa caatttccaa gaatcgccca tttcgttttg gattttcttc
     7621 aattcctgtt ctttgcttga aaattcgact tttttgacat gatctaagtc atttaattct
     7681 ttttctaatt ttttcatctc ttcgtctttt gtaccaatgt ctacgaacac agatacatcc
     7741 acgtttcctt ctatatcttg tgctagtttg gttgcgttca tgatgaccgc catgaacaca
     7801 ccaactaaag tcaacgtgat cgttaccgca ctgaccgaag ctactgtcat ccagccgtta
     7861 cgtttcaaac tcttgatact ttctaataaa tggcggaaaa aggttctaat catcgtatcc
     7921 atattcccct tccgcttggt ctcgaataat gcggccgttt tcgatagcaa ttactcggtg
     7981 tcgaatcgta tttacgatcg tactgttatg tgtagccatc acgacagttg ttccttgtgc
     8041 attgatccga tctaatagtt tcatgatttc ccatgagttt tccggatcta gattccctgt
     8101 cggttcatcc gcgataagta ctttaggtgt atttacgatt gctcgggcaa tagaaacacg
     8161 ttgttgttcc ccaccagata attcgctagg aaagactcgg actttgtgtt tcaaaccaac
     8221 aagatccagc acttccatta ctcgtttttt gatatcccga ggttttctgc cgataacttg
     8281 catggcgtac gcaacatttt catatactgt ttttcttggt aataatttat agtcttggaa
     8341 caccacgccg atttctctgc gtagataagg aacttcgcgg tttttgatgg ctattagatc
     8401 atgacctgcg acgttcaaag ttccttttga agctttttct tctcgataca tcaatttgat
     8461 gaatgtagat ttaccagctc cagacggacc aacaacatac acaaactctc cttgttcgat
     8521 gacaatcgag agattgcgga tagcagtggt accgtttgag tacttcttca ttacatcctt
     8581 catttcaatc atggcgttct ctccatatct ttttcagtct ttagctatta tagcatggca
     8641 atgtttcaac ttgcttgcaa aatcttacaa ttttatttca atttaacaaa gggctcttat
     8701 tgcagatgca ttttcaaata gccgtcaata aataaatcaa gatctccgtc cataacagct
     8761 tggacatttc ctgtctctac gtttgtccga tggtctttta ccatagaata tggatggaaa
     8821 acataagaac ggatctgtga tccccagccg atttcttttt gttctcctct cagtgcagct
     8881 gcttcttgtt ctttcttttc cacttctaat tggtaaagct tagctttcag catccccatc
     8941 gcttgttccc ggtttttcaa ttgtgaacgt tgtgcctgac tcgctactac tgttcctgta
     9001 ggcaagtgag tgattcgtac agcggattct gtcttattga tgtgctgacc acctgcacca
     9061 ctcgcccgat atgtgtcgat cttcaaatca tctggattga tttctatatc gatcgtttca
     9121 tcaagctctg gcatcacatc taccgaacaa aaagaagtat gacgacgttt tgctgagtca
     9181 aaaggcgaga tacgtacaag gcgatgaaca cctttttctg atttcagata gccaaatgcg
     9241 ttgtgtcctt tgatcagtag ggtcacactc ttgattcctg cttcatcgcc tgcttgataa
     9301 tctagtgtct ctacttgata cccatgctgc tctgcccagc gtgtatacat gcgtaaaagc
     9361 atgctgcccc agtcttggga ctccgttccg ccagcccctg gatgcaattc gacgatggcg
     9421 ttgtttcgat cataaggacc atctaacaac atagataatt catacgtatt cattttttca
     9481 tctagcgcat tgatacgtgc ttctgcttct tcttgcatta ctgcatcagg ttcttcctgc
     9541 aacatctcca ccatcaattc gatttcttcc agttcttcag caagagaatg gaattgatca
     9601 taagtttctt tatttgcatt gttttcactg atcacttttt gagcagcttc gctatcatcc
     9661 caaaaaccag gttcagccat ttttgcttct gcttccgcaa tgtcttcttc cagctggtct
     9721 aagtcaaaga gacctcctga agcttgtgac cttttcctgc atttctgtca ggagatttcg
     9781 aatttcggat atttccatgt ttttttcctt ctttcactat acttttttta ttatatctag
     9841 tctaacagaa tcgtcaaata acagaaagaa ggtgagacaa attctttaaa aaagaatttg
     9901 tctcaccaat cctgctgtca gaaattagtt tgcatctttt ccgtgacagt tcttatattt
     9961 tttcccgctt ccacaaggac aaggatcatt tctaccaacc ttgttcacat ggattggttg
    10021 ttttttcgcg ctagtattac tgttttcttt ggcatctcca tcttctgagt gagaagctgt
    10081 tccttgagaa acttgctcac gttgtacgtt ttgacggatc tcagctttca tgaagatacg
    10141 agtgacttca tattcaatgg cacctaccat atcttcgaac atcttataac cttctgtttg
    10201 gtattcgact aatggattgt tttgaccata ggcacgcaag ccgattgact gacgtaattg
    10261 gtccattgca tcaatatgat ctgtccattt agagtccacg acacgtaaga tcacgacttt
    10321 ttcaaattca agcagttgct ctggtccgtt caattgagct gctttggtat tgaatacttc
    10381 ttgtgctcgt tcgtttagat attctttgat ttcttgagag gtttttcctt cgatatctgt
    10441 aagggaaata ctgtcttcat ggacaagcgt gctgccagca aaatcgacga tactttccaa
    10501 attccaattt tctttttcta attgcgtatg cgcatctacc acacgactga ttgtccgttt
    10561 gaccatattc atcagtggtt tagataagct tttttcttcc atgatcactt cttgacgctg
    10621 tttatagatt acttcacgtt gctcacgcat cacgtcatcg tattgcaaga cgttcttacg
    10681 tgtgtcgtag ttgtttcctt ctacgcgttt ttgtgcagct tctacttgtt tcgtcagcat
    10741 cttgctttgg atcacagcat cttcgtcacc gatcttcatt cgatctaaga aagctttgat
    10801 tcgttctgaa ccgaatcgtt tcatcaaatc atcttctaaa gacaagtaga attgtgacat
    10861 accagggtct ccttgacgtc ctgcacgtcc tcgaagctgg ttatcgatac gacgagattc
    10921 atgtcgttct gtcccgataa ctgctaggcc accaagttct cttacgccaa gccctagctt
    10981 gatatctgtt ccccgaccag ccatgttggt agcaatcgtc actgcacctt tttgacccgc
    11041 attcatgatg atttctgctt ctttaaagtg atttttcgca ttcagtactt catgaggaat
    11101 acgttcttga ttcagcatat ctgataataa ctcagatgtt tcaactgcaa cagttccgac
    11161 taagacaggc tgtcctttgc ggtagcgatc tttgatatct tgtacgacag catggaactt
    11221 gctttgtaat gttgggtaaa gcaaatctgg tcgatcgtca cggatgaccg gacggtttgt
    11281 cggaatctgg tatacttgaa tattataaat ctctctgaac tcttcttctt ctgtcttagc
    11341 tgtaccagtc atccccgcaa gtttcttgta catacggaaa tagttctgga aagtgatcgt
    11401 cgccatcgtt ttcgtttcgt cttcgatctc tacgccttct ttagcttcga tcgcttgatg
    11461 aagaccatct gaataacggc gtccatccat gatccgaccc gtaaattggt caacaatcaa
    11521 tacttttcca tcttgaacaa cataatcgat gtctaaaagc atgatgtagt tggctctcaa
    11581 tgcttgatcc aagtgatggg tcaatgcagt attttcaatg tcatataagt tgtccaaacc
    11641 gaatgtttct tcagcttttt cgatccctgc ttcagttaaa ctgattgttt ttgattgtat
    11701 atcgatttta taatcttctt cttctttcaa acgtttcacg aagttgtctg ctcttgtata
    11761 taaagctgtt gatttttctg cttgccctga aatgatcagc ggcgttctcg cttcatcaat
    11821 caagatcgag tcaacttcat cgacaatggc ataattcagc ggacgttgta ccatttgatg
    11881 acggtaaacg accatgttgt ctctcaaata gtcaaatcct aattcattat tcgtgctata
    11941 agtgatatcg caattataag cctcgcgttt ttcatccgaa gattttgagt tgatattcaa
    12001 cccaactgtc aatcctaaga aattataaag ctcgcccatt tccgtcgagt cacgggttgc
    12061 taaatattca ttgacggtaa ctacgtggac cccttcgcca gtcaacgcat tcagatacac
    12121 cggcatcgtt gcagtcaagg ttttaccttc accagtacgc atttcaggga tattcccatc
    12181 gtgaagcacg atcccaccca ttaattgtac gtggtatgga tataacccca atacacgttt
    12241 tgctgcttct cggacgactg caaaagcttc cggaagtaat tgatctaatg tttctccttt
    12301 ttggtatctg cccttgaatt cagcagtttt tgcctgtaat tgttcatcag ttaatgctgc
    12361 catttggtca gcgtatgctt caactttttt tgcaatgcta tctaaacgtt ttagttcttt
    12421 cttatcgttt tcgataagtg aacgaatcaa attggccatg cgttgtgtaa tctccttttc
    12481 cttctgtgta ttatccagac tatgtagact ttacttttca ttttatcatt agttatagat
    12541 aattgaaata tcttaagcgt attaaagtaa gaacttcata atttattcat atttcatcta
    12601 aaagaggagg aaactcaaaa aaacttgcgg cagctgtcag tagctgctgc aagttttcgt
    12661 tgttatttaa gtaaggctga tcagtctgtt tcaatcagtc cgtattttcc atctttacgg
    12721 cggtaaacga tacttgtacc atttgtttct gcatcttcaa aaataaagaa gttatgtccc
    12781 agcatgttca tttgtaagac tgcttcttca ctatccattg gtttcaatga aaggcgtttc
    12841 gtacgtacga tatctaattc agaatcattt tcgttttcgt cctcttcatt gaataaagca
    12901 gtagcatctg cagtattcat gcccgtttca cgggatttac gattaatttt tgttttgaat
    12961 ttgcggattt ggcgttccaa tttgtcaaca accaaatcga tacttgcgta taaatctgga
    13021 gaagtttctt cggcacgtaa tacaagataa ggtaatggga tcgttacttc cacttttgct
    13081 gttttttctg tataaacttt taagttcaca tgcgccgttg catcgggtgc gtcactgaaa
    13141 tagcgttcta gtttgcctac ttttttctca acgtagtcgc gaatcgcttc agttacttcg
    13201 atattttcgc cgcgtacatt atatctaaac ataaaaatta cccctttcgt ctaccacaat
    13261 caagaaggag aaggaccact ctttccctct tcttaattct attatatcga actctaggtt
    13321 atttgcaaat taaatcgcta tcaaatttcc acatctaatt catcttgcta aagaaatggt
    13381 tcggatgatt ttcggctgtg cgacctgcaa acaatctgct gcgtggaaaa gagtctgtcc
    13441 tgttgtatag acatcatcaa taatcaatat ttttttattt tgtatcttag aaaaggtgac
    13501 tgcaagttca aaaggttgag gcatctccaa tctttccttt cgcgtgtttt ttgcttgagc
    13561 cagcagatgc ttctctcttt tgagtagcat ctgaaaagga attcttgctg ctttcaacat
    13621 ctcgcttact tgattgaatc ctctttctgt tcgccttttt tcagataaag gtattggaca
    13681 atagatgtat gtcgggtatt ctttttttat ctgcgtccat ttatatgcaa aggtgcctgc
    13741 tagtcggata tctcccataa acttgtattg attgagccag gccttcatat tttcatcgta
    13801 cacaaaaaag gcacggtgct gaaaatcata ggtcggatat ttcttcttcc attcttgaca
    13861 gtcactgcag taaacgggaa tagactttac tttctttttc gtactctcta gattgaaatg
    13921 gattggtttt tgacagccag aacagcctct tgtttccaat aaatgaaatt gtgcgcgaca
    13981 aactgaacaa gtctgaggta atctgtattt cgaaacacca ataatctcgc tgaatgttaa
    14041 attttggacg atatctttac tgcagcaact gcacctcaca gcatcttctt cccttcttca
    14101 ttcatttctt tgatttcctg acaagctttt cgaatagctg cagttttttc ttcatagaaa
    14161 aagtatactt gactgttcgt ataaggccct ttacgatcgg ctcttcctgc gatttgtacc
    14221 aaagaagatt tagtaaaaac cggatgatcc gctcctaaaa cgataacgga tattctttca
    14281 aatgtgactc cacgttcaag gatcatcgtg gttaacagta tgtcatattt tgcttctcgc
    14341 attttctgga ctttttccgc ccgctgattg tctcttgaag aaacatctgt gatctcatat
    14401 ctcggaagag cctcttgaag tgtggctttc aaacgatgca tcatcgtgat acttggacaa
    14461 aataaaagga tatggttttc tttaattaat tgatgaatga tccgcaccaa agattttatc
    14521 ttcttcttgt tcttgcattt ttgtcgccaa ttattcatga aaaccaagac gggttcagga
    14581 agtaaacgcc gatgaaagcg caaagcaagt ttgtggatgt caaacgtctg cttgatttcc
    14641 tttagtaact ttttatcagg tgtggcactt aagtagatga atcgtccgtc tggtttaatc
    14701 gctgtttgca cagcatgagc taatcctgca tccccgttat aaggaaacgc atcgatctca
    14761 tcgatcacta ttaaatcaaa ggaccgataa aaacggtata gctgatgaat cgtacagata
    14821 acaaaagaag taaaggtata agccgttgct gtattcccat ggagcaacgt gattttttct
    14881 tctggaaaaa cataacgata gcgtaaaaac aattcattac aaacatcgac tcgcggtgta
    14941 caaatcgcta ctcttcctcc agcttttaaa gtttccacta aaataggata aagcatctct
    15001 gttttccctg cacctgttac agcccaaata agatgatgtc ttttttctga atggcaaagt
    15061 attctctttg cgatttccgt ttgtttttca gtaagctgtc ctgtccaaaa aaagacgact
    15121 tctcgaggtt ttgcttcagg ctgatcaaac aaatataaat cttgttgagt gtcgcatcgc
    15181 cctaaatata gacaatgtgt acaaaaataa ccgtaatctg caagctgtcc atcaatgacg
    15241 gacgtcctca ttccacagcg atgacaaaga atccagttgc ccaaacgttc cactgctgcg
    15301 atcttctcat aatttggtaa atttacctcc tcacagtcta ttgctttaat atctctcgtc
    15361 agaagccttc taccccaaaa atgattcata ttattcgccc taaaaaaata tacgaaaaaa
    15421 caagcgatct attttttgga tcgcttgttc gatagttata ctgttctatt tattcaccta
    15481 tgagcgtgat tgcttgatct aacacttttt cgccattcat cataccataa tcagccatat
    15541 tgatgacgtc taaaggaatg ccttttggtt ccagacgttt ttcaaaatca gctttcataa
    15601 agcgaacttg aggtcctaat aacagtacat ctacatcttt atttgcaaga ttgttatctg
    15661 cgtcagaagc ggacacagcg aaaatatccg agtcgattcc ttttgcttca gcagcttttt
    15721 gcatttttgt tactagtaaa cttgtgctca taccagccga acatacaagc atgattgttt
    15781 ttttagccat acttttctct cctttttaaa acaaactacg caaacgtttt cttcgcgtct
    15841 gttactctat tatataaaaa gaaagaaatt tgtcaacgtt ttctaaacgc ggtttattta
    15901 atcaaatagc caactagatt tcactaattt attgaacacg acatattctt tgtccaaatt
    15961 gatgacaact acttcatagc ctgcatttat ccagccatag gcaccttttt cagggtgttt
    16021 ataatcattt gcccaccaat ctgtactttc ccttgctgtt ttgggaagtc cgttactggg
    16081 aaacagcagt tcgtcaaatt gggtaaaggt caacgttact tgagagccgc cattgttctt
    16141 caaataatgc gtaattgcat cgtacttttt cattcgttcc tgcatcccca gatccaccta
    16201 cctttgtctt tattatcgca tactacagcg attgcgcaac cgttacagtg ttgaaacaac
    16261 ctagaacttt atttatactg aataaagaaa atgaaaaaaa gaggtgacaa gatgatcgag
    16321 cgctatcgta caataaaaga aaatggacaa agtgagattg agatcaaaaa atcacgcttt
    16381 atctgttctc ttagacgaat agaatcagag gaccaagcaa aaagtttcat tcaagaaata
    16441 aaaaaagaac attggaaagc gaaccataac tgcagcgcct tcatcattgg agaaaaaaat
    16501 gaaatccagc gaagcagcga tgatggagaa ccaagtggaa cggctggcgt accgatgttg
    16561 gaagtcctca aaaaaaatca actgatcaat gtagctgcag tagtcactcg atattttgga
    16621 ggaacgaaac tcggcacagg cggattaata cgagcataca ctcatgctgt ctctcaagca
    16681 ttgtctgaca ttggaatcgt agaaggtacc ttgcaacagg agatctctgt gatgatcacg
    16741 tatccgcaac taggaaaatt acagaattat cttgaactcc aacatttttt tataaaagaa
    16801 atccaatata ctgatcaagt tagcgtcatc tgtgctgtag atgaaaaaag tgctgaccac
    16861 tttaaagaat ccatcattga gctgctgaac ggacaagtcc aatttgaaga gggtcctgtt
    16921 tcttatcacg agcaactggt aactgatcat atataaaaac aggtgattct gattctcttt
    16981 tatttctaag taaggaatca gggatcacct gttttttatt tttccaaata aaactcaaaa
    17041 cgatttccta cgtattgcgt acgcacatac tcaaacggcg taccgtcttc aaaataagaa
    17101 atctggcgca aacgaagaac tgcatctcct cgttttacct ctaaatagtc agcaattttc
    17161 tcagaagcca acacagcagt aatcgtttga ttcgctggac cgatcttttt cccgcctttt
    17221 tcttctagcg tgcgatacaa cgattcagta atctctgctt tgctgtagcc atcgatcagc
    17281 ttttgtggca cactggcaac ttcaaaacaa atagggacat catctgcgaa acgaatacgt
    17341 tccattcgca agatcgtgtc atctttactc aagtttagtt tttccatttc acttgagctt
    17401 ggcgaagtca agaaataaga gattgtccga ctagacggta ctcgattttg cgattccata
    17461 atttctgtaa aacttgttgc accagacatt ttttcttgta ctttttctcg cgcaacatat
    17521 gtacccgaac cgatctttct ttctaagatt ccttcatctg ccaagttttg aattgcttgt
    17581 ctcaacgtca tacggctgac attgaatttc actgctaatt ctctttctga aggaagacgg
    17641 tctccgactt tccatatacc ttgttcgatt tcatttttta attggtcatg aatctgtata
    17701 tatacaggta attgattcga cataacgtac acccgtcctt tcgtttcttc tattataact
    17761 ggtatatacc tgattcacaa ctgaaaaaga taaaggtttc tatccatcaa cagaggattt
    17821 agacacacat cgttcttcct actcctattg tcagaaacaa ttttttagat atgttcaacc
    17881 tagaaaataa caaaagaccc taaaatcgat gaaaatgccg acttatggat cttttgttat
    17941 tattagcggt ttttagccat ccattttttt gtatgattcg ttgggcgttt tgattttacc
    18001 tggaccgctt ttccgccttt tttcggaata ttcacttcaa tattgtattt ttctgatttg
    18061 accaaatcat ataacttttc agctgcttcg tctacttttt ctttccattt catgtggatc
    18121 cctccaattc ttgtagttaa cttaactcta tcttaaatca gcagaatctt caaggaacaa
    18181 ggtagcaaag caaaaaaaag agctctcgaa ctaacgagag cttacgattg tggta
//
LOCUS       DBNIBO010000042        18160 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.045, whole genome shotgun sequence.
ACCESSION   DBNIBO010000042 DBNIBO010000000
VERSION     DBNIBO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.045"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(194..991)
                     /locus_tag="AC1Y77_001237"
     CDS             complement(194..991)
                     /locus_tag="AC1Y77_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIQ1574827.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(988..2172)
                     /locus_tag="AC1Y77_001238"
     CDS             complement(988..2172)
                     /locus_tag="AC1Y77_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIQ1574828.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2197..3444)
                     /locus_tag="AC1Y77_001239"
     CDS             complement(2197..3444)
                     /locus_tag="AC1Y77_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIQ1574829.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3659..5404
                     /gene="dnaX"
                     /locus_tag="AC1Y77_001240"
     CDS             3659..5404
                     /gene="dnaX"
                     /locus_tag="AC1Y77_001240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIQ1574830.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5424..5738
                     /locus_tag="AC1Y77_001241"
     CDS             5424..5738
                     /locus_tag="AC1Y77_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIQ1574831.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5763..6359
                     /gene="recR"
                     /locus_tag="AC1Y77_001242"
     CDS             5763..6359
                     /gene="recR"
                     /locus_tag="AC1Y77_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIQ1574832.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6423..7067
                     /gene="tmk"
                     /locus_tag="AC1Y77_001243"
     CDS             6423..7067
                     /gene="tmk"
                     /locus_tag="AC1Y77_001243"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIQ1574833.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7082..7411
                     /locus_tag="AC1Y77_001244"
     CDS             7082..7411
                     /locus_tag="AC1Y77_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIQ1574834.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7425..8363
                     /gene="holB"
                     /locus_tag="AC1Y77_001245"
     CDS             7425..8363
                     /gene="holB"
                     /locus_tag="AC1Y77_001245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIQ1574835.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8399..9223
                     /locus_tag="AC1Y77_001246"
     CDS             8399..9223
                     /locus_tag="AC1Y77_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIQ1574836.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9216..9563
                     /locus_tag="AC1Y77_001247"
     CDS             9216..9563
                     /locus_tag="AC1Y77_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HIQ1574837.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9632..10504
                     /gene="rsmI"
                     /locus_tag="AC1Y77_001248"
     CDS             9632..10504
                     /gene="rsmI"
                     /locus_tag="AC1Y77_001248"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIQ1574838.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10613..11734
                     /gene="dinB"
                     /locus_tag="AC1Y77_001249"
     CDS             10613..11734
                     /gene="dinB"
                     /locus_tag="AC1Y77_001249"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIQ1574839.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="AC1Y77_001250"
     CDS             complement(11788..12390)
                     /gene="nrdG"
                     /locus_tag="AC1Y77_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIQ1574840.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="AC1Y77_001251"
     CDS             complement(12705..14894)
                     /gene="nrdD"
                     /locus_tag="AC1Y77_001251"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIQ1574841.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15220..15987
                     /locus_tag="AC1Y77_001252"
     CDS             15220..15987
                     /locus_tag="AC1Y77_001252"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574842.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            15977..18019
                     /locus_tag="AC1Y77_001253"
     CDS             15977..18019
                     /locus_tag="AC1Y77_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574843.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5666 a         3330 c         3771 g         5393 t
ORIGIN      
        1 caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt
       61 ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt
      121 tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt
      181 catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat
      241 gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg
      301 tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc
      361 ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa
      421 agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa
      481 ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc
      541 cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag
      601 catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat
      661 aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca
      721 atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc
      781 ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat
      841 tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct
      901 catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca
      961 aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat
     1021 cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag
     1081 gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga
     1141 gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt
     1201 tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt
     1261 taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata
     1321 ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc
     1381 tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa
     1441 ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt
     1501 cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg
     1561 catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt
     1621 atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc
     1681 gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa
     1741 gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt
     1801 cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc
     1861 tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc
     1921 aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa
     1981 atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg
     2041 cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg
     2101 aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt
     2161 aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa
     2221 cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt
     2281 gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc
     2341 gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc
     2401 cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg
     2461 gttattttga gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc
     2521 cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat
     2581 ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg
     2641 catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac
     2701 aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct
     2761 taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt
     2821 gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat
     2881 ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac
     2941 atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa
     3001 gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata
     3061 aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc
     3121 aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta
     3181 ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat
     3241 gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt
     3301 ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact
     3361 catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact
     3421 tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca
     3481 attattgttt atttattata ccataggaag atgtgaaact tctcggacta gagacggaat
     3541 tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt
     3601 tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat
     3661 ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca
     3721 aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta
     3781 cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc
     3841 aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc
     3901 aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt
     3961 cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa
     4021 agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa
     4081 aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa
     4141 aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc
     4201 agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc
     4261 tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct
     4321 tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac
     4381 aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt
     4441 accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt
     4501 tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct
     4561 agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc
     4621 agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt
     4681 taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc
     4741 tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca
     4801 acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga
     4861 gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg
     4921 tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca
     4981 agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct
     5041 taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga
     5101 gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt
     5161 aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc
     5221 agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc
     5281 ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga
     5341 aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga
     5401 ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga
     5461 aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag
     5521 agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca
     5581 tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg
     5641 atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa
     5701 aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga
     5761 atatgcaata tccagaacca attgcgaaac tgatcgacag ctacatgaaa cttccaggaa
     5821 tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag
     5881 taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct
     5941 gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa
     6001 gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat
     6061 atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag
     6121 aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga
     6181 ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga
     6241 tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata
     6301 tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa
     6361 tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca
     6421 atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct
     6481 tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag
     6541 aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg
     6601 atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag
     6661 tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg
     6721 acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt
     6781 tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt
     6841 cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt
     6901 cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga
     6961 atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa
     7021 gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt
     7081 tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt
     7141 gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg
     7201 aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat
     7261 caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc
     7321 gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt
     7381 cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa
     7441 acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc
     7501 gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta
     7561 tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat
     7621 gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg
     7681 aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg
     7741 gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg
     7801 cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag
     7861 aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt
     7921 ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag attccattgg
     7981 aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc
     8041 aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa
     8101 agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa
     8161 aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc
     8221 aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga
     8281 tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt
     8341 tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat
     8401 ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg
     8461 agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat
     8521 tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg
     8581 tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga
     8641 tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa
     8701 aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt tcacagcgga
     8761 cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc gtactcgtat
     8821 tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa ttggtccatg
     8881 tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat caatcaaaat
     8941 ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat gcggacgctt
     9001 gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag aactccctga
     9061 ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt tgaatctgct
     9121 tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat atgaatggga
     9181 tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca ttatacgacg
     9241 gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt gaaatgaagc
     9301 atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat caacgtctgc
     9361 gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat gtgaagcagg
     9421 aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc catgtctgct
     9481 acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt ctagaagtga
     9541 tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga gaaatactgc
     9601 tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt tcaagggaaa
     9661 tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag aagatatgag
     9721 ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa
     9781 tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt tacatgaaca
     9841 taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa aaacgattgc
     9901 tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc tagttttagc
     9961 ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg ggatgacggc
    10021 tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc ttccacgtaa
    10081 gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta cgctgatttt
    10141 ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa tttttggaaa
    10201 tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc ttagaggaag
    10261 tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat gttgtctgct
    10321 tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt tgggaacgtt
    10381 aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc
    10441 agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc atgaattaga
    10501 ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct actaactttt
    10561 caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga ggatgatgga
    10621 gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg atatggatgc
    10681 ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc cattagtaat
    10741 tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa attatgaagc
    10801 aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca
    10861 agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac aagtacggga
    10921 agtattctat cgttatacag atattgtgga accagttagt atcgatgaag cgtatcttga
    10981 tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa tgatccaagc
    11041 agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca ataagttttt
    11101 ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg cccctcaaga
    11161 cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag ggaagaaaac
    11221 tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg aatgcacaga
    11281 aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta aagtcagagg
    11341 aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa aagagcatac
    11401 ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc aactagctga
    11461 aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag ttttgaaggt
    11521 caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat atatctataa
    11581 aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg gagtagaaaa
    11641 aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt atgaaaacat
    11701 cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt tgaaaccgga
    11761 aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga tttgttcata
    11821 ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt ctaaagattt
    11881 ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta ggtcacgttt
    11941 gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt ctaatttatc
    12001 ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga tatcttttgt
    12061 cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg tattcaaaaa
    12121 aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat
    12181 ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg cagctttgtt
    12241 gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc ggacaccttc
    12301 accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt agtcttttgc
    12361 taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta aggttcaaag
    12421 atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac tgacctaaat
    12481 ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat acaaagccat
    12541 cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa acggtgttca
    12601 aaagcttgca tctgcagtaa taagcccaac taaacgaaaa atatgaggaa ctatttttgt
    12661 ttagctattc ttattacttg atgcaggaca gctttttcac aacctcattt catgtgtttc
    12721 acacgtgagg aaatttcttt atggcggcca tgtaccatcg gacgagcttg cggatttcct
    12781 aaatagccac aagtacgttt gactacatca catgtttttg gatcatgatt tccacattct
    12841 ggacattcga accctcgttc tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa
    12901 tgatcgattg gcgtattcgt accaagatac ccgattttgt catatgcaaa atcccataca
    12961 gcttctaaag ctttcggatt ttgttggagc atagggtatt cacagtagtg gatgaatcca
    13021 cctgaacaat attttggata atccttctca aattccaatt tttcaaatgg cgttgggttt
    13081 ttacgcacat catagtggaa actgtttgta taatattctt tatctgtgat attttcgatg
    13141 atgccgaatt tttctgtatc caaacggcag aaacggtcag ttagactttc acttggagtt
    13201 gaatagacac tgaaatgata accatattca tccccccatg catctgcatt agcttttaat
    13261 tctttgacga tatcaagtgt gaaagctttt gcttctgggt ttgtttccca ttcgccaccg
    13321 aagaaagctg aagcaacttc atacaatcca atgtagccta acgatacagt agctcgacgg
    13381 tttttgaata attcattgac agaatcggta cgtgaaagac gtttcccgaa agcaccatac
    13441 atatataaaa ttggcgcatt tgctggaatg gcttctttac aacgttctac acgatagaga
    13501 agcgcatctt tcatgatttc taagcggtca gacagtaact gccagaattt attctgatct
    13561 ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa attcattcgt
    13621 ccgacattta cttcttgacc attttcgtct ttccaccctt gcaagaatga acgacagccc
    13681 ataggtactt tgaaacttcc agttaattca acgattttat cgtaattcag aatatcagga
    13741 tacattcttt tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt aggatcttca
    13801 gggtttaagt tcacaccttt tttcaaagtg aagatcagtt tagggaagat agctgtccgc
    13861 ttttcactcc ctaatccatt gattcgaatc tgtaaaattg ctttttgaat ttctctttca
    13921 aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc atttgaagta
    13981 aataaggtat tgatttcata ttccaaactt tgcatagcat cgaagatatc ttttttggtt
    14041 tttgcttttg catattcttt ttggcgttct ggcgaatcga tccattcttc cgcgtctttt
    14101 agatgttttt tatagttcaa ttctgcaaaa ggagcaagca attcatctgt tcggtcggca
    14161 gaacagccac catattgact tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg
    14221 gcggtctgga tagattttgg cgattcgact tcggcattgc cgattttgaa cccattgttc
    14281 agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg atgataatcc
    14341 aaatcatggt aatggatatc gcctttttga tgcgcatttg ctatatgtgg tggcaacatt
    14401 ttcagtccga ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca
    14461 ttactgtctt tatttgcgtt ttcgttcacg acagtttggt ccttgttgat tagcttttca
    14521 atcgaaaagt tgatatctgt tgctttactg cgagcaaaat ctcgtttggt acgataattg
    14581 atgtactctt ctgctaattc atattcgttt ttttctagta atatatgttc cacgatgttt
    14641 tgaatctcat agattttgac attttcagca aaccggcttt tgatttcttg gacaaccaag
    14701 tctgcaatca tttgaatttt ttcataagta agtggtgata atgagccgtg aattttcttt
    14761 tctgctttga tcagtgcttc attgatcttt tgttcatcga aatcaacatg gcggccatcg
    14821 cgtttgatga ctttcatctg ggatgtctct ggctgctgag taacgacagt tttttcttca
    14881 taatttactt tcattatctt caactcctgc taaattgttc ttgtctatca taaaacaaaa
    14941 tcggggaagg agcaactaaa tatcggctat atatagtatt caatgtgctg tataattgga
    15001 tttctatact atatgtagtg tatattttat tgtgaaaaaa ggtgaaaact tgtagctggt
    15061 ggatttgttt tttgcatatt taattttgct gaataaaata attgaataaa attttttatt
    15121 cagtccaagt aaaagtgaca aaaacataag agcaaattat tcgtagatat gctactatgc
    15181 aaataaatag aaatgaaaaa tgaaagagga ggagaagaat tggaaaagat gatcgaagtg
    15241 aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg tcaaagcctt agataatatc
    15301 tcattcacag tagaagaagg cgagtttgtt gggattatgg gcccaagtgg tgcaggaaag
    15361 acaacgctga tgaatatctt gtccacgatc aatttgccta cgatgggcag tgtctcgatc
    15421 caaggaaaag aaatcacaaa aatgaaaaac agtgagctca gtgactttcg tcgaaaaaaa
    15481 ctaggattca tttttcaaga gttcaatttg atcgataccc taaatgcaaa agacaatatc
    15541 ctattgcccc tagcagttga acggatgact aaagaggaaa tggaaaagag agtaagacat
    15601 gtagctcaat tgttgaatat agaagaactg ctcaaacggt atcccgatga actttctgtc
    15661 ggacagagac aacgaattgc agctgctaga gcacttgttg ttcagccaca agtgattttt
    15721 gcagatgaac caactggctc tttggactcg aagtcggcta cagaattgtt gaactacttg
    15781 aagaccatga atcaaaaaga aaaagcaacg atactgttag tcacccatga tccatataca
    15841 gcaagctact gcgatagaat tttatttatt aaagatggtg tgattttttc tgaagttgtc
    15901 agaagaggga caagaagaga gttctttgag aaagtaatag acatgcaggc gacgattggc
    15961 ggaggaggaa aaatgaatgc tgtataaact agccgttaaa agtttttttc ggcagtcacg
    16021 aggctatctt gtttattttt tcagtttgac tttatccgta atgatctatt attccttcag
    16081 cgcaatgacc tacgatcaat ctttagtcag aagagcaagt caagatactt cgatagatgg
    16141 tggattgagt tttggtggat ttatcattac tgttgttttg ctttttttcg tattcagtgc
    16201 caaccgattt ttcttgaata gaaggcaaaa ggaaataggc atctatcagc tttttggtat
    16261 gaataaactg cagatatcag gtatatatgt gttagaaatc atgataattg gattgttcgc
    16321 ctgtatttct gggatattac tcggtattat tttttccaaa ctcttttcaa tgattttggt
    16381 tcgaatgatg gatatggatc ttactagtcc attttttatc tccattccat cagtagtaga
    16441 tactttattt gcttttttca tcatcctttt agcagtatct gtatattcaa tatggaaaat
    16501 atggcgctat ccaatgattc gaagttttgg agaaaaagaa caagcagata gttcaacaat
    16561 gcgcttcagg acaaggcacc gattactcgc agtcatcggg ttactacttt taacgagcgg
    16621 atattttgga gctagtcatt ttagggaaat cacttcatgg atgatcagtt atcattatct
    16681 tgatacatta gttttctttt ttcctttttt aatttttttc gtttgtgtgg tcggtactta
    16741 tttgtttttc tgttatacat ttcggttatt tttgatcata ttgagtaaat ggaagattta
    16801 ttactatggt atcaattttt tgataatggg taacacgcaa gttcatttgc tcaaaagttg
    16861 gcgtacaaat tctctgatta cagtcattct cggaatttct ttaatgacaa ttggcggcac
    16921 ggctagtatc tctactattt tatcctacca ggagaaagtt tcctccccga tggactatca
    16981 gtttgatgtt gagacagaga aaaaaatcaa gccgattcta gcagaggaag ggcagaagat
    17041 aactaaagaa atcaccttgc attataaagt cattgggagt atctatagtc aaaatctcga
    17101 agaacctatg tttcaaactg ctaatctgat cacggaaaca caatatcaag ccttcagaaa
    17161 agttaatcca gatttgccga acatttcttt gaaatcacct ggtcatactg ttttgcttga
    17221 tcaaatgcaa actctttttc ggaatatttc attatatgga tctagtattg gactgccaga
    17281 taatcaagaa ttgaagattc aacaaatgct tccaagtgta ttaggtgacg aaacaatggt
    17341 atatctaagt cctgtactga ttgtagatga taccgttttt gaaaaagcaa aaggtgtgga
    17401 ttatcaagta gtcaatgtag atgtatcagg tggaaataat gaaaaaatag atgaacgact
    17461 gagcaatgac ctgtcgataa agtggctcaa tgctgtttat tattcttatg atatcgtaaa
    17521 tggaagtcta caaggagaaa ttagcacaaa gaaacttccg gatgaccaaa tgaaggaagc
    17581 acaatttagc caagaaagct ctcgtttgaa tttctcttcc agctattcga aattgcgtgc
    17641 agttaatcgg cgaatcggta tcaatatttt tgtcacacta tttgtcggga tgatcgtgat
    17701 tattgctact ggtagtattt tgacagtccg gcaattagca gaagcagagg aagaaaaaga
    17761 aaattatcac ctattgacta agctggggat tccacaaaga aggatcagac ggatgatctt
    17821 cgagcaaaac gcacttacct tctttccacc catgatatta gggatcactc acgcagtatt
    17881 tgcaatcaat gtcttcactc agtatataaa aactgccgat tactggttgg cttatcttgt
    17941 gagtggctta ttgatcctta tctatctttt gctttatgtc attacgagtc agttatattg
    18001 tcggattatc gaggaatgat atacggaata agacaagata ataaatagaa ctaaattttg
    18061 aataccattt atttggaata agaggttgtg acacgatttt tgtcacaacc tctttatttt
    18121 ttatattttt taaaagtacg agaaattata aagaaaaaag
//
LOCUS       DBNIBO010000043        17959 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.046, whole genome shotgun sequence.
ACCESSION   DBNIBO010000043 DBNIBO010000000
VERSION     DBNIBO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.046"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="AC1Y77_001254"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="AC1Y77_001254"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIQ1574844.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="AC1Y77_001255"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="AC1Y77_001255"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIQ1574845.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="AC1Y77_001256"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="AC1Y77_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIQ1574846.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="AC1Y77_001257"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="AC1Y77_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIQ1574847.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="AC1Y77_001258"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="AC1Y77_001258"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIQ1574848.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="AC1Y77_001259"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="AC1Y77_001259"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIQ1574849.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="AC1Y77_001260"
     CDS             complement(8054..8524)
                     /locus_tag="AC1Y77_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HIQ1574850.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="AC1Y77_001261"
     CDS             complement(8570..8995)
                     /locus_tag="AC1Y77_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIQ1574851.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="AC1Y77_001262"
     CDS             complement(9062..9331)
                     /locus_tag="AC1Y77_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIQ1574852.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="AC1Y77_001263"
     CDS             complement(9342..10937)
                     /locus_tag="AC1Y77_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIQ1574853.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="AC1Y77_001264"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="AC1Y77_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIQ1574854.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="AC1Y77_001265"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="AC1Y77_001265"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIQ1574855.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="AC1Y77_001266"
     CDS             15403..16377
                     /locus_tag="AC1Y77_001266"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIQ1574856.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="AC1Y77_001267"
     CDS             16547..17947
                     /locus_tag="AC1Y77_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIQ1574857.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4878 a         3971 c         3240 g         5870 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatat
//
LOCUS       DBNIBO010000044        17715 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.047, whole genome shotgun sequence.
ACCESSION   DBNIBO010000044 DBNIBO010000000
VERSION     DBNIBO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.047"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            4..816
                     /locus_tag="AC1Y77_001268"
     CDS             4..816
                     /locus_tag="AC1Y77_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIQ1574858.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="AC1Y77_001269"
     CDS             813..1613
                     /locus_tag="AC1Y77_001269"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIQ1574859.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="AC1Y77_001270"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="AC1Y77_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIQ1574860.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="AC1Y77_001271"
     CDS             3110..4819
                     /locus_tag="AC1Y77_001271"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIQ1574861.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="AC1Y77_001272"
     CDS             5031..9383
                     /locus_tag="AC1Y77_001272"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIQ1574862.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="AC1Y77_001273"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="AC1Y77_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIQ1574863.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="AC1Y77_001274"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="AC1Y77_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIQ1574864.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /gene="rnpM"
                     /locus_tag="AC1Y77_001275"
                     /gene_synonym="ylxR"
     CDS             11221..11514
                     /gene="rnpM"
                     /locus_tag="AC1Y77_001275"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIQ1574865.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="AC1Y77_001276"
     CDS             11511..11822
                     /locus_tag="AC1Y77_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HIQ1574866.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="AC1Y77_001277"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="AC1Y77_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIQ1574867.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="AC1Y77_001278"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="AC1Y77_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIQ1574868.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..15127)
                     /locus_tag="AC1Y77_001279"
                     /pseudo
     CDS             complement(14623..15127)
                     /locus_tag="AC1Y77_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002828906.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15292..17655
                     /locus_tag="AC1Y77_001280"
     CDS             15292..17655
                     /locus_tag="AC1Y77_001280"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HIQ1574869.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6037 a         3146 c         3902 g         4630 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagttat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaag
//
LOCUS       DBNIBO010000045        17665 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.048, whole genome shotgun sequence.
ACCESSION   DBNIBO010000045 DBNIBO010000000
VERSION     DBNIBO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.048"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            111..1076
                     /locus_tag="AC1Y77_001281"
     CDS             111..1076
                     /locus_tag="AC1Y77_001281"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIQ1574870.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1175..2347)
                     /locus_tag="AC1Y77_001282"
     CDS             complement(1175..2347)
                     /locus_tag="AC1Y77_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574871.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2334..3479)
                     /locus_tag="AC1Y77_001283"
     CDS             complement(2334..3479)
                     /locus_tag="AC1Y77_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574872.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3493..4425)
                     /locus_tag="AC1Y77_001284"
     CDS             complement(3493..4425)
                     /locus_tag="AC1Y77_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574873.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4512..5621
                     /locus_tag="AC1Y77_001285"
     CDS             4512..5621
                     /locus_tag="AC1Y77_001285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1574874.1"
                     /translation="MTFVTMKESAMHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYL
                     LIAVLFTGVSYLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFL
                     LGFLLISTVGNHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQEL
                     SEQNKALALSQETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLK
                     EPLENLSATVHTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNE
                     TQQNCFLMVIKEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGL
                     KTMRQRVQEQKGQVHIFQENQQFQLTIILPREGHQ"
     gene            5618..6247
                     /locus_tag="AC1Y77_001286"
     CDS             5618..6247
                     /locus_tag="AC1Y77_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1574875.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6357..6584)
                     /locus_tag="AC1Y77_001287"
     CDS             complement(6357..6584)
                     /locus_tag="AC1Y77_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1574876.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6687..9275)
                     /gene="recD2"
                     /locus_tag="AC1Y77_001288"
     CDS             complement(6687..9275)
                     /gene="recD2"
                     /locus_tag="AC1Y77_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIQ1574877.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9295..9954)
                     /locus_tag="AC1Y77_001289"
     CDS             complement(9295..9954)
                     /locus_tag="AC1Y77_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1574878.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10003..10506)
                     /gene="trmL"
                     /locus_tag="AC1Y77_001290"
     CDS             complement(10003..10506)
                     /gene="trmL"
                     /locus_tag="AC1Y77_001290"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIQ1574879.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10727..11593)
                     /locus_tag="AC1Y77_001291"
     CDS             complement(10727..11593)
                     /locus_tag="AC1Y77_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIQ1574880.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11702..12343
                     /locus_tag="AC1Y77_001292"
     CDS             11702..12343
                     /locus_tag="AC1Y77_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIQ1574881.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12405..13766)
                     /gene="mgtE"
                     /locus_tag="AC1Y77_001293"
     CDS             complement(12405..13766)
                     /gene="mgtE"
                     /locus_tag="AC1Y77_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIQ1574882.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13784..14683)
                     /locus_tag="AC1Y77_001294"
     CDS             complement(13784..14683)
                     /locus_tag="AC1Y77_001294"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIQ1574883.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14685..15482)
                     /locus_tag="AC1Y77_001295"
     CDS             complement(14685..15482)
                     /locus_tag="AC1Y77_001295"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIQ1574884.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15460..16164)
                     /locus_tag="AC1Y77_001296"
     CDS             complement(15460..16164)
                     /locus_tag="AC1Y77_001296"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIQ1574885.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16295..16870
                     /locus_tag="AC1Y77_001297"
     CDS             16295..16870
                     /locus_tag="AC1Y77_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIQ1574886.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16988..17665
                     /locus_tag="AC1Y77_001298"
     CDS             16988..17665
                     /locus_tag="AC1Y77_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HIQ1574887.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5232 a         3794 c         3142 g         5497 t
ORIGIN      
        1 aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt ggttaaccag
       61 caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc atgaaattcc
      121 attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa gtagcagaaa
      181 gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat agtgaataca
      241 aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt ccttggatag
      301 aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga ggtgacggca
      361 ccttacatca ggtacttgat actttctatc agatggaagt agaattccca gttgcttata
      421 ttccagcagg ttctggaaat gattttgctc gaggggcagg ccttccaaaa aatcccaaaa
      481 aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata atggcttatg
      541 aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata ggattagatg
      601 cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac aaatacaact
      661 taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa aaaggatttc
      721 caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt ttatgtacag
      781 caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta
      841 aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca
      901 ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac tacacaacaa
      961 acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga gaagaaatgg
     1021 aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg tgttaataaa
     1081 agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag gactgtgaca
     1141 cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa ttgggttgcc
     1201 atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc gcagcaaaag
     1261 caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg taactgagtg
     1321 tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat gccgctattt
     1381 tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata aagcaactgc
     1441 ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc aatgaagtga
     1501 tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa atatgatacc
     1561 ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt aagaagccac
     1621 tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc aagtttcgtt
     1681 tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaac gctagattga
     1741 tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa ttaaaggttg
     1801 tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca acttctccag
     1861 taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta tcttcttctt
     1921 gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa
     1981 aggcatctgg gcgactctgt atcttgatcg tcgccatctt tcctacttcg acttgttcgg
     2041 taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga gcatcatcga
     2101 tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc aaatatttac
     2161 gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc gcatcttgca
     2221 gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca acagtgataa
     2281 ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc gttttataaa
     2341 gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag gaaaataaat
     2401 ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa ataaagttta
     2461 tacaaacttt cgctgatcaa gttgacgaga tttaattgac caacaatcgg aatatgaaga
     2521 tcaatccaat acttcacttg ttccgacccc atcataccag caaaaaagct catcgcaatc
     2581 gtcactgaaa tcaagatgct atccttttgc accagattca tttttggtat caaattacca
     2641 accaatgtac ctaacaaaat cgcacacaac gaccccagag cggtaacaat catcaaccat
     2701 tgccaacgtt caccaaagtc tacttgatag ataaaacgaa agacagctaa cataatataa
     2761 atctctgtca aaacaatcgt aaagcttgct gccaaattag cgattgaaac taacgattgc
     2821 tttctaggaa tcaaggacaa gcgaatacca tttgacgatt gatttgcttg ctgatcctgt
     2881 acattgcgga ccccccacat cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa
     2941 taaaaacttt tgaaactcat attacgacct gcatgaactt cttgaaaaga tacttcttgt
     3001 tcaaaaacag tcatgatggg cgcgagatca acgttctcca cttctgccac tttactcgaa
     3061 attataccaa tacgctgtaa aaattgatcc atgacgtttt tcatcaacgt ctgactcatt
     3121 cctgcttgac ccacatggag cgaaacagat gaaccatcaa tgagataaaa accagccaaa
     3181 tcatctgcat ttagcttttc attcgcttct acttccgata gttcctgtac ttcaaataat
     3241 ggtttatcat tgacttcgac ttgttctaag actgtggtaa aattttccga aagcgctgtt
     3301 tgatccgctt tgattacaat tccagtatta atcgtttcaa gattgccacc ttgatcaatc
     3361 tcgccaaagg ccgccataaa catcaatcct aaaagaattg gaaaggccaa tgtccaaaac
     3421 aacatggatt tatttcgcag cagtattttt gtgcgatatt tataaaaatg ccaaaacatt
     3481 atttcccctc ctctagtctc tcaaatcctt gccagtaatc tctaaaaaga catcattcaa
     3541 ggacgcttct ttggttgcta tttttccgta actgatcttt tcttcttgta agattgtaag
     3601 aatcggcaac aatactgtat ttagttcagt agttgttact tggagctgac cttttttata
     3661 agcgattcca tcagaagcta ttgttcgctg aatcgctgac aacacatttt ctgggagtaa
     3721 tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag attctttggt
     3781 tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa gaatttctac
     3841 ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca gctccttgat
     3901 actttctaaa attttattcc gactttgggg gtctacagct actgtcggct catctaaaaa
     3961 aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt tcaagccacc
     4021 actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt gaatcacctc
     4081 ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa aataatcgat
     4141 attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat
     4201 tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa tggttccttt
     4261 gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac tgccgtttgg
     4321 acctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat tcaatgctac
     4381 aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga aaaactcctt
     4441 ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag tgacgattgg
     4501 ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa tctcggctac
     4561 tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt caaggattcg
     4621 ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc agttatttac
     4681 tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt gcctttttac
     4741 ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc
     4801 ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat catcccccca
     4861 tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga aaaaaagatc
     4921 aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat tgggaaaaac
     4981 aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca caattagcct
     5041 tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat gtcggtcatc
     5101 tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac
     5161 taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat cggattcgtc
     5221 aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta gagtttctga
     5281 tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga ttgaatgaaa
     5341 cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt atcaaacata
     5401 gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat cgcttgcaaa
     5461 tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt ttgaaaacga
     5521 tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa aaccagcaat
     5581 ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag tcattattga
     5641 tgatgatcca tttgtgacga aatcgttaca aaccatttta gaaagcacga aagagatccg
     5701 tgtgttaggc attggtcatt gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc
     5761 cgatgttttg ctgacggaca tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga
     5821 aattttggct aaatttccca aagccatcct tttgcttttg acgacattca aagatgaaga
     5881 atacatccgc gaagcatttt ccatcggtgt caaagggtat ttaatcaaac agaacttgca
     5941 agcgattatt ccttctgtca aatccgctta taatggacag gccgtatttg gtaatgaaat
     6001 catcgagact ttcactcaaa tgatgaagaa taaaccgacg attgatagtc atgcttatgc
     6061 ttcttttagt gaacgtgagc ttgcaatcat caaagaagta gctgccggga aaaacaataa
     6121 agaaattgca gatgccttgt acttgagtga cggcactgtt cgcaattaca tcagccaatt
     6181 actagaaaag ctagatttac gggatcgcac gcaattggct atctattttt atcagcattt
     6241 gcaataagtc ttatctaaca caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt
     6301 tttttcacat cttctattca attgtcccgc aaaccatttc ctcacgattg aaagaatcaa
     6361 ggattcgcag ggctgccagg attcgttgga ttgttcggcg tgttaggcgg aaaaataggc
     6421 gccttgtcta acgttttttc tagttttgat tcttcacgat cgtctcgttc tacatcagga
     6481 ctttcattca atttgaattc tttttccatc tcatctaact ttttaaagtc attgccagat
     6541 tcttttgcta atttcgctaa tttatcttcg gtttctttag ccataaagta acgcccccct
     6601 tttgatttca gcttagcata tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca
     6661 tttttagcta tttctcatct ttaaaatcaa gcattttttg ccggtataaa atcgtaggga
     6721 gaactgtttt ccataccaat catcggatct actaactggt tatcgatcac ttctgctgta
     6781 agaacgaaat ctttagaaac agcaggttct acctcttctt tcttttcttc tccaacaaat
     6841 gaagaaaaca gatctggttc cactgtttcc attacttggt ttttcgtttc attcgtccca
     6901 ctgtcatctt ctacaggtag cttttctttt acaacccgtg gctcttttgt atagttccca
     6961 tcttcaaatg gatcatttcc gaccaaagaa tcttcataag ctgctagttt ggtccggaga
     7021 gtgatattca ttttatcatt atccatgatc cttgatttca acgtagtcat ccggcttgct
     7081 gattcattcg ctacacaagt gacataggct tgctcttccc ctaacaaaat caacagttcc
     7141 ttactgcgag tcactgccgt gtagagcaaa ttacgctgaa gcatccgctg atattgatgg
     7201 accatgggca aaataaccat cttgaattca ctgccttgtg atttatggat tgagcaacaa
     7261 taagaaagag tgattttatt ccattcgttt cttttataag tgacctcatt tgcatcaaac
     7321 tggataacta attcatccac tttatcttca gaatcttttg ccaagatgat acccgtgatt
     7381 tctcccatat ctccattgaa tacattcaat tcgggagtat tgactaattg gaggactttg
     7441 tctcctatcc gataaacggt gtcgttccaa tgtacttcct tttttccagg tttccctgga
     7501 ttgaatatct cctgcatcat tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt
     7561 ggtgctaata cttggatatc ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt
     7621 ttagagatca agggttcaat ttgataagca tcacttcgga aataggaacg atctttttga
     7681 ttctttgtga aatcttttgg cagacgtcct tctttgatct catgggcaag aggaataata
     7741 ctagaaccat caccttgccg ataaatctga ttgagttcca ttttgggaat ttgttcgatt
     7801 tccaaaagat catggagtac ttgaccagga ccgacagagg gaagctgatc tttgtctcct
     7861 actaagataa cttgcatatt cgtcggaatc gctttgaata atgtgttagc cagccatgta
     7921 tcgaccatag acatctcatc aacaatcaaa agtccgcctt ctaattcttt cgtactgact
     7981 gaaggtgttt tttcccgtcc attcagcccc agaagacgat ggatcgtact ggcaggcaga
     8041 cctgtcgttt cgttcattcg tttagctgct cggccagttg gtgctgcaag tagaatagga
     8101 aaaatttctt gggtatattt tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga
     8161 ttgacgattc cgttgatcac agtagttttc cccgtacctg gtcctcctgt taaaataaat
     8221 agaggggaac ggattgcttc agcgatagct tgttcttgcg aaggtccata ctgtatgccg
     8281 aatatttttt cgatacgccg aatattcttc tgcacttctt tttcaggata atggatttct
     8341 tttttacgac tgagaaggcg ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa
     8401 ctgttttcgt aaagattagt ctcttcttgt tgtatctttc cttcttcaac aagttggata
     8461 attacagtag caacctgatc aggctggatt tcaattgggc gacttgtttc taaaatacgg
     8521 atcgtttgct gaagcaatgt ctccgccggt acgtatgtat ctcctgtttg aactgcatgc
     8581 tgaaaaatct gatgcaagat agctgctcga attcttctcg gtgaatctgc tgcaataccg
     8641 atttgttcgg ctagattatc tgcacgtttg aacccgattc cttctatgtc ttcgactagc
     8701 tggtacggat tttcttcgat gatatccaaa gactcgttct tataggcctg gtagatactg
     8761 aaagctaact gactaccgaa cccgtaacga ttcagtccca tgatcacttg atccatcccg
     8821 tggtttagcc gaatcgtctc agcaagcatt tctctttttg gcttcgtcaa accggagatt
     8881 tgttttaata ccgaaggatc agcaatgatt ttttcgatag catcttctcc cagcaattct
     8941 acaatttttt ctgctgtttt cttcccgatt cctgggaatt tttcgctgga aagatacgct
     9001 acgatcccat ttgcagttgt tggcttttct tgctcatatc tttcgagttt aagctgcctt
     9061 ccgtatttag gatgatccac taattctcca taaaaacgat aagtctcatt ttcttgaata
     9121 tcgccgaaac ttccggtgac tacaatctct ttttcacggt aatcagctgt ggtttcactg
     9181 atgtgtacca aaagcacttt gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact
     9241 gtaccaacga aataaggcat ttcttctggc tgcatcggaa gccgcctcct tcttctatag
     9301 taaaggttct ggtgctgttt cagttcctaa aaaatctgtt tggttccata ctttcagttc
     9361 atatggcaat aatctgcaag gctcttcagt ctctaaaatc gtcaaactac tattgaacaa
     9421 tccacccatt tcacgcaact cacttagctc ttttcctgtc atccattgaa tggctgcagt
     9481 taaagaagct ccatgtccta cgaataaaag tgggccatct ttatgtcggg caaccgcatc
     9541 tgctacaaca gtttcgatac gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga
     9601 aggatcatac agatctaaat gattacgcaa attcggcaga ttatccggat atttctggta
     9661 catttcatcg atcgactgac cttctaaagt acccagccct agttctctta gcttatctgt
     9721 ataaaaaatc tctaccggac gagcaagctc tttgttgata ccttctgctg tatcacgtgc
     9781 tcgctttgct gtactggaat aaattgcttt aaaaggaaca tattttacct ttttcccaaa
     9841 tgctttgatt tcttcgtaac tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa
     9901 acgtctttcc tgattccatt gtgtcttccc atgtcgggta aaatatagtt ccatacgcag
     9961 caaactcctg tctttctttt attttactct ataataaact gtctaatcca gcttgtcatt
    10021 ttcatagtga tgcgtcaatt ctaaattagg gaatccttgc tggcgaagtg cttcatacac
    10081 tatcattgca gctgtattcg ataaattcaa tgagcggaca tgctcatcat tcatagggat
    10141 gcgtaaacac ttttcttcgt tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt
    10201 tccaaataaa aagaagtgct cttctccatc agcaaaattt tcgtcactat atactttatg
    10261 ggcaaatttt gagatcaaat gtagtttacg attacctaaa aatgttaaaa aagcttctaa
    10321 attactgtgg taagtaatat ttacatcatt ccaataatcc aaccctgccc gttttagatg
    10381 tttgtcatca gttgaaaaac ctagtggctc gattaaatga agcggagtat tcgtggctgc
    10441 acacgtacgg gcaatatttc cagtgtttgc agggattaat ggttcaaata aaacgatatg
    10501 attcatcagg atcattcctc tcatatttat gttcgtttct attttctgac aatttgattt
    10561 ttgcataaaa aaaaacgtat caactcggtg tcagtcactg cgagtcgtta cgttagtgaa
    10621 gctcttctca ctaactttta tcagaacagg aacctgataa aaaccaatga aaaaacaact
    10681 tgttgctaat ataacaccct caactttttt ttgcaacctc tactcattaa aaactgctag
    10741 aaaaacgttt tctcttagtt ccttttaata attctatgtc aattgtaatc ttcttcaaag
    10801 gattcttata taagtctgct ttgattcgct cttctacctg ccattctaaa ggtgacatgg
    10861 ccaaaagatc tttttgattt tcctctggta tctcaaattc ataggttaca cgctgacgtt
    10921 cagttaattt cattgcttca gcaaacttgg ctactacacg ttcattggaa taactctgtt
    10981 tttcaggttg attcggataa aaagcctgac gtagttcctt taaataattt tcttgtggca
    11041 ccaccttcaa cacttctcca tcttctgaga gtacacgttg gaattcttgg taatgagaag
    11101 gcgaaaagat atttaaaacc gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat
    11161 cagctacaca ccaaaatgct tcggcagatt gttctgtagc tgcgtagact ccttcttttg
    11221 aaatatcaaa tccaacagca ttttgaatct ttttcaattt tgaaagttca ttcaagaagc
    11281 taccttctcc gcagccaaca tctaatagcg agacttcttc aggtaaatgc tgcgcaatag
    11341 tctcaataac gggctgatac atgccagatt ggatcattct acgccttgga acgaacattc
    11401 cttgatcgta ttctgtttta accgcatggg ataaaaaata aagtgttcct tttttggaaa
    11461 ggtcaaaacg gtgattattt ttgcatatca agccattatc agactttttt acttcttctt
    11521 ggcaaaccgg acagcggaac agctgatgat tttctgccaa aaactggcgt cctcggtcaa
    11581 ttttttttaa catagttccc cctcatttct gttttatctt accgttagat gctcactttg
    11641 gcaactgcta aaaatatgat acaatgccta gaagaaagtg aaagataaag gagaagatta
    11701 gatgatcaaa gaattttgcg ctgaaaacta cacatctatt cctctagcaa ttgctagagg
    11761 agccgatcgg atcgaattgt gtgacaactt agcggtcggt ggcacaaccc cgagtactgg
    11821 tgtgatcgaa gaaacgttaa gttatgctgg ggaaaaagat atccccgtaa tgacgattat
    11881 tcgtcctaga gggggcgact tcgtctataa cgatatcgaa ttgaaaatca tgcatactga
    11941 tttgattgaa gcaaagaaat tagggacgga tggcgtagta tttggttgtc ttactccttc
    12001 aggctggcta gacgaagagg ccttagaact attgatcgaa acagcagaag ggttgcagat
    12061 caccttccat atggcttttg acagtattcc tgaagatcgg caattcgaag caatcgattg
    12121 gctggtagaa catggtgttg accgtatctt gactcatggc ggtcctgctg acaaaactat
    12181 tgtagaaaat ttcccacggc ttaaagaatt gattgcctat acagatggcc gcttaatcat
    12241 tttgcctggt ggtggtgtca ctgcagagaa tgcagcacat gtagctaaag aattgaacgt
    12301 aagcgaagta cacggaacca aaatcgtgga tcttcagtta taatcttata aacaaaaaaa
    12361 catttgcaac aggcataatg cttattgcaa atgtttttgt ttttttacat aaaatagccc
    12421 ataaataaag aagcaatatt gaaataaatc agtacactcg tcaaatcgct caaagtcgtg
    12481 ataaagggac cgcttgctac tgctggatca aatcctagac gatccattag catcgggatt
    12541 aaactacctg caagattcgc cactgtgatt gcaaatagca tagccatccc tatgacaaac
    12601 cccaagataa aattatgttt ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca
    12661 gctcctgtga caacgccggt caagacttca gccaaaacaa gtcgaacaaa tccatgatct
    12721 ttctcatcag aaatcgcaag tcgacgaaca gccacagcta gcgactgggt tccagcatta
    12781 ccggcagttc ctgtaatcag cgaaatgaag actgctaaaa tactcgcttc acttaccaag
    12841 tcttcatagt gactgattaa agaagcggtc gacataccta aaaacaacag agtgatcagc
    12901 caaggcaaac gtcgagacgc tgcctttact ggattttcat taatttcttc cacattgacc
    12961 ccggcaagtc ctgaataatc gctagcagct tcatcatcaa taacgtcgat gatatcatcg
    13021 actgtgacga ttcctaacaa atgatcatca taatcggtaa caggaagagc taagaagtca
    13081 taatctctaa atgtttgtgc cacatcttct tggtcgtcgc ctacatgaac tgaaagcact
    13141 cgttcgctca tcagatcaga aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga
    13201 gaaataaccc ctactagctg accttcttga ttaacgacat agatgtaata gattgtttca
    13261 gctacatctg cttcgttctt cagcacgtac atcgcagagc gaacagtttg attggctacg
    13321 atcgaaacaa attcagttgt catgatcgcg ccagccgttt catcctcgta atggagaagt
    13381 tccttgattt cactggcatc gtctgccgac atcaaactta aatatttagc aatttgcttt
    13441 ttatccagcg tattaagcaa gtcaacagca ttatctgtat acatctcaga aagcatatca
    13501 gctgcatagc taggacgcat ttcatctagg tattctttca tatgttcgtt gtcttcttca
    13561 atcacatcga acatatccgc taattcttta ggcgacagat aggaatagat cagctgcctg
    13621 ttctcttctg taagagattg gtagaattgc ccctgctcat agatatgcat ctctaaaaaa
    13681 ctgtttctga atgcctgaat attattttcc cttaaatctt gagtcaactg ggcaaatctt
    13741 tcctctaatt cttgattttc atccaaacca atctcacctc gccttattct tcaaaccgtc
    13801 tcacaactgc ctccatgtct gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa
    13861 atggatggta aaaacgcaga tcataacaat gaagggcttg tctttttatt cccagatcca
    13921 ttcgcccgcc atacatatca tcaccgagca gggcacagcc aattgcggaa aaatgaactc
    13981 ttatttgatg tgtccttcct gtatgcagct ggatatcaac taacgcgatt tctgttagtc
    14041 gcttctctat ccagtactca gtctgtgccg aacgtccttc atcagagacc attcgcttca
    14101 atagcgaaga ataatctcgg gcgatcggca aatcgatatg tccatgtgcc gataaatctg
    14161 aaagccttcc actgactaag gcctgatatt ttttcacaaa cttcttttca cgaagttgca
    14221 tatctaattt tgcgtgcgca aatccatgct ttgcaaaaag catcaagcca gatgtatccc
    14281 gatctagtcg cgtcaccaca tggattactt ggttttcgta accttgtttt ttataataag
    14341 cctttacacg attagccatt gtcccatttg gatggtattg ggcaggaata gacgaaacgc
    14401 ctgctggttt atttactacc agaacgtgtt catcttcata cacgatatcg atcggtgttt
    14461 catccaaaag tacggtttca tgttctcctt cagcaggaat cacaatggtt accttatcat
    14521 ccttagctag agaaaacaag acattttcta cttgatcatt aactttgatt tgaccgcctt
    14581 gaaattttat tttcgctaac agaccctttg agatcccttt ttcttttaaa aaatatttta
    14641 cttgctgaga atgatctttc tcataatccc atgtaaactg catatcaatc ctcactaata
    14701 aaggcatctt tcacacgatg ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt
    14761 tcttctgcta ttcgataata aattgagcgg atatttgctt ttgcgacatt caattgatca
    14821 accgttacaa gataatcttc tgtattttcc aattttattt ctagccattc atcatgggca
    14881 atcacgatcg gagaacccaa agtacgaaac actcgattat tcagtgaagc aatctctgcc
    14941 aactgaaagg cattgatact aggatggatc acagcaccgc cgacactttt attatatgct
    15001 gtcgaaccag taggcgtaga aatcgtcaat ccatctcctc taaaactttc aaacagctcg
    15061 tttttgatgt atacatcggc taccatcgta cgattggccc gtttaatcgt tgattcattc
    15121 agtgctaaaa aatgctggtc cggtgtttca tccaaataac tgatccgaac atccagcaag
    15181 ggataactaa cactttgttc ccggttcgtt cgtaagctgt ctactagttc ttccagctcg
    15241 taatcccgcc aatccgtgta aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca
    15301 tcattcagac aatgactgta gtgatggaac gccgacaata atgtgccgtc tccgccgact
    15361 gagatgacca attctggctc atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg
    15421 ttcaaacgtt ctgtcacttc tatagtcttt gcttctttat tatgaacgat cgctactttc
    15481 atcccctgcc tcctctcttt cgctttcttt cttcaaaagc gttcgatagt agacatcatc
    15541 tgattttcct cttccatggg agaataaccg ctgtgcttct tggatttctt cacggatact
    15601 ggacatctct tcatccaact gataggcagc ttctgccgca cgctgaagtc gttcactcat
    15661 ttcttctgga aaaacgcctt gatatttata attcagcgaa tgctcaatcg ttgcccagaa
    15721 attcatcgca agagtccgaa tctggatttc cgccaaaatt ttcttctcac cgctgatcag
    15781 ctgtacagga tactcgatca ccagatgata agaacggtag ccacttgctt ttttatttgt
    15841 gatgtaatct cgttcttgga gaactttcat gtcttttctt ttacggatga tctcaacaac
    15901 ttgatggata tcttctacaa actgacacat gatccttaac ccggcaatat cagacatttc
    15961 ttcttctaaa cgtcccattg gaatatgccg taattgtgat tttgttacaa tactatcgac
    16021 tggcttgact ctcccagtga caaattcaat cgggacatgt cgattttgtt cacggaactg
    16081 tttgcggatc ccccgcagtt tcactttcaa ttctgataca gtttgttcat atggtgctaa
    16141 aaatgcttcc caatctcgtt ccatagctga tacctctact tcattaacat tcattgttta
    16201 ttctaccata tatgcaaagt tttttcgtac tcttgattta tgcattgacc aaaaaaagaa
    16261 aattaggcaa aatagacaaa aaggagtcga tcaaatgaca caaatgcttg aaatcgaata
    16321 taaatctatg ctgacaaagc aagagtacga gatattcatc gctcactatc aattaacaga
    16381 taaagatttc cgtgtacaga ctaatattta tttcgatacc gttgatgaac agctgaagaa
    16441 acaaaattgt ggcctacgga tccgtttcgt tggtcatcaa gctgaatata cattaaaaac
    16501 acctgctgaa aaagggcgtc tcgaaacaac ggataccttt ataacagaag aagcagaagc
    16561 atttatcttc gaaaaacgtc tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt
    16621 gaatatcgat cctgcttcat taatccaaac cggaaagctt acaacaaaac gagctgaatt
    16681 ccctattgaa gaaggacttt tagctattga tgaaagctgg ggagacaatc tacacgattt
    16741 tgaactggaa ctagaggtag gggatgcatc tgtgggaaaa attgctttta taaatttttt
    16801 aaaacgtttt tctttacctt atcgtcctgc taagaacaag atccaacgta tgcttgaagc
    16861 taaaaattag aaacctttca ttttcacaag gcacttgagt tcattttttt acttttttct
    16921 gttaagttat aggatgtgaa agaatctagt cactagttgt atagaatcta ttttggggga
    16981 gattcatatg atcgaaatct atttatttgt taatccttta ggggaatact gtttcgaaat
    17041 ggaacaacag ttattacaat ttatcgaaga agagtacggg acaccatcaa aagaaaagat
    17101 gcaatttcgc tttcttcctt tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa
    17161 aggaatttct cagaacaatc ttgaggaacg taatcactta ttctcaatga cttattctgc
    17221 agcattggac tgcaaagcgg cgcagtttca aggaaagaaa aaaggccgcc gttttttgat
    17281 caaattgcaa gaagcagtcg gctgtaatga cataccatat tctcaagaac ttgctgaatc
    17341 catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt
    17401 cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca
    17461 cccttctgca gtcgtttaca attatgcctg cgaccgtgat tttggtgttt tagtagaagt
    17521 aaaaggtaca agtagccttt ggagaatcac agagctgtgt aaaacaacag ataactcata
    17581 tcaagttttt aatggtgaac atttacatac aaatgattct cagttccatt caagaactag
    17641 ccatttacac ttgctttcta attaa
//
LOCUS       DBNIBO010000046        17548 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.049, whole genome shotgun sequence.
ACCESSION   DBNIBO010000046 DBNIBO010000000
VERSION     DBNIBO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.049"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            85..972
                     /locus_tag="AC1Y77_001299"
     CDS             85..972
                     /locus_tag="AC1Y77_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIQ1574888.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="AC1Y77_001300"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="AC1Y77_001300"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIQ1574889.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="AC1Y77_001301"
     CDS             2274..2720
                     /locus_tag="AC1Y77_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIQ1574890.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="AC1Y77_001302"
     CDS             2993..4345
                     /locus_tag="AC1Y77_001302"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HIQ1574891.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="AC1Y77_001303"
     CDS             4516..4803
                     /locus_tag="AC1Y77_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HIQ1574892.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="AC1Y77_001304"
     CDS             5029..5472
                     /locus_tag="AC1Y77_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1574893.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="AC1Y77_001305"
     CDS             complement(5478..6662)
                     /locus_tag="AC1Y77_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HIQ1574894.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="AC1Y77_001306"
     CDS             6930..7628
                     /locus_tag="AC1Y77_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIQ1574895.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="AC1Y77_001307"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="AC1Y77_001307"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIQ1574896.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="AC1Y77_001308"
     CDS             complement(8438..8971)
                     /locus_tag="AC1Y77_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574897.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="AC1Y77_001309"
     CDS             complement(9268..11649)
                     /locus_tag="AC1Y77_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HIQ1574898.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="AC1Y77_001310"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="AC1Y77_001310"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIQ1574899.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="AC1Y77_001311"
     CDS             12350..12889
                     /locus_tag="AC1Y77_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HIQ1574900.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="AC1Y77_001312"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="AC1Y77_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HIQ1574901.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="AC1Y77_001313"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC1Y77_001313"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="AC1Y77_001314"
     CDS             13996..15162
                     /locus_tag="AC1Y77_001314"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIQ1574902.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="AC1Y77_001315"
     CDS             15208..16704
                     /locus_tag="AC1Y77_001315"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIQ1574903.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="AC1Y77_001316"
     CDS             16785..17468
                     /locus_tag="AC1Y77_001316"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIQ1574904.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         5933 a         3019 c         3631 g         4965 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttac
//
LOCUS       DBNIBO010000047        17525 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.050, whole genome shotgun sequence.
ACCESSION   DBNIBO010000047 DBNIBO010000000
VERSION     DBNIBO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.050"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(857..2092)
                     /locus_tag="AC1Y77_001317"
     CDS             complement(857..2092)
                     /locus_tag="AC1Y77_001317"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1574905.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            2659..3486
                     /locus_tag="AC1Y77_001318"
     CDS             2659..3486
                     /locus_tag="AC1Y77_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1574906.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(3759..4529)
                     /locus_tag="AC1Y77_001319"
     CDS             complement(3759..4529)
                     /locus_tag="AC1Y77_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1574907.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            4747..5814
                     /gene="ulaG"
                     /locus_tag="AC1Y77_001320"
     CDS             4747..5814
                     /gene="ulaG"
                     /locus_tag="AC1Y77_001320"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HIQ1574908.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            5832..6293
                     /locus_tag="AC1Y77_001321"
     CDS             5832..6293
                     /locus_tag="AC1Y77_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1574909.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            6313..7797
                     /locus_tag="AC1Y77_001322"
     CDS             6313..7797
                     /locus_tag="AC1Y77_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIQ1574910.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            7840..8139
                     /locus_tag="AC1Y77_001323"
     CDS             7840..8139
                     /locus_tag="AC1Y77_001323"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1574911.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            8154..8792
                     /locus_tag="AC1Y77_001324"
     CDS             8154..8792
                     /locus_tag="AC1Y77_001324"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HIQ1574912.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            8797..9657
                     /locus_tag="AC1Y77_001325"
     CDS             8797..9657
                     /locus_tag="AC1Y77_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIQ1574913.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            9647..10357
                     /locus_tag="AC1Y77_001326"
     CDS             9647..10357
                     /locus_tag="AC1Y77_001326"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIQ1574914.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            10589..11023
                     /locus_tag="AC1Y77_001327"
     CDS             10589..11023
                     /locus_tag="AC1Y77_001327"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1574915.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(11120..11419)
                     /locus_tag="AC1Y77_001328"
     CDS             complement(11120..11419)
                     /locus_tag="AC1Y77_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574916.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(11688..11882)
                     /locus_tag="AC1Y77_001329"
     CDS             complement(11688..11882)
                     /locus_tag="AC1Y77_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574917.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            complement(12110..12871)
                     /locus_tag="AC1Y77_001330"
     CDS             complement(12110..12871)
                     /locus_tag="AC1Y77_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIQ1574918.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            13191..13436
                     /locus_tag="AC1Y77_001331"
     CDS             13191..13436
                     /locus_tag="AC1Y77_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574919.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(13620..14369)
                     /locus_tag="AC1Y77_001332"
     CDS             complement(13620..14369)
                     /locus_tag="AC1Y77_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1574920.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            14555..15259
                     /locus_tag="AC1Y77_001333"
     CDS             14555..15259
                     /locus_tag="AC1Y77_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIQ1574921.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            15299..15856
                     /gene="hxlB"
                     /locus_tag="AC1Y77_001334"
     CDS             15299..15856
                     /gene="hxlB"
                     /locus_tag="AC1Y77_001334"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - 6-phospho-3-hexuloisomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HIQ1574922.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            15864..17003
                     /locus_tag="AC1Y77_001335"
     CDS             15864..17003
                     /locus_tag="AC1Y77_001335"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HIQ1574923.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            17015..17446
                     /locus_tag="AC1Y77_001336"
     CDS             17015..17446
                     /locus_tag="AC1Y77_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1574924.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
BASE COUNT         5804 a         2745 c         3517 g         5459 t
ORIGIN      
        1 caccgtttat tcggaatatg ggactgtgac aaggcttttg tcacagccct tttttacata
       61 aagattatta ttgttttaac tgttttggca gaaagaagga ggcaaaaatg aaaattttat
      121 cagcaatgga ttaagaatta gtcgatctac taccaaagtg atcaaggcaa atatagattt
      181 gggagcttac aaccatatag taggggctgc agtatggtgt atgaatggta atatgtatga
      241 tgagagtgtt tattcaaatc atgaaacgtg tatagaaata attgcgctgg gaatagcaat
      301 cagtcatggt gaacgtgact tttagtttat cgttgctgac gacatagaac attctgacat
      361 gatctactca gcatgtgggg attgctggca gtttatatag gagtatgcat cggattgcga
      421 gatcattgtc tcaacagaag agggaggttg attatcaaag ataaagtggt aaatcctaca
      481 tttgatttag taattgttga agggactagc agcacaaatc tctacaaagc gatgcttcta
      541 gatctatatg acacatttta agaaaatgta tttgcctaat tattatggtc tttcttttga
      601 agaaacgttg agatgatcca accagagaaa ggtgtcaaag aatttgacta agaataaatg
      661 gcagacgcta cctatgctca tttggattgc gtcagatctt ttattttaag gatgattttg
      721 tgttttttag acaccgtaat ttaagtaact ctaacttttc aatccactat ttactttttt
      781 tgtattttca ctgtctgggt ctcgaatagc gaaattactg atgagtctct attaaaaaag
      841 ctttttctac ttgaaactat attgggtggt tgctcaggaa ggtttcgatt tcttgaattg
      901 cttgttgtgg accatttatt ggaaaaacgg cttgcagcgt gcgtgcctgc tgacgaatct
      961 tgggtgaaga aacttcgcgc agagcaatct gcagttttct ttcagtcact ggttttgtga
     1021 gggcgattgc gtttccgtat tttacacaat agtcaatatt acagctttgc tcaaattgca
     1081 tccccacacc gataaacggt ttgccggatt cacaggcagt ttggacggtc ccttccccgc
     1141 cgtggataaa agaaaaatca atgaattcat gcaaaagatg actagacaga tattctgtta
     1201 ttaaaagatc ttctgagtca gattgaatgt catcaagcag atagtcaata ggtgcaataa
     1261 ccgctagatc cggttgtttt tgtaagaaga acaaaatttt gcgtaacata cgcggattgc
     1321 ctgaactacc cattgcgaaa tagataatac gttttctttc ttgttgcttt cgctgtataa
     1381 aagttagcac ttcttcaggc aaggaagtac tgagcttagc aaaaattgga ccaacgattc
     1441 gataattttc cggcagctgt tcttcttcca aaaatagttt aggcgttgtg atgagattta
     1501 gatcagcatc catcatttgt aaacttgttt ttggtaaagg aagcatttct ttttgtgcag
     1561 cgtgtttgaa ggcattgggg atccatcggg tatttactat acgctttttt gtccagtgcc
     1621 aaaggggatt gcttaaaggg ccgaagaaac gtaactcgtg gggcatcagc gcatgctctg
     1681 attgaaaaaa aggttcactc aaagcatagg gtttaacata tactaaagga atctttttat
     1741 tgcgggccga taaaaaaatt gtcacattcg aacctgtcac aatcagtcga ggctcaaact
     1801 tagcaatcag actctgttca ctagtgatac gagaggcaac catttctttg gtaaaagggt
     1861 ttttaagtgt acgcaactgg tcaaatttta tgatttgttc ttcttgtttt ttcgtaagga
     1921 tcggcgctag taaatgatac tcaaagccac tttctgtgat caaaggagca aaagttttag
     1981 aaaagccaaa gaatacacag taataacggt ctttcatagc tttggcaacc tcaatcattc
     2041 gtgttgtctc agctaaattg aatactgcag gagcaaaaag aattgtcttc atgaaatcct
     2101 ccttattgga atgagtctat cccttcgttt cttactagta tactaaaaaa cacagcgttc
     2161 gagtagccta tgtatatttt gaatattaaa ctgtctctcg ctgttttcac aacttctaaa
     2221 tttaaatttt gtaatcaagt tgaaactcca attttcaaaa aaacacgcaa caattttttg
     2281 atcgatttat atgttgtgcc agataaatac gagaagttgt gtcattaaaa ataaacatga
     2341 gaacataaaa aataaaataa ttatattaat tgataggtgt gctgtcgtca ttatacacac
     2401 gtaagaaaga aatagttatg tttaaaaata ttcttagact ttctcgccat gacaattttc
     2461 actatgtgtt ataactgtaa acgataatta ttcagataac acaaaaagaa atcaaggagc
     2521 agatacgcca gttgtttaat ttgaatatta ttttaaaatt aaaattttta ttctgttcta
     2581 aacatttttg gttgtttaga actttttttt gtgtatgata taaaggagaa ataatttttt
     2641 aaagggaagg aaattgtttt gattaaagta gatggagcat tactaaaaga ataccgaaag
     2701 aaaaaggggt taacacaagt acaattagca gaaggcattt gtgaacaagc aactgtcagc
     2761 aatatcgaaa acaagaatat gtcgccaggc atcaatatct taagtgcgct ttgcaaaaag
     2821 ctgaatgtcg agatctccga tatattagta gaaacagaag aagacaaaat caggaaaaaa
     2881 ataaatgaga ttcaatcatt gaattctgta tcaaaatacc aagaatctta taaaatttta
     2941 caaacgatcg atcaggaaaa agtaagtaat tctgatcttt taaatcaaat tttattttat
     3001 caaggactgc ttgatcatct ttatgcgaaa aaaccagatg cagcattatt ttattttaat
     3061 caagtgttgg aacaaacgac cgaaacagat atccatcact tacaagcagc tgcaaacgtg
     3121 gcaatgatct acttgtcaaa aggagaatta gattttgcga aagtttatgt ggagcgtaca
     3181 cttaaaatat tgagtgagac tgattttgat aatctgatgg tctgcattgt atattatgat
     3241 atcgccacct attaccgtaa aatcgaggat tatgataaag caatccaatt atgtgagaag
     3301 ggtattgagt ataataagaa acataaatcg acttatgcat tggaatactt gctttatgaa
     3361 attgcttctt gccataagca attaggagaa gatgattatt tagaaagata tatggacgca
     3421 aaaaaaatag cgcgtttcaa cggaaatgat tacgcggtaa aggtgattga aaacgacctg
     3481 aaataaagtg gttgtgaaat cagtgttctg atttgaatac cgtttattcg aaaaaaggag
     3541 gtgtgacttt tgtcatacct ccttttttcg atattgtttg attcctattt aggcaacata
     3601 taacggttat aaaagaagca atagattatg atggtatcga ttggatggac acatcttggg
     3661 attcctttat caatttgagc gtagcgtgtt tttcatttat cgataaattt gttattcatt
     3721 tttttgataa tcctatttac atatttacgt cctggttctt aaaatgaatc tacttgaatg
     3781 gtttttatcc ccgtatttct gatctcctct aatacttttt cagaacaaaa tttatcagtg
     3841 atcaaataat caaagactga aatatcagca atcttgaact ttgatgtcag tccgattttt
     3901 cgatgatcag cgactaaaat cttacatttg attgtcttgt tgaccatcat ttcgttgatc
     3961 gttgattcat ttaatatctt tgttgtgacc ccatgtacta catcgactcc gctacagcca
     4021 atgatacaaa tgtctgcatt gactgaagaa atcgtattta atgcgatgtc gcccactagt
     4081 gcttcttttg gaaagcgcat ctctccacca gttaaaatat aactacaatt atctttgtgc
     4141 ggagagtaat tgattttcac gttgtttgta atgactgtca aatggttaga ttgaagaaaa
     4201 tccagtgttt tcaacgcagt cgagctagaa ttgataaaca gcgtatcata atcgttaatg
     4261 tatttactag cttctctggc aatcgcttgt ttaatccttt caggaccaga ttgatcaaaa
     4321 tcagtctggt ttttgtaatt ataagaacaa taaccgtaat tgcgtatgat ctcgtttttt
     4381 tcttctaata aatttaaatc tcttcgtatc gtactaatag atacatcaag ggattcagct
     4441 aattcgaaag ttgatagttg ttcagtctgc tgaagcagtt ccaaaatttc tttttgacgt
     4501 ttttcaatat tgacaagtga gtttttcatg acttttgcct cctgagggac atcttgatcc
     4561 atggatttat ttgactagtt gttcatttaa acattcatat ctgtctttat tatactgaaa
     4621 caatcattga ttactgaaag aaaatcatat aaaaaacaaa cggtcatata aaagggtttc
     4681 catggatgct gatacacatt aatatttact tgtaagttgg aacaacgata tggggggagc
     4741 aagcaaatga gtaaaactgt caatgaagta acaaaagaaa agtggttagt agaaacattt
     4801 cctgagtggg gcacatactt gaatgaagaa atcgaaacag aagtagtgga tcctggaact
     4861 tttgctatgt ggtggctggg ctgtacggga atttggctga aaagtcatga aggaacgaat
     4921 attttatgtg atctatggtg tgggacggga aaacgtagtc atgggaatgg catgatgaaa
     4981 aaaggccatc agatgatgcg tatgagtggc gttcaaaata tgcagcctaa tttacgtact
     5041 cagcctttcg taatcgatcc ttttgcgata aaaaatgtag acgctttaat cgttacacat
     5101 atccattccg atcatctaga tatccataca gccgcagcag ttcatcaaaa ttgtcctgaa
     5161 gcaacattca ttgggccaaa agaagtggtt cgcacgtggc aaaaatgggg aattcctgaa
     5221 gaaaaaacga ttacagtaaa gccaggtgat gcagtcaaaa tcaaagacat caatgtagta
     5281 gctttagaag cgtttgatcg gacagcgttg gtcacttgtg aagatccaga agaggtatta
     5341 aaaggaaaaa tgcctcagga gatggatcaa attgcagtaa actatttatt cgaaacatca
     5401 ggagggaatc tttatcatgc aggagattca cattactcca atttatttgc caaacatggt
     5461 aatgaacaca aaatcgatgt ctgtttaggg gcctatggtg aaaacccacg agggataaca
     5521 gacaaagtta cttctgttga tatgctgcgc atggcggagt cattgaatgc aaaagtagtg
     5581 attcctgtcc actacgatat ttggtctaat tttatggctg attcaaaaga gatcactgaa
     5641 atttggaaat tcaaaaagga tcgcctgaag tatggattta aaccttatat ctggcaagta
     5701 ggcgggaaat tcatctatcc gacaaacaaa gatgatcttg agtttaattt ttatcgagga
     5761 tttgaagatg tcttctcgat tgaaaatgat acaccgtttc cgtcgttttt ataagaaaga
     5821 aggggagttt cttgctgaaa tacttttttg accatcaatt gattcattac ccagataaag
     5881 aaatcacgga ttggcgtgaa gctatcgccg aaagctgtct tgtattgatc gataaggaaa
     5941 ttattgatga aacgtatgtg gaggagatca tccattgtgt agaaaaatac ggtccttata
     6001 ttgtgatcgc acctcaagta gcaatgccgc attcttcaga aaaaagtgat ggtgtttttg
     6061 gaacagctat ttcatttacc aagttgaaac agccaatccg ttttgaaagt gaaggggagg
     6121 atgaggagaa aacggctagt ttgtttttta cacttgcagc aaaagatcca gaagaacatc
     6181 tagaaaatat ccaaaaatta tccgaattat taatgacaga tggattggtt gagcgtctca
     6241 tctctacgaa ctccatcgat gattaccaag ttgtgatggc agaatataat taattgatag
     6301 aggagaaaaa agatggggga atttttgtta agcatttgga cgtattttgc aacaaatatc
     6361 ttgacgcagc cagcatacct gattggtttt atcgtgttat taggctatat tttgctgaaa
     6421 cgcccgcttc atgaatgcat cgctggattc ttaaaggcaa cggtcggtta ctttattttg
     6481 actgttggct ctggtggttt ggtcaataat tttcggccga ttttggttgg actaaaggaa
     6541 cggttcaatt tggatgccat ggttacagat ccttattttg gacaaaatgc tgtggatgct
     6601 ggattaatgg agacatttgg ccgtaccttt ggagatgtga tgattttatt actgattgca
     6661 tttgttttaa atatcttatt agtccgtttt cagaaatata cgaaactgcg ttccgttttc
     6721 actaccggaa acgttcaggt tcaacaagca gctaccgctt tttggatttt actgttttgc
     6781 tttcctgatc taggtaggat acaagtattg atttttatgg gactgatttt aggttgttat
     6841 tgggcagtag cttctaactt aacagtagga atcacgcagg aattgacaga aggtgccgga
     6901 tttgcagtgg cacatcagca aatgttcgga atctttatat ttgcaaaatt agctgagtgg
     6961 atgaaaaagc gggatgaaaa gaaaaataga tcaatcaagc aagacaaaaa aatcgaagat
     7021 atcaaattac caggattctt gtcgattttc aatgagaata tggttgctac ttcgttactc
     7081 atgctatttt tctttgggat cattctgatt gtactaggca aagattattt gattcaagcg
     7141 caattcatgc aagaaggaca gagtttctta ttctacatta tgacgacttc tttaaatttt
     7201 gcggtatatc tggcgatctt gcaattaggt gttcggacat tcgtcgatga actaactcaa
     7261 tctttccaag gaatctcaaa cacaatttta ccaggagctg ttccaggaat tgacgtagct
     7321 gctacttttg gctttggttc gccaaatgcg gtaacgattg ggttcttgtt tggtgctttg
     7381 ggacagtttt tgatgattgg tttactgatt ttattgaaat caccaacgat cgtcatagcc
     7441 ggtttcattc cgttgttctt tgataatgcc gttatagcag tttatgccaa taaccgaggt
     7501 ggattcaaag ccgcttgcct gttcccgttt ctttcaggaa tcatacaagt aggcggttca
     7561 gcattattcg ctacatggat tgggatgtca acatatggtg gttatatcgg gatgttcgac
     7621 tgggcaacag cttggccatt gtttacagta atcatgaaag tcctaggtta tgctggaatt
     7681 gccgtagttg tcattggttt attggctatt ccgcagttac aatatcggaa aaatcctgag
     7741 ggttattttt tgattgtaga agactatgac acatatgcag aaaagcagag agtataaaag
     7801 aaaatctgat tagacaacac tcataatgga gggattaaga tgagaatttt agtatcgtgt
     7861 gcaaatggat caggaacaag cttgatgatg atgagaagtg tggaaaaagc aatgaaagaa
     7921 ctaaacgtac caattaccaa aatccatcat tgtgccattt cagagggaaa aagttcagct
     7981 ggccaatacg atgtagtttt tacgccagtc aattttttga atatgtttac tcaggcagag
     8041 aaaaaagaag ttacagttat cggtatcaaa aatgtgatgt cagccaaaga aatcagtgaa
     8101 aaatttgctg aaacaaagtg gtaccaaaaa aacaagtaaa ggaaggaact aagatgagca
     8161 ggccgaattt gcaaattgcg ttagatcaca atgacttaga acatgcatta ggagatgtta
     8221 tgaaagttgg tgatgtagta gatattattg aagtgggcac aattctttgc ttacaggaag
     8281 gacaaaaagc gattcgctgt attcgcagta tgttcccaga taaaaaacta gtcgctgata
     8341 cgaaatgtgc agacgctgga ggaacagtgg cgagtaatgt tgcaaaagca ggagcagact
     8401 ggatgacagt catttgttgt gcaacgattc caactatgga ggcagcacaa aaagaaatag
     8461 gtgaattgca agtagaactc tacggcaatt ggacttttga acaagccatg gattggcata
     8521 acatcggtat ccggcaggta atctaccatc aaagccgtga tgcgctgtta tctggtgaaa
     8581 cttggggtga aaaagattta tcgaagatca aaaaactgat tgaactaggc tttaatgttt
     8641 ctgtaactgg tggattaaat ccacacacat tacacttatt tgaagggatc gatgtgtata
     8701 cctttatcac tggtcgtggt attacagcag caaatgatcc gatgaaggcc gcacaaaact
     8761 tcaaagatga gatcattcgt atctgggggt aaggaaatgg ctcaaattgg gatttatgaa
     8821 aaagcattac ccaaagatat ttcatggaaa gaacgttttt cccttgtaaa agaaatggac
     8881 ttcgatttta tcgagatgtc gattgatgag acggatgagc ggttagcacg attagattgg
     8941 tctgaagaaa agatagctga gctgagagaa gagatgttca gctccggtgt acgtatccat
     9001 tcgatttgtt taagtgcgca tcggcgattt ccgtttggtt ctgctgatcc agaaaagaaa
     9061 aaagcagcga aacaactaat gaaaaaagca atccatttgg cgcataatct aagcgtcaaa
     9121 gtaatccaaa tagctggata cgatgtttat tatgaagaga aatcaatgaa ttccagagaa
     9181 gactttatcg aaggaataaa agaaagcgta aaggaagctt ctaaatatgg catcattctt
     9241 tccgtggaaa tcatggatga tccatttatg aactccattt caaaattttt agaaatcaaa
     9301 gaacagattc attccccttt tctacaagta tatccagacc taggcaattt gtccgcatgg
     9361 ccggaaaata atccggctcg agaacttgag aaagggatcg attatatcac tgctatccac
     9421 ttaaaagata cttaccctgt aacaaaagaa tattcaggcc agtttcgtga tgttcctttt
     9481 ggtagcggtt gtgttgattt tcttggatta ttaagacatt tgaaacgtct agattatgat
     9541 ggaacatttc tgattgaaat gtggagtgaa aaaagccaag actttcgaag agaaatccag
     9601 caggcaaaag actacttaag ttctaaattg aaggaggctg gttatgatgt cttgtgaaga
     9661 aaaaattgca gcaatgaagc aacgtgtcct tgaagcaaat ctggcattgc cgcgatacgg
     9721 attagtcaaa ttgacttggg gcaatgtgag tgaagtggac cgtgagctag gagtcatcgt
     9781 cattaagcca agcggtgtca gatatgaatg catgcaagca gatcaaatgg tggtaacaga
     9841 tttgtcggga aatataatag aagaagatag cttaaaacca tcttcagatc tccccacaca
     9901 tgttgttttg tatcaaactt ttgaagatat aactgctatt actcatactc attccacgca
     9961 ctccgtcatg tgggcgcaag caggacgcga tttgccagct tatggcacga ctcatgctga
    10021 tgctttttat ggtaaggttc cttgtacacg ccaattaacg aaagaagaag taagagaagc
    10081 ttacgaagtt catacaggga atgtgattgt cgagacgttt aaggaaagaa aattagatcc
    10141 aaacgaagtc cctggagtac ttgtctatgg acatggaccg ttcacatggg gggattcacc
    10201 tatgaaagca gtagaaaaca gtttgatctt agatgaaatt tgtctgatgg ccaaagaaaa
    10261 cgagctgatc aaccccaata tatgtgaaat cccgcaatat ttactagata aacattattt
    10321 tcgcaaacat ggaaagtatg catactacgg gcaatagaga tgctagggta agggtagaaa
    10381 attaaaagat aacacttgat agagagaaac agaggttgtg aagaaaattt atcacaacct
    10441 ttttatttat caaaaaggat gcattctaaa aggaaagaga gattttaagg aatcgagaga
    10501 atctttacag gagagttata tgtgatagat taaatagtca aagaactgta ttattagagg
    10561 gagaattgta taagaaagga agaaaaaaat gaatattgat tcccttatga tcgatgagat
    10621 ttctgaatta tataagagcg ttggttggac gaattacaca aaagacactg caaggttagc
    10681 tagagcattt gaacaatctg aatctctgat aaagcgtaat ggggaaggga agattatcgg
    10741 aatcgttcgt tggatcactg attttgcgac gatcgcattt attcaagata tcctcattca
    10801 tccgaggtat cagcgccaag gaataggcaa agtgcttttg aatgaagctt tggagaagat
    10861 cactagttat ggaccagtcc aaatcgaatt attgacagat gatactgaga aaacaaaaaa
    10921 attttatgaa tcagtaggat ttgttcaagt aaaggaaatg ggtggtattt cttatgtcat
    10981 agatactaga agaaaaaagg tgctttttgg taatgacaaa taacatataa taacgtacaa
    11041 aagaaaaaat tctagcttag ttctttagaa aaacaagagg atagacttag tatcactcaa
    11101 tctatcctcc aatgctcagc tattttattt ttttactcac ctctttatca aatacaattt
    11161 tttgcaaatt cgaactaata taaaaaatga acacatgatt tttttttaaa gcaaatgcta
    11221 tgcgataatt ttttttatta attttgactt tcatctcgta tattcctttt gccgaacgag
    11281 tacgcactgg ctttatacga aaaaaattat gctccaaata ctctgtcact ttgtgggaaa
    11341 ttgcttcttt atacatgaaa tgttcttctt gtggaaattg cttaagtagt tcactgtcaa
    11401 attcaacagt ataatccatg ttttatttcc tttctatact gatacattat aaaaataaac
    11461 aattttataa gtctctatat ttcatgatga atgacaagta tatttgattc atcttgatat
    11521 agctctacga gatctccaat tccaggatac atattgaaat aatcatatgg tatacaaaca
    11581 tttatttttt ttcaattgtc aacactgcgt cttcttcttc taattctata atcttagcaa
    11641 tttgtacgtc gcttttcatg tgtgatcttc ctttctatac attggattta cttttcgatg
    11701 atatctacaa cagctttgcc acaagtcagt tctttttcta gctcgttctt tatttttatg
    11761 gcacatggtt tggaaaaagg agcagcttct ttccatgaac caagtaaagg atattcgtta
    11821 ctgatacctt tcacatattg tttgtttttg acgccttttg tttttacttc tacataatac
    11881 atttgatcct cttctttctt gctgttttta gttaatattc aaatatatgt aagtgaaaat
    11941 gattatgtat ctatatcttt atgataaata aaaagaatca atttgaaagt ttttttcact
    12001 cgatataact aatttgaata atatatataa taaaaggcta ttgttcttaa aatataaata
    12061 gtatataaag agattgaagg taatgctcga atgaacatta ccttcaatct taatgatgct
    12121 tataactaac gtaataaaaa cctgaaacaa aaatagctcc gccgatgata tttccaatat
    12181 aaacggggat aaaattcgag agaaattcta accaagtcgc ttgaccttca aatatggcag
    12241 caggtatcac aaaacagttg gccacactat gctggaaacc gattgccaca aaaatcatga
    12301 ctggaaacca aatcgttagt actttgccgg ccgcatcttt tgctccatat gataaccata
    12361 acgctaaacc gacaaaccaa ttacaaccta ttccggagcc taatgcttgc caccaagaaa
    12421 cagcgatttt atgatcagca atatgaatca cttggtcgag atagacacct gaatgagtta
    12481 aaccaacaaa gtgtccaaag aaataggcaa cgaaaattgc accaataata tttgcaaaag
    12541 taatcactga ccaattctta accagctgtg agaatgtgac ttttttatcc ataaaggcaa
    12601 tcgccacagc catcatattt ccggtgatca attcaccacc gccaagaagg atgaccagca
    12661 ggccgatagg aaaaaccgag gcaccaacta aactagtcaa gctagccata gattctggga
    12721 tagaagcaga gactcgtacg tatgccaaat aacctagaga aatcatggct cctccgataa
    12781 aacctaaaat cagtttagct aagataggtt tatgtatttt ttttgttcca agatgaatac
    12841 tgagttccat aatttcttct ggtgtgtgca taatacttcg attcctactt tcttaatgaa
    12901 ttttcatgtg ataaaatata gtgaaaaaaa tcacattttc tttgttgatt atatcatctt
    12961 attatttata tgtgcatatt attgtaaaat tttctattta atcacatttg attctaaaaa
    13021 agaaacaaaa ataaaagtca tgtatatggt ggtcaaaaag taaataatat cagttatcaa
    13081 atttcctgta aattttcttg aaactacagc atattattac ttcaaaacaa tctacatttt
    13141 ttataaaaaa ggaaatgaat aactgaaaaa attattatag gagagaaaaa atgtcaaaat
    13201 ctaaaaaaaa tatcaccata attggtttag gcttaaccgt atatttattt acagaatttg
    13261 tttatagaca tgtgaattta tttgcagaac ctttatttgt attatacgaa tcaaattcct
    13321 caatcgtcag tgaacttgct aatttaatga tagcattgct gtttactccc gcgttgagag
    13381 tcataactat tttggtctgt ttagtgctat tgttttttgt taataaaaag aaataacgta
    13441 gtatttcaaa agacaaagaa gtgggatgat gattgctata gtatgttata cgtggtgcta
    13501 ggtaagtgag aataaacaaa aaaggaaaca cgcaataaag tttatttcaa aactttattg
    13561 cgtgtttctt ttttagattt tttatatctg ttgctttagt agttaccaat agttttcgct
    13621 taatcaatca aaacgatttt ttgtttattg tattctttac gaaccttttc aggaatttct
    13681 tctgaaataa gataggtaag gtcttcaatt ggaccgacga tatgattact gatggtgccg
    13741 aatttatcat tagttatcat cccaagtact tcagtagaaa tacgtatcat ctcacgttta
    13801 atgtcggatt cttgcatagt tggcacgctc gttccaacat ctagatctat gttataaata
    13861 cccataatat aaaggtcggc tcgaatgttt tgtagttgct tataagcttg taccccaata
    13921 ttgatcatag attgtttata aaaagtaccg ccaatattga taatttccaa attaggatgc
    13981 tgtgctaatg ctatggcaat cggcgggcta ttggtaatga ttgttgcacg aaaatcttgt
    14041 gggatttctt ttgctaaagc caagttagtt gtacttccat caatgatgat gacagattct
    14101 tcttttaaaa aagggagggc tttatgagct aatgcagttt tttgttcgct agctacagtt
    14161 tctcggtagt taaaattggt aattggcgga ccaacacgta aagcgccgct gtgtactctt
    14221 tttaagagac cttggctatc caaatctttc aaatctcgtc gaatcgtgtc ttcggataca
    14281 ttgaaacgtt gacttaaatc actagcgata actttttgtt ctgcgtttaa taaatctaga
    14341 attttttgat gacgttctaa tttcaacatg ttgcgtgctc cttcttattt attcttgttt
    14401 atcatgtcat ttatgcgttt ttatgtcaat aatttccgtt tttaatacac taaaaaggca
    14461 taaacaagca aaaacaaaat atttttatgc agttttatgt tgactagtat ttgttaagcg
    14521 gttacaatga gggggtaatt aaaggaggga atgtatggag aaaaaaatta ttttgtcgcc
    14581 ttcacttatg tgtggggatc tagcaaattt ggaaggaagc atccaagaac tagaaagtat
    14641 tgggatggat tccttgcata ttgacgtgat tgacggttct tttagtccca gtatgccttt
    14701 agggatcgaa acaatcaaaa gaattagaga aatcacttca atgaattttg atgtacacat
    14761 catggctaag gataacgata acgaattttt tattggagaa atgttgaaaa ttggcgtaga
    14821 gagcattact tttcattatg aaacaagcct ccatattgat cgatatatcc agctcatcaa
    14881 gaattctgga acaaaagtag ggatagcttt gaatccagca actccgttaa atgtattaca
    14941 atatatttta cccgagattg ataggatttg tttgatgctg attaatccag gttttgcaac
    15001 aaacaagtca gaaaaacaag tttcttacgc gctaaaaaaa attacagatc tttgccagtt
    15061 agtaaatgag aaagagttag cagtagatat ccaagtggat ggacgggtat cgttagaaac
    15121 gatcccggga ttattagatg caggcgcaac aaatctggta ttaggaagta caagtttgtt
    15181 tcttaaagga aatacattag cagaaaatca aaaactactt tatcaagcga tgaagcaatc
    15241 agttaggaaa aatcaataga tgtagtggtc ctgaataata gaaatggagg ggaaatagtt
    15301 gagttataag gcggcgaaac aaaatattgt aagagaaatt actgaaacat tagaccgtat
    15361 ttcttcaaaa gatgtggaac aattgataaa gaaaattgaa aaagcggaac gagttttttt
    15421 cgtgggggtt ggcagagtac tgttagcgct agaaggaatg gcaaaacgct tagggcatct
    15481 aggaattcag acagtagtcg taggacaaat cactgaaccg gcaattacag ataaagattt
    15541 attgattgtt ggttctggta gtggggaaag tgctttccca ctaactattg caaaaaaagc
    15601 aaaggaattt gggtcccaag ttgcccatat aggatcaaac ccagacagca caatgaaaga
    15661 atattcagat ctttttattc gtatacccgt acaaacaaaa ttagcgttac cagaggaaat
    15721 ttcttctata cagccaatga ctagcctgtt tgaacaaagt gttcttttgt taggagatag
    15781 tattgcttta gtgatgatag aagaaaaagc aattgatatg tcaaagttgt ggcaatacca
    15841 tgctaactta gagtaggtga aaaatgaaag cggtacattt tggagcaggg aacatcggca
    15901 gagggcttat tggacaaatt ctacatcaga atggattcga tctatgtttt attgatacga
    15961 atcctgaact gatccatcaa ttaaatcaag ataagggtta tactatcgaa attattgatg
    16021 aaatgaacag gagtgatttc attgatcatg ttgtggcact gaatagttta tccgagctag
    16081 aagaggtgat tacagcaaca aaaaatgcag atatcttaac aacatctgtt ggcgcaataa
    16141 atttagtaaa aattgcaccg atcattgcgc aagccttaag taagcgattt gaacaaaagc
    16201 atagcatcaa tatcgtagct aatgaaaatg tgataaatgc aagtgatgtg ttgaaaaaag
    16261 aaatttattc tttgctgtca gaaaaagaac aagagaattt tgatgaatat gcatattttg
    16321 tgaatacggc aatcgatcga caatctttag gaaaagttct tggaggaaaa atgatctctg
    16381 ttgtcgaacc ttattatgaa tggataatgg atcgacagca gttagatcca actacatctt
    16441 ttgatatgaa aaatgtaaat ttggttgatg atatggagcc ttacattgag cgaaagcttt
    16501 atatcgttaa tgctgctcat gcggcagtag cttatttagg cgcactgaaa ggatataaga
    16561 cgattcaaga agcaatccaa aatatggaga ttttcaatct agtcaagcaa tttttaatgg
    16621 agaatataga gtactttgta caaaaatatc atttttctag agaagaatta acatcttttg
    16681 tggaaaaaac attaaaccgt catgggaatc agaaactctc cgatgaaatt actcgtgtag
    16741 gaagttcccc tattcgaaaa ttaggcccta atgatcgttt aattgctccc atagtaaaac
    16801 tagattcata tcatttacct tacgatgcag gaataaaagt aattgctgcg ggatatcatt
    16861 atcggaatct tgctgatcca gaagctgaac aactacgtcg aatgattgta aatgatgggt
    16921 tgaaagcaac gattaaaaag atttctcgtt tggaaggtaa gctgctgaat gaagttgtcg
    16981 ctgcttatga gtcccagatt tagaggagga gaatatgcta aaagaacaat tgtcaggaaa
    17041 tacaatgttt atggatgcga tcgattcatg gcaagaggca atccgagtaa gtgcgcgtcc
    17101 tttgatagga aagggaataa tagaggaaag ctatgtacaa gcgatgatac aaaatgtgtt
    17161 ggataacgga aattatatta tattactgcc actagtagca atgcctcatg cgcgtccaga
    17221 atttgggagc aaaggggtcg ggatgagctt tttatgttta gataagccag tcatgttccc
    17281 aagtgaagaa ccagtaaaat tcttctttac cttttcttct gattcgccaa agggacattt
    17341 agacttaatg gccaatcttg gagaattgct gagtgatgaa aaaatgtatc aaaaattatt
    17401 cgatgtgaag acagaaaaag cattgttaga tttgataaag aagtaaagga gagaagaaaa
    17461 tggaggtagc aaacaaaaaa aaacgagtct ccatgcgagc taaagttcaa gcgattggcg
    17521 gtttc
//
LOCUS       DBNIBO010000048        17466 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.051, whole genome shotgun sequence.
ACCESSION   DBNIBO010000048 DBNIBO010000000
VERSION     DBNIBO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.051"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1211
                     /locus_tag="AC1Y77_001337"
     CDS             <1..1211
                     /locus_tag="AC1Y77_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574925.1"
                     /translation="YQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQED
                     LLATFEERQQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRD
                     NAYSLCVEKINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQ
                     LNKLYQTTTEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQL
                     EIRKREQDLAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAA
                     VQTPVNNQANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTA
                     SIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNT
                     SESSESNEVK"
     gene            1211..3823
                     /locus_tag="AC1Y77_001338"
     CDS             1211..3823
                     /locus_tag="AC1Y77_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIQ1574926.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            3820..6198
                     /locus_tag="AC1Y77_001339"
     CDS             3820..6198
                     /locus_tag="AC1Y77_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HIQ1574927.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            6259..6801
                     /locus_tag="AC1Y77_001340"
     CDS             6259..6801
                     /locus_tag="AC1Y77_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574928.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            6879..7211
                     /locus_tag="AC1Y77_001341"
     CDS             6879..7211
                     /locus_tag="AC1Y77_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574929.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            7212..7805
                     /locus_tag="AC1Y77_001342"
     CDS             7212..7805
                     /locus_tag="AC1Y77_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574930.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7827..9521
                     /locus_tag="AC1Y77_001343"
     CDS             7827..9521
                     /locus_tag="AC1Y77_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIQ1574931.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            10209..12086
                     /gene="ltrA"
                     /locus_tag="AC1Y77_001344"
     CDS             10209..12086
                     /gene="ltrA"
                     /locus_tag="AC1Y77_001344"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIQ1574932.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            12217..12594
                     /locus_tag="AC1Y77_001345"
                     /pseudo
     CDS             12217..12594
                     /locus_tag="AC1Y77_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            12594..13799
                     /locus_tag="AC1Y77_001346"
     CDS             12594..13799
                     /locus_tag="AC1Y77_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIQ1574933.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            13815..14459
                     /locus_tag="AC1Y77_001347"
     CDS             13815..14459
                     /locus_tag="AC1Y77_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574934.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            14470..15276
                     /locus_tag="AC1Y77_001348"
     CDS             14470..15276
                     /locus_tag="AC1Y77_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574935.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            15298..15528
                     /locus_tag="AC1Y77_001349"
     CDS             15298..15528
                     /locus_tag="AC1Y77_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574936.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            16074..16349
                     /locus_tag="AC1Y77_001350"
     CDS             16074..16349
                     /locus_tag="AC1Y77_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574937.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            16391..16813
                     /locus_tag="AC1Y77_001351"
     CDS             16391..16813
                     /locus_tag="AC1Y77_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574938.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            16832..>17466
                     /gene="mobL"
                     /locus_tag="AC1Y77_001352"
     CDS             16832..>17466
                     /gene="mobL"
                     /locus_tag="AC1Y77_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HIQ1574939.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEG"
BASE COUNT         6515 a         2738 c         3572 g         4641 t
ORIGIN      
        1 aatatcaatt accccaaacc tatcaagcat ttttagagag taaagaagaa ttaatctcac
       61 agggtaaagg cacactttta gaacagttag agtctgtcca atcaacaaat tggcatgagc
      121 ttgcacaaga agatttatta gccacctttg aagaacgtca acaagaaaca gatcaagaaa
      181 ttaatgagta tgcgacggaa cagtcgtcta attgggagaa agtaaaagag gaactaaata
      241 aagaagaaca gcgagtgatt gaagaagaag tacaaaaagt caaagagcgg tacgaaaaga
      301 aacgcgacaa tgcttattcc ttgtgtgtag aaaagatcaa caattttact aagacgaatc
      361 aagaagcgtt atcgaaagaa aaagaaaact tgttaaaaga acgtattaaa gaacgaaaag
      421 aagagttcta ccgcggttta cgtacagata agctaaagat tagcaaagaa ttaaatgaac
      481 aactgaataa gctatatcaa acaacgactg aagttcttat ggaaaaacac gaacagatcc
      541 agcaagaatt acaagtacaa attccaaaat ggaaaaaaga gtatgaaatt caacaacaag
      601 aattgaaaga acaagaagaa aaacaacggc aaaaagaaaa agagcgtgaa caattagaaa
      661 taagaaaacg ggaacaggat cttgcggaac aaaaaatggc gatagaaaaa caacggttag
      721 agcaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa atggatacat
      781 tgcgacaagc acagttaaat tatctgacac atcctcaacc agtagctgct gttcaaacgc
      841 cagtgaacaa ccaagcaaat ttaactcctt caccccagcg ttcctttaac ggatggatgg
      901 tgggatgtat tgcaagttta agcttgcttc taggtggagg aacagtattt gcttttaatc
      961 atatcaatca atcttcacag gctgattctg tagccacaac ggcgtcaatc aaggaagaag
     1021 cccaggaaca agcaagaaaa gagtatgaac aacaattaga agaattattg aatcgtacca
     1081 atcagtcaac aacagagtca agcatggaaa atagcaaaga gagtgattct agtaaggcta
     1141 aagaaggatc aagcgattca actgtacaat caaacacttc cgaaagttca gaatcaaatg
     1201 aggtgaaata atgcgtgtta gagaaacaac gaagcgaaaa gacaaacgat tcaatttaga
     1261 aaagaatagg tatctttctc atttctatcg ctatactccc tcaggagaag agctaccaga
     1321 aaaacatgac aaacgcattt cgaaaaagat tttttatagt ggacttgtat acctcccttt
     1381 agagtattta gtaacaagag taccagaagt tttcaaccga ttggaaattg ttaagggaag
     1441 cttacaagga ataaatctgt ttcaattaca ggaaatgcca caggtctttc attttgattc
     1501 agctggtttt tggaatccat ttactcctac aactagtttg ggtacacgtt tagcaatttc
     1561 gacagtttgt gtggttccag ctgtacttat gggattgaaa atggacatgt ggtttcgtcc
     1621 tctagcagat ggacaaaaag gggataatcg attaatgacc ttaaaagaaa tccaacagat
     1681 ttatccgcaa atacctgaaa caaaaactac ttttccaggt tatggaggta ttccaatcac
     1741 acattacaaa aaatattggt acattcaaac agatactgtt aatacgtgta ttgttggtac
     1801 ctctcgatca gggaaaggtc agattgaagt gttagccacc attgacaatt tatctcgtgc
     1861 agaaaaacaa agttcaatgg tcgttaatga tccaaaaaca gaattatatg ttggtagtaa
     1921 agatgaacta gaagctagag ggtatgatgt atatgccttt aatatcaccg atccactgca
     1981 atcaatgagt gataatccat tggcactcat tgtgaaatac tggaagcgag gagacataga
     2041 caccgcaaca cagttaacca atacttttac gaatacgatt tattatgatg caaatgcttc
     2101 agacaataaa tactttaacg aaaatgcgca gaaagctgtc aatgcgctta tttttgcgtt
     2161 attggaacaa gcggatcaaa caggggatta ctccaaatta acaccaaata atctcgttga
     2221 actactttca gaaattggcg gttttaatta ttatccagat cctgagaatg aattcaagca
     2281 gctcaatgcc ttagatgagt tcatgaatca gttaccccct ggaaatatag caaaaaaaca
     2341 atatggggca acaaaaattg gatctgataa agcgaaaggg aatatcttgt ctacggcaat
     2401 tacaggattg aatccattta ccctaacgaa aattgcgaag atgacttctc aatcgaccat
     2461 tgattacaag tccattggtt ttccgaagta tttggatctc aaattacatg aaagcctatt
     2521 aaatcaacgc attgtcgtgg aattttatca taagggaaag aaatttcacg aggaacgagt
     2581 caaagttggc tatcgtggtt tttgtgaaat caactttgat tgtcatttag agcagggaga
     2641 tcatgtttgc ttaaaataca atcatcaagg aaaacaatat gttgcttggt atgagttttc
     2701 tcaaaaagta ttgaaagacg aagagggaaa aatcgtttat cgaaaaaaac gaggagaaac
     2761 tcatttgcca gaactagaaa aagaagccgt tctaacatta gatgagaaga aaagtaacct
     2821 attagtagag tctattcgga tgcactatac cgataaacca atggctattt ttatgttaac
     2881 gcctgattat gatccatcta accatatcat tgttagcatc tttcttagtc agttgtataa
     2941 agaattatct acgcaatgtg ttaagactaa aggagataaa tgtcaccgta ggattcattt
     3001 tattttagat gaatttggaa atatgccacc gatggacaac atggaaggaa tcatgacggt
     3061 tacggcaggt cgtaatatgt tatgggatct gtttattcaa tcttatcagc aactttatgc
     3121 gaagtatgga gaacaaaacg cagcgattat caaagaaaat tgtcagacac atatttatat
     3181 tatgtcgaca gatgataata ccatcgaaga attttctaaa aaagtgggga ataagacggt
     3241 tgaacaagag aacagtacca tcaattcatt aggtatgaat actcatatca atcgcaatgt
     3301 tgattcagat cgtatcttaa ctcctgaaag aatttctact tttttagaag gtgaaacgat
     3361 tgtactcgct cctttaaaac gacaagattt gaaaggtcat aaaatacggc cgtatcctat
     3421 ttttaatcat ggggaaacga atcttcctta tgcttatcaa tatattgaag atttcaatcc
     3481 atcgtgggat atcaatgata tagacataca ttgtacacat gcggacttgg atcttaaggc
     3541 tttacaaatt gattatgatt atttcctacc agatgaatat gctagaaatc tctatcatgg
     3601 actgcatgat caattagaag aaaaaaacga agaaaatatg ttttataaaa tcgttcaatc
     3661 ttttgagaat gagacggcta aaaaattgtt aattcaattt tacgaagaac aagcaacgga
     3721 tcgtcttcaa gaatatgttg aaaagttgat caaaaatgaa gaaatttctg aaatgattag
     3781 gaaacggctg ttattggaag ctgaaaaggc ggtgaaagaa tgatcattgg aggaattttc
     3841 tctggcatag ccgactggtg gtcaggttta ttcggagaag gggatgcaga tcccaaaacc
     3901 gtaacgaaag tcttacaaga ttatggtaat tatctgcagt atcaagatgt attcagttgg
     3961 atttgtaata cttttttatg gggattgatc aaagggttat ataatctcaa tactttatta
     4021 gaaaaaacaa tttatcaaag ctttgattta aaagacttat tgaacgccgc tggtgttaat
     4081 gaactatttc aaagtttgat tagtaaggta ttggctcttt tttgtgtcgt cacattaatt
     4141 tatttaggct tgaaattttt tatgagtaaa cacccaccga aagtcaaaaa tatcttggtt
     4201 aatttattta tggccatgat cttgattttt ggtggtagtt ctttaatcga tgaagggtta
     4261 aatatcagta aaaactttta tggagacgtc actgcagcaa ataaagcaga taaaacgggt
     4321 tcacctgctt ttcaaattat tcaaaataat gtgattgatg tcagtacatt actagcgact
     4381 gacccgaata aagtagaatc cttaccaaca gataaaagaa atgctttaac agcgaaaaat
     4441 ttcaaaacag cgaatatcaa tgggattatt acacctgatc aagctaagaa aatgtctgaa
     4501 tcaagtgata ttagtacagt acaaaaagat cggttaaagg cattacagta tcgactagag
     4561 ttagacgata atgggaaaga aatccctgtt caaattagtg atgaaggttt agcaaaatat
     4621 gtgtatacgt caggctatcg tcgatacatt agtagtccag ggattatttt ggctggggaa
     4681 tcttctttgt cggtagccta tttatttatt ttgtttacaa tcgttacttg cattattgag
     4741 ctagtattta aaaaattcta tttagtaatt gccgcgtcaa cggactttga gacaggacaa
     4801 cggatgaaaa gtgcgataga agatatttct caatccttct tgttattggc gtttacgatt
     4861 ttagaattac gaatctatac attaatgctt tcaggtatcg gtgatttgca tgcggtagga
     4921 aagatcaatg gattcttgta tgttgttgct ttaattgtgc taactattgc gctattcaaa
     4981 ggctctcaat cggttacgaa gattttcggt gttgatacga gtctgaaaaa cggcagcaat
     5041 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
     5101 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taagaatgga
     5161 tttggccatc gaaaagatat tacccatgat aacggggaag aacaatcaga cgataaagct
     5221 acaactgtac ctaatagtaa gaagacagtt tccgaaatgt tcgggaaagc gaaaaatggt
     5281 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagtt
     5341 gaagatctta cagaaaaagc caaagacacc ttagaagaga aattagacgg cacgagagct
     5401 aaagaatttg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
     5461 gcttcagcag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
     5521 gtgaaggcaa atacaacaag tgaaaaagaa aatcaagaag gtgcagcgat tccttcagcc
     5581 aaagatcacc aagatgagtt acattctaac gaaaattcga tcggagaaaa agattcagaa
     5641 gaaggacaac atggtcagtt accaaaaacc gatccagaac aatatcgtta taaatctaac
     5701 attaattctg aaaacgaaaa acgcatttca gatttaccaa aacgaaatca agaagtacaa
     5761 acggatgagg attccatcga tctgccaaat gataatggca tcgaagttgg aactgaaaaa
     5821 tcaacgaaaa ttggtttgcc taagacacaa aatttaccaa tgaaagatag tcgtttgaat
     5881 caattcaaag agaatgatca aagctttgtt catcaatcac ctgcaggtac tgttcaaatg
     5941 aaatcaagtc gtttgcctga aaatggaaat ctgacaactg tttcttacaa tttagctgaa
     6001 agtggtgaaa cagagaatgc gtccgttcaa aatattgtga caaaagaagc tgcacctaag
     6061 ccaatcaata aagttcataa tggatacaga ccagcaaacg tagcatttac tgcaccgaca
     6121 aaaataaatg tgacaagaga aactaaaaac aggaacaatg aattgaaaga acaactattg
     6181 aaggatatca aaccataaaa ggcctattta agggcctttt tctttataaa acaagtttaa
     6241 aatggaaagg agaaaattgt ggaggatatt ttaatagcta cgtttataac tgcactggga
     6301 gcatttttaa cgatatttgt ggtgactatt aaaggattta aagatacgga tagtttacaa
     6361 aaagatcatc agtctatttt agatggtcaa aaaacacata ccgaaagcat tattgtacac
     6421 ctttcagaag gattcactta tttgaaagat gctatgccca cattcacagt aaaattaacg
     6481 gaaatatttc attctgttga gcaaatatcg tcagaattga aagagcaaag acaacaaaaa
     6541 gatcgtcaac ttggctcact taattttagg caagaaacga tacaagaatc attggagaat
     6601 gtaaatcgat taaacgatga aatgcaacga gttcatttgg aaaatgaaga attacgcgca
     6661 caaaattatg atttaattca aaagaaaaga aatctggaac aaaaaattgt tgaattaaaa
     6721 gaagaaaatc gtcaattgaa ttatcaatta cgacgaggaa aaaatcgttc tcgtgattta
     6781 ggaaatgaat tagagttgta atatttagaa aaacgctgtg agaaatcaca gtgtttttta
     6841 tttctaaaaa tacaaaatat aaggggtatg gttatattat ggataaatac attcatatga
     6901 aaaatcctcc aagatttagt ggggaatgga agtttttcgc ctttttcttt ttagatctat
     6961 taacgcttgg tggctgcttt ttttgggcga aaatagtcaa taatcttgtc cacttaccac
     7021 tcttttttgc actcttgaat tatggattat ggcttgcgat agcgttagtg attgtttggc
     7081 gaccctataa taaccctggt caacgccaaa tttttgcgat tatcgaatct attttcttta
     7141 cggataagaa ccactatcat gcgttagatc ctaaccgaaa ttatcatggt acgtataagg
     7201 aggatgaata aatgcgagtc aaaggacaaa attcagttga taagaaatcc attttgcagt
     7261 ttggaaaacc agaagagcgc tctatcttgg acttatttcc atataaatcc tgtacagaag
     7321 agggagtctt aattactaga gaagatcatt ttcagcgtta ttatcgtgtc gtttccactg
     7381 acgtggaggg attaaatgaa caagagaaac tagaacgaat gaatcagttg accaccgtca
     7441 tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg gatctaacgg
     7501 aacaaatcgt gcaaaagcgg cagttattag ataaaaatcg tatggaacag tcactaggaa
     7561 gaaatctaca gcgattaaga aaatatgaaa agaaattagt agaggagatc caggaattaa
     7621 aacgggcaga acaagaacgt cctgacttaa gcttcttttt cgttattgaa ggaaagacaa
     7681 tcaaggattt acaaactaga aatcgtcagt taatgcgatc tagtggcatt ttagggttaa
     7741 aaccactcta caaaaaagaa ttgattaaga ttctttatcg aatgaacaac atgaatgacg
     7801 aataaaaaag atggaggaaa tgattcatgc tagatgtgat aaaaacagct ttttcaacgc
     7861 aaagtaatca aaaggtgatt gatccattaa aacaacttgg taatcgtgaa atagagattt
     7921 taaaatcaaa aggttatgat ttagatttct taaaacaagt gatgccacaa ggtgggttga
     7981 attttgatga agaatatgtg acctatggaa atggttattc acgatgtgtg acagtttatc
     8041 attacacaaa aaatcctact cttttttggt tggccaatct catgaacaat cctcatacag
     8101 tggccacact agatgttagc acagatgaaa agcaaaaagt gattgatcaa atcaaccgcg
     8161 cattagatga attaaatgat cgatcagata atgaacgaaa agcgactgac cgaaataaaa
     8221 gtgcggaaga atacctggaa ctagcggaat atgcccgaaa attgacttct aatggggaaa
     8281 tttctaaaca agtcaaagtg agagtacatg tttacggaaa tacgatccaa gaagtggatg
     8341 aacgggcgca ggaactgatc gaaaatctaa aagggatgga ttatcgtgcg gtgacttatc
     8401 tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa caagctgaat
     8461 ggtttggaaa tatcaaaggg caatcacttc catcgttaaa cattggcgga ggattcccgt
     8521 tccaccacca aacgctaaaa gatccaggag gcacacaata tggggaaacc atgacaggcg
     8581 gcccatttgt ctggaatgcc acgtataaaa atagtgtacg tttatctacc aataccttgc
     8641 ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg gaagcccatt
     8701 tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt cctttcttaa
     8761 aggaatataa cggtattatg gtgcgtttag acggatctga cggaatgatc aatcctttag
     8821 agatatttgc cacacggacg tatgatgaag catccagttt atatgacgat ggatcagtca
     8881 aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca cttagacaaa gtggtctatc
     8941 aggtacagtt agtgagtcct cagctaaaag gaacgatgaa agcagaattt aagacctact
     9001 taaatcgttt ttatgaagag tatggaacag taccacgcgg ctttacttcc tcaaattcgc
     9061 gttcaaatac tgatacgcaa gtcacaggga aagatccaga agcttatccg acgttcaaag
     9121 aatttttagc ctttttagga caactggaac taccaggggc ttcccaagag aaaaagaatc
     9181 ggaaagaaga aatggagtct atcgtggaat ccttatgtga gacttatggc atgatttttg
     9241 atggtcatag tacgattcga caccttgacc aacaacagtt ggtctttttt gacattgact
     9301 ctattacagg cctagatcga ggggtacaac aatgtcagat gtacatggcc atgacgttga
     9361 tttggaatca ggcattgatt caaggacgaa gacaacgacg cttaattaaa gaaaaacaaa
     9421 tggaagaaca agacttcaaa cgcttcttgg cgatgattga tgagtgccat aatggtgcga
     9481 cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta aaaactattg taataactct
     9541 tataacgaga agtcaaatga gtgggtaacg ccacaaaggg cttccactga tactccgaca
     9601 tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct cggtgaagtc ggctgaagga
     9661 taccgtaagg taaaagaaat agtgaatacc acgaaagttc tctagaggta aagggtgtat
     9721 ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt atgtacaaac gtactgacga
     9781 actagcgaat gtactgtccg aaaggcatag ctcctagaaa tgggagagac agtgagcaaa
     9841 aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg ttgttatgaa agaccatgct
     9901 gcgttacagg cgtaggagaa gttagggggc aaaagctagc atctaagtta aggcatgtgg
     9961 atagatataa gggaacaggg gaagccatca acatggagag tgcatactcg atgaagtggt
    10021 agtaagaaat atgtgaatga caaataactt actttagtga tggtgaaagt agagtcacga
    10081 ctgatgaagg ttctgtaatg gaactggagg aacaggctcg attcgtctgt aaaagtcatg
    10141 aaacccgttc aaccatggga ttctcgtatg actaaaaagg tcacgagcca attggagcgt
    10201 gataaaccat gacaaaagaa acaagtccaa aagataggaa gttgcggcat gcagaatatt
    10261 atggcatgac ggaaatcttc gataagatat actccaaaag tttgaaggga gataatttca
    10321 agaatctaat gcagattatc atttctgata acaacattgt cctagcatat cgtaatatca
    10381 agagaaatca cggtagtgtg acaagaggag aagatggagt aacgattaaa gatattgaaa
    10441 atctcaaccg taatgaattc gtagaaattg ttaggaagcg attcgcacat tataaccctc
    10501 ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa attaagaccg ttgggtattc
    10561 catcaatatg ggatagaatc gcgcagcaat gcatcttaca agtattagaa ccaatatgtg
    10621 aagcaaaatt caataatcac agctatggtt tccgcccaaa tagatcagca gaacacgcaa
    10681 tagcagattg tatggcccga atgaatcaaa gtcacatgga atatgtggta gatgtagata
    10741 tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat gagacaaatc tggtctatgg
    10801 gtatacgaga caaacagctt ctcttaatca ttaggaaaat gttaagagca cccgtagtac
    10861 ttccaaatgg gaagttgata tacccaacaa aaggaacacc acaaggcgga atacttagcc
    10921 cattacttgc gaacatcaat ctcaacgaat ttgactggtg gatagcaaat cagtgggagg
    10981 aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa tggagcttgg gataaaggta
    11041 atatctatca caagctcaga aagagtacaa ctctaaaaga aatataccta gtgagatacg
    11101 cagatgattt taaaattttc tgtagaaatt ataatgaagc gaaaaaagtt ttctatgcaa
    11161 ctaaaatgtg gttacaggag cgtctaaagt taccaatatc tgaagaaaag tcaaaaatca
    11221 caaatctttc aaaagaagaa agtgaatttt taggctttac gatgaaatta gtgaccaaag
    11281 gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc acaaagcatg attaaagtga
    11341 aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa tagcaaagat acctttatct
    11401 atattggagt ctataacagt atggttattg ggattcataa ttacttcaaa attgcgactc
    11461 attgtagtgt agcaatgcga aaaattgcaa gagaactcag accaagtttt gaaatacgac
    11521 tacgagaaca aggaatctca aaagaaggac aatacggaaa gtacgaagga tacaagcctt
    11581 acctacggtc aggacaaata cgatatataa gagggtttcc aatattacca gtagggtata
    11641 ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa taaatacacc gaagaaggta
    11701 gggaagccat tcataagaag caacaaaccg tagaagaatg gaagatatcg tggttgtgta
    11761 gcaatccagt gcttagtaag agagcaacga ttgaatttgc ggacaatcga atttcaaaat
    11821 tcatcgctca aaagggaaga tgtgcagtaa caggtgagga gcttatctta tctgaaatgc
    11881 attgtcatca tattatccct tatcatgaaa gcaaaagtga tagctatgaa aacctagtga
    11941 ttgttactga ggaggttcat cgggtaatac acgctacaca aagtgaaacg attgaggaac
    12001 ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa actcaatgag ttgagattaa
    12061 aagttggtaa cgaagaaata tcttaataaa tatttgtagg acgggaaaac agaatgtctt
    12121 ttatagacga gggaacgccg tatgcggtga aagtcgcacg tacggtgtgg actgggggaa
    12181 aagacggaga taatatcaaa ctcttaccta tcagtatcga ttaatccaga ttttattgaa
    12241 acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta aagtagaagc aatgactgtt
    12301 ttagctacac aatccccaca agaaatggtg ccagaaaata tgtcttctga taacttctcg
    12361 aaactgaaga aagtctttga attttcttct acgaagatct ttatgcgtat ggatgaatct
    12421 attttacgtc atatagaagg attagtgggt aaatcaatga cgaactcaga attagaatcc
    12481 attcctcaac aaaatcaagg agacgctgtg atcaattttg gctcaaaaga aaccattcgt
    12541 gttcatttta gtccgaacaa acgtcaatta aaattgtttg atggaggaaa ataatggaaa
    12601 accaaaatga aagtttaatc aagcagtatg ttaagcgtcg tgcaaagcgg cgcttatttt
    12661 tgtggttgtt tggtacttct gcaggtttga ttactatttt gatcactgtg ttcgtgaccc
    12721 tatttctaat acttgcggcg ggctcgattg ataattcgga ttcagattca agcagtggtg
    12781 gcgaagcttt tacaggagaa tattcggaag gcttaccgat ttataaagaa atcaaaggaa
    12841 gaggcccttt ttctgatgag attgcgcaat atgccgtagg tgcagcagtg aaatataaat
    12901 tattaccaag tgtgatcctt tcgcaatacg gttacgaatc agcgtttggt acatcggcgt
    12961 ccgcaagaaa tgatctcaac tattttggga ttacttggtt tgatggttgt ctgtttccta
    13021 aaggaactgc acgaggaatt ggtggcattg aaggtggctg gtacatgaag tttcccaact
    13081 caaaagctgc attttcctat tacggcttta tggtagccac acaatccaat tttaatgctt
    13141 gtgtaggaaa taaaagccct ggcgcaagct tattgatctt aggacgaggc ggttatgccg
    13201 ctgcaggaat tacggaagat agtccgtatt atacaggttg tatgtcaatt atcacttcta
    13261 ataaattaac cgaatatgat gaattcgcca tcaaacattg gggtgaaggt ggcaataaca
    13321 acggcacgat tactggtgaa tggacgaatc cattccctgg cagcagttta gataaaagtt
    13381 ccttcagcgg tggtcaatta tttggaacga atccaggcgg tgaattccgt ccgaacggct
    13441 tccatgatgg attagatttt ggttcagttg atcatccagg aagtgagatt catgcggttc
    13501 atggcggaaa agtcgtgtac gttggcaatc ctggtatttc aggtttgggc gcgtgtgtga
    13561 tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga atttgcaaat agtacaggaa
    13621 attctcgagt gaaagtaggc gatcaagtga aagttggtca agtaattggg atacgagata
    13681 cagcacactt gcatttagga tttactcgaa tggattggcg tcaagcacaa ggacatgcat
    13741 tcattgatga tggtacatgg atcgatccgt taccattttt gaatagcagt aaaaaataga
    13801 tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt cttaacagct gcaattagcg
    13861 ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa agaggaacaa gcagcaaata
    13921 cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa gctcaaaaaa aattcctctg
    13981 aggcacaggc gaagatcgac aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg
    14041 atcagctgaa gcaacaagat ggcacaaaag caaaagagga agtaactgaa gcacaaagaa
    14101 agacggcaga agagttcggt caattggcca ttgataaatc attaagcaga aatgaattaa
    14161 cggaaaaact aaaagatgtc gcaacccaag aagtgatcga caaattaagt cctacagacg
    14221 atgatcacca acatgatgat tacggaagtt actcctttac attaggtgac gtgcaaactt
    14281 acgctcaaac aagcagtgta aaagaggaac aaaattttgt cgtatttgtt gattatacaa
    14341 tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat aaaaggtgga ttaactgtca
    14401 ctgaaaaaca agtagatggt caatggaaag tcatagactt tagttatttc actcgttagg
    14461 aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt tcaaaaaacg aaaaagttat
    14521 cttagatggc tgggaatctt tatgattctc ggctttttga ttcttgctag tccgatttat
    14581 acgaatcaga aggtagatat accagatgag gtaagcacga acgccttttt aaacacgaaa
    14641 gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc aattagtttt tgaatttgcg
    14701 atcgatgatg aagatcaaag cttgttaaaa gaactggcta atcttaatta tcgcgtagaa
    14761 gtaaagacag ctaaagggga ttatcaagca atacgagcca aagtgattaa ggtgtctgat
    14821 gattattttg cagtgaagct aacgcatgta ccagaagaat atatcgccat gcgtttaacc
    14881 attattcctg aaaaaattga tccaaaagtg gatatgcaag aaccacaaga tctgatttat
    14941 tacgtgcacg aagataaggt taaagatcaa gtgaaagatg aagattatga acaacacgcg
    15001 atcaattaca aagtgaaagg atacaagaaa gaacaaaaag cagctcaaaa acaaatggaa
    15061 tctttccaag caaccattga tttgaatgaa gaattagtga agaagctaaa agatcaattg
    15121 ccttatcaag tcgcagatga tcaggaaaat actgaaaaca agattaatag ctaccagcaa
    15181 gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag agatcattca aaaacttcaa
    15241 gaaaaaattg atcgtatgac aaaagaaaag atttagaagg acaggtggag tgaaaaaatg
    15301 aagcgaacaa acttggctga caaagatact ttcgctgagc agaaagtatt gattaaagcc
    15361 tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag tgaatttatc taacacaaca
    15421 atatctgagt tgaagaatgg tcgaaagaat ttagatactt taaaattcag atcatttgaa
    15481 gctctatatg tagctgctaa aaaatatttt acagctgata acaaataaaa aacaggagta
    15541 ttttatgtga aaaaagtata taaaaaatgc ttatgaatca gctatatcac cttaaccatt
    15601 tgttaatgag atgggagaaa acattaacca cgattctaac gcaaacatca agcacataaa
    15661 acatttaaca ctcctccttt ctggctgaag atttcaacca aacaggaaga cagcgtatca
    15721 attatagcat acactttttc ttaaattcat ttttcatatg acaaaatatt tccttctaat
    15781 cgacaaagtt agaaggattt tttattgtct aaatacacaa ataaccttaa tttttaaaga
    15841 gcataaggaa acgtgatatc actttctcac gaaaggtgtt aggtaggctg tgcctaccac
    15901 gtttctaaga aagataaatg atatcaaaat tttggatttg tgtgtagatt tgtgggtagg
    15961 atgaaagcga atgtgggcag taaaataagc gtttgtgtgt agcaaaatca aaaaatgtgt
    16021 agggatatgt gtagctacac atatccctac acataataaa tttggaggaa aatatggact
    16081 tattaattag aaatattgaa gaaaagtata ttaataaaat tgacaaacgt tgtgaagaac
    16141 ttacggtcaa gactggcaaa aaatggagtc gaaatcaata cctaaaagtt ttgatcgaaa
    16201 atgattttga tcacgctctt ctgaattata aaaaagacca atttgatcgg ttgttagaaa
    16261 aatttgtgga tattcaaact tataatacga aaactttaga agaatatatt gcaacaaata
    16321 atcaattaat tggtttgtta attgaataat aaaaatagta ttgtgaattt ataaaaggag
    16381 gaaatcaatt ttggcaaaag caacaagaaa acatgtatat ctttacgata tgcatatcga
    16441 agtacttgat gaaataattt ctactcatga agatatatct aattatagtg aagcagtcag
    16501 acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa ataaatccaa ttcaaattaa
    16561 attgaatgca atgggaaaag aaatttctat tttaaccgaa atggtttcag agtttgcgga
    16621 tcttcatttg aaagatacag gaatactaat tggtacaaat tcaactgttt atcaagaagc
    16681 agaagaacgt gtggaaggaa aaattaaatc caatcaaacc aaaaaatatt cttctaagtt
    16741 aaagaaacca gtgaatgaat ctgttcatac cagagtagat ccatttgatc caacgaagat
    16801 tctaaaaaga taaaagtaga aggagaacgt gatgaataaa tcgcctgcag ttattcttac
    16861 ttgtcaattt accttgccca ataaaaaatc tttttcaaca tacattgatt atatgacaag
    16921 agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa gaagaacagg aactgcaacg
    16981 aatcaagaat gctttagagg atttttatat tcctgaagga gaaacttttt cggaaagtaa
    17041 gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa tcgttgatgg aacatggaat
    17101 caattttgac aagttagagg aacaagattt taccaaatat ctttcttata tgacccgtca
    17161 ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa caaaaagaat accaacggtt
    17221 gaaccaagca atgaaaaaat atcaaacaga gccacataaa aaagcttcag gaaatcttcc
    17281 tggagttttt tctatggctt cagacgaagt gaaaggaagt gatctgaagg agatcaaaca
    17341 aatcttccaa agaggacaac aaaaaggctc aatcatttat caagatgtta tctctcacga
    17401 caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa acagatacct tagatgaaga
    17461 aggttt
//
LOCUS       DBNIBO010000049        17164 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.052, whole genome shotgun sequence.
ACCESSION   DBNIBO010000049 DBNIBO010000000
VERSION     DBNIBO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.052"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(36..845)
                     /locus_tag="AC1Y77_001353"
     CDS             complement(36..845)
                     /locus_tag="AC1Y77_001353"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1574940.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1138..2574
                     /locus_tag="AC1Y77_001354"
     CDS             1138..2574
                     /locus_tag="AC1Y77_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIQ1574941.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYFWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2744..3940
                     /locus_tag="AC1Y77_001355"
     CDS             2744..3940
                     /locus_tag="AC1Y77_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HIQ1574942.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4002..4187)
                     /locus_tag="AC1Y77_001356"
     CDS             complement(4002..4187)
                     /locus_tag="AC1Y77_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HIQ1574943.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4418..7732
                     /gene="dnaE"
                     /locus_tag="AC1Y77_001357"
     CDS             4418..7732
                     /gene="dnaE"
                     /locus_tag="AC1Y77_001357"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIQ1574944.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7900..8862
                     /gene="pfkA"
                     /locus_tag="AC1Y77_001358"
     CDS             7900..8862
                     /gene="pfkA"
                     /locus_tag="AC1Y77_001358"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIQ1574945.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8936..10720
                     /gene="pyk"
                     /locus_tag="AC1Y77_001359"
     CDS             8936..10720
                     /gene="pyk"
                     /locus_tag="AC1Y77_001359"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIQ1574946.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10755..11657)
                     /locus_tag="AC1Y77_001360"
     CDS             complement(10755..11657)
                     /locus_tag="AC1Y77_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIQ1574947.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11843..12412
                     /locus_tag="AC1Y77_001361"
     CDS             11843..12412
                     /locus_tag="AC1Y77_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIQ1574948.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12470..12649
                     /gene="rpmF"
                     /locus_tag="AC1Y77_001362"
     CDS             12470..12649
                     /gene="rpmF"
                     /locus_tag="AC1Y77_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIQ1574949.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12819..14240
                     /gene="gndA"
                     /locus_tag="AC1Y77_001363"
     CDS             12819..14240
                     /gene="gndA"
                     /locus_tag="AC1Y77_001363"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HIQ1574950.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14433..15119
                     /locus_tag="AC1Y77_001364"
     CDS             14433..15119
                     /locus_tag="AC1Y77_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1574951.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15116..16621
                     /locus_tag="AC1Y77_001365"
     CDS             15116..16621
                     /locus_tag="AC1Y77_001365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIQ1574952.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5551 a         2982 c         3595 g         5036 t
ORIGIN      
        1 gtcacaacct cttttttgtg gaatctaatt ctctattata gtttaagtag tttttccaga
       61 taaacagcta aaccgtcttc ctgattgctc aaaggagtga catcatttgc aatgcctttg
      121 agttggtcag taccgttagc catagctact ccccaacctg cataatccaa caattccaaa
      181 tcattatgct cgtcaccaaa ggcaatgacg tctttttgat cgatagacag gtagttagca
      241 atctcttgca cgccttttgc tttttggatt ccttttgaaa caatctctaa gatggcagta
      301 ggtccacccc atgtccgaac atcgacataa ctgccaaatt gacgtgttaa ttcagcagat
      361 acagctcctg caaaacgttt gtctgttcgg acaagcaaag atgtaggatt tgtcgtcaaa
      421 tttttaggag aaagcaaatt cttctctcct ggacgagaag aagcaaaaat tttttcatca
      481 aagaaatcaa aactgtcaat aaaaaaagta tcacgatttt cagcagcgat aaaatctaaa
      541 tttagctgct ttttttgcga taagatttca aaagcgattt cacgattgat caacgtttct
      601 ctttcaccat cccagtgctg attagggatg tgtactaatg cgccgttaaa attaaccatc
      661 ggtgttgtta attctaattg ttggtaaaac tgatggctca ttcggaatgg gcgtcctgta
      721 gcaatactga catgatgacc ggcattgatt gcttttttta gagtctcttt tgttttagat
      781 gtaatcatcg aatcttgatt gagggtagtt ccgtccagat cgatggcaat taatttttta
      841 ttcaataagg gtccttcctt cctcaactag catttgtttt agtctagcat attttaggta
      901 attaacaaga gcaatcagct attcctaaca aaaactacag ttatggctat ataaatagct
      961 aaacataagc taaacacgaa cgttaagtat tattttcgct ttttttgtta aaaaaacgtt
     1021 gaaaagcgaa tgaaacgtct atataatttg aaattgttcg atggatttgc cctattttac
     1081 ttatttttta ttattatagt tttccatcag aaaaaaattc agggagtgga actttttatg
     1141 gaaaagtttt tcggtttaaa gaaaaacaat acaaatgtat caactgaaat aatggcggga
     1201 gtaacgacat tctttgccat gagttatatc ttattcgtaa atccgacgat tttgtcagca
     1261 tcaggtatgc ccttccaagc agtatttcta gcgacaatca ttgcatcgat tattggtacg
     1321 ctggtaatgg gattgttcgc aaatgtacca tatgctcagg cacctggtat gggattgaac
     1381 gcatttttca cttttacggt cgtttttggg cttggttatt tttggcaaca agccttggca
     1441 atggtgttta tttgcggatt aatcaatatt tttattactg tcacaaatat tcgaaaaatg
     1501 attattcggg cgattcccga aagtcttcaa catgcgattg gtggcgggat cggtattttc
     1561 gttgcgtatg tcggaatcaa aaatgcagga ttcttatctt tttcagcaga ccagtcagct
     1621 atttcgagct ctgtcgttga aggcggaaaa gcgacaaatg ttacgataaa tggtggaatc
     1681 gtccctgcgt tagccaactt tgacaacgct cctatcttat tggctgtgat tggtttggta
     1741 ctgacagcca ttttagttgt aaaaaatgtc cgtggggcta ttcttatcgg tatcgtagcg
     1801 accacagtcc ttggtatttt tatgggtgtt gttgatttaa gttccattga ttggcaaacg
     1861 aattcgttag gtaattcatt taaagaatta ggaacaactt tcggtgcagc atttggttct
     1921 gaaggcatgc aatctttatt cagtgattct tccaagattc ctcaagtatt gatgacgatc
     1981 atcgcattca gtttatctga tactttcgat acgatcggaa catttatcgg tacaggacgc
     2041 cgcacaggta ttttctcaaa agaagacgag atcgcgttgg aagatggtcg tgggttcaaa
     2101 acaaaaatgg ataaagcact atttgccgat gcgatcgcta cttctgtggg ggctatcttt
     2161 ggtacatcga atacaacaac ttatgtcgaa tctgcagctg ggatcggggc cggtggacgt
     2221 actggtttga cttcagtagt agtggcagtg ctattcgcat taagtagttt attctctcca
     2281 ctgatcgcca ttgtaccaaa tcaagcaact gcgccagcat tgattttagt tggagtaatg
     2341 atgatggctt cttttgcaga catcaaatgg ctagatatgg aagaagcttt accggccttc
     2401 tttgcatcga tctttatggg attgtgctat agcatttcct atgggattgc agcaggattt
     2461 attttctata caattgtgaa agtaatcaaa ggaaaataca atcaagtttc tgttgctttg
     2521 tggatcgttg atattttatt cattatcaac ttcatcattc tagcaacaat ttaactaaag
     2581 agaagggatt ggaacagtat ttgttctgat ctctttttgt tttacataaa aagaacgtta
     2641 catataaaat gacctttgcg ggatttttct ctttttactg gcgagatcgt tgcttgttta
     2701 ttcgaaaata ggtatgataa aagagacgaa agagggatga tagatggaga ttaattggag
     2761 aaaaaattta gtgattgcgt ggattggctg ttttttcact ggtgcaagta ttagtttagt
     2821 tatgccattc ataccagttt atgttgagca actaggaaca ccaaaagatc aaatcgaatt
     2881 gttttcggga ctagcaatct ctgtgactgc ttttgcatct gccgtggttg caccaatctg
     2941 gggaaatcta gctgatcgaa aaggccgaaa actcatgatg atccgtgcag cagcagggat
     3001 gacattaaca atgggatctt tggcttttgt accaaacgcg tattggcttt tgatcatgcg
     3061 gttttggaat gggattttgt ctggatatat tccaaatgcc acagcgatga ttgcttcaca
     3121 ggcacctaga gaaaagagcg gatgggcgtt aggaacattg tctaccggag cgattgctgg
     3181 aaatctgatc ggaccttcaa tgggcggtgc actggctcag tggtttggta tggaaaatgt
     3241 attcttgatc acaggggctt tgttgatgat cacaacggta ttgaccattt tcttagtcaa
     3301 agaagatttc catccaatcg agaaaaaaga tctgataagc accaaagaaa ttttttcaaa
     3361 aatggatcat ctctctattt tgatcggtct cttcattacg accttgattt tacagatagg
     3421 aatcactacg atcagcccta ttttgacctt atatattcgt gaattgagcg gaagtacaga
     3481 aaatatcctt tttgtcagcg gcttgattgt ttcgatagct ggtgtttccg ctgtcttttc
     3541 ttcaccgaaa ctaggaaaac taggggataa gattgggaat caaaaagttc taattggagg
     3601 actcatttta tcattttgct gttatttacc tatggcattc gtcactacac cgctccaatt
     3661 aggtatactt cgctttattt taggcttttc aactggcgct ttgatgcctt ctgtcaatac
     3721 actgatcagt aagattacgc ctcaagaagg agtcagccga atctatagct acaatcagat
     3781 gtttagcaac tttggacaag ttttaggtcc catgctagga tcgacagtcg cgcatgctta
     3841 taattacagt gctgttttta ttgtgacaag tttgtttgtt ctgtcaaata ttcttctttc
     3901 tttattcaac tttcgtaaag ttcttcgcag cagattataa tggtggtgag gagtagaaaa
     3961 taaaaaagag cttgagaaat ctcaagctct tggctgtttt tttacgaatg ggcagaggtt
     4021 tcttttcttg gcgcaactgc accaatcaaa aggataatga cacccgcaat gaatgaaatg
     4081 attgttgtca aagtaaaatc atatgttccg cttgttaatg cagcaccaat ataaccgact
     4141 acttgaccta gaacgacagc ccagaataat gtaacgatat atcgcataaa gaacacctct
     4201 tttctatctt aattttacca gattttcttt gaatgtaaag aaacgagacc aaatctcaat
     4261 aaaatttgag ttcttgcgaa agaactcatt cctacagatt acaagcaagc aaatgaagtg
     4321 gaataatcaa cttcagcgcc tgctatctta atgaaagtaa aaggatcaaa ccttttaacg
     4381 ctagtgaatt ttaacgaaaa aaaaggcgga atgcaagatg tttttcccac aactttatac
     4441 aattacttct tattctcttt tacaaagtac gatacgtatt cgagaatatg tacaagaagc
     4501 aaaaaaacaa ggctacactt cattagccat aacggataaa gatgtcttat acggtgcacc
     4561 tgaattctat cgtatttgtg tagaagaagg agtacagcca atcattggat tacatcttag
     4621 ttatacttat gaagaaaaaa attatggctt gcttgcgtac gcaaaagatg aaataggtta
     4681 tcagcagctg atgaaacttt ccagccgaaa aatgatccaa gaaaaattgg aacttgcaga
     4741 aatgacagaa accgaccatc tagcgtttgt tgttccggca aatcattctg tttctgaggc
     4801 tttcaataaa gaggataact ttttcggtca ttggcagttt ttacttgaaa atgtttttac
     4861 tagaaatttt tatatcggct tgacagaaaa cacgcctgat caattgcctg aatggtatca
     4921 gtatgtgata aaagaaaatt attcgctttg tgcattacat cccatcgcta gtttacaaca
     4981 agaagaactt tttgcacttg aagtgatgaa acatatcaaa gagggaagcc aaataccttt
     5041 agaaaattta tctgcatctt taccactaac agcaaatgtc tatcttcttt cagaacaaga
     5101 aatgatcaaa cgattcgaaa aagcgggtct ttcccaagct gtaataggcg cccagcgttt
     5161 agctgaatcg atttcatttg aattccagtt ccatcagaaa ttacttccgc attatccagt
     5221 tcctaatgga aaacaagctg gagaatattt aagagagtta tgctattcgc aagtggaacg
     5281 ccgaatcact gactatacaa aggaatacca agaacgtttg gactacgagt tatctgtcat
     5341 ccatcaaatg ggattcgatg attatttctt gatcgtatgg gatgtaatgg cttttgctca
     5401 tagaaacaaa atcgttaccg gtgcaggtcg tggttctgct gcgggttcat tagtagccta
     5461 tgtgttagaa atcacagatg tggatccatt agaatatgat ttgctgttcg aacgcttttt
     5521 aaaccccgaa cgctttacga tgccagatat cgatttggat attcctgaca atcgacgtga
     5581 agaagtattg aattatgttc gagaaaagta tggacaatat catatggcac aaatcgcaac
     5641 atttggcaca atggcagcaa aaatggttct tagagatgca gctagagtgt ttggtttatc
     5701 gcaaagtgaa gcaaaccgct ggtcaaaagc aatacccaat caattgaaaa tcacattgac
     5761 ccaagcttat aaaacttcgc atcaattagt agaattagtt caggcaagcg aaaaaaacca
     5821 acttttattc aaaactgcag tgattttaga aggattgcca agacatgttt cgactcacgc
     5881 agcaggtgta gttatcagcg ataagaatct acttgaacta gttccgctcc aacgcggttc
     5941 tgatgaagct tttctgaccc agtatacgat gaatgaggta gaagcaatcg gtcttttgaa
     6001 aatggacttt ttaggactac gcaacttatc gattattgat tttactttga aagctgttaa
     6061 gcgagtagaa gggtatgaga tccgattaaa agagatcccg ctaaatgatg caaaaacgct
     6121 gctcttgttt caacgaggcg aaacctcagg agtatttcaa tttgaatctg ctggtatccg
     6181 gaacgtttta cgaagattag atccagaaaa catcgaagat attgccgcag tcaatgcatt
     6241 gtatcgacca ggccctatgc aaaatataga taccttcatt gccaggaaga aaggaaaaga
     6301 agccattcgc tacccagatg attcactgat cccgattttg aaaaacacat atggagtgat
     6361 cgtctatcag gaacaaatca tgcagatcgc ctcgactatg gctggattta cattagggca
     6421 gtcagatatt ctgcgacggg cagtgagtaa aaagaaaaaa gacgtattag atgaagaaaa
     6481 gtcgcatttt gtatcaggag cattacaaca gggatattcg gaaaaaacag cagcgaccgt
     6541 atatgaatat atcgaaaaat ttgcaaacta tggattcaat cggtcacatg catttgctta
     6601 ttcattcgtg ggattccaaa tggcgtatct aaaagcccat catccaggtg ctttctttac
     6661 cagcctgatg aattctgttc ggcataacac atcaaaattg aaagaatata ttgcagaagc
     6721 acgtaaaaat aaactcaaac tttcctcgcc gtccattaat caaagtaatt ccagttttga
     6781 gttggtcaat aaggaagaaa tcagattcgg actgacagcg ataaaaggta ttcggcgtga
     6841 ttttgtagaa gatatattaa acgaaagaaa acaaaatggc ccgttccagt cagtggatca
     6901 attcctcata cggatcgata agcgttggct gaaattagag ttactgcaat cactgacagc
     6961 agtaggcgtt tttgatgaat tatctcctaa caggaaacaa ttgatgcttg atctcgaagg
     7021 aaaaatccaa aatatcgttt acagtggtgg aagtcttgac ttgctgggaa tcatggcatt
     7081 aaaagaagaa gaagtcgctg actatacact agaagaacgc ttgaagctgg aagaggagta
     7141 cttaggtatt tatttgtcag gtcatcctgc agaagggttt gatcgtcttc gtttagctaa
     7201 acaagttcag ctgattacag atatcgtccc taatcaacca gtcaatttat tagtgatgat
     7261 caaggaagtt agagaaattc ggacaaaaaa aggagaactg atggcctttg ttgaaggaac
     7321 cgacagtagt ggagaaattt ctgtcacagt ttttccaact ctttatcgta aatatcgtaa
     7381 tttgtttgcc gttgatcgag tgatctttgt tcaagggcgg agcgaaatca gcaaatataa
     7441 tcaggaactg caactaattg gagaaagctt tgctgatcct aaccagttag aagcccagta
     7501 tccttcacaa acttgctatt tgcgtattac agaagaacgg gaagatcccg agataatgaa
     7561 acaattacaa gtgatttttc agaaacaccc aggatatata ccagtagtta tgtatcacga
     7621 aaaaacgcaa cgaaaagtag ttctgtcaga tgcttattgg gtggatgaca gcaagagtat
     7681 aaaggggcaa cttatttatc tgcttggcga agataatgtg gttttcaaat aacttacggt
     7741 aaaaaatact acttgctttc attttttttc atttttttat ccgtaaatca agacaagtga
     7801 gaaaaataat gttagaatgt tagtcgtggt taacgcaaaa atggttgtaa acctaagtca
     7861 tacttaggat aaagtatttt aatgaggtga atgtttatta tgaaacgcat cggaattttg
     7921 accagtggcg gagacgcacc tggaatgaat gcagctgttc gtgccgttgt tcgtaaaggt
     7981 atttacgaag gactggaagt atacgggatc aattatggtt ttgccggttt agttgctgga
     8041 gatattcgtc gtcttgatgt ggcagatgta ggtgacaaga tccaacgtgg tggtacattc
     8101 ttgtattctg cacgttatcc agaatttgca actgaagaag gtcaattaaa aggaatcgaa
     8161 caattgaaaa aattcggaat cgaaggtcta gtggttatcg gaggagacgg ctcttatcat
     8221 ggcgcaatgg ctttgacaaa acgcggtttc ccagcagtag gtattcctgg aacaatcgat
     8281 aatgatattc ctgggactga tttcacaatc ggttttgata cagcaatcaa tacagtacta
     8341 gaatcaattg accgtattcg tgacacagcg acttctcatg ttcgtacatt cgttatcgaa
     8401 gtaatgggac gcaatgcagg agacatcgct ttatggtcag gtgtggctgg cggagcggat
     8461 gaaatcatca tcccagaaca tgattttgat atggcttcag ttgctaaaaa aatccaagaa
     8521 ggccgcgatc gcgggaagaa acattgcttg atcatcttgg ctgaaggtgt aatgggcgga
     8581 aatgaattcg ctgagaaact ttcagaattt ggcgactatc atacacgtgt atctatcttg
     8641 ggacacgttg tccgtggtgg agcaccaagc gcacgcgacc gcgtgttggc aagtaaattc
     8701 ggtgcttacg ctgtcgaatt gcttcaagaa ggaaaaggtg gattatgtgt cggtatccat
     8761 gacaacgaaa tcgttgcgtc tgacatcatt gatacacttg aaaacaacaa acataaacca
     8821 gacttatcac tatatgatct aaataattca ctttcatttt aagagtgaaa gaaaccaagc
     8881 tatttaagct gtgtacagct ttaaataaac aaaattgaat gggagagttt tttaaatgaa
     8941 aaaaaccaaa atcgttagta cattaggacc agcaagtaac tcagtagaaa ttatttctca
     9001 attgattgaa tctggggcaa atgtcttccg ctttaacttt tctcatggtg accatgaaga
     9061 acaattatca cgtatgacta tggtccgtga agcagttaaa aagacaggaa aagatgtcgg
     9121 tattctttta gatacaaaag gagctgaaat ccgtacaact gttcaaggca caacagaagc
     9181 tgattttggt cgtgcaggat acatcaaatt tgaagtagga gatcaaacac gcatctctat
     9241 ggatcctgaa catgtaggtt caaaagaaaa aatcgcggtt acttacccag gattatttga
     9301 cgatgttcac gttggcggac acattttatt tgatgatggt ttgatcgata tgcaaatcat
     9361 tgaaaaagac gaagcaaacc gtgaattagt cgtagaagtt aaaaatgcag gtatgctagg
     9421 atcacgtaaa ggggtaaatg caccaggcgt ttctattagt ttgccaggta ttactccaaa
     9481 agatgctgac gatattcgtt ttggtttaga caacgatatc gactttatcg ctgcaagttt
     9541 tgtacgtaaa gcacaagacg ttctagatat ccgcgaaatc ttagaagaaa aagacatgac
     9601 tcatgttcaa atcttcccta aaatcgaatc tcaagaaggt atcgacaaca ttgacgaaat
     9661 catcaaagtt tctgacggtg ttatgattgc ccgcggagat atgggtgttg aaatcccagc
     9721 tgaactagta ccaatggttc aaaaacgcat catcaaaaaa tgtaatgctg ctggtatccc
     9781 agttatcact gctacacaaa tgttagaatc aatgcaagaa aacccacgtc caacacgtgc
     9841 ggaagcttct gacgttgcta acgctgtatt tgatggcaca gatgcaacaa tgctatctgg
     9901 tgaatctgca aacggggact acccagttga agctgtttca acaatggcac gtatcgacca
     9961 agaagctgaa aatgcattat cagaattagg tacattccaa ttgaacgaat tcgataaaac
    10021 agatgtaact gaaacaatcg gtttatctgt agctcgtgct gcaaaaaatc taggcgtgaa
    10081 aacaatcgtt gctgcaactg aatcaggata cactgcaaaa atgatttcaa aatatcgtcc
    10141 agatgcagat atcttggctg ttacttttga cgaacgcaca aaacgtggtt tgatgttaaa
    10201 ctggggcgtt tacccaacag ttgctgaaaa accatcaaca actgatgaaa tgtttgaact
    10261 agcagctaaa aaagctgttg aattaggttt cgcatcagaa ggcgatttaa tcttgatcac
    10321 tgctggtgta ccagttggtg aacgcggaac aactaacgtg atgaaaattc aaatgattgg
    10381 ttctaaatta ttagaagctc aaggcgttgg acaaaaatct gtcgttgcaa atgcagttat
    10441 tgcttcatct gctgaagaag ctgtaaaaaa agcaaaagac ggaatggttc tagttgtacc
    10501 atcaactgac aaagaatata tgcctgcaat cgaaaaagca gctgctgtta tcgttgaaga
    10561 aggcggattg acttctcacg ctgcagttgt aggaattgca caagatattc ctgtaatcgt
    10621 tggtgctaaa ggcgcaacaa caacaattac agaaggtgaa ttgattaccg ttgattcacg
    10681 tcgcggcatc gtttaccgtg gcgaaacaac agctatctaa tttagcttaa taaaaaataa
    10741 aaacagctct tcttttacga agagctgttt ttttgtgctg taacgaattc atggataact
    10801 tggactactt gttcttgaag aggcgtgacg atctgctgtg ttcgttttgc caatgaaatc
    10861 aagaagcgag gctgaacttc atctgaaaga gggaggcaaa ctaaatgatc ttcaggatgg
    10921 acagcaatct ctgccaaaaa accgataccc acattttctc ggaccatact ctttaaaatc
    10981 gtcaagtcat tgctttgata aacaactttt ggatgagtat gtgtttgttt agaaagctta
    11041 gtgaatgctg cggggtggac ataatgttcg ttcaatagta caaactgttc atctgctata
    11101 tctttgaaag agacagtatc ctgcttggaa agaggatgtg taggtggcac gacgatcatg
    11161 aaacgtttgt ccaaaagtaa ctctgtttct aaatattgat ccacgattgg ctgtgttgag
    11221 cctaatacag ctaaatcgat cctcccttgt cggatcatcc cgtataagtc ccgagagcct
    11281 ccatcggtaa tctcaatttg attcatcaat tcatgcttga taaagtaact tgacagttta
    11341 ggaaagtatt cattgccgat aataggaggc atgccaaaac gaagcttttt cttttgcaac
    11401 tgagtgattt cagctcttgc agtagaaatc tcttggagaa tggtttgtgc atggagaaac
    11461 agctgctttc cagaaggcgt gacgattaat tcgtgatgcg atcgatctct tgtaataagt
    11521 acggtgtcaa actctttttc aagacgttgt aatgcatatg taatagtagg ttggctcaca
    11581 cggaaataat tagccacttt cgtaaaattt ttttgtttga cgatttggac atagtattcg
    11641 atatctttcg tgttcatgaa gatcatccgc tcctttaatg gaataatgat tattgtaaat
    11701 gattctgagg atgatagcaa acagaggctt tctaataaag atttttctgt ttttttatga
    11761 actgcaaggt gtcaagaaaa tttcattgac aacatgcttt ttgccctgta taattgcttt
    11821 tgttgcttag gagtgatgaa aaatgaaatg gtctttattg gaattgagaa aataccagga
    11881 gacaccgtta acttttcatg agacgctcga cttgaaagcc gctctcatga agcgagacaa
    11941 tttaatactt gatgttgcgc cagtggaagt cgaaggtttg gtctcagttg ataagaaagg
    12001 ctatatcgtt cattacactg tgcagacgac tttgacagtt ccttcaacac gttcactaga
    12061 acctgttgaa ctgcccatgg atttttctgt agacgaagta ttcatgacag aagaacaatt
    12121 ccaaagtaga gatgaacaaa tgtctgcgga tgagattttg ctaattgaag gtccattatt
    12181 gaatttagca gagtcgattg aagacaacat cttattggcg attccaatgc gggtattgac
    12241 aaaagaagag gaagcagcaa cagaacttcc aaaaggcaat gattgggaag ttgtttccga
    12301 agaagaatac cagcgcagaa aaatggaaga agccgagaat aaaattgatc ctcgtcttgc
    12361 aaaactatct gaactttttg attctaaatc aaaagacgac gataacaagt aagattggaa
    12421 tatattgtgt ctaatttaga tacaagactt tacaaggagg tgtagcaaaa tggcagtacc
    12481 agctagaaga acatcaaaag ctaaaaaagc aaaacgtcgt acacactaca aattgacaat
    12541 caaagggtta aacgaatgtc caaactgtgg tgaaatgaaa aaaagccatc acgtatgtgc
    12601 aaattgtggt tactatgatg gtaaggacgt aatgtcaaaa gaagcttaat acattttaat
    12661 tagaaaatgt aaaacctcaa ggctaaccat ggagccttga ggtttatttt tttgtagtcg
    12721 gtaaataaaa aatatgttgt tctttagttt gcaatgtagc cgtgttaagc tataatgtaa
    12781 gagattaggg agaaatcccg tatgtaggag gagttcatat gtcaaaacaa caaatcggcg
    12841 ttgtcggtat ggccgttatg ggtaaaaatc tagccttgaa catagaaagc cgcggctatt
    12901 cagtggcttt attcaatcgt acaggggcta aaacaaccgc tgtagtagaa gaacacccag
    12961 ataaaaattt caaagcaaca tatacaatcg aagaatttgt agaatctatc gagaaaccac
    13021 gtcggatctt gctaatggtc aaagcaggac cggcaacaga tgcaacgatc caagagttgc
    13081 ttcctcacct tgataaaggc gatattttga ttgacggagg aaatacattc tttaaagata
    13141 cgatgcgtcg taatgaagag ttagccaact ctggcatcaa ctttatcggt acaggcgttt
    13201 ccggtggtga agaaggagct ttaaaaggac cttcaatcat gcctggtgga caaaaagaag
    13261 cctatgcgct agttgcacca atcttagagc aaatttctgc aaaagcagaa gacgaagcac
    13321 catgtgtcac atatattggc ccaaatggag ccggacatta tgtgaaaatg gtacacaacg
    13381 gtattgagta tggagatatg caactgattg ctgaatctta tgacttaatg aagaatattt
    13441 taaacctttc agtagaagaa atggctgata tctttaaaga atggaatcaa ggcgaattag
    13501 acagctattt gatagaaatc actgctgaca ttctgacacg taaagacgat gaaggaacag
    13561 gtaaaccagt agtagatgtg atccttgatg cagctgggaa taaaggaaca ggtaaatgga
    13621 caagccaaag cgcgcttgac ttaggtgtgc cacttccatt gatcactgaa tctgtatttg
    13681 cccgctatat ttctgcttat aaagaagaac gcgtacaagc aagcaagatt ttatctcgta
    13741 cgaacgattt tgagttcacg ggagacaaaa aagaattggt cgaaaaaatc cgagaagcgt
    13801 tgtatttcag taaaatcatg agttatgccc aaggatttgc tcaattacgt gtcgcttcaa
    13861 aagaatttga ttgggacttg ccatttggtg aaatcgcaaa aatctggcgc gctggatgta
    13921 tcatccgagc tcgattcttg caaaaaatta cggatgccta tgacaaaaat cctgagatcg
    13981 aaaatttatt gctagatgat tacttcgttg aaatcacgaa aaaatatcag caatcagtca
    14041 gagacgtcgt tgccttggct gtccaagctg gcgtacctgt accgactttc tcatcagcca
    14101 tcgcttactt cgattcatac agagcagaac gtctgcctgc aaatataatc caagcccaac
    14161 gcgattactt tggcgcacat acctatgaac gtgtcgacaa agaaggaatc ttccattact
    14221 catggtacca tgaagaataa tagtctttcg gctgttagga aatgagaaaa taaaaagagg
    14281 acgaaagagc tgaagtcgat tgttatgagt agacttcagc tcttttttaa aattgctatc
    14341 catgataaaa tgactataga tttttaagca tattatcggt ataatgatag agtttttaaa
    14401 aagccagtag aatagaaagg ataaaataat aaatgagcaa tattctaatc attgaagatg
    14461 aaaagaactt ggcacgattt gtcgagctag agttgaagca tgaagggtat aacgcagaag
    14521 tccactacaa cggccgaact ggtttagatg cggcattaaa taatgagtgg gatgccatat
    14581 tgttggatct gatgctccca gaattgaacg gtttagaagt ctgcagacgt gtacgccaag
    14641 tcaaaaatac accaatcatt atgatgactg caagagattc tgtgatcgat cgtgtatctg
    14701 gacttgacca cggagcagat gactacatcg tcaaaccatt tgctatcgaa gaattattag
    14761 cacgtcttcg tgcattgttg cgtcgaatcg atattgaagg cgataaaaat gtggctaaac
    14821 aaactactat tacttatcgc gatttgacta tcgaaaaaga aaaccgtgtc gtacgtcgcg
    14881 gaaatgaagt aattgaacta acaaaaagag aatacgagtt gctgcttact ttaatggaaa
    14941 acgtgaacgt ggtattagca cgtgacgttt tactaaataa agtgtgggga tacgaaacag
    15001 aagttgaaac aaacgtagtg gacgtatata ttcgttatct tcgcaacaaa attgacgttc
    15061 ccggggagga aagctatatc caaactgtac gcggaacagg ttatgtgatg cgctcgtgat
    15121 aaagccgaaa aaatcggaaa aaagagaact gaaagggccc tccttgacaa tcaagtgggc
    15181 ctttgcgagt tctttcttca tattcgttgt gtttacaatt ttcgctgtca ttacctataa
    15241 atcatctatc aatctgattg tggcaaaaga aagaaacaat gtagagcgta cgatttcaga
    15301 agtagcttct cgtttggcta actcggatga agaactgaca ttagtaaata cgtatcgtaa
    15361 tctgacgaaa gcgacaaatg acggagatga ggtcgtagat catacttcac tggtcgaagg
    15421 aaacttaatg aagatcgatt cctttatagc tgagctaggg cagccatcaa tggatcttta
    15481 tgtttatgat ttggacgaga acttgatttt taaaacccat gaaaacagtc gaaacctgat
    15541 tcagactaca cgtaaagttc caacgatcgt tacactagaa ggaaaaactg gctttttatc
    15601 tatccagcct atatattcta aaggtacaag agaaaagatc ggctatgcgc aaagcttcta
    15661 tgaattatct tctttctatg atattcgaaa taagctatta ttgactctta ttgttttaga
    15721 agttgtttct ttgatcttaa gtagtatttt aggtttcttt ttatcttctt atttcttaaa
    15781 accactcaag gtactcagag atacaatgga tactatacgt aaagatccgc aatcagatat
    15841 ccacatgcct gaaatcgaca caaatgatga attggcagat ttagctgaaa tattcaatga
    15901 gatgcttgac cgtatgcgtt tgtatattga acaacaagag caattcgtgg aagacgtatc
    15961 tcatgagtta aggacacctg ttgcaattat tgaagggcat cttagtctgc taaatcgttg
    16021 ggggaaagat gatcctgaga tattagaaga atcattaagc gccagcatgc aagaaattag
    16081 ccgtatgaaa agcttagttc aagagatgct agacttatca agagcagaac aagtagatgt
    16141 tcattatgcg aatgaaacaa ctaaaggaaa agaagtcacc tatcaagttt ataataattt
    16201 caagatatta tatccagact atgtgatcac tttagatgat gatttgacaa aagaagtgac
    16261 cctgcaaatt tatcgtaatc actttgaaca attaatcatt atcatattgg ataacgcagt
    16321 aaagtattca actacacgaa aagaagtcca tatctctatt tcttccactc tgaatgaatt
    16381 tgagatcgcg attcaggact ttggcgaagg aatacccaaa gaagacctta ataagatctt
    16441 caatcgtttt tatcgagtgg ataaagcaag agctagaacg aagggtggaa atggtctggg
    16501 gttgtctatc gctaaacaac tggttgaaaa ctataaagga cgtatcttag cagaaagtgt
    16561 agtagggcaa gggacaattt ttagaatctt tattcctatc gcaaaggcag aagacaagta
    16621 aagcatgtgc tttacttgtt tttttatata tccaacaaag aataaggtac aaaatacaag
    16681 caagttagaa attcagggat gtcattataa aaaaaactag cgaacaatta aatagaataa
    16741 catccgttaa ttaaacgtaa gtagctttta gatgaacgaa aaaacaatga aatgctgtaa
    16801 tattcgtgtt taaaaataat aaataaaaat agggaatttt ctattgcaat tttataaaga
    16861 gattgctata ttattatttg ttctaaatga ttcctttaag gtgtcactaa aaattataaa
    16921 aaatagggtt gacgcacttt tgatgtcatg ttataataac gaagtcgttt ggatgaggta
    16981 gctaataaag cttgatttta tcgaaaaaaa cttcgaaaaa gttgttgaca aataacaata
    17041 aagatgatat gataatcaag tcgctgctaa tgagtgacaa agacgtttag aaaaaataaa
    17101 caaagaagtt gttgacattc tcttctgaat ctgataagat taaagagttg ttgaaacaaa
    17161 aaga
//
LOCUS       DBNIBO010000050        17136 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.053, whole genome shotgun sequence.
ACCESSION   DBNIBO010000050 DBNIBO010000000
VERSION     DBNIBO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.053"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(151..2058)
                     /locus_tag="AC1Y77_001366"
     CDS             complement(151..2058)
                     /locus_tag="AC1Y77_001366"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIQ1574953.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="AC1Y77_001367"
     CDS             2188..3063
                     /locus_tag="AC1Y77_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HIQ1574954.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="AC1Y77_001368"
     CDS             3143..4117
                     /locus_tag="AC1Y77_001368"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIQ1574955.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4297..5442
                     /locus_tag="AC1Y77_001369"
     CDS             4297..5442
                     /locus_tag="AC1Y77_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIQ1574956.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="AC1Y77_001370"
     CDS             5579..5923
                     /locus_tag="AC1Y77_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HIQ1574957.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="AC1Y77_001371"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="AC1Y77_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HIQ1574958.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="AC1Y77_001372"
     CDS             complement(7618..8217)
                     /locus_tag="AC1Y77_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1574959.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="AC1Y77_001373"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="AC1Y77_001373"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIQ1574960.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="AC1Y77_001374"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="AC1Y77_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HIQ1574961.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="AC1Y77_001375"
     CDS             11423..12541
                     /locus_tag="AC1Y77_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIQ1574962.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="AC1Y77_001376"
     CDS             complement(12601..13893)
                     /locus_tag="AC1Y77_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HIQ1574963.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14043..14603
                     /locus_tag="AC1Y77_001377"
     CDS             14043..14603
                     /locus_tag="AC1Y77_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1574964.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="AC1Y77_001378"
     CDS             14732..15109
                     /locus_tag="AC1Y77_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HIQ1574965.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="AC1Y77_001379"
     CDS             15042..17114
                     /locus_tag="AC1Y77_001379"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIQ1574966.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5473 a         3104 c         3651 g         4908 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagcgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caataataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc acgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcca agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggattg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtga
//
LOCUS       DBNIBO010000051        16196 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.054, whole genome shotgun sequence.
ACCESSION   DBNIBO010000051 DBNIBO010000000
VERSION     DBNIBO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.054"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(20..904)
                     /gene="rsmA"
                     /locus_tag="AC1Y77_001380"
     CDS             complement(20..904)
                     /gene="rsmA"
                     /locus_tag="AC1Y77_001380"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIQ1574967.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="AC1Y77_001381"
     CDS             complement(897..1466)
                     /gene="rnmV"
                     /locus_tag="AC1Y77_001381"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HIQ1574968.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1459..2235)
                     /locus_tag="AC1Y77_001382"
     CDS             complement(1459..2235)
                     /locus_tag="AC1Y77_001382"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIQ1574969.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2390..3712)
                     /locus_tag="AC1Y77_001383"
     CDS             complement(2390..3712)
                     /locus_tag="AC1Y77_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1574970.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3887..4474
                     /locus_tag="AC1Y77_001384"
     CDS             3887..4474
                     /locus_tag="AC1Y77_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574971.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4953..6962)
                     /gene="metG"
                     /locus_tag="AC1Y77_001385"
     CDS             complement(4953..6962)
                     /gene="metG"
                     /locus_tag="AC1Y77_001385"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIQ1574972.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7230..7508
                     /locus_tag="AC1Y77_001386"
     CDS             7230..7508
                     /locus_tag="AC1Y77_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574973.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7605..8426
                     /locus_tag="AC1Y77_001387"
     CDS             7605..8426
                     /locus_tag="AC1Y77_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIQ1574974.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8711..9721)
                     /locus_tag="AC1Y77_001388"
     CDS             complement(8711..9721)
                     /locus_tag="AC1Y77_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HIQ1574975.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9733..10686)
                     /locus_tag="AC1Y77_001389"
     CDS             complement(9733..10686)
                     /locus_tag="AC1Y77_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574976.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10676..11746)
                     /locus_tag="AC1Y77_001390"
     CDS             complement(10676..11746)
                     /locus_tag="AC1Y77_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574977.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11752..12795)
                     /locus_tag="AC1Y77_001391"
     CDS             complement(11752..12795)
                     /locus_tag="AC1Y77_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1574978.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12796..13737)
                     /gene="opp3b"
                     /locus_tag="AC1Y77_001392"
     CDS             complement(12796..13737)
                     /gene="opp3b"
                     /locus_tag="AC1Y77_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIQ1574979.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13821..14168
                     /locus_tag="AC1Y77_001393"
     CDS             13821..14168
                     /locus_tag="AC1Y77_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HIQ1574980.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14238..15899)
                     /locus_tag="AC1Y77_001394"
     CDS             complement(14238..15899)
                     /locus_tag="AC1Y77_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1574981.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            16078..>16196
                     /locus_tag="AC1Y77_001395"
     CDS             16078..>16196
                     /locus_tag="AC1Y77_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIQ1574982.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         4580 a         3329 c         2897 g         5390 t
ORIGIN      
        1 tctgtcacaa cctctttttt tactgcatat tttctgacat cgcattgctt aatgcggcga
       61 attcttgtaa tgacaaagtc tccccacgac gttttggatc gattccagct gtttccaagc
      121 tttttgctaa ccatgctttt gtctggtcat ctttgccata cgaatgctgc aagttattcc
      181 aaagcgtttt tcttctttgc tgaaatgccg cttttgtcaa tcggaaaaat gctttttcgt
      241 ctgtcacttc gactgccggt atatctcttc ttgtcagttt caaaatcgct gaatctacat
      301 ttggctgagg gacaaatact gtttttggta cgatgaatgc taaactggct tccatataat
      361 attgaacagc aatcgataat gaaccataag ctttcgttcc tggttcagca gagatacgat
      421 cagctacttc tttttgcatc atgacgacta gttcatccac aaccaaatct gattcaagaa
      481 aatgcatcat gatcggtgtc gtaatatagt aaggtaaatt cgctacgact tttaacggaa
      541 gctcctcatg aaatgtttct ctaacagctg ttgacaaatc ggtctttaaa atatcttgat
      601 gtacaatagt cacattgtca tagggttgca tcgtatcagc caatacaggg atcaaacgat
      661 catcgatctc aaatgctacg acttgttttg catgcttggc taattgttcc gtcaatgcac
      721 cgattcctgg tccaacttcg atcacgttcg tatgttcatc tatttgcgca gtttctacga
      781 ttttccttaa aatatttggt tcagtcagaa aattttgtcc taaacttttt ttaaatgaaa
      841 aaccatgttt caataaaatc tcttttgttc tagagggtgt cgcaatttca cgatattcac
      901 tcatgttgtt cctccactat tttcattgct tgggcgagtt ctttcggcgt aatacgaaac
      961 atcttcaaac gtttttccaa ttgtttgccg ttcgtgtaac cgatgcgcaa ctcgtcacct
     1021 agtttttctc gtttttcttt tgcttgtgcc ccagctatta aaccatagtc taaaaggtct
     1081 tgtctagtga tttcagaaac agccgtttgg gaaatgtctg gcgtcacgac ctttttcaat
     1141 gcttctaaaa tagcagcatc acttgcatgc tcgacaccaa ggcttccctt tttctttggt
     1201 ttagcttgtg tgcgcggcaa aaaagcatgt tttgcctgag gaacagcttc cataatgatt
     1261 tttcttattt tttctcctga aaaatcagga tcggtaaaaa cgatcactcc acgagtctct
     1321 tgggcatgtt caatttgcat caggatctct tcattgattg cagaccctat tgtttctatc
     1381 gtatctgcat caactacttc ccgcagcctt cttgtatcgt cttttccttc aacgactata
     1441 atctcttgga tcttcatctt atccatgttc gcttagacca aataatcggt gcgcatttgt
     1501 ccatgtttgg gcagccactt cttctgtcgg aagattccga agttcagcaa tcttttctac
     1561 tgtataacga gtatagcctg gttcatttcg ctttcctcta aaaggaactg gtgccagata
     1621 aggggcatct gtttccacaa gtaatcgttc gattggtaca tttgctgcag cctcttggac
     1681 gtctaatgct tttttgaaag tgacgacacc gctaaatgaa atatgcatcc ctaagtcaag
     1741 aaatcgtttt gcccattcat aatcaccact gaaactatgc atgattccac cgatatctcg
     1801 aacatcttct tcttttagga tttgatacgt gtcttcaagt gcttctctag tatgaatact
     1861 gattggtagg ttcatttctt ttgcgatagc aatctgtctg cgaaatactt tatcttggac
     1921 ttctttcggg tcttccatcc aatgataatc caagccaatt tcccctaatg ctacaacctt
     1981 tggtaaagtc aattgttctt gtaatttatt ttcgatttct ttcgtataac tgccagcctc
     2041 tgtcggatgc cagccgataa tactgtatat atttggatag tcatggctta attgcaaaga
     2101 cttttcaatc gttggcgtat cgaagcccac gaccgccatt ttcgttaccc ccatttcttg
     2161 tgctcgctga atcgtttctg gaatatcttc attgaactgt tcagcattta aatgggtatg
     2221 agaatcaaat atcatgttta tctctccttt tcattcttac tttagcacat aagcactact
     2281 tgcagaagta gcaaaaatca aaatagaaga acttgtttta ttgaaataga aaaagcacga
     2341 agatctagag gacctagttc gtactttttc tgtctgttgt tgaaatattt tactcttctg
     2401 agcgtttttt ctttttttga taatagactg ggatacctat caaggtaaca cctataccga
     2461 tcaatgctaa tttcgtttgc gtcatcatcg tcatacctaa aatgaaaata gcaccgagaa
     2521 tcgcaataat cggaacgatt ggataaagtg gtactttata tggacgtggc aattccggcg
     2581 ctttttttct taaaataaag acaccgataa aaatcagtaa actgaatagc cacatcacaa
     2641 aaatcaacat gtctgttaaa aagtcgaaag aaccaaggct catcataata caagctactg
     2701 ctaactggaa aaccgcagga acataaggaa ccgtaaactt ctttgaaagc tttgtcagct
     2761 gtttactgaa aggtagctcg tcttctaaag ccatcgcata aggcacgcgg atcccagtta
     2821 gtgtataacc gttcaatgca ccgtataccg agatcaagat cccaatagtg accagctttc
     2881 caccgattcc tccaaaaatg acgtctgatg cttcagaagc tgcattcaaa ttaccggcaa
     2941 gatgatcaat cggcaaagtt ttcaaaaaaa caaaattgat caatagataa acgactgtta
     3001 caaaactcaa accaagaata attgctttag gcaaatcttt ttccggccgt ttcatttccc
     3061 ctgccattgc accgacacct aaccagccgt cataagcaaa caacgttgct aacaacgcac
     3121 tgctcaatcc ttcgacaaaa gtaacatctt ttccagcttc gattggaaat aactggacag
     3181 ttccttgtcc tggggtcaat aatccccata tgacaatcac agcaattggt attaatttaa
     3241 caatcaacgt cactgattgt acagaagcac cgacttttgt ccctaacaaa ttcaaccctg
     3301 tcacagaaac agcggtgatc actgcaatca acaaaagata atcggtagat aattgtaata
     3361 agttcgtcaa ttgtgtcgca aagataatcg ataatgcagc aatattagca ggaaagtaaa
     3421 tcaagctttg cgcccatcct aataaaaagc tgggcaattt tccatacgca gcttcaatat
     3481 attttacagc tccaccagtt tccgggattg ctgtagctaa ttcagccact gtcaatccag
     3541 cacagatcgt taagaaacca cccaaaagcc aagctagcaa tgtcaaactt gtagactgag
     3601 tactagcagt aacagcagca gttttgaaga agacgcctgc accgatcacg gttcccataa
     3661 cggtagctaa tgcgccaaac agattgattt ctcgttttag tttgcctttc attcccatcc
     3721 tctttcttct ttgaaatttg gtataatttt cagtataact gagaatattt taatttacca
     3781 ttcttttttc tccactttca gtgaaaaact ccctcttaag acggatgtat ttctttttta
     3841 tttttcgtat actaaaccta ccttttccac gaaggaggaa cagtttatgg attttaagcg
     3901 ttcttatccg aaattagcag attatttatc caaccgggtc gatttttccc gtaaagaaca
     3961 acagcagtcc cattggcatt ggttcacatt aattttattt ggattaatga ctgttgcaac
     4021 gttatcacaa aaactaatat tgctgacttt gttcatcgca atcgttgcat tgatcaaagg
     4081 tcctgtgatg atcttgtggg ggatgttgta ttcctttttg gttgggcttt ttccgcctat
     4141 cggtattttg ctttcagcga ttttcttttt attgaatatc ggaacattta caaagaactg
     4201 gcggatgacc ttagtcggga tctattttta tttctatcct tttggagtga tggcacttag
     4261 tgaagtcatg cattgggata atcattggtt tatagctggc tccttgcttt tagggttgat
     4321 cttgttacac gtgatgttga caaagctcta tcaacattat ggtatcggac gtacgatttt
     4381 ctggtatgtc ttttcgatcc catttgcact gcttactgct ctcttaccaa gccgtttgaa
     4441 gacaaaaata aaaggttacc ataaaatcaa gtgataggat ttttacaaaa taaagaaaac
     4501 aatcatcgaa gtcaaagccg atgattgttt ttttatctat attttcaatc acacatataa
     4561 gagcacaaac taattgaaca taaagtgata gaacttataa ttaacacatc aacgctactg
     4621 ttagatataa atatagatgt aataaagtca ttgcattcaa actttatgaa tgtaaggaca
     4681 ataaaaaaga gtaccagatg aatcaattaa aatctgcccg accctttatc attatgcttt
     4741 ttttctgttt ttacgattcc tattagtatt attctaaatt aacaaaaatc agcgaagctc
     4801 gtacttcttt ttctaggatt tattttggat agttctctgt ttctgtatta tatgattggc
     4861 ttcaagtaat aagaaggaat ttacgaaaat tgcttctcaa atttcggaaa aagggggtat
     4921 gacacaacta gtgtcatacc ccctttgaat ttctatgcga tttctgagcc atttggcatg
     4981 gattttggtg cttcgattac ttgtaaagag ccatctggtg cttctgcaga aaggatcatt
     5041 ccttgactga tttgtccacg catctttcgt ggtttcagat ttgctacaat gacgactttt
     5101 ttcccgatca attcgcttgg atcaggataa aattcagcga ttcctgacaa gatttgacgg
     5161 tcttggctgt ctccagcatc tagtcggaat tgcagcaact tatctgcccc ttctactttt
     5221 tgacagttga tgacttctgc tacttttagt tctacttttt caaatacatc aaatttgatt
     5281 tgtttttctt tcgttgaaac aagttctgtt tcttctggat cccatttgac tgtatcttcg
     5341 tttgtttgag taccttcaga cattttttct tgaataaaag caacttcttc ttccatatct
     5401 aaacgtggga agattggtgt accttttgca actacttttg ttcctgaagg aaattcgcca
     5461 aagtgtaatc cttcaagatt cattgtttca gagtccaatc ctagttgatt gaagatcttc
     5521 tctggcgttt ctgtcatgat tggctgaagt aatatcgctg cgatacgcaa agattcagct
     5581 aaatggatca tgacactatt caattcagct ttcttttctt cgtcttttgc taatacccat
     5641 ggttctgttt catcaatgta tttgtttgca cgagaaatca gcacccagat ttcagccaat
     5701 gctgtattga attccatttt ttccattgca tcatggtaac gtccgacaac gttagcagcc
     5761 gtcgtagaca gttcactatc aaatggagtg acaagtgagg catagtcagg aacgagtcca
     5821 tcacaatatt tgttgatcat agcaatcgtt cgattcaata agttccctag atcatttgcc
     5881 agatcataat tgacacggga aacaaaatct tctggcgtaa agacaccatc tgacccaaac
     5941 ggaacagcac gaagcaaata ataacggagt gcatctaaac cataacgttc cacaagcatt
     6001 tcaggataaa cgacatttcc ttttgattta gacatttttc cgtctttcat caatagccag
     6061 ccatgcccaa atactttttt aggtagaggc aaatcaaggg ccatcagcat gattggccaa
     6121 tagatcgtat gaaagcgaac gatttcttta ccgaccatct gaacatctgc aggccagtat
     6181 ttttcgaata gactttcatc ttcggagcca tatcctagtg cagtaatata attggataat
     6241 gcatcgatcc atacatacac cacatgtttc ggatcactct tgactggtac tccccaagta
     6301 aaagttgttc gtgaaactgc aagatcctct aatcctggtt tgataaagtt gttgatcatt
     6361 tcgtttttac gagattctgg ctggatgaat tcgggatgtt cttcatagta ttccaataga
     6421 cggtctgcgt atttgctcat gcggaagaag tatgattctt cttttaccaa ttctacttcg
     6481 tgtccacttg gcgctttacc accgatcacg ttaccttcgt catctttgta cacttctgca
     6541 agttgtgttt ctgtaaagaa ttcttcgtca gaaacagagt accagccttc atattcacct
     6601 aagtaaatat ctccctgttc taataaacga tcaaatattt tttgaacagc tgctttatgg
     6661 taatcatctg tcgtgcgaat gaatttatca taactgatat caagcgtttt ccataatttc
     6721 ttgacatcag ccgccatttt atctacatat tcttgcggtt ttacacctag ttcttctgct
     6781 tttgtttcga ttttttgacc atgttcatcc actccagtga gataaaacac atcaaagccc
     6841 atcaaccgtt tataacgagc aattgcgtca catgcaatcg tagtatacga gttgccgata
     6901 tgaagtttgc cgcttggata atagattggc gtagtgatat aaaaagtttc cttttctgcc
     6961 acggggctct tcctccttta tcgcatgatg cgaattcgtt tccttgctag tataccataa
     7021 aaaaagcatg ctgttagctt tttttcaaaa tgaaaaagac tggataaacg acttctatct
     7081 acgttcaatc tatcctttaa aaggatgaat aggttgaata aatgcgggtt tttgttttgt
     7141 ttcatatcta ctaaaaaacg gctttttatt tctcccctca cacgtggtat aataaagact
     7201 atctatctta gtaagggact ggaacaatca tgcagaagaa acatcttttt tttacactta
     7261 gtattgcttt tctttcatta gcacatttaa tattcagcta tttttatatt cgtatgtatg
     7321 gttattttaa cctccatggg catttgaaca gttttatgac agcagcttgg attttgcgtt
     7381 ttatcattga cgtatatatc gttatttgtg gcttctttgc cattcgtgaa gagcggtata
     7441 aagtattgcc gttttatctg cttttctttt tattcaatct catacttcca tttatttttc
     7501 atatttaaat gataacctag atttttcaag taaatccttg gttttttttc agagtctgct
     7561 acaataaggt aaaaagtact tttactgtca atggaggtgg tcagatgaac cgttttgctt
     7621 ctaaagaaga agaaaaatta tattatgaac ataaagcaag tgaagaagaa tttcttaact
     7681 ggtatcaaaa acaggaacgt ccagaatatg acaagccctc tttgactgtc gatatcgtac
     7741 tgatgtgcta taacaaagaa gaagatcagc tgaaagtttt gttgatccaa agaaaagggc
     7801 atccttatcg aaattcatgg gcattgcctg gtggctttgt ccaaaaggat gagtcaactg
     7861 gagaaagcgt cttaagagaa acaaaggaag aaactggtgt ggttatttca aaagaaaata
     7921 ttgagcaact gcatacattc agtacgccta atcgagatcc gcgaggctgg gtcgtcacag
     7981 tcagctactt agcctttatt ggagaagaac cgctaatcgc cggagatgat gccaaggaag
     8041 tacgctggtt cacactagaa cgtcatagta acaagatcca tttatctagc ggagaagtgg
     8101 ctatctcgct tgatttagtc actggagagt cttctggcaa agatactttg gcttttgacc
     8161 atagtcaaat catcttgaaa gcattcaacc gtgtagtcaa taaaatggaa cacgagccgc
     8221 aagttctaca agttttaggt aaagatttca cgattacgga agctcgaaaa gtatttgcta
     8281 aattcttagg gatcgattat aaaacgatcg atcattccaa ttttaagaaa gctatgctcc
     8341 aatatttcga tgagatcggt gaacgtcctg taggaatcgg ccgtccttca aaaatctatc
     8401 agctcaagcc ggggcaccat gaataaggtt gtgagcaaaa gattcagacc taatcattca
     8461 agaaaaaacg caaaaacaac tctttgtgag ttacgttttt tgtttcagaa ttttgatgta
     8521 ttcgtaaact ttctgaataa aaaattaaca atagcagaaa aagctcgtac gaactttttc
     8581 tgctattgtt ttattggttt gtcataatta gtgagctttt cttcaaaaga aaaattccta
     8641 gaaacaaaag tcattaagac tttttattaa caaggctaac cctaacgctc ctatcagttt
     8701 cattcatttt ttatcccttg ataaccgaat gtctgcccat tttttctact acttggctct
     8761 ttcttacgga attgctttct tctgctccgt cggtattgat caaaagaaat tttttattat
     8821 accattggga cttataacgc aattttgtta ttaatttatg ccagtcgtga atagaaaaat
     8881 cagcatgtgc ctcttctttt ggaaaagatt ctacaaataa gtagtcatat tgtttgacca
     8941 atttcccaga taattgttca agataatctt ctcgctggtt cttttgtttc agataaagat
     9001 ccattacttt tctcttttgt ttctgataat tgcttgcctg atctaacctg atctttctat
     9061 ggtgagcgga gcggactttc acattcaatt tccgctgtgc atgttgcagc ttttcttctg
     9121 tctggtgaaa ttttggtaaa gttacttcta gaggtcgaga tgtttggatc aatgtcttcg
     9181 gatgataggc gatcccgatc catttagcta aagattcttt ttttactggc gaaacatcac
     9241 aaagtagtgc gacataaaat tcttcattat atcgagcaga aatcgtagcg gagcgaatcg
     9301 atccttcaac tggccgatgc tgatttatct tgattagttc tttttgtttt ggtaacttta
     9361 agtagccatt ttccagatag atcgtacggg tttggttgtt ggtcgtataa gattgccaca
     9421 tacttttttt cgttttaagc tttgggaagc tggcacgtcc cttgaaataa ttgcgaaaag
     9481 cccgatccaa attaagctgt gcatttgcta aagcaagact gtctacttct tttaagaatg
     9541 ggtattcttt ttttaatgta gcgggtgtga gcgaaaaatt ggctactcct gtttgttgcc
     9601 aacttttctg ccgctttttc agcaaatggt tataggtaaa tcgagcacat ccaaaagtac
     9661 gaatcaattg ctgtttttgt tcttctgtcg gatacatacg gaatttaaaa gcttttagca
     9721 tctatgtccc tcctagttag caaccagaag agattccttt tgtcgtttta gtttttttgc
     9781 gtaatatggg acttcctgct cagttgcata gacaaaatgt tctggtgcga tttcacgaag
     9841 ttgacgtact tgtccgtcat ctggttcagg ttgatatttg attcttctgc gttttctctc
     9901 atggtctgga tctggtagag gaatagcaga taacaaactt tctgtataag gatgaacacc
     9961 gtgattatag atttcatcac ttgttcccat ctctaataac aagccgttat gcattacacc
    10021 gattcggtca ctaatatgtt taaccatcga caaatcatgt gcaataaata gataggtcaa
    10081 ttgatgttct ttttggagat cttgcaacaa attgaccact tgggcttgga tcgacacatc
    10141 tagtgcagaa attggttcat cacaaatgat aaaacgcggt tttactgcca atgctcgtgc
    10201 aataccaata cgctggcgtt gcccacctga aaattcatga ggataacgag taccgtggct
    10261 tggattcaat ccaacagttc tcaatagatc atcgactttt tttgcacgct cttcagatga
    10321 tttagctagt ccattaacat caattccttc ggcaatgata tctctgactt tcatacgagg
    10381 attcaacgaa gcgtatggat cttgaaagat catctgaaca tcatgccgga atttcgtcaa
    10441 acctttttta tctttgatct tcgttatatc ttgaccgtcg aataatatct caccatcagt
    10501 aggttcattc aagcgaatga tcgtacggcc agtagtggat tttccgcttc cagattctcc
    10561 aactaaaccg aatgtttctc cttcataaat gtgaaagctg atgtcattta cagcatgtac
    10621 ttcattcttt tttcctacat tgaaatactg tttcaatcct ttaacttcaa gtagtttatt
    10681 tggcatcagc aattcctcct tgaccgtaag tatgtttttg tttctcttta aagatagccc
    10741 agcgacgttg gatttcttct ggcggtgtca ctttgggtgc ctgcggagca agtagccaag
    10801 ttgctgcctt gtgtgttttt gatagttcaa aaaatggtgg ttcttgttct gcatcgattt
    10861 tcatcgcgaa ttcatttctt ggatagaatg cgtctccttt tggcgggttc aataaatccg
    10921 gtggagaacc aggaatagca tacaatcgat cgttcgcact atctaatgtc ggcattgaac
    10981 caagcagtcc ccaagtatag ggatgttgcg ggttgtagaa aatttcttcc gatgttccaa
    11041 cttcaacgat tcttcctcca tacatgacag cgacacgatc agcgacattc gctactacac
    11101 caaggtcatg agtgataaag ataattgatg tatcgatttt tgtctgaatt tctttcagca
    11161 gctccaaaat ctgtgcttga atagtcacat caagcgctgt tgtaggttca tcagcaatca
    11221 ggatttccgg ataacatacc agtgcaatcg cgatcacgat ccgttgacgc tgacctcctg
    11281 agaactgatg cggataactg ttgatacgtt tctcagcttc gggtattcca actaatttaa
    11341 gcaattctaa tgcagctttt tgtccctctt tttttgaaac tttattatgc tttctaaggg
    11401 actcagcgac ttgcttccca atgggcatcg tcggatctaa agaggtcatt ggatcttgga
    11461 aaatcatcgc tatttcttta ccacggattt tttgcatctc tctttctgat tttccaacga
    11521 tatcgttacc tttaaagagg atttgaccag catctatatt ggcgttgctt gacagcaagc
    11581 gcatgatgct tctggtcgtc actgattttc cacttccaga ttccccaacg atcgccaatg
    11641 tttctccttt attcaaatga aaattgacac cgcgaatcgc tcgtaccttt ccagcatacg
    11701 tatcaaaaga gatttctaag tctttcactt ctaaaatttt cgtcatcgtc ttcactcttt
    11761 cattttagga tcaaaggcat cacgcaatcc atcagcaagt aagttaaagc caatcatgat
    11821 aaccgataaa gttactgccg gaatccataa tagataaggc agatacagga atgtcttgta
    11881 cccttcgctc aacatcgttc ctaatgaagc tgacggaggc gtaagaccta aaccgataaa
    11941 gcttaaaaag gcttcgaaga aaatagcaga tggaatacta aacatcattt gaataataat
    12001 gacactagag atattcggca aaatatgttt gaatgcaatt ttcattcttg attcacctaa
    12061 tgtcaatcca gctaaaacat attcttgatc ttttaatttc attgtttgtg cacgaacgat
    12121 acgtgccatt ggtatccagt ttgtgatggc catagcagcc acgattgaaa aaattcctgg
    12181 ttcaaaaacg accaacatca aaatcatgac aacgaggttt ggaattcctg ataagatttc
    12241 caaaaaacgt tgcatcacgt tatcgacacg tccgcctaac aaaccagaaa ccaacccgta
    12301 agctacacca atcgttacat ctagtaatgc agcaataaat gcgatcagca acgagattct
    12361 cattcccata aataagcggc ttaatacatc acgtcccaaa ccgtctgttc ctaaaaagaa
    12421 atttacgttt gaagggacgt ttgcttgagc atatttatca acaagttcac caccaaccat
    12481 tgcttttcca ttcaatccat cgatattgat tcccggaata cgaggaggca aattgatata
    12541 agccacgttt tgggcagttg gatcatgagg agaaacaaag attgtgatca cagagatcaa
    12601 aatgatgacc aatagtaaaa ccattgaaat aacggcagct ttgtttttct ttaaacggcg
    12661 ccatgaatct tggataaaat tcaaagaagg tgcagtaatc tgttcacgtt cttcttcggt
    12721 atttttttgt aatggaagaa attcatccgc agggatatct gcaactgttt cataacgctt
    12781 tggtatcatt tccatttatc ctcggctccc ttctgaaaca cgaattcttg gatcaactag
    12841 accgtacaat agatccacga tcaaaataac aaaaatcagc ataaatgagt aaaggatcgt
    12901 tacagccatg atcgtaggat aatcatttgt tgtgattgac ttaacgaatt gttccccgat
    12961 accaggaata gcaaagatat tttctaccac cagtgatccg gtcatcaatg ctacagctaa
    13021 cggtcctaat aaagtcatca atggaatcaa actatttcga agaccatgtt taaaggcgat
    13081 ttcccaacgg ctcaatcctt ttgcttttgc taattccaca taatcactat gcagtacttc
    13141 caccatttcc gtacggataa accgagcaga gtcagcaagc ggcgacatgg ccaacgcaat
    13201 tgttggtaag attgtgtaca caaagccatc ccattttgcg attggcaaaa cttgtaattt
    13261 gattgcaaag atatattgta ataaaacagc aaatacaaag tttggaatgg aacgacctaa
    13321 tatcgctacg agtgtagaag atgtatctgc ccatgtattt tgcttcatgg cagagatcgt
    13381 tcctaaaatc gtccctagga ctgttccaaa gataattgcc tgtaacccta actgcaagga
    13441 tggtccgatt cttccagcta acaaatgtgc gactggctgg tttttaaatt ggaaggaaat
    13501 tccaaaatct ccttgaagca aattagaaag ataaatccca tattgtacga taactggttt
    13561 atccaaaccg acttgtttat ttagcatctc gattgtttca ggactcaaac gttcctgatt
    13621 cgtatatggc gtgcccggca gcaattgcat caagaaaaag gtgattgttg caattaacca
    13681 aagcgtgatg atcatgaaaa agacacgttt taatacatat ttgatataac tattcactaa
    13741 tgtttccccc tagcttttga cttctgacag atgtttcgct acattattag ataatgaacc
    13801 aaaaataaag gagcaatcaa atgaaaaaaa aatctatccc ctacgcagta gcttttctac
    13861 tgattctggt tattttaata aaaaatctca tcaaccattc ctttacactc attcaattat
    13921 ctaatgatct attcttatgg tcattgccgt ttctgattat cggcggtttt ttatgggtct
    13981 tttcttcggg tttcttcgat cattttcagc gatcggtcca tcttgcacga acaaggaacc
    14041 gaaagaaaaa accagaattt tcctcgctct cgtctgccag ctacggtatg tacagctttt
    14101 ggctgatcat tgccggaata ctaattgctc tttctgctat tttcatgctt ttttctttgt
    14161 taggataagt gaatatgaga acgaggttgc gacatttgcc gcagcctcgt tttttctcat
    14221 gctcacggta ttaacgatta ttcttcaacg tatgtccatt tgtaatccca tgaagcgcct
    14281 gccgcatggt gaacgatacc tttgattttt tcattcacta ggtgaccttc tgctttttgg
    14341 tacaccggaa cgacacccat atcatcagag atgatcttga ttgctttttg gaaatcatcc
    14401 caacgggctt gttcatcgcc agcgtctgtt gatgaagctg ctttaactgc ttcgtcatat
    14461 tctgcattgc tccagcgacc acggttgtat gaattacctg tgacaaacaa atcagtaaag
    14521 ctgcttgggt ctgcatagtc agcagcccat ccgcctagta ctgcgtcaaa atcacccgaa
    14581 gttgagcggt caagacgtac agagaatgga acaggtgttg gtttgactgt tacgccatcc
    14641 aagttttctt ggattgagtt ttgaacatat tcgattactt ttttcgatga atcatcatca
    14701 gaagccatca aatcaaattc taaagaatca acgcccaatt cttttttcgc tttttcccaa
    14761 tattcttttg ctttatcttt gtcataagaa actaattttc cagcttcttt tgcaaagtct
    14821 tcgttcgttg ttggactttt taccatatct gatgggatca atcctgtaga gacaactgat
    14881 ccgtcaccta gtacttgttt tactaaagct tcacgatcga tagaataaga aatagctttt
    14941 cttaggttca cattgttgaa tggtgaattc ttttcacgtt gattgaattc gatatagctt
    15001 gtccgcgctt ctttaacaga agtataagct ggatcgtttg cgttttgctg tgctaactca
    15061 ccagacaaga tcacgtcatc tgcttggcca tctttgaata agttcaatgc agtagaagat
    15121 tcttttacta cactgacttt gatttcagaa agttttactg tatctttgtc ccagtaatga
    15181 tcatttttct tataagtcca ttctgtatct gttcctggtc catcaaaatc ttctaatgtg
    15241 aatggtccat tataaacagc tttatcactt gcagatgcat aggcatcccc attttcttcc
    15301 actacggatt gtttttgtgg gaaaaatgat gggaaagcaa gcagatattg gaaatatggt
    15361 gtttgtttcg tcaatttgat ttctaattca tagtcaccaa ctgctttgat ccctagttct
    15421 gatttatctt tctttccagc tgtaatgtct tctgcatttt caactggcgc atacaggtaa
    15481 gcatattcag aagctgtagc tgggtctact gttcgttgcc agccataaac gtaatccgct
    15541 gctgtaacag gatcaccatt tgaccatttt gcgtcttctc gtaatttgat tttgtaagtt
    15601 aatccgtctt cactgacttc tgccaactcg ctagctcctg ccggctgtgg tttgctgtct
    15661 tcatcaagac ggtaaattcc ttcataaacg ttattcaatg ctgtaaaact gattgtatct
    15721 gttgcaactg ataagtcagc tgtaggcatt tcttgaggca cgactaagtt aaatacgcct
    15781 cctccatctg ctgaaccgct ttttgctgaa ctactgcttg tggctgaact gccgccataa
    15841 catccagcaa gtacaaggcc gcataccgca accaaaccaa atgtaaattt ctttttcatt
    15901 ttacgtaccc ctttattgga attttcagaa taattaatat tttctgaagt tgtttaagag
    15961 tttaatgaat aattattaaa aaatcaacac ttttttttgt ctttttcata aaataatgat
    16021 tttttcgtga ggtttcaagg aatttttcag aaaaaatagc tttgtacaag gttttccatg
    16081 gtcttccaaa agccattaaa taattttcgt tatattgtct attattgcca tacaaagata
    16141 aaaaagaata aacaaaaaga aactgtgaca aaagtcttgt cacagtccct tatctg
//
LOCUS       DBNIBO010000052        15969 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.055, whole genome shotgun sequence.
ACCESSION   DBNIBO010000052 DBNIBO010000000
VERSION     DBNIBO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.055"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(65..484)
                     /locus_tag="AC1Y77_001396"
     CDS             complement(65..484)
                     /locus_tag="AC1Y77_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HIQ1574983.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(486..1070)
                     /locus_tag="AC1Y77_001397"
     CDS             complement(486..1070)
                     /locus_tag="AC1Y77_001397"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIQ1574984.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1117..2592)
                     /locus_tag="AC1Y77_001398"
     CDS             complement(1117..2592)
                     /locus_tag="AC1Y77_001398"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIQ1574985.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2773..3084
                     /locus_tag="AC1Y77_001399"
     CDS             2773..3084
                     /locus_tag="AC1Y77_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1574986.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3200..5536)
                     /locus_tag="AC1Y77_001400"
     CDS             complement(3200..5536)
                     /locus_tag="AC1Y77_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1574987.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5678..6346
                     /locus_tag="AC1Y77_001401"
     CDS             5678..6346
                     /locus_tag="AC1Y77_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1574988.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6349..7392
                     /locus_tag="AC1Y77_001402"
     CDS             6349..7392
                     /locus_tag="AC1Y77_001402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIQ1574989.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7629..8510)
                     /locus_tag="AC1Y77_001403"
     CDS             complement(7629..8510)
                     /locus_tag="AC1Y77_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1574990.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8669..9397
                     /locus_tag="AC1Y77_001404"
     CDS             8669..9397
                     /locus_tag="AC1Y77_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIQ1574991.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9553..9870
                     /locus_tag="AC1Y77_001405"
     CDS             9553..9870
                     /locus_tag="AC1Y77_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1574992.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9863..10471
                     /locus_tag="AC1Y77_001406"
     CDS             9863..10471
                     /locus_tag="AC1Y77_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIQ1574993.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10526..11317
                     /locus_tag="AC1Y77_001407"
     CDS             10526..11317
                     /locus_tag="AC1Y77_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIQ1574994.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            11455..11943
                     /locus_tag="AC1Y77_001408"
     CDS             11455..11943
                     /locus_tag="AC1Y77_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HIQ1574995.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            11994..12866
                     /locus_tag="AC1Y77_001409"
     CDS             11994..12866
                     /locus_tag="AC1Y77_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIQ1574996.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13042..13404
                     /locus_tag="AC1Y77_001410"
     CDS             13042..13404
                     /locus_tag="AC1Y77_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1574997.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13413..14165
                     /locus_tag="AC1Y77_001411"
     CDS             13413..14165
                     /locus_tag="AC1Y77_001411"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1574998.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14179..>15969
                     /locus_tag="AC1Y77_001412"
     CDS             14179..>15969
                     /locus_tag="AC1Y77_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIQ1574999.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLK"
BASE COUNT         4962 a         2775 c         3275 g         4957 t
ORIGIN      
        1 aagctatttt tccatttttt tctctcatac tcaggtcata acgctgattt cacaacctct
       61 tctttcataa tggtttttca ccatctttat aataaactgg ttctttgata tggaaatcta
      121 ttttttccaa ccaatctaaa atcttgtaac ctaactcttc tgacatcgga aaagaggcgt
      181 tgatttgtgc attggagata tggtcaacat ggataccgga agtgatcgta caacttcccg
      241 gaacggcagt tttgatgatc acagctattt tttcggctaa gacatcgtct ttatggaacc
      301 gaccatcgtg gctagggaaa cgtacagttt gcatctctgt atcacgagaa aatgtagtga
      361 ctgttccgat atgtggtgta tctccgcctg ttaaagtgat caataagtct ttaccgatta
      421 ctttaacagc agcttggatc gtgaaaccta gtttggttac ttcaactatc gtttcttttt
      481 ccatattaat caccctgcca acgttttgaa tgctcattta caatttcaaa ttgatccaaa
      541 agtttcattg tatggtgatt aacaagatct tggatcgttt gaggctgatt ataaaaagca
      601 ggaattggtg gtatgatttg cacacctagt cgtgctaatt tagtcaagtt ttccaagtga
      661 atggcattta atggcgtttc acgaggaaca atgactaact ttcgttgctc cttcattgtt
      721 acatcagcag cacgtgcaat caggttgtcg cctaaaccaa cagagatacc tgcgactgtt
      781 ttcatacttg ccggtacgat gatcattcca tcggtcaaaa atgatccgct ggcgacagca
      841 gcacctaaat ctttgtttga ataatggtaa tcaaacaggc tttcgaattc agttagtgat
      901 aagtcggatt ccagctttaa gttctgtttt gcccatggac tgatgattcc gtgtgtttct
      961 atcatcggat attcttttag tttcttcact aggttgatgg cataaatggt gcctgaagcg
     1021 ccacttacac caatcacgat ttttttcttc cgttgtgcca attgttccat tttccgcact
     1081 cctttttatg atgtcaaaac agttgcagat cattcattat ttcaaatatt tttcccaatc
     1141 ttctacttct tggaattttg cacgtacaaa atgttctttc atatcgaaag gaactgtacc
     1201 gtcaaagata gtttttgcac ttatcccacg gaaacgaata gagccaggat tatattctgg
     1261 taattctgat gggtccaatg gatggttcct cattcctggg atcacgatga tatctttatc
     1321 tccttggaaa cgagtgttga aagtccaaac tacatcgttc atgtcgaaaa tatcaacatc
     1381 ttcatcgact aaaatgactg tcttcaattc tttgaaggct gataaagcaa gaatagctgc
     1441 ttgacgctga ataccttcat ccgcttcgtt ttctttacgg atttgcatga tggtcatcaa
     1501 tttaccacca cctgcaggag gattgtagac atttactact tttcctggaa tagctttatc
     1561 gaccaaggca agaatacttg cttctgtagg aataccggcc atagatacgt gttcttcact
     1621 gggaccaatc gttgtttgca tgattggatt gtcttttcgg tgagtaactg ctttgacttt
     1681 cactacttgt acagctggat tagcatcccc agtatagcca ggaaattctg gcattgcttt
     1741 tcctgagttc gtgttgatat cttcttgcat tgtttcattt ggcatgattt ctgcttcaat
     1801 gatgaattca gcacgagcaa tacccacttc atcgactgct ataccatcaa cgatttgaat
     1861 aggttcgtta cgcaaagctc ctgcacacca tagttcattg tagccaagtg gagtggtagg
     1921 tggttcgaaa gtcgtgccaa tggcaattgc tggatctaaa ccaatattga tagtgatagg
     1981 cattggttta ttcaaggctt cgaattgttt ttggaagtga ccaatgtggc gtccgccagg
     2041 catgatgtac ataccgatcg tatctttatc ttctagaacc atacgatgga tcgttacatc
     2101 gctcattgtt ttttctggat ctgatcctaa gactacaccc attgtgatgt atggaccagc
     2161 atcttcagga gtatttgtag gtgcaggcaa aatgttgcga atatcgaagt ctgcatcggt
     2221 agctagatga acgacttctt gtgctggagc gtctgctttg tctactttca caggtttgat
     2281 tggattttct acagcgtctt tcaagaaacg accaagtgtt cgataatcat ggtgcaaaat
     2341 ttcacctaca cgtttacgac tagccattgt tccgatgttc acacgtgtgc cggggaaacc
     2401 tttgatattg ttgaaagtca tagctggtcc ttcttgagtc ggacgtttta ctgtaccgcc
     2461 agcaccgata taacggtaaa caccagataa ttctgcttca ggatcgattt caatatctgt
     2521 ttcgtgatat tgacctggaa gtgtttttag ttcttcgatg acttttctta aatcatacgg
     2581 ttgatctgtc atgagagatt cctccttagt tgtctttacc tatctatagt aaaaggtgaa
     2641 aaagaggaaa acaattcaac ttgttcggta aagtattcta caatggaatg gatgcgtttt
     2701 tttgataata gaataaacta tgatatactt atcactaaaa gaaagcgcat acttagtgtt
     2761 ggaggaagga aagtgaaaaa aatggaggag tcatttccta aagcagtaaa agtggaaaat
     2821 atagcgaata ttttgaaagt tacatttgaa aacggagaag taaaatacgt gaaatctcat
     2881 tggacagaag agataaccga tgccttgcaa tttggaaaaa aaggaagagg aaaaagaaaa
     2941 aatcttctcg cactttctcg taatatgtgg attgggactg aagtaacgat tgaagcagat
     3001 ggaacagtat tcataaatgg aaaagacagg tatacgccag aagagttgtg gtacaaagga
     3061 aagaaaagta taccagaatt ataaaaaata attaaaaaaa aacagcgcga aacaaggaac
     3121 atattacttc ctcgtttctt gctgttttct actatttcag cgctcttatg actcaaatct
     3181 aaatgtggtt gtcacaacct tattctgcac gcaaagcgat agcggcgtct tttttcgctg
     3241 ccattcgtgc aggaagatga ccgccaagca tcgtcaatat agtagagacg atcactagaa
     3301 taatcgcatg gacaggattt agttgagcaa cattttttag atccgtcatg ttgtataaga
     3361 tgctattgat agggaacgta gctagccatg caataaacac acctaatatc ccagaagaaa
     3421 tacctaaaat acacgtttct gcgtcaaata cgcgagtgat gtctttttta cgtgcaccta
     3481 aagcttttag tacaccgatt tcttttgtcc gttcaatcac agatgtataa gtaatgatac
     3541 tgatcatgat catacttgtt actaaagaga tccctgcaaa agcaataagg acataagtga
     3601 tagcatccat caatccgcca gtcagttcag tcatcgttcc agctaaatca gtataaatga
     3661 ttttatcttc atctgatttg cctttattgt aatcatctag ataatccaag atcttttctt
     3721 tgtcttcaaa attgttcgga taaatcatga tacttgaagg caagctgtct ccacctaaat
     3781 aactaagcaa tgtttgtttg gcattttcgt caactttttc agtagttaag acatttacac
     3841 cactgtctct ttgagctttg acgatttctg attctttgtt ttcgttgacg atttgagtcg
     3901 ttaattgatc gctataagcg attcctggag ataagagatt cattgtagaa gaagatttca
     3961 cgcgtaggat ccccgagatc ttcaattcat cagaagcatt ctgatacatc gcatcataat
     4021 ctgtattagg gataaaattc ccagttggaa gttttgtata gtaagtattg ttgtttacta
     4081 atttgaatgt tgtaccaacg atatcatcaa aatcaagttt ttcgtcttct ttcacatcaa
     4141 atccaagatt cttcaatgcg ttgatattag tattattatt gccgtctacg atcagcacga
     4201 catctgttgc tgaggctgga taacttccgg caagtaaaga ataattgtct tttaaaaagt
     4261 tttctttgct agtgtcaagc tgtgtaggga aacttgacac tcccacacct gtcattgctg
     4321 acatcgtact agataaagaa agagattccg tatcaggatt ttgattcgag aagctgacag
     4381 gctgtacttt gccatttaca tcacgtaaaa gattgattcc tgtagtacgg gtaaatccga
     4441 tattattact taagttagga tcgatgtctg ttacgtaatc gacgtatgtt tgatcaatct
     4501 tattcgtgtg ctgtgcttta tcttcttcgc tgacttttgc agtaatcgtt ttgctgtctg
     4561 gataatccgc tttgcttgca cccaatccgg catcgtcacg agtctggtta gtcgttacct
     4621 tagaaatagt aatcggaaat ttcgccatcg tttcagcttg cgtgttatcg atttgttttt
     4681 ggaatccaga agaaagggac agaacgatcg cgattccgat gatcccaata ctggaagcaa
     4741 aggaggtcaa gaaagtacgt cctttttttg ttcggatatt gttgaaggaa agctttaacg
     4801 ctgtccaaaa actcatcttt gttctgcgta agttaaattg atcatctttt ggtcgttcga
     4861 tgtgaggatg ggaatctgtt aagatcttac catcagaaaa ttcaatgatt cgatctgcgt
     4921 attgatgggc cagttcagga ttatgtgtga ccatgatcac taatttttct ttcgataact
     4981 cttggatcaa ttccatgatc tggatacttg tttctgtatc taacgctccg gttggttcat
     5041 cgcacaaaag gatatctggg tcattggcta gtgctcgagc gatggccact cgctgcattt
     5101 gtccaccaga taactgattt ggctttttat gcatatgatt tgtcaagccg actcggtgca
     5161 atgcatcttc tgcccgtttt cgtttttcgt ctttagaaac accacttaat gtcatcccaa
     5221 gttcaacgtt ttcaataatg cctagatgac caatcaagtt gtaactttga aaaatgaaac
     5281 cgatagagtt gtttcggtag gcatcccagt cactgtcctt aaagtcttta gtagactttc
     5341 cgttgattac catatcaccg gaatcataat tatctagtcc accaatcaca ttcagcatag
     5401 tcgttttacc agaaccacta ggacctaaaa tcgctacgaa ttctttttgt ctgaaagcga
     5461 cagacacacc atccaatgct ttggtggtgg tttcgccgac tttataatat ttttttatat
     5521 cttttaattg aagcataatc acccttctcc cgtattgaaa tattcggtat actaataagt
     5581 atatcagaac aatgatcgta gtatacgaaa taaatgttaa ctgaagatga actacaagaa
     5641 aaaatctaga aatttttcaa aaaacagagg gagatcaatg aatcgtattt tagtagtaga
     5701 agatgatccg caattacagt tgctttaccg ttctgtgttg gaacgagcgg gcttttcagt
     5761 catcaccgca ttgaatggag aggatgcgct gaaaaaacta gaagcgacac gtatagaatt
     5821 aatcattaca gatatcatga tgccgaaaat ggatggctat gacttgctag actcattacg
     5881 tagcaccaga tttgacacac ctgttctaat gattacggca aaggccgatt ttgaggataa
     5941 gaaacgcggg ttcaaattag gagcagatga ttatatgaca aaacccatcg atgtcaatga
     6001 gatgatccta cgtgtggaag cattgcttcg acgttcaaag atcaattaca gcagtagctt
     6061 gataatcgga caaacaaaat tagagctaga tgcttataca gtgttacaag atcagcattc
     6121 gttgattctg cctcaaaaag aatttctgct tctgtataaa ttactgtctt atcccaacaa
     6181 gatcttcact cgccaacagt tgatggatga tatttgggga ttagatacga atacagaaga
     6241 acgaacgatc gatgtacata tcaaacgttt aaggacccgt tttgaaaaga cggaagattt
     6301 tcaaatcatc acagtacggg gactaggcta taaggcggtt atttaaagat gaaaaaaaag
     6361 ctattttcac aattatggat ctattttgct agtattgtct tcatttcgat cttagtgaca
     6421 ctgctgtgtt ttctcggttt tattttctta ttgtctcgac attctatctc gcctgctgaa
     6481 tcggctggaa aaccaacgtt atacccgtta ttcgtctttg cggggttcag tatggtcgtg
     6541 ggtacaggaa tatcgatttt tgttgggcgg cgtatcctgc atccaatcag tgcgctaggg
     6601 acaaatatga gtctggtagc aacgggagat ttttcgattc gaatggatga acagcagaaa
     6661 gtagcagaag tacagcaact gtataaagat ttcaacgtta tggtccaaga actcaatagt
     6721 atcgagacac tgaggaatga ctttgtgtcc agtgtatccc atgaattcaa aacaccacta
     6781 gcaacaatcc aaggatatgt tcaattgtta caagccccaa atctctctga cgaagaacgc
     6841 caaatatttt tatatcggat tatcgaaagt atcacgcagc tttcacaatt aacagagaac
     6901 atcttaaaat tgaacaaact ggaaaaccag cgtatccaac tggaaaagaa agagtatcga
     6961 ttagatgaac aaatcagaga agtaatcgtt ttttttcagc ctaaatggga gaaagaacag
     7021 ctagaattgg atatcgaact tgctgcagta aattacacag gcaatgaaga gtttttgtac
     7081 caagtgtggc tgaatatcat ggataacgcc ataaagtaca atcaaatcaa tggacagatt
     7141 catatcaaac tatttgagac agccacagag attgtactgg aagtgaccga ttcaggtgta
     7201 ggaatgaatg aagagaccag agatcgaatg tttgaaaaat tttatcaagg agatacaagt
     7261 cgtcaaattt ctgggaatgg gctaggactt tcactggtta aaaaaattct agaactgcat
     7321 gatggacgta ttgattatag cagtatcgaa ggagtaggaa cgacagcaat gatacggtta
     7381 agtaaacaat aggaaaaaga ataaaacccc taaaaattct attgaaggtt tttaggggtt
     7441 tttagtcgtt tttttgtgat tagggaaatg ttcttacggt tcttttggca taaaaatcgc
     7501 tgctttctta ttgaactacg agaaatgtta gaggtgtcat tgaaacaagg acatgatgca
     7561 agaaagagag cgaaagtcat gcgtcgctct ctttttttgg gtggatagat ggacagaaaa
     7621 tgtagggctt agttttcttc ttgatttaaa tactcccaaa atgtatctga agccaaagaa
     7681 tgctcaccga ctttacgagt aaatgcaaga taactctctg ttgttggaga aatagggaga
     7741 atcacagtat tttcttttaa tagattcttg actgtttttt ccataacaat agcaatcccc
     7801 atgtcatttg caatcatatt gatgatcaag tcgattcttg ctcctttata agagattttt
     7861 ggttcaaatc cagcttcgcg acacagatct agtacaggtt gatacaagtt cgtagtgggg
     7921 ccaaggagca aaaagttctc ctttttcagt tcgcttagat ccaatttctt tttactagct
     7981 aaaggatgat ttttaggtag aactgctaca aaatcgtctg tttcagtagg caaccactca
     8041 atcgaacttg gtggggcctg gaaacttcta gcaaagtgga tcgtgtcact atgttcattg
     8101 gcgtcattgt aagttaattc gacactttcc acttctttca actggactgt aaattcagga
     8161 tggcgtttca aaaactcggt gattttggaa aaacctttat aattgatcat tgtcggtatc
     8221 gttaagatat ttaatgaaag attgttgtct ttcgtatagg tttcaagcac ttcttgcata
     8281 gcatggtatt gttctacgac attttttaca tatgggagca ataagcttcc agcttcggtc
     8341 aattcgattt ttctatgggc gcgttcgaat aatttgacgt ctaattctgt ttctaacgaa
     8401 agaatctgtt tagaaatatt tccttgggtg gtgaattgtc gatcagcagt tttggtgaaa
     8461 ttcattgttt cggctaaatc aagaaaaact tcgaatttat ggatatccat catattcctc
     8521 ctacttattc caataaagaa tactttaaca caaaaaatga attggtgtaa tcttctctgt
     8581 ctgttacaat ctttcttgca gcgaaagcaa taattacaga aatttttctg atttttttcg
     8641 cttcgtagaa aaagagagga gttttttgat gccaactgga gttttgatca atgtaggttc
     8701 cgttttgttt ggcggactaa ttggcggatt agtaggaaat aaattaagtg aacattttaa
     8761 agcacagctg acgatggttt ttggtgtgtg ttcgatgggg atgggaatct attccattgc
     8821 gccgatgaaa aatatgccgg ctgttatttt tgctttagtg ataggaacag cgattggttt
     8881 gattgttcat ttaggtaatg ggataaataa gggggctgcg ctgatgcaag tacctatttc
     8941 taaaatcttc cctagtgaaa agcttggtat gacccacgat gaatttatca gcacattagt
     9001 gacggtcatt gttctatttt gtgcaagtgg tacggggatc tatggaagtt tagattctgg
     9061 gatgacagga gacagtacga ttttgatttc taaatctgtc cttgattttt tcacagcagc
     9121 tatttttgcg tgtaatttag ggtatgtcgt ttctgtgata gcaattcccc aattcattat
     9181 tttttatatt ttgttcttat tggcaaaatt tatttatcca ttgactacac cagacatgat
     9241 cttagatttt aaagcttgcg gcggattctt gatggtcgct accggtttta gaatgatcaa
     9301 cgtgaaaatg ttcccagtag ctgacatgat acctgcaatg atcgttatca tgccactgag
     9361 ctggatgtgg acaaattgga ttatgccact gttataataa aaatcaaaga gaagccattt
     9421 tttgctgact aaaaaggatc aaatctttta gtcagttttt ttgtatttga caaactgatt
     9481 ttgaaactat attatatgtt gtataatata gattgaaata aaatattaca cttgagaata
     9541 gaaggtgacg taatgcacat ccagattcca actgttcttc ttgatggtac tgtgctagca
     9601 gcattaagca aggaagatat gtatgggtat gccttaacaa aaatcgtaca agaaagtctc
     9661 tctgtcagtg aatctacact atatccggtt ttgagaagat tgaaaaaaga aggatatctt
     9721 gagacctatg atgaaccttt tgaaggcaga aaccgtcgat attactcgtt gacaccaatg
     9781 ggaagtcgtc atttagaaga aattattgat gaatggattg tttttcgaga ttctttagac
     9841 cggttattgg aggagagcac aaatgaatga atatttgaat gaagttgtgg atcatcttca
     9901 agaaattcct gaagacgatc gttttgattt gatccagtat tatgaggaat attttttaga
     9961 ttctggaaaa acattagatc agatcattga tgaatatggt acaccaaagc agtttgcctt
    10021 aaaattaaaa atcagttact tttcagaaga agaacaagca gaaaacgagt tagtgaaaaa
    10081 tccaaaaaga caaattcgcc tgatttggtt aatcattatc ggtttatttg cttctcctgt
    10141 cctcattcca ctagcactgg ggtttatatt ggttattggg ggactattgg ttgctttact
    10201 agcgctcatt tttagtatct atgttttatg tatcagtatc ttaggtgttg gcctattcat
    10261 gatcatctca ggaatcagtg tgtttggaca atcgattctg agtggattat tctttatcgg
    10321 tgtcggtctg ttcgccacag gaagcgcagt atttatttcg ccactgttgc tgaaaggaac
    10381 aaaagggctt ttttatgtca tgatgaactt tgttaaatgg atgggacgac gttttattac
    10441 aaaaagagat ctgcattcag cgggcgtttg aaggaggaca cataaattga gattgaaaca
    10501 ttttttgatt accggtattg gtctgatgct tactggtggc ctcctagcag gtgcaactta
    10561 tacattaggc gctcaaaaga gtctgacttg ggataatggt cctcggttga tcgatatggt
    10621 tactgagaca aaaaaaatca aggatgcaaa caaaatcgtc atccatgcag aaaatcaaac
    10681 agtttcggtc atacgcggat tggattttga gataaaaacg acttatgata agacagaaaa
    10741 gccagaaata aaggagaaaa atggcgtgtt gactgtgaat gcagtcaaaa aatcaacagg
    10801 tgcaatcatt agtttagaag acacaagtgc acaaatcact attgttgtcc ctgcagatca
    10861 tgaattatct gaattgacaa tcgaagggaa taatagtgca attgatatca acgaagtatc
    10921 aatcaaaaaa gtgaatacta agacacatga tggctgggta tcgttcaatg aatttacagg
    10981 aggaacaata actgcgaaag atgaattagg ctcacttcga ttagatgagg ccaatgtgga
    11041 taacttgatc gtcacaggtg aaaacagcaa cttccatcta gaagacagtt tgatcagcaa
    11101 agaaggaaaa gtcatcctaa acggtggcaa tttatctttg catgagatgg aaaacagtgg
    11161 ctatactatc caaacgtctg gtgaatctta tattgaaaaa gactaccagc caataacaaa
    11221 tactttgtta gaacaaggac agaaaaagct aaagatcact gctaaagaca catcgatcaa
    11281 tattacgacg gatcaagagg acaatgaaac agattgatcc tgtactcaac tggatatttt
    11341 aagaaaaaac agcaaagatc cttccaatct ttgctgtttt tcatcgctta acgcacataa
    11401 ctatagtaaa ataagaaaga aaatatctaa aaagaaaaca gggtgaaaga gtatatgaag
    11461 gtagaagaag gtcaaagaga aaagttaaaa acagaaatgg aaagattaca tacttatatc
    11521 actcaactaa gccaaacctt ctatgatcca gacaaagaaa aagtgatggt taattacccg
    11581 aacaattcag aaggtcgcca attagagcaa gtgtaccatg aagttttcaa acatttattg
    11641 acggtgaaga aagagctgga ttattattct ttaccgatta tcgatacagg aatattgaag
    11701 tatgatggga agaaggaacg atttatcttt aaatctgttc gggaagattt gcctctttca
    11761 gcagggatgg atctagaggt cttggtagaa gactatttta cagaagaaaa gcactgggtt
    11821 cgaacaaaat tggattattt accgcaggca gccggaggga cacaggcaag cggttggtac
    11881 atcactgaag acaaagagtt agaactagaa ggtgtaatgg cgcgtattcg aaaaaaaaat
    11941 taaaaaagta acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag
    12001 aaaaaagagc attttatatc aatggttatg cagggattgt cggattggtc gtattagctt
    12061 tagttggatt gtttttcttc tatttaggta tgtggcaagc gaaagtttgg gcactttttt
    12121 taagtatttt tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc
    12181 ctaatcaagc aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg
    12241 gctttttcct tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt
    12301 tcaatagttc tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag
    12361 tagtagtctt taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag
    12421 attttgtaga aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg
    12481 atacattcaa tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt
    12541 tgcaaaaaga gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct
    12601 tgaaccattt agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa
    12661 aagccatttt gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg
    12721 ccttagagca aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat
    12781 tgatcaataa tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata
    12841 caggcgacat gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag
    12901 gacggattat tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa
    12961 gaagaatgat agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa
    13021 agtaagagag aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt
    13081 gtggtacttg caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat
    13141 gtacaaatta cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca
    13201 gtcaactata gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac
    13261 ttcaagggac aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac
    13321 aaaaataaag gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc gaaaacagca
    13381 ctggaaaaac taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg
    13441 taacaaaagt atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct
    13501 tcgatgtaga aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaagtcga
    13561 cacttttgaa tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc
    13621 aaaaagatgt gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg
    13681 gctttatttt ccaagatttc aatttattag aaagcttgac agcacaagaa aatatcgctg
    13741 ttcctctttc tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc caaaaaattg
    13801 cagaacgttt atccatctct catattttgg aaagctatcc ttctgagatt tccggtggac
    13861 aaaaacaacg ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg
    13921 atgagcctac aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg
    13981 aattgaatac aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa
    14041 gttattgcca acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg
    14101 gagatcaaag ccgtgattcc ttctatcgag aaatactatc tattcttgga aacttggaac
    14161 agtaggggga gattgactat gctttggaaa ttatcactta ctgggatcaa gagccgttta
    14221 agagactact tggtgttatt ctcaggatta gttatggcat ctgctatttt ttacatgttt
    14281 gaatcaatgg caagcaacga agcttttttg aaaagtaata cgatcgttgc ggctacagtt
    14341 atcattttcc gattcggttc tgttttacta gggatcatca cattcgttta tattttatat
    14401 gcaaattcct ttttgatgac gatgcggcaa aaagactatg caatgtttat gatgctggga
    14461 gcaaaaggca gaaaaatcgc acagatgatt ttttctgaaa cattcttagt agggatcgca
    14521 gctactttag tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca ccgattatta
    14581 gtggatcagt tagatctcaa tatttcgcat tttacacctt ttagtattca cgggttgttg
    14641 atcactgttt tgttttttgc tgttttgttt ttattagctg ctgttgtcaa tgctttttcg
    14701 attgtcaaaa aaccgatttt agcattgatt cgtgcggatc agacaccaac aagaatcaag
    14761 cagcataaat tcttgttttt ccttgaagta gtactgggaa ttgcttttct aggctgcggt
    14821 tactatatga tgaaacacgt ggggacctat caagtctttg gtataggggt agccttagtt
    14881 acgatcgtat taggaactta tttcattttc cattctgtga tcattttctt tttatcatta
    14941 ctaaagaaat cagaacagat ttcattgaaa aaattgaaca atttcacatt gtcacagtta
    15001 aactttcgta ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct gtttgccttg
    15061 gcactgggcg ctttgacggt aggattaggt tttaaaaatg aaattgcaga cttgacagaa
    15121 aacaattctg gatatgatct agtgatgaat aatcctcggg cagaagatca gcaaaaaatc
    15181 aaagaacttt caccaacact ttctgcttct tatactcaaa aagaagatgc gggaacgatt
    15241 tactataatg caagtgaatt tgcggctcaa ccattgatca tgataaaaca tgaagatgga
    15301 acaccgccga agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga actgactgta
    15361 caagaggaac ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat caaggtagtc
    15421 agtcaatcag aatttgatca actgaacttg ccacaatcca ctttacagct agttcaggta
    15481 aaagacttcc aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa taaaatgaac
    15541 aatccatctt tgcaaaaagt agagtacagc aaccagaaat atgaaatgta tgaagtgtat
    15601 aatggtctat tctctggttt tgaatttatg ggattcttcc taggtttagc tttcttgacc
    15661 atgcttgcta gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga cattgctcgt
    15721 tatgaaatgc ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc gatccgtaga
    15781 gaagtaggcg tgttattctt agcaccagga atcttaggtg tagtccatgt cttattcgga
    15841 ttacaaatgt ttggtctatt catgcaaaat ccatacaaag atatttgggt tccgtttacg
    15901 atattcttcg tattgtattt catctactat gtactgacga catggttata tactggaatc
    15961 gtattgaaa
//
LOCUS       DBNIBO010000053        15599 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.056, whole genome shotgun sequence.
ACCESSION   DBNIBO010000053 DBNIBO010000000
VERSION     DBNIBO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.056"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..98)
                     /locus_tag="AC1Y77_001413"
     CDS             complement(<1..98)
                     /locus_tag="AC1Y77_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1575000.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHT"
     gene            complement(403..1635)
                     /locus_tag="AC1Y77_001414"
     CDS             complement(403..1635)
                     /locus_tag="AC1Y77_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIQ1575001.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1892..2461
                     /locus_tag="AC1Y77_001415"
     CDS             1892..2461
                     /locus_tag="AC1Y77_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1575002.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2639..3079)
                     /locus_tag="AC1Y77_001416"
     CDS             complement(2639..3079)
                     /locus_tag="AC1Y77_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIQ1575003.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3237..4001
                     /gene="map"
                     /locus_tag="AC1Y77_001417"
     CDS             3237..4001
                     /gene="map"
                     /locus_tag="AC1Y77_001417"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIQ1575004.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4033..4956
                     /locus_tag="AC1Y77_001418"
     CDS             4033..4956
                     /locus_tag="AC1Y77_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIQ1575005.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVIGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5032..6171
                     /locus_tag="AC1Y77_001419"
     CDS             5032..6171
                     /locus_tag="AC1Y77_001419"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIQ1575006.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFTAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6164..6964
                     /locus_tag="AC1Y77_001420"
     CDS             6164..6964
                     /locus_tag="AC1Y77_001420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1575007.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTKYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6964..7791
                     /locus_tag="AC1Y77_001421"
     CDS             6964..7791
                     /locus_tag="AC1Y77_001421"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1575008.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIVYDNSPQ
                     RQQNEMFEKENIVYHHDPQNPGLAVAYNFALSQAYEGTRCLVTLDQDTRLDDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7796..8503
                     /locus_tag="AC1Y77_001422"
     CDS             7796..8503
                     /locus_tag="AC1Y77_001422"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1575009.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRIHLITALIKRKWFKR"
     gene            8603..9469
                     /gene="rfbA"
                     /locus_tag="AC1Y77_001423"
     CDS             8603..9469
                     /gene="rfbA"
                     /locus_tag="AC1Y77_001423"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIQ1575010.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9483..10055
                     /gene="rfbC"
                     /locus_tag="AC1Y77_001424"
     CDS             9483..10055
                     /gene="rfbC"
                     /locus_tag="AC1Y77_001424"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIQ1575011.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10077..11105
                     /gene="rfbB"
                     /locus_tag="AC1Y77_001425"
     CDS             10077..11105
                     /gene="rfbB"
                     /locus_tag="AC1Y77_001425"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIQ1575012.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11203..12054
                     /gene="rfbD"
                     /locus_tag="AC1Y77_001426"
     CDS             11203..12054
                     /gene="rfbD"
                     /locus_tag="AC1Y77_001426"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIQ1575013.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12089..14122
                     /locus_tag="AC1Y77_001427"
     CDS             12089..14122
                     /locus_tag="AC1Y77_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7657 domain-containing protein"
                     /protein_id="HIQ1575014.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14166..15446
                     /locus_tag="AC1Y77_001428"
     CDS             14166..15446
                     /locus_tag="AC1Y77_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HIQ1575015.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNTLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLENHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4780 a         2687 c         3246 g         4886 t
ORIGIN      
        1 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
       61 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
      121 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
      181 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
      241 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
      301 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
      361 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
      421 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
      481 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
      541 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
      601 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
      661 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
      721 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
      781 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
      841 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
      901 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
      961 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
     1021 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
     1081 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
     1141 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
     1201 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
     1261 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
     1321 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
     1381 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
     1441 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
     1501 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
     1561 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
     1621 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
     1681 ataccattta attatgattc aaacaatgag gcctttaaat aaaaaaaaga ggttattttt
     1741 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1801 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1861 gttataaaaa aattaaggag attaaaaaaa tatggcaaga aaaaagacga ttactagaga
     1921 acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac gatttactgc
     1981 gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg agtttaaaaa
     2041 tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac aatatgaagt
     2101 gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt atatccattt
     2161 tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg gtggaggaaa
     2221 agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg atccagctta
     2281 tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga tcatagctac
     2341 tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac agattgaaaa
     2401 gatgatacaa gacagtatag atgctatctt gaaattcaaa gaaccaatag aaatcgattg
     2461 aatatactca aaagttccac aaatcagtag ataacaagag atgtgtcaaa ataaggaaaa
     2521 gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc atgaatgcaa
     2581 tgaataaaag agccgtacag attgaataaa tctggatggc tcttttattg caagtatatt
     2641 aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct tcttcttcag
     2701 cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt gcaaaagcag
     2761 catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca gatccgcaga
     2821 caccgtaaat ttttcccgat agatccaatt cttgcaagtc ttcataaaag tctacgatct
     2881 catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata tctgcctcct
     2941 cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata tccagatttt
     3001 caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg ctagcatata
     3061 cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca attatgaatt
     3121 ataccaaaac atcattgaaa agaaaataaa acctagagac acctggggaa tgtcatttgg
     3181 ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac gaaaatatga
     3241 ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct ttattagcag
     3301 atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg gatattgagg
     3361 tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt ggctttgaag
     3421 ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga ttccctcgta
     3481 aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtattgat ttgaaaggcg
     3541 gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag atcgaccgat
     3601 tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa gtagggaatc
     3661 ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat ctagcgatcg
     3721 ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca gcagttcctc
     3781 attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc acgatcgaac
     3841 ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg actgcgtata
     3901 caagagacgg cggactttct tgccaatatg aacatacatt ggctattact aaagatggtc
     3961 caagaatttt gacatcacaa ggagaagaag gcacttattg atctagccag tcaataggta
     4021 cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct gatgcgcttt
     4081 attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc tgtcgtcgtt
     4141 gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa tattttaccg
     4201 tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat tcctgagacc
     4261 gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc aggaggcttg
     4321 ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa tgctttgcaa
     4381 attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat cgtccgtttc
     4441 ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac tatcgttatt
     4501 gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc tgtgaacttt
     4561 atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt tgtcatcatg
     4621 ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat cattccgggc
     4681 acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat ctatgctagg
     4741 tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt actcatgctt
     4801 tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc agttgttcaa
     4861 gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa atggttcaat
     4921 aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt attgtaaatt
     4981 tatatcagta atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt
     5041 tcttttagta tggttataag attttttctg acaattttat tatcactgat attaacacct
     5101 gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata
     5161 aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg
     5221 actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct
     5281 tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa
     5341 aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt
     5401 gattccttca cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta
     5461 acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg
     5521 ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc
     5581 ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc
     5641 ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta
     5701 ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt
     5761 tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc
     5821 attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac
     5881 cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct
     5941 ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata
     6001 ttgattattg ccatgttgtt tactgcaatt ttattatttg aattgattgg tctgatcaat
     6061 gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg
     6121 caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa
     6181 ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga
     6241 taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa
     6301 ttcacttccc gagtacaaaa aaagactaac aaagtatagg aaatatccct acatcgatat
     6361 ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag
     6421 cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac
     6481 agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct
     6541 taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt
     6601 tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt
     6661 tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt
     6721 tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat
     6781 gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta
     6841 cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact
     6901 gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt
     6961 ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct
     7021 ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct tattgtttac
     7081 gataatagtc ctcaaaggca gcaaaatgag atgtttgaaa aagaaaatat cgtttaccat
     7141 catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcatat
     7201 gaaggaacac gttgtctcgt cactctggat caggatacga ggctagacga cgattatttt
     7261 gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt
     7321 tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa
     7381 gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc
     7441 tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt
     7501 ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct
     7561 gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac
     7621 cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc
     7681 agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg
     7741 catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg
     7801 ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt tagatagtat
     7861 tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga
     7921 tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg
     7981 gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct
     8041 aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga
     8101 ctattttgag cagaatccgg atcagctagt agtagtttct gatttgatca ttgtggacag
     8161 cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct
     8221 taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa
     8281 acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct
     8341 gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt
     8401 aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtattca
     8461 tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc
     8521 cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa
     8581 tcaaaaagga gaacatatat ccatgaaggg aatcattctt gctgggggca gcggaacacg
     8641 tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat
     8701 gatttattat ccaatgtcta ctctgatgtt ggcaggaatc aaagaaattc tgattatttc
     8761 aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact
     8821 gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg
     8881 agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg
     8941 tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggag cgacagtatt
     9001 tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca
     9061 agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct
     9121 ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg
     9181 agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga
     9241 acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc
     9301 atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga
     9361 aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt
     9421 gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac
     9481 agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg gatgtctttg
     9541 gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat
     9601 tgaaatatga ctttgttcaa gacaatcatt cactatcagc tgaagctggt gtccttcggg
     9661 ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag
     9721 cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg
     9781 gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg
     9841 gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg
     9901 cagaagcaga tgggggcatc gcatttgatg atccagatct agcgattgat tggccaattg
     9961 atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg
    10021 aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga
    10081 aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg
    10141 taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca
    10201 aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag
    10261 atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag
    10321 aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg
    10381 gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta
    10441 ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg
    10501 aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa
    10561 aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca
    10621 tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc
    10681 aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc
    10741 gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca
    10801 gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg
    10861 aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta aacgaccgtg
    10921 cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag ctaggttggc
    10981 agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac
    11041 atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac
    11101 aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc
    11161 ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt attaactgga
    11221 ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa tctgccatat
    11281 gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat ggcttatatt
    11341 acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt agataaagca
    11401 gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag aaatgtagca
    11461 cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta tgttttcgat
    11521 ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat
    11581 ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc
    11641 atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc
    11701 ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg
    11761 acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt
    11821 tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga aatattgaaa
    11881 gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt
    11941 ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt cataccaact
    12001 tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct
    12061 ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg cacgacgaaa
    12121 agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt ttctaaaact
    12181 ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt taaacggaag
    12241 ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa aaactgatgt
    12301 gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga caccttttta
    12361 cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat taaacggaca
    12421 aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg gaaaaccatt
    12481 caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt gggcgatgaa
    12541 attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga agaggaaaaa
    12601 agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc agtggtggtt
    12661 catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg ctttttatca
    12721 ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt tggtggttat
    12781 tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc tggcgtactt
    12841 gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt catgggatct
    12901 gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca tggttcattt
    12961 ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc caggaaaacg
    13021 ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta caaattggaa
    13081 aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt tctatcactt
    13141 ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag atagcgaaca
    13201 aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt ggattaccgt
    13261 tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc cattaagagc
    13321 catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg cttatatttg
    13381 gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa atgcatggtc
    13441 ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt ttgaaaccat
    13501 cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct ggagacgctt
    13561 gttttacctc gcttttgtcg ccttagttat cgtatctggc tttttcgtca atccagtcgt
    13621 agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa aaatcgatca
    13681 acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca ccccaacatt
    13741 gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat ggaagaaaat
    13801 cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa gagcttttat
    13861 cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg tcacgttgaa
    13921 ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac cattaactga
    13981 ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata aagacggtta
    14041 tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac aaactcagga
    14101 aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata ttagtagaaa
    14161 ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg agtgtcggtt
    14221 tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcagggtta ggttttccag
    14281 caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg gctattttgc
    14341 tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt gttgcgcttg
    14401 cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta tttggagact
    14461 tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag tggacaaaat
    14521 gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg ttatatcgtg
    14581 tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc aatacgcttt
    14641 ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt gctttattga
    14701 tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcatcggt gggatagcag
    14761 tcgttgcagc ctttttaggt atttgggtct gccaatctag aggtgcatta gtcgcgttga
    14821 ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt cgtgcgccat
    14881 ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg gcggctgtct
    14941 ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc tatcagacgc
    15001 taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc cagcgaggaa
    15061 accaattttt agaaaaccac aatagttata attctatttt gaatatctat ggattgatag
    15121 gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg ttgaaagcag
    15181 atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg atgcagtcat
    15241 ttatggagga tacattgact tcatacccat gggttccgat tatttatatc ctgttggcta
    15301 tggccgttca tcgatatgat gaaccagaag ttacggagga attctatgtt atggaagagg
    15361 catatgacga tatgacttat gatgatacat cttatgagga acaaccagat agcgctcatt
    15421 cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct gtgacaaccg
    15481 tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata gaaacgttag
    15541 agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg gtaccaaaa
//
LOCUS       DBNIBO010000054        15486 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.057, whole genome shotgun sequence.
ACCESSION   DBNIBO010000054 DBNIBO010000000
VERSION     DBNIBO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.057"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..120
                     /locus_tag="AC1Y77_001429"
     CDS             <1..120
                     /locus_tag="AC1Y77_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1575016.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(444..527)
                     /locus_tag="AC1Y77_001430"
     tRNA            complement(444..527)
                     /locus_tag="AC1Y77_001430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Leu,seq:cag)
     gene            1055..1555
                     /locus_tag="AC1Y77_001431"
     CDS             1055..1555
                     /locus_tag="AC1Y77_001431"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575017.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1745..2089)
                     /locus_tag="AC1Y77_001432"
     CDS             complement(1745..2089)
                     /locus_tag="AC1Y77_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIQ1575018.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2243..3163)
                     /locus_tag="AC1Y77_001433"
     CDS             complement(2243..3163)
                     /locus_tag="AC1Y77_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1575019.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3350..3841
                     /locus_tag="AC1Y77_001434"
     CDS             3350..3841
                     /locus_tag="AC1Y77_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIQ1575020.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3865..6240)
                     /locus_tag="AC1Y77_001435"
     CDS             complement(3865..6240)
                     /locus_tag="AC1Y77_001435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIQ1575021.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6566..7192
                     /locus_tag="AC1Y77_001436"
     CDS             6566..7192
                     /locus_tag="AC1Y77_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1575022.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7215..7919
                     /locus_tag="AC1Y77_001437"
     CDS             7215..7919
                     /locus_tag="AC1Y77_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1575023.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8111..8944
                     /locus_tag="AC1Y77_001438"
     CDS             8111..8944
                     /locus_tag="AC1Y77_001438"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIQ1575024.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9141..11069
                     /locus_tag="AC1Y77_001439"
     CDS             9141..11069
                     /locus_tag="AC1Y77_001439"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1575025.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11207..11662
                     /locus_tag="AC1Y77_001440"
     CDS             11207..11662
                     /locus_tag="AC1Y77_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HIQ1575026.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            11742..11870
                     /locus_tag="AC1Y77_001441"
     CDS             11742..11870
                     /locus_tag="AC1Y77_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575027.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            12132..12401
                     /locus_tag="AC1Y77_001442"
     CDS             12132..12401
                     /locus_tag="AC1Y77_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575028.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12801..13055
                     /gene="rpsT"
                     /locus_tag="AC1Y77_001443"
     CDS             12801..13055
                     /gene="rpsT"
                     /locus_tag="AC1Y77_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIQ1575029.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13144..14190)
                     /gene="holA"
                     /locus_tag="AC1Y77_001444"
     CDS             complement(13144..14190)
                     /gene="holA"
                     /locus_tag="AC1Y77_001444"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIQ1575030.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14228..>15486)
                     /locus_tag="AC1Y77_001445"
     CDS             complement(14228..>15486)
                     /locus_tag="AC1Y77_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIQ1575031.1"
                     /translation="MSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGS
                     LFTLLIAPVFKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIG
                     RLPLVLSGMYIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQ
                     GDSIFLQSPFRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKL
                     ILTHDDTDHVGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGD
                     RISGYYDLYVLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLK
                     ADVVKLGHHGSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWR
                     TDQQGMITYRWHPVFHPHGLVETMID"
BASE COUNT         4777 a         2927 c         2737 g         5045 t
ORIGIN      
        1 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
       61 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      121 gattcattat ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt
      181 ctttcgcatg acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc
      241 agtatttctt gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa
      301 ctgtcccacc ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa
      361 tttttcctat attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct
      421 tctctttata aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc
      481 tcactagcgc ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta
      541 gtttaggatt acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac
      601 gccctccctt atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg
      661 gttggtgaaa aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg
      721 ggattagtaa ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc
      781 tatcttcttt catttggata ttgattattt cttctgttga ttactatcct tctggaattc
      841 tagacgctga agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga
      901 acttcaaata atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga
      961 tatgacacaa taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa
     1021 caaatgaata aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc
     1081 agatttaaag aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat
     1141 caacaatgct tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa
     1201 aacgtcttta ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga
     1261 aagtgatgaa catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt
     1321 aagaaacggc gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata
     1381 tgccacacaa ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc
     1441 acttttgtct gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc
     1501 tgcgatttat cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag
     1561 aaggattcca aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatttaat
     1621 taacttataa tatagcagac aaaattttat aaacaaagaa accttttatc tcttttcttc
     1681 tagaaaggat aaaaggcttt tttgtttttc ctatttgctt ctcatagcta gtcagccaag
     1741 atttctatcg tatccccaac gcaatccgtg cataacggct catcttatct gtcgtccatg
     1801 ctggatacca gactagtttg acttctgctt tcgttacttc tggcacttct tttaatgcgt
     1861 catggataga ttctgttaaa atatctgcta atggacagcc catagttgtt agagtcatct
     1921 taatgactgt ttctccattt tcaggattga attctatatc ataaatcaag cctaaattga
     1981 cgatgtcgat tcctaattca ggatcaatca ccatttccaa ggcagcaaga atacgttctt
     2041 tgattagttc tatttcttgt ccagaccgtt cttggtttgc ttcgctcatc gtacgacact
     2101 ccttaacacg atttttattt atcatactct ttttttagct gcttgtaaat gagaatttag
     2161 aagtaagatt ctcgtttaaa tttataaaaa tgttcttatt agatgtaata gattaaattt
     2221 attctaaaat aaacctgatt cctcattttt tttgtgaacg attttttatt tctggcaata
     2281 cgccagttgt tcgatacatt ttttgttcgt tttcatttgc aaaccggcca aatactgaat
     2341 gagttggtaa aatattgata aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat
     2401 catacatttc gtagcgggtg atgaccatca tgatcatgtt cccttgtttt tttgaatagc
     2461 caccgacaga cggcagtaat gtcattccac gtaccatccg ttttgctagt tcttctgtta
     2521 cctcttctgg cttgacggta acgatcattg ctgttacttt ttgatgactc gtgtggatgg
     2581 catcaattac ttgagtcatt gcataaatgg aaataatcgt atacaaggca ctttcccagt
     2641 taaagaagaa gcctgcgatg actacaatga taccatttag aatcagcatg tagtttccga
     2701 ctgtttttcc agttgtttgt gaaagtacaa gagagatgat atccatccca ccagttgtaa
     2761 agcccatttt taaagataat cctacgccaa gacctaataa aacaccgcct actagggcat
     2821 tcatcaaaat attatcagtg atctgtccga ctggaagcag catcgtcatc accgatacac
     2881 atacaacatt cacaaaacta aggatcgttg ctcgttttcc tactttgaga aaccctaata
     2941 caaacacagg gatattcaaa agaaaaataa atgttcctgt gtctatcttg atatgaaacc
     3001 attcatataa aagcgaggaa atgatttgag cgatcccatt catccctgca gaaaagacat
     3061 tggctggaat caaaaaataa tttaaagcga ccgcagcggt caatccggtt atcaaaataa
     3121 ccactatttt ctttaatctt tcatttttat gatacgcagt cataatttta ctcacaagca
     3181 cctctcccct actttggtat tccaaatgta agaataacag tttatctatt aaaataaaat
     3241 acttcgaact caaatgagaa gaattttagc aaacaggtaa gaatctgccc taatcctatg
     3301 ataagataga tgtatggtaa atatttatat gtatatcagg agggttccta tggcaaaaga
     3361 tgctagcttc gacattgtat cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca
     3421 aacaacaaaa gaattgtcca atcgctttga cttcaaggat tctacagtag aaatcaaatt
     3481 agagaacagc aatctagttg tattgagcga tgatgatttc aaaatcgaac agatcaaaga
     3541 tgtattattt ggtaagctga tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga
     3601 atcagaacac gctctaggtg gcaaagctcg acaaactgcc gatttggtca acggaatcga
     3661 tcgtgaaaac gcgaagaaaa tcactacagc tatcaaaaat gaaaaactga aagtaaaagc
     3721 acaaatccaa gaagatcaga ttagagtgac aggtaagaac cgtgatgatc tacaagctgt
     3781 gattgcttta ttacgtaaac ttgacttacc aatcgagttg caattcacta actatcgtta
     3841 atcaaaaaac gaggctgacg tctgtcattc ctcgtttttt tgatttactg cttcttttga
     3901 taaccaaaaa cgctgtattc cttcttccaa ttccccaaca cttcgtactt ctcttgctgt
     3961 ttgccaatgt tgtgggaata aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa
     4021 tacaacgatt ccttgatccg ccatgccacg aatcactcga ccaccagctt gcaagacttt
     4081 gttcattcca ggaagctgat aagcgtaagc aaatccaagg tgttccttct catcataata
     4141 tgatttgatc agttcctgct catgattcat ctgcggcaaa cctacaccta cgatcatact
     4201 gccgatcaat cggtcccctc tcaaatcgat tccttctgaa aaaatcccgc caagtacgca
     4261 aaatccaatc aatgtttcag cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc
     4321 ttcctcattc atgtccgttt cttggattag cacattgctg tcgggatatt gctgtctgaa
     4381 tgcttccacc gcaagatcta aatattgata agaaggaaag aaaacgaaat aattcccgac
     4441 ctttccatca gcaaaagcat gaatcgtctc tactaatttt gccagactgt gttccctttt
     4501 tcgataagtc gtctctaggt aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg
     4561 actcggcagt ttgtaagcca gacttttttg tccccccagt gtttcctgat aataagagag
     4621 tggtgaaaaa ctagcagaaa aaagaatact gctttttcct ttatccaacg actgctcaag
     4681 aaacaagctt ggatcaatac aaaactcttt tactatcaga tcgcgatacg ttttttcgac
     4741 tgttgtttcg tagtgatcat cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa
     4801 ataataagga agcaattgtt cttgtgatgg gtgctcagga aattctgcca gccattcttt
     4861 aattttttcg cttaattgat aacctgcctt tactaaagat tcagccggtg ctttttgatg
     4921 atgataatcc caatgatctt cttttgctat ttctcggatc gaatcaaatt cttttaatag
     4981 tttgttgaaa cggcgatgaa gttttttgaa ttcctttggt atagcttcct tacttcgttt
     5041 cgttttttca taagatagtt ctgccgaata catctctcgg gaacgattca ccaaattatg
     5101 tgcttcgtca atcaaaaaca gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa
     5161 ataaacagtt ggatcaaata aataattgta atccccaatg attacgtcac accagatact
     5221 gacatctaat gatagttcga aaggacaaag tgtatgcttt cttgcataag tttcgatgac
     5281 ttcacgagtg atctgattct catgattcaa aagatcccat aaaccttcat taatccgatt
     5341 gtaataacca ttggcatatg ggcactgatc tggattacag gtcgtctcat ctaaaaagca
     5401 gatcttgtct ttcgccgtca aagtgacact ttttacttca gctcctgtat cttttaatgc
     5461 tgtcatagca tcttcagcta cttgtctagt tattgtttta gccgttagat aaaagatctt
     5521 ttctccctct tcttccccta cagctttcaa agcggggaat aacgtagaga ttgtttttcc
     5581 tgttccggta ggtgcttcta caaacaattt ctgtttcgta cggatcgttt tataagcagc
     5641 taccgctagt tcacgctgac ctttcctata ctcatcatgg gggaatttca aagccatcaa
     5701 agaggcattg cgtacagtgc gccagttttc ttgaaaaaca agccattctt tataatcttc
     5761 agtcagcttt ttaaaaaaag tttctaactg ctctcttgtc tgatgttcaa cctttctcgt
     5821 gatcacttct tctgccgtct ggaagtaagt caattgcaga tttatttctt gtaaatcatg
     5881 ttcttgacta tagatatagg catacaccct tgcttgatga aagaaaagtt ccacttgttc
     5941 tggttctaaa tcttcaaatc gtggttcgga ggttttgatt tcatctatat aataaatacc
     6001 gtctcttttg aaaataccgt ctgcacggcc ttcaacgatc aggtaatatt catctaattg
     6061 aactgttgtt tttaaaaaga cttctttttc atagttttct cccgcatctt tttggagctt
     6121 gcggtggatt ctcgctcctt ctaatgcagt atggttactt tttttacgat tgtcgatact
     6181 tccacgtcgc aaaataaatt ctactaagtg ccgcacagat atttttgact gtttgatcat
     6241 ttccaccacc tctttctctc taagattacc tgaaaaaacc tagtttagca aaaagagctc
     6301 tctttccaaa cataaaaaaa caccctgctg attaggcagg gatataggat atagaatttg
     6361 gatttttgga tgaatctaat gtaacaagga catattaaaa tgctatgaaa acttactttc
     6421 cttctcttta gaatatccaa atacttttca tatagtctga taattcagat aagtaaagag
     6481 aaataagtac ctgtttttgt ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag
     6541 ttttggctat caaggaggaa tgcacatgca tatcgaaaga ttaaaaacag gaatgattga
     6601 agaaaactgt tatttggtct acaacgaaga agcacttttg atcattgacc ctggagaaga
     6661 tgcggaacga atcaaacaag aaatcaaaaa aacaaaccaa cagccggtag cgattttgct
     6721 tacccatact cactacgatc atatcggtgc agtggaacct ttacgcgcgt attatcaaat
     6781 cccagtttat gtcagcccac tagagcaaaa atggctaggt gatccgattt tgaatctttc
     6841 tggactcggt agacatgatg atattgctga tgttattgtc tcgcctgcag aatatgaatt
     6901 tgaaatgaaa acttatactt taggaaacat gtcttttcgc gtcgtcccta ccccagggca
     6961 ttctatagga agcgtcagct ttatttttga tgattttgtc gtttctggcg acgccctgtt
     7021 tagaggaagt atcggtagaa ccgatctgta tacagggaat ttagaacaat tacttcatag
     7081 tattcggacc cagctgtttg tattacctga tgaatttgca gtctatccag gacatggcga
     7141 tgcaacgacg attgaacagg aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt
     7201 aggaggagaa taaaatgaca gaaactacac tttatatcgt acgacacgga aaaacgatgt
     7261 tcaatacaat tgaacgagta caaggctggt gtgatacacc cctcacaaag caaggacaag
     7321 aaggcattca ttatttaggt cgcgggttaa aaaatgtcga cttttcattt gcttattcta
     7381 gtgacagtgg tcgtgcaatc gaaactgccc gtattattct aggagaacat tctaagggaa
     7441 aagaaattcc ttattttatt gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag
     7501 gtggatatga catggaaatg tggggtgtga tccctcgtgt attgaatttt aaaacatatg
     7561 atgaaatgtt taccacagaa gtttcttttg aacaaattgc taatgctgta tacaacttag
     7621 ataccgctgg gtgggcggaa acttatgaaa cattgcgaga ccgtgtatgg acaggatttg
     7681 aagatatcgc ccatcactgc gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg
     7741 gtttgacgat cgcctttttg cttaatctga tcgatcctaa acagcctgtt cgtgctgggc
     7801 ttttaaatgg cagcgtaaca aaggtcactt atgcaaacgg tcgattttct atccacggca
     7861 tcaatgatac gtcttatatc gataacggga aaacacatag tcgcgactcg tttctataat
     7921 ggggatattt taatttttac cagagaatca taaaaggcaa agtctgaacg agggctttgt
     7981 cttctttttt ctcttcgtgt acttatctgc tttatttctc aagtttttct taatattaaa
     8041 aaacagattg ggtttctcaa aaaataacgg tatactataa gatgtatctt aattgggaag
     8101 gaaggaattt atgttttttg caaatattat caaagcagtg attctcggta ttattgaagg
     8161 aataacggaa tggctgccca tcagtagtac tggtcatttg atcctagcag atgaattcat
     8221 caaactggat gccagttcac aatttatgtc gatgttcaat gtcgttatcc aattaggggc
     8281 gatcttggct gtcgtcgttc tttattttca caagttaaac ccattttctc caagtaaatc
     8341 aacggtagaa aaaaaagata cgtggacact ttggtttaaa gtgatcgttg cttgcatacc
     8401 agccatcatc attggtttgc ctttggatga ttggttagaa gcaaatttcc acaagttctt
     8461 accagttgcc attgttttga tcgtttacgg tattgccttt attattgtcg aaaaacgcaa
     8521 caaaaacaaa acacctcgtt ggacttctct gaatgacttt acgtaccaag cagctcttct
     8581 tgttgggtta ttccaagtct tggcacttgt tcccggaaca tcccgttctg gcgcaacgat
     8641 cctaggatct atcttgattg gtgcttcgcg ttttgtcgct acagagtttt catttttctt
     8701 agggattcct gtcatgtttg gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg
     8761 gaattcattc aaaatggaag aaactgttgt tcttcttgta ggaacattcg tatcttttgt
     8821 ggtttccgtc ttagctatca aattcttact aagctatttg aagaaaaacg actttaccgc
     8881 ttttggctgg tatcgtatta tccttggtat cattttgatt ggttattggt ttattgcaat
     8941 gtagaaaaaa ccagaacact tctcttattt gagaaatgtt ctggtttttt attttcttta
     9001 ataataatca ttataaataa taaaaacaac atcaagctta atcaccaaat gataaccatt
     9061 atcgttctca tttagctgtt attattatca attacatttt tattatgcta tccttgtttt
     9121 gacaaggagg tctaataacg atgactaatc agaaaaaagc ttttgcagca acaatcggct
     9181 gccttttatt tatgatcttc ggttttatct tcgaccgatt ctctcttacc atcaccccac
     9241 ttttttatat cagtgccatt atcatcggtg gatataaaca aacatcggaa ggcttgaaag
     9301 aattgtggaa tgatcggaca ctgaatgtgg atttattgat ggctttagct gctattggtg
     9361 cctgtttgat cggtaattac tttgaaggag caatgttgac attcatcttt tgtttaagcg
     9421 gtgcattaga ggaatacaca actaataaaa gtcaaaaaga aattactgct ttgatgaatc
     9481 ttcaaccaca aaaagcccaa ttattaaaag aaaatggaca aatgcaagaa gtagatgttt
     9541 ctcaactaaa ggtggataac ttggtatttg tcgctaaagg agcagctgtt ccgatagatg
     9601 gctttttaga aagcacacag gcaacgatcg atgagtctgc attaagcggt gaatctgtgc
     9661 ctgtagaaaa atatttaggc gatgagatgt atgcaggaac gctgaatcaa ggaaatccca
     9721 tcacgattcg agtaacaaaa acgagttccg aaactgtctt tgctaagatt atccaattag
     9781 tagaagaagc gcagaatacg ccaactcaaa cagcttcatt tatcgaacga atcgaaaata
     9841 attatgtaaa attgattttg cttgctgttc ctctaatgat ccttctcccc cactttttcc
     9901 taggttggag ttgggatgaa agcttttatc gaggaatggt attattagtt gtggcttctc
     9961 cttgtgcgct tgtcgcatct gctacaccag cttctctagc tgctctttca aattctgcaa
    10021 aaaatgggat tttaatcaaa ggcggtatcc atttagaaaa gctttctgaa ttaaaagcga
    10081 ttgcctttga taaaacagga acgctcacta aaggaaaacc agttgtaact gacagctttt
    10141 tctttgatga aaaggatttg gcacaacaac ttcttgttgc tatggaacaa aaaacgacgc
    10201 atccacttgc tcatgcgatt ttgcaatatt tcgattgcac aatacctgaa gaaatccaat
    10261 acttaccaat agaagaaatt actggttttg gattacaaac tacctattta ggtgcgcaat
    10321 ggaaagttgg caagcacgca tatgatcctg agacgatgat aatcagcaaa gagattgcgg
    10381 aaatgatcga acgtttggaa aatcaaggca aaaccgtgat ttatctttct aaagatcagc
    10441 agttaattgc tgttttaggt ctgctagata ttccaaaagc aaatacccaa caagtaattt
    10501 cctactttaa atcccaaaac atccatacaa gtatgataac cggcgaccat tctggcacag
    10561 caaaggccat tgctgaacaa gtagggattg aacattacta tgcaagctgt actccggaag
    10621 aaaaaacaca actagtcaaa aaagagaatg aactttacca agtaaatgcg atggttggtg
    10681 atggagtcaa tgatgcgcct gctttagcag ccgcttcttt aggtgtcgct atggggcaag
    10741 ggacagatgc cgctatggat atcgcagaca tcgtcttaat gaaaagcgac ttaaataaat
    10801 ttgtctacag tcatcggcta tctttaaaaa tgcggcggat catcaaacaa aatattattt
    10861 tttcgattct tgttattctc ttgttgattg cttctaactt cctgcaattt ttaaatctac
    10921 ctcttggagt tgtaggacac gaaggaagca caatattggt tattctgaat ggcttgcgtt
    10981 tactaagacc tttacctgat ctttctgatt cttcaaaaaa atgtaagtct tgtccactat
    11041 acaaagcgcg gctgtcttct gtcaaataag caaagtgttt ttttatgatt ttgtttattt
    11101 tatgaaaaaa acagcttatg aataggtctt gaaggatgaa ttcctacttt tgtaactttc
    11161 taaaaacatg ttatgattgt ttcgaagaaa gtgaggaata tatttcatgg aaagttggct
    11221 ttttttagga attattttac ttgtagcaat tttagctaaa aatcaaagtc tgatgatagc
    11281 ggcagctttt gttttgatta tgaagctcat accagcctcc cgttcctttt tcccttggct
    11341 tcaaggcaaa ggaatcaata ttggggtgac gatcatcaca gcggctattc taatccctat
    11401 cgcaacagga gaaatcggtc tacgtgatct attagaagcc tttaaatccc ctattggctg
    11461 ggtagctgtt ttttgcggtg ggctagtggc tgtcttatct tccaagggtg taggattgat
    11521 tgcttctgat ccacaaatca ctgttgcact tgttttcggc acgattttag gtgttgtctt
    11581 cttaagagga atcgccgccg gaccggtcat tgcttctggt atcacttact gtatcttaca
    11641 agtatttgct ttaggaaaat agaacaaatt ttgtctattt taggagattc ttaacacccc
    11701 caatttactg atccgtgata tactgacaac gaggtgatac aatgttcgaa aaattaaagt
    11761 atttcatagg aacaatcggc tggtttcttc tagcggtact tttgattctg ggcttagctt
    11821 atctattatt tcctgatttg atacaaaatg tgtttgatac tgctttttga ttgacctatt
    11881 aaaacgatat aggggatgct cctgcgatgc aggggctttt tttaatcaat gttttgtaat
    11941 aatttaggcc gtgccaatga gacttcctat agaattcgat gctctttttt ctttttggct
    12001 cttctgctct tttttgttat actagattac agtggacaaa agaaagaatg atcgcatcca
    12061 cttcaacgaa tatcaaaatg gaagaattag tgcctgtttt caggttactt tgaaaagaaa
    12121 ggaagacgct tgtggaaact tttgaaggtc ttttaattga tacgtacgtt ttacaagaac
    12181 agccaatgat gctgactggg atcaacttgc agcttgagga ccgtgtgatc aatggcctgc
    12241 ttaaagatcg ctatttggca aaatatatct tacgtttgcc cctaaataaa tatgtgctgg
    12301 aagtaagtgg caaatggaac cgtcggaacc agttggtcat aaaaaaaatg ttgataaaaa
    12361 atatcgatga atacattaaa ataatcggta caccagaata gcctttactg atcaaaggat
    12421 actggttata aaataactgc agtttcttaa tagaaaaaca aagaacaaca gggaatcgta
    12481 tcccttttca aaaaaaactt tgcaaacggt tctctttgtt atataataac ttagtaccaa
    12541 acattcccat tcaaactttg tttaaataga taaataaaaa tctagtttgg tttgacgcta
    12601 ttgcctccca caatagcgtc tttttgattt ttttcagctt gtactatccg tttttactga
    12661 aaaaaaatcc taataaaaga ctgttaagga aaaaaacttg tcatgttcat attgcaaagc
    12721 acgctgcctt taggtataat aatgaatgtt gaattatgcg gtaacacgcg taagaactta
    12781 agttatcgga ggtgaaacta atgccaaaca ttgaatctgc aatcaaacgt gttcgtacaa
    12841 gcgaaaacgc taatgtgaaa aattcatctc aaacaagcgc tatgcgtact gcaatcaaaa
    12901 aatttgaaga tgctgtagct tcaggtgctg acaacgtaga tgcgttatat aaagaagctg
    12961 ttaaagctat tgatatggct gaatcaaaag gtctaatcca caaaaacaaa gctaaccgcg
    13021 acaaatctcg tttaagcaaa aaaatcgcta aataaaagaa tcggcttcgg ccgatttttt
    13081 tatttgtcaa aaaaaggaca aaaacaaacg accttcccgc atgctttgcc ctcgtctttc
    13141 ttcttatcga tttcttaaca actgccctgt gcgcaaaacg aatagttgaa aacttaattc
    13201 tttttcgatt tgtcccgttt tgatttgata atccatctca actaattgat caaacaactg
    13261 taccagcaaa tgttcatcga atttttttac ttcctgcatg gcaagtttta cacgatatgg
    13321 atggattttc aatgtttcag caatatttgc ctgctgataa ccaatcttta caagaaattt
    13381 tgtctgtaaa aataaacgta attgagaaag caaaatcgca ttaattttga ttgtttcttc
    13441 cccttgcata actaaatctt cgtataaacg caaagcttgt tccgtcttcc cttttaaaat
    13501 atattgggtc atgtcaaaaa tattatgttc taacgtttta ggaactaatt cttctacttc
    13561 tcttaccgtg atttgctgtt gattttgtcc ataaaggacc aatttctgta actctctcat
    13621 cattttggat aaatctaagt cggtcaacct caagaataaa tcaattgcac ttcgttccat
    13681 tttgattgag gaattctcta aggtattcat cagatattga cgtacttctt tttcattcaa
    13741 ctgttggaca ttgatgataa ttgctgtttt tttcaattgt ttcgttactt ttttccgttc
    13801 atccaacttt tcatatggcg caaagaatac taatacagtc gattctaatg gttctttcaa
    13861 ataagccgtc aataaatcag tattttgttc gattccattg tttacttttt cacctgttaa
    13921 aaaataagga ttctctacga atactaaccg ttgatctccg aaaaacggca acgtctctgc
    13981 ttcagctact acctcatcta aacttgcatt ttccatgtca aaacttaaaa aatttaagtc
    14041 atcttcgccc tctaattgga tttttttgac aatttctgtc cgaacttgat cttggagaaa
    14101 cttttctgtt cctaaaatca agtaacaaga tgcaagtttt tctttacgta ttttttgtaa
    14161 ctctgcttgt atactcaaat tactcaccac tttcaaaaat tatcctacca tgagcagatg
    14221 agaattacta gtctatcatt gtttccacta gcccatgagg atgaaaaact ggatgccagc
    14281 gataggtgat cattccttgt tgatccgttc gccatgtttt tatctgattt tcttctagtg
    14341 tctccaatac ttcgctgtgc gggtgaccat aacgattatt tacgccgcag gatataatcc
    14401 catgttttgc ttcaatatgg gaaagaaaat ctgtgttact tgacgtacgg ctaccgtgat
    14461 gacctaattt gactacatcg gctttcagat taggataaat caaaagcaat tgtttttcca
    14521 ttgcttgatt caaatctcct aaaaataaaa agcgcctatt gttatattcc atccatagac
    14581 caatcgaatc ttcatttctt ccctctccct tttcaaaagg agtcaataca taaagatcat
    14641 aataaccgct gatcctgtcc ccatgtttga tttcaatgat ctcagtacca tttttttgag
    14701 cttcagataa atgcctttgt aatggctcat ttcgtccagc gccccaaccg atataaattt
    14761 ttttgatagt aaaatgctga gataacgtgg gtaattctcc cacatgatcc gtatcgtcgt
    14821 gtgtcaagat gagtttatcg attacactta tcccctgacc ttttagatat ggaacgatat
    14881 ttctttcagc aggaggacgt atcatacctt gcgcccactt ttctctttca aagtgtaact
    14941 gacctccagt atcgatcaaa atcgtttctt ttcgaaaagg actttgcaaa aagatactgt
    15001 ctccttgacc gacatcgata aaagcgatcg ttgaagaaaa gcgtaaaaat ggcaaagtca
    15061 aaaaaataaa agaaaaaaga ccaacataaa acaagtgttt cacaggttgt tctttcaacc
    15121 tatccataca aatcagtaat ccgaaaatat acatcccaga tagcacaagg ggcaaacgtc
    15181 ctataatgag agacgaccct gaagaaaaat cgattattcg ctcaaataag attaatccct
    15241 cttctatcag caaaagtaaa gtttccggca gccagcgatt gaagagtgtg agaaataaca
    15301 caccaggaag aaaaagaaac ttgaataccg gtgcaatcag taatgtaaaa agactaccta
    15361 aaataggcca ctcataaaaa tgccatacaa tcaacggcat cgtgatcata gtaaacagga
    15421 cgtgagaggc cgtttttttc aaccaatctc catcaaaaga aatcaggata aacaaaaaac
    15481 tcatga
//
LOCUS       DBNIBO010000055        15202 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.058, whole genome shotgun sequence.
ACCESSION   DBNIBO010000055 DBNIBO010000000
VERSION     DBNIBO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.058"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            141..1187
                     /gene="gmd"
                     /locus_tag="AC1Y77_001446"
     CDS             141..1187
                     /gene="gmd"
                     /locus_tag="AC1Y77_001446"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIQ1575032.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1195..2280
                     /locus_tag="AC1Y77_001447"
     CDS             1195..2280
                     /locus_tag="AC1Y77_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIQ1575033.1"
                     /translation="MNKHICFYLSSHGFGHIARNLPIIMATLCRCEAKITLVCGANHL
                     DFAKRNLEQELPKELIDRVRYRPINTDIGLINREGTLLVDTGKLTEICEYFLAKVPSL
                     AKTEAEWLKREAVSAVLCDMPIWAMDACDMAGVPFLYVGNFTWTELYREFLPEHIWKE
                     YAKRYGKIQHTMLYALNNEEMLEFLQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVF
                     VALGMSARFKQPIDVSHAPYDFITTLGVPLVGENVTVISNSTPNTQDYIAAADYVITK
                     AGWGTVAECILAQRPMALFARDTVLEDRTTICKLQKLGLAISVTPENLHRVERVIEDL
                     KQLDTSNFDTFYDAASEIADKLISLMQ"
     gene            2317..3261
                     /locus_tag="AC1Y77_001448"
     CDS             2317..3261
                     /locus_tag="AC1Y77_001448"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIQ1575034.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3329..4642
                     /locus_tag="AC1Y77_001449"
     CDS             3329..4642
                     /locus_tag="AC1Y77_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIQ1575035.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4834..5628
                     /locus_tag="AC1Y77_001450"
     CDS             4834..5628
                     /locus_tag="AC1Y77_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIQ1575036.1"
                     /translation="MTLTEVLQEHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPT
                     DEYAIEKENGQLGKTEMWYVLDASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKK
                     NDVFFIEAGTIHAIGAGALIAEIQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVAN
                     LKGLSEPKQPLRVLKYRAGVAAELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVL
                     LCVNGCGTVRFDNESLDIYKGDCLFVPADSTEISIHGSIQFLDVRG"
     gene            5633..7285
                     /locus_tag="AC1Y77_001451"
     CDS             5633..7285
                     /locus_tag="AC1Y77_001451"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIQ1575037.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7309..8175
                     /locus_tag="AC1Y77_001452"
     CDS             7309..8175
                     /locus_tag="AC1Y77_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIQ1575038.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            8364..8489
                     /locus_tag="AC1Y77_001453"
     CDS             8364..8489
                     /locus_tag="AC1Y77_001453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575039.1"
                     /translation="MAGITGVNVIVTYNHHLPIQCLDFSCNYYYIELVIPQKSAS"
     gene            8567..8977
                     /locus_tag="AC1Y77_001454"
     CDS             8567..8977
                     /locus_tag="AC1Y77_001454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575040.1"
                     /translation="MEEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSN
                     LWFRQEISIAEIEDAVLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKN
                     ISYLKVYTATPPKKPTPPRFQSNGWSLFFSHKSA"
     gene            9105..9458
                     /locus_tag="AC1Y77_001455"
     CDS             9105..9458
                     /locus_tag="AC1Y77_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575041.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9926..10402
                     /locus_tag="AC1Y77_001456"
                     /pseudo
     CDS             9926..10402
                     /locus_tag="AC1Y77_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10402..10593
                     /locus_tag="AC1Y77_001457"
                     /pseudo
     CDS             10402..10593
                     /locus_tag="AC1Y77_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10602..10887
                     /locus_tag="AC1Y77_001458"
                     /pseudo
     CDS             10602..10887
                     /locus_tag="AC1Y77_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10901..11267
                     /locus_tag="AC1Y77_001459"
                     /pseudo
     CDS             10901..11267
                     /locus_tag="AC1Y77_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            11439..11879
                     /gene="tnpA"
                     /locus_tag="AC1Y77_001460"
                     /pseudo
     CDS             11439..11879
                     /gene="tnpA"
                     /locus_tag="AC1Y77_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12126..12526
                     /locus_tag="AC1Y77_001461"
                     /pseudo
     CDS             12126..12526
                     /locus_tag="AC1Y77_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761554.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12571..13780)
                     /locus_tag="AC1Y77_001462"
                     /pseudo
     CDS             complement(12571..13780)
                     /locus_tag="AC1Y77_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(13902..14855)
                     /gene="yidC"
                     /locus_tag="AC1Y77_001463"
     CDS             complement(13902..14855)
                     /gene="yidC"
                     /locus_tag="AC1Y77_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIQ1575042.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
BASE COUNT         4407 a         2999 c         3603 g         4193 t
ORIGIN      
        1 cacgaaaaac taatattgtg ctaatgcgtg cgtagattgt tctatgaaat atcgaaaatt
       61 atcgtgtatt cttgaaaagt attagaaatt acgagccatt tatatggcct tatcacttac
      121 ataggaaagg ataatgtatc atgaaaaaag cgctgattac tggtattact ggtcaggacg
      181 gttcctatct ggcagatttc ctgctagaga agggctacga agttcacggt atcactcgcc
      241 gtgcttctat ttccaatacc gctcgcattg accatttgat gggcaagatc acccttcatg
      301 acggtgacct gtccgactca tcttctctga ttcgtatcat cagcattgtt cagcctgatg
      361 aaatctataa cctcgctgca cagagccatg ttcaggtttc ttttgatgtt cctgagtact
      421 ccggcgatgt tgatgctttg ggcgttatgc gtattctgga agcttgccgt attctgggcc
      481 tgacgaagaa gactaagatt tatcaggcat ctacttctga gctgtatggc aaggttgaag
      541 aagttcctca gcgtgaaacc actccgtttc atccttacag cccttatgct gttgcaaagc
      601 agtatggctt ctggatcact aaggaatatc gtgaagctta tggcatgttc gctgtcaacg
      661 gtattctgtt caaccatgaa tccgaacgtc gcggtgagaa tttcgtcacc cgtaagatca
      721 ccctcgctgc cggccgcatt gcagaaggcc tgcaggacca tctggaactg ggcaacatgg
      781 actccctgcg tgactggggc tatgcaaagg actatgttga gtgcatgtgg atgattctcc
      841 agcatgagac tcccgaggac ttcgtgatcg ctaccggcga gcagcacact gttcgtgact
      901 tcaccgagaa ggcattcgca gcaaatggca tgaccattcg ttgggaaggc actggcattg
      961 atgaaaaggg ctacgatgtt gaaaccggca agatgctcgt ttgtgtaaat ccacagtggt
     1021 tccgtccgac tgacgttgac aacctgtggg gcgacccgac taaggcaaag accgttctcg
     1081 gttggaatcc gcagaagacc tcttacgagg aactggttaa catcatggca aagcatgacc
     1141 gtgaattggc aaagcgcgaa gctgcaatga agaacgcagt caagtaagct gacattgaat
     1201 aaacatatct gtttctatct ttccagtcac ggcttcggac atatcgcgcg gaatctgccc
     1261 attattatgg caacactctg ccgatgcgaa gcgaagatta cactggtttg cggcgcaaat
     1321 catctggatt ttgcaaagcg caatttggag caggaactgc ccaaagagct gatagaccgc
     1381 gtgcgctatc gtccaataaa cacagatatc ggtctgatta accgtgaggg caccctactg
     1441 gtggacacgg gtaagctgac cgagatctgt gaatattttt tggcaaaggt tccttctttg
     1501 gcaaagacag aagccgaatg gctcaagcga gaagctgtca gcgcagtgct ttgcgatatg
     1561 ccaatctggg caatggatgc ctgcgatatg gcaggagtac ctttcctgta tgtaggaaac
     1621 ttcacttgga cagagctgta tcgcgaattt ttacccgaac atatttggaa agaatatgcc
     1681 aagcgttacg gtaaaattca gcacaccatg ctttatgcgc taaataacga agaaatgttg
     1741 gaatttctcc aaaacagcga aaaaacaccg acttccgttg tggcgcgtcc gattcataac
     1801 gacgaagtgc aacgcattaa atcacagcat gaaaaaccgc ctgtatttgt cgcactcggt
     1861 atgtccgcgc ggttcaaaca gccgattgat gtcagtcacg cgccgtatga tttcattaca
     1921 acgctaggtg ttccgcttgt gggagaaaac gtaaccgtta tttctaacag cacccccaat
     1981 acacaggact atatagcggc ggcagattac gttattacaa aggctggatg gggaactgtt
     2041 gcggagtgta ttctcgcaca gcgtccgatg gcactgttcg cccgcgatac agttttggaa
     2101 gatagaacaa ctatttgtaa attgcaaaaa ctgggtctgg caatttccgt cacaccggaa
     2161 aacctccacc gcgtagagcg ggtgattgag gacctcaaac agctagacac ctcaaatttt
     2221 gatacatttt atgatgctgc gagcgaaatc gcagacaaat tgatttctct catgcaatga
     2281 gagatgctct aaatttagaa aggagcaagc attattatga tggaaaaaga cgcaaaaatc
     2341 tacgtagccg gtcatcgtgg catggctggt tccgcaatcg tgcgcgagct gaaccgtcag
     2401 gactacaaca atattatcac ccgcacccat aaggagcttg acctgtgccg tcaggacgct
     2461 gttgaagcat ttttcgcaca ggaaaagccg gattatgtat ttctcgctgc tgcaaaggtt
     2521 ggcggtatta tcgcaaacca gaacgctctg gcagacttca tgtatgaaaa catgattctg
     2581 gaaatgaacg tcatcaactc cgcatgggag aatggctgta agaagttaca gttcctcggt
     2641 tcttcctgta tctatccgcg tatggcaccg cagccgatgc cggaaagctg tctgctgaca
     2701 agtgagctgg aaaagaccaa cgaggcttat gcactggcaa agatttctgg cctgaagtac
     2761 tgtgagtttc taaacaagca gtacggtact gactatatct ctgttatgcc tactaacctg
     2821 tatggaccta acgacaacta tcatcctact cacagtcatg tgctccccgc tttgattcgt
     2881 cgcttccatg aggctaagga agcaggtctg cctactgtta cctgctgggg tgatggttct
     2941 cctctgcgcg aattcctata tgttgacgat ctggcaaacc tgtgtgtgtt cctgatgaat
     3001 aactatagcg gtgacgagac tgttaatgca ggtactggca aggaactgtc tatcaaggaa
     3061 ctgaccgaga tggtagcaaa ggttattggt tacgaaggcg agattctgtg ggataccagc
     3121 aagccgaacg gtacacctcg taagctgctg gatgtttcca aggcaaccaa gctgggatgg
     3181 acatataaga ctgagttgga agatggcatc cgtctgtcct acgaggactt cctgaataac
     3241 ccgatgcgtg ctgaacgcta atttaccgag gttgctgtga tttttgagga ttgcagcagc
     3301 ctctttctta taaaaaggag agatcattat gaatattgtt ttactgtctg gcggttctgg
     3361 taagcgcctt tggccgctgt ctaatgacat ccgttccaag cagttcatta agattttcaa
     3421 aactgaagat ggatatgaat ccatggttca gcgtgtctac cgccagatta agaaggtaga
     3481 taacgacgca actgttacga ttgcaacctc taagacacag gtttctgcta tccataacca
     3541 acttggtgag gatgtgggtg tatctgtaga gccttgtcgc cgcgatacct tcccggcaat
     3601 cgcattggct accgcttatc tggtagatgt gatgggcatt gatccagaag agtccgtagt
     3661 tgtttgcccg gttgaccctt acgtagaaga tgactatttt gaggccctga aatctctgtc
     3721 cgatcaggct gacaaaggcg aagctaatct ggtgctcatg ggtattgaac cgacttatcc
     3781 ttccgagaag tacggctaca tcattcctga gagcgcagag cagactgctt tcgtaaatac
     3841 cttcaaggaa aaaccttctg aggctgtggc aaaggaatat atcgcacagg gtgcactgtg
     3901 gaacggtggt gtgtttgtct acaagctgaa gtatgtcatg gagcgtgcac atcagctgat
     3961 tgactttgta gattatcagg atctgttcaa caaatacgat actctgacta agatttcctt
     4021 cgactatgcc gttgttgagc atgaggacaa gattcaggta cagcgttttg ctggtatgtg
     4081 gaaggatctg gggacttgga atactctgac cgaaggtatg gaggaatcca ttatcggtaa
     4141 aggtgttatg aatgagaatt gcgatggcgt acacatcctg aacgaactgg atgttcctgt
     4201 gttggctatg ggtctgcatg atgtggtcat ctgtgcatct cgggaaggta ttctggtttc
     4261 cgataaggaa cagtccagct atattaagcc tttcgttgac aagttcgagc agcagatcat
     4321 gttcgcagag aagtcttggg gtagttataa ggttatggat gtagaagcag agagcatgac
     4381 cattaaggtt actctgaatg ctagtcactc catgaattat cacagccaca agaaccgtga
     4441 cgaggtgtgg gtggttctgg ccggtactgg taagaccatc gtggatggat atgagcaggt
     4501 tgttaaggct ggcgatgtga tcaccatgag tgctggctgt cggcatacca ttatggcaga
     4561 tactgagctg aagctgattg aagtacagct tggcaaggat atcaatgtta aagataaaca
     4621 gaaatttgag ttagagtatt aaaaaacgga ggagggctcg ttatacagaa accgttttta
     4681 ttgaagcctt ccgcaaagga ttatctgtgg gggggaaccg cctgaatgat gaatttgcaa
     4741 aaggaattga gatgtctcct cttgcggaaa cttggaatgc tctacgcacc cggatggacc
     4801 gagctttgtt gtgtctggtc agagtatcag gggatgacct tgacggaggt gttgcaagaa
     4861 cacccagagt ttctgggaac gcatccgcag accaaaggag aattgccgat cctgatcaaa
     4921 ttcattgatg cgaaaaagga tttgtctgtt caggtacatc ccacagatga atatgcaatt
     4981 gaaaaagaaa acgggcagct tggaaagaca gagatgtggt atgtactgga tgccagctta
     5041 atctatgggc tgcagaaaat aacagaagaa gctgtgatcc gtgaagcgat tgctcaggga
     5101 actgttgagc gttatcttca gaaggttccg gtcaaaaaga atgatgtgtt ctttattgag
     5161 gcgggaacga ttcatgcgat tggtgcgggt gcactaattg cagaaattca ggaaaattcc
     5221 aatctgactt atcgtctcta cgattatgac cgtgttgaca aaaatggtaa aaagagagaa
     5281 cttcatgtag acaaagcgct ggaagtagcg aacttgaaag ggttgtcaga accgaaacag
     5341 ccgcttcgtg ttctaaaata tcgtgctggt gtagctgcgg aattgctttg cagatgcaag
     5401 catttcgagg tctctcgtat gattgtcaac actgagagaa gacagacggt acactatcgt
     5461 gcggattcaa tcagcttccg tgtgttgctg tgtgtcaatg gctgtggtac cgttcgattt
     5521 gataatgaat cgttggacat ctataagggg gactgccttt ttgtgccggc cgattctact
     5581 gaaatcagca ttcacgggtc gattcagttt ctggatgtga gaggataata atatgaatta
     5641 taaagtagaa tatgagcgtt ggctgaatca tgccaatgca gatattatag aagaactgcg
     5701 ttcctattct gagaaggaca ttgaggactc tttctataaa aatttgtctt tcggtactgg
     5761 tggtctgcgt ggtaccatcg gtgcaggcac taatcgcatg aacgtacaca ccgtaggtaa
     5821 ggcaagccaa ggtctggcag actatctgaa taagaactat gaagccgctt ccgtggtggt
     5881 tggctatgat tcccgtatca aatctgatgt gtttgcaaag actgccgcag acatctttgc
     5941 tgcaaatggt attcaggtgc acttgtggcc agaactgctt cctgttccta cagtatcttt
     6001 tgcgactcgt tatctgaagg cagatgccgg tgttatgatt acggcatctc ataacccttc
     6061 taaatataac ggctacaagg tatacggtgc agacggctgt cagattacta ccgaggctgc
     6121 tactgcaatt ctggcagaaa tcgaaaagct ggacatcttt gctgatgtaa agatggatgg
     6181 cgatgcttct aagattgcat ccattcagtc ggaagttctg actgctttca tcgaagaagt
     6241 gaaggggcag tccgtcctgt ttggcgaaga agtggacaag aatgttgcta tcgtgtattc
     6301 tcctctgaat ggtaccggtc tggtgcctgt tacccgcacc ctggcagaga tgggatacag
     6361 caatgtgacc gttgtggaag aacagcgtct gccggacggt aatttcccaa cttgtcctta
     6421 tccgaaccct gaaattaagg aagctatgtc tctgggtatg gagtatgcca agaagtacaa
     6481 tgcggatctg ctgttggcca ctgaccctga ctgcgaccgt gttggtatcg cagttaagag
     6541 cgcacatggt gactatgttc tgttgtctgg taatgagact ggtctgctac ttctggacta
     6601 catctgttct cagagaatta agcatggaaa aatgcctgct gaccctgtga tggtcaagac
     6661 catcgtcacc atggatttgg gtgaacgtat cgcatccaac tatggtgttc gtaccatcga
     6721 tgtgctgacc ggctttaagt ttatcggtga gcagattggt atgttggaaa aggctggtaa
     6781 ggttgacagc tatatcttcg gctttgagga atcttacggc tatctgtccg gtagctatgt
     6841 ccgtgacaag gacggtgtta acgctgctta tctaatctgt gaaatgttct cttactacaa
     6901 gactcagggt atcagcctgc tggataagct gaacgagatt tatacaaagt atggctattg
     6961 tttgaacacc ctgcattcct tcgagtttga cggtgcggca ggttttacca agatgcagga
     7021 aattatggtt gaattccaca agggtctcga tataattggt ggtaagaagg ttattgaaac
     7081 tttagactac agtaagggcc tgaatggtct gccgaagtct gatgttttga agtttatgct
     7141 ggaagataac tgctctgttg tggttcgtcc ttctggtact gagccgaagc tgaagaccta
     7201 tatcagcgtc agcgcagaga acaaggaagc cgccgaagct atggaagcta ttatggctgc
     7261 tgaactggcg gaaatgttcg agtaagaaaa gaaaggagaa atcttgtaat ggaggaactg
     7321 ttttctaacg ctttgaaatc ggtgaagtca aatcgtgtgc tgtacacggc atcgttcttt
     7381 gcaagatcct ctcttttgta tttgcaggaa attggacagc tgactgctct gaaggcacat
     7441 acatctaggc gtacaggatt gtcatcttac ctttgtttcc tggtagaagt tggttcaggc
     7501 acattggagt ataaggggca gaaatatacg cttcaagtag gagatatggt gtttattgac
     7561 tgtttgaagc cgtatgtcca tgagtcatca gaagatgatt tgtggacttt gcggtggtgc
     7621 cattttaatg gcattggtat gacggaagtc tacaaaaaat accttgaacg aggtggtcaa
     7681 ccggtcttta aaacatcaaa aacagataag tatgctgact tgctgaggag ccttcatatg
     7741 gtggcagagg gacaggagta tgtccgggat atgaaaatca atgttctact cagtgaactg
     7801 ctgatgcatt tgatggaaga ttcttggaat cctgacaaag tgccgaattt ctctacaaaa
     7861 aaaatggaat tgcagaacat aaaacaatat ctggatgacc attacacgga gaagattacg
     7921 ctggatcagt tgagtgcaga gttttttatc aataaacagt acttagctaa gatttttcgt
     7981 gaaacttacg gtacaactgt tgtcggttat ctgaatcaga aacgaatcac acaggccaag
     8041 cacttgctac gctttacaaa tctaagtatg gaagagattg caacaaaaat tggcataaca
     8101 gatgcaaact atttttccag aacttttttc aaggtggaag gtgttcgtcc cagcgaattc
     8161 cgcaagatgt ggtaataaaa agccggcggt gttgagccgc cggtcagtac ctgtcgttaa
     8221 cccgcattca aagctttaat ctcagctttg atttcagcac gaacacgata gtaatcttca
     8281 gtttcgattt cggtatccag ccgagttgct gtcttttcgc agtcaggaca acgctctggc
     8341 tgttcgtcgg cagggaagct gtagtggcag gaatcacagg tgtaaatgtg atcgtcacgt
     8401 ataatcatca cctaccaatc cagtgcttag atttttcatg caattactat tatatcgaat
     8461 tggtgattcc acaaaaatcc gcttcttgat aaatgtacga ttcagagtga tatatagtac
     8521 tgtactgcca aagggaatac cacaacggca gaaatggatg attcacatgg aagaaattat
     8581 gcagatgatt ttagggaacg caaaattcca gctcggacag gtggtttcga cccctgctgt
     8641 attggaaact ttcaaccccc tgcagaccgc aaacggcatt cccgaagcct tgagcaacct
     8701 ttggtttaga caggaaatta gtattgcgga gatcgaggac gcagttctgg atgccaagaa
     8761 cggcaaggag ctagcaaaca gcctcaaccg catcaagctt ttccgcaagt tcaccctcga
     8821 ccgagagctg gacggcatgg tcagactgaa gggcacggac gtctggaaga acatcagcta
     8881 cctcaaggtc tacaccgcca cgccgcctaa gaaaccgacc cccccaaggt tccagtcgaa
     8941 cggctggagc cttttcttct ctcataaatc ggcataatgt atacaaatac agctccaaat
     9001 gattgtgtag tatatgcctg tggattgact tgatatagtc gagagactat ggcaatatac
     9061 acacaaccaa aggggaaaat cccaaaaaca aggaggaaac gctaatggca atgagcgaaa
     9121 aggcaaagaa atatttgaag gagatcaagg gtgcaaagac catcccggag ctgaaggatg
     9181 tggagatcgc aatcaagcga gacggtatcc ttgcttgggc agagttcacc aagttgaacg
     9241 aggcggtgga agagaagaag gtcgcccttc gcaagaaaaa gcaggagacc agccttcagg
     9301 agatcctttt ctgggcctac aaaaaggaat cagacaagct gctgaagatg atggacgagg
     9361 gagcagacaa ggatgccatc cagatgcagg tccagcgtta cgacagcatc ggccagatca
     9421 ttgcagaggc agacctcgaa gatgagtatg aggtttgaga atacagcctg aaccactaag
     9481 aaccaaatac cgaaagggac ttctgaaaca gacagaggtc cctttctttt gctcactttg
     9541 gcggtcaatt atgaaccaaa aaccagtcga atgattgtgt acattatggc tttcaaacga
     9601 cttgataata atcactttta gagtgatata tgtactacaa aaaaacaaaa gactggagaa
     9661 aacaacagat gagcgaatac agaatcattt atatcagcga cgaggacggc ggcgaggtgg
     9721 aaggatgtgg ctacacggct acgatggtta tgatgcggtc agaaacttct tggaatttgc
     9781 ctgcggcatc ttgaaaatcc tgcgggtgcg attcatcaga agagtggact gaaatacaag
     9841 ttagcagata gagaagagag tcaggtttcg acttggctct gttctctgtc tactggcaga
     9901 aatacattga agggaggtga cgactgtggc aaccagagga agaaaatcaa ccccgacagc
     9961 catgaagaag ctggagggaa atccgggcaa tcgcccactc aatgcaaaag agtcaacgcc
    10021 ggaaaagaag gcaccgacct gtccgaagtg gctggaacca gaggcaaaga aggaatggaa
    10081 gcgtctggca aagcagatgg aggccatcgg aatcttgaca gatgtggata tggctgcctt
    10141 tgccggttag tgccaagcct acgcccgctg gaaggaagcc gaggaattca ttacccagca
    10201 cggtatcatc gtgaaaaccc tatccggcta ttggcagcag gtaccacagg tgtttattgc
    10261 ccagacctat ctgaaaatta tgaaccgatt ttccgagcag ttcggtctga cgccttcgtc
    10321 cagaagccgt atcattgccg gtgatggcgg tggtgtcgga gcagaagatg cgatggaggc
    10381 tctgttggga ggtggtagct gatggcagaa accagaccaa agggctatcc caaattaaag
    10441 gaatacacac caagccgatt catgctttcg gaatgtcact acgacaaagc cagagcggat
    10501 cgggcggtga actttatcgg acagctccga cacaccaaag gcaaatgggc aggcaaccgc
    10561 ttctggctct tgccttggca ggaacagatc atctagtgta atctcgctta actgtattaa
    10621 cggacgtagc gactttatta agagtgttca tggtaggagc attagttgca acaatgattg
    10681 atattaaatt tttatggtac caacgattgg cgatgtgaca ttgaatatgc aaaactatat
    10741 cgatatgata ttatctaaaa tcttactcgc tgggatcgtc gaggtgttta ttggttgtta
    10801 ggaaagaaaa atatgaccca actcgagcga tttttatcgt attacttgtg tgtggcttta
    10861 tctgcattgt gtgtgatttc gaaataaggg agagccaaaa atgagtagaa aattgatatt
    10921 gattagtcac ggaaaatttt gtgaggaatt gaaaaaaagt acaaaaatga ttatgggacc
    10981 tcaagagaat atctacacgt tacctttatt accagaagat ggcactgaaa catacagaca
    11041 aaagctctta ctgtcataga ttcgttaaat gattttgtgg tactatgcga tcttttaggc
    11101 ggtacaccta ctaatatcat ttctaaactg ataatggaag gacaacagat taaactttat
    11161 gtcggattaa tcttcctatg gtcattgaat ttatcaactc gcaagtgatt ggttcagagc
    11221 ccgagtttgt tcgtgcagga tgtgaaagca ttgtatctgt caactaatta attagtggaa
    11281 tagtagaaga tgaagagtaa taaaaagaga gtgggacaat agtcagaaaa aaatctgatt
    11341 attgccccac tttttttatt gactttagtc agtttcgttg gcgtagcgta cgaaacaaaa
    11401 aaacgcgcgt ttctacgcgt ggacgaaata gctttagcga taaaagttta gcacatacga
    11461 gatgggattg taagtatcac atagtcttta ccccgaagta tcgacgaaaa gtgatttatg
    11521 gaaagttaag agtaagtatt ggccagatat taagaaaatt gtgtgagagg aaagatgttg
    11581 aaatcattga agctcatgca atgcctgatc acattcatat gttagtaaga ataccgccaa
    11641 agattagtat ttctagtttt atgggctact taaaaggacg aagtgcaacc ttaatccatg
    11701 aaaaacatgc aaatttgaaa tataagtatg ggaataaaag cttttggacc aaaggatact
    11761 acgtgagtac ggttgggtta aatcaaaaaa caattgaaat atatatttga gaacaagaag
    11821 cagatgatcg aattagagac agtataagta aacaagagta tgtagatcca tttaaatagt
    11881 taaagagaga tagggctgtg agtggtgagc ccttctaggg ctgttaaaaa gccacccgtt
    11941 ttcacggatg gatatttact gattttaagg tacacaaaaa gcactcatcc ctgtaggatt
    12001 aaagtgacca aacccaatca gaaaggaagc gagtgcttta tgtttaaaaa ttataccatc
    12061 aaccaaaaaa ttttaccatt agatatggaa caatatattt ctgaaacaga tgtcgctttt
    12121 gcggtcaacg ctttagtcga agaaaagata aaagtggatt tatacgtgtc ttcaaaattt
    12181 acgaatgtga aagtggtcgg tgctgacctg ttcgtagtca atgtacgaaa gcaaaaagtg
    12241 agcgaaaccg acaaatttta gtgaataata catggcgtta tttcaaagca gaatgtaaaa
    12301 aaacttttag aggaaaaaga aggttcgatc tatcggaaac ggatttccac ttatgaagga
    12361 aacaaggagc caagattgac attggcttgg cactcatggc gttaaattaa gaaaattagg
    12421 aaaatacatg gagaggaaaa gacgtaaaga agaaaaaact attgtcccac tctctaagac
    12481 tgtagacaaa agcatcagtt tcctctttac taagagtgta gactgatact tttgtctatt
    12541 tttgtataat aaaagatata acgacatatg ctcccactgc caagtagcta attttttcag
    12601 attcattgcg acaaaaacaa gcattgtatg cacctgattt tttccaagcc tcgatacttg
    12661 gtgtagcgca ttccatgttt ttcctttgcg tctgcaaaaa tgcgttcaat cgtttctttt
    12721 ctttttttat atttttcacg attgtacacc gtatggcgta aatgttctgc ttcatctaag
    12781 tagtcttccc aaatatgtcg atgtatttct ttttggtgtt tttggctttc tgtacaattt
    12841 ttcaataatg gacaactttc acaaataaaa gatttagata catatttttt atagccatct
    12901 cgagtagttg tgcgaaactg atataaggga gaatgggtcg tagttttcgt tcgattaaat
    12961 acttgacgtt ccaaggtgtt ttgtacgcag cattagcagc tacaccataa cgtgatttaa
    13021 cgcgttcgat ttgtggcacg agcattcggc tatcatgtag atttccaggc gtaatcatga
    13081 catctaatac aaaaccattt tcatcaccaa cggtatgagc agaataagca aactgttttt
    13141 ctcgttctgt ttttacataa taaccacttt ctggatctgc gatgctaaat tttctttcga
    13201 ccacctcctg acgaatgatt gaaccaaaag gacgtttgcc ttcggcaatt cttgcctcat
    13261 tgatttcttc ttctaattgt ttttggtata aaatggcttc tttatgtgcc atatctcttg
    13321 taaaaatacg tttgttcgca ttcgctttaa tatgagttga atccatatac aaaatttctt
    13381 catgaataaa tctagcatca actgcttatt cgagaataat gataaatatc tgttcaaaca
    13441 aatcggtttc tctgaaacgc cgtgcatagt ttttaccaaa agttgaaaaa tgaggaactt
    13501 tatcaattaa actaagccca agaaaccaac gataagctag gtttgtttca acttatttaa
    13561 tggtctgacg catcgaacaa attccaaata aatattgaat gaatactaat tttactaaga
    13621 cgacaggatc aacgctaggg cgaccaatgg tcgagtaagt tgattcaaca atgagataaa
    13681 taaaatcaaa tgacaataat ttgtcaattt tacggactaa atgattttta ggaacaagtg
    13741 catctagcga aattttgtct tcttccattc gactatacat tgattatttg gacatcatta
    13801 tacattcctc ctcgaaatgc gaacgttttc tttaattgta acaaaaaaac tgtagaacaa
    13861 agaaattttt ttatttgtct acagtttttt gtcatatatt tttatttccg cttctgctgc
    13921 cttcctgcgt tgcggccttt gttgtttgta ctgttttttg gttgtttgat ttgttttgtt
    13981 gcttgtttgt tgtcagctgg ctttgctgct gattctgtca cgtcttttct tggagtaacg
    14041 acttgttttg gcgggttttg tttcaactct tctgccactt gtgctttcaa acgcggtttc
    14101 attataacgt ttgtgatgaa tgtttgcaga cagctgaaca caccaccgac tacccagtaa
    14161 agtgtaacac cggctggcgc actgatcgac atgaagacga tcatagcagg agagacgatc
    14221 agcatcgtac gcatcgtctt tttctgttct tcaggaatac cgattgttga aatatatcct
    14281 tgaagcagat aagcgccccc tgcgatggcc acaagcacca tacttggtga gcctagatcg
    14341 atgccgtaaa aagatgattc acggatacct tctgtgtagc gagccgtaaa atacaaggca
    14401 gagaagatcg gcatttggat caatagaggc agacagccga ttccgccggt catactcaat
    14461 ccattttcgc gatatacttg ctgcatttct gcttgtgctg ccatttgttc ttcacgtgtt
    14521 gttgcagctt ttagcttttg ttgtgctgct tcaacttgtg gttttaacgc ttgcattttt
    14581 tcagattgga tcattgtttt tttcgattga ctgataccta gtggcaagat gatgatacga
    14641 acgatcacag tcattgcaat aactgcccag ccgtaagacc aattgaaatt ctcaactaga
    14701 tatgtaatcg cttggcccat aggatggacg agtactcgat aaacgattcc tgaagtgtca
    14761 gggtttccgt ttgaatccat ccgcacacag ccagataaaa agagcaggat actgacaagt
    14821 ccggatccca ataaccagtt tttccattta ttcatagaat acttcctctt ctataatttt
    14881 tacacaaact taactatact aaaaaagcgc atggaattca atcaacttca tagaaatttc
    14941 atataaatat tcgttctttt tcgtagcgaa aaattttgaa aaaagacaaa aaatcaagaa
    15001 agaaatactt agaaagaaaa gagtgattcc aagtgtttct ttcttattta aaaaactaaa
    15061 aacttttttg ttctgtctgg aaaatatggg ttaagcaaat ttattgcaag ttatagatca
    15121 gcttttttac tacctgtctt taatacttga ggcaaagtgc ctcaaaaaca accttatcga
    15181 cgatgttcaa aagtcagttt ct
//
LOCUS       DBNIBO010000056        15201 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.059, whole genome shotgun sequence.
ACCESSION   DBNIBO010000056 DBNIBO010000000
VERSION     DBNIBO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.059"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(149..1318)
                     /locus_tag="AC1Y77_001464"
     CDS             complement(149..1318)
                     /locus_tag="AC1Y77_001464"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIQ1575043.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1522..2697
                     /gene="mltG"
                     /locus_tag="AC1Y77_001465"
     CDS             1522..2697
                     /gene="mltG"
                     /locus_tag="AC1Y77_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIQ1575044.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2833..3312
                     /gene="greA"
                     /locus_tag="AC1Y77_001466"
     CDS             2833..3312
                     /gene="greA"
                     /locus_tag="AC1Y77_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIQ1575045.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3481..4212
                     /gene="liaF"
                     /locus_tag="AC1Y77_001467"
     CDS             3481..4212
                     /gene="liaF"
                     /locus_tag="AC1Y77_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HIQ1575046.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4209..5276
                     /gene="liaS"
                     /locus_tag="AC1Y77_001468"
     CDS             4209..5276
                     /gene="liaS"
                     /locus_tag="AC1Y77_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HIQ1575047.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5283..5915
                     /locus_tag="AC1Y77_001469"
     CDS             5283..5915
                     /locus_tag="AC1Y77_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1575048.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6055..6720
                     /locus_tag="AC1Y77_001470"
     CDS             6055..6720
                     /locus_tag="AC1Y77_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIQ1575049.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6748..7041
                     /locus_tag="AC1Y77_001471"
     CDS             6748..7041
                     /locus_tag="AC1Y77_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HIQ1575050.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7109..8056)
                     /locus_tag="AC1Y77_001472"
     CDS             complement(7109..8056)
                     /locus_tag="AC1Y77_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIQ1575051.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8234..9037)
                     /locus_tag="AC1Y77_001473"
     CDS             complement(8234..9037)
                     /locus_tag="AC1Y77_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HIQ1575052.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9183..10097
                     /locus_tag="AC1Y77_001474"
     CDS             9183..10097
                     /locus_tag="AC1Y77_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIQ1575053.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10145..10615
                     /locus_tag="AC1Y77_001475"
     CDS             10145..10615
                     /locus_tag="AC1Y77_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIQ1575054.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10700..11308
                     /locus_tag="AC1Y77_001476"
     CDS             10700..11308
                     /locus_tag="AC1Y77_001476"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIQ1575055.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11442..11642
                     /locus_tag="AC1Y77_001477"
     CDS             11442..11642
                     /locus_tag="AC1Y77_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1575056.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12396..13622
                     /locus_tag="AC1Y77_001478"
     CDS             12396..13622
                     /locus_tag="AC1Y77_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIQ1575057.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13730..14200)
                     /locus_tag="AC1Y77_001479"
     CDS             complement(13730..14200)
                     /locus_tag="AC1Y77_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIQ1575058.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            14522..15100
                     /locus_tag="AC1Y77_001480"
     CDS             14522..15100
                     /locus_tag="AC1Y77_001480"
                     /EC_number="1.14.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase auxiliary
                     activity family 9 protein"
                     /protein_id="HIQ1575059.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         5190 a         2347 c         3024 g         4640 t
ORIGIN      
        1 acctctttct gttctgttta tttggaatta aagtctgtag caaaaattga tttttagatt
       61 tgtcccacac tctttcctac gtttagtcga gtactttttt taagcaaatg ctattcaaag
      121 aagtagtatt tgctatcttt tcatcatatc agctatttgc caacttagta gttccttcca
      181 tcaggtcata gatttggttt cttagatcag cagtaggcag tttgatagtt ttgtcagctt
      241 ttatattgct ttccatgatg atagcatcct gtgaatcttt gctaaatata gcagatgtat
      301 caaaagagtc gatatttcct tgcgacagga tataattatc tccgctatat accccaccac
      361 tatatggatg tccattcgca ttttcacgcc ataatgagaa gagtaagtct ttggaaacta
      421 gttttccaga taaaacttgt gtaaacagcg tatttaaatc atagacggac atactgatat
      481 taccagtccc taattcgtta tggatctctg tttcagattc tgtcagtggt ttaaagtaat
      541 catcctcttt agacatttga taggatagtg catgattgga tgaatttttg acttggtcat
      601 aaaattttgt atgctgtaat cctaaaggtt cgatgatatc atcggtcaat aatttttcat
      661 acggttgatt tgttaatttg atcaaaattc cagtcaatat ggtaaagttc aatgaatcat
      721 aatgagaggt ctgataatct gtaaatttta agtgctgaat cgcaaattga acgacttctt
      781 catcagtcat cggtatggtt ggagaagcag ttcggtgcaa gcctgagcgc atatacagca
      841 agtcacggat agtaatttgc tggctgccag caatttgtgg atagaattga gccagcttag
      901 tatctaaaga aaggtgccca cgctcgactt gttttaaaat caaaagagta gtcaatgctt
      961 tttgaaccga accaatctga taataaagag aaggatcatt tggttgttgc gtgagtttat
     1021 tcgcatagcc atagcctttt tccatgatga tgcgattatt tttctttacg taaattgagc
     1081 caatataatc attttttctt aatagttgat cgattttttc aacgagctgt tgttcattgg
     1141 tagtcagtat caactttttt tgagctgatt gagcatgggc aactatctga gagtgttcat
     1201 tattgtaaaa ttgccatcca agaatagttc ctgtagagaa cgcactaagg ctaaacaaca
     1261 cgacaacgat cattaaaaat gaatattttc tttttttagc atgttttcct tttttcataa
     1321 tagtttattt agtctccttt cttttttaca gaaagaataa agctaaaata gcttgtatct
     1381 tgataacatt tattttaatc ttatagtaat ggatttacta cttacatttt tgatagaata
     1441 tttaaataaa atcattcacg ccaattcaaa tatcatgtat aatgaataaa gttacgaact
     1501 tgatagtaag gaggagttgt tttgtcagga aacaaacaac acccaagtaa agataaaaaa
     1561 gaagccagta aggcagctac agcagatata atcgacaagc ggagaaatct gcgtaaaaaa
     1621 gaagataaga ttgtgaggaa gatcatttta gtgattgctc ttacattatt gattattggc
     1681 ggatttcttg gatttaccgt gtatcgttat gtagatagcg ggttgaagcc tttagataaa
     1741 tcagatgacc aattagtcca agtcgagatc ccgagtggct cttctaacaa gcagattggt
     1801 gagattttgg agaaggacaa tattattaaa agcgggattg ttttcaatta ttatacaaaa
     1861 tttaaaaatt taactggctt tcaagctggc tattatcagt tagcgcctaa tatgactctt
     1921 gacgagattg gcaaacagtt gcaagaaggc ggtacttctg agccaacaaa agttgcagat
     1981 gggaaaattg ccattccaga agggtacgat atcgatcaga ttgctgaacg ggtagcgaag
     2041 gtaactggta aagacaagaa agaattttta gatttagtga atgatgagac tttctttaac
     2101 gagttgcacc aaaaattccc agaactgttg gaaagtgcat ctaaagctga gaacacaaaa
     2161 taccgtttgg aaggttactt attcccagcc acatatgatt attacacgag tacttcgctt
     2221 aaggatctag tgattgaaat ggtgaacaag acgaatacgg tcatgcaaaa ctattattca
     2281 gctatcaagc agaaaaattt gactgtacaa gaagttttaa ctctagcttc tctagttgaa
     2341 aaagaaggcg taaaagaaaa tgatcgtaaa aacattgcac aagtcttctt taaccgaatc
     2401 aaagcaaata tgccacttca gtcagatatc tctgttttat acgcattagg tgaacacaaa
     2461 gaattagtga cgtatgaaga tacagctgta gattcaccat ataatttgta taccaatacc
     2521 ggctatggac ctggaccttt tgacaatcca agcgaagagg caatcaaggc agtattagaa
     2581 ccagcagaaa atgactatta ttatttcgta gcagatacga gcactggaaa tgtttatttc
     2641 gctaaaactt atgaagaaca tatggaatta gtggaaaaat atgtaaataa ttcttagaaa
     2701 aacaggctct agacattgat catttttaga aaaacattta catttgaaga aaagcgtggt
     2761 aatctttttt ggactatatt cctcaaaaga ttaccttttg cggtatttta tcgaaaaaag
     2821 gagacgttac acatggtaga gaaagtttat ccaatgactc tagaaggtaa agaaaaatta
     2881 gaaaaagaat tagaagaact aaaaacagtc aaacgcggag aaatcattga acgaatcaaa
     2941 attgcccgcg gtttcggaga tctttccgaa aactctgaat atgaatcagc gaaagatgaa
     3001 caagcatttg ttgaaggaag aatcacaaca attgaaaata tgattcgttt tgctcaaatc
     3061 atcgataacg acggtgtaga tgcagatgag gtatcaattg gtaaaacagt tacattcatc
     3121 gaactaccag atggagaaga agaagagtac acgattgtcg gaagcgctga agcagatccg
     3181 ttttctggca aaatttccaa tgattctcct attgcgcaag cattgattgg caaacgtaaa
     3241 aatgatgaag taactattgc tactccaggt ggcgatatgt tagttaaaat cgtcaaagta
     3301 gaacaagcgt agtctgactg aaagatctag agagttcatc ttactgatga tacactctag
     3361 atctttttct ctttgtgtat tttagatttc aacaaacgat cttacatcta aagtcctata
     3421 gccaacagtt aaaaaaatag gtatgatgaa atgagaatgt gtcaggaggg agaaagagcg
     3481 atgaatagtt cttggcgttt ttttgtggta gttgaagcac tactgctgtt atttgctgtt
     3541 tggcaaatcg taaacaatac tggacttttg cttttagtga tattcggtat tttcaatata
     3601 tatctagcta tgcgcaaaca tcctcgtact aaatttcaaa attttcaatt gatattagga
     3661 agtttggtca ttttttttag cttagtcaac agtcctgctt tatggatgat ggctgtattt
     3721 gccgttttat ttattggtct gaaaggtgta gagatttcag ggatagattt aaccaaaaat
     3781 gctttttggc gaaagaaaca gatcatgatg atccaaacag agcaattaaa aacacataat
     3841 aatgagcgga aaagacagca attattcggt aaccaacgta ttggaaacga cgtctatgaa
     3901 tgggacgata tcaacatcgc cgtcatttct ggtgatacta tcatagattt gggcaatacg
     3961 cttttgccaa aagatgataa tattgtgatt gtaagaaaag ggataggcag aacacggatc
     4021 ttagtacctt tgggcgtagc catccgatta gagcatgcga cattagtagg aaatgtcttg
     4081 tttgaagaag agcaattttc tttgaaaaat gaacaaatca aaatctacag cagtgattat
     4141 gatgaaaacc ctcgtagatt gaagattatt actaatacct tgattggaga tgtcgaggtg
     4201 atacgaatat gatgggaaaa atatccagag cgatgctagc tgtttattcg gggattgcgg
     4261 ctttccttat tattctattt tcacttttca cttattttta tgccagcaat caaagtcatt
     4321 ggtggggaga attactgcgt gcacgtttat tatatgttcc gcttatcttc catttgttag
     4381 ccatttcctt aggtgtagga ttaattgtct ttctattatt atcacttatt caaaagacaa
     4441 aatacgggaa aatcgaagaa aagctgcgtg cactttcttc tggcaattat gaatccaaat
     4501 tgttgcttct tccgatccca agtgcatcgg acgatttata catcaaagat attgataagg
     4561 aaatcactaa gataaaagaa aaaatgattg aagtatctag tgaattacaa attgtaacga
     4621 gccgtcccca atatgtagat ggacaaacca aagaagaaat tttagaatta gaaagacatc
     4681 gattagcccg tgagctgcat gattcagttt cgcaacaatt gtttgcagca atgatgatga
     4741 tgtcggcatt gacggagcaa gcagaaaaaa gcgagacacc agaaatgttt cgcaagcagt
     4801 tgaaaatggt agcagagatc atcaacgctt cccagtctga gatgcgtgcg ctgcttcttc
     4861 atctacgtcc agtcaattta gaagagaaaa gtttaaagca aggtatcgag cagttattga
     4921 aggaattgca aaataaaatc cagatttcgc tgaaatggga tgtagaagat gtaaaactat
     4981 ctagttccat tgaggatcac ttgttccgaa tcgttcaaga attgttatca aatacattaa
     5041 gacatgctaa agccaatgaa ttagaggtat atttgcacaa aatagacaat aatcttttgt
     5101 tacgtattat tgacgacgga acagggttta atatgaatga aacgaaaacg ggaagttatg
     5161 gattgaacaa tatcaaagaa cgagtagctg gtattggtgg aacagtaaaa atcattagtt
     5221 ttaaaggtca aggtacaagt gtagaaatca aggtcccttt gatgaaggag gcatagataa
     5281 ggatgataaa agttttatta gtagatgacc atgaaatggt gcgcttaggc gtctcttctt
     5341 atttatctat tcaagaagac attgaagtaa ttggagaagc tgaaaatgga cgacaaggct
     5401 atgagaaggc gatgacactt cgaccggacg tcattttaat ggacttagtc atggaagaaa
     5461 tggatggtat cgaatcaaca aaagcaatct taaaagattg gccagaagct aaaatcatta
     5521 tcgtaaccag ttttattgat gacgaaaaag tttatcctgc cattgaagca ggtgcagcag
     5581 ggtatttgtt gaagacctct accgcacatg aaattgctga tgctataagg gcaacgcaac
     5641 gtggcgagcg agtattagaa ccagaagtga caacaaaaat gatggaaaaa atgagtcggc
     5701 gaaatgagcc tgtattacat gaagaactaa cgaatcggga aaatgaaatt ttaatgctga
     5761 tttctgaagg aaaaagtaat caggaaatag cggacgaatt gtttattaca ctgaaaacag
     5821 taaaaacaca tgtttccaat atattggcta agctagaagt ggaagaccgt acacaagccg
     5881 ctatctatgc attcaaacat ggtttggtga aataaaaacg atgataaaga ggataatcaa
     5941 aagctgagaa aataactatt atttttcttg gcttttttac aataatgatg cttgcttttg
     6001 tttttgtaaa ggctttgtta tactgagaaa agaagatact aaaggaggaa aacggtgaaa
     6061 caaaattttg caattgtagg tcttggacga tttggcggaa gtatctgccg aacactgatc
     6121 gaagctggtc aagaagtact ggctattgat agtaatgaag atcgggtcaa tgaatatatg
     6181 aatatagcca ctcatgctgt tgtggcaaat gcacaggatg aaatgacact tcgatcatta
     6241 ggcataagaa atttcgatca tgtaattgtc gctattggag aagatattca agccagtatc
     6301 ttagttactc tgatggtaaa ggaaatgggc gtaccaaata ttttagcaaa agcccaaaat
     6361 gagtatcatg ccagagtact tgaaaagatc ggagctgatc gcgtcgtcca tcccgagcga
     6421 gacatgggaa taaggattgc gcataattta gtgtcaaaga atattttaga ttacctagag
     6481 ttgtcagata aatattcttt agcagaaatc cgagtctcca atcctaaatt tttcaacaaa
     6541 acattagctg aattaaattt caggcaacgt tttgacttaa cagtagtcgc catacgaaga
     6601 gtagatcaaa cggttatcgc ttctcctgca gcagatgaat atgttcgtga aaatgataat
     6661 ctactggtta ttggagagac aggcgatgtg gatattctag acgataagat gaatcaatag
     6721 aaagaaaaga gaaagtaggt tgttgggatg aaattaacgg taacgccaaa agcacaaaag
     6781 tggtttgact cagaaattga tctgccagaa acttacggaa tacgtttttt tggaaaagtc
     6841 tacggcaaaa cagaggttca tgatggtttt tcgattggaa tgtcagttga acagccagaa
     6901 catcctgtaa agaaagtgga aatggatggg acactttttt ttatcgagga agcagatgaa
     6961 tggttcttta aaggatatga tttacttgtt gattatgatc ccaacctgga ggagccaacc
     7021 tatcacttta aaaaacaata aaaaaagaga gggtgacaca atttttgtca ccttctcttt
     7081 tttcttttga aaatattgtt ttcagctatt aatatcctgc agacaatttt acttgattgg
     7141 tttgaagctc atgggtggca aagtagcttt tcagattggt taaaaaaata gccattaatt
     7201 tcttttgaaa atcaggtgtc aaacctgcaa tgtgggaagt aatcagtaca ttgtccattt
     7261 tccaaagagg atgatcttgt ggaagaggct cttcctcaaa aacgtctaat gctgcaaagg
     7321 aaagttgctg gttatttaaa gcctgcacca gatcttgtgt cttaacagaa gcaccacggc
     7381 caacattgat gaatacagat tttggatcaa agtgttcaaa catatgctga tcaaagagcc
     7441 cagttgtttc ctccgttaga ggtaagatat tgactacgaa atctacttca ggaagaactt
     7501 cctttagttg atttaaagaa aaagtctttt ccattccttc tgctggatga ccggatgtat
     7561 tgattccata gcagttcatg tctaatgctt tgcatttttt tgcaatcatt tttccaatat
     7621 ggccagttcc aatgattagt atttttttcc cttggataga ttggtaatgg accgatgaac
     7681 ctaaccattt catgtcactc tgtgcctttt gtgcttgaaa aagaccacga gaatacccaa
     7741 ggatgattcc taagacatgc tccgtaatcg attcactgtg gatgccacta ccatttgaaa
     7801 gtaaaatatg tttctttgaa aaagtctcaa ggggtagctg atcaacacca gctgagatag
     7861 attggaccca tttcaaggta gaagaggcta atagtttttc ttggtagtct gagttccagc
     7921 ccaaactgat ctctacattt tgtagatcct ctggttgtaa ttctgtttta aagatataac
     7981 caggcgctat agtatggatt tcttcgataa attcatcacg aaatggacga tctgcataaa
     8041 tgattctttc tgacatagtt aatttcccgc ctttctttac tctaagtgta gcgcatacaa
     8101 gaagaacttt gaagcgcctt gcttgttttt tgagaaagaa atatttttca aataaaaaag
     8161 gggctgtgac aaaaaaatgt cacaacctct aaaagggtct attgaagtag aaataacgag
     8221 ttgtctttat ttcttaattg atcgatattt ttgcccatcc tttgacagct tgagcaaaaa
     8281 taaagattcc tgcaaaagca atgaaggcgc tagtgtcaaa agaaggaaga aagaccatca
     8341 agattgttgc aattatagta ggcaatgaaa ttgaatagat caatgttttt aaacaatcaa
     8401 gaaaagtagc ttttgttagt cgaaagcgtg catagagaaa agcagcaatg ttggtaaata
     8461 gcaaagtgat cagcagattc agaaaactag gataaataga aaatataaat gtcaggactt
     8521 ttatccaaag tggtatagat gcttcactta aaaaagaacg gagctgctta cctgttaagt
     8581 tttttaaaga ttcattggtg tagggaagtt ctaattggtt attgttcaac aaagcagaag
     8641 tcgtgcctgt attaggaaga gctatgataa gtttgttttt taacatcccg acactaacaa
     8701 aattccccat caaatcagta gagatgtcct gttctgttcg ttttccctct ggatcaaatg
     8761 tgaagataat ggagtttgtt tggtaaatga atccttcttt ttcttcggtt tgaagctgcc
     8821 cgttttggat tgtaaaatca ggaatgcgag tagcaatctt ttgcccatcc aattgaatgt
     8881 tgtttaagac ttgaaaaact tgaaaagaga ttggcaaagc cataattata ctcaaaacta
     8941 aaagatagac aatggctttc caaaatggga catttctcgc ctcctttagg tctttgaatc
     9001 gtataaagga actgataatt aattgttttg ttgtcataag tactcctcat ctagtagtgt
     9061 catcacttgc aattctagcg aagtatcaag tgaaatacaa gtgctttaag gaaacagctg
     9121 aatgattggt gaaatatcaa aaaaatgtta ttcttgtcta aagactatcc aatataaata
     9181 aaatggagga aggaatagtg cctacacagc cacagagatc atcaaaaaaa ctatttggag
     9241 tagtggtttt gaatgcggtg atcactttat cagaatttat tggagggatt atgtcaggga
     9301 gtttgtctct tgtttcagat gctttccata accttagtga tacattggca attatttttt
     9361 cttacggggc acaaaaaatg gcccgaaaag aagcaaataa aaaacgtacg tatggctacc
     9421 agcgtttaga aatattgagt gctttcatca actcattcat attgatcata ttatccttgt
     9481 ttttaaccgt tgaagcattc aagcggttca cttcaccaga aaagataaat agtcatttga
     9541 tgctaacagt agcagttatt ggattgctcg cgaatttgtt ctcagtatta ttattacgac
     9601 aagaagcaga tgaaagtttg aatataaaat ctagttattt gcatttgtta agtgatacac
     9661 tgtcttcggt aagtgtgatt atcggagcaa ttttgatccg ttttttcgat atctactgga
     9721 tcgatccagt tattacactg atcatttcga tatatatatt gatagaagcg ataatggtga
     9781 tcaaaaaagc tgccgctatt ttgatacaaa gtgcacctgc tatcgactat gaaaaaatgg
     9841 aacaagaaat tgaagcaatc gatggggtaa aggatgttca tcatgtccat atttggcagt
     9901 atagcgaaaa gataattatt ttcgacggac atattgattt tgaagatcag ctactaagcg
     9961 agattgaaaa gatatatcct cgtataattt ctttattaaa attaaaatat ggtattacac
    10021 atgtgacgat ccaagcagag acaaaggcaa aggatcaaaa aaaattaatt tttttaaata
    10081 aagatgaaag ttattgaaat atagagtatg ataaataaga atttttaata aggtaggttg
    10141 gctcatgaag cagtatcgac agttaaaagg atatttagaa gcaaaaggat attgggagat
    10201 actagtctat ctcttttttg gtggtttggc tacgattgta aattttgtta gttttgctct
    10261 cgcacgccaa tattttgatt taagtatggc cttatccaat tctatttcgt ggttttgttc
    10321 tgtattgttt gcttttgtaa cgaacaaact ttgggtattc cattcaaaat caccgaattt
    10381 tacacatgca cttattgaat gcggtaagtt cttcttctac cgtatccttt cttacggatt
    10441 ggacatgggg gcaatggtat tgttgatcaa tgttatgaac agcaatgagt atgtggcgaa
    10501 aatcatcact caaattattg ttatccttgc gaactatatt ttcagcaagt tgtttatttt
    10561 taaagaaact gaggtttttg aagaagaagt aggtcaaagt gaaaaagacg actaggcata
    10621 acaattgaag aatttccttc tctttgttac tctttgtaat gtagacatta ttaagaaaac
    10681 attgaggagg aattttttta tgacttacac attaccagat ctaccttatg catatgacgc
    10741 attggaacct tatattgacg aagaaacgat gcatctgcat catgataagc atcacaatac
    10801 ttatgtgacg aatttaaatt cagcaattga gaaataccca gaattaggcg aaaaaacaat
    10861 agaagaatta ttatctgata tggacgctat tccaacagat atcaagacag ctgtacgtaa
    10921 caatggtggc ggacatgcta accattcatt tttctgggaa atcatggcac caaatgctgg
    10981 tggcgaacct acaggagaaa taaaagaagc gattaatgaa gcttttggtg atttttcttc
    11041 ttttaaagaa gaattcaaaa aagcagccgc tggacgattt ggttctggat gggcttggct
    11101 tgtaatggaa aatggaaaat tagctattac ctctactgca aatcaagatt ctccattgat
    11161 ggaaggaaag acaccaattc taggtttgga cgtttgggaa catgcttatt atttgaaata
    11221 taaaaatgtc cgaccagatt atattgcagc attctggaat gttattaatt gggatgaagt
    11281 aaacaaacgt ttcgaagcag caaaataacc atttcctccg tcatatcagt cttaaaaata
    11341 atttggaaaa taaaagagcg aaagcttgct agcgttttct aaattgggta taatgttctt
    11401 gcaaggttac ttgcaagaac ttttttggag gatatacatt tatggaacac ggaacggtta
    11461 aatggttcaa caatgaaaaa ggatttgggt ttattacagt agacggcgga gatgacgtat
    11521 ttgttcattt ctcagcaatt caaggagacg gcttcaagag cctagaagaa ggtcaagaag
    11581 tagaattcac gattgttgaa ggtgcacgcg gacctcaagc tgctgaagtg actaaattat
    11641 aataatttgt tgaataaaga cacgacttct tagagagtcg tgtctttttg tctatttatg
    11701 aagtttttag attttttggc taagtaaatg catttgttca cttgacatgt cctaactaat
    11761 tatcaagcaa ggaacatttt cttcgttagt aatggatttt aaagaaattt cagtttttgg
    11821 atgttgaata aaagaccatt tgtgaaacca ctagtataca tactcattac catgtcaatt
    11881 ctattacttt aaaagaaaat cgttttactt tgaagaggag catatctatc cattaaaaga
    11941 tgatggtaat gcaatcacta gttcgagtca ttttaagagc catgcagtga gctggaaata
    12001 aaacgaatag aagatgggtt taacaaaagg accagtcata ggaagaaatt gtcgtgaaat
    12061 cgaatgataa tctgaacggt aggcaaagac tgttaaaaag atgggaaagg tatgtgcgat
    12121 cattttactg cacgattatt tggaaaatac cttgttgttc gctttttctg gaaaaaatac
    12181 ataaaaaaag ataaaacgat tgcgaactgc gtgtattcgg tctttcgatc tttttcacgg
    12241 tatggtatga tagttaagaa ttttgagaaa ttttaaaaaa taataatcgt tacacaaaaa
    12301 tgttttttaa agttttctta tgattaaaaa gtgaatttgc tgattgaaca gttatatgag
    12361 acaatatttt agaaaatgtg aaacagaggt gtattatgaa gcgatggcaa aaagttctat
    12421 taaccttgtt aggggtattc gttttgctga ttgccggatt atcagcatac ggaatcagat
    12481 ttatgggaga agcgagccaa acagtaaaca aaatttctaa aaactctaat cgaatatcct
    12541 ccaaacgttc tgagagagta agcattgatg acaaagaacc tttttcggtt ttgctattag
    12601 gtttagatac cggtggatta ggaagaacgg aacaaggacg ttctgatacg atgatggtag
    12661 taacagttaa tccgaaacaa aaaaaatcaa cgatcatcag tttggaccga gatatttata
    12721 cgaatatcgt aggttatgga acggtagata aattaaatca tgcctacgca tttggtggtg
    12781 tcgaaatggc catggattca attgagcaac tgctcgatat tcctattgat cattatgtaa
    12841 cgatcaattt ggatggcatg gaagatttga tcaatgcggt cggtggcatc aaagtgaata
    12901 ataaaattga ctttactttg gatggtgtcc atgtaccagt aggtgaacaa acattgaatg
    12961 gagaaaaagg tttagcctat tctcgaatgc gacacgaaga tccagaaggc gatatcggtc
    13021 gacaacgtcg tcagcgggaa gtagtgacga aaatcgtcaa taaagtttta agtttagatg
    13081 gcgtaagcaa ttatagaaaa attttaaaag cagttgagaa caatgtgacg acggacttag
    13141 attgggatga catgcttgat gtagcgacga actacacccc ggcgtttgaa acaatcaaac
    13201 aagaccagtt gcaaggcgaa ggacaaatga tcaatgatat ctactatcaa attttaggaa
    13261 aaaatgatct tctttcgatc caaaatgaac tgaagaaaca attaaatata aaaacaagcg
    13321 atacattacc taatttgaaa aatgagaatg cttcaatgat gttctttgat gattcggaag
    13381 atggcgattc gacaaacggt gatggatcga atagtgaagg gtacaccgat tattcttcct
    13441 attcagaaaa cacatatgat cagtaccaag atccgaatac ctattatcag caaacagaag
    13501 atacaagtca gtactataca ccaagtcaag atactcaaag ttatgatcct tacgctggac
    13561 aggaacaaaa tggttatggt tatgaccaga ataatgagta tggaacaaca agtgggtact
    13621 agaaaaaaat agtttggtta ataaagagtg ccacctaaat tagacaaaaa atctaactta
    13681 ggtggcactt tttctatgtg ttaaattaca ggacgtctct ttttagcgct tagtttaaaa
    13741 aataaattga ataattcaat agagttgcga atagtaccca taaataataa ggaatcatga
    13801 gccacattgc caggcgatga tgcagccaca actgatacat aagcactgac aagaaaataa
    13861 ctaaaagtgt aatatcgatt gctgcaatga aaatatttct taaattaaaa aatagagcag
    13921 accatataaa attaaaaatg aactgaatca caaaaagacc gacaagattg tttcgctgtt
    13981 ttttgttgtt gtgattaagt aacaaataaa gagaaatacc catcaatata tacagtaagg
    14041 tccacatcgg cccaaatatc cagctaggtg gtgcaaatgg tggaagttgc aaagaatagt
    14101 aatattttcc gggatttccg ctaagcaacc ctgataaaaa gcctaatatg acaattccta
    14161 taacacaaac ccaaaaacga atatctttta tcatcttcat tttactaact cctttctata
    14221 atattagttt accatgaagg ctcacagcaa gacctgtatt tagtttgtga agtaagaaaa
    14281 aaattgataa ttgttgttat taaaaagttg aacatttgtt tatatgaatg atgtttttga
    14341 aaaagatcat atgatttgat tctcatgttt tctatttgta cttttctcgt ctacttctgt
    14401 tataatctgc atcttacttt tttcaccttt tttacttcaa cataaaatta taaaagcata
    14461 atgtttgtgt taatacaata aagcgtttac aatgtggtta tcaaatgaag gaggtttcta
    14521 catgaaaaag attagtacgt tattgtttat tgggttagtt agtttagggt tgttcggttt
    14581 tagcattcca ggttttgcgc atggttatat tacttctcca ggaagccgtg catacctagg
    14641 aacatcagca gcaggaaact taaatcaaaa tgtcggaaga gcacaatggg aaccgcaaag
    14701 tatcgaagct accaaaaata cattcattga tggaaaaatt gctagtgcag gagtcagcgg
    14761 ctttgagcct ttagatgaac agacatctaa cagatggcat aaaaacatgg ttaatccagg
    14821 aacattaaat attacttgga atttaacagc acaacatcgg acaagtactt gggattacta
    14881 cattaccaaa ccaacttgga atcctaatca acctttgaaa tttagtgatt ttgaattgat
    14941 cacaaaaata gacgacaaag caactgttcc accaaaaaca gtcaaccaaa cgatcacact
    15001 tccgcaggat cgaaaaggat ataatgtgat tttagcagta tggaatatca gcgatacgac
    15061 aaatgcattt tatcaagtga tagacgtgaa tatccaataa taatcaaaaa gggctgtgac
    15121 aaaaactatg tcacaaccct ttttttaaat aaatagtgtc ctcctgttaa ctcttcggtc
    15181 acaagtacag tattcataaa a
//
LOCUS       DBNIBO010000057        14936 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.061, whole genome shotgun sequence.
ACCESSION   DBNIBO010000057 DBNIBO010000000
VERSION     DBNIBO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.061"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(85..1353)
                     /locus_tag="AC1Y77_001481"
     CDS             complement(85..1353)
                     /locus_tag="AC1Y77_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIQ1575060.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1584..2321)
                     /locus_tag="AC1Y77_001482"
     CDS             complement(1584..2321)
                     /locus_tag="AC1Y77_001482"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1575061.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2388..2957)
                     /locus_tag="AC1Y77_001483"
     CDS             complement(2388..2957)
                     /locus_tag="AC1Y77_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIQ1575062.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3085..3957
                     /locus_tag="AC1Y77_001484"
     CDS             3085..3957
                     /locus_tag="AC1Y77_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1575063.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4299..5171
                     /locus_tag="AC1Y77_001485"
     CDS             4299..5171
                     /locus_tag="AC1Y77_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1575064.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5168..7126
                     /gene="nagE"
                     /locus_tag="AC1Y77_001486"
     CDS             5168..7126
                     /gene="nagE"
                     /locus_tag="AC1Y77_001486"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HIQ1575065.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7377..8228)
                     /locus_tag="AC1Y77_001487"
     CDS             complement(7377..8228)
                     /locus_tag="AC1Y77_001487"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1575066.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8351..8818
                     /locus_tag="AC1Y77_001488"
     CDS             8351..8818
                     /locus_tag="AC1Y77_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIQ1575067.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9081..9563)
                     /locus_tag="AC1Y77_001489"
     CDS             complement(9081..9563)
                     /locus_tag="AC1Y77_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIQ1575068.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(9663..10526)
                     /locus_tag="AC1Y77_001490"
     CDS             complement(9663..10526)
                     /locus_tag="AC1Y77_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIQ1575069.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            11124..11972
                     /locus_tag="AC1Y77_001491"
     CDS             11124..11972
                     /locus_tag="AC1Y77_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIQ1575070.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(12143..12484)
                     /locus_tag="AC1Y77_001492"
     CDS             complement(12143..12484)
                     /locus_tag="AC1Y77_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIQ1575071.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            12570..13277
                     /locus_tag="AC1Y77_001493"
     CDS             12570..13277
                     /locus_tag="AC1Y77_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIQ1575072.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            13264..13587
                     /locus_tag="AC1Y77_001494"
     CDS             13264..13587
                     /locus_tag="AC1Y77_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIQ1575073.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(13676..14713)
                     /locus_tag="AC1Y77_001495"
     CDS             complement(13676..14713)
                     /locus_tag="AC1Y77_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIQ1575074.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         4361 a         3067 c         2712 g         4796 t
ORIGIN      
        1 tcaaccaaac aaaaaacatg aaaatctgtt tttgtttggc tgttcttact acttgatgca
       61 gggcagcttt ttcactacct ctttttaata accataaaat cctattcctt catatctcca
      121 gcccatcggt gttagccaat ctctttcctt agcactcaag gtatagtgat gggtaccagt
      181 tttttcattt ggattaaata gtcgataaat tggcttggaa ccaccagaat aaaaacttag
      241 accttcataa cgccaaccga tttttgttaa agagttgatc tcagattgtg ccattgtgta
      301 atgatggtct ccactgtaag gattgtataa acggtagaca ggttgtccgc tgtttggtgc
      361 ttgccaagca atcccttcat attcccagcc tacagttggc agataatttt tttctccggc
      421 attcaatgta tataaatgtt ctcctgtatg acggttatac agacgataca tcggagaagt
      481 tgaagttggt gctgtatcat actgtgtcag attataggtt tcgattacat tgttcaatgt
      541 agaaccataa ttaggcgcag tggcataacg acctgttagc caagccgtag catctttgta
      601 agagttcgta ttgcttttcc atgcaccgga atagtagtaa acgcctggct gaaaagaagt
      661 cgtcttcaat accgttacgt tatcttgcaa tgattctttg tacgaaggat attttctaaa
      721 agcggcattc accgtaatcc attgcccatt cacatattcc gaagtaggca tcgtaacaga
      781 ttggccatta tagcttcctt taatcccaaa tagattgtaa ttaggggcag aagccaaagt
      841 actgttcccc cagccacttt caacgatggc ttgagcaatc atgactgatg catataagtc
      901 attggcactt gccaaacttt gtgcagttga ggcaatacta tcaatgaacg cttgttgaga
      961 attagcagat gagcgagcat aaaaagcata gccagtatag ttttcgaatt cagacaaatc
     1021 tgcttgatgg gtatgtgtca atgtttgccc atctgtcaat tggttcgtga tttcttctgc
     1081 attctctgga ttgttctgag cttctgctga tgtactgtct tcggtttgtg tttgttctgt
     1141 tgtttcagtt gttggttctt cggttgaaat tgtattttct gagacagtag ctgactgcaa
     1201 agtactctct gtatcgtttt caattcctgt ttctgaagaa ctcgatgtct gagttgcgac
     1261 agtttctgtc gattcttcag ctagcactgt tgtgttggca actgctagat ttcctaaaag
     1321 aataacgcta aataataaat tctttgcttt catctttttt ctcctttgaa ttatgaaact
     1381 tttcctaaat aacagtgtaa ctaaaaaagt aaaagcaagt caatgtccat atttaaataa
     1441 catttgtttc tttttgataa atttgctttt tctacataaa aagacgtaaa tgaagaatat
     1501 tcttctccat tcacgcctta aattgatggt cgtttgaata actatttaac aagtaattct
     1561 ctttggatat tatccgttat ggattatact tcctgtgtcg tcggacggat aatcatgtta
     1621 ctgacagaca tccggtctgg cgtttctatc gcaaatgcta cagcagaagc tatatcttcg
     1681 gaggtcaatc cccattcttt ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg
     1741 atggtttgat ataattcagt ttgaacagct cctggagaga tgatcgtaga tttgatgtta
     1801 ttttgccgtt gctcttgacg taatccttcc atgattgcac ggacagcaaa ttttgttccg
     1861 caataaacgg cggaatcagg ataaacaaca tgtcccgcca cagaatcagt tgaaatgata
     1921 tgcccagatt tttgtttttc catgattgga agtacggcgg aaattccatt taacacaccc
     1981 atgatgttga tatcaagcat attttgccat tcatcacggt gaccttcaac taatggagcg
     2041 gttggcatga ttcctgcatt attgtacaat acatcgattc gaccgaactt ttccatcgct
     2101 aaatcgataa ctgcttggac ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc
     2161 tcagcttctg gtaaagattc ttttattgct tttaggcgat cttctcggcg tgcagctata
     2221 actagtttag cccctttttc tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa
     2281 gcgcccatga tgacaatcac tttttctgat aaatcattca ttctattctc ctcctgtttt
     2341 gaaaaatctc ttgcttatac tcactatctc ttatttcatc ttactgttca aataatccgt
     2401 tcgacaggtt tgacgatttt ggcagggatt cctgcaacga tggtattgtc tgggacatct
     2461 ttcgtaacag ttgcattcgc tgcaacaatg gcattctcgc caatcgttac gcctggcaga
     2521 atagttgctc ctgctccgat ccaagcattt ttcttgatgt ggattggctt tactgacaag
     2581 cctcttcgtt cttttggatc ggttagatgg ttcaccgtga tgaggttgac acgaggacca
     2641 atcaatactg aatcttctaa acagatgcca cctaagtcaa cgaacatcac ttgacgattg
     2701 ataaagacat ttttccctat actaatatgt ttgccgaaat ctgtataaaa aggcagcaga
     2761 acagtcaccg tatcatctac tttgtcacta atgatatttt ctagccgttt gcggatatct
     2821 gctggttcat ggtacccagt attcagcgct gctaaaggct cttcgttttc agcttggatc
     2881 tgatggatca cttcaaagag cggatccgtt actcgaatct ctttattgac gatttgtttt
     2941 tcgatgaggc ttgtcatttt gctccctcct tttatcctta ctttataacg gacggcttat
     3001 catgtctaat acttataaaa catacttaac tattcctttt aactatactt agtggtatgc
     3061 ttttttaaaa acaggaggga aaaaatggat attcgtatac tgacgtattt tttagcagta
     3121 gctagagaaa aaaatatcag ccgagcagca aaatctctgc atatcacgca acctactctc
     3181 tcaaaacaac tgaaagattt agaagaagaa cttggtgtga cacttttcac tcggggctcc
     3241 cgagagatac agttgacgga ttctggtcga tatctttatt caaaaggaaa agaaatcttg
     3301 tcgttggtag aaaaaacggc gaacaattta acaaatgacg caatcatctc tggagaaatc
     3361 tacattggcg gaggcgaaac taaagcaatc tctttcattt ctgatagcct tcattcgttg
     3421 atcaaggcgc atcctgatat ccagtttcat ttatatagcg ggaatgcaga tgatatcata
     3481 gacaaactgg atagcggatt acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa
     3541 aaataccagt catttcgact gccagcccaa gatcgttggg gacttttgat acatcgaaat
     3601 catcctttag caaagcaaac cgtcatccat ccgcaggaac tgaaaactgt tcctttgatc
     3661 atttcccggc aaacgtcagt ggacagtcaa ctagctgaat ggctaggttt cagcatagat
     3721 cagttatcga tcgtcggaac atataactta ttgtacaatg cttctttgat ggtcgataac
     3781 gggacttttg gtgcattagc tctagatggg atcatcaata cttatgggac ggaaaaaata
     3841 ttcatcccac tcgatccgcc attacaagct tcactgaatc tcatttggaa aaaagaccag
     3901 cctctttctc ctgctgctcg taaattttta gatgtcctaa cggagaaata cagttaagta
     3961 gagttttttt ccttcgcttt cagcttttta acaacgaata agataagcaa aagatccaac
     4021 gattaaataa cataagtttc ttaatttatt tcttcctttt atttctgata attccagtaa
     4081 attactataa ttataaaaaa agaattgaca aaacataact gtctatataa aattgattac
     4141 gttaacaata attcgtttca tgtgactaga tcatactagg catgaaaaat ctctgaggaa
     4201 catggtttcc ctaggaggtt tttcgtgtct atttttgttt ggccgcaatc aactggtcta
     4261 gcaaacgtga aaaccaaatc ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta
     4321 atcaaaatgc agtattggta gatgacggtg gacaggaaaa agtagctatt ggaaaaggga
     4381 ttggatttga cagaaaaaga aatgatttga tttttgatcg tgatgtcgaa cggatatttg
     4441 tcatggaacc agaaggtcaa atcaagttgc aaaatttact taatcagatt gatgaacgct
     4501 ttttatttgc agcagaaacg atcattgatc atgcggaaac tgttttgatg gaaaagttga
     4561 atgaacatgt attgatcgct ttgaccgacc acattgcttt ttctgcggag aacattaata
     4621 acggtatcgt catccgcaac aaacttttaa gagaaatcga agtgctttac agtgatgaat
     4681 tcagtattgc tcaatgggca gtagagtatc tgacaaaaga gcttgggatt ccatatactt
     4741 atgacgaagc aggatatatc gcaatccata ttcacagcgg acgaagcggt cggaacaata
     4801 accatcgcag tatccgtgaa gtgacgattg tatctgacat catccattta gtggaatcag
     4861 aattggaaac agatatccat tcagaaccat tttcactgaa ttattcaaga ctggtgaatc
     4921 atttacgact gcttctacaa cgaacccatg cgcaacaata tgctgtttta gacacggaaa
     4981 tcgtagaaat ggtcaaacga aaatacccag aaagctataa gatttctaaa aaaatccgtg
     5041 tgttgctgac caaagaatac cagctcgcga tcacgaaaga agaactgggt tacttggcca
     5101 tccatatcga gcgtttaaga agcgcaatcg tacaaacaaa tgttaacaat catgaggagg
     5161 aaaaaaattg aaagcatatc tacaacgaat gggacgttcc cttcaacttc cagttgcggt
     5221 cttacctgct gctgctttac tagtagggat cggaaactgg tgggcatctt atagtaacga
     5281 cattgtcgct cattttttac aagctggcgg taacgcagtt ttaggtcaat tacctttgct
     5341 gtttgccgtt gggttagcac tagggatgtc agtggacaaa tcaggtgctg ctgccttggc
     5401 aggtttggtc gcatttgaag ttccagtaaa tgttttaaaa actgattcag tagctacttt
     5461 attgaatatc aaaccagaat cagttgatcc atcttttgct cagatcagta acgtatttat
     5521 tggtattatt gccggattga ttgcggcagc tctttataat cgtttccacg aagcaaaatt
     5581 accgatggct ttatctttct ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt
     5641 catgttgctg atcagtgcag tgctactgct cgtatggcct ccggtttact ctgctttagt
     5701 ttcttttgct aaattcattt tgagtttagg ctggggtggt gcaggtcttt atggcttctt
     5761 caaccgttta ctgatcccta caggattgca tcaagcaatg aactccgtgt tctggtttga
     5821 cgtagcagga atcaatgata ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac
     5881 aggaatgtat caagctggat ttttcccaat catgatgttc gggcttccag caggagcata
     5941 tgctatttac agaaatgcac gtccagaaaa caaagcaaaa acagcttcct tgatgctagc
     6001 agcagctttt gcttcattct ttactggtgt gacagaacca cttgaatttt catttatgtt
     6061 cgttgcttgg ccattatatg tcttgcatgc aattttcact gggatttctt tagcagtgag
     6121 tgcattcttc cattggacag caggatttgc ctttagtgcc ggatttgtcg attacttcct
     6181 atctttgaaa aatccgattg cgaatcagcc attgatgttg atcgtccaag gattagtaac
     6241 agcagtgatt tactactttg gctttaactt tgctatcaaa aaattccatc tgatgactcc
     6301 tggccgcgag gaagcagatc tttcagatga cgatactgca acgactaatt cagacaataa
     6361 atatgcggca caagcaagca aaatctacgc tgcattaggc ggagccgaca atgtaacttc
     6421 gatcgataac tgtactacac gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca
     6481 aaataaaatc aaagccactg gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca
     6541 agtcatcgta ggtacagaag tacaattcgt tgcagatgag atggcaaaac tacatggtgg
     6601 tgctgcagca cgacctgcac aaacaaacac cgtggttaaa acagaaacat ttgccggtga
     6661 aacgatcgaa caagatattt acgctatcgc aaatgggaaa ctgattccaa tcactgaagt
     6721 ttcagatgat gtattcgcgg aaaaaatgat gggtgacggc tatgctgtat taccagaaaa
     6781 tggagaaatc ttctctccaa tcgctggtac gatcacaaac attttcccaa cgaaacatgc
     6841 tgtcggaatc caaacagatg ctggtatcga agtcttgctt catatgggca tcaacactgt
     6901 tgacttaaaa ggcgaaccat tcactttata tgtagaagaa ggtcaaaaag tcgcaagagg
     6961 tcaattgatt gctttagttg atcttgccgc tattcaatct gcaggcaaaa acacggatat
     7021 ggtcgttgta ttcacaaatg gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga
     7081 tgtaaaagca aatgataaaa tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc
     7141 cagacttttt tatcgataaa caaacaggag ctgagacaaa aatcttgtct cagctccttt
     7201 ttccgaataa aaacggcgtt ctcctgccaa ctccgcggta ataagtccag ctaaacgaaa
     7261 catatgaaga gctatttttt atttgactct tcttattact tgatgcagaa cagctttttc
     7321 acaaaatttt tcttcatact caaaactaat cggctttttc acaacctctt tatacattat
     7381 tcctgatcca atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact
     7441 ttttctaaat catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct
     7501 aaaaacatgt cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct
     7561 gcatcacttg cttcaataat ttctaactct ttacctgtcg cacgctctaa tgccttggct
     7621 aattcttcat agtcagttag attgccggat aattctaaga tttctggaga gtcactttct
     7681 actagtactt ttgctgcagc ttctgcatat tcacgtttca atgcccaacc tgtttttcca
     7741 tttcctgcag cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca
     7801 ttctctacat accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt
     7861 ttttcagtga attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa
     7921 cttgtataag cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt
     7981 tcggcttgac gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac
     8041 gcttctttca ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct
     8101 ttcagtgctt ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc
     8161 atctttttta agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa
     8221 taatgcatat ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta
     8281 taaattacac ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg
     8341 atgacaactg atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat
     8401 tgaaatgttt caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac
     8461 aaatgcagca aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac
     8521 aaaaaccgga aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga
     8581 tgtctatcgc agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc
     8641 aaattgcgtc gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca
     8701 gaaaaaggca gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc
     8761 cgttttggaa aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt
     8821 ctctctactg tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc
     8881 gggggacaaa agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga
     8941 agtcatgacg tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa
     9001 atgcaaaata tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta
     9061 ctttcacgac ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata
     9121 tatttcccgt tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa
     9181 tcttctactt ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct
     9241 tcaggcgatt ctgctaaagc gcttacaaca agatacgttc cttttgggaa tgggatgatt
     9301 cgcgtagtgt ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg
     9361 ttatagggtt cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct
     9421 attgttccat taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca
     9481 aaatccgtat tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt
     9541 tcttcgatcc tatactttgc catttctccc accctttcta ttatattaag ttcatcttac
     9601 ataaaattaa agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt
     9661 aactattttt ttgctttcac tgcaatccaa atctcactgt aaacattact gaaatcctct
     9721 ccccattttg taaaagagat ctcgggagct tcgactaacc cataatctga agaaggaagc
     9781 cattctgcat agattttccc ccaagtatct tgtaaagttt ggggaaatgg ccctttatta
     9841 ggaaaaatgg cccaagtgtg ttcttctata gcaagcattt ctaaatcagc gtacggattt
     9901 tcttttgtcg tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc caagcggtct
     9961 ccatcaaatc cataagaaac gttgattact tgatttggat ataaatctcc taacttatgc
    10021 atctcttccc tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc ttgattgact
    10081 ccttcaaatt tgacaggaac ttttttcgcc acacctgcca cataaaactg ttctttcttt
    10141 tcgatacgaa attccatcga ttttcctcct ctgacatcaa taaaaaatgt caagcgtgga
    10201 aaagattttt gtatgccgta tctactgatt tctgaaggca gatatcctga ccaatcacga
    10261 aaagctcttg aaaaaccttc gactgtttca tatccatatc taaacgccgt ttctgtcact
    10321 cttgatccat ctagaagatc gcgattagcc aaagccaatc tacggttttt gatatagtcc
    10381 attagcgaca tccctgcaat aaacgtaaag atgcgtttta attgatattc agagattcct
    10441 gtattttcgg ctattatttt ataggaaagt ccttctgtca aatgttcttc gatgtaatcg
    10501 atcaattggt tcagatcttt aagcatattt atcttcctcc ttgattgcct ttttatcata
    10561 ttatcaaaga aagtcaatta cttgatgaat caggaaccaa tttagtcaga tgtagaaaat
    10621 gttgtatgga tctaaagaaa attctcaagc agcgtttcgt aacggataat tggtattatc
    10681 tgcctatcta tgttttacct ttagaaaaat aaattttggg gaagacagat tctaagtaaa
    10741 caaagcggta aaaatatctt ttattccctt agttaatccg cttactacct actgtacaaa
    10801 gcaaattgtt gttcaaactg ctgatcagaa cgctgcttgg atttttcttt gcgatacatc
    10861 atacctcttt tatcaattgg aacaattatt tatttctaac aaaatcactt catttttgtc
    10921 agataaataa aaaacaaaaa aagttccatc tttaaaacac gctattcttt tactttttct
    10981 cacattctct aagaaaagaa aaggagaatg aatttttgac aattcgagca aaagtgatta
    11041 tcaaatatgt aaaatcagct acttttttct taaacgtttc tttatactaa atataaataa
    11101 gtgtttcatg aaaggagaac tagatgaata attggcttga gtattttcct gaaaacgttt
    11161 tggaacgcgg ttacagctat catctgcacg gttttgtccg tcatttgaac tatacaagca
    11221 agtacttgtc cgcaacagtt tctggcacgg aagattataa ggtagtgatc acttgggatg
    11281 aaaagaccaa catgacttgt gattgtctat atgccataga aggaaaaaaa tgcaaacaca
    11341 tggctgctgt cttattcgct tacgaagaac gtccaataaa aaaatccaac tactcacttt
    11401 ccgaactctc gtcccttgtt tcctccgcta gttcctctct tgtacgggag cttttaacag
    11461 agattcttat agaacatccg cactttatcg aacgcttcaa agttaagatg ccatttcatg
    11521 caataaatta ttcagataaa ttaacaacta tcatccataa atacgatcat attatcaaga
    11581 aaaacaaaaa caggaaaaca gcgaagttca tcatggagat gcgaaagttc atccaagaag
    11641 cagttgaatc gcttattcag caaaatgcct atcttcccgc attcgaattg atcaatgaag
    11701 tgattgctac tctcgaaaca ttctattggg agcctgagga tgaacgaacc cttcttctga
    11761 tagaggactg ttattattta tggaaagaat tattagcaga agcacctcat gcagaaaaaa
    11821 ggcaaatgtt ctcttggttc gtctgccaag tcgatcatac tgacgcctct tattcaaaac
    11881 gctactctat aaaaatcctg aaagaagact ttcgggaaaa agaattttct aaccaaaaga
    11941 aaaaaataga taaaaaacta aagaaacggt aaagaaagac gatttcaatg aaaaatagca
    12001 agtaaacgat aacaaccatc ctaaatccac aagaaataca gcagaccttg ctttaaaaac
    12061 acgaatcatg caaaggatga agtaaagaag acttaacgcg tacccaatat tagtcatttg
    12121 actattaggt acgcgttttg ttttataagt catgacaaat ttccttcgtt gctggataga
    12181 ttttccggca ttgttgatag attttatgat acttttctac attttgtaag agaggttccg
    12241 ctgcatctgt atattggata aaattctgac aacaactttc aggtgtgtcg aaccacccta
    12301 accctagtgc agcaatcatt gttgcgccta atgcaggtcc ttcctccata cttaaacaat
    12361 agaccgttgc atcgaaaata tcagcttgga tttgtagcca tgcaagattt ttcgcgcctc
    12421 ctcccaccga aacaatacgt gtaaatctgc gacccgctag ttctttacgt ttcaatccca
    12481 ccattgacaa atgctcatta aaaatttaga atattcacaa gtttaacgac aattatgaaa
    12541 atttatgtcg tgctaaagga gttttgttat tggatgaaaa gttagatata aaaactgcga
    12601 tcaaagacac gttacctacc gttttcggtt atatcggtat tggacttgca tttggtatcg
    12661 ttgggaaagc tgccggattt catccactag tcgtcacgtt gatgtccctg ctggtctatg
    12721 ctggttctgc ccaatttatc acagtcagca tgcttgctag tcacagccca ttgctttcca
    12781 tcgttttctc gacctttcta gtcaattccc gaatgattct gatgagtatg acgattgctc
    12841 cttatttcaa gaaaaatcgg ctgcttcaaa atctgctgat tggcacattg ctgacagatg
    12901 aaagtttcgc actgggaatg aataaattga actatactgg acaaaagtta aactttcgtt
    12961 ggatgaatac agctaattgg atttcttatt tgacgtgggt gggttcttct cttgttggcg
    13021 cacttttagg gaattttatt actgatccga aaaagtttgg tttggatttc gccatcgtcg
    13081 ctatgtttat cgggttactt tatcttcaag tcatttctga tagaaacacc tctaaacgat
    13141 tacagttgat cctgattggt ctcactttga ttctggtata tgtcggactg atttttatcc
    13201 caagcaactt agtgattgta gttgtaacac tgattgggtg cggattgggg gtgtggatca
    13261 aacatgcctt cttttgagtt tacattgctg actattttag gttgtacttt agcaacttgg
    13321 ctttctaggg tcctaccatt tgtcttactg aaaaaattcg atttaccgca accgttgctt
    13381 gagtatttaa gttttgtccc gatcgtcatt atgtctgctt tatggttcag cagtctattt
    13441 acccaaaata tcggacatct gccgcagatc aatctcgaaa atgctttggc ttcagttcct
    13501 acacttttag ctgctattct ttcaaaaagc ttattggtga tcgtcttggc aggaattctt
    13561 tccctttcgt tgattcgctt attataaata aaaaaacaag agcttatggc aaattactgt
    13621 catgcgctct tgtttttatc aaagtcaaat aagaaaaaaa tcggcgtatt cttgcttatc
    13681 cgttgaacgg accactaaca acgacttttc ctaacatatg tcctgtttct acgtctttgg
    13741 ttgcttgttt caagttcttc gcattcatcc catctgaata gaccttgctc aatgtactgc
    13801 ggataatccc ttcatttgct aaagcagcaa tgtgcgctaa agcttctcct tgcgtttcga
    13861 tttcatagtt atagtctgtt ttagcgaaca tatactccca gtcgaagctg atagattggt
    13921 ttttccaaac acctatatct aacggctctt tgatccctac gatcgttccc acatgtccaa
    13981 atggtcggac aaggtgcttg atttgatcaa agtagcgggt aatatcgaat aatacagcga
    14041 catactctac ttgatcgatt cctaattcac gaaaactttt acttaagtcg tgatgataat
    14101 cgattggata gtctacacct gtttttttca gccattcaaa attcttcgga cttgcagtcg
    14161 cgtagacttc taggccagcc caatgcgcta gttgattcaa aatcgaaccg acgcccccgg
    14221 atccgttgat cactaagatt tttttcccgc gattggcatt ttcttctgga gttagaccaa
    14281 atttttcaaa taacaattca taagcggtca atgaggtcaa cggcaaggct gcagcttctt
    14341 catctgaaag attttttggt gcaagagcga caatacgttc gtcaactagt tggtattctt
    14401 gattgcttcc tgctcttgtt gttgtacctg cgtaaaaaac gcgatcgccg acagaaaatt
    14461 tttgcacttc gtcacctaca gcaacaactt tcccgactcc atcaaaacct aagatacgta
    14521 aagcatttct tatcccgtta tcttggcgaa gtttcgtatc gacaggatta acagatactg
    14581 cgttgacttt taccagaaga tcccgtgaac tgggtacagg tatctgttct ttttcatcaa
    14641 taaagctttt tggatcatcg atcggcaacc cttcataaaa accgactacc ttcattaatt
    14701 ccttttctga catacgaatt cctccttagc ttcatcaagt tggagcatcc atacagtgtt
    14761 catttcaata tatatagtat gctatttgtt ccaccttttt tattcatcat aattattctc
    14821 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
    14881 gtcacaacct cttataaacg gtgttctcct accaactctt cggtcacaag tcaaaa
//
LOCUS       DBNIBO010000058        14886 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.062, whole genome shotgun sequence.
ACCESSION   DBNIBO010000058 DBNIBO010000000
VERSION     DBNIBO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.062"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(5..823)
                     /gene="purR"
                     /locus_tag="AC1Y77_001496"
     CDS             complement(5..823)
                     /gene="purR"
                     /locus_tag="AC1Y77_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HIQ1575075.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(990..1814)
                     /locus_tag="AC1Y77_001497"
     CDS             complement(990..1814)
                     /locus_tag="AC1Y77_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIQ1575076.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1787..2467)
                     /locus_tag="AC1Y77_001498"
     CDS             complement(1787..2467)
                     /locus_tag="AC1Y77_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575077.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2490..3428)
                     /locus_tag="AC1Y77_001499"
     CDS             complement(2490..3428)
                     /locus_tag="AC1Y77_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIQ1575078.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3673..4524)
                     /gene="ispE"
                     /locus_tag="AC1Y77_001500"
     CDS             complement(3673..4524)
                     /gene="ispE"
                     /locus_tag="AC1Y77_001500"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIQ1575079.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="AC1Y77_001501"
     CDS             complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="AC1Y77_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HIQ1575080.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7392..8198
                     /gene="proC"
                     /locus_tag="AC1Y77_001502"
     CDS             7392..8198
                     /gene="proC"
                     /locus_tag="AC1Y77_001502"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIQ1575081.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(8277..9833)
                     /gene="rny"
                     /locus_tag="AC1Y77_001503"
     CDS             complement(8277..9833)
                     /gene="rny"
                     /locus_tag="AC1Y77_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIQ1575082.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10244..11293)
                     /gene="recA"
                     /locus_tag="AC1Y77_001504"
     CDS             complement(10244..11293)
                     /gene="recA"
                     /locus_tag="AC1Y77_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIQ1575083.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(11402..12643)
                     /locus_tag="AC1Y77_001505"
     CDS             complement(11402..12643)
                     /locus_tag="AC1Y77_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIQ1575084.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="AC1Y77_001506"
     CDS             complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="AC1Y77_001506"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIQ1575085.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(13670..14593)
                     /locus_tag="AC1Y77_001507"
     CDS             complement(13670..14593)
                     /locus_tag="AC1Y77_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIQ1575086.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4268 a         3306 c         2591 g         4721 t
ORIGIN      
        1 cctcttattc tgaaaaataa tttcctggca caaccgtaat atttttcgtt tgagtatcta
       61 catctttgac ataaagcagt gacgtataat catcgatggc gcgatgaccg ttgaattttg
      121 attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa ttcttcaatc aagctcttca
      181 ttccgttgac tgttccgccg cctttcatga aatcatccac tacaaggaca cgtgaaccac
      241 gtttcaaact tctcttagac agttccatct tttcaattct ttcagaagat ccagacacat
      301 agttgacact tactgttgat ccttctgtta tttttgagtc ccggcgtaca ataacgaatg
      361 gcgcattcag ataataggac acagcttggg caataggtac gccttttgtg gctacagtca
      421 ttacagcatc gattttttca ccaaggtatt tagaagcaat gattcttcca acttgacgca
      481 gcagttcagg ttctcctaac aaatcagaga gataaacata tcctcctgga agcagacggt
      541 cttgttctga tagacgttct gctagagatt caatatattt tttcgcctct tcatatgaaa
      601 tttcagggat gaacaataca ccacctgcag cacccgggat tgtttccaag gttccatacc
      661 ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc actgatagaa gatttagcag
      721 attcataacg ttcggcaaag cttgttaagg aaatcaattc atgcgggtga ttcaataaat
      781 actgtgtcat gtcaactaga cgttcactgc gacgtacttt cacctattcc aactcctttt
      841 aagaaatttt atcgttttac attatataag tttttcttca aaaattcgag cattttatga
      901 aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa aaaatttttc
      961 gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa ggcaaccaat
     1021 attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt gattgttgct
     1081 cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc tagaaatcct
     1141 acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat accgattgct
     1201 gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat catcacagca
     1261 atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg tagcccatcc
     1321 acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa taaaaagaac
     1381 aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca tgtaatggtc
     1441 acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc gctcaaattc
     1501 atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga gatttctgaa
     1561 taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac aacgactagc
     1621 atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc tgctaaagag
     1681 acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa cacacctagc
     1741 atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat gaattcatat
     1801 gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg tccgcatact
     1861 gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg acatgcgcac
     1921 tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct gttgttggtt
     1981 catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg attcgctgct
     2041 tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc cctacagctt
     2101 ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac cagcggcctc
     2161 ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct gcattgaaag
     2221 atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga tttttctcag
     2281 caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc accaaggttg
     2341 acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct acatgataag
     2401 agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat atttcgatat
     2461 aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa agctttcaga
     2521 tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt agtcaagctt
     2581 tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt ctcagctacc
     2641 ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt cacaaattct
     2701 tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc cgaaatcgct
     2761 acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg agtgacaaat
     2821 gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc ttcaagcttt
     2881 ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata tttttcgcct
     2941 aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag ccaaacatgt
     3001 ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc gtctgcttct
     3061 tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg tttgctatcc
     3121 actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata cacgaaaaca
     3181 tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg cggctccgtt
     3241 ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt tgtaaaatca
     3301 tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc ttccttgttc
     3361 gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt caaaagatat
     3421 tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt attaacgtga
     3481 actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc tgatttaagc
     3541 atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct agccatttca
     3601 ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt
     3661 acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa
     3721 cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc
     3781 gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg
     3841 gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat
     3901 ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat
     3961 ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac
     4021 caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc
     4081 gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat
     4141 ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc
     4201 agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac
     4261 aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata
     4321 aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc
     4381 ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc
     4441 caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc
     4501 tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat
     4561 tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc
     4621 gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta
     4681 taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag
     4741 actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg
     4801 tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa
     4861 aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt
     4921 ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc
     4981 gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa
     5041 gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc
     5101 aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc
     5161 aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc
     5221 atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc
     5281 tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag
     5341 atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac
     5401 tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt
     5461 cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac
     5521 aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg
     5581 gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta
     5641 tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc
     5701 ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag
     5761 ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc
     5821 gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa
     5881 caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc
     5941 ttttctggat aaccacacag ctaacagaat caagacatat accaatatca actgccaaga
     6001 taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa
     6061 tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa
     6121 tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg
     6181 actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata
     6241 gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc
     6301 taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa
     6361 aggtagaaca taataaaaca accgataata taatagccaa actactgcta catcttgccg
     6421 aacaggcaat tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg
     6481 gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat
     6541 cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca
     6601 ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa
     6661 tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa
     6721 aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa
     6781 tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt
     6841 ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc
     6901 taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag
     6961 aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca
     7021 atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt
     7081 catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat
     7141 atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat
     7201 cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca
     7261 ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc
     7321 cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag
     7381 gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg
     7441 atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt
     7501 ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg
     7561 aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag
     7621 tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa
     7681 aatcattaac agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga
     7741 tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg
     7801 ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc
     7861 ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg
     7921 tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga
     7981 cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg
     8041 ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag
     8101 gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga
     8161 cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc
     8221 cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat
     8281 ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa
     8341 tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc
     8401 gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt
     8461 gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag
     8521 ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct
     8581 acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg
     8641 attactactg ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat
     8701 ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct
     8761 aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc
     8821 gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg
     8881 attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg
     8941 cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga
     9001 tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt
     9061 ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca
     9121 gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt
     9181 tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag
     9241 cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc
     9301 tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc
     9361 atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt
     9421 tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa
     9481 ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta
     9541 cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta
     9601 ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct
     9661 aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat gattccagca
     9721 gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct
     9781 aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc
     9841 acctccatac tatctttttc atttttatca tctatacagc ttctatgata aattattcta
     9901 aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta
     9961 atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca
    10021 ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga
    10081 gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca
    10141 ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag
    10201 agtacctact cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag
    10261 gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag
    10321 ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt
    10381 tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt
    10441 tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta
    10501 aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga
    10561 ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac
    10621 gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg
    10681 gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt
    10741 ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta
    10801 acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa
    10861 ctgaatcgac taccacgata tctacagccc cacttgatac taaagcatca gcgatctcta
    10921 gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt
    10981 tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat
    11041 ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc
    11101 catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg
    11161 caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa
    11221 tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac
    11281 gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat
    11341 ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt
    11401 atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc
    11461 tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg
    11521 aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc
    11581 cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct
    11641 ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa
    11701 aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac
    11761 cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta
    11821 cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc
    11881 cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag
    11941 cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg
    12001 gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag
    12061 tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt
    12121 cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact
    12181 cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta
    12241 aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca
    12301 gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc
    12361 cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt
    12421 catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta
    12481 gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt
    12541 cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc
    12601 ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta
    12661 tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg agctgtgaca
    12721 aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc
    12781 ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc
    12841 agagttagac ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa
    12901 cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc
    12961 ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa
    13021 cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc
    13081 catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca
    13141 gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc
    13201 agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag
    13261 gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat
    13321 aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca
    13381 atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt
    13441 taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca
    13501 gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt
    13561 attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc
    13621 ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt
    13681 attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta
    13741 cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat
    13801 ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat
    13861 aaacataggc attgtctact agcacaccta accatacacg attgacagca gtaaattcaa
    13921 gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc
    13981 ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag
    14041 actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt
    14101 taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat
    14161 agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg
    14221 tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg
    14281 tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat
    14341 cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca
    14401 cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt
    14461 tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt
    14521 gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga
    14581 ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt
    14641 catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa
    14701 gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag
    14761 ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat
    14821 tagctcaaaa accggcaaaa tataaggaga tatttttccg tatgtttctc taatactcag
    14881 gtcaaa
//
LOCUS       DBNIBO010000059        14832 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.063, whole genome shotgun sequence.
ACCESSION   DBNIBO010000059 DBNIBO010000000
VERSION     DBNIBO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.063"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(23..1717)
                     /locus_tag="AC1Y77_001508"
     CDS             complement(23..1717)
                     /locus_tag="AC1Y77_001508"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIQ1575087.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(1774..1902)
                     /locus_tag="AC1Y77_001509"
     CDS             complement(1774..1902)
                     /locus_tag="AC1Y77_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HIQ1575088.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            1901..2149
                     /locus_tag="AC1Y77_001510"
     CDS             1901..2149
                     /locus_tag="AC1Y77_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575089.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2341..2706
                     /locus_tag="AC1Y77_001511"
     CDS             2341..2706
                     /locus_tag="AC1Y77_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIQ1575090.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2703..3068
                     /locus_tag="AC1Y77_001512"
     CDS             2703..3068
                     /locus_tag="AC1Y77_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIQ1575091.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(3160..4587)
                     /locus_tag="AC1Y77_001513"
     CDS             complement(3160..4587)
                     /locus_tag="AC1Y77_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575092.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            5057..6892
                     /gene="tdc"
                     /locus_tag="AC1Y77_001514"
     CDS             5057..6892
                     /gene="tdc"
                     /locus_tag="AC1Y77_001514"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HIQ1575093.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            7197..8675
                     /locus_tag="AC1Y77_001515"
     CDS             7197..8675
                     /locus_tag="AC1Y77_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1575094.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            8641..9972
                     /locus_tag="AC1Y77_001516"
     CDS             8641..9972
                     /locus_tag="AC1Y77_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1575095.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            10086..12638
                     /locus_tag="AC1Y77_001517"
     CDS             10086..12638
                     /locus_tag="AC1Y77_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIQ1575096.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(12645..14084)
                     /locus_tag="AC1Y77_001518"
     CDS             complement(12645..14084)
                     /locus_tag="AC1Y77_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575097.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            14059..14241
                     /locus_tag="AC1Y77_001519"
     CDS             14059..14241
                     /locus_tag="AC1Y77_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575098.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            14430..14792
                     /locus_tag="AC1Y77_001520"
     CDS             14430..14792
                     /locus_tag="AC1Y77_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIQ1575099.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         4663 a         2483 c         3035 g         4651 t
ORIGIN      
        1 cagctttttc acaacctctt gattattcaa acattttttt tgcggcacga gctagagtag
       61 gaatatcttt gtcgtaacgc ggtgttttga caagacggga cataggaata cccgcaaaat
      121 ggaaagcagc gatttctcca gaacgtacag cactttgttc agtaaagatc atctgataag
      181 gctgctcgac aaattctcct gtaaaggcca gattggtcga atgtttagga atgacttttg
      241 gtcgatcgct tttggcacga ttattgaaca acgcggaagc ataaggcata tagacaggga
      301 tattatttat tacgctgtcg aaaatttctg tttctttttc acgaatattc actggtccag
      361 gatctacttt tgaaagttgt ccaatcagtt cttctagcat ttcttttcca ttcattttaa
      421 tatactgttt atccacaaat tcgccacgtc gtcttggata aaggaaataa ccccaaataa
      481 ctgtttcatt tggtttctga gacgtaaaat gcggttgatg atgcacgacg atagacatat
      541 tgacatcttt ttggcctaac ggcgtgatcg gtgtggttga aataaatgaa ttcaaggcat
      601 ttcctggttc ttgagtcgtg atacgggtga tttcattcaa caacaaatgg tttttcgtcg
      661 tcagggtaaa gctgacccat tcgcttgcat ctcgatcagc aaagaatttg tcaggatttc
      721 ccaagttgta gaaacgttca ctggcttttt tccagagact agaagctgca ccataatcca
      781 tattttctac agctggtgtt tcgtaatctc ctaaagtagc tgaatctgta atggacccat
      841 tagtgaaaat aactgctgta tcttcatcga ccacaacgta ttcttcttct tgagtcagtg
      901 tattggtaat tttcaaacca gtcacagtga tttcatcttg catcggtgtt tctttgaatt
      961 cccagtcagt gacacggcgg ttcaaaatga ttttgcatcc ttgttctttc aagtactcga
     1021 tcaaaggaag cataatactt tcgtattgat tgtaacgtgt tcgattgacg cccactagat
     1081 gttcgatttg agtgaattca taaatcattt gatgcatata gcgacgcaat tcttgagcag
     1141 aactacgtgt tctgaaagca aatgttgttt cccacataaa ccagaagttt gtttcaaaaa
     1201 agtgtggatc atctttgaaa tattcttcga tagtgatatt gtctagtttt tcttcttctg
     1261 aatcaggcat agcaatcagt ttagtcagaa gaatacggtc tttattattg aatcccagtt
     1321 ttccgccatc aatgattcct ttgccacctt ccatcaaacg tgctttgtca aaggttcggt
     1381 gttttgcatc aaaatctctc gtatcttctt cagcagtcat ccctggctca gtaacagaag
     1441 gtattcgact taacagatcc atcaaatcta cataggtacg gtaattaagc atgcgaccgc
     1501 ctcgagcaat atagcctttt tggttttcta atggatggtt tttattccaa tattcatcca
     1561 ctacagtgtc ggtcggtgca ccatcatttg aaccgtgatc atccaatgaa taaaaggtga
     1621 tctgatcccc tttccatttg ccttcttgaa tcaaataaac agccgctgcc atattggcaa
     1681 taccggcacc aatcattatt gctttacttt ttctcattgt gattcctcca atgtcttcca
     1741 agtatgtccg tagtgattca actaaatagg atgctagatt gattcgaatt catcatataa
     1801 atgactgtca tcttcgaata gtcctgtctt ttttcctaga tatgcagcgc caagggcaag
     1861 caaacctaaa ccgatgccga ctttgattaa ttttttactc atgaaaaacg cctccttgtt
     1921 ttctctttct atatccaagg atagttcgtt tttattagaa tgccaaatga taggacgaga
     1981 agaaaaagga aattttgaca aaaaagaaga agtgtctgat tttcctgtaa ataaaaatag
     2041 ggtaggtaag aagaaaagat ttgaggaggt gtatggttct atttttagaa atttgggtac
     2101 aaaaaaagca ccgagacagt gcttctttga aaagctgatg acggactaag ccttatttta
     2161 acttgctacg ttgttttgga tacttccacg atttatggat ggagggagaa aaatgggaga
     2221 gtgaaaaaga aaaatttaag cgaaccgtta tcattagaac aacttttaaa tagacgaaca
     2281 aggtaacttt cttttacaat aaaagaatag gaacaaatta aaagaaagta ggtagcgtat
     2341 atgtttcgct caaatttaat tgaatttcta gaatggatca ttgctatttt aaatatttgt
     2401 tctatcggta tcttaattgt cgggatcgtt cttgttctat tcaaactttt tgattggtct
     2461 catgtcaaga aaaactatgc agaacgtaat actcgtaatg cagatacaag aaaactgcta
     2521 gcaagttata ttttgttaag cttggaagta ctagtcgttg ctgatatcat cgagtcagtt
     2581 atcaagccga cttggacaga tattttgaag cttgcactta ttattatcat tcgaacagtc
     2641 atttcttatt tcttgaatca ggaaatagaa gacaaagaaa aacaaagaag aggagaggta
     2701 ggatgacgac agagttgatg cagtttcttc ttccatattt cgaaaaactt gtactgggac
     2761 ttgatatctt tgctatctgt ttattggttt ggggtgtttg tctatcaatc aaggattttt
     2821 tgaaatattt atttgtccat acaaaagtgc aaagcgaccg agtcagcgag aacaatgcta
     2881 ttaaaaagat gttgggaggc tatatacttc taagtttgga aattcttgtt tcagctggga
     2941 tcatcgaatc gattatcaaa cctaccttgc aggatatttt ccaactagcg gcgttggtag
     3001 ttatccgaac catcatttct tattttttaa ataaagagat cggacccgta gatgtccaaa
     3061 ataagtgagg ttgtgagcct gacgtgtatc aagagattgt tacagaaatg ggcatcttca
     3121 acaaataaga ggctgtgaca tgatttttgt cacagcccct tatttgtttt gcacagctga
     3181 aatgatagct cgatcgatcg tattgatcga aataggaata atattttcat attggaccgc
     3241 atctggtgta tataatgtac gttcacataa tacccggttt gaggactgct gcgcatactt
     3301 ctttatatca gatatacttg aataaattgt tggtgcaact tgaaaatcga aagtagttac
     3361 agcttccaag gcttcccgat aaatcagata atgaacatcc gaatttgtga cgatatagat
     3421 agtcagatgt tcttttgtca gacggagaat tccgctcaat tcgatcacaa aagcccgcat
     3481 gcaattagga ttcaaccgta gatcataagg taactggctg gaccagctgg aaaaaattgt
     3541 ctggacttct ttatataaat ttgtctgttc ctcatttaaa agataaaatt tttctggcat
     3601 gaataattga tagttatccc aagccgaacg catcagatga atcagcgcac gttcgaacgg
     3661 ttggtttccg aataattctt gattgaagtg agattcaaag gtagcaacta gttcttttat
     3721 ttcgggagta ttgtcaataa acacttggtg tagctgttga aagtcttccg caatcaactg
     3781 ataagaatgg aaaccataat tacaaagatt gaatagtgtc aaaataaagg cgaattcttc
     3841 ttttttataa taggtttgga aatcagtgtt ttcccatgct gattcgacat aattgtaaat
     3901 aggacgtttt ttgatttctt cgataaattt tgaatctatt gtcacagcac acacttgctg
     3961 gcgtaggaaa ctgatagcaa aaccaatcga taagatttct ttattttctt tatcataaaa
     4021 tctgccacta ttttccgtta cggattcaat gaaacgatcg gcacgttcaa aaaacgattc
     4081 aggtagctcc caagaattaa aaccgcctaa acgatacgac acattaagaa gcagcagacg
     4141 tctttccatt tcagtgaatc gaggggagta gcggtcgatt tcgatcccgc agtcattaaa
     4201 ataagctaat acttgttttt ttaatgaata agctttgctg acagagatga attcacattc
     4261 agttaattga agataatcag gttcatcttc ttccaaataa agcagacaag tttttaaaaa
     4321 caacgaattt tgagacaatt gctgcatgat cttgcatcga ggtatatgtt ctgaaaaata
     4381 taaagtgtaa cggtcctttt cccgatgtgt ataagcgatc tgttcttcaa attctttttt
     4441 tatgttattg atatcattga gtaaagtggt tttgcaaaca tgcaggagat tggagagctt
     4501 ttctagattg acttctttat ttacatagaa ttgcccacat aaaaacagct gtcttaaaat
     4561 atttttctca atatattctt caatcatagt agagcctccg ccttgtattt ttttgcacaa
     4621 tatgggaaat attatgacat gttttctttt tgaaatcatc agttaatttc tgaaaaagca
     4681 tataaatgcc gttaaaaaag gttttttatg ttataaaatt tggaaaacag ataattctat
     4741 taaaaaataa attgcgaatt tttaacaata taaaaaaata aaaatatgtt tagtgaatga
     4801 aggacagtca ttttatttgt ttgttcgctt tttttaggtt atctatttta gaagactgat
     4861 ttattgaaaa attgttcgct tttttgcgtt gaattatcag aaaaatccga ctttatttat
     4921 ttttgtttgt aaaaatctac gtggatttga tgttataaaa aaaaaattct catttttggc
     4981 gctatagtga agaggtgtta cagataaaca catgtatctg caagacattg accaacaaaa
     5041 tatggaggga agaaaaatga aagatatgga tatcaaggcc gtctttatag gcgacaaagc
     5101 tgaaaatgga ccagtttata aaatgttgtt gaacaagatg gtagatgaac atcttggatg
     5161 gagagaaaat tatattcctt cagatatgcc tgcaattagc gaaggagata agctcactcc
     5221 agattatcta gccacccgcg accatatgat agaggtcttg gatgaagtca gtcagcgatt
     5281 acgcgcagga tcgatcccat ggcattcagc aggccgttac tggggacaaa tgaatgctga
     5341 aacattgatg cctgctttat tagcttacaa ctatgcgatg ctgtggaacc caaataatgt
     5401 tgcattggaa tcttctatgg caacttcgca aatggaagca gaagtaggac aggattttgc
     5461 ttcattattc aatatgacag acggatgggg acacattgca gcagatggtt ctattgctaa
     5521 tcttgaagga ttatggtacg cccgttgcat caaatcgatt cctttagctg tcaaggaagt
     5581 gctgcctgaa aaagtgaaaa aaatgtctga gtgggaactt ttgaatttat ctgttgaaga
     5641 aattttggaa atgaccgaaa gctttacgga cgaagaaatg gatgaggtca aagcagcttc
     5701 ttcccgtagc ggaaaaaata tccaaagatt agggaaatgg ctggtccctc aaacaaaaca
     5761 ctattcttgg atgaaggcgt tagatatttg cggagtcggc ttagatcaga tggttgctat
     5821 cccagttcaa gaggattatc gaatggatat taatgctttg gaaaaaacaa ttcgtgaatt
     5881 agctggacaa aaaatcccaa ttctaggtgt ggttgctgtc gtaggaacga cagaagaagg
     5941 tcaagtagac agtgtagata aaatcgtcca attaagagaa aggttaaaag atgaagggat
     6001 ctatttctat ttacatgttg atgccgcata tggcggctat gcgcgttcac tgtttctaaa
     6061 cgaagcagga gaatttgtgc cgtatgcttc cttagctgaa ttctttgaag aacatcatgt
     6121 tttccaccac tgcgtaacga ttgacaaaga ggtatatgaa gggttcagag ctatttcaga
     6181 agcggattct gtcacgatag atcctcataa gatgggctat gtcccttatg ctgctggagg
     6241 aatcgtaatc aaacataaaa atatgagaaa tatcatttct tatttcgcgc catatgtgtt
     6301 tgaaaaatca gtaaaggctc cagatatgtt aggagcttat attttggaag gatcaaaagc
     6361 tggagcaacc gccgccgcag tctggacagc acatcgtgta ttgccattga atgtgaccgg
     6421 atatggtcaa ttgattgggg cttcaatcga ggcagctcaa agattcagag aatttctcga
     6481 tcatctgact tttactgtta aaggaaaaac aatcgaagtc tatccattga atcatcctga
     6541 ttttaatatg gtcaattggg tattcaaaga acaaggctgt acagatttga acgctatcaa
     6601 tgaattaaat gaaaaaatgt tcgatcggtc ttcttatatg gatggcgatg tttatggtga
     6661 acggtttatt acttctcata ctacatttac acaagaagat tacggtgatt ctccgattcg
     6721 ttttgttgaa agaatgggtc taacgaaaga agaatggaaa aaagaacaga aaatcactct
     6781 gctacgtgcg gctatcatga ctccttattt gaatgatgat cgaattttca atttttatac
     6841 aaaaaaaatc gccaaagcaa tggaaaagaa actaaacgaa atcatccaat agaactgatt
     6901 tttgtgtgaa agaataacaa gagacatatg gagaacagac aattatttgc ttcatcaaaa
     6961 caattgtatt tccccctgtt tttcatatat tcctcgccaa gatattcttt tttataaaac
     7021 gttctataag ttggatcgtt cgtttcatgt atcaaaccca gttcactaag atcgttcaat
     7081 ggatgtttcg gtgatactgg gttttgtact gaatcgatga aaccttgtca tttctgttct
     7141 tgaacaaaaa taaaagaaaa caaaaacata tgtcgctatt gaaagaagga ataaagatga
     7201 aagatactgg ttcatcaaag caaatcacga ccatgacctt tatcggaatg acctgcgccc
     7261 ttgtagcaag tgtacgaaat attccagatg tagcagcaac gagctggacg atgatttttt
     7321 acatgctggt tgcagtatta ttgtacgctt ttccaatctc attgatctct ggtgaatttg
     7381 ctggaatgtt tccccaaaaa ggtggtccag aactttgggt atcgaatgct ctaggaaaaa
     7441 aatgggggtt cgttgtctca tggctgttat gggtccaaat gtttcctggg atggtgatgg
     7501 tcgcttcagc actcgctccg ttgtttggta atattatcga taatgtgccg cttggtttga
     7561 acagcaaatt tacattagtt gtcattttag tcgtttattg gatcatcact tttttgaatt
     7621 tgaaatttga tatggcaaaa atcggcggaa aagtcggtgt ttggttagga ttgtacgtgc
     7681 ctttgacgat gatgctctta ttagggtttg ctgcgtggat caaagttggg atcgtaccaa
     7741 ccggaacatt aggcagtttt tcatgggaca aattaatacc agatactacg actgcatcct
     7801 catttgttta ttttgcgccg atcatgttta tttttactgg tatcgaaatg tcctctgtgt
     7861 atatcactcg attggaaaat ccagttaaaa cgtatattcg cggagtattt gccgcgttaa
     7921 tttttatatt tttcgtcaat attttgaatg ctttagttgt agcaaatgtc gtaccaaaag
     7981 gacaaatgga attgaataat atcgcgcaat ctatttcgat ttattgccaa attcttggtt
     8041 tgccacatct tattgtgaat ctgtttagtt tacttgtctt cattggtgta gcagtccaat
     8101 tatctgcttg ggcctcagga ccagcaaaga cagtgacaga aagtgctaga aaaggtgcgt
     8161 atccaccaaa attcaatttt tggaagacga atcaatttga cgtatccaaa tccgttattt
     8221 tgactcagtc tgtcattatc tcaatctttg cactttttta tttgctgatt cctggcgtca
     8281 atcaagcatt tttgatgctc gtaaattcaa ctactgttat ctactgtatc gtctatgtca
     8341 tcatggggat cgcagttttg agattgcgtt atacacatgc aaagttgaat cgaccattta
     8401 gaattggaaa aaaagagaaa aagagcaatc ttggagtttg gactgtcgtt attgttttat
     8461 ttgcggcaat cggattttct gtcggactga cgatgaaagc tggaacatgg atcaatctaa
     8521 ttgcagtcac agccatcagt gtcattttgt ttgtagtacc actctgtatt gaaaaaataa
     8581 agaaaccttc ttgggaacag gaagtcgcca tgctgcttga aaaagaaaaa ggtgaacttg
     8641 atggaaaaac cggcaatgaa acggactcta actaatcgaa atatccagtt aatcgcatta
     8701 ggtggcgcaa ttggaacagg attattttta ggttcagcaa aggcaattgc atcagcaggg
     8761 ccagctgtta ttctcgttta tttgatatgc ggtttggcta tttatggttt gatgcgggcc
     8821 atgggtgatt tatttttagc aaatagtaca tttaactcta tcagtgattt cgttgactat
     8881 tatttaggca aaaagtgggc attttttgtc aattggactt attggctttg ctggcttgga
     8941 gctggacttg cggaattaac agcaatagga ttgtatatcc atttctggtt tgtacatgta
     9001 ccagctttga taagcggagg tagcgcacta atgatcttaa tggcagtcaa ccttgttgca
     9061 gtcaagtggt ttggtgaatt ggaatctttt ttctcaatca tcaagatctt ggggattttc
     9121 ctttttatct tattgggtat aggattgagt gtctcagatt ttcataccca gtctatcgga
     9181 atcaaagaaa attttggaca gatcgatgaa ttactaccgt ttggctttgc tggaatggta
     9241 gtctcatttc ctatggtttt attcagtttt gctggaattg aaatgatcgg cttaaccgtt
     9301 ggcgagacaa gtgatccgca aaagaatttg aaaaaagcaa tcaactgtct gccttggcgt
     9361 attctctttt tctatatagg aacgattttg tcgattttat ttgttgttcc atggcattgt
     9421 ttagatttaa aagaaagtcc tttagtgact atgctgcaag tgatccattg ccaaggcgga
     9481 gcagcaattg tcaatctaat gattttggtt gcttctttgt cctcagcaaa tagtgcagtc
     9541 tacagtacta gcaggatttt atatcaaaca gcaaaagaaa aaagtttgcc ccattggttt
     9601 tgtgcgcttt ctaagaaagg cgtaccttta cacggtattt taattacagg aatgttattt
     9661 tttgccggtc tttgtctaca tttttttgta gctgatagtc gattgttttt tcagcttttg
     9721 gcaggcatga caacgagttg ttttctattt gtgtggtctt cgatcttgtg tgcacatgtt
     9781 caatttatta aagaaaagaa acggaacact acacaaagat ataaagatct tgctttgttg
     9841 gtgtttttta cttcaattgc tttcagcttg ttattcgaaa gcacgacacg ctttattcct
     9901 tttttattgg ttatctggtt tgtattctta aattttgtat ataaaagaat aagcaataga
     9961 aatatcagtt aggcccagat acattttgca aaacgaatta tttcagaccc ccctgaaata
    10021 atcgatacgc ctgaaaaaac ttccctgcgt ataataacag tatcaattaa acaggagggg
    10081 ataggatgaa ctggtacaga ttgacggtag aagatgtatt gaaaaactat caaacatcaa
    10141 cagaaggctt gtcgcaagaa gaacgacaga aacgcctgac tgatcaagga tataatgaaa
    10201 taaaagcaag gcagcagaca aaacgatgga aaaaaatagc aaagcatttt actgatctgc
    10261 tgatgatcgt gttgatcatt gcctcttttt tgaaatttgc ttctagtgaa tatattgaag
    10321 gaagtattat tttattcgta gtgatcgtca atggactcgt tagttactgg caagaaagaa
    10381 aagcggaaga atcattgaat ggtttgaagc agctgatggg acaagaagcg atagtactat
    10441 caaacgggat gcaagaaaaa atccctgcga aagaactggt aaaaggagat gtcattgtct
    10501 tacgtgccgg agatgtcgtt ccagcagata tccgattaat cgaagcacat gatttattag
    10561 tggaagaatc gattttgacg ggagaatcag aagcatctga aaaaagtcat gccgttttga
    10621 cagaagaaga atcgatcggc gaccagaaaa atatgggttt ttcaggaacg cttgtacaat
    10681 ctggttctgc agtcggtgtc gttgtagaaa caggaaacac gacagaaatc ggtaaaatca
    10741 atcaagcatt gcaatcggtc gaacaacaaa cgacgcctct gattaaaaaa atcaaccgat
    10801 taaacaaaca aatttttcaa ggcattcttt gtttgagcct attcttagtt atttttacga
    10861 catttcgata tggtatggat tggcatattc ttctttcggc tacgattgct ttggtggtgt
    10921 caatggtacc cgaaggattg ccagctgttt tgaccatgat tctatccgtt ggagtacatg
    10981 aaatggcaaa agaaaaggcg atcatcaaag gacttccttc ggtagaaaca ttaggttcta
    11041 tgacagtgat ctgttcagat aagacaggaa cattgacaaa aaatgaaatg acagttgtgg
    11101 atgtagctgc aaaagaagaa gcctgcgtgt tgtccattat gaaaaactgt caggaattaa
    11161 aaacaaaaga agaacagaaa acagaaaatt taagtggcaa tccgacagaa gttgcgctga
    11221 ttcgacacac agaaacagcc aatctacctt tgaaaaaaat agaagcaaag attccgttca
    11281 gttctgaata caaatatatg gcaacgatgc atgcggagga aaaaggagcg gtgatttacg
    11341 tcaaaggtgc accagaagta ttatttgcta aatcaactct ttcttctgca gaacaagaag
    11401 catggagtca gacagctgca gaatttgcga gaaaaggtca gcgtgtttta ggatttgctt
    11461 ataaaaaagt agattcgaaa caggaactca cgcatgagac tttaacccaa ttaacttttg
    11521 cagggatcgc cggattgatc gatccgccaa aagaaagtgc agtcaaagct gtaaaggaat
    11581 gccaacaagc agggatctca gtgaaaatga ttacagggga tcacaaagac acagcaaaag
    11641 cgattgcgga acaaattggc ttaaaacata ctgccaaagt tttagaaggg atcgatttgg
    11701 atcttatgtc tgatgaagag ttgattcagc aagtgccaat agtcgatgtc tttgctcgaa
    11761 cgacaccaga acataaactg cgaattgtta aagctttgca gaaaaatgga gaaatcgtcg
    11821 gaatgaccgg agacggcgta aatgatgcac ctgctttgaa aagatccgat gtaggaattg
    11881 ctatgggaat caaaggtagt gaagtaagca aacaggcagc tgatatggtt ttaggagatg
    11941 ataatttcca tacgattgca aaagccgtaa aagagggccg gcggattttg gataatttgc
    12001 aaaaaaccat caatttcttc ttgccaactg cattagctca aggattgatc ttgatttggg
    12061 cacttatgct gaatcgcccg ctacctttgt ccccagtaca gattttatgg gtcaatatgg
    12121 taacgacaat cactttatct tatgcactag ggttcgagtc gccagatcca gaaatcatga
    12181 agcgcccacc aagagatcca aagcaaggaa tcctatctgg ttatcatttg tttcggatca
    12241 tctatgtctc cctactgatc atggttcctg cctatttatt ggctatgcag tttgaagggc
    12301 aaagcttaca gcaaaccatt cttctgcaaa atatcgtctt agctcaagct gtttacatga
    12361 tcaattgcag agagttgtcc aagccgtcat tgaaccaagg tcttttgcaa aacaaagcgc
    12421 tttttctttc attaggtatc cttgctttat tgcaagggat ggttattttt ctaccagtcg
    12481 gccagcagct gatcggtaca gtaagtttaa cgatgacaca acagttattg attggagcaa
    12541 atgtgattct tctattctta gttgtagaaa tagaaaaagg gatcatgaac aaactgtttc
    12601 gcaaaaaaga aaaaacagct agagaataca gtcgctagcc gttactataa aaaagttttg
    12661 aaagtgagtt cagtcaaacg attgagctca tttttcgttg gaatagaaga aatgtataca
    12721 atagggatat ctagagaatc tagagggaaa ttggaaatca agatatctgt tttttcaaaa
    12781 gaaatagtgg aaagatcggt tagttcgata ggtaagaagc agacacgttt tcctaagtaa
    12841 tcttttaatt cctgctgcaa gaacgccttc catgaaggtt ctccttggaa aataaaatag
    12901 acattgaaca aattcggttt gatagaaaaa atcgtttgtt ggatcagtag agacaagagt
    12961 tggagaaagt aatttggttc attgatttct atttcttttt ttagatcgtt cagtaaattc
    13021 tctttctgtt gatacaagtg tgcatattgc tgatcatatg tttctaaaaa acgatggtat
    13081 tgacggtcca atgcttcaat caatatcggc gattcatgat attttaatat gaggagagcc
    13141 aagttatcag tcagagatgt tttttttagt tgatcaagag acagctctga agctagttcc
    13201 tctacaaacg tttcgaggac ctctaaaaat ggagaatgat gcaggcagcg agcggtttct
    13261 tgtccatagt tattgttgta attccaactt tccagaaagc agaaagaagc aaaagaaagc
    13321 tcagtgtctt taagataaat accttccgtt aaatacaatt ttttcaatgc agagtcatac
    13381 agggaggaga gtgtgctgtt ggtatggata gaagaatcta atcggcaatt cgcttttata
    13441 cggatcgtat tgataaagaa ccaacatgtt ccgaaaatct cttctgtttc aaccgctagt
    13501 tcagtttgac tgaggtttct taaaaattga aaataatggg attcatggat cgaataatcc
    13561 tcgaaaaaat agttgttatg agtgaaagga atcagcaatc gatgataaaa agtacgaatc
    13621 gcacgctctt ttccaagaat acaagctgta tatttcgtca aatcgatctg caagtcgtaa
    13681 cttgctaaac gctgattgat tttagtcaca tgtcttttca atgtcccata agaaagccca
    13741 tggtcggcta gaaaatcagg gacagcaact ttttttgtaa aaagcagatt tttcagcagt
    13801 tcgacactga ctgattgttt gaaaaaaatc tcccaaaggt catttgttaa taaattttgc
    13861 tggttttgca aactgattcc tgcttgatcg ctgatgatgt cccagccttc gggcagctga
    13921 gagcgaatag attgaaggtc agaaaaaatc gttcgttcgg ttgtccctat gatttcagct
    13981 aattcattta cggttatctt tgtatgattc agcaattgtt ctactaaaga aacatgacgt
    14041 cgaatatctt tttcggtaat gatttttttc attagtgaat acatcaatca gactcccttc
    14101 tattctaaga atacggagaa aataaacggt ggtcaagttt caactgattt ttcaatcatt
    14161 gtttattttt ctggagcatt tactgatgat aagaatttca tcatgaactt gaaaaatgat
    14221 accactaaaa tcaaaaaata ggtgggcaaa caataataaa cattttaata gtagatggta
    14281 gcaagaccaa cacaggcgga ttgaacattt aagagttgat aaaaaagaaa aatatcgaag
    14341 tatttgatta gcaactactt gtattcattt ttctattctt acgtggtatc tttgaataaa
    14401 gcgaatcaat aagaaagaga tggaagcaga tgctaacgaa aaaagaacta atcgatcaca
    14461 tcaatgaaac atacaatttg tctccagaat atatttttaa aaaatttcct gattattgcg
    14521 tattcagaca tcaaaaaagc aaaaaatggt ttggggtcct gatgtctgtt ccagaagaaa
    14581 aaatctatga tgatgcaagt gaccatagga tcatcaatat tcttgatttg aaggtcgatc
    14641 ctgaacttgg cggaattttg cgaggaaaag aggggtattt tccagcctat cacatgaata
    14701 aagaacattg gatctctatt gatctacgta aaatagaaaa gctagatgaa ctagcagatt
    14761 tgattgatgg aagttttaaa tcaacgcaat gaatgtctag gacaatagag aagaggttgt
    14821 gacagaaatg tt
//
LOCUS       DBNIBO010000060        14596 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.064, whole genome shotgun sequence.
ACCESSION   DBNIBO010000060 DBNIBO010000000
VERSION     DBNIBO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.064"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(168..1586)
                     /gene="nhaC"
                     /locus_tag="AC1Y77_001521"
     CDS             complement(168..1586)
                     /gene="nhaC"
                     /locus_tag="AC1Y77_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIQ1575100.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1989..3212)
                     /locus_tag="AC1Y77_001522"
     CDS             complement(1989..3212)
                     /locus_tag="AC1Y77_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIQ1575101.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3243..4082)
                     /locus_tag="AC1Y77_001523"
     CDS             complement(3243..4082)
                     /locus_tag="AC1Y77_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIQ1575102.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4099..4779)
                     /locus_tag="AC1Y77_001524"
     CDS             complement(4099..4779)
                     /locus_tag="AC1Y77_001524"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIQ1575103.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4781..5854)
                     /locus_tag="AC1Y77_001525"
     CDS             complement(4781..5854)
                     /locus_tag="AC1Y77_001525"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575104.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5910..6148)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6191..6424)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6501..7172)
                     /locus_tag="AC1Y77_001526"
     CDS             complement(6501..7172)
                     /locus_tag="AC1Y77_001526"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575105.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7259..9907)
                     /gene="mgtA"
                     /locus_tag="AC1Y77_001527"
     CDS             complement(7259..9907)
                     /gene="mgtA"
                     /locus_tag="AC1Y77_001527"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HIQ1575106.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(10249..10423)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            10596..11033
                     /gene="ndk"
                     /locus_tag="AC1Y77_001528"
     CDS             10596..11033
                     /gene="ndk"
                     /locus_tag="AC1Y77_001528"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIQ1575107.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11055..11921)
                     /locus_tag="AC1Y77_001529"
     CDS             complement(11055..11921)
                     /locus_tag="AC1Y77_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIQ1575108.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12081..14174)
                     /locus_tag="AC1Y77_001530"
     CDS             complement(12081..14174)
                     /locus_tag="AC1Y77_001530"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIQ1575109.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4235 a         3116 c         2487 g         4758 t
ORIGIN      
        1 aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa atggctttcc
       61 acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac cgtttattcg
      121 taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta ttgttctaat
      181 tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat tccccaacct
      241 gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac gaatggcaag
      301 taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat tccccaaggc
      361 actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac agttggatca
      421 aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac agataggaat
      481 tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa aatcaaattt
      541 ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa gccaaattcg
      601 atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg catcatactt
      661 tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt tgaaacaaac
      721 cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg aataaaaatc
      781 attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac ctttttccat
      841 gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt aaagtttgct
      901 tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact tgtgtgtcct
      961 aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca catcatatgc
     1021 ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt tgattcggat
     1081 aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac aaccagcgct
     1141 ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt tgacataaca
     1201 gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac agaagggaca
     1261 aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat gatccctgct
     1321 tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg gatgattcct
     1381 ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct ttttatcact
     1441 gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga gacctttaat
     1501 ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt tgcttctaaa
     1561 aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt tcttatgttc
     1621 taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc tcctttataa
     1681 aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac cttaattaaa
     1741 aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc catgtaattc
     1801 gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa cacctaaccg
     1861 ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat ttgtcaatga
     1921 aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac acacaagttt
     1981 attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc ttgatgttct
     2041 tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt attggcgagt
     2101 agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc agctttgaat
     2161 tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg gattgttggt
     2221 atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg ttccgtttct
     2281 atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc atcaaaagaa
     2341 ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa agtcgtgccc
     2401 gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt atctaatgtt
     2461 tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat gacgttggca
     2521 gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa ttgcaagcgt
     2581 agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac tcctttttca
     2641 ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg atcattcgct
     2701 aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc tacatcgaca
     2761 tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc gcccccttct
     2821 tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa ttgcttcact
     2881 atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc atgaccaata
     2941 cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt attggaaata
     3001 taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca aaacctttct
     3061 ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact tacttcagga
     3121 tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg gattagatcc
     3181 ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt
     3241 tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga tgttttctcg
     3301 attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata cgttttattg
     3361 ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg gtcatccact
     3421 ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc tacaccgcta
     3481 ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc atctaattca
     3541 gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc ttgtcctttt
     3601 gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct gccgccattt
     3661 gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac atccttcaaa
     3721 gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac taaatcagtt
     3781 ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc
     3841 gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac gtattccaaa
     3901 ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt atcttctcca
     3961 ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt tccttggcta
     4021 caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat aatttttttc
     4081 attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac tgataaattg
     4141 acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat ctgtttggaa
     4201 tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta ctgctccagc
     4261 catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa tgataccagg
     4321 caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc ccatcgcttc
     4381 tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa
     4441 aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc
     4501 aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag ggatggaacg
     4561 aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta gtaatccatt
     4621 agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc caatgaatgc
     4681 tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa
     4741 ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct ccttcgatca
     4801 ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca ccagatttga
     4861 tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta acgatcaaac
     4921 tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc gctgttactt
     4981 gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca atataactta
     5041 attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca atgatcgttt
     5101 ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat tcttcttcag
     5161 gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct tccataaccg
     5221 ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg acgattgtta
     5281 aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc gttttcggat
     5341 caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc aaagctcgtg
     5401 cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca gatgcttttt
     5461 cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc tgtttcgatt
     5521 ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt tccattagat
     5581 tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct ttgggagaga
     5641 gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc tgcagcaaat
     5701 tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt ccataaacat
     5761 cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt tctttttgtt
     5821 tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag
     5881 cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt gttaccacct
     5941 catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact ctagcaagat
     6001 atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc gatggccatt
     6061 ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct
     6121 atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt actaacataa
     6181 actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag tttccttccc
     6241 tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga acagttccga
     6301 atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact
     6361 cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt ttcaacgaaa
     6421 tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca agtacgaaat
     6481 tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc aataacagtt
     6541 cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac ggtataattt
     6601 cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga agcagctgtt
     6661 gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc tgttcgttcc
     6721 aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc tccgaatgta
     6781 aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc tcttttcgta
     6841 acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc caatatttct
     6901 tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa atattcttca
     6961 cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac taaaatatca
     7021 aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg gatatggata
     7081 tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt gtcaactaac
     7141 aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata
     7201 cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc cttctcgttt
     7261 ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca agcgccagat
     7321 aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc atcaagccta
     7381 attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct aatgtagtga
     7441 taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt gtacgcagtg
     7501 catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca tggaataatg
     7561 cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc actacagcag
     7621 gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa cttgttggac
     7681 caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc tcgtccaaat
     7741 attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag atcagattca
     7801 aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt gcaatcagca
     7861 cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc atgatattcc
     7921 caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct ttttctaaaa
     7981 ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa ataccgatat
     8041 cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg acagtatgac
     8101 catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat tttacgaata
     8161 cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag attttctcac
     8221 cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt gtaactaatt
     8281 cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct ttcaacgctt
     8341 gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa accatgtctg
     8401 actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt gcgacaccaa
     8461 tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct tcttttcgtt
     8521 cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg ttcagcattt
     8581 cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca accacaacac
     8641 tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa ttgatcttat
     8701 ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa tttttcagcc
     8761 ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct tcatttcctt
     8821 cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc gttttatccg
     8881 tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg atcactgttc
     8941 cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc attggaagca
     9001 tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct agccaatccc
     9061 cttttgtaag cccattgatg acgataactg ctggagccat caatgacata aatttgatca
     9121 gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt ttttcagata
     9181 gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg attccttccg
     9241 cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt gtttcgcttt
     9301 gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc cctgtcaatg
     9361 ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct ggtatcatat
     9421 cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga atctctgtgt
     9481 actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt ttttcagccg
     9541 cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata accatagtga
     9601 agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca tattccgtga
     9661 tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg tatgctttca
     9721 atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca ccgtaatttt
     9781 ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg aatagttcaa
     9841 ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta tctttctttt
     9901 tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc ttttggtctt
     9961 tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag atagataata
    10021 gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt tgttctcatc
    10081 tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag ttatttttcc
    10141 aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca aaaaaacctc
    10201 tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc tcagatctca
    10261 tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag ctaccttatg
    10321 aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg tttttgggca
    10381 gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt ccttcgttaa
    10441 tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata ctttttttaa
    10501 aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc cacctcccgc
    10561 tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt gttattatca
    10621 agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt gaagcaaagg
    10681 gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca aaagaacatt
    10741 atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg acttccggtc
    10801 ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt aagatggttg
    10861 gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac gcgctgccgg
    10921 ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa gagatcgccc
    10981 gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag tgatacaaat
    11041 tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt ctctaaggac
    11101 ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg gtagtctttc
    11161 gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc catcaatgtt
    11221 tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc ttcattcaca
    11281 ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt cattaaaaaa
    11341 gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct ttttttgatt
    11401 ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc agctagaata
    11461 gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat gattcggcat
    11521 tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac catgtgaaga
    11581 ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt attgctgatc
    11641 tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct ttttttcttt
    11701 aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag tgtcatgttc
    11761 attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga atttgtctca
    11821 atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg tgtcaacttc
    11881 ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct cctttttaat
    11941 ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca cgaaaaaaat
    12001 aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt tgtctaacta
    12061 ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc tgttcttgtt
    12121 actggatata agttggttgt tgttttatta ccattttttg aaaagacact tgtcgaatgt
    12181 tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata gtcatatttt
    12241 ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt ttcctgattc
    12301 agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga gatttcctca
    12361 tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta agcttttcct
    12421 cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac tacttgatca
    12481 tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc gattcccaat
    12541 agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt gactgtccct
    12601 ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc gttgtcaaat
    12661 tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt atctgtattc
    12721 gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat accagatttt
    12781 ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt tcgaacataa
    12841 ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt gtcatccgta
    12901 taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg ttgtagatgc
    12961 tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata acctaacgtt
    13021 tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa gtttttatat
    13081 acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct tgtatagcct
    13141 tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc aagttctgtg
    13201 aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc agcatcactt
    13261 gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc ttgattctgg
    13321 aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt aatcaaaaac
    13381 tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat cccaaatgtt
    13441 tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc aatcccatta
    13501 ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc ataaatagta
    13561 tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg ctgcagcaat
    13621 tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc tgaaagacag
    13681 gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat tttcttgacc
    13741 ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat gacatcatgg
    13801 ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga aaagcctaat
    13861 acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt caagtacaac
    13921 aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt tggcttttta
    13981 atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat ccacaagata
    14041 aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc aatgatcgtc
    14101 ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg gcacaatttt
    14161 ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt ttgctcctga
    14221 aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt tctaaagatt
    14281 attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa ctaatgaaga
    14341 gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt ttctcggtat
    14401 tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt cttgatgcag
    14461 gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc tcggccacaa
    14521 gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg tggctcagag
    14581 ttaaacggca ggctca
//
LOCUS       DBNIBO010000061        14119 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.065, whole genome shotgun sequence.
ACCESSION   DBNIBO010000061 DBNIBO010000000
VERSION     DBNIBO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.065"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..556)
                     /locus_tag="AC1Y77_001531"
     CDS             complement(<1..556)
                     /locus_tag="AC1Y77_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIQ1575110.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="AC1Y77_001532"
     CDS             complement(572..1051)
                     /locus_tag="AC1Y77_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIQ1575111.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="AC1Y77_001533"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="AC1Y77_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="AC1Y77_001534"
     CDS             complement(2098..3204)
                     /locus_tag="AC1Y77_001534"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HIQ1575112.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="AC1Y77_001535"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="AC1Y77_001535"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIQ1575113.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="AC1Y77_001536"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="AC1Y77_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIQ1575114.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="AC1Y77_001537"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="AC1Y77_001537"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIQ1575115.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="AC1Y77_001538"
     CDS             8175..8873
                     /locus_tag="AC1Y77_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIQ1575116.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="AC1Y77_001539"
     CDS             complement(9041..11680)
                     /locus_tag="AC1Y77_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1575117.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="AC1Y77_001540"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="AC1Y77_001540"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIQ1575118.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14119
                     /locus_tag="AC1Y77_001541"
     CDS             13523..>14119
                     /locus_tag="AC1Y77_001541"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575119.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2544 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagcg
//
LOCUS       DBNIBO010000062        14103 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.066, whole genome shotgun sequence.
ACCESSION   DBNIBO010000062 DBNIBO010000000
VERSION     DBNIBO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.066"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            65..766
                     /locus_tag="AC1Y77_001542"
     CDS             65..766
                     /locus_tag="AC1Y77_001542"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIQ1575120.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="AC1Y77_001543"
     CDS             763..1884
                     /locus_tag="AC1Y77_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIQ1575121.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="AC1Y77_001544"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="AC1Y77_001544"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIQ1575122.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="AC1Y77_001545"
     CDS             3263..4171
                     /locus_tag="AC1Y77_001545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIQ1575123.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="AC1Y77_001546"
     CDS             4206..4478
                     /locus_tag="AC1Y77_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIQ1575124.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="AC1Y77_001547"
     CDS             4489..5535
                     /locus_tag="AC1Y77_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIQ1575125.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5786..8395
                     /gene="clpB"
                     /locus_tag="AC1Y77_001548"
     CDS             5786..8395
                     /gene="clpB"
                     /locus_tag="AC1Y77_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIQ1575126.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8546..9232)
                     /locus_tag="AC1Y77_001549"
     CDS             complement(8546..9232)
                     /locus_tag="AC1Y77_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575127.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9210..9704)
                     /locus_tag="AC1Y77_001550"
     CDS             complement(9210..9704)
                     /locus_tag="AC1Y77_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIQ1575128.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9724..10944)
                     /locus_tag="AC1Y77_001551"
     CDS             complement(9724..10944)
                     /locus_tag="AC1Y77_001551"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575129.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11091..12926
                     /gene="lepA"
                     /locus_tag="AC1Y77_001552"
     CDS             11091..12926
                     /gene="lepA"
                     /locus_tag="AC1Y77_001552"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIQ1575130.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13020..14102)
                     /locus_tag="AC1Y77_001553"
     CDS             complement(13020..14102)
                     /locus_tag="AC1Y77_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1575131.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4554 a         2591 c         2920 g         4038 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaacgt
     5581 tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac aatcaatttt
     5641 gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg tatattttga
     5701 ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata agtgatctta
     5761 cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga cgcttcaaga
     5821 agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata tcgacattgc
     5881 ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct atacggatgc
     5941 cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg agtatccagt
     6001 tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct ttcgattatt
     6061 gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt
     6121 tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga ataaaaacgg
     6181 attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag aaagagtgac
     6241 ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg atttagtaca
     6301 gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa tccgtgatgt
     6361 catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag aacccggtgt
     6421 agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga
     6481 aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg ctggagcaaa
     6541 attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca aaaaaagtga
     6601 tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga
     6661 aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg aattacattt
     6721 gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca aagcgttgga
     6781 acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga tcagtattct
     6841 gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg acaacgcatt
     6901 agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac cggacaaagc
     6961 aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact ctatgccaac
     7021 ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag ctgcactgaa
     7081 aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga
     7141 acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa
     7201 ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag aagatgcaga
     7261 aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac ctcaattaga
     7321 aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga tggttcaaga
     7381 gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta tccccgtaac
     7441 gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac ttcataaacg
     7501 agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc gttcacgggc
     7561 tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac caactggtgt
     7621 cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag aagatcatat
     7681 ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc tagtcggagc
     7741 tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg tcagacgaaa
     7801 tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg tcttcaatat
     7861 cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag tagtagattt
     7921 caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc tggaaggcgt
     7981 gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg
     8041 aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta caccattgag
     8101 cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac aacgtctgga
     8161 acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg ctgaaaatgc
     8221 ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg aagtagaaac
     8281 accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag taacgattac
     8341 tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg tctaatcttc
     8401 cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc tccttttttc
     8461 aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt cttgaagctg
     8521 cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat ttataacttt
     8581 caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt aaggcagcac
     8641 ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca
     8701 gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg tcgcataaga
     8761 attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg aataagagga
     8821 acaaccaagg actggttttt gatatctcag catctttcat caagtaacca atcaatccat
     8881 agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc caaaaagaga
     8941 tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag aaaaaccagt
     9001 aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg acttgtggca
     9061 tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct tgagaagtcg
     9121 aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca ccaaaagcaa
     9181 gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc atctaaaaat
     9241 ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt caatttgatc
     9301 aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc ttgtagtgca
     9361 gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa gccgatcact
     9421 gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc gatcgtcagc
     9481 ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc agcaagagca
     9541 atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc ttgaggaaga
     9601 tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg gttatataac
     9661 aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac atcatccttt
     9721 ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct ctagtgactt
     9781 cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag cacggcgata
     9841 attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc ctataggttc
     9901 atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac cattattcca
     9961 aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa caaacggaaa
    10021 ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt cgatctgctc
    10081 tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa tatctccatg
    10141 tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat ttcgagcttc
    10201 ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc agtagaacag
    10261 aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct catcttcatt
    10321 ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac catggttttg
    10381 ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta atcgctcaac
    10441 cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa aggaaagttt
    10501 atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag gatacaaata
    10561 agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta gatcttgcat
    10621 caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac ccattttata
    10681 acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga tcagtttatt
    10741 ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg aacggataaa
    10801 agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt ggaacacata
    10861 ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta attcaagatt
    10921 ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga taacagtata
    10981 gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt aaattactgc
    11041 ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct atggatataa
    11101 agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct catatcgacc
    11161 atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg acgtctcgcg
    11221 aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga atcacgatta
    11281 aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat atctttcact
    11341 tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc cttgctgcct
    11401 gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa acattggcca
    11461 atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat aaaatcgacc
    11521 tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg
    11581 ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat attctcgagc
    11641 agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta aaagctttga
    11701 tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg atcacggacg
    11761 gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta
    11821 cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg
    11881 tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg gatacagtaa
    11941 cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg aatccgatgg
    12001 tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg gaagctttag
    12061 aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct caagcattag
    12121 gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc caagaacgct
    12181 tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt taccatgtta
    12241 ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct gaacctgtaa
    12301 cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca aatgattttg
    12361 tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg atggattatt
    12421 tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt gtctttgact
    12481 ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat gaaatgtctg
    12541 gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa aaagtcgatg
    12601 cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca atcgttgaaa
    12661 aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca gctattggac
    12721 aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt
    12781 atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc
    12841 ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa
    12901 tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt aaaaaataaa
    12961 ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg tggcaaaaat
    13021 caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat cttatctgtt
    13081 acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg ttcagagatt
    13141 tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct gagagaatgg
    13201 aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt cactacactt
    13261 ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat gtagagcttt
    13321 ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat tgctagcaag
    13381 gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc atatacccat
    13441 gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga aaaatcgaca
    13501 tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa tccaatattc
    13561 aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc aaagtcctca
    13621 agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt tggtattttt
    13681 acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat attcagcgca
    13741 ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt tttaaattca
    13801 gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt ctttcctaaa
    13861 taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt gtattttaga
    13921 tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg agtatccgta
    13981 cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa agcagcttcg
    14041 gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc atcatcattg
    14101 ata
//
LOCUS       DBNIBO010000063        14075 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.067, whole genome shotgun sequence.
ACCESSION   DBNIBO010000063 DBNIBO010000000
VERSION     DBNIBO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.067"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(105..1562)
                     /locus_tag="AC1Y77_001554"
     CDS             complement(105..1562)
                     /locus_tag="AC1Y77_001554"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1575132.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1559..3454)
                     /locus_tag="AC1Y77_001555"
     CDS             complement(1559..3454)
                     /locus_tag="AC1Y77_001555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1575133.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3512..5287)
                     /locus_tag="AC1Y77_001556"
     CDS             complement(3512..5287)
                     /locus_tag="AC1Y77_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIQ1575134.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5337..6833)
                     /locus_tag="AC1Y77_001557"
     CDS             complement(5337..6833)
                     /locus_tag="AC1Y77_001557"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1575135.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7027..8649)
                     /locus_tag="AC1Y77_001558"
     CDS             complement(7027..8649)
                     /locus_tag="AC1Y77_001558"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIQ1575136.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(8977..10290)
                     /locus_tag="AC1Y77_001559"
     CDS             complement(8977..10290)
                     /locus_tag="AC1Y77_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIQ1575137.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10535..10759)
                     /locus_tag="AC1Y77_001560"
     CDS             complement(10535..10759)
                     /locus_tag="AC1Y77_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575138.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10779..11201)
                     /locus_tag="AC1Y77_001561"
     CDS             complement(10779..11201)
                     /locus_tag="AC1Y77_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIQ1575139.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(11343..12053)
                     /locus_tag="AC1Y77_001562"
     CDS             complement(11343..12053)
                     /locus_tag="AC1Y77_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B3/B4 domain-containing protein"
                     /protein_id="HIQ1575140.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12071..12568)
                     /locus_tag="AC1Y77_001563"
     CDS             complement(12071..12568)
                     /locus_tag="AC1Y77_001563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575141.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12605..13057)
                     /locus_tag="AC1Y77_001564"
     CDS             complement(12605..13057)
                     /locus_tag="AC1Y77_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1575142.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13192..13797)
                     /locus_tag="AC1Y77_001565"
     CDS             complement(13192..13797)
                     /locus_tag="AC1Y77_001565"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIQ1575143.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         4139 a         2939 c         2265 g         4732 t
ORIGIN      
        1 atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa
       61 ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag gatttcgcca
      121 tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg tttctttgtg
      181 ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga catttgtgaa
      241 gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa atctactcca
      301 tctttgatag cttccttcat ctgctcaata tgctgcttca aatagtcaat gcggtaatcg
      361 tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt ttcagctatg
      421 aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa acccttagga
      481 tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc tccagttatt
      541 aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact agacatgtag
      601 tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc acctacttct
      661 atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg ataatatcct
      721 cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt tttttgaata
      781 tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca accgacttgt
      841 gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat aaactgatgg
      901 tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat tactcctacg
      961 ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca atattttact
     1021 ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg aataagcttt
     1081 cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata atgggaaaga
     1141 gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc ataaaaaaat
     1201 aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt ccatgcgata
     1261 gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc tttaaagtga
     1321 tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg atcctgcgcc
     1381 gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc tttaggtaaa
     1441 tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac atcccatgca
     1501 ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg aaattttttc
     1561 atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta tgactagtaa
     1621 tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga tcatatccct
     1681 ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt actttttttc
     1741 catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca ataccgatat
     1801 ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc gttggaaaaa
     1861 ctgttgaaac ttccccgttt acagaactgt agatttttcc gtccgttgga atgatcgcaa
     1921 acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat aaatcaatta
     1981 tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct tcgatttcat
     2041 ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag aaataactag
     2101 caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata cttcttgtag
     2161 caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata gctccacata
     2221 tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca gcaggttcag
     2281 tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact ttcttatttt
     2341 tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca gtcatacatg
     2401 ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact ggtacaagtc
     2461 cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca gcaccagata
     2521 aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga cttgcataat
     2581 ctgcaattgg acctacaact aataaaataa ttgggaccat cacgatgata caaatgaatg
     2641 gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc tcaatataag
     2701 aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt gctagaatca
     2761 cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca atcgtaggat
     2821 aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac ttttttgcag
     2881 cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa
     2941 tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag gcgagtactc
     3001 ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca
     3061 tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca ctagatgcat
     3121 catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact gcatttccta
     3181 ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct agttgctcta
     3241 ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt gttgcacaat
     3301 ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt
     3361 gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc cttcttacaa
     3421 gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt ctaatagcga
     3481 acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc actgcttgaa
     3541 agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca cgcttgatct
     3601 tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt gtttctactt
     3661 ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata actaggtcag
     3721 aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt atttgatttt
     3781 cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact atggtcagct
     3841 tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca ctaggtagat
     3901 aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt gcatcatatt
     3961 ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg cattctttga
     4021 tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata ctaattggct
     4081 ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct tcggcaagat
     4141 acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga
     4201 aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt acaaaatgat
     4261 tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg gttagcaaaa
     4321 attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata atcgtttgct
     4381 caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc atcaaataat
     4441 ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa gaatagggta
     4501 gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat gtatacctgt
     4561 ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc
     4621 gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct tttaatgtct
     4681 tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgagga aaccttgcgt
     4741 gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa ttttcttcag
     4801 caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga ttgcgctcga
     4861 tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga aagaaaatgt
     4921 ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc acaatagttg
     4981 aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt atgatttgct
     5041 gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt gactggataa
     5101 tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc gttttcttgt
     5161 tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta tttcttaata
     5221 ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc ctctttgtta
     5281 taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt taaaaatcat
     5341 ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg tttataatat
     5401 tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca
     5461 taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt
     5521 ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga
     5581 agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt
     5641 gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc
     5701 aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga
     5761 gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt
     5821 tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg
     5881 ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc
     5941 catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat
     6001 gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa
     6061 gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc gattttcttt
     6121 aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact
     6181 ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca
     6241 gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc
     6301 actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat
     6361 tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc ataatgacta
     6421 agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat
     6481 acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag
     6541 atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat
     6601 cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc
     6661 tcccaaatct ttttttcttc attccactga atactaggat gctggttcat gatcccgata
     6721 agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta
     6781 gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc
     6841 tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat
     6901 tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt
     6961 tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca
     7021 aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc
     7081 tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaatagt
     7141 gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat
     7201 gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac
     7261 gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc
     7321 ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca
     7381 tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc
     7441 attgtctctt cctttcgcct tgattgattc caagatttcc tcttttgtaa atccagaaga
     7501 aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat
     7561 tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat
     7621 atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca
     7681 acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc
     7741 acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt
     7801 caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac
     7861 catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc
     7921 ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg
     7981 aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat
     8041 catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat
     8101 ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct
     8161 atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa
     8221 tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc
     8281 ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt
     8341 atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag
     8401 ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc
     8461 atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc
     8521 taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg
     8581 atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt
     8641 ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt
     8701 tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta
     8761 acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga
     8821 tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt
     8881 cccttggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc
     8941 taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat
     9001 gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt
     9061 agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact
     9121 ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc
     9181 ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc
     9241 agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa
     9301 aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga
     9361 ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga
     9421 atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg
     9481 gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag
     9541 cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa
     9601 tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt
     9661 gaccagacct aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg
     9721 aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt
     9781 gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag
     9841 accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg
     9901 gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga
     9961 agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga
    10021 ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa
    10081 tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc
    10141 ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga
    10201 gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat
    10261 ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa
    10321 ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa
    10381 caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt
    10441 aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg
    10501 tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat
    10561 ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc
    10621 cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga
    10681 gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta
    10741 atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt
    10801 cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta
    10861 tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa
    10921 aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt
    10981 gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct
    11041 ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc
    11101 atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc
    11161 tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa
    11221 caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct
    11281 ccacaaactt tttttacaaa aaaaaaacgg caatgaatgc cgctcttctt atttcgattc
    11341 ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca
    11401 aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt
    11461 tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt
    11521 ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg
    11581 atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca
    11641 ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa
    11701 ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa
    11761 aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt
    11821 ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat
    11881 gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac
    11941 gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg
    12001 gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt catctgttca
    12061 cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca
    12121 tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa
    12181 catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt
    12241 caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat
    12301 agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat
    12361 ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt
    12421 tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag taatcagcta
    12481 aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt
    12541 gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt
    12601 cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct
    12661 tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta
    12721 atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt
    12781 ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca
    12841 tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca
    12901 tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat
    12961 actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa tctagcgctc
    13021 gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt
    13081 gtaagactag ttcacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt
    13141 tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca
    13201 atagatctgg tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga
    13261 tagctttggc aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat
    13321 aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag
    13381 ctgtggaata tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac
    13441 tgttaatcct gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat
    13501 ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac
    13561 cttgccatct tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc
    13621 aatcgttgtg agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc
    13681 caatgaaatc gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata
    13741 tagaccagct tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct
    13801 cttcccttct tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctggtttaat
    13861 tacggcaaat aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa
    13921 caaaagacat tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc
    13981 ggtaataagc cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt
    14041 aattgaggca ggacagcttt ttcacaacct cccca
//
LOCUS       DBNIBO010000064        13885 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.068, whole genome shotgun sequence.
ACCESSION   DBNIBO010000064 DBNIBO010000000
VERSION     DBNIBO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.068"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(175..1461)
                     /gene="yfmH"
                     /locus_tag="AC1Y77_001566"
     CDS             complement(175..1461)
                     /gene="yfmH"
                     /locus_tag="AC1Y77_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIQ1575144.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /gene="yfmF"
                     /locus_tag="AC1Y77_001567"
     CDS             complement(1454..2722)
                     /gene="yfmF"
                     /locus_tag="AC1Y77_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HIQ1575145.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="AC1Y77_001568"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="AC1Y77_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIQ1575146.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="AC1Y77_001569"
     CDS             complement(3494..3940)
                     /locus_tag="AC1Y77_001569"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575147.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4033..4278)
                     /locus_tag="AC1Y77_001570"
     CDS             complement(4033..4278)
                     /locus_tag="AC1Y77_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HIQ1575148.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4393..4971)
                     /locus_tag="AC1Y77_001571"
     CDS             complement(4393..4971)
                     /locus_tag="AC1Y77_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIQ1575149.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5048..5578)
                     /locus_tag="AC1Y77_001572"
     CDS             complement(5048..5578)
                     /locus_tag="AC1Y77_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIQ1575150.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="AC1Y77_001573"
     CDS             complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="AC1Y77_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIQ1575151.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6435..6830)
                     /locus_tag="AC1Y77_001574"
     CDS             complement(6435..6830)
                     /locus_tag="AC1Y77_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIQ1575152.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6827..8239)
                     /gene="cysS"
                     /locus_tag="AC1Y77_001575"
     CDS             complement(6827..8239)
                     /gene="cysS"
                     /locus_tag="AC1Y77_001575"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIQ1575153.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8236..8772)
                     /gene="epsC"
                     /locus_tag="AC1Y77_001576"
     CDS             complement(8236..8772)
                     /gene="epsC"
                     /locus_tag="AC1Y77_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIQ1575154.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8858..9097)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9094..10554)
                     /gene="gltX"
                     /locus_tag="AC1Y77_001577"
     CDS             complement(9094..10554)
                     /gene="gltX"
                     /locus_tag="AC1Y77_001577"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIQ1575155.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10724..11881)
                     /locus_tag="AC1Y77_001578"
     CDS             complement(10724..11881)
                     /locus_tag="AC1Y77_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIQ1575156.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11915..13285)
                     /gene="radA"
                     /locus_tag="AC1Y77_001579"
     CDS             complement(11915..13285)
                     /gene="radA"
                     /locus_tag="AC1Y77_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIQ1575157.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13348..13875)
                     /locus_tag="AC1Y77_001580"
     CDS             complement(13348..13875)
                     /locus_tag="AC1Y77_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIQ1575158.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         3644 a         3030 c         2490 g         4721 t
ORIGIN      
        1 attacttcgt ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta
       61 ttttctcact aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg
      121 ttcctactac aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg
     4021 ctcgtttgct ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac
     4081 atctgaatag ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac
     4141 gaatactgaa ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc
     4201 tgtctgagca accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga
     4261 agctaaagtt gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa
     4321 ttttcaagtt taccataaaa aaaaaagaaa tgcaataaaa ataaataaga agctcattaa
     4381 gatttttcta aattattcct cattataaaa tttcaagtgt ttttttattt tgagttttac
     4441 tctcccgtaa gcgctcatta cttgcttgtg atccattgct aaacattcag ccatgtctac
     4501 aaaatcaacg ccttgcatat aataatgaaa aattttagct tctatttttg aaaactggat
     4561 ttcgcaattt tcgattgctt cttttaataa aagatgctga atcgctaaag gttcttgcaa
     4621 tgtgatctct gctaaaaagt ctggtccttc tgtctctatt ttttggtcga ttgagacttc
     4681 atctttttga tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat agatttgatt
     4741 gcttataatg cttttataat aacttccaaa gctaacacct ttgctttttt cgtaagttct
     4801 caatgcctga tataaagcga tccttgcttc ttgttcccaa tcctcagaaa cataatttct
     4861 taaataatag gtgtttttca aacgtagtaa aagtggaaaa tatttttgat acagggcagc
     4921 aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca taaaataatt
     4981 cctccataaa ttcgtttatg gaggaatctt atcatcaatg attcatcgaa ctgattagaa
     5041 cgatatgtta tttatttttt ctcattaaat cgtccaattt ttgggacaat ttttttaact
     5101 gctcttcatt ccatggagaa ttacgtcgga aatcaataaa atgcatatct ttattttctt
     5161 gggctattgt tttttccgtt ttttttatcg tcttatataa ttcataagca gaagttcgta
     5221 atgctccttg ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc actgtcactt
     5281 gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat ataactgtca gccgtttcgt
     5341 cttctttcgt aaaaacaaca tccaactggt atttcttata attttgttgg atgcccggaa
     5401 ctagttgggc atcgaagaca acgatgactt ccattccttc atatttcgca taatttgata
     5461 aacgatgtaa aagagcttct cttgcatccg ccattttgtc ctgcttttta agctggacaa
     5521 gttccggcca cgaaccaatc atgttgtagc cgtctacaat caacagctgt tttttcatgg
     5581 tctactcccc ctgacgttgg gaaaaagcct gatacataag caaaccagca gcaacactgg
     5641 catttaagct ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc atttcttttt
     5701 tcaatcctgg actcattcct ttgccttcat taccaatgac taaagcaatt gatcctttgg
     5761 cattccattt ttggaagttt gtgccggtca tatctgtgcc aaatacccaa aatcctgttt
     5821 ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg acatgttcga
     5881 ctgccccagt ggaagtcttt gttacgatag gtgtgattcc aactgctcgg tgttttggta
     5941 tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca aaattgtgtg
     6001 gatcttcgat gctatccaaa atcaaaaaga aggggtcttc ttttttctct tttgttcgtt
     6061 caatcagttg gtcaagggat aaatattcat aaggtgtaat cgcaagaacg actccttgat
     6121 gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg aacccatttg actggcacag
     6181 cttgttcacg tgcgatttct ttcagttctt ctactttttc tcctcgtgaa tcttcttgaa
     6241 gaaagagttt attccctcgc ccttgttgta gcgcttctac tgcggcatgg tgcccaaaga
     6301 cgaaattatc ttctaatgct tcttcatttt gatcagaaac tgactgattt cgtccagttt
     6361 gctttttatt tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc gattgatgtt
     6421 tgtttgttct ttctttaccc atttttttca ccaactttct gaatacacca gcggatcaat
     6481 tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc ttcgaaccct
     6541 gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc tcgtatggct tttagcattt
     6601 cttcctctgc gataataaag ttcttcttct tctgtcaaaa gatgttcttc cagcatttgc
     6661 tgaatcaaaa aggcttgtgc attcgcagaa acataattcg ttgccgcacg atgcagctta
     6721 ttgggttttg tttctccttg atcaatcagg tggtcacgaa catagatttc gtaaatcgca
     6781 tctccaacat aagccaaggc taaaccgttc aattgtgtgt aatctctcat gcttctcttc
     6841 tccatcgggt tccttgcggt gtatcttcta ataagatccc tttttctttc aacaaatcac
     6901 gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt tcttcgatca
     6961 attgttcaat ctcttcatct aataactctt ctgctgtaaa taaaataccg aagatcgaaa
     7021 gccattgcga gaataaagct tctccttgtg ccaagacagc ggcagaaact ttcggctggt
     7081 ctagataagt gttcagccac ttagcaagct catagaccac tgtgatccca tttgctgcat
     7141 taaaatcatc gtccatttct tcgatgaatc gctgttctaa attttctaac aaagcgattt
     7201 tatccgcatc atctgccaaa ttctctgagg cagtttcttg gcggaatttc aaattgtcaa
     7261 acgtattctt cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt tcactgtagc
     7321 ggatcggacg acggtattgt gtggtcgcca tgaagaaacg caagacttgt ggatcaacgt
     7381 ttttgatcat ttcatgtaca gtgatgaaat tccctagaga tttactcatt ttttcatcgt
     7441 cttcgccaat cgttacatag ccattgtgca tccagtaatt agcgaatttt ttccctgttt
     7501 tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct tgcccgccac
     7561 catggatatc gatcgtttct cctaaatgtt ttgttgccat aacggaacat tcaatatgcc
     7621 aaccgggacg tcctttgccc catggtgaat cccaagaaat ctcgccaggt tttgcttgtt
     7681 tccataaagc gaaatctaat gggtcttctt tttgctgctg ttcttcccct gtacgctgac
     7741 ttgctccgac ttccagttca tcgatggatt ggtcacttaa tttcccataa ttttcaaatt
     7801 tacgggtacg gtaatacaca tcacctgaag attcataagc atagcctttt tcaaccaaca
     7861 cttgaataaa tgctaaaata tctggcatat gatccatgac tcttggatgc agtgtagcag
     7921 gctcgacatt caacgctttc gtatcttctt caaatgctcg gatgaaacga tcagcaactt
     7981 ctggtgctgt aatacctaat tcgtttgctg tacggatgat tttatcgtct acatccgtga
     8041 aattcgatac gaaatcgact ttatagcctc gatattcaaa atagcgtcga attgtatcga
     8101 aagcaatggt gctacgagca tttccgatat ggatataatt atacacagtc ggtccgcaga
     8161 catacatacc gacatgtccc ttttgtatag ggatgaattc ttcttttttt ctggtcaatg
     8221 tattgtagat ttttatcatt ctgatctcct cactttttct cctttgattc gaacgacttt
     8281 tgctggtact cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa cggcacctgc
     8341 accgattttg gcgttttttc cgatcgttac cggtcctaat atctgcgcat tcgcagagag
     8401 catggctcct tgtttaacag ttgggtgtct tttacccgtt tcttttcctg tgccgcctaa
     8461 tgttacgcca tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc caatgacgat
     8521 tcccattcca tggtcgatga acacgccagt tccaatcgtt gctcctggat ggatctcaac
     8581 accagttagg aaacgccaga attgagcatt gatcttcgcc aataaaaaga gccggtgctg
     8641 atataaaaaa tgagagaaac gatgccaaaa caaggcatga aaaccagggt atgtcaaaac
     8701 gatctcagct gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa tcgctcgggt
     8761 aaaccaactc atgactttcc actcctttcg taagaatttt atgtaggaaa tcatcttctt
     8821 ataaagaaaa gaagcgcctt ttaaataagt ttccttatca aaagacgctt tccgcgtggt
     8881 tccacttttc ttcataatca atgattatct ctgacagcta acgcctgtta tacgttcttg
     8941 gctactttgt ttcctataaa acagtttttc ctgttttctg tgtttcgcca agaccactcc
     9001 cagaggcatt tcggcaacca ttcattgata ccttgcacca cccggtatct ctcttgtaat
     9061 gatcagctgc ttactttttt ctgttcatcg tgtttagttc agtacttgat ttaaatgtgc
     9121 taatgatttt tcacgaccaa gcaattcgat cgtttcaggc aattctggac catgcatttg
     9181 tcctgataca gctacacgga ttggcatgaa aagatttttt ccttttacgc cagtttcttt
     9241 ttgaacagct ttgatggcag ctttgacagt tggagcatct actacttcca tttgttctag
     9301 ctgtgctttg aatgcgtgga gaacagtagg aactgtttca ccagcaagta cttcttttgc
     9361 tgcttcattc aatacaggat gttcattgaa aaatagcgta gacagatcaa cgatttctgc
     9421 agcatagctc atttgaggtt gatacaggct aacgatcttc ttcacccatt cgattgtctt
     9481 ttcatctggg ttttctgcta cacgtccatc agcgattaga taaggcagac acatgtcagt
     9541 aaatgcatct agatccattg ctttgatgta atggttgttt acccattgca gttttttcgc
     9601 atcgaaagct gcgggtgatt tacttaggcg ttttggatcg aacattttga tcaattcttg
     9661 gtgttcgaag atttcgtctt cgcccacagg tgaccagccc aataatgcaa taaagttgaa
     9721 cattgcatct ggtagatagc ctaattcgcg atattgttcg ataaattgca agattgattc
     9781 gtcacgtttg cttagttttt tacctgtttc actgttgatg atcaatgtca tatgtccgaa
     9841 ggttggtggt gtccagccaa atgcttcata gatcatcagt tgttttggtg tattggcaat
     9901 atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga ccgcaacagc
     9961 aaaattataa gttggcatac catcgcgttt ttgtatgaca aagtcgccac cgatattatc
    10021 ggattcgaac gaaatagcgc ctttcaccat atctgtaaat gcgtattctg tattgcgagg
    10081 cacacggaaa cgtacaacag attctagacc ttgtgcttct ttttctgctt gttcttcagg
    10141 tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc gttgtgcttc
    10201 ccgttctgct tctaattctt cttctgtgca atagcatttg tatgctcgat tgctagcaag
    10261 cagctgatca atcagcggtt gatagatttc tttacgttca gattgacggt aagggccgta
    10321 ttctccagga ttttcaggag attcatccca attcattccc aaccaagcaa ggttttccaa
    10381 ctggcttttt tcgccatctt cgatattacg tttttggtca gtatcctcaa tacgaataat
    10441 aaagtctcca tcattgtggc gggcaaacaa ataattgaat aatgcagttc ttgcatttcc
    10501 aatatgcagg tgcccagttg gacttggtgc atagcgtacg cgtactttcg tcattttctt
    10561 cctcttcttt caaactagac tttgactaac agcaaaaatt ccgtcagtta cggcttctca
    10621 aatatcattt atttaaagcc actcagcaca aattttacct tttctaaggt aaatagtaaa
    10681 gccaactggt tgaatttcct taccagttgg ctgaatttct tttttatttt gattgtttag
    10741 tctttttctc tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa
    10801 agatcatgcg tccagcagct gtttgaaggg cacttgttac aacgacttcg atacgttcat
    10861 tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg taggctactc
    10921 cttgttggcg ttctgtcccg gcttttacta ctagaacatt catcgtttca ccagggatga
    10981 cgactggttt tacggcattt gccaacgcat tgatattaag aacaggtaca ttttggaatt
    11041 cagagacttt attcaagtta tagtcattgg ttacaatgac accatctaaa agcttagcca
    11101 atttgatcaa tttgctgtct acttcactga tatcttcaaa gtctccatcg tacatttcga
    11161 cagatatacc ctcttctttt tgcaaggcat ttaggatatc taatccgcga cgaccacgca
    11221 cacgtttcaa actatcccct gagtccgcga tgtattgtag ttcgtaaaga acaaaattag
    11281 ggatcatcaa ggttccttct aagaatccag tctttgtaat atcataaatc cgaccgtcaa
    11341 tgatcacgct tgtatccaag attttatatt tatggaaatt ttctcctgat tttcgttcta
    11401 acagttgact ctcaacttga ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa
    11461 aaactttctt ccattcttcg attcgtgtcg ttcccattct caatcccaga tagcctaaga
    11521 tgatcatcag taaaatcggc aatacactat tgacgaatgg aatttccata ttgtaaagag
    11581 gggtcgagat cacaacacct aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa
    11641 gataagtcaa gctgacctca cttagttttt gttctacttg tttgataata gctgagatat
    11701 gtttggccaa cagcaaagat aataaaaaga aaataagtgc gcctaacagg ctgttggtaa
    11761 atgtattatt cagccatccg tttgactgtt gaccgattgc ctgccaagct gagggtaaaa
    11821 atgaaatacc caagcttgcg ccgacaatga tcatcagcaa agtaatgaca cgtttttgca
    11881 tcgtttttcc tccgtttcga caatcattta ttagttagcg gaacacacgc ttcaacgttt
    11941 cagccaaagt tgctactcca actatctcga ttccttttgg cgcttcccag ttacctaagt
    12001 tgtttttagg taggtatact tttgtgaagc cgagtttttg gacttctttg actcgttgtt
    12061 caatatgact gacacgtctg atttctccag tcagtccaat ttcaccgatg aaacattccg
    12121 ttggctgtgt tcctttttct ttatagcttg aagcaatact gacagcgata gctaaatcaa
    12181 tcgctggttc atttaatttt accccacctg cagcttttaa gtaagcatct tgattctgaa
    12241 gaagcaaccc agctcttttt tccagcactg ccatgatcag agatacccga ttgaaatcaa
    12301 gacctgttgt tgttcgtttg gcatttccga acatagtagg tgtgactaat gcttggatct
    12361 ctaccaaaat tggacgtgtc ccttccattg ccacaacgat agcagagcca gttgcaccgt
    12421 ctaggcgttc ttctaagaaa atctgtgaag gattagcgac ttcttccaat ccatgctcat
    12481 gcatttcaaa aatcccaatc tcattcgttg agccaaatcg gttttttact gctcgcaaaa
    12541 tccgaaaact atgatgttgt tcaccttcaa aatataacac cgtatccacc atatgttcta
    12601 acattcttgg gccggcgatc gagccttctt tggtaacatg accgacgata aaaatggcta
    12661 tcccatttgt tttagcgatt ttgagtagtt ctgcagtggt ttctcttact tggctgacac
    12721 ttcctgccac acttgtgatg tctggctgcg tcatcgtttg gatggaatca atgataacat
    12781 aatcagggga gatatgttcg atggcacgac tgatttcgtt catatctgtt tcagcgtata
    12841 gataaaattc tgtgttgata cttccgaggc gttccgcgcg taattttatc tgctctgcgc
    12901 tttcttcgcc ggatacatat aagacttttc cgcctatggc tgctaattgc tgagaaactt
    12961 gtaacagcaa agtggatttc ccgatacctg gatcgccacc aatcaagacc atagaccctg
    13021 gcaccacacc tccgcctaac acacggttca gttcttctaa ttttgttttg atccgaggtt
    13081 cttttttagg gacgacgtct gcgatttttg ttggttttgc tttttctcct gtcaaacttg
    13141 tccgagttct gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta ttccattttc
    13201 cgcaatttgg gcagcgtccc aagaacttag gcgacacata cccgcaattt tggcaaacaa
    13261 attgtacttt tgattttttt gccattgtac cgcctccttt tatcctaatc atgagacatt
    13321 aaatcaagct taagtatctt taacgtttta tttactggaa gaaccaaatc cgccttgacg
    13381 ttgagcagtc gtttgatctt gatctgcttt taaaaacggc aaaaagatcc cttggccgat
    13441 acgctcgcct tttttgatca ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat
    13501 atggccttca tttgcctcat tattgtaata atcactgtca atcacaccta ctccgtttgt
    13561 caacactaaa aaccgcttca acggattgct tgaacggtta gcaagctgca aaaattcatc
    13621 ttctcccatg taagctttga ttccagtagg cacgagggtt ggtttcaatt gtttttcatt
    13681 ttctgataca tcttttttca atccatctgc gactgtttcc aacccctttt tccagcctgc
    13741 tttccagata cttggaatca cgacctcttc tgctgcttca aaatcatagc cagcagcatg
    13801 atgtgtagcg cgctgcggta gattgatatt ttgatcttta tatttagaaa taacttcaaa
    13861 tcctcttttt ctcaccggtt ttcct
//
LOCUS       DBNIBO010000065        13865 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.069, whole genome shotgun sequence.
ACCESSION   DBNIBO010000065 DBNIBO010000000
VERSION     DBNIBO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.069"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..376)
                     /locus_tag="AC1Y77_001581"
     CDS             complement(<1..376)
                     /locus_tag="AC1Y77_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HIQ1575159.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEK"
     gene            455..868
                     /locus_tag="AC1Y77_001582"
     CDS             455..868
                     /locus_tag="AC1Y77_001582"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HIQ1575160.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(906..2054)
                     /locus_tag="AC1Y77_001583"
     CDS             complement(906..2054)
                     /locus_tag="AC1Y77_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIQ1575161.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2176..2772)
                     /locus_tag="AC1Y77_001584"
     CDS             complement(2176..2772)
                     /locus_tag="AC1Y77_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1575162.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2972..5614)
                     /gene="alaS"
                     /locus_tag="AC1Y77_001585"
     CDS             complement(2972..5614)
                     /gene="alaS"
                     /locus_tag="AC1Y77_001585"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIQ1575163.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6002..6433
                     /locus_tag="AC1Y77_001586"
     CDS             6002..6433
                     /locus_tag="AC1Y77_001586"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575164.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6478..7827)
                     /locus_tag="AC1Y77_001587"
     CDS             complement(6478..7827)
                     /locus_tag="AC1Y77_001587"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIQ1575165.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7897..9060)
                     /locus_tag="AC1Y77_001588"
     CDS             complement(7897..9060)
                     /locus_tag="AC1Y77_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1575166.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9189..9512
                     /locus_tag="AC1Y77_001589"
     CDS             9189..9512
                     /locus_tag="AC1Y77_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575167.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(9695..11044)
                     /locus_tag="AC1Y77_001590"
     CDS             complement(9695..11044)
                     /locus_tag="AC1Y77_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1575168.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11113..12066)
                     /locus_tag="AC1Y77_001591"
     CDS             complement(11113..12066)
                     /locus_tag="AC1Y77_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HIQ1575169.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12168..13358)
                     /locus_tag="AC1Y77_001592"
     CDS             complement(12168..13358)
                     /locus_tag="AC1Y77_001592"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIQ1575170.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         4058 a         2905 c         2375 g         4527 t
ORIGIN      
        1 ttttttcaaa atgatcaaaa aggactactt gatctttttt tcgcatcatg aaaacagatg
       61 gggctttctc atcttgcaac atcatttgca ccccagtatt agttggcgtc aaagatcgaa
      121 tctgtccgac agatatttct cggtctttcc aaaaagcagc ataacgtata atcacagagt
      181 ttgattttaa aataaaagaa cgccgcaaac ctagaaatag aaaaacaaga aaaataccta
      241 aaacaaaatt gcttttccaa taaggacctg tgttttctaa agaaagaatc agactaaaaa
      301 acagaatgat caaggtcaat gaccaataga tgatcgtatg tgccagttca ggctgccatc
      361 gaatattttt tttcattctc taacctctca ccgattcagt aatgatataa tcttaccata
      421 acattagttt gaacaaaaag gagaatctct ttttatgtta aaagtataca ttgatgcctc
      481 tacaaaaggc aaccctggtc ctagtggagg tggaatcttg attgtctatc aaggtaaaca
      541 agaacagcta tctatcccgc tttctatagg gacaaaccat caagcagagt tcgaagtctt
      601 tttaaaaact ttagaatttc taaagaaaaa cgacttacag aatgaaacga ttttctgttt
      661 ttcagacagc aaaacactag tttctacggt cgacaaaaac gcaacgaaga acgagctctt
      721 ccttccctac cttcaaagaa tccaagaact tctatccgaa ttttctttat tgatcctgca
      781 atggttgccc gaaaacaaaa ataaaggagc agataatctt gcacgtcagg ctctccataa
      841 acaattaaaa gaaaaaaaga ctctttaaat taaaaacttt aaggagtctt tttatagttt
      901 ctcatttaat ataaaagata attgtgatca aacgtaattt ccaattggta atctccgatt
      961 ttttcttcta tttgactacg taagattctt tctagttctg tatcaacaaa ctggaatttc
     1021 tcagggacca ctacatcaaa ttgcagcgtt tttccttgat gaattactct aaaatcatga
     1081 atattaagcc ctgtacctgt ttcaagaaga atttgcttta actgccgcaa gatattatag
     1141 tatttttcgt tatcaacagg cattggatcc aagtgacaga caagttccac atccaaagac
     1201 tttttaaagt ctttttctat cagatcaata atcttatgag ccacattcaa attcattgat
     1261 tcatctactt cgatgtggac tgatgcaaag cgtttattgg gtccatagtt atgcaccaat
     1321 aaatcatgat agccaagtat atcatcgcaa gcatttaaat gctcttccat ttcttcaatc
     1381 tctgctggcg ttgggcgact tcctaatagt tcgtatacaa aatttcgaag catcgtcaat
     1441 ccactataaa ggatatataa tgctaaagca aaacctatat atccatcaat acgccatcca
     1501 gttacccatt cgattgctgc tgaaagcaat acagccagtg tagtaaagac atcattataa
     1561 ctatctgttg ctgtagctct caatgtttca gaatcaatgg attgcgcaat aacctgatac
     1621 attttgcctt gtgcaaattt caaaaagatg gaaatgatta ggacaaaaaa gacgatgggt
     1681 gttaaagaga cattttctgg atggatgatt ttttcagcag aagactctaa aaattgaaat
     1741 cctacataag taatgatgat cgaaacaaat aacccactga tatattcgaa gcgttcgtgt
     1801 ccataaggat gttcctgatc tgctggcttt gcagcaattt taaatccgat caaagtcaat
     1861 atggatgaag cagtatctga tagactgttc actgcatcag ccataatgga gacacttcct
     1921 gaaaaaagcc cgatcattag cttagagaca aacaagatca gattcgtaac caatcctaag
     1981 atacccgcta gtactccttt ttttgtccgc tgttgcggta tttgtttctt tttttcttct
     2041 tgtttcttag ccaagttttt taccccttct agtttgcttg ttgctcaggt aaaaagatgg
     2101 agaggacgat tgttttcctc tcctctcttc ctttttctat ctatcgtgcg tcctattact
     2161 attaaaagat ggtcattatt ctggtttcaa cggtgttgct gcaaataacg cttttgctag
     2221 taatggtgtt atgattcccg cacctattgc ttctggtatc ccattgattg ctgcaacccc
     2281 tgctaaggtt ttaaataaaa gagcagagtc tacaccatag gcatctgccg ttgcacctgt
     2341 ataaaataat cccatgaatc ctaacactaa gactgtattg accaatgaac ccagtacccc
     2401 tgcaaaggct gagctgatcg ttattgattt atttttttga taaatccagt ggaaaaccaa
     2461 gcctgctact aagccaacaa gtactctggg aactaccgag ataatcggat tcgtgaacac
     2521 taatgtatca aatggcgttg ttggcatggc ataggcacga acaattgtag cgattcccca
     2581 aaacaagcca atcaccatgc catcttttgg gcctaatact actgcagcaa taattactgt
     2641 gatatggata attgttaaac taatgaatcc taatggaata aaccccagcc aaggaaccat
     2701 cgcttgtacg atgatgattg ctaacaagat cgcacgtaag actagtcgaa aggtcttatt
     2761 tctactattc attttttcac ccctttttat ttatactaat cgtaaaaatt tctgtcactt
     2821 tcgtcaacta aagaccatcc gaatccgacc atttgattag atgcgccatc caattgaaaa
     2881 aagtatgagg gcaagacttg tttgtcttcg cccccataat catgtttcgt tcaatgtttg
     2941 aacttttgtt tttctgtcaa acgtaaacgc cctattgttg cgcaacccat tctttcgcag
     3001 ctttaagtgc atctggaata ccagcaggat ttttcccgcc agcttgtgcc atgtctggac
     3061 gtccaccacc gccaccgcct actttcggtg cgattgcttt aatcaagtcg ccagctttta
     3121 gtccagcttc atttgattct ttcgtcattg ccgctagcag actgactttt ccttcttgtt
     3181 cgtttgctaa aacaagaata tctgaaatat ttttttgttt ccattggtca gctaattgac
     3241 gcagctgatt catatctttc acttttactt gtgctgcaat gattgttttt ccattgatcg
     3301 tttctggatc tttgaataca tcaccagctt gttgattcgc taacttgcta gctaattgtt
     3361 cattttcctt ttgtagctct cttacttgct gttgcaattg ttccgctttt gaaacaactt
     3421 ctttcaattg aggtgattta acaattgttg ctacttgttt tagttgtttt tcttcttctt
     3481 caagtagttc gtacgcttct ttacttgtta cagcctcgat tcgtcgcacg cctgcaccaa
     3541 ttcctgattc agaaacgatt ttgaagattc cgatgttttc tgtatttgct acatgtgtgc
     3601 ctccgcacag ttcgattgac cagccgccaa tatttactac tcgaacgtca tgtccgtatt
     3661 tttcaccaaa caaagccatt gcacccatgt ttttggctgt atcgatatct gtttcaattg
     3721 tttcaactgg gatagcttcc cagatttttt cgttgacgat tctttccatt tcagctaatt
     3781 catctgaagt gatttgacca aagtgggtaa agtcgaatcg taaatgtcca ggagcgacta
     3841 aagaaccagc ttgatttgca tgctcgccaa gtacttcttt caatgccttg tgcagtagat
     3901 gggttgctgt atggtttttg atgatccgat tgcgcatcga ttgatcgatc aatagatgat
     3961 aggtttgtcc ttcactcatt ggtgctttta cttctacggt gtgcataaat tgaccattag
     4021 gcgctttttg aacatctgta acaactgcta cgatttcgcc tacttcattc atgatccatc
     4081 cttgatccgc tacttgccca cccatttctg catagaaagg tgtttgatca aagatcattt
     4141 gagcttcacc ttctgcgact tcattcaaaa gagactcacc tttaacgatg accaataatt
     4201 gactatctga ttcttgttct gtgtatccaa tgaatttact ttctactttg atatctgtca
     4261 ataaagcaga ttgtaccccc attgattttt cagtactgcg ggcagagcga gcacgttcgc
     4321 gttgtgcatc catttctttt tcaaaaccag catgatcaac tttcagtcct tcatcttctg
     4381 ccacctcttc cgttaattct accgggaagc catatgtgtc gtatagtttg aagatgtctt
     4441 ttcctgctag agtcgtcccg ttttcgtttt ttacttcttg gatcacttga tttaagattt
     4501 ccaaaccttc attgattgtt tcattgaaac gttcttcttc tgtacggata actttttcaa
     4561 taaattcttt ttgttgaagt acttcaggat aataacttac catgatttcc ccaacaacag
     4621 ggactaaacg atataagaat gcttgttcaa tacctaattt tttcccatgc atgacagcac
     4681 gacgaagcaa acggcgtaag acataccctc tcccttcatt cgatggcaat gcaccgtcgc
     4741 caatagcaaa tgaaagggcg cgaatatgat ctgcgattac tttgtaagaa atatctgtta
     4801 ctgggttttc accatatttt acttgtttgc ttagcttttc tacggcgtgg ataataggca
     4861 taaacaagtc tgtctcaaag ttcgttgacg catcttgaac gattgagacc atgcgttcca
     4921 atcccatccc cgtatcaatg tttttatgag gaagtggttc aaatgtgtta tcaggcttat
     4981 ggttgaattc tgaaaatact aagttccaga tttctaaata acgttcattt tctcctcctg
     5041 gatagttttc aggatcgtct tctgctaaat cgttataagc ttctccacgg tcatagaaaa
     5101 tttccgtatc cggaccacat gggcctgcac cgatatccca gaagttgtct tctacatcaa
     5161 taatgtgatc tagtggcagg ccaacttctt catgccagat gcgttttgct tctgtatctt
     5221 ttggataaac tgttacataa agtttttctg gatcgaatgc catccatttt ggatcagtca
     5281 aaaattccca tgcccagtgg atggcttcat ttttgaaata gtcaccaatc gaaaaattac
     5341 ccagcatctc aaacatcgta tggtgccgtg ctgttttacc gacattttcg atatcatttg
     5401 ttcgaataga tttttgggca tttgtgattc ttggattttc tggcacaact gaaccatcaa
     5461 aatatttttt caatgttgca acaccagagt tgatccacaa taaggttgga tcgttcacgg
     5521 ggatcaaaga cgcactaggt tcaatcgtat gtcctttaga ttggaagaaa tcaagaaaca
     5581 tttgtcgaac ttgtgcacta cttaattgtt tcatgtcatt acctgctttc tataaattaa
     5641 atacaaaaaa acaccatcac agccaaggac gaaaactcgc ggtaccacct tgattgcaat
     5701 gatgtgtaat cattacctct taaactcgat aacgctgagc tgcgtcggta tttctaccaa
     5761 aatagaaaaa gggagcaatt tgattagtga ctattctttt ttcagccaag aaagaatttt
     5821 ctgtgagtct taaaaaacaa atcatatcct tttcgtactt atttagtata cgcattctat
     5881 tgatcactgt caatatctca atttttattt tcatttcctt ttggtcatac tccacgattt
     5941 ttggtacgat aagaataaag gaatcaatac atctctgcta ttcatgacaa ggaggaatat
     6001 gatgaaaaaa atagttggag cggatgaatg gatacgcgca gctagctgct ttttgagata
     6061 tcaagttttt gtattagaac aagggattcc tgcagaacta gaattcgacg gaaaagatat
     6121 cccgcaacaa acctattatc tatttcttga tcaaaaaaga ccagtagcta ctttgcgtta
     6181 tgaaaagaaa acacctactt gcttcaagcc ggaacgattt tgcgtagcaa aagaataccg
     6241 aggaaaaggg ttaggcggtc agttgcttga agctgctgaa aaacgggcgg tttcagaagg
     6301 ttgtcagact agttatgtag tagctgaaat gacggctgta gatttttatg aaaaacaagg
     6361 gtatcatact tgcacagaac cttttattga agatgaagta ctttgtatcg gaatgaaaaa
     6421 aagtctagct taaaaacaag agattgtgac acgccttttg tcacaacctc ttgtttttta
     6481 tttatgtgaa tttttcttat ttttacgagc agtacggctt tgctgtctgc gttcgatttt
     6541 gcgttctttt ttattttttt ctgcaattgc ccaatcgatt tttttcttat aaccaggctt
     6601 gatctttttc tttttcttct tcacaagacc aatcagcgtg ggatctaata catcttttga
     6661 tttttctcgt ttggcacgtc tattacgatc gtatgttttg acgatttccc cgtttttgat
     6721 ttcttttggt tcaaatgaga cgcccaattt ttctatcgca tcaatggctt gctcatcatt
     6781 tggcgaatat aacgtaatgg ccgttccttt taagccatta cgtccagtac gtccgacacg
     6841 atggataaag aagtctagat cttctggaat ttctgcatta ataacatgag agacaccttc
     6901 gatatcgatc cctctagcag ctaaatcagt cgctacaaca tattgatact ccagattctg
     6961 tacttgtcgc atcactcgtt ttctctctct tggggtgatg tctccatgaa tctttgctac
     7021 tttcaagcct tggtttttca agtaatcggc aatttcatcc acatgctgtt ttgtattggc
     7081 aaatacgatt gctaaataag gatgaccaat tgtcagcagt tgataaatca tttgattctc
     7141 atctttacct tttgttgaaa tcagccaatt ttcaatgtct tcagaaataa ctgcttttgg
     7201 tttgatttct tcaatgattg gattttccat gtattttcgt aagaatggac gaagtttttc
     7261 tggaatagtg gcagaaaaaa ctaaaaactg cagcttttct ggtaatcgac ttgcaatttg
     7321 gtctacttca tttaaaaaac ccatatccaa tgtcatatcc gcttcatcga caacaaatgc
     7381 aaatgcggtg tgaatcttca gcgcttgttc attcatcatg tctaatattc taccaggcgt
     7441 gcctactaca acctgtggct gttggtgatg cagacgattt aattgacgct gtttgtctgt
     7501 accgccgaca aaattcgaga cacggatctc cggtgtagaa aatttagcga tttgtgtagc
     7561 tgcttcatag atctgtgcag ctagttccct acttggagct gtaataacaa cttgtacttc
     7621 ctctttttct gcattgattt tttcaaacaa aggcaacaaa aacgtatgtg tttttcctgt
     7681 accagtttga gattgcccaa tgatattttt tccttttgca attaaaggaa tcaaacgttc
     7741 ctggacctct gtgggctgtt tgaagccctt tgcggttaac gcttcattga taaacggttt
     7801 gaagcggaat atatcaaaat tattcattcg tgcacctctt ctctttaaat tatcacgact
     7861 ttggcattat aacacagaca aagcgttttt gctcagctaa tttcgagaat catcatcctt
     7921 tgtttggcca atcgtgctaa cagtatatgt gtgactagct ttttcataaa acagaatccg
     7981 tttttttctc aaaaatgata gcaagatatt taaaataaaa ataccttgta gtaacaagac
     8041 accgagagaa atgaatactg cactcatctg caacaccgta ttcgttgtcg aattcatgat
     8101 ttgctgaagc aactgcccca accagcttaa accataaaaa agaaagtaca gatacccgta
     8161 gcggatcatc aatgctttta aagaaattcg ttcgcggtct tcttccacca aacggatacg
     8221 cacaattttt ttgccaagtg tccatccatt ggttataaac ggtacaatca taaagtatac
     8281 ccctacttct aataaaagcc accagatatg agaagtaact gcccctgaat cgaacgcaat
     8341 tttaaagaaa atcgccgcta ccaaattaat aagagataaa aatgtccagt cgatcaaata
     8401 agcgaccaag cgtcgagtca atgagacagt ctttcctttt tcataagaaa gttcatccat
     8461 tcgtcttctt gtaggaagca taaatgtaaa taacggcgta acgacaaaac caatgattcc
     8521 acccaatgta tttgtcacta aatcattcac atcaaataag cggtatggtc ttgggtagat
     8581 aaaataaagt ccacttagct gcgtcaattc aaaaaataga gaaaggcaga aactagcaat
     8641 catcgttttc ataaaagaaa gccggaaata atatcttaaa taaatgccaa atggtatgag
     8701 taataaaaca ttaaaagctg gttccaaaaa tacactctgc ttcattgccg gcaggtaggt
     8761 actaggttca gacaagacca ataccgtgtt gtctctgaaa tgttgccatg aagcaaataa
     8821 atgcagctcc atctttggcc ctgtcatttg ggcaaccgtc tcctttgccg gaagtggtaa
     8881 gatcaccaaa aaatacgcac acaataaata aaaaacgaaa gaatacaaaa taactgccct
     8941 tgttactaga aatgttccat actttcgata ttcataaata aaaaaaacag atgagatcgc
     9001 taaggctaaa aatggaaaaa caatcagcgc aaaacgaatc ggttcgatat aacttgccat
     9061 atgactcctc cttgattctt tgtatctctc ttatcttagc agtcctttgc tgtttcaagc
     9121 aagtaacgag gctattattt ctcatttctt tcaaagtaaa gtaagataaa cacaaagggg
     9181 gtgtcgagat gctgaatatg tactttgttt ttggcgtacc tattttttta ctgttccttt
     9241 atgctacgat cgcttatgta agaaaaagaa ctacaattca ttatttagga tttattctcc
     9301 tgattatttc gggtttcatg ctagtcttca atcttcagac ttggcagcaa gcacttcttg
     9361 aaatggataa aatgactcca catgctttgt ctaaagtgct tggctaccct gtctatctga
     9421 tttggcttcc cattttcatt tctggttgct tggttttatt aaatatttat cgcggcgtac
     9481 gccgaatagt tcagttacgc aaatcaaagt aaaagacaga aatcggttga gcccgatttc
     9541 tgtcttttac tttgctattt catcgtagac aaaacgacat ttacgtgatc aaaattaacg
     9601 agtacttttg acatttcttc cacgtactcc tcatttaaat aaaatatttt gtttggcaga
     9661 gaagtgcagt tctaataaaa ttcgaatgtt ttagctatct acgttttaaa tagagataaa
     9721 tcgggatacc agcaagtgtc aatactatcc ccatcgaagc taaaaaggtc tgagtgataa
     9781 gcgtgcttga tacgataaaa gtccctccaa tgattgctac tagtggaata aacggataca
     9841 ttggaacttt gtatggtcga aacaaatcag gttctttttt acgaagaaga atgacaccaa
     9901 caaagaccat cgtataaaaa atccaaatga caaagatcag catatccgtt agtgtatcaa
     9961 aaccaccgat catcatcatg ccaatagcga tgactagctc taagatacct gcggctacag
    10021 gaactttagt tttatcatga agtttagcaa agagcttgct aaaaggcagg ttgttttctt
    10081 ttgccataac ataagggaga cgcattccag taagggtata gccattgatg gttccataaa
    10141 cagaaatcaa gataccaact gtgaccagtc ttccaccgaa gccgccaaaa atcatttttg
    10201 caacgtcact tgccgcattg ctgtttcctg cgactccgtc gatagatgcg gttcttaaga
    10261 acaccgcatt tactaataaa taaacgatca tgattccgat gattcctaaa gaaattgctt
    10321 ttggcaaatc tttcgccggc tttttcaatt ctcccgaaat atttcctacg tgtatccaac
    10381 cgtcgtatgc gaacatcgtt gctagcaagc ccgctcccaa ggctgagaaa aatgaaagat
    10441 tttctcctgc ttggatcgga aacagttgga agtctactcc ttcttgtcgg aacaaaccga
    10501 aaatgacgat cacaaacaaa ggaataagct tgcagacaag cgtaatcgac tgaaaagccc
    10561 ctccagcttt agaacctaaa aaattgatca acaggatcga tacagcagct gtaacagcta
    10621 cgggaacaat cattgactgg gaaagaccaa atagattgac gaactgtgta ccgaaaatga
    10681 tcgacaaggc tgctacattt gctggaaaat aaatcacgac ttgcgcccag cccaataaaa
    10741 aagctgctgt atttccgtaa atccgttcga tatatttaat catccctcct gtttctggaa
    10801 tagcagctgc cagttcagct cctgttagtc ctgcacagac agaaatcata ccaccgagaa
    10861 accaagaaaa catatgcaaa ctggcggatc ctgtaacttc tgctacgctt gcggctttaa
    10921 aaaatacccc tgcaccgatc actgtgccca taacagtcga taaagcagta aaaaatccca
    10981 tcgtacgttt taattcattt tctattttta cttcttttac tggcgtggct tgatattctt
    11041 ccatcccttg tcccctttca taccatagcc aatcccaaaa gtataaaaaa ctttttggat
    11101 gtggttcttg ttttataccg tctctaccaa tttttctttc agtttttcca acatatcctt
    11161 tgtcatcaca tctaagtcgt atttcggcga aaagccccat tcctcttgcg cacaagaaac
    11221 atctagtgaa tttggccaag attctgctat tgactgacgt acaggatcca catcataatc
    11281 catttcaaaa tctggtatga ttttttgaat agatgctttg atctcttccg ggtcaaaaga
    11341 catcgccgat atattgaagg catttcgatg aaccaatcga tcaggatctg cattcatgag
    11401 ttttataatg gcatcgatcg catctggcat atacatcata tccattgatg taccttgatc
    11461 aatgaaacta gtgtactttc cttctttgat tgctgaataa taaatatcga ctgcgtagtc
    11521 tgtcgtaccg cctcctggca aggttgcata agaaatcaat ccagggaaac ggacaccacg
    11581 agtatctacg ccatatttca cataataata atcacatagt agttctccag ctaccttcgt
    11641 taccccatac atggttgtag gacgctgaag agtatcttgt ggggtatgat cttttggagt
    11701 atttggacca aacgcgccga ttgaacttgg cgtaaaaaat ttcagctgga attctctagc
    11761 gacttccaat gcattgacca gcccagtcac gttcaaattc caggctaatt gtggttttgc
    11821 ttctgcaaca gctgacaaaa gagcagccaa atgaatcaat gtgtccactt gatattgttc
    11881 tgctatgctg cgcatttttt cgtaatccat tacatctagg atctcaaaga tatcgttttt
    11941 ctcaatgact tcaggcatgc gtatatctgt cgcaatgaca ttttcactgc ctagttcttg
    12001 acgtagccgt gtgatcaact ctgaaccaat ttgtcctaaa catcctgtca ccaatacttt
    12061 tttcatgatg atcctccctg atctttttta ctactatctt cagatgatta acaatatatg
    12121 cgtgaacatg ttctctaatt ttaaatagac gaaaaaacta aatttagtta aataatttcc
    12181 atttcttttc ctattttttg ataaactgct actgcttcat caagcatttc cctcgtatgt
    12241 tccgcagtcg gcatatttct gatcctgcct gtcccaagag gcacagttgg ataaacgatt
    12301 ggtttagcat aaatcccatg ttcgatcagc ttcttagaga aagtctgagt cactttttcg
    12361 tctcccaaaa tgactggagt aataggtgtt tcagacatac cgatgttata gcctactttt
    12421 ttcaattctt ctttaaaata attagcattt tcccaaactt tttcgacaag ttccggatgt
    12481 tcttgcatca aagtaatcga agccaatgca gctgcagctg cacctggagt tagcgaagta
    12541 gaaaatagga atggccgact tctagctttc aaccagtcga ttagtgtttt cgatccagct
    12601 acataacccc caacaacacc tatagctttc gataacgtgc ccatttgcat atcgattttg
    12661 tcgcttaatc caaaatgctt gacggttcct gcaccttttc cagttacgcc tgagccgtgc
    12721 gcatcatcga cgtaggtgat cagtccatat ttttcaacaa tcggaataat ttccggcaat
    12781 cgtgcaatgt ctccatccat ggagaaaaca ccatctgtga tatacatgat ttttttatat
    12841 ttcttacttt ctatcgcttc ctttgctttt ttctcaaggt ctttcatatc ttgatgttta
    12901 attcggatga tttttgctcc ggataagcgg caaccgtcaa taatagatgc atggtttaat
    12961 tcatccgata agatagcatc ttcttttgtc attactgcag agatagcgcc catattgcag
    13021 ttgaatccag attgaaaggc gattgctgct tctgtccctt tgaattctgc gattttttct
    13081 tctaattgct gatggatttt cagcgaacca ttgatcgttc ggactgctcc tgctcctaca
    13141 ccataagttt cggttgcttc aatacatgct tttttcaatt cttcgcgatt ggcaaatcca
    13201 agataattgt tggatgctag attgatcatc ttttttccgt ctacgatgat acaagcacca
    13261 ttttcacttt ctagtatgtc gatcgtattg tataacccct tttctttcag ttgatccaat
    13321 tctttctcta aatacattgt tagttctttg gaagacattg tctccacctc catattcatc
    13381 tgtcttgcag aacataccaa acatatatcg tgtcattcat ctacaaagca agtatgctat
    13441 atttttgata acgcttacac tgattatgag aattatttaa ccacattttt tatttacagt
    13501 atctttttac tttcaaatga cttatatagt atgaacaatt accttttaga taatgatcta
    13561 ttttactcta cctgggcaga taaatacaac acaaccattg atagaagccc tttcgttaaa
    13621 atcaattgta ttctgaatat ttaatttatt tattgcatag accaataaaa atacataaaa
    13681 tttccatctt cgtttatcag caatttttaa ttttactctc tgaatatttc ttaaaagttt
    13741 tacttgttga attcgaaaaa aggactgtga caaaagtctt gtcacagtcc tattcttccg
    13801 aataaacggt gttctcctgc caattccgaa tcacaagtta caatattcat caaacacgag
    13861 aagct
//
LOCUS       DBNIBO010000066        13864 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.070, whole genome shotgun sequence.
ACCESSION   DBNIBO010000066 DBNIBO010000000
VERSION     DBNIBO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.070"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(105..425)
                     /locus_tag="AC1Y77_001593"
     CDS             complement(105..425)
                     /locus_tag="AC1Y77_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1575171.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECFFIYDI
                     IASK"
     gene            726..2606
                     /locus_tag="AC1Y77_001594"
     CDS             726..2606
                     /locus_tag="AC1Y77_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1575172.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2685..2912
                     /locus_tag="AC1Y77_001595"
     CDS             2685..2912
                     /locus_tag="AC1Y77_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIQ1575173.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2917..3459
                     /locus_tag="AC1Y77_001596"
     CDS             2917..3459
                     /locus_tag="AC1Y77_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIQ1575174.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3710..4387
                     /locus_tag="AC1Y77_001597"
     CDS             3710..4387
                     /locus_tag="AC1Y77_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HIQ1575175.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4729..5949
                     /gene="sstT"
                     /locus_tag="AC1Y77_001598"
     CDS             4729..5949
                     /gene="sstT"
                     /locus_tag="AC1Y77_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIQ1575176.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            6096..6731
                     /locus_tag="AC1Y77_001599"
     CDS             6096..6731
                     /locus_tag="AC1Y77_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIQ1575177.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6922..7665
                     /gene="truA"
                     /locus_tag="AC1Y77_001600"
     CDS             6922..7665
                     /gene="truA"
                     /locus_tag="AC1Y77_001600"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIQ1575178.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7899..8732
                     /gene="msrA"
                     /locus_tag="AC1Y77_001601"
     CDS             7899..8732
                     /gene="msrA"
                     /locus_tag="AC1Y77_001601"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIQ1575179.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8846..9806
                     /locus_tag="AC1Y77_001602"
                     /pseudo
     CDS             8846..9806
                     /locus_tag="AC1Y77_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            10095..10793
                     /locus_tag="AC1Y77_001603"
     CDS             10095..10793
                     /locus_tag="AC1Y77_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIQ1575180.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10943..12259
                     /locus_tag="AC1Y77_001604"
     CDS             10943..12259
                     /locus_tag="AC1Y77_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1575181.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            12276..13691
                     /locus_tag="AC1Y77_001605"
     CDS             12276..13691
                     /locus_tag="AC1Y77_001605"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1575182.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
BASE COUNT         4519 a         2247 c         2926 g         4172 t
ORIGIN      
        1 cattagtttc aatgtttact gagtaactaa ttattttgca ggaggacaat ttatttacac
       61 aaaattattg acgctcccaa aatactcgtt tacataaaaa aagattattt agatgcgata
      121 atatcataga taaagaaaca ttcatcataa gaaacagtga tcccaaattc tgtttcaata
      181 tttttgaagg ccttttttac atgaaaaatt gtttcatgag aaggtcttgt gtcatcttct
      241 gatataattt taagttcatg accgtttatc aatgtacgct caattagaca cgctgtatga
      301 atcaaaagtt ttaaaagaat ggaatgagtg aattttttgt ggaaagtaca ttccaattct
      361 tggataaaat ccattaagct tttttggatc ttattcccag ctaataagta gagatatttg
      421 tccactgctt caactcccat atcaaaaaac gatatttctt gtgaatgagt ggctgctgcc
      481 cttttttgat tgttgttctt cagtcgtaag atgacaaagc atcttatctt tgaataaaaa
      541 aatagaacta cctaaggaag cgtttcggcg cttccttttt ctatatgatc gtttaatgac
      601 tgtaggaatt cagtatacca aaagaaaacc gcctttttga ataaacttga tagtggtcaa
      661 ttttttatga tggataagta tctatactta gtctcgtaag aatgaaaagg aaaagaggga
      721 gaaagatgaa attacaaaag tggatccaag agcatcgaaa tcatatcacc gccttatcag
      781 ctgtgttgat tatttttggt tttatcggga aatttactgg atttgtcttg ttctataaca
      841 ttgctctagc catagcttct attattgcgg tcatacctat tataatccat gcgtatcagg
      901 cagtaaaagt caaagttatc tcaattgatt tactcgttac tattgctgtg cttggtgcct
      961 ttatgatcgg agaatacaag gagtcagcta tcgttacatt cttgtttttg tttggtagtt
     1021 atctggaaca aaaaacattg accaaaacgc gggaatcaat caaggctttg accgagatgg
     1081 ccccatcgac tgccagcgtt gtccaagaag atggaacgac cgaagaagtg gaaatcgatg
     1141 acgtggaaga aggcgatatc gtcttagtaa aaactggtgg agctattcct gtggacggac
     1201 gagtcattga cgggaaaggg tatgcggatg aagccgcagt gacgggtgaa tcacgacaag
     1261 tcgtcaaaat cagtggagat aacgtatttt ctggaacgat tttagcagat ggctacgtga
     1321 cgatcgaagc aactaaggtc ggagatgata cgacctttgc caaaattata gaattagtcg
     1381 aagaagcaca agataccaaa tcacatgcgg aaaaattcat tgatcaattt tctcagtatt
     1441 atactccaac cgttttactt attgcgttgc ttgttggtct gatcagtcgt gattttcgtt
     1501 tagccatcac tgttttggtt cttggttgtc ctggcgcttt agtcattggt gcgcctgttt
     1561 cgaatgtagc aggaatcggt aatggagcga aacggggcat cttagtcaaa ggcggtgaag
     1621 caatggatac tttttccaaa atcgatactt ttgtctttga taaaaccggt acattgacca
     1681 aaggaactac tgctgtatcc actatcaaga actatacaga ggatgaaggg gacagcttag
     1741 ctttagtggc tcgattagaa acgttatccg atcatcctct ggggcgcgcc gtgatccatt
     1801 atgctcagga gaaagacatt gattttcaac aattagcagt caaagataat cagacgatca
     1861 agggacaagg aatcacagct gagattgatg gtcatacagt ttgtgcgggc aacaccaaat
     1921 tgattgccgc tcacaaactt tctctcacac caaagcaagt acaagattta catcaattac
     1981 aacaaagagg cagctcagtc attattgtag caatcgacaa aaaaatcact caaatcattg
     2041 gtgtgtcaga tgtgattcga ccagaagtcg ctgaacaatt agctatgttg aaacaagcag
     2101 gagcaaaaca tttagtgatg ctgacaggtg ataatcagat gactgctgat tatgtagctg
     2161 acatgttagg cattgatgaa gtccatgctg aattattgcc agatgaaaag gttcaatttg
     2221 tgaaaaaata ccaagaacaa ggattgcacg ttgctttcat tggagatgga atcaatgatt
     2281 ctccctcttt ggctgcggct gatatcggga ttgctatggg atcaggaacg gatgtcgcta
     2341 tcgaaacgag tgacgttgtc ttgatgcaat ctagttttgg atcgttggtt catgcttacc
     2401 gtttggctaa gaaaactgtt ttgaataccc gtgaaaatgt agtgattgcc attggtgtcg
     2461 tcttattttt actcatcggc ttatttgccg ggtttattta catggctagt gggatgttcg
     2521 ttcacgaagc tagtatcttg atcgttatct ttaacgcgat gcgtttattg tattttggac
     2581 acgaaagcaa agaagaaaaa tcgtaaaact tgataagaat caattttttt atggaagctg
     2641 taatctagaa taagactatc aagaaaaagg aaggaagaac tacgatgaaa aaaatgatta
     2701 tgcaattaga tacactagct tgtccaagct gtatgcaaaa aatagaacat gcggtttctc
     2761 aacagcctgg ggtaagcaat acgaaagttt tgtttaatgc gagcaaaatc aaagcaaact
     2821 ttgatccaga tgaagtccac cctgaacaat tagctgcagt tgtggaaaaa ttaggttatg
     2881 aagtccaatc aacaaaagta aaagaggcgt aaatcagtga atatagtgga agaaaaattt
     2941 gctcaagaaa caaaacaagc agatatcgat catcataaac caactgctgg cgccatggtg
     3001 gggcatgtat tggcaaattt taaattagaa gaaatgaaat tgatccaagc acaattgtat
     3061 gttttgggta catcttcgca agaattaagt accctttttt cagaatatgc tgatcaagaa
     3121 gcgagatggt tcaataaaat ctctcatgag ttagtgattg aaaatgaaat cgttccgtct
     3181 actttagaag aagtcatgcg ttatggaaaa atttacgaac atggtcaaaa caaatattta
     3241 ccagcctcaa caatgttgga aaactttgca aaagattttg atttccaaaa tgtctttatc
     3301 acacgggcaa tcaaattagc tgaaaaagaa gaaaaatatg gcttgaaaca attgcttgtt
     3361 gaacttttaa gtttcaataa agaaatcatc taccaagtac aaagactcca aggaaaaact
     3421 gtccgacaag atttggatga ggaagacgat gatgactaaa agtctatgtt tcttcaaata
     3481 caaatataaa gcgtgaggta aattggaaaa tcgcgaagat taaaataaaa gaagcacccg
     3541 ctgatgtatt acagacaaca tcagcgggtg cttcttagtt tttaaaaggt ttaaaagatg
     3601 gatgagaaaa tacaaaaaat caataaaatc acattgctta tttgatagaa taaagaagtg
     3661 ggagtaatcg aaaggagttt tttaatttaa aaaaaggatt ttagtaacaa ttggtctagc
     3721 ttttttgtta gctggttgta ccaacgggaa tgaatcgaaa aaagaatcaa caaaagaaac
     3781 agcgaaagca acaattgcaa ctactacaac aactagcaca gagagcaagc caactgcagc
     3841 aagtacggaa acagcagata cctctacttc gtcagagtct tccaaaacga ctgaaacaac
     3901 tcaaacatct acttcaagtt cttctgtaga ggcttcatct tcagaaagca gcgaacaagt
     3961 cgaacaaaca ctgtggaatg cagacaaatc cgcacaatta gaggcattta tggctacatg
     4021 gggccaaaca atgggacaac aatacaaaag ctatacgaat caaatgagtg tggacttata
     4081 tggtcttaaa gtccctcaag ttattttgaa cggggagtgg aagatggcta ttggtggagt
     4141 gcctgtttct gcagaatggt cagaaagtgg gacaggtcaa gcagactatc aaatcacagc
     4201 cgtctattcc gatgcagaga cagaaccata tctgaaaaag cacgtctatc tattcgggtt
     4261 tcagcaaaat cagccaaaag tattggtgac acaacaaaat caggggaatc ccgataatta
     4321 tctttacttt aacgagacag cgaataatga attgaaaaat ggctttaatc aaatcgttta
     4381 tgggtaagta aaccagtgag agtgagagaa agaagagact cagtggaaaa tattaattga
     4441 ttgctatgtc taagaattaa gatgaaggat gaaatttcta gtttcgtcct tcatcttagt
     4501 tacgttttac ttggataact ataataataa cgataaatag ttttctctcg acttctttag
     4561 agattgaata aaccctccaa tactaaaatt gaatgttgca agagcatgaa ttgccaattg
     4621 tattattaga atttttagaa aattgcacct ttagtttctt tagataaaat gatacaatga
     4681 ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat gtcgttagtt
     4741 caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt ccccacgtgg
     4801 acatttattt caatattggg gacgttgttt gtcagttgct tgaaggcaat cgcacctatt
     4861 ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa taagacattc
     4921 gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact tgtagcagtc
     4981 attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc tgaaaaagca
     5041 ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca aaatcctgtt
     5101 caatctctta ttgatgcgaa ttaccttggc gttttattct gggctgtatt gttgggacta
     5161 gcttttcgag cacgttcaga accgacaaaa gagttggtgg atcagatatc tatagcgttg
     5221 tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg attggtttat
     5281 caatcaattg ctacgactgg aatcgctgga ttggcagagt atctgcagct tttacttgtt
     5341 ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac gtttttgttt
     5401 attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc acttcccgct
     5461 ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc tgaaagattg
     5521 aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat caatatgggc
     5581 ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt agggattgaa
     5641 gcaccatttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc ttgtggagct
     5701 tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt gtttggtatc
     5761 tctaatgatg tcgcgatgca aatcgttggt attggattta ttatcggtgt tatccaggat
     5821 tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat aggagagcta
     5881 ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga tggattaagt
     5941 gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta atgagttata
     6001 tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgttt ataataacaa
     6061 ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt aaatttggtt
     6121 ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta ttaggattgt
     6181 attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag gtttccagtc
     6241 ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc atcctgtcat
     6301 tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc ctagttacag
     6361 cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc agtatcaata
     6421 cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca ggtttaggtt
     6481 acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa aatgggcaaa
     6541 tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt gcggcgattt
     6601 atggactggc aagaattcct tctataaatt cttggccatt actgaataaa ataggttttt
     6661 gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt atcttgcatc
     6721 tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca tccggtggct
     6781 gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt ctgtcttaga
     6841 gaagcaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag accaaatgga
     6901 agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata tgacggaaaa
     6961 agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg aaaaatcgaa
     7021 aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg aagaacagat
     7081 gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga aattggactg
     7141 gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat caaaagaatc
     7201 gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa atacagctat
     7261 tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt ttatttccct
     7321 caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg tacacatgat
     7381 ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac gattgatgaa
     7441 ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga cggattctta
     7501 tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg tgccatgccg
     7561 atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg agaaaccgtc
     7621 cctgcacaag gtctgttttt ggatgaagtt tattatcgct cataaaaaag aatcatcttt
     7681 actcgacttc tgagaattgg agaacatccc ttttttcaga ggtcgttttt tagttaaacc
     7741 aaaccataga gtatgctttt taagataaag atcatcttat atcatctgaa gctgcttatc
     7801 ttctttgaat aaatacgatt caatcgatta tttcctcact ttatgataaa ttacagctaa
     7861 agtcactagc ttgtcttctt cggaaaggag gagaatctat ggatgaggaa caaatattca
     7921 ttcaaacact ttataatctg attttgaatc cgaatacacg agattgggaa cgaaaagtat
     7981 tgattcagac aaagaatgac acaagagaaa atatatccgt taaagaacag ttatcaaaac
     8041 tagaagtaac actacgtcca ttagctatcc gaatgaatct gacgcctgat gtaatggact
     8101 tttatttatt gattacggaa ggatttgata aggagcaaaa atatgacttt tccaagcatg
     8161 ccatgcaaga tgctgactat caagaacgtg cagtatttgc agggggatgt ttctggtgta
     8221 tggtcgagcc gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga tacacgggcg
     8281 gacatgttga aaaaccgaat tatgatcaag taagtggtgg ttacacaggt catgtggaag
     8341 ctgtggagat catttatgac acaagagaaa taagttatag cgagttgctg acaatctatt
     8401 ggcaaattac tgatcccaca gatacatttg gacaatttca agatagaggg aagcaatatc
     8461 gtccagtgat tttttatcaa gacgaacgac aaaaagaatt agcggaacaa tcaaaacaga
     8521 aactagacag ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa ccagccggta
     8581 cgttttggcc agcagaaaac tatcatcagc aattttacaa aaaacaaccg aaacgctata
     8641 aaaaaatcca gcaagcacgt aatcaatttc tgatctatca acgagtaaaa aacaaatggc
     8701 aaaaaaatat acgaaaaaat cattttgatt aactcctttt ctttaaaatg tagtaaacta
     8761 aaaatagaaa acaagaaagc gttttaaaaa gatttgatga ggaggagaaa aattgtatat
     8821 tgcagatagt gcacgttatg aaaaaatgga atatcgtaaa tccggtcatt caggtttaaa
     8881 attaccgatt ttatctctcc tctcggatta tggcaaaatt ttggtgatta tgatcctatc
     8941 cataatcaaa gagagatttt aagaggcgca ttcgacatgg gaattaccca tttcgacctt
     9001 gcgaataatt atggaggccc tgctggagct gcagagaaaa actttgggcg tatttttaga
     9061 gaagattttc aagcctaccg tgatgaactg attatttcta gcaaagcagg ttatcatatg
     9121 tgggaagggc catatgggga atggggctct cgtaaatcca tcatatcaag ttgtgaccaa
     9181 agtttccaaa gaatggggct agattatgtt gacatcttct atcatcatcg cccagaccct
     9241 gatacacctt tggaagaaac agcagaagcg ttgatgcaac ttgtaagaca aggaaaagca
     9301 ttatatattg gtatatccaa ctataatgga gaagatacaa aaaaatgact gaaattttaa
     9361 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     9421 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     9481 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     9541 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     9601 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     9661 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     9721 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     9781 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     9841 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     9901 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaattttatc ctagtctttt
     9961 ttatggtaaa tatcttcttc gtttgtatgt ctatgaacgg attaatcaat tttgtataca
    10021 aaaaatcatc agatgattca aacgtgttat aataattacg aaaaaataca tcagatgaaa
    10081 aggaggtatg tggtttggaa gaaaataaaa ccctagtaga agtaactact gacaagatcc
    10141 ttagatattt aaaggaaatg cagttgcaag taggagataa actgccgaat gaatatgaat
    10201 tgtccgatta tttagaggtg gggcgaagca cggtgcgaga agctgtgcgg gctctagctt
    10261 ccaggaatat ccttgaagtt cggcaaggag cggggacatt tgtcagtgat aagcgaggga
    10321 ttgccaatga cccactcggt ttttctttga ttgaagatca ggatcggatg atacgagact
    10381 tatttgaact gagatattta ttggagccta agatggcagg tttggcagcg gaatatgcaa
    10441 cgaagaatca aatcgtccaa atgaaaaaat taacgcaaaa gattgaaact tcttttcgag
    10501 aaggcacaca agaacatgtt caattagaca tcgattttca tacgatgatt gcagaagcta
    10561 gcggaaacgt agccttgctg catattatcc caatcattaa tgaatcaatt gctttgttta
    10621 ataaaaatta tgatatccag catctgaaaa tcgagacgat cgatattcat acagagatcg
    10681 ttcaggccat cgaaaggcgg aatgcacttg ctgcgtttga tgcaatgact gtccatatgg
    10741 caataaatcg gagagaatta cagaaaataa taggagaaaa agaaactaat tgacagttca
    10801 tctgatgtat tttatgataa atacatcaga tgaatttttg ttttatattt ggtaaaaaag
    10861 aggagtaaat agtttctgtt catcgaaatg taagcgctga ctaatagaca aaaagagatg
    10921 acgtcaagga gaagaagcag gaatgagggg atctggaatg agttttatgg ataaattagc
    10981 caatgtttta gggaacgtgg caacgagaat caataattta aggtatatca tggtgataaa
    11041 aaacgcattt gctgcattga ttccggtaat tattacgggt gcttttggca cattgttttc
    11101 agcaatggtc tttgactcgg aaaatggctt agcacagata agtttcttgc gttttttaga
    11161 agaattaaag cctatcgcat ctgccatcag ctatgtcaca ctaagtttct tgaccatcta
    11221 tgctgttttt ctgataggga tcgaattagc caagctgaat aaagtggatg gtgtattccc
    11281 tggtatcatc gcagtgatgt cttatttatc tgttaatcca actgtttatg aattagtgaa
    11341 tgaaaatcag actgttatag tagaaaacgt attggctaaa caatatacag atacgaaagg
    11401 gttatttttg ggaatgttgg tctccatcct ttctattgaa ctttattgct ggttgaggaa
    11461 acagaagaaa ctacagctga gaatgcctga taccgtgcca acgaatgttt ctgctagttt
    11521 ttctgcttta ttcccgacga ttctgactgt atcgggtatc gcggctgcgg gatttatcat
    11581 caaagaactt acaggaatgt acctttatga tatcatttac aatgtcgtac aacgcccatt
    11641 agaaggtgtc gtacaagggt taccgggtat tttgctattg atgtttattg cgcaagtctt
    11701 ttgggtgatt ggtatccatg gaaatcaaat ggtgaagccg atacgggaac cgttgctttt
    11761 agcggcaatt gctgtaaata cggaagcttt tgaagctgga aaagaagtac cgaacattat
    11821 tactatgcca ttttgggata tgtatatgag tatgggcggg tctggagtca cgatcggttt
    11881 actgattgca gtattgttag ttggaaaacg ggaagatatg aaacaaatca ccaagttatc
    11941 ccttgctcct gggatcttca acatcaatga accagttatt tttggtatgc cgatcatgct
    12001 gaatccaatt ttagctattc cgtttatctt aacgcctctt atcactggaa cgattggtta
    12061 ttttgcaacc gctttaggat ttgcggcgaa agcagttgta atggtccctt ggccaatgcc
    12121 gccgatcgtc aatgcttatc tggcaacagc aggaaatatc ggtgcagtta ttacgcaact
    12181 tgtttgtatt attgtttcta ttttgatcta tctgccattt gtaaaagtag caaatcgtgt
    12241 ggaaaatgat gggatctaaa aaacaggagg aaatcatgaa aattactatt ccaaaaaatt
    12301 ttattcttgg tgctgcatct tctgcttggc aaacggaagg ttggaaagga aaaaaagaag
    12361 gacaggattc gtttatagat agctggtata aaaatgaaca ttttgtttgg cataatgggt
    12421 atggcccagc cgtagcaact aattttatgg aacaatacca gcaggatgtg gatctgatga
    12481 aatccatcca tctgacacat tatcggactt ctatcaattg ggctcgtttt tttactgatt
    12541 atgagaactg tatagtagat gaagaatatg ctcagcatat aagtgaaatg atcgactctt
    12601 tgatcgaagc agacgtagaa ccgatgattt gtttggaaca ttatgaagtc ccagcttttc
    12661 ttttggaaaa atatgatggc tggtcttcaa gaaaagtagt tgatttttat gctaagtatg
    12721 ctcaaattgc ctttgatcgt tacggtgata gggtaaaaaa ctggtttaca ttcaacgaac
    12781 caatcgttcc ccaaacgcgt tgctatttgg atgctgttcg ctggccgcat gaacaaaaca
    12841 caaaaaagtg gatgatgtgg aattatcata aagttttagc aacggctaaa gcagtagaga
    12901 tttaccgcag cggaaattat ggtggaagga ttggctgtat tttaaatcct gaaatggtct
    12961 atgctcgatc caattctcca gaagaccaaa aagctgctta tatgtatgat ttgttttata
    13021 atcgagtatt ttttgatccg atggtaaaag gatactacac agaagagttc ctggagttgt
    13081 gtcaaaaaca atcgatttat ttcgatcctg caacagagga tttagaaacg attcagcatc
    13141 atacagtaga tttccttgga atcaatcaat attatcctaa acgcgttcgc gcccctcgtt
    13201 atcaatggaa tgaagagaca cctttccatc ctgaacgata ttatgaagtc tttgatttac
    13261 ctgggaagaa aatgaataat tccaggggat gggaaatcta tccgaaaatc atgtatgata
    13321 tagccatgta tctaaaagaa aattatcata atatcccttg gctgataaca gaaaacggta
    13381 tgggaagaga atatgaagaa cagtatatgg atgagaaagg aatcgtgcaa gatgattacc
    13441 gtatcgaatt tatcggacag catttgcatt acttgatcaa agcaacagaa gaaggcgcaa
    13501 attgcgaagg ctatatgtta tgggcattca ctgactgtgt gtcaccaatg aatgcattta
    13561 agaatcgata tggattagtt cgaatcgaat tgaacgatga acgaagcaga acattgaaaa
    13621 aatctgcttc gtggtatcag actttgattg ataagagaga attggatttg cctgacgaaa
    13681 tctataaata aatagaagtt ttttgtagat gttcattgta tttactggtt atttgaaata
    13741 aaagaggttg tgaatccagc gttttgagct aatacccagg gtcagctgat tgaacaccgt
    13801 ttattgagag gttgtgaaaa agctgtcctg catcaagtaa taagaatagc aaagtaaaaa
    13861 caac
//
LOCUS       DBNIBO010000067        13809 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.071, whole genome shotgun sequence.
ACCESSION   DBNIBO010000067 DBNIBO010000000
VERSION     DBNIBO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.071"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="AC1Y77_001606"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="AC1Y77_001606"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIQ1575183.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="AC1Y77_001607"
     CDS             complement(2262..2513)
                     /locus_tag="AC1Y77_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HIQ1575184.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="AC1Y77_001608"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="AC1Y77_001608"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIQ1575185.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="AC1Y77_001609"
     CDS             3463..3681
                     /locus_tag="AC1Y77_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575186.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="AC1Y77_001610"
     CDS             complement(3970..4482)
                     /locus_tag="AC1Y77_001610"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIQ1575187.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="AC1Y77_001611"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="AC1Y77_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIQ1575188.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="AC1Y77_001612"
     CDS             complement(6935..7378)
                     /locus_tag="AC1Y77_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HIQ1575189.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="AC1Y77_001613"
     CDS             complement(7379..8167)
                     /locus_tag="AC1Y77_001613"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIQ1575190.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="AC1Y77_001614"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="AC1Y77_001614"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIQ1575191.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="AC1Y77_001615"
     CDS             complement(9282..9869)
                     /locus_tag="AC1Y77_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIQ1575192.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="AC1Y77_001616"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="AC1Y77_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIQ1575193.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="AC1Y77_001617"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="AC1Y77_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIQ1575194.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="AC1Y77_001618"
     CDS             complement(12786..13721)
                     /locus_tag="AC1Y77_001618"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1575195.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4470 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DBNIBO010000068        13798 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.072, whole genome shotgun sequence.
ACCESSION   DBNIBO010000068 DBNIBO010000000
VERSION     DBNIBO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.072"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(24..1043)
                     /locus_tag="AC1Y77_001619"
     CDS             complement(24..1043)
                     /locus_tag="AC1Y77_001619"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIQ1575196.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="AC1Y77_001620"
     CDS             complement(1676..2680)
                     /locus_tag="AC1Y77_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1575197.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="AC1Y77_001621"
     CDS             complement(2693..3742)
                     /locus_tag="AC1Y77_001621"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1575198.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="AC1Y77_001622"
     CDS             complement(3759..4580)
                     /locus_tag="AC1Y77_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1575199.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="AC1Y77_001623"
     CDS             complement(4567..5346)
                     /locus_tag="AC1Y77_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1575200.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="AC1Y77_001624"
     CDS             complement(5363..5833)
                     /locus_tag="AC1Y77_001624"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1575201.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="AC1Y77_001625"
     CDS             complement(5845..6264)
                     /locus_tag="AC1Y77_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1575202.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9122)
                     /locus_tag="AC1Y77_001626"
     CDS             complement(6345..9122)
                     /locus_tag="AC1Y77_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1575203.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            9313..9537
                     /locus_tag="AC1Y77_001627"
     CDS             9313..9537
                     /locus_tag="AC1Y77_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575204.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10316..12031
                     /locus_tag="AC1Y77_001628"
     CDS             10316..12031
                     /locus_tag="AC1Y77_001628"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575205.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12034..13797
                     /locus_tag="AC1Y77_001629"
     CDS             12034..13797
                     /locus_tag="AC1Y77_001629"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575206.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4055 a         2826 c         2391 g         4526 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaataaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
    10141 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
    10201 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
    10261 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
    10321 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
    10381 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
    10441 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
    10501 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttccca
    10561 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
    10621 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
    10681 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
    10741 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
    10801 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
    10861 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
    10921 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
    10981 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
    11041 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
    11101 aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata tgaatcaaat
    11161 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
    11221 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
    11281 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
    11341 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
    11401 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
    11461 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
    11521 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
    11581 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
    11641 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
    11701 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
    11761 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
    11821 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
    11881 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
    11941 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
    12001 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
    12061 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
    12121 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
    12181 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
    12241 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
    12301 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
    12361 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
    12421 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
    12481 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
    12541 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
    12601 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
    12661 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
    12721 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
    12781 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
    12841 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
    12901 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
    12961 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
    13021 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
    13081 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
    13141 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
    13201 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
    13261 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
    13321 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
    13381 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
    13441 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
    13501 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
    13561 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
    13621 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
    13681 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
    13741 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaa
//
LOCUS       DBNIBO010000069        13739 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.073, whole genome shotgun sequence.
ACCESSION   DBNIBO010000069 DBNIBO010000000
VERSION     DBNIBO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.073"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(3..659)
                     /locus_tag="AC1Y77_001630"
     CDS             complement(3..659)
                     /locus_tag="AC1Y77_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIQ1575207.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            852..1130
                     /locus_tag="AC1Y77_001631"
     CDS             852..1130
                     /locus_tag="AC1Y77_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575208.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1149..2357
                     /locus_tag="AC1Y77_001632"
     CDS             1149..2357
                     /locus_tag="AC1Y77_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1575209.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2505..2870)
                     /locus_tag="AC1Y77_001633"
     CDS             complement(2505..2870)
                     /locus_tag="AC1Y77_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HIQ1575210.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3017..3790)
                     /locus_tag="AC1Y77_001634"
     CDS             complement(3017..3790)
                     /locus_tag="AC1Y77_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1575211.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3964..4824)
                     /locus_tag="AC1Y77_001635"
     CDS             complement(3964..4824)
                     /locus_tag="AC1Y77_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HIQ1575212.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4824..6242)
                     /locus_tag="AC1Y77_001636"
     CDS             complement(4824..6242)
                     /locus_tag="AC1Y77_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HIQ1575213.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6299..6748)
                     /locus_tag="AC1Y77_001637"
     CDS             complement(6299..6748)
                     /locus_tag="AC1Y77_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1575214.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7193..7456)
                     /locus_tag="AC1Y77_001638"
     CDS             complement(7193..7456)
                     /locus_tag="AC1Y77_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIQ1575215.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7555..8850)
                     /gene="rho"
                     /locus_tag="AC1Y77_001639"
     CDS             complement(7555..8850)
                     /gene="rho"
                     /locus_tag="AC1Y77_001639"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIQ1575216.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8847..10118)
                     /locus_tag="AC1Y77_001640"
     CDS             complement(8847..10118)
                     /locus_tag="AC1Y77_001640"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIQ1575217.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10283..11152)
                     /locus_tag="AC1Y77_001641"
     CDS             complement(10283..11152)
                     /locus_tag="AC1Y77_001641"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIQ1575218.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            11255..11467
                     /locus_tag="AC1Y77_001642"
     CDS             11255..11467
                     /locus_tag="AC1Y77_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575219.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(11444..13054)
                     /locus_tag="AC1Y77_001643"
     CDS             complement(11444..13054)
                     /locus_tag="AC1Y77_001643"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIQ1575220.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13276..13521)
                     /locus_tag="AC1Y77_001644"
     CDS             complement(13276..13521)
                     /locus_tag="AC1Y77_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575221.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
BASE COUNT         3908 a         2893 c         2343 g         4595 t
ORIGIN      
        1 catcaaaaga taatattgtc ttctaacaag tccatattca aaataacgat atgatggtct
       61 ttaacggtga taataccatc ttttttcagt tgttttaaaa ttctgtttac actgctagca
      121 gttgagatgc cacaaaattt ggcgatttct tcattcgtga tcgaaaaatc gatcagcatc
      181 ccttcttctg tccgcacacc gaataaatca tataattcgt aaagttgcgt acaaacagct
      241 cccagctttc cgtttaatag catttgctgc atttttttca tcgaataaag cagacgatag
      301 cggtaatact cttttacata gtgttgtagc tttgggtctt ggttgatatt ttgccaaaag
      361 cgcacacgat tgattcgata aaactctgca tattctgatt caactcgaat attatacggc
      421 gaatcgatga attgtgaata ttcgtctttt aaaagagaga cgatttcaag atctttgata
      481 tagcgaaggt taaattctct cccatcattt gaaataacac ttgcttttac cgttccctct
      541 ttcaagatat acgtatggga atcttccatc ccttcgaatg tcaaatattt atgatatcct
      601 ttgcgtataa tcggaaaatc atttttatcc aaatattcct ttaagactct actatccaat
      661 gatgctcccc catccaaact ttcatctaaa aagatcatac agtactttaa aactttttaa
      721 agtccaaagg tattttacta atgatattaa catttgttaa gtaccttttt ccatcactta
      781 tgcgattttt ttcctttttt ttattcgtta tactgcagaa gtaaaatttt ttaagaatag
      841 agggaaaaaa catgaattac tggatgaaca caatcattaa tcgattagaa acagcatatc
      901 aaacaagatt tgatatgaaa gcttctttag tctttttaaa tgatgcgtat caaaactcga
      961 tcgaattgat caaagcagtg gatgagaatc caacaaacga atgcgaagaa tttttaaatc
     1021 tgtttatgtc tacaagagat ttatttattc gccaacttgt tgatcgctat ccatccaatt
     1081 atcacgatgt agaagttcaa atacaaaaat taaaagcata ttcagcttaa ggggaatgta
     1141 taacatacat ggaaaagaaa ttatttaata aatattttat tggaatcaca ttgatcaatt
     1201 ttattgtcta cctgatttat tatctgttga tggtcatcat ggcagtcatt gcgcaagaca
     1261 ccttacacgc cagcttgagc caagcaggtt ttgcttctgg gatctatatc atcggaacat
     1321 tgctggcacg cttattcatg gggaaacagt tagaactatt tggtcggaag tttaccttaa
     1381 gaggcggcgc gatcttctat ctcctttcaa cattagcata tctttatacg ccaactatcg
     1441 gggcattgta tttagttcga ttcttgaatg gattcggcta cggtacagtt tctacagcta
     1501 caaatgctat cgttactgca tatatccctg catctaaaaa aggggaaggc atcaattatt
     1561 atggattaag tactagttta gctgccgctg ttgggccatt tttaggaatg attttgctga
     1621 atatgactga ttttcgtttc attatctggt tttcaattgt actggtcttc tttgttacga
     1681 ttgcctgcct tgtctttcct gtaaaaaata ttccattatc tgaagaacaa aaaatatctc
     1741 ttcgctcatg gaatatcaat agttttattg aaaagaaagc acttttcatt acaggaattg
     1801 cctttttgat gggtctttct tattcaagtg ttttatcatt tttatcttct tatacaaaag
     1861 taatccactt agttgctgct gggtcatttt tcttcgtcgt atatgctttg gtcatcacat
     1921 ttacacgtcc attgactgga aggattttcg atgtcaaagg agaacaatac gtgatgtatc
     1981 ctagctattt attcttaaca gcaggattat ttttactgag tgtaacgacg aatagtgtga
     2041 cattgcttat ctctggagca ttagtcggcc taggttatgg tacgtttatg tcaaatggac
     2101 aggctgtttg tttaaaaatc gtccgcgaac atcgaatcag tattgcttta tctacctact
     2161 ttattggttt agacctaggc ttaggcgttg gtccttattt gatggggatg ctaaaatctc
     2221 ttacttcatt tagaggattg tatgtgattg ccggagtcat tccattgata tgtacagcac
     2281 tctatcttgt taatggccga aaacatagtc atgaagcaga gggcgaattg attgaatcat
     2341 tgaaaaatga aatttgatct tgatgaatag ataaaaaaaa ccgttcagat ctgaaaataa
     2401 tctgagcggt tttttgagta aaaatagttt atgattattc ttaattaaac atctatttat
     2461 ttttaaagct ttcatttatc gataagaata gtttaactgt tgatttattc cattttccgt
     2521 tcatctggta cgatgccttg acttagcaac cacttgtgga agaaccattc tgccacaccg
     2581 atcaatagag caggaatcaa tactttcatc caaatatcga ctgtatcatt cataaaatat
     2641 agatagagcc acaagataac tgcagctgaa ccagcatctg cgatcgtcgc tgcgatgttc
     2701 cctactttac gtaagatcaa cagatcccct agcacataga tcatgactac aataattacc
     2761 ccaacaatcg ttgaatccat aaagctaaca ccgtataaac ctgtcagcac gacccacaga
     2821 atcgcccaga tcatgattgc cttcactacc aacgccttca catgtttcat tttctgcact
     2881 cctttctcct ggataaaaaa gtaagattat ctaacaagat aaccttttca ttattatacc
     2941 atcggctgag agaagcgagc aaagataagg tcctatctaa aaatgaggaa gccacttaga
     3001 acattcacgt aataatttat ccgtacagac attcgatatg gtgttccttt actttcgcat
     3061 actgttcttt tgtcagttct ttacttgtca ccagataatc gatttcatga aaagcaatgg
     3121 cttgatgcat gaatttatgt gcaatttttt gctgtgtaac taaaacggca agccggtctg
     3181 attgttttct aaaagctttt ttgaccattg cctctccttc atgagtttct gttactcctt
     3241 ggctgaaatc tagcccgcga caactcatta tcgctaaatc cgcataatat gacaatgcat
     3301 cgttataggc ttttgaaccg ttgattgttg ctcgtttcgg aacaattgcc cctcccaata
     3361 cagatacttt tgccgttgta tattcactca acatagcagc tgtgacgatg ccattcgtaa
     3421 tgatctgcaa accatcaaat cttgctaggt ttctgactaa gtgtaaagag gttgtactgg
     3481 aatccaagaa taagaccata tcatctcgaa tcaaagaaga agctgtatct gcaatcttac
     3541 gtttttctgt ttcttcttct aatttggtga tcttcatcag gtaaggcacg tcaatttttc
     3601 gattgtcaat aagcatggcg ccaccgcgaa ctctacggat caatccactt ttttccagaa
     3661 aaacaaggtc acgtttaatc gtggcttcgc tataatttac taactcgatc aaatcggtga
     3721 ctcttgcaaa ttggttgttt tttagataat tcacaatctc ctgctgtcta ttctccactt
     3781 cattcaacat aaaatcaccc catttacttt atatagtaac aggaaaaaag aggttgtgac
     3841 agaagtgatt ggcttcgaga aataagaaga aatttttcga aaaaactact tctgaacatc
     3901 gtgtgtggtt aagagggtat gacaagacta ttgtcatacc ctcttgacca cacattttta
     3961 tttttagtat aaactagcct tgcctactgt gttcaaaatt tccattttat gtttgaccac
     4021 ttcttgtacc tttttattag ctgctggata aattgcattc ggctcgtaca tcgatggatt
     4081 ttcactcaat atttctctca aagcattgaa aaagacgctt ttaagatctg agctcaaatt
     4141 cactttggca actccgtatt ttactgcttc gctgacttct gcgtccggat tacctgaacc
     4201 gccatgtaaa acaaatggaa gatcgatttg ttggttcaat tctttcagta agtcgatatt
     4261 taattttgga gttctgtttt tcggatacaa gccatgcgct gttccaattg ctacagcaag
     4321 tgtatcgatt cctgtttctt tcacaaaaat cttcgcttga tcaggatctg tataaatgat
     4381 tgtttcagca cctccttcag cagaaccatt attcccaatc gttccaagct cagcctccac
     4441 tgagacattt accgcatgag ctaacgcaac cacttttttc gtaatctgta cattgtcgtc
     4501 gaatgatttc aaagacgcat cgatcatcac ggaagaatag ccgtttcgaa tcgcccgtaa
     4561 gacatcttct atcgtaccgc catgatccaa gtggataacg atcggcagtt tacttgtata
     4621 agcataggct cttactgccg caacaaactc atcacttaaa taggcgattt catcaggatg
     4681 aatttctaag attactggtg cttctaatct ctctgcttct tcgatgattg ctttcatcat
     4741 atcatagctg caaatattga aggctggtac tgcaaattga ttctctttag ccactttcaa
     4801 taactctttc attgtatata acattacgct tccacctcat ttgttagatt ttcatttttc
     4861 gtccacttgt cttgtttttt ctctttcact gtttttttca atagtccaag cataaaggct
     4921 gtagtgactg tacctgatgc aattgccaag acatacatag gtaaattgtt cataacattt
     4981 ggaattagca tcacccaaat ccctccatga ggtgccattg aagtaactcc tactgccatg
     5041 gaaatccctg cagcgactgc tgaacctgtc atcaaactag gtatcacgcg gatcgggtca
     5101 gataccgcaa acggtatagc accttctgta atatacgaag cgcctaaaat ccagcatgat
     5161 tgacctgaag tccgttcttc tttggtaaat ttatgtttga ataataaagt ggctaaagca
     5221 atacctaggg gaggtgtcat tcctgcagcc atacaagcag caatcggtgc atttactcca
     5281 gctgacatca aaccgatcga gaaagtcgaa gtcgatttat tgatcggccc acccatatca
     5341 gctgccatca tgatcccgat aagtaatccg aacaatagcc cggatgtatt tcccaagcta
     5401 ttcagccaat ttgtcaaagc atccaacacc catgtgatcg gttgttcaat cacaaatacc
     5461 atcaaaagcc caataattaa gatcgataaa aaggggacga caatcagctg ataaatagca
     5521 gaaaattctt tttttacgct aattttattt gccagaaaat cacagatata ccctgctagc
     5581 acgccaccaa tcattccgcc aagaaaacct gaaccacctg tagaggccag taatccggca
     5641 acaattcctg gggcaaaacc tgcttttcca gcaatcgatg tagcaatccc cgcgcctaac
     5701 gcaggaacca tcatggcaaa cgctgtatct cctccgatcc ttgaaaatgc agctgctaac
     5761 acattatagt ctgctgagtt gggatcagcc gctgtaatcc caaaggcaaa actcagtgcg
     5821 atgatgattc cgcctgcgat cacaaatgga agcatgtaat tcacaccatt catgaaatga
     5881 ttatagattg tcggcttttt ctcatttgca gctggttctt tttcattaga cacaacgaat
     5941 acgccaatat ctctcaatgc ttcgtctatt actacttgcg gttctttgat tgctcggctt
     6001 gtagagacac gctttacttt tttcccggca aatcggtcca attcgatttc tttatctact
     6061 gcaagaatga ttgcatctgc ctctttgatt tcttgttcag ataactgatt ttctgttttt
     6121 acaccttgtg tttcgaattt tgcttgatgg tttagatttc ttgctgttac ttctaatttt
     6181 tctgccgaca tataagtatg agcaattcca gtcgggcaag cagttacacc aacgattttc
     6241 atgattattt tcctcctttt tcttcactat cgatactcgg acaaagactc tactattttc
     6301 attcttgact aatctgtctg atgatatctg ctggcgtagt ggcttctttt attccctttc
     6361 taaaatctgc attcatcaat cgtctggaga gtgtgctgag aatctctaga tgcatatctg
     6421 ctgatttttc aggaactacg atggcaaaaa tatattcaat tggcatttca tctatcccgc
     6481 tatagtccgt taatttggct gtatgaaaaa aacacagtgc tggttttgtc acatatttgc
     6541 tttttgcatg gggaatccca aatccatctt ctattcctgt ggagatctcc tcctctcgtt
     6601 tgaatagtgc cttaacgact ttttgaacat ttcttacctt atttgttttt ttcaacacat
     6661 cccctattcg ctcaaataat gatccttttg aatctatcga tggatcgaaa acaatcagtt
     6721 cttctgcgat caattgatca aacctcataa aaaacctctc ccccacaaaa aatgttaacg
     6781 ttttcttgga actgaggtca gtatagcatc gctagctgta ccgttcaata ttatgaatga
     6841 acagaggaaa aagacccatc atccgcgtgt ataagtcatt aaaatgaacg atctagctca
     6901 caaacaatcg caagtctata acaaaacaaa aagccttcct tgaaaggaaa gcttagcttc
     6961 taattttagg taaatggttt tttacttcga aataacccct cttcatttcg ttaatataag
     7021 gtgtttttat caagtggata attaaaacta tatactagtt tttcattgtt ttgtgcatta
     7081 tgatcctacg actttttcag catcaaacga agttttaaat tggcaaaaaa cataaaaaaa
     7141 cagactatcc ctatcagata gtctgctaaa cgttgagtta tcagcgtttt tattattccg
     7201 cagcgtttgc gtctttgaga ccgtattttt tgttgaaacg gtccacacgt ccgtctgctt
     7261 gtgtgaattt ttgacgtcca gtgtagaatg gatgtgagtc agaagttact tcgacacgga
     7321 tgactgggta tgtattaccg tcttcccatt caactgtttc ttgtgaagtt tttgttgaac
     7381 ctgacaagaa tttgaaacca gttgttgaat ccatgaatac tactgggtgg taatctggat
     7441 ggatgttttc tttcatgatt ttttcgctcc tttgccctgc tccatttgct gaaccagagt
     7501 tgatttgtcg atttacaaca ttaacatctt aacatgtttc agtcaaggtt gcaattatct
     7561 tttcagattt ctggtcggat tttttttacc aaaggagaca tcttgaaatt ctttgaaaaa
     7621 ttgctgattg ttttttgttt tacgcaaaaa gcggatcagt tgttccgtat attctaatga
     7681 atctccagtc atattttttc gaagtttcca aatttcttct aattcttcac ttggcatcaa
     7741 taattcttct tttcgggtac ctgatttttt gatatcgatg gctgggaaga tccgacgttc
     7801 agataattca cgtgaaagtt gtaattctag atttccagtt cctttgaatt cttcatagat
     7861 cacgtcatcc atacggcttc ctgtatcgac taaagcggtc gctaaaatcg tcaaactgcc
     7921 accttcttcg atgtttcttg ctgccccgaa aaatttctta ggtttataca atgcggctgg
     7981 gtcaatcccg ccactcaatg tacgtccgct tggtggaacg actaagttat aggcccgagc
     8041 aagtctagta atactatcca tcaagataac gacatctcgt ttatcttcga ctaaacgcat
     8101 tgcgcgttcc aatactaatt cagaaactcg agtatggttt tgcggctgca gatcaaatgt
     8161 agaagatacc acgtcgcctt tcacgcttcg ttctagatcc gttacttctt ccggtcgttc
     8221 atcaatcagt agaacgatta attctacttc cggatagttt tcggtgatac catttgcgat
     8281 ttcttttaat acagaagttt tccctgcttt tggcggtgcg acgatcaatc cacgctgacc
     8341 aaaaccgatt ggagaaaaga tatcgatcat tcgggtagat agtctttgcg gtgttgtttc
     8401 cagttgaagc tgtctttgcg gataaagagg tgttaaggct ggaaaatgcg gacgttcttt
     8461 tgcttcttcc ggatcttttc cattaacgct ttccacgtgc atcaaaccat aataacgttc
     8521 cgattcttta ggtggacggg cttttcctgc cactttgtcc ccatttctca aactgaatcg
     8581 tctgatttgt gaggcagaaa tatagatatc ttctgcactt ggtccataat tgattggccg
     8641 taaaaaaccg taaccatctt gaccaacgat atccaatatt ccttccatat aataaaatcc
     8701 ttgtttctct gcttgtgcac gaatcacagc tagagataat tcctttttat tcatcttact
     8761 gtaataagga attttaaatt ctttggcata actataaact tcttttaacg ttttgttctc
     8821 tagttctgcc attgtcaaat aatcgctcat tcagctccca cttcctctat catttcaatt
     8881 gatgccccta aggctgttag tttctcgata atatgatcat aaccgcgtaa gatataatct
     8941 acattgtaaa tcgtcgttgt cccttctgcc aatagtccag caatcactaa acaagcacct
     9001 gcccgaagat cagaagccac gacttctgtg ccgtgaagtt tatttggacc attcacgatg
     9061 atcatatttc cttccacggt aatatcagcg cccattcgtg ctagttccgg cacatggttc
     9121 acacgtttcg catagatcgt atcgatgatt tctgaagtgc ctgtagtcat caatagtaac
     9181 gcagtaagtg gctgctgcag atctgtggca aatcctggat aaggataggt catgatgttt
     9241 gcgggtttta attctttcga ggggtaaact tcgatttcat cttcgctgat tttcatatgg
     9301 acaccaattt cctgcaattt tgctataaag ctttccaaat gttcaaagat cacatttttc
     9361 actttgatac catttcctac tgcagctgct aatgctagat aggttcctgc ttcgatgcgg
     9421 tcaggaatca tgaaatgccg acagccatgt aatgtttcga ctccttcgat acggatcaca
     9481 tctgtacccg ctcctcgaac ttttgccccc atattattca gtaaagttgc cacatctatg
     9541 atttccggtt ctcgtgcagc gttttcaatt actgtctgtc cttttgcttt tacagcagct
     9601 aacatcacat tgatcgttgc tccgactgaa accacgtcca tgaaaatccg attgccacgc
     9661 aatcctttat tttctgtccg taaatacatg gcaccatgtt catttgtcac ttcagcacct
     9721 agagcttcaa atcctttgac gtgtaggtca atcggtctag ggcctaaata acagccacct
     9781 ggcaggccta ctaccgcttc gccaaattta ccaagcagtg ttcccataaa ataataagaa
     9841 gcacgcaaac tattgatttt tccgtgcggc atcggaatcg atacgacttc tcgaggatcg
     9901 atttctagta tgttgttgga aaaatggaca gttgcgccca tgatctctaa aatttcaatt
     9961 aatgaatgaa catcttgtat gtccggcaca ccttccaaga tcactgggga atcagctaag
    10021 attgcagctg gaatcaatgc gactgcacta ttttttgcac cgctgatcgt tacttcacca
    10081 gctaaaggtc ggtttccttt gattacgatt tttttcatct gtttccacct tgtttattaa
    10141 tcttttttca tggatatgct tgtattttat catagataaa aaaaagaaac gagcatggtc
    10201 tgccgtttta agtaaaaaag aatggagagc gacttcaaaa gtcgttctcc attcctatat
    10261 atcgtatctg ttatgtggat gcttacgctt tgttagctga accgaaccag tcgatatgag
    10321 atttagcatc ttcaacgatt gctgctttac ctggagctaa taatttacga gggtcgaatc
    10381 ctttaccttc tttatcttta ccagcttcga tatatttacg agtagctgca gcaaatgata
    10441 attggaattc agtgttaaca tttacttttg agatacccat tgagatagct ttttcgattt
    10501 gatcttgagg aatacctgat ccgccatgta atactaaagg catgtctgga cctacagcat
    10561 cagcaattgc ttgtaagtgg tcaaaagcta atcctttcca gttttctgga tattgaccat
    10621 ggatgttacc gataccgcaa gctaagtagt cgatacctgt agcaaccatt tgtttgcatt
    10681 cttcaatatc agctaattcg ccgttaccga tgattccgtc ttcttctccg ccgattgaac
    10741 caacttcaca ttcaactgag ataccttttg catgtgcttt ttcaacaacg tctttagcta
    10801 atttcaagtt ttcttcaaat ggtagatgtg aaccatcgaa cattactgaa gtatagccta
    10861 attcgataca ttccaaagca gcttcgtaat caccatggtc taagtggata gcaactggaa
    10921 cagtgatgtt cattgcttca actaaatcag tgattaaatc tttacagatt ttgtaaccac
    10981 ccatgtattt agctgcaccc attgatgttt ggataagtac tggtgcttgt tttgcttcag
    11041 ccgcttcaag gattgcttga gtccattcca agttgtttgt gttgaatccg ccgacagcgt
    11101 aaccgccttt acgagcagct tttaaaaatt cagctcctga tactactggc ataataaaat
    11161 tcctcctaag ttttgtaaaa ctaaatttgt taagacaatt cttaaccaac gaccctattt
    11221 taccaaacat ttcggtagtt ttctactaaa atcaatgaaa atgaaaattt tcaagaagtt
    11281 ttttgcaaaa aataaaatat ccaatagcgc tttaatcgaa aaaagtcttt tgctttctat
    11341 cttttttcct acaaataata acaaatcaga aaaagagaaa agtgacaaga aaattgctta
    11401 tccccttctc ttgctacttt tctctttcta tcaagttcaa aaattatttt gtttcgtgat
    11461 ctgctaatga tgctccgaca aatgctttga ttaatttttg cgggcgattt ggacgagaaa
    11521 tcaattctgg gtggaattgg catcccacaa agaatttttt ctctggcagc tctacgattt
    11581 ctactaaacg gttatctggt gagacacctg aaaagactag cccttgttct tcaaacaatt
    11641 gacgatattt gttattgaac tcatagcggt gacggtgacg ttcttgcaca acatcggctc
    11701 catcatatgc ttcagccgtc ttcgttcctt ttttgatttt acatgggtat aatccaaggc
    11761 gtaaagttcc acccaaattc tcgatgtttt cctgatcagc cattaaatca ataatattgt
    11821 ttgctgtatc tggattcgtt tcagctgatg acgcatcttc taatccaacg acgttacgtg
    11881 caaattcgac acaagccatc tgcataccaa gacaaatacc taaaaacgga acgtcatttt
    11941 cgcgagcata acgaatcgct tcgattttcc cttctactcc gcgatcacca aatccaccag
    12001 gaaccaagat tccatctgcg tctttcaaac gttcttctac attttctctc gttaattctt
    12061 gtgctttgat ccaatcgatc tcgatgtctg aattataggc gaaaccagaa tgtttcaatg
    12121 cttcaactac tgagatatat gcatctggta actcgacata tttaccgaca agcgcaatcc
    12181 gtactttttt cttcaagttc aagacacgtt cttctaattc tttccattct gtcatgtctg
    12241 cttctggtgt atctagtttt aaatggtcgc aaacaatttg atccattcct tgtgcttgta
    12301 aaactaatgg gatggagtaa agtgtatcta catctggcga ctcgataact gcttctggtt
    12361 cgacatcaca aaattgtgcc aatttgtttt tcacgctttg agaaacaggt tgctctgtac
    12421 gtaccaccaa aatattcggt tggataccta aaccacgtag ctcttttaca ctgtgctgcg
    12481 ttggtttagt tttcatttct ccagcagctt tcaagtaagg aatcaatgtt gtatggatat
    12541 acataacatt atctgtaccg acatctgctt tcatttgacg aagtgcttca aggaatggca
    12601 aagattctat atcccctact gttcccccta cttctgtaat gatcacatct gaatcagtca
    12661 ttttagctgc tcgcatgatt ttttctttga tctcatttgt aatatgagga ataacttgaa
    12721 cagttgctcc caagtattcg cccttacgtt ctttacgcaa aacttcagaa tagatttttc
    12781 ctgtcgtcac gttggagtat ttgttcaaat tgatgtcaat aaaacgttcg tagtggccca
    12841 gatccaaatc tgtttcagca ccatcatctg ttacgaatac ttctccgtgc tgatatgggc
    12901 tcatcgtccc cggatccacg ttgatgtatg gatcaaattt ttggatagtt acttttaagc
    12961 cgcggttttt caataagcgt cctaaagaag ctgcaacaat cccttttccg attgatgaaa
    13021 cgacaccgcc ggtcacaaaa atatattttg tcataagttc aaaaactcct ttttaattta
    13081 tgtgcaggaa aagtctcaga caataaacaa agctccctat tcttcaataa gaatagggag
    13141 ccgaaatttc tgtaaacttc tgaccctcta aaagggtgcc caaggtgtat aatacaagct
    13201 gactaaggga aagtcaagta ctcaaatgaa tactatccgc ttacaatttt ttctttttcg
    13261 atatcctttg agaaattatt tcttaagtcc tttatgagtg caaaaaaaat ttaacccaca
    13321 taataagctt gatataaaca tagctaacgt taaacctaag caaatcagaa aatcgttact
    13381 gaaaaatttt aagtgaaata atagataaaa aaataaaaca aacattatga tagataatat
    13441 cccacctaac aaagaactac tttttcctac ttgcagcaac agtttatata gcttatcgta
    13501 gaaacaatct ttttctttca cccttattta cctcctttac gtaaaaaacg tagtccacag
    13561 ttctcggatt tttggcatat cttttctatt aaataatatc aaaaacaaaa caattccact
    13621 aattttttca aagatgacct ttttttcaac aaaagtgcct tgtagggatt cattgaaggt
    13681 aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa gtcgtgcctc cttatttct
//
LOCUS       DBNIBO010000070        13605 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.074, whole genome shotgun sequence.
ACCESSION   DBNIBO010000070 DBNIBO010000000
VERSION     DBNIBO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.074"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..902)
                     /locus_tag="AC1Y77_001645"
     CDS             complement(<1..902)
                     /locus_tag="AC1Y77_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIQ1575222.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="AC1Y77_001646"
     CDS             1185..2153
                     /locus_tag="AC1Y77_001646"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1575223.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="AC1Y77_001647"
     CDS             2266..2544
                     /locus_tag="AC1Y77_001647"
                     /EC_number="2.3.-.-"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575224.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="AC1Y77_001648"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="AC1Y77_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIQ1575225.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="AC1Y77_001649"
     CDS             3001..3756
                     /locus_tag="AC1Y77_001649"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1575226.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="AC1Y77_001650"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="AC1Y77_001650"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIQ1575227.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="AC1Y77_001651"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="AC1Y77_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIQ1575228.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="AC1Y77_001652"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="AC1Y77_001652"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIQ1575229.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="AC1Y77_001653"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="AC1Y77_001653"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIQ1575230.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="AC1Y77_001654"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="AC1Y77_001654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIQ1575231.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="AC1Y77_001655"
     CDS             8493..9035
                     /locus_tag="AC1Y77_001655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIQ1575232.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /gene="atpA"
                     /locus_tag="AC1Y77_001656"
     CDS             9058..10614
                     /gene="atpA"
                     /locus_tag="AC1Y77_001656"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIQ1575233.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="AC1Y77_001657"
     CDS             10628..11530
                     /locus_tag="AC1Y77_001657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIQ1575234.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="AC1Y77_001658"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="AC1Y77_001658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIQ1575235.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="AC1Y77_001659"
     CDS             12973..13395
                     /locus_tag="AC1Y77_001659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIQ1575236.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4502 a         2436 c         2965 g         3702 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaa
//
LOCUS       DBNIBO010000071        13539 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.075, whole genome shotgun sequence.
ACCESSION   DBNIBO010000071 DBNIBO010000000
VERSION     DBNIBO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.075"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(74..1030)
                     /locus_tag="AC1Y77_001660"
     CDS             complement(74..1030)
                     /locus_tag="AC1Y77_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HIQ1575237.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="AC1Y77_001661"
     CDS             1134..1736
                     /locus_tag="AC1Y77_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIQ1575238.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="AC1Y77_001662"
     CDS             complement(1824..3323)
                     /locus_tag="AC1Y77_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIQ1575239.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="AC1Y77_001663"
     CDS             complement(3339..3863)
                     /locus_tag="AC1Y77_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIQ1575240.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="AC1Y77_001664"
     CDS             4161..4910
                     /locus_tag="AC1Y77_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIQ1575241.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="AC1Y77_001665"
     CDS             4901..5686
                     /locus_tag="AC1Y77_001665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1575242.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="AC1Y77_001666"
     CDS             5655..6371
                     /locus_tag="AC1Y77_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1575243.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="AC1Y77_001667"
     CDS             complement(6473..7492)
                     /locus_tag="AC1Y77_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIQ1575244.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8512)
                     /locus_tag="AC1Y77_001668"
     CDS             complement(7511..8512)
                     /locus_tag="AC1Y77_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIQ1575245.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="AC1Y77_001669"
     CDS             complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="AC1Y77_001669"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIQ1575246.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10014..10850)
                     /locus_tag="AC1Y77_001670"
     CDS             complement(10014..10850)
                     /locus_tag="AC1Y77_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575247.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11034..12251)
                     /locus_tag="AC1Y77_001671"
     CDS             complement(11034..12251)
                     /locus_tag="AC1Y77_001671"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIQ1575248.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(12400..12579)
                     /locus_tag="AC1Y77_001672"
     CDS             complement(12400..12579)
                     /locus_tag="AC1Y77_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575249.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            12634..12717
                     /locus_tag="AC1Y77_001673"
     tRNA            12634..12717
                     /locus_tag="AC1Y77_001673"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12668..12670,aa:Leu,seq:gag)
     gene            complement(12957..>13539)
                     /locus_tag="AC1Y77_001674"
     CDS             complement(12957..>13539)
                     /locus_tag="AC1Y77_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575250.1"
                     /translation="DMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGN
                     TMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVG
                     IYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVK
                     ELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4090 a         2767 c         2329 g         4353 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
     8641 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
     8701 aacttatgta aagtgatttt tactagaaca caaaaaaaac cttaaaacca aacttaagaa
     8761 gtttagtttt aaggttaact taaattatat tatgcgtgga aacgttccac gccatctaga
     8821 taagttgctt ccaatgtcat atcagggttc aatacgataa agtctgcatc gcgtccttga
     8881 gcgatttttc cacatgtatc atcgatttta cagctgattg ctggaatcag actagccatc
     8941 atgactgctt gttctggtgt tgctaatccc cagtctacaa catttttaat tgcttctttc
     9001 aatttcaata tacttccagc taagttgcca gattctagac gtgctgttcc ttcttttact
     9061 actactggaa attcgcctaa attgtaattt ccatcaggca tcccaccggc catcatacag
     9121 tctgtaatta atgcgacatg atcatgtcct gctttttcca ttaggattcc cgcagcattt
     9181 ggatgtacat gatggccatc acaaatcaat tcagaaaaga catgttctaa agataataac
     9241 gcacccacca tacctggttc gcgatgattc aatccacgca taccattgaa agcatggaca
     9301 aatacacttg cgcctgcttc aacagctttt tgcgcttctt ctagtgtagc attactgtgt
     9361 cctagagaaa caactactcc ttcttcagtc acttgtttga caaactcttc tacaccttga
     9421 cgttccggtg ctaaagcaat ttttttgatt aaaccacctg aagcttcttg ccattcatgg
     9481 aaagtatcca aatcaggatc cccgaaataa cttgggtttt gtgccccttt gtgttcttct
     9541 gtgaagaaag gaccttcaaa atagattcct ttgattttag ctcctggtgc ttcctgatac
     9601 attttgccga ttgtctcagc tacatctttt aaacgttctt tgcttgacgt caatgttgtt
     9661 ggtaaaaatg aagtcactcc acaagataac agaccttcag acatcacttt gatgccttct
     9721 gcatcattat ccattacatc atgattcata tagccgtgaa tatgtgtatc taccaaacca
     9781 ggagcaatcc atttgccgct ttggtcaatg atgttgactt ctccctcagg tagttctttg
     9841 gtgtattcac caaaaagtcc atcggtaatt tcaagatagc ctgcatgttt gacacctgac
     9901 gctaaaaaga atttgtcagc atagataaaa gttctcattc tattctctcc ctttgttttt
     9961 ctacctctaa agtactctta tttagagaag aagtcaagaa tggtctacac catttacaaa
    10021 ttttttgaaa tttgtttcac agcttgtgct aagccttcat agaatgctgc tctttctggt
    10081 tcttctttac gcttgtatat taagctttta agcacaaata aatcattgag gtaatagtag
    10141 ctattcttct cttgagccca atagattcct tgattcacct gttctaacgc ttcgtcgatt
    10201 tcattttgat ggaataagaa atttccataa ttataaaaaa tttgcgaaaa ttcacttttt
    10261 gatcgttcat cagctgattg atgcagcagt tggatgcttc gactcaaata gtagcgtgct
    10321 tcagccgttt ttcctgccac accatatatt tttccgatac aactaatcag ttgtatttct
    10381 gtgtcagaaa catggattct ttctttttgg ctagagtagc ttaatgcttc tttcaaataa
    10441 taaagggact gagagatatt ttgacgcaac gtgaattcgc aaacaccgag ataataataa
    10501 tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa tatcagaaga ttttagtact
    10561 tgttctaact tccgataatc acgtttttgg aaacaactat ccaacagttc aaataattta
    10621 tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat gatcaatcgt tacccctagc
    10681 cgttgacaaa tctgcatcat caccaataca ttcggcagtt cctcattatt ttcgatccga
    10741 cttaatacac tttgtgaaca aatatcatgc gaaagcattt tttgcgtcat tttttgttct
    10801 tttcgaatct ctttaatgat cgttccaaag gactcgatta acgtttccat aagtatcctc
    10861 cccaaaatat gcgtattcgc attctattcg tattttactt taggaagtcg caaacttcaa
    10921 gaaaagactt gcaactaaat aatatacttt agttatttaa gaaaactcca ctagaaagag
    10981 agcctcagtg tgtttgtctg tggccctcct actgaaaaat ttggttttta aaatcatttt
    11041 tggcgatagg ttgctaggaa ttctctcatt ttcttagctg tttgtttcaa atcatccatt
    11101 ttaggaagat aaacgattcg aaaatgatct ggctggttcc aattaaatcc accaccatgt
    11161 acaagtagaa tatgatgttc atgtaaaaag tccaaaacga atttttcatc atccaaaata
    11221 ttgaatcgct ttgtatcaat ttttgggaat atataaaaag cagcttttgg ttttacggct
    11281 gaaagaccag gaatatcatt gattgcatta tagatatatt ctctttgttc atagatcctt
    11341 ccgcctggca ggagcagctc atcgacactt tgataaccgc ctagcgcggt ttggataatt
    11401 tgctgagaca atacattgga acacaaacgc atggaggaca acatattcag cccttctata
    11461 tagcctttga cgtgtgactt atctccactt aggaccatcc agcctactcg aaatcctgct
    11521 acacgatgag atttggatag accgttcagc gtaacaacga acaaatcagg cgctaatgta
    11581 gcaatcggaa tatgttctag accatccatc actaagcgat catagatttc gtcggaataa
    11641 ataatcaggt tattttgtcg agctacttca acgatttctt ctaataattc ttttggataa
    11701 agtgccccag taggattatt aggattaatg ataacaatag cttttgtccg actcgttatt
    11761 tttgatttga tatcatcaat atcaggatac cattctgcct cttcatcgca aatataatga
    11821 acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca acggatagtc cggcattgga
    11881 accagtactt catctccatt gtccagcaac ccctgcatac acatcgtgat caattcgctc
    11941 actccgttgc cggtgtagat atcattgatc gtcacattcg gaaaatgttt cagttggcaa
    12001 tattgctcaa ttgcttttct agcagaaaag atccccttgg aatctgagta gccttctgag
    12061 tcacgggcat tcatgatcaa atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt
    12121 gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct cttcttgcat ccgctccgct
    12181 tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt ctaatttgtt ggatttttca
    12241 aattctctca tcacttagcc aatccctcat ttcaaaaagt tagaactttc caaatttttt
    12301 gttatttact atttcaatat catactccat cctagaaaat tttgccactt atttaggaaa
    12361 aacgtaagta ttaaaaaaac acaaacaagc gttgagaact taattgtcat gatagtcatt
    12421 ccatagattc gctgcccaat ccattgcaat tccacaaatg attcctagca taaaaccaag
    12481 caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag atcattccca gtcctgaaaa
    12541 ttctccgtca tatttctcca tcttaataag tcctcccatt tttgagcttg acaactatgt
    12601 aaaacctcgc tatcattagt gtgttattat tacgcagatg tgttggaatt ggcagacaag
    12661 catgattgag ggtcatgtga gcgttagctc gtgtgggttc gagtcccatc atctgcacta
    12721 caactcgtga agttaatttt gcttcacgag tttttttgtt tgaacgtttc tttatgccat
    12781 tatatacata tcactgtatc ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg
    12841 tgagtatatg aaggtcggaa acaagctact cgtagaaact tgcaagtgtt aaaacaatta
    12901 atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg agggattttt tgtatattat
    12961 ctgtttatca ccgttattat aaaggtacaa ataccaagca cggttaatcc aaattgcata
    13021 aattcatttt tttgtacact catgcttttc attagctctt taactgccat tagtactaag
    13081 atggcgataa caacataaag tatatagtac gtccgcattc cggaagccca tactgtggga
    13141 gaaaatccca taattaacct tgaacaaaat cccattatca tcaaaataac taaaaaaata
    13201 gaagttttct tattgtcaaa acttaaataa attccaacaa ttaaacatag taataatgcc
    13261 aagactacta aagtagctat ccaagtacct ggataaaaaa gtgacagttt cgttcctaac
    13321 tctgtaaaga gattattcag gttactacta ctccaaatca ttgccctttt atttccattg
    13381 acgtaagtaa agctttgacc cattgtatta ccaaaataaa taatcaggtt taagaaaaac
    13441 ggtaatgcag tcaatatacg cacataatag ttttgacatt tcctatatgt tagaaggaaa
    13501 attaagaaaa atagcaataa aaataagata ttcatgtct
//
LOCUS       DBNIBO010000072        13358 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.076, whole genome shotgun sequence.
ACCESSION   DBNIBO010000072 DBNIBO010000000
VERSION     DBNIBO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.076"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(18..722)
                     /locus_tag="AC1Y77_001675"
                     /pseudo
     CDS             complement(18..722)
                     /locus_tag="AC1Y77_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="AC1Y77_001676"
                     /pseudo
     CDS             795..971
                     /locus_tag="AC1Y77_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1147..1716)
                     /locus_tag="AC1Y77_001677"
     CDS             complement(1147..1716)
                     /locus_tag="AC1Y77_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIQ1575251.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="AC1Y77_001678"
     CDS             complement(1790..3079)
                     /locus_tag="AC1Y77_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575252.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="AC1Y77_001679"
     CDS             3406..4281
                     /locus_tag="AC1Y77_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1575253.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="AC1Y77_001680"
     CDS             4295..4618
                     /locus_tag="AC1Y77_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575254.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="AC1Y77_001681"
     CDS             4615..5367
                     /locus_tag="AC1Y77_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIQ1575255.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="AC1Y77_001682"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="AC1Y77_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="AC1Y77_001683"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="AC1Y77_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HIQ1575256.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="AC1Y77_001684"
     CDS             complement(9831..12017)
                     /locus_tag="AC1Y77_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1575257.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="AC1Y77_001685"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="AC1Y77_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HIQ1575258.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="AC1Y77_001686"
     CDS             complement(12239..12679)
                     /locus_tag="AC1Y77_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HIQ1575259.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="AC1Y77_001687"
     CDS             complement(12753..13292)
                     /locus_tag="AC1Y77_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HIQ1575260.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3741 a         2805 c         2380 g         4432 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag
      541 atgttttgtg ttacccataa atgcgatttg attcccggtt ggttataagg agtgtccaac
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaa
//
LOCUS       DBNIBO010000073        13118 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.077, whole genome shotgun sequence.
ACCESSION   DBNIBO010000073 DBNIBO010000000
VERSION     DBNIBO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.077"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(64..975)
                     /locus_tag="AC1Y77_001688"
     CDS             complement(64..975)
                     /locus_tag="AC1Y77_001688"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIQ1575261.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1378..1956)
                     /locus_tag="AC1Y77_001689"
     CDS             complement(1378..1956)
                     /locus_tag="AC1Y77_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1575262.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(1976..2644)
                     /locus_tag="AC1Y77_001690"
     CDS             complement(1976..2644)
                     /locus_tag="AC1Y77_001690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575263.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2659..3729)
                     /locus_tag="AC1Y77_001691"
     CDS             complement(2659..3729)
                     /locus_tag="AC1Y77_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575264.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3900..5264)
                     /locus_tag="AC1Y77_001692"
     CDS             complement(3900..5264)
                     /locus_tag="AC1Y77_001692"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1575265.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5261..6820)
                     /locus_tag="AC1Y77_001693"
     CDS             complement(5261..6820)
                     /locus_tag="AC1Y77_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575266.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6876..9356)
                     /locus_tag="AC1Y77_001694"
     CDS             complement(6876..9356)
                     /locus_tag="AC1Y77_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575267.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(9340..9675)
                     /locus_tag="AC1Y77_001695"
     CDS             complement(9340..9675)
                     /locus_tag="AC1Y77_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575268.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9689..10723)
                     /locus_tag="AC1Y77_001696"
     CDS             complement(9689..10723)
                     /locus_tag="AC1Y77_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HIQ1575269.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(10813..11493)
                     /locus_tag="AC1Y77_001697"
     CDS             complement(10813..11493)
                     /locus_tag="AC1Y77_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HIQ1575270.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(11777..12880)
                     /locus_tag="AC1Y77_001698"
     CDS             complement(11777..12880)
                     /locus_tag="AC1Y77_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIQ1575271.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         3765 a         2700 c         2249 g         4404 t
ORIGIN      
        1 cgataataag cccggccaaa cgaaaaatat gaggagctat ttttgtttgg ctgggcttat
       61 ttattactga aaataagtgg tcaatgcttg tgtcaaactt gcagcaatct gctgctgata
      121 actgtctgtt actaattcct ttaaatcact atcattattc atataaccca tctcgattaa
      181 aatagaaggt tgaagatttt ctcgaagaac ttgatagttc ccagtggaga ttcctcttga
      241 atccagaggc agatcagtga aggagtcagc aattgtctgt gctaatgttt cctcttgtcc
      301 atagtaataa tatgtcgtga ttccagttgc ttcattcgca tattccgtag agtctgcgtg
      361 taaactgata aagacatctg cctttttttc ttcactgtag tcgcatatat ctccaagctg
      421 tactaattca tctgtagtat gggtcaaata aaccgttgct ccagcatcct ctagcgcctt
      481 ttttactttt atggctgtac ttaatgttat atctttttct tctgtttgtt catcattgct
      541 taatgcccca gtatcatttc cgccatgccc aggatctaat acaatcactg catcagatag
      601 ctcttccggt tttgtttgta tattctttgt gactttggtt ccatcttcga gggctaaata
      661 tccagtatag ccatcatttg tcttgatctg ataagcattt tttgtttcag aaagaaactc
      721 cgccagctct ccgccgttta tagttgcaag tggggtagtt agcgagtcat ctgtatatag
      781 tgtacggtca ttttttccga ttttgattaa tgtctcttgt tctgtatcat caaatgattc
      841 ctttgtcttt gtaaccgtac tgttcgtact gctgaccgtt tgtccaacta tctttttttg
      901 accaaaaaca tctatttgaa aacagataat aatgataatg attgcaaata caaaagctcc
      961 aattattttt ttcattcttg actctcacct cgtagatcca gactaagggt caaaacttaa
     1021 aatcagctta agtacgcaaa aaaggggctg taaaagatat aaccgtagaa aataacatta
     1081 ctgctaaaat gatttttttc atttttggca tatcctgaaa tctccttttg actattcgtg
     1141 gtgattcatc tacccaataa atagtttcct tcattctaca aacaattaat ttcttcgtta
     1201 ttaaattaaa acacttcctg gaacaccgac ataataaata aaaatatacc ctgataatca
     1261 tgagagaata acaaatttgt atttatgaaa aactacctat ataaaaaaaa caagatataa
     1321 cgatcttcgt catatcttgc ttcttcacct aactattttt ctccctatat aacagcttta
     1381 cgcttctcct aatacaccat tcaaatataa atcgatcaat tgcgccacat ttagcggttc
     1441 ttccacaggt cttttcgacc gagtgatgta aggtgctaac actaagaaaa aatgccttgc
     1501 tgcgatttct tttgaaatcc catttcttag cacagattgg tttgctgaaa agatttcttc
     1561 aaaaggttca cgataatttt tcttccatag aaagaaaaga tgatctcttg tttcctttga
     1621 aagattgctt tcaagatcgt gaagcatcag aaacaaatcg accagatgtg tctcaatcaa
     1681 aaactgcgcc atttttgtca aggttacttg aaatcccgtt tcgcttgttt gaacgatttc
     1741 tctcaaagct tttccaacat cttttaagtg gttttctaaa actgccgtat atagtttttc
     1801 tttatcaccg aaataatgat agagattggg ttgggtgatc ccaacttttt gtgcgatctc
     1861 tcttgttgaa gtgccgagat atcccttgga cataaacaag tcagtcgctt ctttcaaaat
     1921 tttttgctgt gtatctttgt tgtatcgatg gaccattttt ccacctgctt tttggttatt
     1981 ctttttcttt caatacacca tcttccatca taaatattct atcgcatttg tcaatcagcc
     2041 gtgtatcgtg agtaaccatg atcgttgctt tatttttgtc ttttgattct tttgccaaga
     2101 tatcgactac ttctcgtgcc ttttctgaat ccaaactagc agttggttca tctgctagaa
     2161 taatggacgg atcattgtac aatgcacgag cgattgcgac cctttgccgt tctccaccag
     2221 atagttcttc tggatacttg ttgatcaact tatccacgcc aagctgctca aataattctt
     2281 ttgcttcttt ttgacgatgt tcatgtttga tcttatctac taataataac tgctttttta
     2341 ctgtcagaaa aggaaccaga ttggaagctt gcaaaataaa gccaatttct ttaaaacgta
     2401 gtttaacgcg ttcactttct ttttgattac tgaattcttg atcattgata aatacagagc
     2461 cactgctagg cgtctgcaat ccgcctgcca aagtcaaaaa agtactttta cctgaaccag
     2521 aaggaccgat gattgcaaca aattcccctt tatcgatact catggttgtt tctttcaatg
     2581 ctttaactac ttggtcgcca tcttggaaac ttttttctac ttctttaaat tctattgctt
     2641 tcatgctctt cctccttctt atccaattgc cttcaacgga tcgattttta cgattgtccg
     2701 aacagaaaat agtgcgccta acacagctac taccagcatc aacaagctaa tgacgccaaa
     2761 aaacagccaa ttgctttgga atggaaccgc atcaggtaga acaagggatg ttcccactgt
     2821 tcctaataga ccaagaagaa taccaacaaa tgataaaatg aatgtttgtg ccactacaga
     2881 acgcgcaata aatcctcctg tgatcccttg tgctttcatt acaccaaaga tattaatctt
     2941 ttgcatcgtc agtacataaa tgaaaatccc gatcacaata gccgcaataa cgatcaaaaa
     3001 tccgatcata aatccaaaag tcaatacttg cgcattatat ccaggtaatt cattgatgaa
     3061 tttagaaatt ttgatctgtt ctaaatcttc aggtaaatta ctgattttac ctctgacaac
     3121 tattgcgtta atccgtgcat tttcacttgt atcttctttt tcgaagcgga tctcttggta
     3181 agcattgatg gttgtataga gaactggtga gacattgaat ttggcatgat ccgtaaagcc
     3241 agttagttta aacgtcttgt cactaccaga taacttaact gtatctccta cggctagacc
     3301 atattcttct ttcaaactaa tatcgcctac tgcctcatca tcgttatcaa aagcttttcc
     3361 ttcaacaaga ttgggcatga tgaattgatt tttatcgata ccgaagaagc taacattgat
     3421 ttttccttct tctgtactgt ctttactagt cacgactcca ggtgtctggg caagatacgc
     3481 cactttccca ccttcgactt cttctgctat cttcttagta atcatggaca tccctagatt
     3541 tgaatttgca tctttcgaaa gcacaatcga atctgcctcc cacttgtcga ctgctgttcg
     3601 attatcttga gctaagccat aagctaaccc agtcaaaaaa aataccaggt aggcgatcaa
     3661 aaacatcacc cctgcaacta acgcataacg taactttgaa tgcataattt catttaatgc
     3721 taaaaacatt taactcctcc tcctgaaata atcattttat acaacaattt tatcacttga
     3781 taaatttttg gcgaatatta tgctcaaaaa attcttccgc tatttcatta tatgaatttt
     3841 ttattctcga ttgattaata tttattatat ccccacacaa taaaagaccg tgatatttct
     3901 cacagtcttt tatctactct attgctattt gctctcttag ctagtacttt aaaagctaac
     3961 tcattgaggt agtgaaaacg agaggtgtgt tcagacagaa atccaaaatc ctcaactgct
     4021 agctgtggaa tcgtagtttt gtgtttcacg atatccgcaa gcaggttcaa atctgataat
     4081 aagaattctt ggcttatcag ctgtccgcca attatttcta atgtatccgg atggtagatc
     4141 agctttgcat ttactgtttt gttttcttca tcgtattgtg gaaatagatc aaattctcct
     4201 gaacacgaac cggtattttg ttccaagaat ggcgcttcat cttctgtcag ccctacagtt
     4261 cctaaaaagt agtctataat gactgctgct gttatctttt gtctgaggtc gtaagcggtt
     4321 ggctgaccac tcaaagttag cgctactgct cttgccatat gtacagctcg agtaactaaa
     4381 ggtaaaaaga gatgccgttt atttttgtca aaactgactg ggacgatatc tccaatcgca
     4441 tagatgtccg gatcacttgt ctgcagatat tcattgacta tcacagtctc atcctcatac
     4501 aaagccactt tttcttttaa caatgtcgta ttaggacgag tttgggcaga aaaaatgacc
     4561 atatctgcct ttattgcttg agatagaagt tgtacggata ctactttctc ttctgatttt
     4621 ttccaatcga tcaagaattc atttggataa aaattgattt gcttagtttg cagctgattt
     4681 tgaaaactgt tgatcatatc cttatcgaaa tatcttgata aaacattcgg catataatca
     4741 actagatgaa taatgtatcc tttatttctc aaagcatctg ctaattccaa tccaatataa
     4801 ccagcaccga agatcactac ttcttttgct tcttttaaag cggcaagtac ttgcttagat
     4861 tgcactagat ttttataggt gtagacattt tcagcttcaa tttgtttata caaggtgaca
     4921 ggagaggaac ctgttgctac gattaattta tcataggtta tggtctcgtt ttttccttct
     4981 ttggatgcga ggatgagctg atgtttgtct ggatcaagtt cagttactct ccattcggtg
     5041 atcagttttg ccccactttc ttgaagagtc cgcatgttat gggcaacttc agctaaacta
     5101 cgtatctttc cttttaaata aaggttgatt ccatttgaaa catagcctag tccatcttta
     5161 tgatcttcat cgatcagtaa aacttctgct ttcggttgaa gttttcttag atttagggca
     5221 gctgtgatgc ctgcatggga agcaccaatg atcacgattt tcatgtttta ttcatcctta
     5281 aattcaattt ttctaaataa cattctttta aaaattggtt caaatagggg aaatcataat
     5341 tatattcgtt gctagtgaat acaaaaactt tagcggactt ctgtgacggg tactctttca
     5401 attggctagt caaaatgata tcatactgct tttctttctc gtattcgact aaatcatatt
     5461 tgatcgtatt cagcaattgt tcctggatga tttctttcac gatatccgcc attagccttt
     5521 cataaggaag atcataagca atttgcagac gtaattctaa ctgcttgatc agcgtccgca
     5581 aaatagatgc atagcgagca aataattcaa agcgggcatt ttcctcgtct acttcagtaa
     5641 aatcaacaat agcaaatgtt tgtctgacca atctttcaca taaaaccagg aattgtttat
     5701 cttctacttc agttaaccgc tgttttaatc cttcttggcc aaaaatattc atccatcctc
     5761 taaaataggc aaaatgagcg tgattttgca tcactgtcaa cgcaatcttt tgcgaaatct
     5821 cttctggaaa aacataaaaa gagaaatatt cttctatcga ttgtttaaac gttcgattca
     5881 attgagctac tacgtcgtct ttgtattttt cgcttatcaa aaactcctgg acatgcgagt
     5941 tatgtgtatc taatgtaaag ttactgatca tgaaaagata aaaaaccaac gtttcatttt
     6001 gattccaagg aaaggcatat tgaaagaagt aacgggtcaa aaatgtatgg aggtccaata
     6061 aaaattgtgt cgtttgaaac aatccaacag gaagattgct ataatctggt ttggattttt
     6121 tcgtacagcg cattcgcttc ttcataatgg aaagccatat ttcaaatttc aatcgtcctt
     6181 cttctgtaat agtaaaacta aactcttttt gcagcagatc taaaaaagtg tttcctgata
     6241 aatcactgta ttcttttttg ttttcaagat aaggacgtcc aaaccaaaat aattggaaat
     6301 aaaaataaca aatttgactt tctggaccgg tcagccgccc gctttttatt ttcagatgaa
     6361 actcatttaa agaatcattt aattccttca ctctgcgaga caaagtagcc gaactgatat
     6421 taagttccat gacaagtttc tgccgatcaa atgagccatt ttccaacaga taaagaagta
     6481 tctgatattt tgtagaatag cgcaagtagc aatgataata aacatcgact gctgctatat
     6541 ttccatgtct gattaataaa atattccctt cttggataac aatttccaat tgtttgatat
     6601 tttccaagtt agcttgtaaa taaaacagat cttctttcaa ggtagcccga tcaactgata
     6661 agcgggtaag tacgttatta acggaaatct ctcctttttc ctctaataaa agttggaaaa
     6721 gtttgatttg ccgttgttca tttttttcaa gcaaactgaa aagttccaag tccactcctc
     6781 caagcatttc tgattttaac agcagaagtt tccttctcat ccttgaaaaa gaggatctgt
     6841 gacagttatc acagatccat acaaattcta atcacttagc tggttggccc agctgtcagt
     6901 tcccatgtga ccatgccttg atacgttgcg ccaggttgag cattcgtact gctatttacc
     6961 tctaagagta aaccatcggt atcgggaaag tcaacgagaa cctctccttt agctgttcca
     7021 tcactctcat aaatcaacgt gttttgacct tgctgaatca attgttttga tccagaatga
     7081 ttgaagtata attttgttcc gcttaattct tgaccttgat cattcttaaa taaagaagtc
     7141 aatgtccctg tcagttgcca aggagacgtg cttggaagcc gagtatcccg cacatcaaaa
     7201 gaaaaatcgc cttttgcttc ataataactt gtcttcaacg gtttggggag atcgttacca
     7261 aatgtaaaat catctggtac attaactaat gttaattctt gtttggggac aagaattgtt
     7321 ttagcagatg tttggacaac tgggttagga agcttagcac catctgctaa tgtaatattg
     7381 ctggaaagta cagctgcctt tgtcagtatg ctgacggatt gtacagaagt atctacagta
     7441 tttaattgat agttgtaagt aagatgaatg gtatctccta ttttgctcaa tagatctgct
     7501 gggacagaaa ctccttgttg aatgccggta tctgcgatag ttgtaccatt taattttaag
     7561 gaaccattga tataagtagt attttcagga agtgagatcg tgattttttt gtcggttatc
     7621 gcatccccag attttgttaa cgtgaatgtc acattttctt gaatcgtatc tcctgataga
     7681 gcagacaggg gatctcctgt ctcaggttgt ctgctaaggt tttttagctg actatctgca
     7741 ctcacagatg gaaaattagc cactgttatt tgaggaagag tggtgattcc agcttgttca
     7801 gactgccatt tgtacgcaaa agacggatac atgttagtaa gtacatcgtc caccaagcga
     7861 taatcaaaat aatattgccc tgaagccaat gcaggtagtg tagaagtagc tgtatttaca
     7921 cccccgttat ttgattggat cgtttggaaa agttgatctg tccatcctgt cgctaatttt
     7981 tgggttcctt tcgaataaag cctgtaatgg atcgaaccct tttttagttc agttgctgac
     8041 caattggccg tgacttggta cgatgcagaa gaaaggagct ttccccactg actatcggct
     8101 gttaagtttg ttggtaaaac cgttccgtct agcttgtact gctccaatac ctttaaagca
     8161 acaggagaaa gcaatccgcc ttgcgcatct ttcccactgt acacgatatt ggctgtctga
     8221 ttcaatccac ttgtgcttgc tggagtatga gtcagtattg ctccttgtgt aacgtttttt
     8281 cctgtaaaat agttgactac gcgatcaaag cgactaaatg gcaactgatc aaaacgtcct
     8341 tcagtcagcc ccatcgtcaa tgtccgacta ttggtagagc ctggaaggtt ctggttattt
     8401 ccatccacgt attctgtggt atcagcaaat gtattatatt ttcctgttga atcacttgtt
     8461 gagatgtaac tattccacgt atttatctct gcacttgcag aaaatgcata tgctcctgga
     8521 ttcaagtttc cgccagttaa tttaggataa aatctacctg cagctggagc taaaatcgaa
     8581 ttgaacatac gatcttggat aatattattc gaatcataaa aatcaacaga aaaaacggcc
     8641 tttttagaca tagaaggttg taatttaccc ttggtcaaaa ttttgtttgg actcgtatca
     8701 tatttatctc cgttttctgt catcttcgcg acattagctg taacatcaaa ttgaagcggg
     8761 atatcgtcca cgttgaattc gcctcgcgta ataatagcaa taatatctgc caatagagta
     8821 tttgtatccc acgaccccat cccaaaacgc aaggtatggc ttcctgctga taataaacta
     8881 ccgctagtaa ccttgatggg ggcaccggca atatacaagt aggcgttgtt ccagtcaatt
     8941 gcttttaata catcactgct tgatacatgt tccggcaaat tgatgatcgc cacaaatgca
     9001 aatccttgtg cagtgatatt caccaaatca agtacatttg ttagtttcac atggctttcc
     9061 caatgaaaac ggaccgtgcc acctttccaa ggatcatttg aaccatcgtt caaataagta
     9121 gcagtcaatt taggcaattg gttagcccca gttagttgga caagcttccc gccattgact
     9181 gcactacgtc caccatttgg aggtacgata ttttcatacg tgtatggatc ataagaaatg
     9241 gcaggaggcg ttgctccaac agcttctgcc gaaggtgaaa agataaatat acttaaaaca
     9301 aaaagcgaag taaaaaagaa ggagcgaaaa aatgaaaatt tattttgtag caacatgttc
     9361 atcctccttt tcctttcttc tccataacaa caaccacgca agaagccata attctatacc
     9421 aatgatcaaa taaagaaaac ggatcttttc ttcattcgtc tccgggaata cgccatgttg
     9481 attagattct ttataaatcg gtagctgtgt ctgctgtttt gatgcgccat tgactgaacc
     9541 acttggatgc ggaactggac ttagatcata aaatgtaatc gtacctgaac ttttggtatc
     9601 tgcttggact ggctcttgcc agctgaagaa cagcaaactt gcaataatcc ctcccaataa
     9661 gatcgatgtt ttcaacgaaa tcacctcatc atgttgtttc tccttttttc tttttcttgt
     9721 ggacccacaa aaacagcaaa atcaaccata acactagaac tgcagcaacc aatcctgcat
     9781 aaagccatgg gaaagattgt tcttgtactg caaaaacgga attttcattt actttttgtg
     9841 ctttttcttt tttgatcgta aagttttgtg taaatcgcca ttcattactg tcgcctgatt
     9901 ttgctatgat ttctgcgcgg taatcgccgg cttttgcttt atcaccagct aaatcaagat
     9961 tcaagtgaaa attcgaattt ggtgcaacta aaaattcatt agacgtattt tcaatataaa
    10021 gctgattttc ataaaagatc ttgctcgtaa atatcaaatt gctgataatc ctaggctgag
    10081 gattttggaa acgaagctga acagttggct ttgcgttgac ctcgtccaaa tctcctgata
    10141 aaagtttcag atccggttct atcgtctctt gactttcttg taaaacgact gcgagattat
    10201 aagcaatacg atttttgatt tgtgctcctt ctgtagcatt ttctgtttca gcttcttctt
    10261 taggtgaaac atgaataccc gctaatacac gtcccttaaa gctttttgct gggattttca
    10321 cttctatagg tatagttgtt tctgcatgtg ctgatacatt gatttccttt ttcgcaatga
    10381 gcaaaacgtc acggacatca aacggcaatg tctcatctaa cgttgggttt ttttgactat
    10441 aatcgatcgt tccaccatca ctcgttaccg ccggattgac agagacctca aatgttcgtt
    10501 cttcatctga agaattgctg ataattactt ctagtgtctg attttgttcc ggtgccacta
    10561 ataaatcaaa ataaccgatt gctttatcta tttggttctc tggaaatacc ggtttgattc
    10621 caaagtctcc tgctgaatcc tttgcttggc tgattgacgg aaaaatgaaa aatagactta
    10681 aaatagaaaa aaacgaaaca aacagtcgtt tactttgatt cattgtccac cacatccttt
    10741 caacaacaac tgcggtaaaa aatccttcac ctatagtatt ttcaccgcag tttgttgaat
    10801 ttatttcata ctctatgcta ctgggccagc cacgaggttc caagtcaaat cagccgtgaa
    10861 tgttccagct gctgctttcg gcactttcag tgtgatattt gctggatcat aaacagaaag
    10921 ccatgttccc agaccttgat cttttgctgc tgtaaatatt ggtgtcgctg cacttgcttt
    10981 actcaaccca gttacagttt gactagttgg tccagtggat tcgttatctg acgtactttt
    11041 aacagtggat gttccaaaat ctaattcagc gccttgcaaa acttgtgaac cgtttacaaa
    11101 atctgaaacc gaaactgtca cgttccaccc ttgtgcttgg ccatctgcac ctacacctcg
    11161 acgatctgat acttggagat aaggtgtatc gttttttgaa gcgtctactt gatacgtttt
    11221 tgttccgctt tcaatctcgt gtgtaccaaa tggcaattca ggaactacat ccaatgttaa
    11281 agaacctgtc tggccagttg ctggattttc cggatctgct ggctctactg gttttgaagg
    11341 atcagtaggg ttaagaggct caacggtatt tgttggttca gtaaaggtaa tatctccttt
    11401 tgaagtcgct tgaccctcag caaaagctgg tgttgccaag ccgcctagaa gaagtgttga
    11461 aagtaataat gtcccaatca tgttcttttt cataataaat cactcctttt tcttagtgac
    11521 ctaagtatag tagagacagt atgcaaaaaa tcctcatgaa ttcatccgct tacttgaaat
    11581 tatgaccctt tttgtataaa agagaaaaaa acaataaaat cgtttacatg aatattcttc
    11641 cttttttatt aagtatgaaa aaatatttca attttatttt ttcgtttcaa attattctat
    11701 tagtgacatc cttgaactat aaaaatagga gtacggcatc atctgccgca ctcctatttt
    11761 tttcgtcatt tatttcttaa ataaaacgat tctcacgcaa ttttattttt ttgatatctt
    11821 ctactgtttg tgttccagct aattgcatga ccatttccaa ttctgtttta aagaaatcaa
    11881 acacttgatg gactcctgta cttcctccta aagacaatcc ataaataacg ggtctgccaa
    11941 tagcaacaag atcggcacct gatgcgattg ctttgaaaac atgttgtcca cgtcgtacac
    12001 cgctgtcaaa cacgattggt acacgtttat ctactgcttc tgccacatat tgcaacgaat
    12061 caaacgcagc aggcccccca tctaattgac ggccaccatg gttagatacc caaattccac
    12121 cagcaccaga ttctaatgat cgatagacat cttcttctga ttgaacacct ttcacgtaga
    12181 ctggcaaatc agaatgagca gcgataaatt ctacatcttt tggactaagt ttttgtttgg
    12241 aagatttata tacagcatcc atcgtttggc ctactcctga ttgataagcc tgaacgatcg
    12301 gcattggcag cgggaaggta aacccgtttc tgcggtctgt ttcacggttt ccgccaactg
    12361 ttgcatccgc tgtcaatacg attgcttttg ctccattacg cttggccact tcaagaatat
    12421 ccaaattgat tccattatct ttgctcatat aaaattgaaa ccattgcggt gctttctccc
    12481 cgcctgcttc tcttatctct tctaaagtac aagaagcgta agaacttgct gtatagatcg
    12541 tcccaaaatc tgctacacct tttgcagaag ctttctccgc cttcacatga gctaatccat
    12601 gagcagcaac tggtgccatg atgattggtg cagtcagcat ttcttcgtca aaatgtgtag
    12661 ttgtatcggg taattctaca tctcttaata catgaggaat aatcaaacga tgattgaatg
    12721 cccgttcatt ttcttgataa gtaaataagt cgccagcacc actgctgatg taaccataac
    12781 caccagctgg aatcacttgt gaagccgcga tttctaaatc ttccatattg ataaaatcta
    12841 ctattccttc atttgtacct gcttgatatg ttttttccat ctcaatgcct tctctctata
    12901 aaatttgaat gatgaacaat atgatagcgt ttacactttt tatattactc ttcgacattg
    12961 atacaggcaa ataatctgtt tacatttcag ccattccaga tgtcactact ctggaagaaa
    13021 tgattgtaat aaaaatagat aaaaaaccga tgggactttg acaaaagtag tgtcaaagtc
    13081 cctttttttg aataaacggt gttcaaaagc cattttct
//
LOCUS       DBNIBO010000074        13052 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.078, whole genome shotgun sequence.
ACCESSION   DBNIBO010000074 DBNIBO010000000
VERSION     DBNIBO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.078"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(45..2165)
                     /locus_tag="AC1Y77_001699"
     CDS             complement(45..2165)
                     /locus_tag="AC1Y77_001699"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIQ1575272.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2395..3573
                     /locus_tag="AC1Y77_001700"
     CDS             2395..3573
                     /locus_tag="AC1Y77_001700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIQ1575273.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3589..4347
                     /gene="aroD"
                     /locus_tag="AC1Y77_001701"
     CDS             3589..4347
                     /gene="aroD"
                     /locus_tag="AC1Y77_001701"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIQ1575274.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4370..4855
                     /gene="ybaK"
                     /locus_tag="AC1Y77_001702"
     CDS             4370..4855
                     /gene="ybaK"
                     /locus_tag="AC1Y77_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIQ1575275.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4926..6179)
                     /locus_tag="AC1Y77_001703"
     CDS             complement(4926..6179)
                     /locus_tag="AC1Y77_001703"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1575276.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6295..7644
                     /gene="gdhA"
                     /locus_tag="AC1Y77_001704"
     CDS             6295..7644
                     /gene="gdhA"
                     /locus_tag="AC1Y77_001704"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIQ1575277.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7756..9102
                     /locus_tag="AC1Y77_001705"
     CDS             7756..9102
                     /locus_tag="AC1Y77_001705"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIQ1575278.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9410..9796)
                     /locus_tag="AC1Y77_001706"
     CDS             complement(9410..9796)
                     /locus_tag="AC1Y77_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1575279.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10064..10213)
                     /locus_tag="AC1Y77_001707"
     CDS             complement(10064..10213)
                     /locus_tag="AC1Y77_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575280.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10675..10887
                     /locus_tag="AC1Y77_001708"
     CDS             10675..10887
                     /locus_tag="AC1Y77_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575281.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11165..12964)
                     /locus_tag="AC1Y77_001709"
     CDS             complement(11165..12964)
                     /locus_tag="AC1Y77_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HIQ1575282.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4282 a         2347 c         2575 g         3848 t
ORIGIN      
        1 acaaagagcc taattttgtc gctgctcctt ttaaatttat tttatcattc ggaagaactt
       61 gtggtcgttc cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac agttgattgt
      121 cccttgtgtt cactaaagac actagttgac ttgtcaccaa gagatttttc agcttctttt
      181 aatttctcta agccattctt gtagtcataa tcactggtat caattgggtc taatccacta
      241 ttcgtataga accgaagaag atcaccatta ttgatttggt cagaaacagc taactgttta
      301 tcaacttttt ctttcagtga atcaatttca ttctgtactt cctcagtagg ttcagtaata
      361 gcttcaccag tttcgttact atagatcgta ccaccgtagt aagtatattt tggtgtaaca
      421 aaatcaccat cacggaaaga aacaagctgt tcatgatctt tcgagaatag atcttgtcct
      481 aactgaatat aattttttgt atctacacct agtaagtgaa gtaatgtagg caaggcatca
      541 acttgtccgc cgtaagtgtg attgattccg ccctgtgttt gtccagggat atggatcata
      601 taagggacac gctgcatttg tgcattgtcg tagtttgtcc aagtagaact tgtttttcca
      661 actaattcag ctagattttt atttcttgaa ttcgagacac cgtaatggtc accgtaaagg
      721 acgatgactg aattatcata caagccgctt tctttcaaat aattgaagaa ttcttgtacg
      781 gcggtatcta aataattggc tgtcgcaaag taaccgttga tcgtttcatc atttgtattg
      841 gctaacggga atcctgcttc gtcattggtg aattttgaat aaggataatg gttagataca
      901 gcaatgaatt ttgaataaaa tggttgttgc agacgttcca agtattgtac ggattgttgg
      961 aaaaatggtt tatcatgcaa cccgtattgg aacgaattgt catcattaac atcataataa
     1021 gaagaatcaa agaaataatc atatcctaaa ttcttgtatg tctcgtttcg attccagaat
     1081 gtaccagcgt tcccatggaa taccgcacta gtgtaacctt gtgtttgact taaaatatca
     1141 ggtgcagctt ggaaagtatt ttttccgcct aattgtgtga acaaggagcc ttgattcaag
     1201 ccaaacaatg aattttccat cagcgtttcc gcatcgcttg tttttcctgc ttttacttga
     1261 tggaaaaagt tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt gatgaaaggt
     1321 gtaacttcaa gctggagacc attctcatct gtcaatttat aatcgatcag gaattgctgg
     1381 aagctttcta aatggatata aatcacattt tttcctttag cgagcccgaa caaatcatca
     1441 tttggtgctg cataatgttc ttttacataa ttttctactt ctttcatatc attgggactt
     1501 gcttctgccc gcacttggtt tgtctggtaa gtttgaacac cgtcataaac catgaaagca
     1561 ttcaagccaa gatatttgac caaataatca cgagagaatg ttcgagtcag caattctgga
     1621 cgatcagctt ccgccataaa aagatttcct gagaaaatca taactgctaa tgtagagatc
     1681 gcaaaagcca ttcttgctcg gatcgggcga gggtccaact tgattttttt tgcaatcagc
     1741 agaacagtca agataataaa atcgatccaa tagatcacat catatgggcg aaacagtcga
     1801 atggcacttt ctcccagacc actcgctact ttcccagcac ctaaaatcgt gttgatcgta
     1861 atgaagtccg tgaattctcg ataataagtg acattcgaaa aaacaagcaa tgtcaaaaga
     1921 aaataaatga ccatcatcgt gatataagca actctagggc gtctgatata aagagcaatt
     1981 ccaagtaata acaatgtagt tgcaataggg ttgataaaca agataaagta ttgaatgcca
     2041 ctttcgattc cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa ccaaagtaaa
     2101 acaactagta aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt ttttatacat
     2161 ttcaagataa tcttcctttc tgcacttctt aatcacagaa gtgtcatgaa accatgttac
     2221 acagctcact tattctcatt tattttacta gtatgacaat atagtgtcaa ttgaaaccag
     2281 aagtttatgc tgtttcatta aggttttctt taagcttcat gaaaaaatga gcagtaagac
     2341 aagtcctatg ttttttgtta tacttgggaa gagaaagcta aagcaggtga aggaatgaaa
     2401 gtaaaagtaa aaaaaagagc aagcaatcga ttgaaaaaag gatatccact gatccaaaaa
     2461 gaagaccttg ctcatcccca aaaagaaacc acgcaatggg tggaattagt tgattcgcaa
     2521 aaccaattta tcgcaaaagg atatctagga aagcagaata agggaatcgg ttgggtattg
     2581 agccagcaga acatcccatt tgatcaatct ttttttgaag aattttttgt gaagtcaaaa
     2641 gaaaaaagaa ctttcttttt ttcagatgat tctactaccg cttttcgtat attcaatgga
     2701 gaaggagacg ggatcggcgg tctgattgtt gattattatg caggttatgc tgttttttct
     2761 tggtataacg aaacgttata tgcacatcgt gaattattga ttcaagcttt tcgtgaaatc
     2821 tacccggaaa tcgtaggagg ctatgagaaa attcgatttg aatcagtaaa tctgcctgag
     2881 agccagttca tttacggaaa agaggcaccg gaacccttga tcgtaaaaga aaacggagta
     2941 aaatttgccg tttatttaaa tgaaggatta atgactggga tctttttaga ccaaaaagaa
     3001 gtacggggat cattagcaga aggtttcgct tctgggaaaa cagttttgaa catgtttagt
     3061 tataccggcg ccttttctgt tgctgccgca atgggagggg caatcgaaac aacaagtgtc
     3121 gatctagcaa aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa tggtttggat
     3181 gtcgatcagc aaaaaatcat tgttatggac gtattcgagt atttcaaata cgccaaaaga
     3241 aaacaatttt tatatgacat gattgtattg gatccaccaa gttttgccag aaataagaaa
     3301 aaagtctttc gcgtagcaaa agattatggg gaactggtca aggatagtct ggatatttta
     3361 gcagaagacg ggatacttat tgcgtctaca aatgctgcga atgtctcgat agaacaattc
     3421 caaacaatga ttgaagagga attccatgcc aaaaatgttt cttttgaaca acttgcttta
     3481 tatcgtctgc caaaagattt ccagacgacg gatacctttt tagaaggcaa ttatctgaaa
     3541 gtgtttgttt atcaaattaa aaataaccat tagaaggagc gatgcagaat ggtttgggta
     3601 aagggattga atataggaag cggtagacca aagatttgtg cgcctcttat cggggccgtc
     3661 cgagaggaag tattacgtga agcagttctt gcacgtgaag caggggccga tttagtcgaa
     3721 tggcgagtcg atcattatcg tgaagttttt tcttttgatg aagtagcaga aacattggtt
     3781 cttatccatg agatgcttga tggactgccg ttattattta cgtttcgtac cttagctgaa
     3841 ggcggagaac aagacatatc gatccgtcag tatcgcgacc tttatgaaca tgtgatacac
     3901 acaggattag tagatatggt cgatatcgag ttgttcaaag tagaaagtct aggaaaaaga
     3961 ctgatagagg aaatcaagtc tttaaacatt cctttaatta tcagtagtca tgattttaaa
     4021 gaaacaccag cagaccctgt cttgctttac cgattgaatg ttatggaaca ttttggagcc
     4081 gatatcggaa aactggcagt aactcccaat aatgaacgag atgttttgcg gttgatggag
     4141 ttgactagac gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc catgggaaat
     4201 cttggtaaaa tttctcgtct ggctggcgga acgacgggtt ccgttatgac atttggttcc
     4261 ttaagcagcg attctacttc tgctccagga caattaccgg tcaaagcatt gaagcaaatt
     4321 attcaattat taaatgaacc gttgtagaac aagtttgagg tgatattttt tggcaaagaa
     4381 aaaaatagta aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc cctatacaga
     4441 gcacgaatat gagtgggacg aaagccacct aagtgcgtca tcggtcgctg aacagcttcc
     4501 agaaagccaa tctcatatat ttaaaacact ggttgctgtg ggcaatgtga cggggcctct
     4561 tgttgctgtg ataccgggag aagccgaatt gaatctaaaa aaattagcaa aagtttcagg
     4621 gaataaaaaa gtagaaatgc tccatttaaa agatttggaa gcaacgaccg gatacatacg
     4681 tggtggctgc tcgcctatag gtatgaaaaa actatttcct acttatttag atcaaattgc
     4741 agaaagctat gaacaaatca ttgtctcggc tggcagaaga gggctacaga tggagttagc
     4801 acctcaagat atctgtgcat taacgagcgg acaatttgca gatataaaac aataaatcat
     4861 acgtataaaa gaaagaagga ggccgggatg ttgtttgtcc agtctccttc tttctttttt
     4921 atacattatg ctctttgccc ggcattcatt cctgctacat gccctgtagc gaaagccgct
     4981 gtgatattaa agccgccagt ataaccattg acatccagta attctcccgc aaaatacaga
     5041 ccatcaatga ttttactctc caatgtttta ggataaattt cctttagaga aacccctccg
     5101 ccagttacaa atgatttctc aataggaaaa gttttgttga ttgatagctt aaactctttg
     5161 catagttgaa caaaatcttg aatttgtttt tgagaggctt gttgtcctgt cagatgatcc
     5221 atttcaagcc gctcaagaaa gaaaactaac aatcgctcag gcaatatacc atgccaagca
     5281 ttgaccaagt tctttttggt tgctttactt ttttctgtca atatatgaat aagctcttgt
     5341 ttggtttgtg tagggaaaca atccagagaa agtgtcactg gttctcccgt tttctctaat
     5401 tctttattga caaaacttga acaacgcagt gcagccggac cagaaatacc gaaatgggtg
     5461 aaaagtaaat ccatcgtatg ttcagtcaat acacgacctt ttttatttaa aacgcgtaaa
     5521 gtgatgtctt gtaaagaaag cccttgaagt gttttctcct gaatatacgg ttcatccgat
     5581 atcaaggggg actctgtcgc ataaagaggc gtcaccgtgt gacccactcg cttcacgatc
     5641 ttgtacccat ctccagtaga accagtagat ggataagtcc gtccaccagt tgttaagata
     5701 atggcaggag catggaactc ttcgaattcc gtgcgtatac cgtaaatttt atgttctttg
     5761 tggatcaatt tctctactcg agtagagaat agaagcgtca catctagttc tttcagacga
     5821 ttgagcaatg cttcgataat cgtttttgat ttatttgtta caggaaacat ccgtccatga
     5881 tcctcttctt ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc gaaattattc
     5941 cattgcgcaa acgtactata taaaaacttt ccatttccag gaatatgtgc aatcaaatca
     6001 tcaacaggac gattgttcgt aacattgcac cgaccaccac ctgtcatcag tagttttttt
     6061 cctgcttttt tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc tgcactgatc
     6121 gctgccatca tcccgcttgt tccagcgccg actacgatca catcatacag tttttccatt
     6181 ccctcacctc aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa atgttttgag
     6241 ccaaaagaga aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca
     6301 aacgcaacag aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa
     6361 gaagaatttt tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact
     6421 catcccgaat atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt
     6481 caatttcgag tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt
     6541 atccaataca actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc
     6601 aatctaagta tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt
     6661 ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat
     6721 gagatcatgc gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg
     6781 attgatgttc ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc
     6841 gaatataaac gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt
     6901 tggggaagta agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat
     6961 ctattgaacg aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg
     7021 aatgtagcca tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt
     7081 tcagattcta gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa
     7141 gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt
     7201 tatcatgctg gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt
     7261 gctactcaaa atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa
     7321 atcgtagcag aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa
     7381 gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct
     7441 ttagaaatga gtcagaacgc ccagcgattg acttgggaaa gagagcaagt ggatgagcag
     7501 ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc
     7561 gacgaaaaaa atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca
     7621 atggcagcag aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt
     7681 gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta
     7741 taaggaggag aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc
     7801 aacgaacaag agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg
     7861 gaaggtactg gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac
     7921 aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta
     7981 ttagttgtta tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg
     8041 aatcacacat tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt
     8101 gccggaaatt caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc
     8161 gacttttcag taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc
     8221 cgtgtattca aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc
     8281 tacgctacaa cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg
     8341 gaaactttcg tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt
     8401 ttgttaccaa tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat
     8461 gcaagcaaag catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca
     8521 atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca
     8581 ggaatgcaat acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac
     8641 caaaaaggca tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa
     8701 ttcatccaag aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt
     8761 aaatcaatca caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga
     8821 aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac
     8881 ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat
     8941 acaatgtact tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca
     9001 ttcgaccaac caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaaccg
     9061 ggctttgaag atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta
     9121 gatttaaata atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta
     9181 ggtactcaaa catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca
     9241 cagtcccttg tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact
     9301 ttaagaacag tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt
     9361 aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc
     9421 ctaatttttc ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga
     9481 tccgcttttc gcttgctttc aactgcaaga tcgttccttc ggtgtaagca gaaccattgg
     9541 cagagtcacc agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat
     9601 aagcatctac gttgtattta aaacctcttc caattacttc cccattatct gctttgatat
     9661 cagtttctgc aggcattgga gctttgacta ccgcataata atctttacct acataagtag
     9721 actgatagta gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc
     9781 ctaaaatttt tttcatgaaa gatctccttt tttattttta ttatattaaa tattatacag
     9841 ataataacgg aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt
     9901 tgatattaga acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata
     9961 tatgtaatga atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga
    10021 ttttccgaga tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta
    10081 atgctcggac tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat
    10141 caatcgccat aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt
    10201 ttgtaaacgt cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt
    10261 taaactttcg aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca
    10321 tttatcattt tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat
    10381 aagtagccca atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc
    10441 tctttataaa taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa
    10501 caaacgtttt tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa
    10561 agatactcaa aataaaaaat aaaaaaatga ataaaaacgt tttcaaatac aaggtaattt
    10621 gttattataa aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta
    10681 aaagcagaag taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc
    10741 ggctgtattg aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa
    10801 cgagactatc aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt
    10861 agcgcaccta ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt
    10921 ataatataaa taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga
    10981 aaatgggaat tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg
    11041 cgatgaagga atcaagagaa agttaagcac acattatgag cgtcaaacaa aaaacgcata
    11101 tacctgcggt ggcaggttat gcgttttttg ttgtataaag acttttttca ttaacgattt
    11161 ttgattagcc gactcccaaa acaaaattta gcagtttatc tgaataagtg atttcttcat
    11221 ctgtttgttt cacactttca tttagtagtc ccatctcatc ggttatgata ccatccacac
    11281 cataaaacat catgcgactc atggcatctg aatcgttgat tgtccataca tatacttctt
    11341 ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat cgtactatat tccattgtca
    11401 gaaaatcagc aggcgtcaca gggggcccaa caatattgaa aggtaagatg tagcccacat
    11461 aaaagtttgg ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa
    11521 tatgatggtt ctctaagata actttgcggt atttatggac aaatcgttcg acaaggtcat
    11581 ccgaatcttt tttagaggtc ttgatctcaa taagtaattt ttggtttagc ttatgtgctt
    11641 tttccaaata agaatcaaaa gaacaaacag gggcttgcac tccgttttca gtaactgtaa
    11701 gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc atttacccct gttaagtgac
    11761 gaagatttgc atcatgcatc actacgaaat gaccatcttt tgtttcctga acatccatct
    11821 ctacataatc aggatgatag tctctgcttg ttttttcaag tgcttctaaa gtattttgcg
    11881 ccccattttg ggtactcaca cctctgtgag agacagtcac tggttttgtc tgaacagctg
    11941 aagtcagata atccatattg taaagacaga cgccaatacc gaaaaaaact gcaaaaagag
    12001 tcaaaccagt gtttttcagc gcagagaaac gcaagttggg ggcttgtggg ataaaccact
    12061 tagggatctc aggcaagaag ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc
    12121 ctacggtaga catcacgata ttgaataata aaatcccttg taaaagcgtc atagcaaaca
    12181 cagcgctgat caatgcatag tctgggaaaa attgttcaac catactttgt gccaaaataa
    12241 caacgatata tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca ataaattggc
    12301 ccgtgatggc caaaagacgc gatttggtca atgaccagct ttcccgaatg gctgctcgaa
    12361 aagaacgatc tcgtagaatc atttccggta aagcaaaaat cagtcgaatc ccaatatagc
    12421 caaggaaaat ataaacgagt aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa
    12481 aatccataat gaaagcgggg atttttattt ttgacagtag atcagaatta aaacttaatc
    12541 cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa caaaaaagta atgggtctca
    12601 ctttcttcag ctgaagtatc gtcaagtgta agagttgctt caaagagatt ggttctttct
    12661 ttttaataaa aaaaacactc atcaaaagaa aagtaaattc gaaataaact aacagtaaaa
    12721 tcgtcatcaa aactacgatt aacgagagta gaaccccagg atgttgagaa aaaattgaag
    12781 gaatattgtc ataagacaaa tagtcaattg cccctcggtg taaaataaaa cgggtcgtac
    12841 tgctcaataa gggtaaacaa ataaataaaa taaagccatg catcagcaag acatcacgga
    12901 aataagcttg tgttcctttt aaaaagtccc aagcattacc taagctacgt ttcaaataat
    12961 tcatcctatc acctgctctt cacattgtag cgcaaatcta atgaaaaagg aaagggtgca
    13021 gtgtgttttg cagacccttt cccagattta ac
//
LOCUS       DBNIBO010000075        13050 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.079, whole genome shotgun sequence.
ACCESSION   DBNIBO010000075 DBNIBO010000000
VERSION     DBNIBO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.079"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="AC1Y77_001710"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="AC1Y77_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIQ1575283.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1874..4540
                     /locus_tag="AC1Y77_001711"
     CDS             1874..4540
                     /locus_tag="AC1Y77_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1575284.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4904..5656
                     /locus_tag="AC1Y77_001712"
     CDS             4904..5656
                     /locus_tag="AC1Y77_001712"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1575285.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5678..6832)
                     /locus_tag="AC1Y77_001713"
     CDS             complement(5678..6832)
                     /locus_tag="AC1Y77_001713"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIQ1575286.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7017..9458
                     /locus_tag="AC1Y77_001714"
     CDS             7017..9458
                     /locus_tag="AC1Y77_001714"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIQ1575287.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9788..10492)
                     /locus_tag="AC1Y77_001715"
     CDS             complement(9788..10492)
                     /locus_tag="AC1Y77_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575288.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10679..10924)
                     /locus_tag="AC1Y77_001716"
     CDS             complement(10679..10924)
                     /locus_tag="AC1Y77_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575289.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10941..11792)
                     /locus_tag="AC1Y77_001717"
     CDS             complement(10941..11792)
                     /locus_tag="AC1Y77_001717"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIQ1575290.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11958..12962
                     /locus_tag="AC1Y77_001718"
     CDS             11958..12962
                     /locus_tag="AC1Y77_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIQ1575291.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4169 a         2317 c         2716 g         3848 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgcctt ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgta ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcgacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt tcagacagga ctagggccaa gatcaaaaaa
     1861 tgggggatga aatttggaaa acaaggaaca agcacaacaa gcggtctata aagaaacgat
     1921 cgactcgttg atgcagcaaa cgcagagctc aaccgatgga ttgagcacca cgcaagcgaa
     1981 ggaaaaatta gaaagagatg gcttgaacca gctaaaagaa aagccgaaga agccaacttg
     2041 gaaattgttc ttagaaactt tcaaagatgc aatggttatc gtattgctga ttgtcgcact
     2101 gattcagatg gtcatgggtg actacatcga attcttagtt atttttgcag tattgatgtt
     2161 gaattctata atcagtgttg tccaaacaaa aaaagcagaa ggttctttag aagcgttgaa
     2221 aaacctttca gcgcccgatg ctcgagtgat tcgtgatggt aaggaaaaaa cgattccggc
     2281 aaacgaactt gtggtaggag atatcgtgtt gttagacgca ggcgattatg tacctgctga
     2341 tggacgttta ctagatgcgg gatcattaaa agtagatgaa ggtatgctga caggagagtc
     2401 gacttctgca gaaaaagaag taacggatat cacaagtacg gtaccaatcg gagatcgtac
     2461 aaatatggta ttcagtggaa cgatcgtaac ttacggacgc ggtaaattct tagtgacttc
     2521 aactgggaat aatacggaga tcggacaagt tgctggcctt ttggaatcaa caactgagca
     2581 agtcactccg ttacaacgag gtctggataa attcagtaaa aaactaagtc tggctatttt
     2641 ggcattgtct ttattgattt taggtatcca actgtttcgt atttatcttg gagaaggtac
     2701 aggagatatg actgcagata tcgtcagcgc agtgatgttt gccgtagccg tagccgtagc
     2761 tgctattcca gaagcattac agtctatcgt aacaatcgtg ttatctttgg gaacgaataa
     2821 aatggcaaaa cgtcatgcca ttattcggaa gcttccagca gttgaaacac ttggttcgac
     2881 aagtatcatc tgtacagata agactggtac attgacacaa aacaaaatga cagttgttga
     2941 ctacttttta ggtaatggcg atactgggga tttcacaaat gacccttcaa aatggacagc
     3001 tgacgaacgt cgcttgatcg aaatcagtgt tttagcgaat gatgcatcga tcagtgaaga
     3061 cggtactaaa ttgggcgacc ctacggaagt agcgtttatc gactttagtg aaaaattaaa
     3121 ccagccttat caggaaatcc gaaacaacta cccaagacaa gcagaacttc ctttcgattc
     3181 tgatcgaaaa ttaatgtcta ctggacacac gatcgataac gaaaattact tgtttgtcaa
     3241 aggcggacca gatatcgtct tccaacgcag taaaaaagta ttgctaaatg gtgaagtcgt
     3301 tccgatgacg gatgaattga tgaaaaaatt ccaagagcaa aatgaagctt tttcaacacg
     3361 cgcattacgt gtactagctt ttgcttaccg tccacttgat aattttgatt tatcatttga
     3421 agatgaagac gatttgatcc ttgttggttt gcttgctatg atcgatcctc cacgtgctga
     3481 agtgactcag gcggtaacag aagcaagaag tgcaggaatc aaaacaatca tgattactgg
     3541 tgaccacaaa acaacagctg ttgctatcgc aaaacaaatc gggatttttg aagaaggaaa
     3601 cctggctttg accgggacgg aactagatga attgacagaa gatcaactaa acgaagtttt
     3661 agaaaaagtc actgtttatg cacgtgtttc acctgagaat aaaattcgta tcgttcgtgc
     3721 ttggcaaaat aaaggtcata tctcagctat gacaggtgat ggtgtaaatg acgcaccagc
     3781 attgaaacaa gcagacatcg gaattgctat gggaacgggt acagatgtgg caaaagatgc
     3841 agcagcgatg gtattgacgg atgataactt tgcttctatt gtcagcgcag tagaagtagg
     3901 acgtaatgtg tttgataata tcaagaaagc tatttcttat ctatttgcag gaaatttagg
     3961 tgcgatcatt gcgatcatct ttgccttagt tgctgactgg tccaatccat ttacagcatt
     4021 acaactgttg ttcatcaatt tagtcaatga ttcactgcca gcaattgcat taggtacaga
     4081 aaaagctgaa cctgctacga tgaaccgccc gccaagagat ccaaattcag gaatctttac
     4141 ggacaaaaca ttgatttctg taacgtatcg tggtgtgctg attgggattg ctgttatctt
     4201 tgctcaatgg cttggcatgc agcattcacc tgaattcggt gtagccatgt cattttcaac
     4261 attgatttgg tgtcggacat tgcaaacatt cccagcacgt tcaaacacac aaacagctat
     4321 ccaagcaggg ttcttctcta acagaagtgt cttactagct gtagttgtat gtagcgcgct
     4381 ttactgctta acaatgatcc caggactgaa agaagtcttc tctatcccat cagcatttgg
     4441 attgacagaa gtctttacat gtatgggaat cgcacttgtg acaattgttt tgatggaaat
     4501 cactaaattg atcattgtac gagtacaagg aaataaataa aacgaatcaa cgaggttgtg
     4561 agcctgccgt ttaattctga gtcacaaggt gcaatttcaa caaacagctt ctcatgtttg
     4621 ttgaatattg tgacttgtga ccgaagagtt ggcaggagaa caccgtttaa ggaagaagtt
     4681 gtaaaatcag cattttgagc taatactgag ggtcatctga ttggacactg tttattcgga
     4741 aagagggact ttgacacgac tttggtcaca gtcccttttt ttatttatga tagacggaaa
     4801 gccaatcatt ttattattca tcaaaagaga tagaatagat aaaagaggag aataatttat
     4861 tccgaaaagt gaaaacatat aacaaaaaat tagaggaaat caaatgaatg aaaaagaatg
     4921 ggatgaattt gctgaagaat attatgaaat ccagcaagaa tcgcaagtaa ctattgctga
     4981 agatgtaaaa aattatctga aaacggagaa agtcctgcct gctgaaacag cagcagacgt
     5041 tgctggagga tctggcagat ttctttcctt agcaaaagaa attaatcgct atgatttgat
     5101 cgatttttct tcaggaatgc tagaaaatgc aaaaaaagaa gcagaaaaaa atgagttgcc
     5161 aaatgtttcc tttatcaaac agtcgttaga agaatttatc caatcaaatg agacatatga
     5221 aatggttttt acagcagcaa atccagcatt ggtacatctt attcaattag aaaaattgat
     5281 gaaaaagtca agcaaatggt gcgtgataat cagggtagtg gaatcaatcg atgatgtttt
     5341 ttatcctatc gaaaagcaac taggaatgat agaagaagat cctaatacat cgccagttgt
     5401 aatggatcaa tttgaaacat acttaattga tgaaggattc aaagtgaaga aaaaagattt
     5461 tacttatttt acacaggaat caattactga agaattgatg aagctatact atgacgcgta
     5521 cgcaaaagat cctgttgtga ccaggtacat tcgcaaacta ttcagtgaaa aacgagaaat
     5581 ccttagtacg actaagctga cctatagact gctcctttgg tccgcagata aaaaaagaac
     5641 ctttcacgac tcgtgaaggg ttctttttat gattttatta tattttgtag taacgaatat
     5701 cattttcgat ctttgtaatc gaaaaaggta catctgaagt atattctgca cattctccgt
     5761 gttcaggaag cgtctcactg aaaatcgttt catattcgtc aacagtgatc cgtgttcgag
     5821 aatctagcat ctcttgatga gattgtgcga agagatattc ttgatagttt tcttccagat
     5881 aacctgtaaa aaattcactt actgcaccag aaccgtagct gaatagaccg atgcgatctc
     5941 ctggctgtag tgatttcgaa ttttccagta gagaagttag ccccaggtat aatgaaccag
     6001 tgtaaagatt accgattcgt cggctgtaac ggatgctttc ttcatagcga gccataagac
     6061 gttcctgatt ttcttcgtct gtttggtcta atacgctttg caatgccttt tttcccatct
     6121 tagtatacgg aatatggaaa gcaatcgcct gataatcttc gagtcctcga cccgacaatt
     6181 ctttatgtcg attccaaact ttttggaatg attcgatata cgtagaatta gataaaggac
     6241 catcaacaac aggaaattcg ctataatctg gacgccagaa atcatagata tcttctgtca
     6301 gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt ttgagtgatc atcatcgcaa
     6361 cagcaccgac accttgcgtc acttcaccac cgcttgccaa gccgtaacga gcaatatcac
     6421 ttgctatgac tagtactttt cgttctggat gatttttgac atattctttc gccatgtgca
     6481 aagctgcggt gccaccgtag caagcctctt ttacttcaaa agaacgagca aaagattgga
     6541 ttttcagtaa atggtggata attaccgcac tcgcttttga atgatcaatc ccggattctg
     6601 ttccgacgat gaccatgtcg attagctcac ggtctttttc tgttacgatt ttgctggcag
     6661 catttgctcc tagtgtaatg atgtcttcat ttgcacgatt gactgccatt tggtcttgac
     6721 caatccctat atggtatttt gcaggatcat ccccacggct ttctgccaac tctgtcatat
     6781 ctaaatataa attaggaata aaaaaggaaa gacgatcaat ccctattttc atttcaacac
     6841 ctctttactt atgataaaaa acttacttta ctaaggtatc ataaaaagat taaaagaaat
     6901 ataaaaaaga gaatatttaa ggattgttaa aatttaatct tagaaaaaaa caactggaac
     6961 aagagctatt tgtttttaca gcaaagaatg gtatattttt gtaggaggtg gcttacttga
     7021 aagaagtcgt tatgatagat gctgcaagaa cgccgatagg aaagtatcgc ggaagtttaa
     7081 gtccatttac tgcagttgaa ttaggcacac ttgtgactaa aggattatta gataaaacaa
     7141 aactgaaaaa agataaaatc gatcaagtaa tattcggaaa tgtcctgcaa gctggtaatg
     7201 gtcaaaatgt tgccagacag attgctttga acagcggtct ccctgtagat gtcccagcaa
     7261 tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt gatcttagca cgccagctga
     7321 tccaattagg tgaagcagaa cttgtgatag ccggcggaac agagagtatg tctcaagcac
     7381 ctatgctgaa accgtatcag tcagaaacaa atgaatatgg tgaaccaatt tccagtatgg
     7441 tcaacgacgg attgactgac gcattttcaa atgcacatat gggattaacc gcagagaagg
     7501 ttgcaacaca attttctgtg agcagagaag aacaggatcg ctatgccttg tcgtcccagt
     7561 tgaaagcagc acatgctgtc gaagccggtg tattttctga ggagatcatc ccagtcaaga
     7621 tttctgatga agacgtgtta tctgaggatg aagcagttcg tggaaatagt acattggaaa
     7681 aactgggcac gttacgtaca gtattctcag aagaaggaac tgtaacagca ggaaatgctt
     7741 cgccattgaa tgacggtgcc tctgtggtga tccttgcatc caaagaatac gcagaaaata
     7801 ataatctgcc ttatttagca accatcaaag aagtagcgga ggtcggtatt gacccaagta
     7861 tcatgggaat cgctccaatc aaagccattc aaaaattgac agatagatca ggaatgaatt
     7921 tatcaacaat cgatcttttt gagatcaatg aagcatttgc agcttcttct atcgtagtta
     7981 gccaagaact gcagttagat gaagaaaagg tcaatatata tggaggagca atcgcattag
     8041 gccatccaat tggtgcaagc ggtgcacgta tcttgactac attagcttac ggattgcttc
     8101 gtgaacagaa gagatacggt attgcttcat tatgtattgg tggaggttta ggcttggcag
     8161 tcttgttaga agcgaatatg gagcaaacac ataaggatgt gcaaaaaaaa aagttttatc
     8221 agcttacgcc atctgaacgg cgcagtcaat taatagaaaa aaatgtattg acacaagaaa
     8281 ctgcgttgat ttttcaggaa caaacacttt ctgaagagct ctccgatcac atgatcgaaa
     8341 accaagtgag tgaagtggaa atcccaatgg gcatcgcgca gaattttcaa atcaatggta
     8401 agaaaaaatg gattccgatg gcaacagagg aaccatctgt cattgcagct gcaagtaatg
     8461 gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca gcggctgatg cgagggcaaa
     8521 tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa tgcagttaat catcgaaaag
     8581 aagagctgat tctttgtgca aatgaaagtt atccttcaat cgtgaaacgc gggggcggtg
     8641 ttcaagatat ctctactcgg gaattcatgg ggagtttcca tgcatactta tctattgatt
     8701 ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat caactcgatt cttgagagtg
     8761 tggcaaacaa gttacgggag tggttccctg aagaagagat tctattcagt attttaagta
     8821 attttgctac agaatcattg gcttctgcct gttgtgaaat cccattcgaa cgcttaggga
     8881 gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca acaggcaggg gaatatgcga
     8941 agcttgaccc ttaccgtgca gcaacgcaca acaaagggat catgaatggt atagaagctg
     9001 tagtggcagc tacaggaaat gatacacgtg cggttagcgc ttcgattcac gcctacgcgg
     9061 caagaaatgg attataccaa ggattaacag actggcagat caaaggggat aaactagttg
     9121 gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg agcttccaat atcctgccaa
     9181 aagcaaaagc atctttagct atgttagata ttgattcagc caaggaacta gcacaagtaa
     9241 ttgctgcagt agggttagct caaaacctgg ctgctttgcg tgcattagtt acagaaggaa
     9301 tccaaaaagg ccacatggga cttcaagctc gttcattagc gatctcaatt ggggctattg
     9361 gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc agaaaagatg aaccagcaga
     9421 cagctataca aatattggaa aaaatccggg aaaaataaaa aagcaatatc tttagaagaa
     9481 taaagcaaga tttacagtta ttttatttac tgcaaatctt gcttttttct ctatcagaaa
     9541 atatgtttat tattagaatt ttttatagtt aaggttaggc atcttaagaa atcaggcaac
     9601 tccaagatat cttcaggtag aagagaagtc gcctaacatt gtaggatcat aggtgatttg
     9661 ttggacaggt caagctgata tttgagcctg atgttagaag gaatttcttt ttacttgctg
     9721 tagaaattat tatatgacta aaaaggctgg gattttaaat cccagccttt tatgacaaat
     9781 agaattttta tcgatcagta atcgctgtcg ctgcaaattg ttcgatcaaa ggaatcaaag
     9841 tcaatttgac tttgtatttc cgttcgagtg ctttttcgat aacgaactca ttttcagtag
     9901 tcatagaaat agttgctaat ttacgaggtt catcagcatt tttttcatta ataactactt
     9961 catgttcatt ttgttctgtg taaaatttag gatactcagc aacggctttt gaaaaatcag
    10021 caaaacttaa agcggcattc gtatcagttg agcgtgtatt ttcagttgtg tccgcttttg
    10081 gtttttccgt tttttcttct ggcacactga ctgtttcttc tgtaactggt gtcgcaacag
    10141 cttgcgcagt tggttcatcg tttttcaatc gttgatcatt ccagtaagac acgatttcaa
    10201 taagaagatt gtcacggaat cgttcgaaaa tcccgttttc tcctgaacga attctcttta
    10261 atacagtatt gaagttgtca tcgattggca cgttgatccg tgtatcttca tttgttttcc
    10321 cagctaatcc gatcgttaag atttgacggt ttttgggatc aagcatgact tgtaacggca
    10381 aaccatagcg tcgcaagctc tcttcttcta gagaacggaa taattgtcct cttacaccat
    10441 ccaatgaagg acggaattga tttaataatt tttgtttctg ttttttcttc atatgttctc
    10501 tccttcgctc ataccctttc gattataaga aaaaaacagc caaatgaaaa ggagtaatgc
    10561 aaaaaaactt gcgtatacgc aaattacaac agaaaaattt gcgtattttc ccgttttttg
    10621 aaagaaaaat aaaaaatgga acggattatg tatatataca taatccgttc cattctttct
    10681 attttttcgg tatcaaggca aaaacaggta gttcaggtga ataaatgact tcatgttcgt
    10741 gccagggttt gaccattagc gaatgatcga accgtttttt cagttcttcg atgatttgat
    10801 catgtgtcca gtttcttgca ggaaaatgtt ggattttctc tgcatcgatc aaaaagacaa
    10861 aatcggaatg aaaagtctgg gtaacttctt ccgagagaga attcaagcgt tcgattggat
    10921 ccataaaatc acctttcgac ttaaaattgg gtagtatctg ggtcttttgc tttgccgact
    10981 acaccatcta actgatcgat ttgtttcatt tcttcttctg ttaattcaaa atcaaataat
    11041 tcagtatttt ctttgattct gcttggtgtc actgacttag gaagaggaag gaattcgtgt
    11101 tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt catgtgcttc agcgatttgt
    11161 ttcatttcag gcacatcgaa gattttacca gtgcctaatg gactatatgc ttcgaggatc
    11221 atatcatgtt tttttgcata ctcaactaca gttggctgta attcgcctgg tgcaaggaaa
    11281 acttgattga ccatcggcat aatttttgct gtttttgcta gtgtatctag atggtggggt
    11341 aagaaattgc tcacaccgat tgctttgatt ttacctgctt catacagttc ttcaaatgct
    11401 cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa aagcaacagg gtttggccag
    11461 tggatcaaaa acagatctaa ataatctgtt tggagcttct tcaatgattc ttcaaaagaa
    11521 gacatcacaa gttcatagct gtggtttgca ttccataatt ttgttgtcaa gaatatctct
    11581 tctctaggaa ttccactttc cttaatcgct tggccgacac tttcttcatt cttgtatcct
    11641 tgggcagtat cgatatggcg ataacctgca gccagcgctt ctttaacaga ggaaacagcg
    11701 acatccccat caggtgtttg ccatgtgccg aatcctacta ccgggatttc gtaaccattt
    11761 aatagacgat aagtgcttgt taatgaattc atactttcag ctcctttttt attcgttcaa
    11821 ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt tgtttctact cttgaaattt
    11881 ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat tttttttccg taaactaaga
    11941 cagaagggaa tgagaaaatg gatatctatt taaaaaaagc aattctgcat attattgaca
    12001 gagaaactgg ttctccagtc ttttcacaaa aagagctgga tctgacaaaa gaatatattc
    12061 gtgattttct tcaaaagaag atccaaaaaa tctcttctgc tcaaacaaaa actggacaac
    12121 tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc tcaagaaaat tttgtggaag
    12181 caagtgaagc aattgttcag cggtggttcg atatctacca agaaagtgaa gaagcaccta
    12241 gtgcagatgt atttgttgtt ttgtatgaat tagatacagt gatgtactta tctttattga
    12301 aagtcaatta tcgtgaggct tacactcact ttgttgaagc agacgaagca gggatcgaca
    12361 accgcttgat tttgaatcga gcaattctag gggcgaaaac acaaaaagca gatgaagcat
    12421 tcgctgtgaa tttgactgat tggacctacg aattaattga aaaaaaatat gagttttctg
    12481 gcaaaaaaga gctttatttt tctggaagag tgatcgaaag tgttcctgca ccttctttag
    12541 aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct tggaaaaaaa tttgagacag
    12601 aagaatttga tatcatggct gatttgaaag aagctgtcta tgatacgata gaagaaaaag
    12661 gtcggttgga tcatgagatg attgcggaaa aagtcttcaa ggaaaacatc acggcaaaaa
    12721 tggcttttaa agaagaagta caagagcaag gatttgtccc agaagcacct cctgtaaaag
    12781 aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa attcaagcta tcaaacggga
    12841 tcgaactgat cgtaccagta gatgtatatc gcaacccaga tttgattgaa tttgtgaata
    12901 atccggatgg tacgatatcc gtgatgatca aaaatgtgga tgaagtattg aatcgtttat
    12961 aaaaatgact agaacattag gtgttcaagt accagtgatc agttaaatga attgtgtcaa
    13021 gagaaggatc ggaaaagacg aatataacaa
//
LOCUS       DBNIBO010000076        13033 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.080, whole genome shotgun sequence.
ACCESSION   DBNIBO010000076 DBNIBO010000000
VERSION     DBNIBO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.080"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(13..2052)
                     /locus_tag="AC1Y77_001719"
     CDS             complement(13..2052)
                     /locus_tag="AC1Y77_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIQ1575292.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDHGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2110..2667)
                     /locus_tag="AC1Y77_001720"
                     /pseudo
     CDS             complement(2110..2667)
                     /locus_tag="AC1Y77_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(2815..2981)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(3206..3412)
                     /locus_tag="AC1Y77_001721"
     CDS             complement(3206..3412)
                     /locus_tag="AC1Y77_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575293.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(3428..4042)
                     /locus_tag="AC1Y77_001722"
     CDS             complement(3428..4042)
                     /locus_tag="AC1Y77_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIQ1575294.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(4189..4872)
                     /locus_tag="AC1Y77_001723"
     CDS             complement(4189..4872)
                     /locus_tag="AC1Y77_001723"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIQ1575295.1"
                     /translation="MDTLEQIKGGVVVSCQALENETLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4981..5439)
                     /locus_tag="AC1Y77_001724"
     CDS             complement(4981..5439)
                     /locus_tag="AC1Y77_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIQ1575296.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(5523..7286)
                     /locus_tag="AC1Y77_001725"
     CDS             complement(5523..7286)
                     /locus_tag="AC1Y77_001725"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIQ1575297.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(7507..8967)
                     /locus_tag="AC1Y77_001726"
     CDS             complement(7507..8967)
                     /locus_tag="AC1Y77_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575298.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(9175..10101)
                     /gene="glsA"
                     /locus_tag="AC1Y77_001727"
     CDS             complement(9175..10101)
                     /gene="glsA"
                     /locus_tag="AC1Y77_001727"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIQ1575299.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(10124..11551)
                     /locus_tag="AC1Y77_001728"
     CDS             complement(10124..11551)
                     /locus_tag="AC1Y77_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1575300.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(11582..12796)
                     /locus_tag="AC1Y77_001729"
     CDS             complement(11582..12796)
                     /locus_tag="AC1Y77_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIQ1575301.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3744 a         2717 c         2177 g         4395 t
ORIGIN      
        1 aaaagcaatt ttttattgat aattttggtt gaggtattgc aatagttccg atgctcgttt
       61 ggttgctgaa tcatcatctt ctttattttc caacatgctg accatcatat aaccttggtt
      121 atcaagattg aaagcaaaca agaaactgtt ctcttgcccc tttacatcct gtttttcttt
      181 gatttccgct gtaccagttt tcgctgccaa cggaatccct aaagcagaaa gagaatgtgc
      241 tgtaccattt acatcttgca caacttctct cagatctggc acgatcgttt gtactgctgt
      301 ttcgctgatg acattcttct tatcttttgt ctctttatct gcaatcaatt taggatagac
      361 aagtgtgcca ttgttggtaa aaacagaata cattgctgct tgctggatag gagtaattag
      421 aagttcgccc tgtccatatc cagtatcagc tagcaagata tctgagttaa agctatcttc
      481 attagaaatt tgtgctggat tcatactaat tggcaaatca aggtcttcac caaaaatgaa
      541 tttatctaag cctgtacgaa attttttctc acccattttc aacgtttctt gtgccgcata
      601 tatattatcg gaatagatca aagcagtttt taagtctact tgtgatacat cactactgac
      661 acgcgttgtt ttaaaagatc cccaagaact gtctttttgc catttaagcc cgttgatcgt
      721 caacacttca tttggatcga tagtgccgtg gtcgagaccg attgctgctg taatcatttt
      781 aaacgtagat ccaggagcat aacctgtcgc aaatcggctg atgaatggat gttcaggatt
      841 ttcttcataa gctttgtaat cttcttgtga gatcccgttt gtcattttgt ttggatcata
      901 gcttggagag ctagcaagcg caagaagatc accggttttt ggcgttgtcg caacagttga
      961 tgcagctttt cctcctagac tgtcaaaagc tattttttgt gccttcgcat cgattgtcaa
     1021 tttgatatct ttgccatttt gaacttcatg ttcgatcaga acttttttct ctacaccgtc
     1081 tgcatctgta atgcttaatt tcccacctgt tgtcccacga agatccttat caaaagccat
     1141 ctccaaacct gagcgaccga tttttccatt actgcttaat tctggattct tatcaatatc
     1201 ttctgctgtg atatctccca cgtaaccaat cagctgagca gctgcttcac ccaaaggata
     1261 atatctgccg tctacttctt gaatggtagc tccagctgga agttctggcg ttgctccatc
     1321 aatgattttc aatgggacaa agtaatcggg ttgtacccag ctttgtgaaa tcgcctgatt
     1381 gatagcatct tctgttaagt cgaaggagga agcaatcgct ttgatattgg ctgttttttc
     1441 gcctccatct ccaagtttac ttggaacgac tcctaattgt tttagtttgc ctgttgttgc
     1501 taatggttca ccatttcgat ctataatatt ccctcttgct gcttcttgtg tggtcaggct
     1561 tactttgtca tttccttcca tttctggaaa aaccaagttg ggttgccagt tgatcgtggt
     1621 ttgaccatca tttctgtcta atgttccttt ataagaaaga tctttcaatt cacctaatga
     1681 ggtattcatc tttgctttat atgaaaaact gtaagtagaa tcgtcttttt tatctacttt
     1741 tagattcgat atctgaagtc ctttgacatc agcagcaccg tatatattct ggtatttatc
     1801 taagatctct ttttcagata atgcactttt atttgccgcc ttttttgatg tcatttctgc
     1861 agctttgtta taatctccct tgttcaaagc ttggacaaat tgctcaactg tcttttctcc
     1921 agcttctact gcttgggttt cttgatagtg ctggtagtaa aaataaccgc cacccgcagc
     1981 tgctattaag atcactgcgc cggcaatata agcgcctgtt cgatttttgc cgtgcttgtc
     2041 acttcttttc attttttcct ccttctcttg cttgtcattc ctttatttca ctatttatac
     2101 ctgtttattc taacatatgt ctttgaggaa atccatacca caatccagaa gcacactttc
     2161 tggaaggttc gtttggatac taccaagtaa tttcccaacc gctttgggta gcttgaatat
     2221 tgaagttacg tctttcagct gctgagagat agctttgatc acttgctgct gccttctgat
     2281 cctcccgaag tctccttctt tgtcttcccg aaatcgagca tactggagta aagtattacc
     2341 atccatcact tgctctcctt tgtttatata tacgccatct aaatctagag atttttctgc
     2401 atcgatcttg actccaccag gaaacagttc attaacgcaa tcgataaaat cttgaaatgt
     2461 gatacttgca taatagggag cttcaaattt gaaattttcc tttaatgttt gatttaataa
     2521 atcaactccc ccataagagt aagctgcatt tatcttattc attccgtaac ctggaatatc
     2581 gacatatgaa tctctcatga tagagatcag ttttgcttgt tgtgttttct ggttgtattg
     2641 tgcaaccaga agtgaatccg agcgacctcc accaatcgat cttttttcac cgcatctcct
     2701 cctcacacag caatccactt gtcaggtgag gagcgagttt gtctcatcaa ataagtcagt
     2761 cacaaaaata taaagaaaag gtcagaaacc ttttcgaaca agaattctga cctagcatca
     2821 tctcatcgtc cgagttttag cactatacaa cgtaaagaaa acattcttag ctattggaaa
     2881 aagccttaat ccaacagttt ctgttttacc cattggcgac tttcgacgtt tctgggcaac
     2941 agcctatgtt tgtataggag cctcacctaa caaggttatt actatattct atttacgagt
     3001 tcatgataga cttttcctaa tgattaggca atggaatttg aaatgaaata gtgaaattta
     3061 acaatcttta atatttttta gaaaaaaata acaaataagt tgaaagaatc ccgttgcaaa
     3121 ttctttcagt tgcaccagga ttctttgata attttttagt tttatgtgtt tttttatatg
     3181 ttttttcact tttcggaata aatatttatt tgttaaagta attaaacaat ccgctaatcg
     3241 tttgatctgg catttctatt tccttaccat ctaaagtaac agtagtaacg acaaaatggt
     3301 cttgcaatgg cggaacttct aagtactgcc cttgcagaaa atcattcact gtttcatatt
     3361 cttttttcat ttcttttgaa tctgtcgatg gtttatagct tatttttaac ataactagtg
     3421 ctccttttta ttcgggacgt gcaatcgtga aaatttctcc tattgctgca taatcatttc
     3481 ctgccaaacg gcttacagca ttcaattttc ttggatcgat ccttcctgtc ttatcatcat
     3541 agactgtttc atttaaatga tatttctgta cttctagcaa aaataaatca gctgttactt
     3601 tatttttatt ccgtatctcg acatgttcat ataaggatgt ttcaaaacga acttttgctt
     3661 ctgtcaatgc tggtactgaa atgattactg aatcagttaa agtcaagtca gtcaggtaca
     3721 gttcgctttc ctcattaggc agattggctg ctgttttatt tacttcttct acgttgtcct
     3781 catcaaggat atggataaca gcttctcttg tctcaagaat attctttgct gtgtctttta
     3841 ctgcatcttg ttttctctgg atcgccaagg ataagatggg tcgattcgaa ctgacgatat
     3901 tgaagtaact gaagggtgcg atattcacta tatcttttgc tgatttagtc gttactactg
     3961 ctactggtct agggatgact gaaccaatca gcaacttgta attttcacgt tctgtcaatt
     4021 ctgaaggact ccgttcaaac atggtttcgc cttctttcta taaaattcgt taaaaagcag
     4081 atcttgaaga caaaatgaaa agtaatgctt ttttcatctt cttagaatct gctttaaact
     4141 atctatcaat ttttcttttt tgtcaagaat ctgtgccgtg cgtgtttttt agttatttgc
     4201 tttacttgcg gcttcgacat atcttttagt gatcaattgc ggacgggtga taatagatcc
     4261 aactacgact gaaaaagcac ctaattcata tgctttctct acttgttcag gagagtcagt
     4321 atgtccttcc ataattactg ggatatcgac ttgttctatg atttgattca ataaatcaaa
     4381 atcaggtgtc tgcgtctttc gtgaatattc tgtatagcct gctaaagtcg tagacaaaag
     4441 atcaaaacca tcttcttgcg cttgaagcgc ttcctcaaaa gtcgaaatat ctgccatagc
     4501 taaccgaccg tgttcgtgaa tcatttggac aagttcagca gttgtttttt ctgtcggacg
     4561 tttacgagaa gttgcatcca gtgcaatgat ttctgcttgc gtctccaaaa gttcttccac
     4621 ttctttttct gtcgcagtaa taaacacttc tgacccatcg taatctcttt tgataatccc
     4681 tattaccggt aaatcaacga ttcgcttgat ttcattgata tcttctgtcg tatttgctcg
     4741 aataccaact gctccgcctt cttttgcagc caaagccatt ctccccataa taaaagaact
     4801 gtgtagcgtc tcgttttcca aagcttgaca agaaacgacg actccgcctt tgatttgttc
     4861 cagtgtatcc acaatatctc ctccagtaat gttatataaa aatacagctt catataaaaa
     4921 tgtaaccctt ttctattcag gagacaataa tgagctattt ctttcagcag acctttctta
     4981 ctaaaagaaa attacttcaa attcttcgta tacgaccagc ttctcttctg taaattctat
     5041 gttcaacgct tctttataag catgtttaaa aaactcagca tggactttcc gccaataagc
     5101 taatgtccga tccccttctc cttctttcca agcatgctct ttcgtgactt cactaaaagg
     5161 caagatggtc acatttatat ttttgatgat acaaactggt tcattttttc cattcaggac
     5221 cacgtctaat tgaccaactt ttggtaaagg ctcattctct tgtttataca gatcgtaacc
     5281 acttgatgta ccagtcttta tcttttttac aactagttcg ctcaactcgt ctgccatttt
     5341 ttctgtatcc ccaaatgaaa agaaacctaa tttttctgat tgataactag gatatttttc
     5401 ttggaacttt ttgacatatt cttctacttg ctgatacata acatccaccc ctttgctttt
     5461 tataatgaaa aaaccatcca gatactagta taaacaatta tctggatggt gagcataatg
     5521 aattattgaa tattcaagac acgcagacta tcttcaacga tttgttttgg tgttaaatga
     5581 taacgttcat aaagctgccc tacaggaacg ctatccgtga attctttctc tgcaccaaaa
     5641 tttaatacgt tcattgaaga atcaccataa taacgactga tattttcgcc aaatcctcct
     5701 gacaaactac catcttctaa tgtgatgact aattcgtgtg tttcttttaa tttatctaat
     5761 gtttctacgt cattttctgt gatcgttcct ggattgatca acgttgcttc gattcctttt
     5821 tttctaaaag cttttactgt ttcttttcct aattcaagaa aaccgcctaa agctaaaatt
     5881 gctacttttc ctccattatt tactgtttga taggctggaa tactataatc atttgcgata
     5941 gctgtaccat gttctacacc atgtgtagga attcggattg ctacaggctg cgctgtttgt
     6001 tcgaacgccc aatctagcat agagatcata tcctcttttg ttgctggagc aagatatttg
     6061 atgttcggga tacttgataa gtaaggaatg tcaaatgttc cttggtgtgt tcggtcacta
     6121 gctgagatcg ttccgccacc gactaaaata atcactggat tctcgttgat cgctaaatca
     6181 tgtgataatt gatcatatgc ccgttgcaag aaagtacttg attggaagac gactggacgt
     6241 actccctgag aagccaaagc agaagcgaaa gtgatagagt gctgttcagc gatccccacg
     6301 tcaaaataat gatctggatg tttttcttgg aatgttttca gtccaaaggc gcctggtatc
     6361 gctgcattga ctgcggcaat aggagctgta cttgtttgca ttttcttatc caaataatcc
     6421 atgatgatca tattaaatgt ttctccagaa gttacttgct ttgcctcccc ggtggacaga
     6481 tcaaatggta catgccaatg atatttcatt ttattatcga ttgccggctg ataaccgcga
     6541 cctttttccg tatggacgtg aagtacaatc ggatgatcaa tatcttttac ttcttcaaat
     6601 aaacggatca ccgtttccag atcgtttcca ttatccacgt aacgataatc aaatcccatt
     6661 gctttaaata ggttattgct gcttttaccg ttggtttgac gaagctcagc taagttttta
     6721 tataaactgc catgattctc atcaatagac atctcattat cgtttactaa tacgattaga
     6781 ttggattgta aaacaccacc attgttcaat ccttcaaaag ctaagccacc acttagggag
     6841 ccgtcaccga taaatgctac cacatttcct gattctttct tcagatctct cgctttagct
     6901 aaacctgttg ctaagctgat agatgtagat gtgtgcccca ctgtaaaata gtcatgttta
     6961 ctttcatgtt ggttggaata tccgctcact tcacggtaat gtcctttttc aaaaccattt
     7021 tttcgaccag ttaaaagctt atgaccataa gactgatggg aaacatccca aacgatttta
     7081 tcctttggtg aatcaaatac ataatgaaac gcaatcgtta attctactac acctagattc
     7141 ggaccaacgt gcccacctac atcgctgtcc ttttgcaaga ttttttggcg catctcttct
     7201 gccaactgtt tcatttcctc aacagataat tttttgacat ctgcggggct attgattgca
     7261 tccaatacat gtaatactgt actcataata cattctctcc tccgtactat cttcttttaa
     7321 tgaaaataga tttgttacac tttatctatt ctactactta gagttgactc taagtcaata
     7381 gcattttaaa aaaatttcag agaaagcttg ttatataagt agatcttcgc acgaatcagg
     7441 actcaaataa aaaccttcta cagacaatta aaaagctttg tagaaggtct cctaattaag
     7501 gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc tgagcaatat gatacaaatg
     7561 gatgtttgca ccttttaatg agattggata gatctttgtc ttttcagttt ctaatccatt
     7621 ttgatagcta gcagcatcgc acaaaaccac tctttttttc gcacatagac attcatcagg
     7681 caattcttct aatgaacgaa ataaaatagg atttatttca gcgatgcctt cttccgcata
     7741 caaatgaaat tgggttcgat aaaaaaattg tttgactgaa ctttctgaaa caacaaaaat
     7801 ttctatttcc tgtctccccg cttgttcact gttgatcaaa gaaagacttt tgacgaattt
     7861 tcgaatgaaa ttttcattcc atcttagttg gatttgattt ttctttttcc actcttccaa
     7921 tatttctaca atttttccgt acagaagttg ttctttttct gatagaagat agatcctttc
     7981 cggtaaaaat acttgtccat ctgcccacaa cgtacggaca aattgtaaaa aggattgctt
     8041 gaaaataaaa ttttctgcat cgtcgatttc agcgatacta ataatctgta aaatcaattc
     8101 attcagtgat aggtcattga gaacaaaatt tttataaaca acttcgaaat cttttcttaa
     8161 cagctccatg ttctcattgg tataatttct actgttgaac aaagaaaaaa tataatagac
     8221 atcttcttct tgtaagtcta gaattccttt ttgctcttcc aatccttgac agactaattg
     8281 aaataacggc ttcgtttctg cgcttttctt atcttcttct ttcagtccaa ggggaaatgc
     8341 ctttcttccg attgcgatct ctattcctcg tatgataaag gacttttcat cttccgaata
     8401 tcttctttta aaaaaatgag tctctacata atcaatcaac tcgttacaag cttcttctac
     8461 atacggattt ttattttcat agccttccca attgacataa cgtactaaag ctagcaagat
     8521 gttccgcgag tcaaattctg ggataatgat ttctttattt ttcaaataat cattttcttt
     8581 aaaaaaatcc aatacgattt ttttgattgt atatactttg ctcaaagaaa taaattcaag
     8641 atcagccagt tctgtactcg tgaatatacc attaaaataa taacttaaaa actttaaaaa
     8701 ataagattga ccgtacagaa actgtgtgag ctcagaaaga gcaataccat gtttgaatac
     8761 gagtttatac ttatgcttct ctcttacttg ttcttttata caatattcta gacactcggc
     8821 tagactttct agatcgtgaa ggatcgttgg tgtagtcacc cttagagaaa tagctgtttg
     8881 ttcgatgtct atttcttgga attcaaataa ttgttcacac agatacactt ttctaaatat
     8941 atttcgctcg atgtaatttt ccagcattcc tgatacacgc ctccagtttt tcttatgatt
     9001 ttatttaatc atatctgtaa atcgtatttt tgtttccttt ttatttagaa aagccctttc
     9061 aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca agctcacaat ttcagcctca
     9121 ttgtaggtag ctttgagccg tttggacttt taccccagcc tctttcaaga atggttattc
     9181 aaagatgtca gcgtctagtt ctttgactac atctttcaac aattggatac cagctgcgct
     9241 gttaccaaat ggatctagtg ccgggctgaa gacacctatg ccgtaacgat ttggcacagc
     9301 tgccatcaac ccgccgccta caccgctttt tgcaggtaca ccaacatgga cggaaaactc
     9361 tcctgattca tcatacaaac cagcagttgt cataattgat ttgacgattg tagcactttc
     9421 ttcagtaatg atttgtttgc cattccaagg agcaattcct ttatttgcca gcacagcagc
     9481 taattttgct atgcctgtag cggtgacatt gagggagcat tgtctgaaat aagcatcgag
     9541 tatctccggt acgtctcctt cgatcatttg attacctttc atatagtaag caagagagcg
     9601 attgatatca cctgttcttg attcagaatg atagatttct tcattcaatg tgatattagg
     9661 atcatcacag atctctttca taaatgacaa gattcgctca aatttttcat ctgcatcttt
     9721 accggcaatc aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat tcattggatg
     9781 tttgcgatga ttgatttcca tattcatgat agaattgaag gcaaaaccgg ttggttccgt
     9841 gttgattgtt tgaaagactt tttctattcc gttgtctttg attgctaata acaatacggg
     9901 aacttttgac atgctttcga tggcaaagcg cacatcggcg tctcctccct caatcagttg
     9961 ttttgtatcc aaatcataaa tcgccatacc taattgtttg ggatccactt cagctaatgc
    10021 tggaatgtaa tccgccactt gtcctgtttg ataatagtgg aaattttttc caatcaattg
    10081 ttctaattct tttacttcca tttgaataac cagctttcat tttttatttt tctgcatgct
    10141 ggaatttatg acggcatgcg tagatcgtaa atggaataac taagacgata agataagaaa
    10201 caatcagaat cgtttcataa atcccatgct gtccagctgc gatactagca ggtggaacaa
    10261 atgagataca aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg attactttta
    10321 caacttttcc gcctggaacc tgataggctc tttcaagact gtcatcttta aaaaccagct
    10381 tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta gattacaaca gtcaatgaca
    10441 tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg gctgcccaga
    10501 ttgttgcaac gatcccattt gcgatcatca aatgagcagg aacaccgtgt gtgttttctt
    10561 ttgcaaaata ttcaggaagt aatccttctt ctgctgctac gtgaagtgct tttgaaggag
    10621 aggttaccca agaagcaatt tctccgatga cacctaaaca aacaagaatc gcaagaattt
    10681 tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag gaattctaac gattgaatca
    10741 cccctgtatt aagagacaaa ccatgttgag gtactgtagt agcgacggtc aatccgccaa
    10801 aagtatctaa aatgattgcc agaataacca atacaaacat tgctaatgga taatcctttt
    10861 tagggttttc catttcattt gcatgactgg cagatgtttc tacgcccata tatgctaaaa
    10921 taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc aatgttttca
    10981 tagacagtat cgtttgtagt ggagcgcctg aagcgacata atggatccct aagccaaaca
    11041 agactaatgc tggcaacaag ataccagcta caaaaccgat ccgagcaatc aatgaagtgt
    11101 atttggttcc ttttaactgt gagagtaaaa tagcccagaa aataatcaat aggattgcca
    11161 atttcagata tttattgttt tgtatagcac caatacccgt tgcataggat aatgcaccag
    11221 ttaagaaata cagcatggtc aagtagccaa ctgttatctc aaaccattgg aaaaagattg
    11281 cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca agtatagaca ccgccttttt
    11341 cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca
    11401 aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt
    11461 catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt
    11521 ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt
    11581 ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg
    11641 tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg
    11701 catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg
    11761 gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc
    11821 cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa
    11881 aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg
    11941 cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta
    12001 cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg
    12061 caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa
    12121 gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag
    12181 ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg
    12241 tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga
    12301 aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga
    12361 aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc
    12421 ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa
    12481 agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc
    12541 tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca
    12601 atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag
    12661 acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag
    12721 gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga
    12781 taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt
    12841 agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt
    12901 aataaaagct tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata
    12961 aacggtcttc tttttataga aaaccgttta ttttcgtctt tttttatttt taaagatatt
    13021 ttttcataag gaa
//
LOCUS       DBNIBO010000077        13005 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.081, whole genome shotgun sequence.
ACCESSION   DBNIBO010000077 DBNIBO010000000
VERSION     DBNIBO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.081"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(99..2225)
                     /gene="pnp"
                     /locus_tag="AC1Y77_001730"
     CDS             complement(99..2225)
                     /gene="pnp"
                     /locus_tag="AC1Y77_001730"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIQ1575302.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="AC1Y77_001731"
     CDS             complement(2413..2682)
                     /gene="rpsO"
                     /locus_tag="AC1Y77_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIQ1575303.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2933..3496
                     /gene="def"
                     /locus_tag="AC1Y77_001732"
     CDS             2933..3496
                     /gene="def"
                     /locus_tag="AC1Y77_001732"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIQ1575304.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3749..4270)
                     /locus_tag="AC1Y77_001733"
     CDS             complement(3749..4270)
                     /locus_tag="AC1Y77_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HIQ1575305.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4273..5115)
                     /locus_tag="AC1Y77_001734"
     CDS             complement(4273..5115)
                     /locus_tag="AC1Y77_001734"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIQ1575306.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5108..5833)
                     /locus_tag="AC1Y77_001735"
     CDS             complement(5108..5833)
                     /locus_tag="AC1Y77_001735"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIQ1575307.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6009..6305
                     /locus_tag="AC1Y77_001736"
     CDS             6009..6305
                     /locus_tag="AC1Y77_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575308.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      6561..6735
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            6873..8465
                     /locus_tag="AC1Y77_001737"
     CDS             6873..8465
                     /locus_tag="AC1Y77_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1575309.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8478..9218
                     /locus_tag="AC1Y77_001738"
     CDS             8478..9218
                     /locus_tag="AC1Y77_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575310.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9386..10735
                     /locus_tag="AC1Y77_001739"
     CDS             9386..10735
                     /locus_tag="AC1Y77_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIQ1575311.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(10863..11453)
                     /locus_tag="AC1Y77_001740"
     CDS             complement(10863..11453)
                     /locus_tag="AC1Y77_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIQ1575312.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            11600..12952
                     /gene="brnQ"
                     /locus_tag="AC1Y77_001741"
     CDS             11600..12952
                     /gene="brnQ"
                     /locus_tag="AC1Y77_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIQ1575313.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         3625 a         2581 c         2573 g         4226 t
ORIGIN      
        1 gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt ccccctgtca
       61 ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc atttttcgtt
      121 gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg tttatcgatt
      181 tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac tttattgaca
      241 cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat caagttgacg
      301 aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc aactttgact
      361 tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc atcagctgaa
      421 gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc atcgatgatt
      481 ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg ttcgatctgg
      541 atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc aatcgttgaa
      601 gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc ttcagttagg
      661 atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat accatctttt
      721 gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg gatatcagtt
      781 aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc gactggcgct
      841 ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact tgcttgggaa
      901 gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg aaattcttct
      961 tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc gatttcccga
     1021 cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa attgtagtgg
     1081 tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg ctctcctaga
     1141 ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa acctgatcca
     1201 tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc caatttacgc
     1261 ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt ttcaagatct
     1321 tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc tgcaaatttc
     1381 tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc gcgagcaagt
     1441 ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa gatttctttt
     1501 ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc tactgcttga
     1561 acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa cagcaatgca
     1621 ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt gatggcttgt
     1681 ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac tgttggattc
     1741 aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc gtcaaatggg
     1801 atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc tggcgagcag
     1861 tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa gccttctgca
     1921 aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc cgtacttgga
     1981 cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat tttttcttca
     2041 taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc cacagctgca
     2101 cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg ttttgctagc
     2161 tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac ttgtttttct
     2221 gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa atgaaaactg
     2281 ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct agcttgatca
     2341 cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga atcccactct
     2401 ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt aacgttgaac
     2461 gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt tcattaggcc
     2521 gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt ggttgatttc
     2581 ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat ggcgagcgta
     2641 ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca cctctttcga
     2701 ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg caggtcatgt
     2761 gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt agttaatcat
     2821 taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt ggtaaactat
     2881 aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt ccatgattac
     2941 aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa aagaagtttc
     3001 tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt tcttaaaaaa
     3061 cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg gcttagctgc
     3121 tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc ctgacccaga
     3181 ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga gccattctgt
     3241 tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag aagtaccagg
     3301 atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg gtgaaaaaca
     3361 caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg accatatcaa
     3421 cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa aagaaggcgt
     3481 attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa gaaagcttta
     3541 taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc actaatcgaa
     3601 atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg atacgcatct
     3661 tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga tagaatagtt
     3721 tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct gcttcagcta
     3781 gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta tgcagatgga
     3841 tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt ctcataaagg
     3901 aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg taaaggggat
     3961 gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc agttcttctt
     4021 ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta ttggtttctt
     4081 ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca gctcgtagta
     4141 aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca aacttgctag
     4201 cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc cgtgtctctc
     4261 cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt gtttcatcaa
     4321 atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca gactaacaac
     4381 tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt cttctttttt
     4441 cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa ccaactctcg
     4501 atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta gttcttcctc
     4561 tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg gaaaagattt
     4621 ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac ccggcgtgac
     4681 gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg cgtactccag
     4741 accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct ggacattcgg
     4801 agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg tacaattgaa
     4861 atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat tgataactgg
     4921 ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt gggatcgcag
     4981 ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca tacggtcggc
     5041 aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt ctttttcggt
     5101 tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca accgctccgt
     5161 cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg attactaaga
     5221 ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact tgaaaactcg
     5281 ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc tccatgactc
     5341 catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct ccgcatgctt
     5401 gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat tttcgacaat
     5461 gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc tttgcgtgca
     5521 aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga tgtgctattt
     5581 ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca aattgataga
     5641 atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc tgctccatcc
     5701 cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg gatacccctg
     5761 caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct tcgtctactg
     5821 ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg aacaaattta
     5881 ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga gcaatttatc
     5941 acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata aaagtgaggt
     6001 gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt atcgcctggt
     6061 tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat ggaaatcttt
     6121 gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca ttagccgtat
     6181 atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta atattagcca
     6241 tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag aaagcggcag
     6301 cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc tttttttatt
     6361 gtattacata catttttgtg tataattata ctatcgtttg caatggatag atgattcaca
     6421 ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt ttttcaaaaa
     6481 ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg tataaataac
     6541 tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa ccctgtggag
     6601 tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac ttttttgctt
     6661 ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt gatgctaagt
     6721 tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct ttttatatct
     6781 ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat ctagaccgct
     6841 cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt tcagttgccg
     6901 tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat gcagatgaaa
     6961 aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc ttcaactggt
     7021 ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt tacgccggcg
     7081 gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa ttagtcatcg
     7141 tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc gacgcgatca
     7201 tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc gatccttatt
     7261 acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa gcaaaaagtt
     7321 tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac tatactgtga
     7381 tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca gcaatgagaa
     7441 cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa ggtgtaacgg
     7501 caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat ggcttccaga
     7561 caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg gatatccaaa
     7621 aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt atggaccaag
     7681 cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc atttctgatt
     7741 tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga cttaccttgt
     7801 tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt ggtgtcttcc
     7861 gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc ggaaatgcac
     7921 ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa gcaatggtca
     7981 tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt gccgctttgt
     8041 ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg gggatttttg
     8101 cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat ggccaaacga
     8161 tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca ggaaatgagt
     8221 ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct gatttgttct
     8281 tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt acgatcgtcg
     8341 ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg gtagaaaaga
     8401 aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca aatttgcagg
     8461 aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta agaaaaagct
     8521 tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt gaagttgtga
     8581 cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc aatttactgg
     8641 aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct cctaaatata
     8701 atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac ctgttcaaca
     8761 atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa cgagataaag
     8821 aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat cgttatatcg
     8881 aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt gctcgggcat
     8941 tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta gaccctgaaa
     9001 tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg acaatgctta
     9061 tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt tttatggata
     9121 aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg aaagaagaac
     9181 gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg attgggacaa
     9241 agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg taaatttctg
     9301 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
     9361 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
     9421 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
     9481 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
     9541 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
     9601 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
     9661 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
     9721 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
     9781 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
     9841 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
     9901 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
     9961 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
    10021 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
    10081 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
    10141 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
    10201 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
    10261 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
    10321 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
    10381 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
    10441 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
    10501 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
    10561 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
    10621 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
    10681 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
    10741 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
    10801 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
    10861 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
    10921 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
    10981 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
    11041 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
    11101 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
    11161 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
    11221 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
    11281 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
    11341 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aataactctc
    11401 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
    11461 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
    11521 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
    11581 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
    11641 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
    11701 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
    11761 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
    11821 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
    11881 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
    11941 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
    12001 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
    12061 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
    12121 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
    12181 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
    12241 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
    12301 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
    12361 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
    12421 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
    12481 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
    12541 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
    12601 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
    12661 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
    12721 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
    12781 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
    12841 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
    12901 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
    12961 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttg
//
LOCUS       DBNIBO010000078        12994 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.082, whole genome shotgun sequence.
ACCESSION   DBNIBO010000078 DBNIBO010000000
VERSION     DBNIBO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.082"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(118..1227)
                     /gene="rpoD"
                     /locus_tag="AC1Y77_001742"
     CDS             complement(118..1227)
                     /gene="rpoD"
                     /locus_tag="AC1Y77_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIQ1575314.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1251..3092)
                     /gene="dnaG"
                     /locus_tag="AC1Y77_001743"
     CDS             complement(1251..3092)
                     /gene="dnaG"
                     /locus_tag="AC1Y77_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIQ1575315.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3274..5589)
                     /locus_tag="AC1Y77_001744"
     CDS             complement(3274..5589)
                     /locus_tag="AC1Y77_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1575316.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5774..6634)
                     /locus_tag="AC1Y77_001745"
     CDS             complement(5774..6634)
                     /locus_tag="AC1Y77_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIQ1575317.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6693..7325)
                     /locus_tag="AC1Y77_001746"
     CDS             complement(6693..7325)
                     /locus_tag="AC1Y77_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIQ1575318.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7492..8253
                     /locus_tag="AC1Y77_001747"
     CDS             7492..8253
                     /locus_tag="AC1Y77_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1575319.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8279..10870
                     /gene="mprF"
                     /locus_tag="AC1Y77_001748"
     CDS             8279..10870
                     /gene="mprF"
                     /locus_tag="AC1Y77_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HIQ1575320.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11058..12245)
                     /gene="tuf"
                     /locus_tag="AC1Y77_001749"
     CDS             complement(11058..12245)
                     /gene="tuf"
                     /locus_tag="AC1Y77_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIQ1575321.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12613..12969
                     /locus_tag="AC1Y77_001750"
     CDS             12613..12969
                     /locus_tag="AC1Y77_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1575322.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3726 a         2717 c         2309 g         4242 t
ORIGIN      
        1 tgaatgaatt tccttcctat agaaaaaaaa gcttgagact gcgtagtctc aagctttttt
       61 tcttttaaaa taaaccaaac agttttactg atttctcttc tggtcaatag ttcatgctta
      121 ttctaagaaa tcttttaatt gttttgaacg agaaggatgg cgcaattttc ttaatgcttt
      181 agcctcgatc tgacgaatac gttcccgagt aacaccaaat actttcccta cttcttccaa
      241 tgttctcgtc cggccatcat ctaaaccaaa acgtaagcga aggacatttt cctcccggtc
      301 agtcaatgta tctagtacat cttccaattg ttcttttagt aactcataag ctgcgtgttc
      361 tgctggactt gttgcttcct gatcttctat gaaatcacct aagtgagagt catcttcttc
      421 accgattggc gtttcaagtg aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt
      481 ttctgttgga agatccattt ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc
      541 ttgcagcaat tgacgttgga tacgtatcaa tttattaatt gtttcaacca tatgaactgg
      601 aatacggatc gtacgtgctt ggtcagcgat tgctcgagtg atggcttgac gaatccacca
      661 tgtagcatag gttgaaaact taaatccttt gcggtagtca aatttttcaa ctgccttcat
      721 cagtcccatg tttccttctt ggatcaagtc aaggaactgc atgcctcgtc ctacataacg
      781 tttagcgata cttactacta aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg
      841 gtcgccttct tcgattttca atgctaacgc tacttcttct tccgcagtta aaagggagac
      901 acggccaatt tctttcagat acatacgaac cgggtcattg atttttacac cggttggcgc
      961 agatagatct tctgtttttg cttgttcagc ttttttctca gcactcttca agctgtgaat
     1021 tgatggatca ccattttcat ccaccacact gatacctgcg tcttcaatct tttggatcaa
     1081 tttgtccatt tcatctgcat tcaaagtgaa tggtgtagca agctgattgg ataaatcatc
     1141 atatacaact tgtcgtttgg gtttgttttc tttgatgaaa gctgctactg ctgcttcata
     1201 cgttttttta tctgttccat ttgccataaa agaaggcccc ccttgagtag ctatttggct
     1261 tgttttaact gtttcgttaa attgatgatt tcaatcgtca attcaagctc aagctgtttg
     1321 tttccagttc gtctggcttc ttgctgcatt tgttttttta atgttatttc atcagctaag
     1381 ccagatttgc tgatcacatc tagcaagtct tggatttctc gtggcgaact ttcttctgcc
     1441 accggtaaat attcgatttc caccaccaac cgccttaaat tttcatcttg caaaaagtct
     1501 aaaaactctg caaggttgaa ttcataatgt tgtgagacat atgcatcgaa taaaaaataa
     1561 acttctgcat atgcatcatg aataaactga aattctcgtt cttttaaaat attacggaca
     1621 gaaacttctt taaatgcacg atataataga atctgctctg ccttttctac ttgcgtcaaa
     1681 ggtctctttt tccgtgcagt ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt
     1741 tcttgacgat tttgctgatt tgtccttcga agatcgcgca gttgtttttg tatcgtctct
     1801 ctgccgatct gaaactctgt tgacagttga gatagataca tatcttgttc gattggtgaa
     1861 acaactcggc taagttcaac tagtaattct tcgaggtatt cgatcttgtc tttttcatta
     1921 tctaaatttt tcccttgttt taaataacgt gatttaaaag taaagactgt ttcgcgccca
     1981 tgagccacta attcttggaa agatgcttct ccatacttac ggagatattc atcaggatca
     2041 agcttttctg gaacgactac gacagaaagt gctaagcgac tgttttgccg aagcaattca
     2101 atccctcgat tggttgcttc aaaacctgcg ttgtctccgt catagcaaaa gaccaattct
     2161 ttcgtcagct tttccatttg ttggatctgt tgagctgtta gacttgtccc catagaagct
     2221 aatccagttt tgataccagc ctgccatgca gcaatcacat ccataaaccc ttcgaacaac
     2281 aaaatttctc cgtttttccg gatatcgctt cttgctttat caagattaaa taaaacttgt
     2341 cttttattga acagttctgt ttctggacta ttcaagtatt tgggctgatc tttttcctgc
     2401 cctttttcag gtgcaagcca gcgtcctgaa aaaccgatcg tctggccttg agaattacga
     2461 agtggaaaca tcactctttg ataaaaacga tcagacatac ggccatcatc atgcgtgaca
     2521 aacaatccag agcgttctaa caattcattt gaaattgatt cattcttgaa tacttgctga
     2581 agaaaagaac gctcattagg tgcaaaacct atgttgaatt cttcaattaa ttctaaagac
     2641 aacccacgac tttgcaaata ttctaaagct ggctgtccag cttttgtatg caatagcatg
     2701 tgatgataga tttccgctgc tttttcatga gcagcaatca gtttccccgt agcagattca
     2761 ggattttgga cagctatagt acgctgacgc cattgatctt ctaacggtat ctgttcgaag
     2821 tctgccactt ttaccactgc ttctggaaaa cttagacctt ctagttcttg aagaaaagta
     2881 aacacgtttc cgcccttacc acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca
     2941 gaaaaagacg gtgtacgttc ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac
     3001 tttttcaact ggacatattg tccaatcacc tcaacgatat tcgtttgact gcgtactgtt
     3061 tcgataacct cttgaggaat ccgttgagcc atttttccac cccctgctct attgatatct
     3121 tttgggttta acagaagata tttcaggaga ataaccggta aaaaatcgca ctaactgaaa
     3181 tcagtgcgat tttaaaggac acaattatac atgtttgatt gtatcatatt tcattgcttt
     3241 ttcaagtata acacatttct atctcattaa gcatcaattt gctactcttt tcagtaactg
     3301 attttgaata tcagccttcc agtacaatac tcgaagaaca atatttacga gcatgaatac
     3361 aaagaagtat cctacgctct ctcccaaccc taagtttact tccaaaattc catggaacag
     3421 cattgcagct acatatatcc ctacgatcgt cgttactgct aggaagatgc gcaatgggtt
     3481 gaagggataa caagctttta caactgccat gcaactgatc ccaatcagca agtaatacat
     3541 caaagtagta gtttcagcat ttccccatgc gaatgttttt ccaagtagat acaccgcaat
     3601 gatattcact actacaagca aggcatttgg taaagcatta ataagtgctg taggcaaata
     3661 acggtaagtg atttttcttt tatcttgttc aaaagataaa aagaaggaag gatacccttc
     3721 aatcgctaaa tcaatcagcg tgatttggat agggataaac ggaaatccaa ttgctgtaac
     3781 ggcacatata atcgataaaa taaatgaata aatcgttttg ataaaaaaga tgcctgagac
     3841 tttcgtcaca ttgttgacca ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa
     3901 atcagagtcc aacaatacca gattcgcaat ctgacgtgtt gctccatcac cttctgccat
     3961 agcaatgcta caatctgctt ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat
     4021 tgcaaccgtt ctgccgcttt ctttcaattc atttaccagc aatttctttt gctgaggact
     4081 cactcgtcca aaaaccgtat agcgatgagc agcttctcgc acttccattt cttcagtaat
     4141 ttgagaaaga tcgacatacg catcataatt cggtaatcct gcacgtctag cgatattgga
     4201 gacagtaaaa ggattgtctc cagaaatcac tttcagatcc acaccttcct catgaagata
     4261 agctagtgtt tcttgagcgt tttttcggat tggatcatcg atttcaaaca gagcaaccgg
     4321 ctcaacaaat gctggttttt cttcgttcaa ttgctgattt ggagcgatcc ctaacataag
     4381 tacgcgatag ccattcgctt gggctgtttt tagactgtcc ggaagccgat cttcttccac
     4441 caaacgttca ggcgctccta cgtacacgac tcccagttct ggaaattcca tcgcgcccca
     4501 tttgcgatca gaagaaaatg cgactttgtt tgtcacatca taccgattag aaagctcaaa
     4561 ataatctctc atagcccgca tagtgatgtt gttatcactg ctttctgcca gatacgtccc
     4621 cataatttct gggatggctt tctcgtacag cggatgtagt gcttctaccc gctggatact
     4681 catttttcct tcagtaatgg tccctgtttt atccaagcat aatgtatcga catgggccag
     4741 cgtttcaatt gaatacatgt cttgaacaag gattcgtttt tttgccagtt tggttacccc
     4801 tgtcgttaat gcaatactta ttagcaatac caaacccttt ggaagcatgc ctaacaaagc
     4861 tgctgcagat gcaataacag aaattttgat agaagcatcg cggatgaaca gtgcttccaa
     4921 aaatagtatg atccccaaag gaataatcac gaaactagtg aatttggata ctttgcgaat
     4981 cgaatctaca agttcagaac gaatagcttt gtgagtcttt gcctcgctag tcaagcggac
     5041 agcataatta tctgcaccta ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa
     5101 gcttccagaa agcaattcag ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa
     5161 aagagcttca tttgcttctg cccgtccaga tataacctgt aaatcggctg gtatctgttc
     5221 tcctgctccg atttttacca aatctcctaa taccagctct ttagtagaaa tcgattgttc
     5281 tttaccgtcc cggatcacta gtacttctga ttgggaaaca atcgataatt tccgtacaag
     5341 atttcttgca tgtatttctt gataaattcc aataacgata ttgacgacaa taattgctaa
     5401 atacgccatg ttggaatacg cacctacaaa taacagacaa atcccaatac caaagttcag
     5461 aaaattgaat aacgtcaata tattatcgct gaatatttgt tttgtacttt ttgatgcatc
     5521 ttcctggtag tcattttgct ggccagaagc cattttctga actacctctt gctgtgtaag
     5581 tccattcatt ttctttttct ccattttccg ccaactttca aaaataatca cgtgtttaag
     5641 tctagcgtgt tccaagatta aaaacaactt gtttttataa aatttataga aaaacacacg
     5701 aatgatttta caaaaaagat gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat
     5761 tatcaatcta tctttactta tattgttcct ccaaacggct catccaccaa cctagtgctg
     5821 taccggctat aaaaagaaca ggacaagcta gtaccccagc aactcccaat cccgcgtaat
     5881 cggtaggaat gatcataatc gttgaagcaa aaactacacc taaaatgaaa tggaatagct
     5941 tgctatatgc ctttgagaaa atgtaatcca tgatttttga caatccaaga acacaaagaa
     6001 tcccaccaat tccgatcggc acaatcactt caagtcgtac ttctttaaat ccgtcagcca
     6061 tcgctttata caatcccata tacaccaaaa aattagaagg gctcaatcct gggacgatca
     6121 tacccaaacc aatcaatccg ccggcaatca gccatgttcc aaaattctga tcgacttgcc
     6181 cgtcaaataa ttgagcgccg tataataaaa acaaataagc cagaacaaaa gtaacaacca
     6241 tgatgatcac atcagaagta tctcttcctt ttttccctgc ttctttccat aacgcaggaa
     6301 cagttccagc gatacatccg acaaaaaacc ataatatcgt tgtttcatac gcacctaata
     6361 aaaaactgac taaaaaagaa agtagaaaga tgcctgtcac tccgcctaga ccaacaggca
     6421 gaaaaaacaa aacattttct ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc
     6481 gctcatacat gccaaagaca gctgctaatg caccaccgct gactcctggc aagataaatc
     6541 ctgaaccgat aaacattccc ttcacaaacc gtaacatcca gtctttggtt gtgtacccca
     6601 ctttttgatt ttccattttt tgcctttttt ccactatttg cgcactcctt ctttcattta
     6661 attttattca tgattgaaca tttcctgcta ttttacttaa aaactttttc ataggtctct
     6721 attatttttt cattcatttt gacaatatca ggataaaaca tgttacctcc attatgatag
     6781 agatagccgc cattgtaaag caaggactgg atccgccaat atcggtattg tttcccttcc
     6841 tcatttccta aaagtgggct cagcacatct ttagaatacg cttctgctaa ttctactgtg
     6901 ttttctccgc catgattttt tacataggca atataatctt tcccaaaatt atatgcttgg
     6961 acagctgtcc ataaatcgca tccttgtttc tttgcttcta ctatcatttc cgccaaatat
     7021 ttgacacctt gatcaatact gtcagctgga ttatcaatga tctcagcttc tccatgcaga
     7081 ctttccgagc tccgcatgag atctgtccct tttccttttg attcggtcaa tattatcgcc
     7141 ttgatcaaat tctcgtagtt ttccatatga taattttttg cagctgttgt tatttctatt
     7201 tgatagcttt ccactcgttt taccgaacga taagttcttc ctcctaaaaa caacaccgca
     7261 ccaataaccg tcagaatgat catgtaaaaa agaatacgga taatagataa tcgttttcgc
     7321 ttcatttcat cacctagtta ttttgacatc tttttttatc ttactatttc tttatcttct
     7381 tggctatttc ttccttgatt tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt
     7441 tttgtcatct tacgaaaatg atcgttgttt ttcagtaaaa acaattgccc aatgactgat
     7501 ataatcgtta tactttacac agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa
     7561 atgccagatg ggagcgaaat ttactatgaa aagagcggtc aaggcttccc tctcttttta
     7621 ctgcatggca atgacggcag cggccgcttt ttttcagaac aggtccctgt actcgaacgc
     7681 tactataccg tgtatctagt agatagccga ggacatggtc gttcgacaaa cgaagcatct
     7741 atgttgaatt ttcagttaat ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc
     7801 gatcaggcag attttcttgg atttagtgat ggagcaaatc tagcactggt atttgccagc
     7861 agcttcccta aaaaagttca tcgattgatt ttaaattctg gaaacacgct tgttaaaggc
     7921 gttcgttttt ctgccagagt catcagtaat attcattatg catgggtatg gttactctct
     7981 ttatttcgcc caagtcttcg caaaaatctt ttagttatca aacttttgct gcatgacatc
     8041 gggcttacag aaaatgattt aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa
     8101 aaagatgtaa tcaagctaaa acattctttg tatatagcta aaactatacc aaaagcttcc
     8161 tttgtgcttg tgaaagaaca aggacatgaa ttagctagaa aagatcctga acgcttcaat
     8221 cgagaagttc ttcaatttct ttctgaaaca taactattag gaatagaagg tgatttattt
     8281 gttaaaaaat accatacaat ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt
     8341 gatttctgtc attgtcatta ttgtcggtga attgatgtcc atcggtaaaa ctctatctat
     8401 ccagcaatta ggagaaacgt ttgccactat acctgtatgg aaaagcggac ttatgctgtt
     8461 gattggtctt gtctctgtgt tacctatgat tggttatgat attattttga atcggatgct
     8521 tgaacaaaag cagaatcctc gttacttgtt tgagacaagc tggctgatca atacaatcaa
     8581 caatattgcc ggtttcggcg ggtttgttag tgtggggctt cgctctgaac tatatggaca
     8641 aaagaaagag agcagaaacg tgatccaagc attatcgaaa gttttcttgt ttttgatggc
     8701 tggactttct atttacagtc tgctttcttt tttacttgta gtcttcacgc cagttgctcc
     8761 ttttttgaag caatattgga tatggttgat cggcggcggt ctatattttc ctgtcgtctt
     8821 tttatttact actttcaaaa agaaaggatt ccttggtggt acctcactta aaactcaagg
     8881 acagctattg ctagtttcat tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt
     8941 tggttattta atggaaatcc ggattgatct gtggcaaaca attccgttat tcattgctgc
     9001 ttcagtgata ggaatcgtgt ctatgattcc aggagaaatt gggagtttcg atgtaatgat
     9061 gattatcggg ttgtccgcta ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct
     9121 ctatcgcctg ttctactaca tcattccgtt tttgattgga atcgtctttt tcttcaaaaa
     9181 tatcggttcc acttttgatc agcgttattc tggtatccct aaacaattag caacagaaat
     9241 tgcgcataaa atcgtggttg tcttattata cttttctgga atcatgctag tactttctgc
     9301 cactatcccg caagctttta cagagtttcg ctggcttcat agtttgaacc cattgaaatt
     9361 tcattttatt atccaatttc ctagtatact attgggattt cttttaatca ttatgggcag
     9421 agggatcgca gcacgtgtga aacgagcata tttgcctaca atcttcttga ttgttctagc
     9481 tttactttat gttttattaa gtgattttag tttcacaccg gtggttttct tgtctattct
     9541 gcttctgatc attcttgctt ctaaaaacga attatttcgg gaacaattga tctattcatg
     9601 ggagtggcgt acgatcgatg gaatcattat tggtgcactg tccttgcttt acatcgttat
     9661 tggggtatac aatctgccgg atttccctca tcggcgtcac cattttattt cctttttctt
     9721 atttccttct gaaaagattt ggttttcggg attgttggcc atcatcgctg ttagtttcat
     9781 gatcgttcta tttgttcatt tccttcaagg agaaaagaaa cagattggcg aagcctttaa
     9841 tgaagaaaag gccctgaaga tccttacaac ttatggagga aactccgata gtcaattgat
     9901 cttcttaaaa gataagcgga tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct
     9961 ccaattcgct tgcttcaata ataaatgtat tgtaatgggt gatccttctg gtaagaaaga
    10021 agattttcca gaagcaatcg aagcttttat cgaagaaaca gatcgactat gctatttacc
    10081 agttttctac gaaacaagcg aagagattgt tatgattttg catgaattcg gctacgattt
    10141 cattaagatg ggggaagaag cttatgtaga cctgaatagt tttacaactt cagggaaaaa
    10201 aatgaagggc actcgtgctg tcttgaatcg gatcgaacga gaaggattta catttgatgt
    10261 cttacaacca ccattttcta ctgagcaaat gagtactttc aaaaatatct ctgataattg
    10321 gttgggttca cgaaaggaaa aagggttttc tttaggcttt ttctctgaag attatctaca
    10381 aagagcgcct attgcagttg tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat
    10441 catgcctact tacacgaaca ataaagtagg aacgattgat ctgatgcgat atgatcctaa
    10501 aaaagcacct tctggcagta tggatttctt gttcttgcat ttgtttgcct atatgaaaga
    10561 agaacatatc caatggttca atttagggat ggcccctctt tcaaatgtcg gaacttccag
    10621 aaagagtttt ttacaggaaa ggattgcata cttggtttac gagtttggtt ctcattttta
    10681 ttctttccat ggattaagag aatataaaag caagtacgct acaaactggg tctcacgtta
    10741 tactctatat tctagagata gttggattgc atatgttatg attgctttgc tgattattga
    10801 taatgcgcca gttgagcaga cacaaggtag aataagtggt ttgaaaaaat ggatacgaag
    10861 aaagtattaa aaactgcata ccgatagatg aacactctca tgatgaataa aagagactga
    10921 agtaaaacga aagttcatgc ttctacaatc tcttcttata caattgaaga acaaagagcg
    10981 gtccaaacag aaatcctgtt tggaccgctc ttttatttta aacatttttc attaccaaaa
    11041 tattcgagtc gcgtttctta tgcttcgatc tctgtaacga tacctgaacc cactgtccgt
    11101 ccgccttcac gaattgagaa tgtcgttccg ttttcaacag cgattggatg gatcaagtca
    11161 acatcaatcg tcacgttgtc accaggcatg accatttctg tatcttctgg caatgtgatt
    11221 gttcctgtaa catcagttgt gcggaagtag aattgtggac gatagttgtt gaagaatgga
    11281 gtatgacgtc caccttcttc ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt
    11341 ggagtaatgg aacctggttt agcaagcact tgtccacgtt cgatatcgtc acgttggatc
    11401 ccccgtaaca acacgcctac gttatcccca gcttccccgt aatctaacgt tttacggaac
    11461 atttctacac ctgttacaac tgctttttgt gtttcaggtt tgatccctac gatctctacc
    11521 tcatcaccga cacgaacagc accacgatca atacgacctg aggcaacagt tcctcgacct
    11581 gtaattgaga agacgtcttc cactggcaat agtaatggtt tatctgtatc acgttctggt
    11641 gttgggatat attcatctac tgtatccatc aattccataa tagcagcttc agcatctgga
    11701 tcgccttgca atgcttttaa tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa
    11761 ccatattcgc tcaataactc gcgaacttcc atttctacca aatcgatcaa ttcttcatca
    11821 tcgactaaat caactttgtt caagaaaaca atcaggtatt ttacaccaac ttggcgtgac
    11881 aataaaatgt gttcacgtgt ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt
    11941 gcaccatcca tttgtgctgc ccctgtaatc atgtttttca catagtccgc gtgtcctggt
    12001 gcatcaatat gcgcgtagtg gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc
    12061 gtaatcccac gttcacgttc ttctggagct gcatcaatac tagcatagtc ttgaggattt
    12121 gctaggcctt ttttgcctaa tactgtagtg atcgcagcag ttaaagtagt tttcccatgg
    12181 tcaacgtgtc caatcgtacc aatgttaacg tgtggtttac ttctgtcata atgttctttt
    12241 gccataattt acaaacctcc taaataatat agatcactga aaaatttcag ctctcacctt
    12301 tttattatag tctttttttg actaaatcaa aaagaatacg tccgacttac tttttgtaag
    12361 attcgaacta agcttattat acgtcaatat tggtcaaagt caactaagag cactcgcttg
    12421 tcttatacgc gaaaaaagta ccttttataa ataaaaaaat gtgttttttg agaaaaactt
    12481 caagatagtc cttaactttc ttttgaagca ataagaacaa aatgaggaaa agtttaaaat
    12541 tcagctgttg ccaacaactt tcaatagacg aaatcttcta tttcgttaat aataaggaaa
    12601 gaggtgaagt acatgaaaaa attaattggc gtacttgtcg ttttaggcat atttctcggc
    12661 ggaagttttg cagcttatca ttatttttac ggcggcgaag cttattatac aaaaatcacg
    12721 acgaatggcg aaatgtcagc tacccaagca gataacggag agaaattcac gacttatacc
    12781 tacaagcaag cggcttataa taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa
    12841 agagaacatc cattaaagat gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt
    12901 gtattaagct gggaagaaat aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc
    12961 gataattagt aaatggtctt aaaattttat taga
//
LOCUS       DBNIBO010000079        12957 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.083, whole genome shotgun sequence.
ACCESSION   DBNIBO010000079 DBNIBO010000000
VERSION     DBNIBO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.083"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..912)
                     /locus_tag="AC1Y77_001751"
     CDS             complement(<1..912)
                     /locus_tag="AC1Y77_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIQ1575323.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="AC1Y77_001752"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="AC1Y77_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIQ1575324.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2004..2174
                     /locus_tag="AC1Y77_001753"
     CDS             2004..2174
                     /locus_tag="AC1Y77_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575325.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="AC1Y77_001754"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="AC1Y77_001754"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIQ1575326.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="AC1Y77_001755"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="AC1Y77_001755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIQ1575327.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGNRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /gene="dapA"
                     /locus_tag="AC1Y77_001756"
     CDS             4438..5319
                     /gene="dapA"
                     /locus_tag="AC1Y77_001756"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIQ1575328.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /gene="dapB"
                     /locus_tag="AC1Y77_001757"
     CDS             5316..6032
                     /gene="dapB"
                     /locus_tag="AC1Y77_001757"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIQ1575329.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="AC1Y77_001758"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="AC1Y77_001758"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HIQ1575330.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="AC1Y77_001759"
     CDS             6749..7750
                     /locus_tag="AC1Y77_001759"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIQ1575331.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7759..9108
                     /locus_tag="AC1Y77_001760"
     CDS             7759..9108
                     /locus_tag="AC1Y77_001760"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIQ1575332.1"
                     /translation="MKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGKRTSD
                     DDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQKQLED
                     EKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITKAFKR
                     YPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMADPRI
                     IDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGTKISN
                     HEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPSGIDN
                     IGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSGKVLS
                     ILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTPSNS"
     gene            9068..10219
                     /locus_tag="AC1Y77_001761"
     CDS             9068..10219
                     /locus_tag="AC1Y77_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIQ1575333.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="AC1Y77_001762"
     CDS             10194..11363
                     /locus_tag="AC1Y77_001762"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIQ1575334.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="AC1Y77_001763"
     CDS             11538..12917
                     /locus_tag="AC1Y77_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1575335.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4317 a         2259 c         2827 g         3554 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatttgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg caaccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgcc
//
LOCUS       DBNIBO010000080        12946 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.084, whole genome shotgun sequence.
ACCESSION   DBNIBO010000080 DBNIBO010000000
VERSION     DBNIBO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.084"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(77..149)
                     /locus_tag="AC1Y77_001764"
     tRNA            complement(77..149)
                     /locus_tag="AC1Y77_001764"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="AC1Y77_001765"
     CDS             530..1099
                     /locus_tag="AC1Y77_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIQ1575336.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="AC1Y77_001766"
     CDS             1086..1946
                     /locus_tag="AC1Y77_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1575337.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /gene="yycF"
                     /locus_tag="AC1Y77_001767"
     CDS             2136..2840
                     /gene="yycF"
                     /locus_tag="AC1Y77_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HIQ1575338.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="AC1Y77_001768"
                     /gene_synonym="yycG"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="AC1Y77_001768"
                     /gene_synonym="yycG"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HIQ1575339.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="AC1Y77_001769"
     CDS             4677..5993
                     /locus_tag="AC1Y77_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YycH family regulatory protein"
                     /protein_id="HIQ1575340.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="AC1Y77_001770"
     CDS             5994..6863
                     /locus_tag="AC1Y77_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HIQ1575341.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="AC1Y77_001771"
     CDS             6918..7727
                     /locus_tag="AC1Y77_001771"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1575342.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="AC1Y77_001772"
     CDS             complement(7830..8459)
                     /locus_tag="AC1Y77_001772"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIQ1575343.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="AC1Y77_001773"
     CDS             8629..9921
                     /locus_tag="AC1Y77_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1575344.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="AC1Y77_001774"
     CDS             complement(9997..10659)
                     /locus_tag="AC1Y77_001774"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIQ1575345.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="AC1Y77_001775"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="AC1Y77_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIQ1575346.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="AC1Y77_001776"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="AC1Y77_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIQ1575347.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4315 a         2238 c         2802 g         3591 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacg
//
LOCUS       DBNIBO010000081        12886 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.085, whole genome shotgun sequence.
ACCESSION   DBNIBO010000081 DBNIBO010000000
VERSION     DBNIBO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.085"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            200..634
                     /locus_tag="AC1Y77_001777"
     CDS             200..634
                     /locus_tag="AC1Y77_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1575348.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            735..1826
                     /locus_tag="AC1Y77_001778"
     CDS             735..1826
                     /locus_tag="AC1Y77_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HIQ1575349.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1856..2533
                     /locus_tag="AC1Y77_001779"
     CDS             1856..2533
                     /locus_tag="AC1Y77_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIQ1575350.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2865..4160
                     /locus_tag="AC1Y77_001780"
     CDS             2865..4160
                     /locus_tag="AC1Y77_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIQ1575351.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4150..5610
                     /locus_tag="AC1Y77_001781"
     CDS             4150..5610
                     /locus_tag="AC1Y77_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575352.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5746..7242
                     /gene="glpK"
                     /locus_tag="AC1Y77_001782"
     CDS             5746..7242
                     /gene="glpK"
                     /locus_tag="AC1Y77_001782"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIQ1575353.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7256..9079
                     /gene="glpO"
                     /locus_tag="AC1Y77_001783"
     CDS             7256..9079
                     /gene="glpO"
                     /locus_tag="AC1Y77_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HIQ1575354.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9080..9796
                     /locus_tag="AC1Y77_001784"
     CDS             9080..9796
                     /locus_tag="AC1Y77_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIQ1575355.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10102..11577
                     /locus_tag="AC1Y77_001785"
     CDS             10102..11577
                     /locus_tag="AC1Y77_001785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIQ1575356.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGRNRRKPSFVDCS"
     gene            11579..12031
                     /locus_tag="AC1Y77_001786"
     CDS             11579..12031
                     /locus_tag="AC1Y77_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIQ1575357.1"
                     /translation="MDMSMKLIHQAERYLAEKAYRTQKKEFLPKTAVTNRKENKKERQ
                     LFAKGDRIFVNEYQKEALVYEDIGEDTIDVYLDKKIIHVPRQRVRLVRSAEDLYPTGY
                     DLDSLFIDYKTRKRQRDLERGSKKAHKVLVKEMRKRQEERRVNDENSK"
     gene            12157..12711
                     /locus_tag="AC1Y77_001787"
     CDS             12157..12711
                     /locus_tag="AC1Y77_001787"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIQ1575358.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4317 a         2139 c         2917 g         3513 t
ORIGIN      
        1 atactcaaaa ctaaacgact ttttcacaac ctctgattca cggtgttcca gaaggagttt
       61 tgtcgaaaac aagccgaaat tctttgaaga tggctacttc tttttttcaa atctagacag
      121 aggaggcgga cattattccg tgttttttga caaaaaataa aaatcatact atgatatgta
      181 cgccaatgga ggggatgaaa tggaaaagcc aattgaaaaa gcgttagatg aatgtctttt
      241 ctttagcgtg aaaaagctag atcgcatgtt gaataaatta gctgatgaag cgtttagaag
      301 gacaggtctg gcaccaactt atggttttat tttattgata ttaaaagaaa aagatggaat
      361 accacaaaaa gatattgcac aaatgctcta ttccgcccca tcaacgattg ctcgttttgt
      421 tgaaaaatta gaatataaag gatacgtcaa aacagttagc gaaggtcgct tgtcattagt
      481 ttatcttaca gaacaaggac gccaatttgc taaggaagtc gatggtagct gggaagaact
      541 tcatcagtca tacaaagcag ttttgggaaa tgaagcaagt aaagaattag cccagacaat
      601 gaatgatgca acagataagt tacaaaaaaa ataaaatttt ttaagcaaat tagttgcatg
      661 tacaactaat cgagtggttt aatgatagat gattctcgat aggttaatca gagcgaatta
      721 tgggaggaat gacaatgtca caatttgatc aatcaattac tttaccttca ggagttcaat
      781 tgaagaaccg attgatgctg tcgcctatga cgacacaact cagctttttc aatggagtga
      841 tcaccgaaga tgagatcact tattataaac aacgttctaa aggtcttggt gctgttatta
      901 ctggtgctgc aaatgtccaa gatatcggaa aaggctggcc aggtgaactc agcatcgctc
      961 atgatgaaat gattcctcga ttaagtgagc tagcgaaagc aatccaagga gaaggcgcaa
     1021 aagcgattat tcagattttc cacggtggac gaatgacaag tcgagcaact ttatctggag
     1081 aacagcctgt atctgcaagt gcagttgcag caaaacgtcc tgatgcggaa acaccacgag
     1141 caatgagtga agaagaaatc gttgaaacaa tccaagcatt tggcaaagcc acacgtagag
     1201 cgattgaatc aggatttgat ggaatcgagt tgcatggagc aaatacgtat ttgatccaac
     1261 aatttttctc tccgcattcg aatagacgag aggatcaatg gggcggtagc ttggaaaatc
     1321 gttatcgttt tattgaagaa ttattaacaa cggttttctc agcggttgaa caatatgcta
     1381 aaaaaccatt catcgtaggg tatcgtttct caccagaaga atatgaaaca ccaggtatcc
     1441 gttttgaaga cacgatctgg cttcttgaac gattacgtga gtcaaaacta gattatctgc
     1501 atgtttcatt aaatatgtat gatcgaatag ctcgttctga taaatacaac gataaatcga
     1561 ttcttgaagt ggtccataac acgattcaag gaaagatccc tttagttggt gtcggtggga
     1621 tccgcaatcg cgaagatgta gagcaggtat tgaaatatgc agaacttgca gcaattggac
     1681 agcaaatgat cgtcgatcct gactgggcag agaaattatt ggaaaaccgt gaggatgaat
     1741 ttgtaacgaa accttttgaa gaagcatata aagagcttta tttgccaagc ccattatata
     1801 atttcttgaa agcacgttat caataacgca atttttatag aaagaggatg ggaaaatgaa
     1861 agtattgatc gtattaacaa atgtagaaaa gtacgaaaag atcgagcgtc ctacaggttt
     1921 gtggttaagt gaactgactc atttttatga tgtattagta aaaaatggga tcgatgcaga
     1981 ttttgtcagt cctaaaggcg gatatgtacc tttagaaccg caaagtgttt tgaatatgga
     2041 tgaaatcgat tgggcctatt atgaagatga agatttcaga aatcgggcct tgggaaatac
     2101 cttacgtcct gatgatgtgg atgcaaatga ttatgcagcg gtttattatg ctggtggcca
     2161 cggaacaatg tgggattttc cagaagcaat agatcttggg aaagtcgctt cacaaatcta
     2221 tcgaaatgaa gggctggtct cagctgtttg tcacggtgtt gtaggattgt tgccagttac
     2281 agatgagtct ggaaaatcta ttttagctgg acgtacagtg actgggttca gtaatgaaga
     2341 agaggaagca aatggtacaa cgaatgaagt accattctta gcagaagatg ctttgaaaga
     2401 aaaaggagca aactatcagt ctggagcagc tttttcagaa gtggttcaaa ttgatgggcg
     2461 gcttcttaca gggcaaaatc ctcaatctgc tcatcaatta ggcgaagcag ttgttgaagc
     2521 attaaagaat taaaaataaa tgatgtgtct attctaacta ttacattaca cacgctaaaa
     2581 ctcgtctttt cgttcttttc tgtttcctat ttggacagag gtacgtaaaa ggcgagtttt
     2641 tttcttttat aagagtacat tttttgaaac atctcttcag aaatgatact cttcaattag
     2701 gtccactagt acattattga gaagcagacc tagaacacgg tatgatggag agggttgatt
     2761 ctgctgcatc gctatgtatc ataaggttga aagcgagtta tgaatactgt gttttctgat
     2821 tgagaagtta gcagtagaac accttataag aaagaggaat gcagatgaac attgtaattg
     2881 tcggcgggtc gtttgcaggg attcatgcgg ctatttatct tcgccaattg atgccaacaa
     2941 gtgaaatata tttgattgaa aagcaatcga agttaggatg gattcccagc gggttcaatt
     3001 tgatattaaa aggaaaggtt accagtgaaa aacaactttc ttggatcacg aaagaagaac
     3061 tgactgaccg ctatcgaatc catgtttaca cagaaaagac agtaatagga ttgaaagaaa
     3121 aaaaagtcgt tttagcagga ggaaaaaaat tggattttga tcgtttgatt ctagcgactg
     3181 gttcaaatca aaagtttcga aatatttctg cagaatcaac gcttattcac ccagttaaaa
     3241 agatacaaaa cgtaacggct cttcagcaaa agatcatgaa tgcaaagaaa attgcaatta
     3301 ttggtgcagg acaagcagga atcgaaatag ctgaagggtt ggcatcaaga aaaaaacaaa
     3361 tccatcttta cgaaagccga aagtcaattc tttttcgtta tctagatcct gaaatgacag
     3421 aaccgttagt aaaggaaatg aaaaatcaag ggctttcact ttttttagaa gaacaagttg
     3481 tgtctttgac cgaaaatgaa accgctattg tagagacgga aaaaagaaag gagaactatg
     3541 atcttgtttt gctggctaat cattctcgtc ccgacaatca gatgtgggaa gaacaattaa
     3601 cattgaatga tgatggaacg atctgggtag atgactactt gcaaacgtct caaaaagacg
     3661 tttatgcgat tggcgatgcg atccaagtaa cttttcgtcc aacaaatgaa aaaatgtatg
     3721 tttctctagt aaataacgct gtccgtacag cacgaaatgt cagcaagaca attagtggct
     3781 cacagacaaa ggatcaggga acctatcgtc cagtcggcaa tcagtggttt ggctatttct
     3841 taggaagcgt aggtttgaca gaagaagaga gcatttttta tccccaaaaa attgaaacag
     3901 gatacgtaga ctacaagctt acagcaataa atcaaagcaa agttaaactc aaatttctat
     3961 ttgattctga aaaaaagatt cttggagcac agctccaaag caaaaaagag atgttccacc
     4021 ttcttgacca actgacaatt gcagtagaag aaggatggac gtttgaacag ttagaagaac
     4081 atgagctatt tttccagcca gaataccgtg tgccagatat ggtgtggtca aaggtggcga
     4141 atactgccta tgaagattga agcattatta gaaagaaaag agcagttaca gatcctgatt
     4201 ttgcgaaacc ttgttctgca agggggaaca gcctctatca atggtttgag agagcatgtc
     4261 caactgtcaa aagcctcgtt tgatcaatat ttggaagata ttgaattgat tggccgaatg
     4321 atggaaaaaa aagtagaagt acagcgaaat gagtatcagg cgctgcttgt gttggacgaa
     4381 gatgtaagtt tggaaaaaat tctattgttt ttattgcagg aaagcttgaa gtttaagatg
     4441 ctcgtctatt tattagaaca tcagcaagta tccatcgttc gattagcgac agcgtttaat
     4501 atcagtgagt cttctgtttt tcgaaagatc aaagaattga accagctctt agaggaattt
     4561 ggattacaaa tcaaaaacgg tcaattatac ggagaagagc tacagatacg atatttttat
     4621 tatgaactat tccaatatat cccggaagat cagcgaccgc tttttttgca aaatacacca
     4681 gaaaaaagac cgttcattct aggattggac cgtgtattag aaacaacgtt tacggcatca
     4741 gcagaagcac agattgcttg ctggctcggg attacgaaaa aaagactatt gaatgaaaaa
     4801 agtacgtacg caactttgaa agaaaagaaa ttactctatc agtcggaccg gctttatcaa
     4861 gcaatcgatc caatcatcgt gatgcatctt agtcgaacag cagcagaact gaatgtatac
     4921 gaaacaatga tgttttacag cttttttgtt tctttttcga tagtagacga agaaatattt
     4981 tatcagtatg atttgacacg gagcaaaaag ttaccaaccg ctgttttaga tacttacatt
     5041 cgggaaacga tgttatggca ttatcgtcct cgtcgtttga aaatcaaaga agaaaaggca
     5101 gtaggctacc aaatttccca gatcaacaat gaatggtttt tctttgttgg aaagatcgaa
     5161 gtctatgagc gtgatcggct tttggaacag cagcagaaaa tgctcgggca ttccttaaca
     5221 cagttactgg cgaaattaca ggaaacagcg gtgcagcaat tgccgaggaa aagagctgaa
     5281 gatagtgagc ttagttacct gatgatccag tatgccaatg ttcttttgat gatcgatttt
     5341 tatattgcaa aaccagtagt gattggaatc gatttagaaa gtctgccgat ttatcgtatt
     5401 gcttttcaac aatatctgat cagagaactg aggggaatcg gcggtattga gattggaagt
     5461 tatgaagaag gtaaagaata tgatttggtg atcacatttt gccagagaaa caaaaaacag
     5521 tgtgaatatt atctgtcaga atttgcgtca ccttacgaca ttattcgatt gaaaagaaga
     5581 atagagacgc tgaaaaaaga aaagaattga ttaaaaaata gcttcataat aaaaaactgt
     5641 caaaaaaaga aaaaaagaat aaaattttga cagtttcttt ttttaaaaca gtagtatgat
     5701 aagagtagtt aaagaaagcg tttgcatata atggagggaa tcacaatgac agaatcaacg
     5761 tatatcatgg cgattgacca aggtacaact agctcacgag caattatctt tgacaagaaa
     5821 ggaagacaca tcggcagttc tcaaaaagag ttcacgcaat atttcccgca agaaagttgg
     5881 gtggaacatg atgcaaatga aatctggaat tctgtacagt cagttattgc cggagcgttc
     5941 atcgaatcag ggatcaaacc aaatcagatt gctgggattg gcataacgaa ccagcgtgaa
     6001 acaacagtaa tatgggaaaa agatacagga cgtccaatct atcatgcagt tgtttggcaa
     6061 tccagacagt cagctggaat tgcagataac ttaaaaaatg aaggctatca agaatttttc
     6121 cacgaaaaaa cagggttagt gatcgatgcc tatttctcag caacgaagat acgctggatt
     6181 cttgatcatg tagaaggcgc gcaagaacga gctgaaaaag gcgaattgat gtttggaacg
     6241 atcgattctt ggttagtatg gaaactgacc gatggacaag cacatgttac tgattactcg
     6301 aatgccagtc ggacgatgtt gttcaatatc catcagctag attgggatca ggagatttta
     6361 gatttactaa atattcctcg agtgatgtta ccaaaagtca cttctaactc agaagtttat
     6421 ggttatacgc aaggttatca tttttatgga agcgaagcac caatttcagg gatggcagga
     6481 gaccagcaag ccgcattatt cggacaaatg gcattcgaac caggcatggt caagaatact
     6541 tatggtacag gttcatttat tgtcatgaat accggtgaaa aaccgcaatt atccaaaaat
     6601 aatttattga cgacaattgg ttacggcata aatggaaagg tttactatgc tttagaagga
     6661 agtatttttg tggctggttc ttcgattcaa tggctgagag atggacttca aatgttgcaa
     6721 aaagctagcg attcagaaga cgcagcgaaa cgctctacaa gcgaagatga ggtttatgtt
     6781 gttcctgcat tcgttgggct aggtgctcct tattgggatc aggcagcacg aggggctatg
     6841 tttggcttga cacgtggtac gacaaaagaa gatatcatta aagctacttt acagtcgatt
     6901 gcttatcaag tgagagatat catcgatacg atgcaagatg acactggaat caagattcct
     6961 gtattgaaag tagatggcgg tgcagcaaac aatgaatatc tgatgcaatt ccaaacagat
     7021 atcttgaatg taccgatcca acgagcagaa aacttggaaa caacggcgct aggggcagca
     7081 tttttagcag gattagctgt cggttattgg aaagatacag acgagatccg cgaattttat
     7141 gaagctggca agctatttga agttcaaatg gaggaagagc gccgagaaaa actttacaac
     7201 ggttggaaaa aagcagtaaa agctacccaa gcatttgaat aaggaggaaa tccatatgtt
     7261 ttcaaacaag acaagacaag atagcattca aaaaatgcag caagaagaat tggatctgtt
     7321 gatcatcggt ggcggaatca ctggtgccgg tgtggcagtc cagtcagcag catcaggaat
     7381 caaaacagga ttgatcgaaa tgcaggattt tgcagaaggg acttcctctc gctcgaccaa
     7441 acttgttcat ggcggtattc gttatctgaa gacatttgat gtggaagtag tagctgacac
     7501 agttggtgaa cgtgcagtcg tacaaagtat tgccccacac atcccaaaac cagatccaat
     7561 gcttttgcca atctatgagg gcgaaggagc aacaaccttc aatatgttct cggtcaaagt
     7621 agcaatggac ctttatgaca aactggcaaa tgtgacagga accaaatatg agaattatac
     7681 gctgacacca gaagaagtat tggaaagaga accgtttttg aaaaaagaag ggctaaaagg
     7741 cgcgggtgtc tatctagatt tccgcaacaa tgatgcccgt ttagtgattg ataatatcaa
     7801 aaaggctgcc gaagatgggg cttatctagt aagtaaaatg aaagcggttg gctttttata
     7861 tgatggcgat caaatcgttg gcgtcaaagc tcgtgacctg ctgacagatg aagtgatcga
     7921 aatcaaagca aaattagtga tcaatacgag tggtccttgg gtagataaag tacgaaactt
     7981 gaattttacg cgtccagtct ctcctaaaat gcgtccaact aaagggatcc atttagttgt
     8041 agatgcgaaa aaactgcctg taccgcaacc cacatacttc gatacaggaa agcaagatgg
     8101 gcggatggtt tttgctattc caagagaaaa caagacttac tttggtacga cagatacgga
     8161 ttaccaagga gattttacgg atccgaaagt cacacaagaa gatgtagatt atctattgga
     8221 tgtgatcaat catcgctatc cagaagcaaa tatcacattg aatgatatcg aagcaagctg
     8281 ggcaggactt cgtccactgt tgattggtaa ttctggttct gattacaatg gtggagataa
     8341 tggatcgatt tcagacaaga gcttcaataa agttgtcgat acagtaagtg aatataagga
     8401 aaacaaagtt tctcgtgctg aagtagaaga cgtgttgaac catttggaaa acagccgtga
     8461 tgaaaaagca ccttctacga tttccagagg tagttcttta gaaagagaac cagatggttt
     8521 gctgacttta tctggtggga aaatcaccga ttaccgtaag atggcagaag gagctttgcg
     8581 attgatacgc caattattaa aagaagaata cggaatagag acgaaagaaa tcgactctaa
     8641 aaaatatcag atttcaggtg gaaacttcga tccaactaaa ttagaagaaa cagtgacaga
     8701 attagcaaaa gaaggagtag cagccggttt agaggaagaa gatgctactt atatcgctga
     8761 tttttacggg actaatgccc gacgtatctt tgaattagca aaagaaatgg caccttatcc
     8821 tggtttgagt ctcgctgagt cagctcggtt acgttatggt ttagaagaag aaatggtttt
     8881 ggccccaggc gattatctca ttcgtcgtac aaatcatctg ttgtttgaac gagatcagct
     8941 tgatgagatc aagcaacctg tgatcgatgc aattgctgga tattttgaat ggacagaaga
     9001 ggagaaggcg caacagacta aacgtttaga agcattgatt gcagaatcag atctgcggga
     9061 actaaagggg gagaaataaa tgaacagtga tatgactcaa atcatggggg agtttattgg
     9121 gacactgatc ctagttttgc ttggagatgg tgtgtgtgcg gctgtgaatt taaacaaaag
     9181 taaagcacag gcttctggct ggattgtcat cgcatttggt tggggactcg ctgtaacaat
     9241 ggctgtctat atttctggat tcatgggccc agctcatttg aatcctgcag tctctctagc
     9301 tatggcgatg acaggggcaa ttagttggaa tcttgtcgtt cctttcatca ttgcgcaagt
     9361 tttaggagct tttgcagggg ccattcttgt ttggctttct tatttgccac attggaatgc
     9421 aacgaaagat gaaagcgcga ttttaggaac gtttgcaaca ggtcctgcta tacggaatta
     9481 tccagcaaat gtgatcacag aattaattgg tacatttgta ttagttcttg ggttgctagc
     9541 ttttggacaa aacgaatttg cacctggaac gaacgtattc gctgtaggtg gattgatctt
     9601 agccatcggt ctttcattag gagggccaac aggatatgca atcaaccctg ctcgggactt
     9661 tggtcctcga ttagcacatg cggtattgcc aatagctaat aaaggaactt cagattgggc
     9721 atattcatgg gtaccaatcg caggtccaat gatcggagca attatcgcgg taggtgtcta
     9781 ttcattaatg gtgtaaatct ataaaacata ctaaagtttg acaaccaaaa tgtgtcttgc
     9841 tatagttaag tggaaaataa ataagaccta gaagaatggg gtatgttttg tgagtgtagc
     9901 tggtatctaa tcagtaagtc gaatagccaa taaacaaagt ttatttggtg tgcttatgaa
     9961 acggtacctg tcaggaaaac aatatctttg tattctaata aggataggac atgacttttt
    10021 taagctgtgt tctatccttt tatttatctc taggccctta tttattttgg gacaggtatc
    10081 tcttcaagaa aaggagagaa tatggacaac gaaatgatta gtcaattaca atttgatcgg
    10141 atcaaaaagg aaatccaagc aagagcaatt ggaaactata gcaaaaaacg aatcagcgat
    10201 atgacggtct cgacaaacct acaaactgtc ttggatcgtc aagaagaaac aagagaagca
    10261 cgcttgatct tagaaagcag tcagcatgtt ccatttatgg ggttgccgag gatcgatgca
    10321 ctgacagaac aagtcaaaaa agggttgatt ctgcagccaa ccgatttgat cgaatatgcg
    10381 gattttctta gaagcagtcg gatgatcaca aagttttttg ataagaatca ataccagaca
    10441 cccttgttgt ttgcttatag caaacatctt cctgatttga taaatgtaga agagttgatt
    10501 gaccagaaaa taaaaaataa taaagtcagt gacgacgcgt cacgtaattt gcgtaaagta
    10561 cgcaaacaat tacaactaat cgaaaaagaa atccagtcaa aattactgaa attcttgcgt
    10621 catccgaaaa acaaagaaat gatccaagaa gcaatgattg ttcaaaaagg cgaatactat
    10681 acgattccta tcaaagccag ctataaaaat aaaatagatg gaacgattat cgatgaatca
    10741 aataaaagaa cgacggtttt tattgaacca actgtgattt ctaaattgaa tgaacactat
    10801 cagttgttaa aagctgaaga aatcagtgaa gaatatcaag tcctagcggc attgactggt
    10861 gctatcgcag aaaacgaaga agtgatcgat ttattgatcg aaacgatcac ggtgttagat
    10921 atcatctttg ctcgggcaaa atttagccga gaaatcaatg gaagcacacc tagaatcaat
    10981 aaatcggaac atatcattat caagcaaggc agacatccat ttttacctga gcatgcggta
    11041 ccattggatg ttgaaatcgg taaagactat cgaggcttga ttatcaccgg agcaaatgcc
    11101 ggaggcaaaa cagtcgtgtt gaagacagtc ggtttattga ctttgatggc gatgttcggt
    11161 atgcaagttc cggcaaaaga agggacagag cttgctgtct ttgatgagat tttcgtcgat
    11221 gtcggtgacc accaaaattt agaaaatgcg ttgagtacat tttctggtca tatgcaaaac
    11281 atcgctgcta ttttgaaaaa gataaaaagg aatacgttgg tacttttaga tgagattggc
    11341 agtggaacag agccaaatga aggggctgca ctagcgatcg caatcatgga atctatgtat
    11401 gagcaagggg cattgatcat tgccacgacc cattatggag aaatcaagaa attcgccgga
    11461 gatcatgaag attttgtgcc ggctgctatg gctttcgatc gggaagcact tcgtcctaaa
    11521 tatcagttgc gggttggaag aaacaggaga aagccaagct ttgtggattg ctcataaaat
    11581 ggatatgtcg atgaaattga tccatcaagc agagcgttac ttggcggaaa aagcctatcg
    11641 gacacagaaa aaagaatttc ttcctaagac agcagttaca aataggaagg agaataaaaa
    11701 agaacgtcag ctctttgcaa aaggtgatcg gatatttgtc aatgaatacc aaaaagaagc
    11761 gttagtttat gaagatatag gtgaagatac gattgatgta tatttagaca aaaaaataat
    11821 ccacgtgcct cgtcagcgtg ttcgtctggt gcgttcagca gaagatctgt acccgacagg
    11881 ttacgattta gacagtctgt ttatcgatta taaaacacgt aaacggcagc gtgacctaga
    11941 gcgaggatca aaaaaagctc ataaagtact agtaaaagaa atgcgcaaac gacaggaaga
    12001 aagaagagta aacgatgaaa acagcaagtg attatctcaa attagctcat aatatcagtg
    12061 tctgtaaaga aaaataaaaa cagtgaaatc attggtttta ttccccgtgt aagctaaaat
    12121 aaaaagaaaa gagtgctgag gaggtagggc gataaaatga aattaagcac agactggcgt
    12181 aaagaaatac aaacgattcc gaatctttta tcgatattcc gtattttttt gctgccgatt
    12241 tatctttatt ttgtgctgag acagtcattt tatatagcag gagcggtcat tgttgtctca
    12301 ggtttgtctg attatctaga cggggtcatt gctcggagat ataatcaggt aacagactta
    12361 gggaaggtgc ttgatccgtt tgcagataaa ttgacacaac tttttctgat tttgtctatg
    12421 gcatggtatc gaccgtggct ttggctgttg tttggtctat tcttgataaa agaaggcttt
    12481 atgtttgtag ctggattaat cgggctatct aaaaatatca aattaagtgg agcgaagtgg
    12541 tatggcaaag tagcgactgc tgtcatctat gttggcatga tccttttgct gcttttccca
    12601 gaattaccaa cgctgtgggt gagagtcatt tttgccgtca tcacttacgg tcttttacaa
    12661 tcttttgtct tgtatgctgt cgagtaccga aagatgtttc aaagaaaata aaaaaggggt
    12721 gtgacctatg tcgcattcct ttttttgata aatagactcc cagtagctac ttctcgataa
    12781 taagccaacc gaaccaaaaa caggagaagt tgtttttgaa attatttctt gtgattcgga
    12841 gttaaatggt aggctcacaa cctcttggta aacggtgttc aatcag
//
LOCUS       DBNIBO010000082        12831 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.086, whole genome shotgun sequence.
ACCESSION   DBNIBO010000082 DBNIBO010000000
VERSION     DBNIBO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.086"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            85..624
                     /locus_tag="AC1Y77_001788"
     CDS             85..624
                     /locus_tag="AC1Y77_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575359.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="AC1Y77_001789"
     CDS             1644..2813
                     /locus_tag="AC1Y77_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIQ1575360.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="AC1Y77_001790"
     CDS             3286..3840
                     /locus_tag="AC1Y77_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1575361.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4495)
                     /locus_tag="AC1Y77_001791"
                     /pseudo
     CDS             complement(4013..4495)
                     /locus_tag="AC1Y77_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4498..5445
                     /locus_tag="AC1Y77_001792"
                     /pseudo
     CDS             4498..5445
                     /locus_tag="AC1Y77_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5716..6163
                     /locus_tag="AC1Y77_001793"
                     /pseudo
     CDS             5716..6163
                     /locus_tag="AC1Y77_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            6167..7000
                     /locus_tag="AC1Y77_001794"
     CDS             6167..7000
                     /locus_tag="AC1Y77_001794"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1575362.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7245..8270
                     /locus_tag="AC1Y77_001795"
     CDS             7245..8270
                     /locus_tag="AC1Y77_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1575363.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8408..8533
                     /locus_tag="AC1Y77_001796"
     CDS             8408..8533
                     /locus_tag="AC1Y77_001796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575364.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8558..9673
                     /locus_tag="AC1Y77_001797"
                     /pseudo
     CDS             8558..9673
                     /locus_tag="AC1Y77_001797"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            9756..10652
                     /locus_tag="AC1Y77_001798"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="AC1Y77_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10668..11492
                     /locus_tag="AC1Y77_001799"
     CDS             10668..11492
                     /locus_tag="AC1Y77_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575365.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..>12831
                     /locus_tag="AC1Y77_001800"
     CDS             11524..>12831
                     /locus_tag="AC1Y77_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HIQ1575366.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
BASE COUNT         4473 a         1860 c         2364 g         4134 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa t
//
LOCUS       DBNIBO010000083        12825 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.087, whole genome shotgun sequence.
ACCESSION   DBNIBO010000083 DBNIBO010000000
VERSION     DBNIBO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.087"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(83..802)
                     /locus_tag="AC1Y77_001801"
     CDS             complement(83..802)
                     /locus_tag="AC1Y77_001801"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIQ1575367.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(851..2497)
                     /locus_tag="AC1Y77_001802"
     CDS             complement(851..2497)
                     /locus_tag="AC1Y77_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIQ1575368.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2676..3266
                     /locus_tag="AC1Y77_001803"
     CDS             2676..3266
                     /locus_tag="AC1Y77_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIQ1575369.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3358..4863
                     /locus_tag="AC1Y77_001804"
     CDS             3358..4863
                     /locus_tag="AC1Y77_001804"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HIQ1575370.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(4947..6299)
                     /gene="celB"
                     /locus_tag="AC1Y77_001805"
     CDS             complement(4947..6299)
                     /gene="celB"
                     /locus_tag="AC1Y77_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HIQ1575371.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6299..6817)
                     /locus_tag="AC1Y77_001806"
     CDS             complement(6299..6817)
                     /locus_tag="AC1Y77_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575372.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6833..7159)
                     /locus_tag="AC1Y77_001807"
     CDS             complement(6833..7159)
                     /locus_tag="AC1Y77_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HIQ1575373.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7172..9151)
                     /locus_tag="AC1Y77_001808"
     CDS             complement(7172..9151)
                     /locus_tag="AC1Y77_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1575374.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9172..9486)
                     /locus_tag="AC1Y77_001809"
     CDS             complement(9172..9486)
                     /locus_tag="AC1Y77_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HIQ1575375.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9654..9947
                     /locus_tag="AC1Y77_001810"
     CDS             9654..9947
                     /locus_tag="AC1Y77_001810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIQ1575376.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10025..11137)
                     /locus_tag="AC1Y77_001811"
     CDS             complement(10025..11137)
                     /locus_tag="AC1Y77_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIQ1575377.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11290..12138
                     /locus_tag="AC1Y77_001812"
                     /pseudo
     CDS             11290..12138
                     /locus_tag="AC1Y77_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12491..>12825)
                     /locus_tag="AC1Y77_001813"
     CDS             complement(12491..>12825)
                     /locus_tag="AC1Y77_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIQ1575378.1"
                     /translation="LTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKE
                     QEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISI
                     KFRKKRNI"
BASE COUNT         4038 a         2583 c         2172 g         4032 t
ORIGIN      
        1 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
       61 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
      121 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
      181 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
      241 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
      301 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
      361 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
      421 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
      481 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
      541 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
      601 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
      661 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
      721 cgatcgttcc ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg
      781 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
      841 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
      901 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
      961 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
     1021 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
     1081 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
     1141 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
     1201 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
     1261 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
     1321 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
     1381 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
     1441 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
     1501 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
     1561 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
     1621 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
     1681 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
     1741 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
     1801 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
     1861 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
     1921 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
     1981 tctgcgacag cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac
     2041 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
     2101 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
     2161 acaggcctaa cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
     2221 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
     2281 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
     2341 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
     2401 ttgaaatgat attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg
     2461 ttaattgatc taatgatggt tttctttgat tatccatcgg tcagattcca actttctaca
     2521 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
     2581 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
     2641 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
     2701 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
     2761 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
     2821 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
     2881 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
     2941 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
     3001 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
     3061 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
     3121 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
     3181 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
     3241 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
     3301 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
     3361 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
     3421 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
     3481 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
     3541 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
     3601 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
     3661 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
     3721 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
     3781 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
     3841 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
     3901 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
     3961 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
     4021 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
     4081 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
     4141 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
     4201 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
     4261 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
     4321 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
     4381 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
     4441 gactatgcac ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat
     4501 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
     4561 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
     4621 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
     4681 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
     4741 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
     4801 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
     4861 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
     4921 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
     4981 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
     5041 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
     5101 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
     5161 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
     5221 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
     5281 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
     5341 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
     5401 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
     5461 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
     5521 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
     5581 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
     5641 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
     5701 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
     5761 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
     5821 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
     5881 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
     5941 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
     6001 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
     6061 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
     6121 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
     6181 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
     6241 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
     6301 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
     6361 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
     6421 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
     6481 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
     6541 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
     6601 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
     6661 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
     6721 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
     6781 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
     6841 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
     6901 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
     6961 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
     7021 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
     7081 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
     7141 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
     7201 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
     7261 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
     7321 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
     7381 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
     7441 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
     7501 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
     7561 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
     7621 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
     7681 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
     7741 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
     7801 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
     7861 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
     7921 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
     7981 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
     8041 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
     8101 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
     8161 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
     8221 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
     8281 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
     8341 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
     8401 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
     8461 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
     8521 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
     8581 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
     8641 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
     8701 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
     8761 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
     8821 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
     8881 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
     8941 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
     9001 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
     9061 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
     9121 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
     9181 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
     9241 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
     9301 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
     9361 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
     9421 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
     9481 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
     9541 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
     9601 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
     9661 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
     9721 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
     9781 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
     9841 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
     9901 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
     9961 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    10021 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    10081 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    10141 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    10201 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    10261 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    10321 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    10381 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    10441 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    10501 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    10561 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    10621 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    10681 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    10741 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    10801 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    10861 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    10921 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    10981 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    11041 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    11101 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    11161 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    11221 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    11281 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    11341 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    11401 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    11461 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    11521 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    11581 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    11641 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    11701 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    11761 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    11821 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    11881 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    11941 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    12001 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    12061 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    12121 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    12181 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    12241 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    12301 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    12361 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    12421 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    12481 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    12541 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    12601 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    12661 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    12721 tcgcatccaa ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg
    12781 ctaagccatc tatcgccagt tttccatctg ctcctgtggt tagac
//
LOCUS       DBNIBO010000084        12726 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.088, whole genome shotgun sequence.
ACCESSION   DBNIBO010000084 DBNIBO010000000
VERSION     DBNIBO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.088"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            333..1961
                     /gene="treC"
                     /locus_tag="AC1Y77_001814"
     CDS             333..1961
                     /gene="treC"
                     /locus_tag="AC1Y77_001814"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HIQ1575379.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(2021..3295)
                     /locus_tag="AC1Y77_001815"
     CDS             complement(2021..3295)
                     /locus_tag="AC1Y77_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1575380.1"
                     /translation="MIDDIFAINLYHFPFIPAFVYNEVIDKGGKRVKKETDTTNRLIR
                     FLGGKTSYYVLGIVILLALIIYFFHQISFIFKPVGIIIATILPPLLFALILYYLLEPV
                     IKRLSKHLSRNLSVIIVYVIILFLLGLGSIWLIPFLEEQAKTLISSLPDLFQDFQESL
                     RQFLEKTPFADSFNQFFASIDDVTSDIAGFIGNYWESGAQRISNIFSTVTAIFITLFT
                     GPIVAFFLLRDPQKFYQSVLAIVPPAFRTDFKNLTKIANQQLGSFLKGQIIASFILGA
                     VYWVCFLLIGLEFASVLAISAGLLCIIPYIGPFIAFFPGLIIAFQDSTFMAVKFVIVW
                     FAVQLLHGDLVIPRVMGDRLQIHPITILIVLLVMGDLMGIVGVIFGIPIYTLVKLLVT
                     FIFRKFKQRYNKFYGDKGEYENSSFSEEDYFK"
     gene            3454..4977
                     /locus_tag="AC1Y77_001816"
     CDS             3454..4977
                     /locus_tag="AC1Y77_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HIQ1575381.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(5046..5774)
                     /locus_tag="AC1Y77_001817"
     CDS             complement(5046..5774)
                     /locus_tag="AC1Y77_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIQ1575382.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(5787..7067)
                     /locus_tag="AC1Y77_001818"
     CDS             complement(5787..7067)
                     /locus_tag="AC1Y77_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIQ1575383.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(7208..7810)
                     /locus_tag="AC1Y77_001819"
     CDS             complement(7208..7810)
                     /locus_tag="AC1Y77_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HIQ1575384.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            8055..8261
                     /locus_tag="AC1Y77_001820"
     CDS             8055..8261
                     /locus_tag="AC1Y77_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575385.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            8573..9292
                     /locus_tag="AC1Y77_001821"
     CDS             8573..9292
                     /locus_tag="AC1Y77_001821"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIQ1575386.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(9373..10056)
                     /locus_tag="AC1Y77_001822"
     CDS             complement(9373..10056)
                     /locus_tag="AC1Y77_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIQ1575387.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            10274..12460
                     /locus_tag="AC1Y77_001823"
     CDS             10274..12460
                     /locus_tag="AC1Y77_001823"
                     /EC_number="3.6.4.-"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIQ1575388.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         4603 a         2164 c         2440 g         3519 t
ORIGIN      
        1 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
       61 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
      121 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
      181 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
      241 caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag
      301 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
      361 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
      421 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
      481 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
      541 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
      601 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
      661 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
      721 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
      781 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
      841 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
      901 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
      961 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
     1021 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
     1081 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
     1141 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
     1201 aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg
     1261 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc
     1321 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
     1381 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
     1441 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
     1501 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
     1561 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
     1621 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
     1681 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
     1741 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
     1801 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
     1861 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
     1921 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
     1981 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
     2041 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
     2101 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
     2161 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
     2221 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
     2281 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
     2341 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
     2401 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
     2461 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
     2521 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
     2581 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
     2641 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
     2701 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
     2761 aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
     2821 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
     2881 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
     2941 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
     3001 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
     3061 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
     3121 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
     3181 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca ttatagacaa
     3241 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
     3301 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
     3361 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
     3421 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
     3481 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
     3541 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
     3601 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
     3661 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
     3721 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
     3781 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
     3841 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
     3901 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
     3961 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
     4021 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
     4081 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
     4141 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
     4201 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
     4261 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
     4321 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
     4381 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
     4441 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
     4501 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
     4561 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
     4621 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
     4681 aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
     4741 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
     4801 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
     4861 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
     4921 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
     4981 gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt
     5041 tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt
     5101 gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca
     5161 taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca
     5221 tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa
     5281 gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt
     5341 ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata
     5401 tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg
     5461 atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt
     5521 cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct
     5581 tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg
     5641 agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg
     5701 attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata
     5761 taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa
     5821 cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc
     5881 aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt
     5941 ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa
     6001 tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca
     6061 atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc
     6121 agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg
     6181 agttttaatg acagtaaact acggatctca ggaactttca tattatccaa catacttaga
     6241 ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac
     6301 cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg
     6361 taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc
     6421 tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc
     6481 caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc
     6541 aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat
     6601 gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa
     6661 gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca
     6721 atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa
     6781 tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg
     6841 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
     6901 gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa
     6961 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
     7021 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
     7081 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
     7141 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
     7201 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
     7261 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
     7321 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
     7381 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
     7441 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
     7501 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
     7561 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
     7621 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
     7681 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
     7741 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
     7801 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
     7861 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
     7921 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
     7981 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg
     8041 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
     8101 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
     8161 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
     8221 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
     8281 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
     8341 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
     8401 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
     8461 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
     8521 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
     8581 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
     8641 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
     8701 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
     8761 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
     8821 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
     8881 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
     8941 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
     9001 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
     9061 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
     9121 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
     9181 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
     9241 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
     9301 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
     9361 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
     9421 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
     9481 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
     9541 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
     9601 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
     9661 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
     9721 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
     9781 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
     9841 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
     9901 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
     9961 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
    10021 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
    10081 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact
    10141 aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata
    10201 gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa
    10261 ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg
    10321 ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac
    10381 ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga
    10441 aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa
    10501 tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg
    10561 agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa
    10621 acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact
    10681 aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca
    10741 tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca
    10801 aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca
    10861 gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc
    10921 tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa
    10981 agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg
    11041 attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt
    11101 actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa
    11161 atatgattat ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt
    11221 gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag
    11281 tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc
    11341 taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc
    11401 aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca
    11461 aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg
    11521 gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat
    11581 attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat
    11641 tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca
    11701 acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca
    11761 ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg
    11821 cgatcaatac ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat
    11881 aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa
    11941 aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat
    12001 agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc
    12061 tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa
    12121 aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca
    12181 caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt
    12241 gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc
    12301 agtaacgacg atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa
    12361 tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt
    12421 tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag
    12481 aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg
    12541 aaatttcagc ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag
    12601 gtgtgacaag aattttgtca cacctccctt atttattttg aaaatgagaa tactattatc
    12661 aaaatagaga atatatagaa aaattctcat tattcaaacg taaataatca gttattttga
    12721 tttttt
//
LOCUS       DBNIBO010000085        12284 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.089, whole genome shotgun sequence.
ACCESSION   DBNIBO010000085 DBNIBO010000000
VERSION     DBNIBO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.089"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..831)
                     /locus_tag="AC1Y77_001824"
     CDS             complement(<1..831)
                     /locus_tag="AC1Y77_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1575389.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(845..1642)
                     /locus_tag="AC1Y77_001825"
     CDS             complement(845..1642)
                     /locus_tag="AC1Y77_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIQ1575390.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1675..1959)
                     /locus_tag="AC1Y77_001826"
     CDS             complement(1675..1959)
                     /locus_tag="AC1Y77_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIQ1575391.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1956..2957)
                     /locus_tag="AC1Y77_001827"
     CDS             complement(1956..2957)
                     /locus_tag="AC1Y77_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIQ1575392.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2959..3861)
                     /gene="gnd"
                     /locus_tag="AC1Y77_001828"
     CDS             complement(2959..3861)
                     /gene="gnd"
                     /locus_tag="AC1Y77_001828"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIQ1575393.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4025..4873
                     /locus_tag="AC1Y77_001829"
     CDS             4025..4873
                     /locus_tag="AC1Y77_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1575394.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5519..5725
                     /locus_tag="AC1Y77_001830"
     CDS             5519..5725
                     /locus_tag="AC1Y77_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HIQ1575395.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5927..6928)
                     /locus_tag="AC1Y77_001831"
     CDS             complement(5927..6928)
                     /locus_tag="AC1Y77_001831"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1575396.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6933..8846)
                     /locus_tag="AC1Y77_001832"
     CDS             complement(6933..8846)
                     /locus_tag="AC1Y77_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1575397.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(9014..9520)
                     /locus_tag="AC1Y77_001833"
     CDS             complement(9014..9520)
                     /locus_tag="AC1Y77_001833"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIQ1575398.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9680..10120
                     /locus_tag="AC1Y77_001834"
     CDS             9680..10120
                     /locus_tag="AC1Y77_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1575399.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10146..11303)
                     /locus_tag="AC1Y77_001835"
     CDS             complement(10146..11303)
                     /locus_tag="AC1Y77_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575400.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(11306..11662)
                     /locus_tag="AC1Y77_001836"
     CDS             complement(11306..11662)
                     /locus_tag="AC1Y77_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575401.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(11960..>12284)
                     /gene="sgrA"
                     /locus_tag="AC1Y77_001837"
     CDS             complement(11960..>12284)
                     /gene="sgrA"
                     /locus_tag="AC1Y77_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HIQ1575402.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
BASE COUNT         3555 a         2513 c         1914 g         4302 t
ORIGIN      
        1 aaatccgtca tcatagtcat cttcgtcatc aaaagatgct gaattgttat tgcttgtggc
       61 aggctgtact tgtttccctt tttgcgactg gacaaagatc aaagcgataa taatcccaaa
      121 aatggcataa gtaaccatcg taatatctaa agatttcaaa acaacactca taaaataagc
      181 aaggaagaaa aagaccagat aatcttttcg accgataacg tatacagttg tcgcgatacc
      241 gattgccgct aaccctccac ctactacttc aagaatgtgg ataacgactg ttccctctaa
      301 agctttgatt acatcagcga tcaatggcgc ccctaaatat aaagcaatga aaacaagcgg
      361 aacgaataat aaaaatgagg caaccgttgg ataaagaata attgatcggg ccatagcttt
      421 tgtattcaaa tcttctacag ctttgtctgt atatttacct aaaaaagtat tgatgaaaaa
      481 acgaaactga tacaagtaac ttccgagcaa tccgactggc accgcaacgg caatcgctgc
      541 ttctggcgac agatccccta ataatgcgac tggtactgcg atagctgctg caatagatgg
      601 ttcagctggc attgatcctc ctggagaaaa aacccccata taaatcattt gcaaagcggc
      661 tgtaacaatc atcgcttgtg caacttcccc catgatcaac ccgacgacta atccagtcat
      721 caaaggagaa aaacgtaagg tcaacgtggc tcctccacct aaccagcggg acataaccgc
      781 ggctacccat aaagcaatca acaaactttg tactacactt aatgtttcca tgtgtcctcc
      841 tccttcagat ctttctgata taaccttcca gttccatcat tgactgtatc agtgatttct
      901 gtgttacctc tataggaatc aaatgcactt tctctttcga atcaataaat tcagccattt
      961 tatctattat ctgtttatct ttcggaaata atcccatttg ttctagagaa accggcagtt
     1021 ctaattcccg atacaaagga tataactcgt ctattttttc ccagcgacct tctagtgcca
     1081 gttgatagaa aatcccatac gctacttttt ctccatgtag aaaacgatgg ctttccggaa
     1141 tatatttact gatagcgtca tgcatagcat gtgctgccac atttcttgcg tatttatctc
     1201 ctaatcctcc aactaaacca gcaacagcaa agataatctc tgaaagctgt ttgaactccg
     1261 atgtcagttt tctttgtgcc atatcttgga ttgctttagc agaatgattc aaaatctctt
     1321 ctttgcagat cttcgcagta tcttgtgcta gcttcaaaaa tgattcatct tgcaaaaaat
     1381 cttgttctaa gatgagctca gattcatacc attttgctag tgtatctgct aacccggcaa
     1441 tgaaatatgg aacaggggaa tcaatcacta attggggatc tgttataaaa aaagcagctt
     1501 gtctgtaaaa gtgttctgtt ttcccttctg cttgtccatt ctccttatac atgacagcaa
     1561 gaggtgtcca tggagcacaa ttactagcta aagtagggat gactccgaaa tcgattcttg
     1621 caagattggc actataaccc actagatcgg tcagttttcc tccgccaacg ccaactacga
     1681 aatcgatttc atttttttga tgattgtagc tattcgtctg gcttcagcat aactacattc
     1741 tccagaataa cgatgataaa aaaattgaat cgtttcgtca ttcaaaaata ctaatttagg
     1801 tagcgccttt tcccatgata ccgttccgtg ggtgaccagt actcttttcg cgccgtatga
     1861 tttcaagaga tcagaaactt ttgaaatacc accttcttca tataaataaa attgagggcc
     1921 tgttttcact tttaaaccgt tcaccatttt cttcatcatc cttttatata gttgctcttt
     1981 gtctggacag aacgttcagg aagaaggtgc tgactgatct gttcgacatc tctgacacat
     2041 ttttcaccca ttgcttctaa acattccctt gtgtaggccg atatatgtgg cgtaatcact
     2101 acttgttcaa acgctaaata cggatgatct tgacgtcccg gttcttcttc taaaacatct
     2161 gtggcatatc ctgcgatctt tccgcttttc agaccattaa tcatcgcttc ttcatcaact
     2221 agagcgcctc ttgcactatt agatagataa acattttctt tcatctgatc gacttcttta
     2281 tcagaaatca tgtgatagtt ctcttctgtc aaattcgcac ataaacaaat gatatctgat
     2341 tgttccagta attcttgaag ttctactttt tttgcaccaa agctttcgat gtataaatct
     2401 gatttgtaag gatcataagc tagaacatca cagcgaaaac cgttacgcaa aatttcgatt
     2461 actccgcttc cggtatttcc tgcaccaatc acaccaactg ttttgttaaa cagcgtccta
     2521 ccgacgaact ccgcccgatc ttcccatcga tcgtccttga ctcttgcttg tgcttgtgtc
     2581 gtgcgtcgaa gcatggacaa caaattagtt atattatttt ctgctacagc atttcgttca
     2641 actaaagcag gtatgataga tacgattgtc tggtgttctt ttgctgcttt taaatcaata
     2701 ttgttgtaac caataccatg tcgggaaatc aatagcagtt catctttgtt ttcaaaaaaa
     2761 tctcgtcgaa aaaatggcgt aacacttgca ataatgatat tgaatccttt aagtaactcc
     2821 gctagttctt ttcctggaat ctcttgatca acagtaaaga aatgaacgtc tccaactgac
     2881 tttaatcttt ccaagtggtg tggaaagact cttccgaaac tgctggaatt aacgattgct
     2941 atatgataat ctggcatttt actcaccctt ttcaactatt tcatgtccgc caaacccatt
     3001 tcttagtgct gaaactactt ttgctgaaaa actatcttct tcctgtgaaa gattcctcat
     3061 gaacaaacta gttgcaatta ctggaactgg aacagccatt tctagcgctg tttcaaccgt
     3121 ccattttgct tcacctgatg cagaaacgat tccccgataa ttacttaaat tgggatgttc
     3181 gtttaactga tcttccatga tttccatcag ccagccgcga atcaccgaac catgattcca
     3241 aacacgtgcg acttgttcca agtcaaaagc atactcactt ttttgtacaa gttggaatcc
     3301 ttcaccgatt gcttgcatca ttccatattc gattccatta tggatcattt tcaagaagtg
     3361 tccgcttccg gatgcaccag catacagata tcctctttca attgctacat ctttgaataa
     3421 ttgttccacg gatagaaaag tttctttatc tcctccaatc attaagcagg cttgatgtcg
     3481 tgctccttcg attcctccag aagtcccaca atccaagtaa ccgatttcat ttttctgaaa
     3541 ctcttctgct cgacggattg aatctttata ataagagttt ccgccttcaa taataatgtc
     3601 tcctggtccg agttttggaa aaagttttct taacatgttt tcggttatct ctccagcagg
     3661 aagcattaac cagatgattt ttttctgctc aaaagtagct agaaattcag agatctcttg
     3721 ataagcagga atgtcatttt ctcttgcctg actaacagct gtacttgaaa tgtcgagtcc
     3781 ttgtactttg tgatggtgct cttttaagtt taaagctaaa ttcaatccca tttttcccaa
     3841 accaattaca ccaatttcca tgatagcgcc tccatttctt ataccctcat tatacaaaaa
     3901 aatttttttg taaatagatt ttacaaaaat attttttgtt tgattttttc ctactctcct
     3961 tgtatcttta acattaatga gaacggaaaa aatgataatc ttattatgaa aatgaggaat
     4021 tctcatgagc gtctccataa gactacaaat caaaacaatg tacaacgact tcagcccaaa
     4081 agaacaggct atcgccgatt atattttaga aaatccaagc aaagtaagtc atagtcctat
     4141 tagcaattta gcaagtgaac tgggcattgc tgactctact ttttttcaat ttacaaaaaa
     4201 acttggttat aatggattta aagattttaa aatggccatg ttgatccaag aaaatgatct
     4261 atctgctatt tctatccacg aaaatattca gaaaacagat actgagctca ctatggcgca
     4321 aaaggtgttc gagtctagta tttcaacttt aaatgacaca aaaaagttgt tacaacaaaa
     4381 ggatctaaaa aaagcggcta ctattatcaa tgaatctaaa cgtgtctatt ttttcggtgt
     4441 tggcggttca gagattgtag caactgacgc ttatcataaa tttttgcgtt cccctatcgc
     4501 aacttctcac agcacggatt accatattca attgatggaa gcttctttat tgacggaaaa
     4561 agactgtgct gttttgattt ctcacacagg gcaatcaaaa gaaacaatcc atattgctga
     4621 aaccgtaaga aaaactggag caaaaacaat cgttattact agccaagcta attcttccct
     4681 tgctaaactt ggcgatgtcg tttttatttc tatttctgaa gaaacagaat tccgatctga
     4741 agctttagct tcccgtattt ctcaactaag cattttggac tctctatatg tgattttgat
     4801 gttttacaac gaaaaagaat cacgtcgttc tatcgctaaa gttcgacata ctatttctga
     4861 aatcaaagaa tagcaccata acagaaaaag cgaaaaatcc ataaaacaaa aaaagccact
     4921 accaaatatt tggtagtggc ttttttggtt ctgcaagggt ggtacagaac atcaatagtc
     4981 ggtgggttag aacgtatgtc atcgcatctt tggagaaggt tgtattggta ttattcgagt
     5041 tacttgttag atgcgataac aaagttagta tacagtagta aaaaatttat cttttctttt
     5101 ttttattatt tttgtttctt attttctaaa aaattaaaaa aaattttggt gaattaagta
     5161 tttccttgat aacactggct tttggcctca ttttaggtta tcatttgtaa ttttatttca
     5221 cagttgatat gattgttatg taaaataaaa aagtttagga tacatcgaat ttttggggaa
     5281 attcttttgg gggtatccta aatcttttct attatattcc catattttct attattggtc
     5341 tagacgtatg atggtaaact ttgtacctat ctgattgctg aatcattaat tgcttatcat
     5401 tagccatctt tgtttcaagt tcgctagatg atcattttat tcacatccta acaaacttgt
     5461 cacatttata gattcttaat ctatttatca tacaattata aaaaaggagg tgctgcttat
     5521 gaaagctatc catatttctt tgcttttttt cgctagtgca ttgattttat ttgctattca
     5581 tctttgttcc cagccttata tcaataacaa cggaatatta gtggaaccag cattcttttg
     5641 cttaccactc ggctttttgt cccttcttgc ctccgcaggc ttcggatttg ttgctttttt
     5701 taaacgatca aaacaacaaa tctgacttat aaaatttacg taaaagagat cgtgacaata
     5761 atcgtgtgat attcccaaat ttcgaataaa aggtgcccct tgccatctct aactcctcag
     5821 tcacaagtta caatattcat aaaccatgaa aagcttgttt attgaaattg ctccttgtga
     5881 cttggagcta agtgacagat tacaacctct tttgcatcat aagactttac tccttgaaaa
     5941 acattactaa cttttgatat gtttcttgcg cttgtttatt gctgaaatca aactgatatt
     6001 catggttcaa aacagcattt ttctccgcat aatctacttt tgtgactttc acccccgctt
     6061 tctcaaatac agaagttact tcttccattt gtttcgtaaa cgtatttgta tttccatccg
     6121 ttaagaatgt gttttgcgtc caacgatccg catgtctgac caaggctaaa cttttgacct
     6181 tctctgattg ttcaaagtcc ttattatcaa agtaagccca tccacatttt tcataaagaa
     6241 agctagagat tttatcactt acttgagtaa gtgcagaagg atccaacaga ccagacaaag
     6301 aaataaactt cgtaatctgc ttgttctcca aagtcggatc aagatgatca gcttttttaa
     6361 tttcttcatt ttgaagtgct aacaaatatt ctccactaat ttgtgcacct gccgaatttc
     6421 caccaatcga aatattcgac caatctgctt gatcttttat ctctttttta gctgcttgaa
     6481 tggcttcgtt caattggata actggtgtag gatgttttgc ttccggtgat cgttcataat
     6541 ctatatttac gatgatttgc tgcgtgtctg aacatagata ttccaaataa tcacgacaat
     6601 catttttatc ccctcctaca tatgcgcctc catgcatcca gaaaagaatc ggtaattttt
     6661 tcttttccac tttcggtgtg tagatatcca tattattatt tttgtacttt gaaggatagg
     6721 atatttcttt ttttatatcg atttgttcga ttttttgctg gaaatcatct ggtcgggcat
     6781 atacttgttc tttttcactt gtgtcaaatt gttttctaac aagcaagcta atcggctttg
     6841 gtgtgatttg attaatcaaa aatacagtga ctaaaataac tacgatgata actggtactg
     6901 caatcactat tcgcttgaac catttcattc atttaccctc tctttcttcg ccaaatccat
     6961 gtgataatcg tgctaacaga taaaaatcca cctgaataaa tgagaagcga atgtttcttt
     7021 tctccagttt tcggcaacat tctttttttt gcatctgtct ttttttgtac tttcactgtc
     7081 atctgaatcg gttgcgtatc atcacttgat tgatcctctc gaggttcatt ctttccctta
     7141 ttgtctgcat tctctgtcgg atcttgcttt tttggtggag ctactgggat ttttttgtaa
     7201 tagaaaataa tagttggtat cgtctcatta aaaatccctt cagtcggtcc ttcaacattt
     7261 gctaattcgt atccttcgaa tgtcttttgc tctgtcatat aattttctcc ataagctccg
     7321 taaagctgga tttcgtcctg gtctttgagt agacttttat tgttttcatc cacatacttt
     7381 acgtttatct gatcatggaa cactactttc aaaaagtccg ttacatgttt cgtgtaggca
     7441 gcatggtcaa cttcctgtgc ctttccatgc cctgctcctt ctactaataa aagatcttta
     7501 taccctcctg ataactcata taatttttgc gcgttagaca caggtactac atcatctgct
     7561 gttccatgga taatcaattt aggcaatgaa tctgacattc caatcgtagg tagagcagct
     7621 tttgtatcca aatgcatctc ctgatcaaaa tagtattgtt tcaaaaggcc cataaataaa
     7681 tcttcatgct cttgatcccc aatgatgtct aactgatttt ttatggcatc cggtagtgcg
     7741 ggggctactg tctggctgta cgtaaaacgt agttgttcat caattgatgc aaatccgcaa
     7801 ttttcaataa ttcctttgac attgacagga atatctttcg ctaatacaga catcatcgta
     7861 gctgctccca tcgaaccacc ataaagtaaa atcttttgag atggccgctc tcgaacttct
     7921 tgatttatcc agtaaaggac gtcatctgac tcatattgtc cataggtcac ataatcgcct
     7981 ccgctattac cagtcgctct ggaatcaaca aataataaat tatacccggc cttataaaaa
     8041 tcattgtatt cttctgtaac gatcccgtta ttccaattcc ctcgaaatcc gccatggatc
     8101 atgactgttt tatctgaacc ttgatcgaca taatatgcat atacatccat cattttgcca
     8161 tcttgtttgt ttagtacagt ttgatgaagt tgttttgtat ttggatagaa gcctgtgttt
     8221 tctgtaggcg ttgtcccatc atctgtacct ccaagcatcg acatcaacac actgtttttt
     8281 gcaaaagcga tatcgtacaa tagataagta gcaatcagat gtttgataat ctctttatct
     8341 acaccataat cattaacagc aagctcagtg actaataact ctattaattt tgacttgatg
     8401 tcttctgttg gtccattaag tatttctgtc atctgtttac cgaagtaatt caaatattct
     8461 ttaccattga aattttcacc tttaccgata tcatcatttt ctgtcccatt ctctttgtca
     8521 cttactttag cagaagagga ttcgtcgttt gacatttttg tagctgttgc ttcttctgtt
     8581 tgttggttat ctgtggtagc ggtcgtttct tgatctgtgc tagtaacaga tgtctcaacc
     8641 gttgtttcag tatttgtttc tgtttgtgaa gtactcgatt tcgtttcatt tccagcatca
     8701 gtagtttctg tggtttctac ctcccgagat tccgattcgg aagaaatgct ctctccatat
     8761 actgtccatt gcgggacgct tgtacttaca gcaaataaga tgaccaacgc tccctttaat
     8821 acttgttttg ttctcaagct attcactcct ttttatgttt ctaggagaat tatatgtaaa
     8881 cgcaaacatt ttttatacaa aaaataaatg gtttatccca taaaaataga acaggattat
     8941 tttctcattt tacgtaataa agcagaatgg ataaaccagt atccattctg ctttttttaa
     9001 acgaagaaac aacttattgc tccaaaaatg tcagaaatcg tttatcccaa attttctcgt
     9061 aaaaagcgat tgtttccttt gctttcataa aaggattatc ataagttgtc gttgctcctc
     9121 tacacatcat aagctggtat cctaatccat gagcaaactt cacagtcgta tctatacaat
     9181 actctgcttg tgccccacat aattcgatcg aatgacagtc caattcagtt aataaaacac
     9241 tcaaattagt cttataaaat gaattcgcat gggtttttct cacaaaataa ctgtgctcag
     9301 gaatagataa ttccgacaat agctcccatg gttttgtttg tggcactaat tgagcatctt
     9361 cgtgttgaac gaaaataact ggtttcccaa tcgtttcgta tgttttgatt cttttattca
     9421 ctaatgctgt cactgtttct aattcgtgta tcggcgaaga atcaaaacat accccttttt
     9481 gtaaatcgat cactactatt gcgtcggctg tgtttgacat acttgtgcct tctttctttt
     9541 ttcttgattt aatagtattt cttatctagc tagttccatt atacgtgaca acattttctt
     9601 attttacacc tctaaatggc acttttcgac tatttcttca tgttattatt tcataaatac
     9661 ttagctattg gaggaatgta tgatgattaa attgtataca ggttctggct gctcgtcttc
     9721 aagacgcgca aaacgttggc ttatcgtcca tgatataccg tttatcgaac agaacttaat
     9781 tcgtcaacca ctaaccaaaa atgaatttat ccacttacta aaattgacaa acaacggcgt
     9841 gaccgaaatg ctctctactc attctattag ttataaaaat cttgttcaca gaatagatga
     9901 actcagtttg agtgaactat atattgagat tcgtacatcc ccttctttat tgaaaaaacc
     9961 gattatctac gaccaatctg caaaactttt gactggattt agcgaagata aaatcaggat
    10021 gttcctttct aaaactaaaa gaaagcaaga gctcaacgaa ctcttgcttt ctggtcatct
    10081 tgtcgatttt aaagaaaata atctttttgc aatgtcttaa ttttggcata aacaatctgc
    10141 ttttcttaat taacgaaatc caatttttcc aatatggatc tatttttctc attcctgatt
    10201 tttatgattt cagctttcag acgcgcccaa tcattttttg taggttgtcc attccagtaa
    10261 aatatctgtg tattcgtttg atcatatgcc tttttgtcga taggataatc gctaatgaca
    10321 atatcaggta actgatcagc ggatgcacta tattttattg ggcgttcagt catagataat
    10381 atccttttct ttgcccataa ttcctgtgtt ttaccatact ttgattgaat atagaatatg
    10441 acaggttgtt cgtgttttat aaaaatatcc ctgactaaca tacagtattg aaaaatcaac
    10501 cgtctgttct tttcataata agtattaagc tctggaattc tgcgagctac cccactaaaa
    10561 aactgttgca tgattggata catcactgga aatatatgct gaaaatctct gtcttctgct
    10621 gattttccaa aagcatccac cttctttttt gtcccaaaaa cccttgaata caaatggatc
    10681 attgccaaat tgacatatag ataatttttt tctgaagttg taaaacaaat cttgaagttt
    10741 ttttcaaaat tttctacaat agacgattca atccagcccc aactttcttg tactttatct
    10801 ggagtatcca acaaatcttc ttccaaatag gtgttcatta caccaaacat aaaataaagg
    10861 taagcttgct ctgttggagt gatgtgattc atcgaccaga agtttgcttc aaaaaaaggc
    10921 tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg gatgcacaaa tttgttcatc
    10981 tcttctggga catacgtaat cgagtggcct tgtttcattc gttgcaacga aattcttagt
    11041 aggctgatcc atttctttct atctatacta cgcgtatatg gcgcatgttt cgcaaatttt
    11101 tctaatagtt gacgtttatt ttttatactt gataatatat cagaatgatt ttcgtcgaat
    11161 gactgccaat acatcaagtg aaaaaagtat cggatacggt attcttctcc ttctatcgga
    11221 ttaccttttt tcaagctgca agataaatga tagtttttca aaatagtatt aagttctctt
    11281 aattttcttt gaatcgtacg caaacttata taattttttg actaagattt tctaaggatt
    11341 gataagtacc tttgtaaagt gaatccagta aaatataacc aatcgttccc tctaaaagta
    11401 tagaataaac tttactcagt aaaaactgag aagaaaactc gacttttatc atcttgtttt
    11461 ctgaaagaaa agataattta tattctggat acctttcgca aaacgattct gcttgttgaa
    11521 tcaccgaatt catcttagcc agggtgtagt ttagatctaa agcgatatcc ttcaccacat
    11581 ataatttctc gtctctttca taacaatagt tcaaaaattg atatacttgc ctttcattga
    11641 tatcaataaa ttcttcaaac aaagtacata ctccttttta tctattattt agacttattc
    11701 cgacagctac atgaataacg tacaaataga ttcgttactg atttttcaga gatactacca
    11761 tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc catttgtgtt cctgttattt
    11821 cctgtttctc aaatatgtta gtaaacaaaa atagatctgg caaagctaca acttcgccag
    11881 gcaatacagc actctcgaat gaaatattca ctcaaaaatc tatcgtttta ttcaatttta
    11941 gatctgttgc ttttattctt tatttcactc gatggaagag agaacgaaaa tagcttgctc
    12001 ctacaatcaa aatgagtaac acacccatcc atgtaattag cttgttcaag gttctactac
    12061 cagtttctgg taaatcgctt tttctattat ctgtttcttt cgctttttct tgatgatcaa
    12121 ttccacgaga aacaaaatca ggctgtttta gcacactttt taggttgctc cctgcatttc
    12181 tcttttgttc cttagttggt atgataggta ccaaatcaag ttcattattt acttttcctg
    12241 tgctgctttc gctgctcgtg cttcctgtgc tactctcact actc
//
LOCUS       DBNIBO010000086        12171 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.090, whole genome shotgun sequence.
ACCESSION   DBNIBO010000086 DBNIBO010000000
VERSION     DBNIBO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.090"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            335..1246
                     /locus_tag="AC1Y77_001838"
     CDS             335..1246
                     /locus_tag="AC1Y77_001838"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1575403.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1463..1900
                     /locus_tag="AC1Y77_001839"
     CDS             1463..1900
                     /locus_tag="AC1Y77_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575404.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            1995..2879
                     /locus_tag="AC1Y77_001840"
     CDS             1995..2879
                     /locus_tag="AC1Y77_001840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIQ1575405.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3103..6270
                     /locus_tag="AC1Y77_001841"
     CDS             3103..6270
                     /locus_tag="AC1Y77_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIQ1575406.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6338..6835
                     /locus_tag="AC1Y77_001842"
     CDS             6338..6835
                     /locus_tag="AC1Y77_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HIQ1575407.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            6854..7318
                     /locus_tag="AC1Y77_001843"
                     /pseudo
     CDS             6854..7318
                     /locus_tag="AC1Y77_001843"
                     /inference="COORDINATES: protein motif:HMM:NF023225.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            7315..8073
                     /locus_tag="AC1Y77_001844"
     CDS             7315..8073
                     /locus_tag="AC1Y77_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HIQ1575408.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8159..8899)
                     /locus_tag="AC1Y77_001845"
     CDS             complement(8159..8899)
                     /locus_tag="AC1Y77_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIQ1575409.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8960..10213
                     /locus_tag="AC1Y77_001846"
     CDS             8960..10213
                     /locus_tag="AC1Y77_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIQ1575410.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10535..10762
                     /locus_tag="AC1Y77_001847"
     CDS             10535..10762
                     /locus_tag="AC1Y77_001847"
                     /inference="COORDINATES: protein motif:HMM:NF013777.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIQ1575411.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10885..11211
                     /locus_tag="AC1Y77_001848"
     CDS             10885..11211
                     /locus_tag="AC1Y77_001848"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIQ1575412.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11279..11992
                     /locus_tag="AC1Y77_001849"
     CDS             11279..11992
                     /locus_tag="AC1Y77_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HIQ1575413.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         4219 a         2020 c         2514 g         3418 t
ORIGIN      
        1 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
       61 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
      121 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
      181 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
      241 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
      301 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
      361 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
      421 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
      481 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
      541 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
      601 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
      661 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
      721 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
      781 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
      841 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
      901 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
      961 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     1021 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     1081 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     1141 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     1201 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
     1261 aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat
     1321 caacagtagt ttaattacta cttgatttag ttctttttat tttaaaaatt ttttttactt
     1381 agaatccagc gaccctagat atttttctgt ataatcttaa ataaagaaaa caatatcaaa
     1441 aaatgcacgt agaagtagat aaatggagtg gttttgcaaa tttcttttat ttctagaagt
     1501 tattcacgta tacggatttg tttttctcac aaataaaaag gcaggaagta caatggatca
     1561 tttgtatata aaatatattc atcaattttc cgttttggtg ttaggcacac taattatagc
     1621 ggggattgtc gcatttatta ttcaaggccg tgacatatta acagtacatc aagagttttt
     1681 cagttatgca tttggtattc ctctgatcgc tataaggtct gataacaaaa agtggaagaa
     1741 acgctattac attctcttgc tatttttgtg tttttatctg ccacctaaga tttttacttt
     1801 attaggtata caatgcctat cattttcttt tatttcctat atcattgttt taattttcct
     1861 cagtttcata gtatggatgt tttatcttgt caaaaaataa atgaatgttt catgaacagt
     1921 acttcatcac agcctaattt ttttctttat gctatcgtta tattaaagaa atcaatcaga
     1981 agggaaagaa agtgatgaaa aaagttttat tagtggtaaa ccccagttca ggcggtgaac
     2041 aagcaaagga atttgagcaa ttagcgatag caaaattgga atcggtgttt gatgaagtgg
     2101 tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa gcggctacgg
     2161 aagggtatca tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg
     2221 gaatcgctga acaagaacat cggccaaatt tcggattttt cccattgggt acggtgaatg
     2281 acttggctcg agcattaggg ataccgctag aacctgagga agcgatcaat catttttcga
     2341 ttgaatcagt caagccattg gatattggaa aaataaatga cgactatttt atgaatgtcg
     2401 tcgcaattgg ctctatccct gaatcaatca atgatgtaga tgcggagaag aagacgaaat
     2461 ttggaaaact agcttatttc atgtcaggaa tcaaacagtt agcttccaca caatcctact
     2521 cgtttcacgt agaagttgat ggaaagaagg aagaaattga gagcagtacc ttgctgatag
     2581 gactaactaa ctcagtcggc ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg
     2641 gtaagctcca tttggtttat ttaaaagatt cttctttgat cgatacgctt aaaacattgc
     2701 cagacctatt aaaaggagta gatgaatcta cgaaaaatct agcctacctt acttgtgagc
     2761 aattagttgt gtcattagct gatgaggaag aattgacgac gaatgttgat ggagatgaag
     2821 gggataaatt acctgtgaca atcaaagtat tgccctcaca tttacaggtt tattgttaaa
     2881 atggaggttg tgaccgagga gttggcggga gaacaccgct tgttcgggat aagggactgt
     2941 gacaagactt ttgtcacagc cttttttttg tacaaaaaag aaatttacac ctaatagaaa
     3001 aatttttcca taaaaataca tattttaaat atgaaagtac aattggaaat agattaaaac
     3061 gataaaataa aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa
     3121 aagctgattg cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc
     3181 gatgtagaga aaacaaacga atcaacgcaa tcagagaatc aaagttttga caagactact
     3241 ttttcagaag aatcgattac ttctgaaact acagaatctc aaagtgaggc ttctactaat
     3301 aaaaggcaag aagaaaataa gacaagtgag acctcagaag tagctgaaca aacgtctgat
     3361 agcagtgaag cggcactggc aagagctgtt tcagcggatg ggtacagcga agctgccaca
     3421 acagaagaac tggcgcagct tctcgcagac gaatcagtaa ctaagatccg actgattcag
     3481 cctttgactt tagacaggga attagagatc aaacgagata tcgttattga ttttggtgga
     3541 ttcgctcaca atttcggcac acaccatatt tatatcaatg agatacctca gtcgatcgag
     3601 ttccagaatt ttaaaggaac agctgcacat ccaggtggat tgacaccaaa tatcgatgga
     3661 aatgccatta tcattgctta tatgaccgat ttttggggaa gcagatatca ttttacggga
     3721 gaaataaaat ttaccgggac acttgatttg tatgatgggt ccaaattagg actgatttac
     3781 gctcctcgtg caactgtcac attagatggt gtttccggtg tgttggatgt ccaaccagtg
     3841 aaggaaggac tgaatgccgc tccgggtaaa gcctattttt gtagaagcta tcaattgaat
     3901 gtgatcaatg gtagtcaact ttatggaccg tatctaggga aattctacgg ttttctagat
     3961 tcaggagatg agactggtag tggaaaaaca gcgccaggaa tccatatctt aagcggttca
     4021 aaggtctctc ttgattatga tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat
     4081 acgctacctg gtaatgtcat gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat
     4141 acaaagatca atattaccaa tgacaacaat cgcggaatca tccaaatgag aggttcagga
     4201 tcgaaagtca ctgtatcgaa tggtgggaaa atcaccatca atacagcaac tacgggcgga
     4261 tttcgcttgc aaggagattc tagtcaaatc catgtatcaa gtggtggaca aatctcggtc
     4321 aaaatggctg gagatgacga tcaacccggg aacaatggga tgcgttttgt aggaaaaggc
     4381 ctttctttga tcgtagaagg agccggatca gttatcgaca tcgataagca gagtgggcgt
     4441 tccagcgcag ttcgttttga gaatggtacg caaaagctga cagtaaccaa tggaggctca
     4501 ttgagggtaa gaaatacagg tgatggcaaa tcttacgtga acagactcaa tcaaggaaat
     4561 caagcgatcc aattttatga cgcttctggt tttctagaaa gtccaggttc agctgatttt
     4621 acagtcactg gagaaaaatc aaatatcgat atacgcgcag attccggagc aactgtagat
     4681 acaactggag atattgattt aaattttacg gcaggtgaaa aaacttattt agtcatggtc
     4741 gggaaaacag gggaagataa gaccggcatc atcagtgggg atgtgttgaa tgtggactta
     4801 gaatctccta cctattttga tttccaaaac aaacggattg gaagcgagac caatggcata
     4861 ggcggctggg tattccaagg aaatggagat tcttctttaa agatcgccca ttcagaaatc
     4921 gctttatggc gaaaaacagg cgcaacattc gataactttg aaaaagaccc ggattatttt
     4981 tctgatatga cagacttatc gatcaatggg accgacttag gcaatttcgt atcaagccct
     5041 agtgcaggtt taacaaatga atttgctcag acaggtgccg gaatgaaaaa ttataatcga
     5101 atcagtgcca ataatcagct cccgatgatt gaatccttaa gagtgccaac gaatgcagat
     5161 aaaaaaatct atgggcatgt cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct
     5221 tggacaggcg aagtagaagt gactttacgt attacttacg cagatacgac gaaatcatct
     5281 gtagaaatta ctgctctcac gcaaggacaa acagatgaaa atgaaggggc aggatataac
     5341 ccatggggag aaggcgaaca aggcggcttg ttcgaagttc aagtacccaa tgatgagaga
     5401 ttaaaaacgg aggatcaagt gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa
     5461 gtgacagaac tggactctgc caaaacgaca gtcgatgtga ttcctcctga acctgctaat
     5521 ttaagtacca cattgatcaa tgcagcttct cgcacgatca caggtacagg tgaagcagga
     5581 gccttggttt cattgaaaaa aaatcaagct gttttgtcag aaacaactat tgatgataca
     5641 ggggaatttg ttctttctat cccagaaaat caactttttg caggagatgt attagaaatt
     5701 gtattaaacg accaagcagg agaagctact gctaagggtg tgcagaacaa gccaagtacc
     5761 aacgatgaaa ttgggaatca caatccaagt gatacgccag ttgtttatca tgatgccccg
     5821 ccttttgcac ctgctacgaa gatcgtagta gaaggtggtc tctcatttct tgccccttcc
     5881 aaactcgatt ttggtcagat caaagcaact ggtttgacac aagaagcatt tggccaaatg
     5941 gatatacagg aaaaattatg gatttatgat cagcgagaaa cgaaaaatcc gtggatctta
     6001 acgataaaat tatctcagcc tttcacaaat gatgcaggat tttctatgca ggatgtccta
     6061 tatttcaaga aaggagagga gtatcaatta gtgaatgaag aagaactgga aattgtcaga
     6121 ggacaaaatt caacgaatgt gcaagcagat tattcagaat atttaaataa cggtcgggcg
     6181 tttaaacttg tcttaaccaa agaaaatcag atagctggta attactcagc agagataaca
     6241 tggacattag cagatgcgcc gacagaataa agatcaagga ggatgattga tgaatcgaat
     6301 atggaataaa agtcatcgaa acaactgctc tttacttatg atccttctcc ttggattcat
     6361 cctttggcag acacctgtcc atgcctccga attcaatttt gcggtagatt ttgaattacc
     6421 ggctaatcag attgataaga acaaaacata tataaatgta aggatggagc ctgggaaaac
     6481 acaggaaatc ccctacacgg tctttaaccg aacagataaa tcagtaacag ttgagacatt
     6541 gatccactcc gctacgacga attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa
     6601 atttgatgaa acattaagtt atgatatgaa aaacttagca aaaacagtgg gagaaataac
     6661 tgttcctcct caaagtgcgg tcactgagac actttcactg aaagcgccag aaaaagagct
     6721 ggctggtgtg gtcgcaggag ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa
     6781 aagtacagtt ggtgtcgtca atcagttttc tcaagtgaaa gcaatcttga tttgaaatag
     6841 cgaaaaagag tcttccccag atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg
     6901 aagaaatgtg attaaggcga atcttcaaaa tccaaatgct tcttatatgt tcggcttgac
     6961 tacatcggtt gaaatctaca aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga
     7021 aaaaatagat gtagcaccca atacaaattt tgatttactt gtcccattag aaggaaaaaa
     7081 attagctccg ggacattatc aagcggttgt gaagggtgct tggaaagaaa aacgctggaa
     7141 tctccatacc gattttgaaa taactgatga tgcagcggac caattgaatg aaaaggatat
     7201 tgatttaaaa aatcagccag aaacatttga ctggcgatgg ctgatacttg ttggagtcat
     7261 tcttattggc ttatttgctg gattatttat tttaaaagaa agaaggaaaa agaaatgaaa
     7321 aaaacaattg cattaagtac cgttctagct ggaagtctat tattatgtac acaaagtgtt
     7381 tttgctgaga ataagagctc agccactaca gaaggaacga tttctgtgac caaaccagat
     7441 gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg atccaaatga tcctgatact
     7501 ccttttacac ctgacgaacc aaatcaggga acatccggat tactgacgat tgaccaagct
     7561 ccagatcttg attttggaac aatcaagtta ggtacagcaa aaacagtata tgctgcatta
     7621 caaagcggaa cggacagtac tggagcacaa acacaagtac agaatttcgt acaagtaacg
     7681 gataaaagcg gaaattatgc cggttggaaa ttatcagtca aacgaacaga atttattggt
     7741 acaacatctg atacgaataa actaactggt tcaaaggtga aattcaaaaa cgccgtgatt
     7801 cgaaatagcg caactaatag cagtggaatg ccatctaccg tttctggaac tggcgctgaa
     7861 ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg
     7921 attggcacat ggatttattc attaggagag aatgtggaac aagggagaca atcagtggaa
     7981 ttatttgttc ctgtatccaa ttatgctgta gacaactacg tttcaacatt cacatggtcg
     8041 ttaacagatg ctcctacaga agaggaagcg taaaatcata tcaacgattt cagggagtgg
     8101 agccgattca aggcccactc cctgaaatct gctatgctta cgtctcagct cttattcatc
     8161 aactcgaagc agttttttgg aagtcatgga aacgttcctt tgtcttttca aagatctttc
     8221 gaccaacgag taaggagtca ccgttgaaaa attctaacac ataatgagca gaatcaattt
     8281 tctgcaattg cgataagttg atacagtaag attttaaaag taaaaagtaa gcatactttt
     8341 ctaactctgt cttcaattgt tttaaagaag tattcactaa tttatcttct gtagttgttt
     8401 tgaccataac ttggtttttc aaacctttga tactttcaat atataaaatt tcccgaggat
     8461 acaggacgag acttccacct ttttgatcaa taaagatttt atcgacattt acgatctgct
     8521 gttctttcca attgatctgt tctagcactt gacgaatttg ttcagataaa ttcctgtctt
     8581 ttctgatata tccaatcgga aggattttcc gattgatgat ctctactgca aaattcgtat
     8641 aagcggtgaa aaaaacaata ttactatgtg catttttttt acgtatttgt tcggcgacat
     8701 caatacctgt gagtgattgc ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa
     8761 tagtgcccaa ctggatcaac tgattgattt gagagataga agtgagctgc acgatattct
     8821 cgatatgatg attgaccaat tctctgatga tccattcgct gtgaaactgg ttatcttcta
     8881 tgataaaata ctttttcata tgcaggctcc tattaaaaaa atatgtgtac attttgaaac
     8941 aaaacaatgg aggaataaga tggtcgcaac agtgacactt tatttgacct ttactttgat
     9001 tttagattta ggtatctatt tattcttttc gaaacaactg ctagtaaaaa acagctttgg
     9061 ttgtatagct ttgatggtgg tgacactttt acttcctatc cctaaagtga gctttcctta
     9121 cctagttggc ttattattga tgtttctttt ttattttact acgaaaaacc tgtttatcag
     9181 ctgtttgatc agtaatcttt tttttgctat cactggactc agcttttttc tggtttttga
     9241 tctgatggat tatttaggaa agagacatct tggaacagca agtttttttg cactacttat
     9301 cgcatttatt ttctatctat tagtaaaaag tatagacaaa cagacgagat ttctcaactt
     9361 gctttttact tatccaactg aacactattt tggaggtatt tgtattttat taagctgggg
     9421 cgtgatttac ttggtattgg gaagtgttca cttccgctca tctaattatc tgcttctatc
     9481 tcttttgtcg atagtcatca gttttttttc tttgtttttt tccattatgt tgatcagttt
     9541 ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg tatcaaataa atgaagaata
     9601 ttaccatcat ctagaggaat tcaaacatga ttataaaagt ctactgtttt cattaaaaat
     9661 tactcttcag gaaaataaaa aagaaacgtt ggaattttta gaaatattag aagatcattc
     9721 tgcagctatt tttgaaaaac cgcaaataca acagctaaca aatattgtat ccccagctgt
     9781 caggggtgtc tttttaaaat ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt
     9841 agttattcct gtcgaagtga aaacaatccc gattgatctg gttgttttta ttcgctgttt
     9901 atctattttt atttctaacg cattcgatca tcgtgtagcc gatcagagtc cgattacgat
     9961 caatctgctg gaagaaaaag agggatcacg ttttagtata gcgaacaagg caaatccaac
    10021 gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt
    10081 aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa aatgcagaac taaaaatcga
    10141 attttcaaaa cagaatcact atttttttgt agaaatgtat ctggcatcta gaaagagcaa
    10201 atcagcctac tgacttactg tcaaaggata agtttgagga aagtctacac ggagatagag
    10261 acaacaacac agcatattca aaagaaacag aagcgtgatg accgttgtca cccttctgtt
    10321 tcttttgcta tgtagttaaa caaagtagtt gacacttccg ctacttagta ttagtacgaa
    10381 agtatacttc atgatactaa gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa
    10441 tgctaactgc tttgcttaca ccagataaag ggaagagcaa tatcgcttat tttaggcgtg
    10501 gagaagcaag caaatcccta cgatcacaaa aaaaatgctg acccagcagt taagagagtt
    10561 agaacaagat gaaagtatcc atcgaaaagc ctataaccaa gtcccgccta aagtagagta
    10621 ctcgctcacc gaagaaggaa aatcattaag agagattcta gtagcgttgt ctgtttggga
    10681 agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa gacacgctac tgcatacaca
    10741 tggtggctac ctaaattatt gattttctat gacatttatt ttaaataaaa gaaaacagga
    10801 gtttttttaa aagcatcatc tattttttat gtaaaaaaat ggtatgatta atcaagtaag
    10861 aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa aagtcaacca gtttagagat
    10921 gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg ctatctctat ttctttggaa
    10981 gcatctgaat tgttagaact ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa
    11041 ttagaacgaa tcaaagaaga actggcagat gtattgatct attcatacat gatggcagac
    11101 aaccttcacc tggatctaga tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa
    11161 tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca cagagttgta gaaatgtgtc
    11221 attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat
    11281 gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc ctcttgttcc tcttgttatt
    11341 agtaggacta gctttgattt tcaatgaaca gatcaaggat tattttgtca gagagacagg
    11401 agataaatat gctatagcga atgtaacaaa agaagatctg aaaaagaata acgataaaga
    11461 tgtcagtttt gactttgatg cggtagaacc aatgacgaca gaaggggtca tgaggtcgca
    11521 aatgagagga acagatctac cagtgattgc tagtatcgca gtcccttcag tttcaatcaa
    11581 tttaccggtt ttcaaaggct tggataatac gtcactactt tacggagcag gaacattatc
    11641 tccagatcaa gaaatgggaa aaggaaatta tgccttagca agccatcgtg caacgaaccc
    11701 tgaattgctg tttacaccgc tagaaaattt agagatgggt gccaagatct atttgacaga
    11761 tttagaaaat gtgtatacat acaaaacatt ttttaaagaa aaagttgctc ctacagatac
    11821 ccaattactg aatgaagtcg agggaaaaga aattgtcaca ctaattactt gtggcgacat
    11881 ggacgccgtc acgcgactag ttgtacaagg agaactggaa agtgttacat cgataaaaga
    11941 tgcgacagat gatatgagat ctgctttcaa tctagagaca aaaacatttt aggatactgg
    12001 gacataagtc gttacagatt aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa
    12061 tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg atttggttgt tttctagata
    12121 gacttgtgtt ccagtttttt tattttttat atgaaaaggc ttgcataaat a
//
LOCUS       DBNIBO010000087        12106 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.091, whole genome shotgun sequence.
ACCESSION   DBNIBO010000087 DBNIBO010000000
VERSION     DBNIBO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.091"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(68..232)
                     /locus_tag="AC1Y77_001850"
     CDS             complement(68..232)
                     /locus_tag="AC1Y77_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575414.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            527..1855
                     /locus_tag="AC1Y77_001851"
     CDS             527..1855
                     /locus_tag="AC1Y77_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIQ1575415.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2057..2890
                     /locus_tag="AC1Y77_001852"
     CDS             2057..2890
                     /locus_tag="AC1Y77_001852"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIQ1575416.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2906..3643
                     /locus_tag="AC1Y77_001853"
     CDS             2906..3643
                     /locus_tag="AC1Y77_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HIQ1575417.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3798..4766)
                     /locus_tag="AC1Y77_001854"
     CDS             complement(3798..4766)
                     /locus_tag="AC1Y77_001854"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIQ1575418.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            4998..5831
                     /locus_tag="AC1Y77_001855"
     CDS             4998..5831
                     /locus_tag="AC1Y77_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIQ1575419.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5865..6704
                     /locus_tag="AC1Y77_001856"
     CDS             5865..6704
                     /locus_tag="AC1Y77_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1575420.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6730..7290
                     /locus_tag="AC1Y77_001857"
     CDS             6730..7290
                     /locus_tag="AC1Y77_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575421.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7489..9210
                     /locus_tag="AC1Y77_001858"
     CDS             7489..9210
                     /locus_tag="AC1Y77_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HIQ1575422.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9375..10517
                     /locus_tag="AC1Y77_001859"
     CDS             9375..10517
                     /locus_tag="AC1Y77_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIQ1575423.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10533..11744
                     /gene="thiI"
                     /locus_tag="AC1Y77_001860"
     CDS             10533..11744
                     /gene="thiI"
                     /locus_tag="AC1Y77_001860"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIQ1575424.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4125 a         2034 c         2518 g         3429 t
ORIGIN      
        1 atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta gagttggcta
       61 ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc attcctacac
      121 caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta tctgtattca
      181 taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc attttattca
      241 tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa gctatcttat
      301 tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt atctcataga
      361 aatattcctt tttatctgaa aactattgta taccgtatga catttttctt catgaagttg
      421 taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt tctaaattct
      481 taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga aagctattac
      541 gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac agcaggcatt
      601 gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt cagcacatgt
      661 ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta atcaaatgca
      721 atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga tgctgaatca
      781 attccattta aaagagtttg aattatcaca aaattacact tttttctggg ataaatatga
      841 aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa tcgatagccg
      901 gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg atatgatcgt
      961 ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa gtgcgaatag
     1021 ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag atgcagtagt
     1081 gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa agaaagaaga
     1141 aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc caaaagaatt
     1201 cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac tgacaccaca
     1261 aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg tgatcaatgc
     1321 accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag gaaatgtcgt
     1381 tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa aattagcgat
     1441 tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc aatcttctac
     1501 tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat ttgatacgga
     1561 ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga cccatgcgat
     1621 ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag tcgaaaatag
     1681 ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct ggatggacga
     1741 atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat taaatgcgtt
     1801 caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag catagacgag
     1861 tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca gctcttttta
     1921 tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa ataaaggata
     1981 aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa taaaattggg
     2041 agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat taaatggtaa
     2101 agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa ccgaacatcg
     2161 tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag cagaagtaga
     2221 cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc taggtgaaac
     2281 aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg atcaacgaga
     2341 tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg gttttcgtaa
     2401 agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt ggtggacttt
     2461 tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga atcgatggaa
     2521 tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat cggttgaacc
     2581 aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg tgttctttga
     2641 ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc gggaacaaga
     2701 tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg cttatcaaac
     2761 tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt tatattatct
     2821 tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc aattactaga
     2881 aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc atgaagtttc
     2941 atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag gagtagttat
     3001 caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg tcaatagctt
     3061 tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc ctagagtcgg
     3121 tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct gttatcggaa
     3181 cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg ttttcgtatt
     3241 gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg ctccttttga
     3301 gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg attcgggctt
     3361 catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg ttaataatgc
     3421 gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta cgcatgttcc
     3481 agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga tcgaaagcca
     3541 ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc gaatgcaaga
     3601 acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag gttgtgacag
     3661 aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca aatttttgta
     3721 aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt ttaagtttag
     3781 ctattagata atgatgttta gttttggata aacgaaatca attcttcacc cgacaaacca
     3841 tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact gttacagaaa
     3901 ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac aggctctgcg
     3961 attccattga aacaacgaga aacaagtact acagggattt tttggacaag aagcttttcc
     4021 aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat taccaaaccg
     4081 tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc atatgcttta
     4141 acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc taaaagttcc
     4201 tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt aggtgttcgg
     4261 aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg gatctcatcg
     4321 ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac acgaatcgca
     4381 ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat tgccccagtc
     4441 aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt ttcttctaat
     4501 gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc ggacaagata
     4561 cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag attgaagata
     4621 tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa taatgggttt
     4681 gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat cgttccgccg
     4741 gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat tttgcatagc
     4801 cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga ataacgaata
     4861 gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa ccttgacctc
     4921 ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa ggaaatcaat
     4981 cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa catgacaaat
     5041 taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc ttagtggaat
     5101 tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg ataagtgaag
     5161 tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt caaggagact
     5221 ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat gttatgtcgc
     5281 caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca tcattcaaat
     5341 gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag ggttatcctg
     5401 tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa cgtacaagaa
     5461 actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag ttgctgaata
     5521 caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt cgattccatg
     5581 gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa agaacgttat
     5641 atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta gttgattcta
     5701 agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg gctgaaaatg
     5761 cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca acccaaatgg
     5821 atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc aaagctattt
     5881 tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa tcaacaaaac
     5941 aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg gggcgcagcc
     6001 cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc gtgtataatg
     6061 gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa caaaaagtat
     6121 tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt ggtgcgagaa
     6181 atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc aagcgactag
     6241 tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc caggttttta
     6301 gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa ccaatttatc
     6361 aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc tttcccgagt
     6421 gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt ggttcaaaat
     6481 tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa attatggcat
     6541 tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga gtggccatgg
     6601 ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca aatgaagaag
     6661 atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg aaagaaggaa
     6721 aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca caaaactttt
     6781 gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca tacacgtgct
     6841 gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa taaagaacaa
     6901 ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga aaaaatactt
     6961 ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact gacagatatc
     7021 ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg aatattcgaa
     7081 atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca ttatgaccct
     7141 gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc agaacctaaa
     7201 atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa atccgctgat
     7261 ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg ttatctttat
     7321 acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat agtatgctat
     7381 aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg ccaagcagaa
     7441 cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat gggaaataat
     7501 gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt tttggttagt
     7561 tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg caaggaagaa
     7621 ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca tctggttggg
     7681 caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc tacttcctcg
     7741 ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag ttttcggttc
     7801 ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat ggagaaacaa
     7861 gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg caactcacta
     7921 gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag agaagacgag
     7981 catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat cgaaaaagaa
     8041 tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg cgaggttttg
     8101 gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat tccaccgctg
     8161 tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga tacttacgag
     8221 aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga tatcgcaaaa
     8281 gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt tgaaacggtc
     8341 gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat cttagaacgg
     8401 gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga gtatatcaaa
     8461 catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc tcaaagctat
     8521 acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt ggaagaaatg
     8581 gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc ttattcagaa
     8641 gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa gcaacaagta
     8701 gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca agaaaagatc
     8761 gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca acgtttgcct
     8821 ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat tgaagaacta
     8881 agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt agttgctcta
     8941 tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga tgcagcagca
     9001 ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga agaagtgaga
     9061 gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca agaagcatta
     9121 gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg catcgaagat
     9181 ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc agcaagagca
     9241 agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga aacaagtgaa
     9301 aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag aatttcaaaa
     9361 aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc tttccgcaaa
     9421 gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca agcttgcatg
     9481 atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc gctgatttga
     9541 tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga gataactggg
     9601 tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc atttcaggag
     9661 tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga atcgaagtaa
     9721 gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa gaattaataa
     9781 gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga tcgattcagc
     9841 ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc catgtagatg
     9901 ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga gttgattttg
     9961 caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt tattggaaaa
    10021 agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat cagcgaagtg
    10081 ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta tcattagaga
    10141 taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc aatgcgttga
    10201 agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg catattttat
    10261 gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa gaaaaacaaa
    10321 tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc agtacattgc
    10381 acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc ttagatgaaa
    10441 acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt tataagaaat
    10501 tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt atggtccgtt
    10561 atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa ctagcacaaa
    10621 atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat cgggaccgga
    10681 tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta gcaaaaatat
    10741 ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa gttttgaaac
    10801 aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt aaaattatcg
    10861 ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa acattaggta
    10921 atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca gatattccat
    10981 tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc aagggagctg
    11041 gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg gggattgatt
    11101 ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct gttcattttg
    11161 ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta acagccaaac
    11221 tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag attcaagaag
    11281 aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga atgatgttgc
    11341 gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat ggtgaatcat
    11401 taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa gtaacgaata
    11461 cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa gtagcagaac
    11521 agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg atttttgctc
    11581 ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa gagcgtttag
    11641 caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag atacaagcag
    11701 aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt atgaaaatag
    11761 tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta ttttaccgtt
    11821 tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt tatggaaaag
    11881 ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc tacagttgat
    11941 aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga agtgggcagc
    12001 ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga aatttcggct
    12061 tatttttcga agaagttact tctggaacac cgtgtatcag aggttg
//
LOCUS       DBNIBO010000088        12074 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.092, whole genome shotgun sequence.
ACCESSION   DBNIBO010000088 DBNIBO010000000
VERSION     DBNIBO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.092"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(133..1440)
                     /locus_tag="AC1Y77_001861"
     CDS             complement(133..1440)
                     /locus_tag="AC1Y77_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HIQ1575425.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1443..2024)
                     /locus_tag="AC1Y77_001862"
     CDS             complement(1443..2024)
                     /locus_tag="AC1Y77_001862"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIQ1575426.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2140..2237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2596..3888)
                     /locus_tag="AC1Y77_001863"
     CDS             complement(2596..3888)
                     /locus_tag="AC1Y77_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1575427.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3885..4508)
                     /locus_tag="AC1Y77_001864"
     CDS             complement(3885..4508)
                     /locus_tag="AC1Y77_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575428.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4511..5347)
                     /locus_tag="AC1Y77_001865"
     CDS             complement(4511..5347)
                     /locus_tag="AC1Y77_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575429.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5364..6251)
                     /locus_tag="AC1Y77_001866"
     CDS             complement(5364..6251)
                     /locus_tag="AC1Y77_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575430.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6267..7550)
                     /locus_tag="AC1Y77_001867"
     CDS             complement(6267..7550)
                     /locus_tag="AC1Y77_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1575431.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8233..8916)
                     /locus_tag="AC1Y77_001868"
     CDS             complement(8233..8916)
                     /locus_tag="AC1Y77_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIQ1575432.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8934..9551)
                     /locus_tag="AC1Y77_001869"
     CDS             complement(8934..9551)
                     /locus_tag="AC1Y77_001869"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIQ1575433.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9561..10385)
                     /locus_tag="AC1Y77_001870"
     CDS             complement(9561..10385)
                     /locus_tag="AC1Y77_001870"
                     /EC_number="3.4.-.-"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIQ1575434.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10378..10938)
                     /locus_tag="AC1Y77_001871"
     CDS             complement(10378..10938)
                     /locus_tag="AC1Y77_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575435.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11112..12068
                     /locus_tag="AC1Y77_001872"
     CDS             11112..12068
                     /locus_tag="AC1Y77_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1575436.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         3630 a         2653 c         2047 g         3744 t
ORIGIN      
        1 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
       61 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
      121 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
      181 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
      241 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
      301 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
      361 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
      421 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
      481 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
      541 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
      601 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
      661 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
      721 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
      781 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
      841 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
      901 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
      961 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     1021 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     1081 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     1141 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     1201 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     1261 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     1321 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     1381 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     1441 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     1501 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     1561 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     1621 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     1681 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     1741 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     1801 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     1861 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     1921 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     1981 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     2041 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     2101 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     2161 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     2221 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     2281 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     2341 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     2401 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     2461 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     2521 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     2581 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     2641 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     2701 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     2761 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     2821 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     2881 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     2941 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     3001 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     3061 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     3121 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     3181 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     3241 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     3301 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     3361 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     3421 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     3481 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     3541 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     3601 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     3661 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     3721 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     3781 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     3841 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     3901 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     3961 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     4021 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     4081 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     4141 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     4201 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     4261 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     4321 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     4381 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     4441 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     4501 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     4561 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     4621 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     4681 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     4741 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     4801 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     4861 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     4921 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     4981 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     5041 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     5101 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     5161 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     5221 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     5281 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     5341 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     5401 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     5461 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     5521 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     5581 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     5641 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     5701 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     5761 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     5821 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     5881 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     5941 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     6001 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     6061 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     6121 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     6181 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     6241 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     6301 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     6361 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     6421 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     6481 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     6541 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     6601 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     6661 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     6721 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
     6781 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
     6841 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
     6901 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
     6961 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
     7021 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
     7081 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
     7141 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
     7201 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
     7261 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
     7321 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
     7381 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
     7441 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
     7501 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
     7561 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
     7621 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
     7681 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
     7741 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
     7801 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
     7861 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
     7921 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
     7981 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
     8041 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
     8101 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
     8161 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
     8221 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
     8281 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
     8341 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
     8401 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
     8461 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
     8521 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
     8581 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
     8641 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
     8701 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
     8761 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
     8821 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
     8881 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
     8941 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
     9001 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
     9061 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
     9121 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
     9181 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
     9241 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
     9301 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
     9361 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
     9421 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
     9481 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
     9541 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
     9601 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
     9661 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
     9721 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
     9781 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
     9841 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
     9901 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
     9961 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    10021 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    10081 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    10141 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    10201 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    10261 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    10321 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    10381 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    10441 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    10501 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    10561 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    10621 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    10681 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    10741 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    10801 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    10861 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    10921 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    10981 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    11041 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    11101 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    11161 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    11221 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    11281 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    11341 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    11401 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    11461 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    11521 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    11581 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    11641 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    11701 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    11761 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    11821 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    11881 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    11941 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    12001 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    12061 atatgtaatt ttaa
//
LOCUS       DBNIBO010000089        11877 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.093, whole genome shotgun sequence.
ACCESSION   DBNIBO010000089 DBNIBO010000000
VERSION     DBNIBO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.093"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(3..2714)
                     /locus_tag="AC1Y77_001873"
     CDS             complement(3..2714)
                     /locus_tag="AC1Y77_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIQ1575437.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(2724..3425)
                     /locus_tag="AC1Y77_001874"
     CDS             complement(2724..3425)
                     /locus_tag="AC1Y77_001874"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575438.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3611..4210
                     /locus_tag="AC1Y77_001875"
     CDS             3611..4210
                     /locus_tag="AC1Y77_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HIQ1575439.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4291..5304
                     /gene="rbsR"
                     /locus_tag="AC1Y77_001876"
     CDS             4291..5304
                     /gene="rbsR"
                     /locus_tag="AC1Y77_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose utilization transcriptional repressor
                     RbsR"
                     /protein_id="HIQ1575440.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5412..6329
                     /gene="rbsK"
                     /locus_tag="AC1Y77_001877"
     CDS             5412..6329
                     /gene="rbsK"
                     /locus_tag="AC1Y77_001877"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIQ1575441.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6357..6752
                     /gene="rbsD"
                     /locus_tag="AC1Y77_001878"
     CDS             6357..6752
                     /gene="rbsD"
                     /locus_tag="AC1Y77_001878"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HIQ1575442.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            6752..8125
                     /locus_tag="AC1Y77_001879"
     CDS             6752..8125
                     /locus_tag="AC1Y77_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1575443.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8482..10626
                     /locus_tag="AC1Y77_001880"
     CDS             8482..10626
                     /locus_tag="AC1Y77_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIQ1575444.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10678..11718)
                     /locus_tag="AC1Y77_001881"
     CDS             complement(10678..11718)
                     /locus_tag="AC1Y77_001881"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIQ1575445.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         3627 a         2400 c         2252 g         3598 t
ORIGIN      
        1 ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta
       61 acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac
      121 catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca
      181 gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt
      241 tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat
      301 aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc
      361 agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct
      421 tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc
      481 agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat
      541 gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga catataaata
      601 aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct
      661 tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc
      721 gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg
      781 ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca
      841 tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt
      901 tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat
      961 gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac agaacttaga
     1021 gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc
     1081 gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag
     1141 ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt
     1201 gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt
     1261 gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca
     1321 taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt
     1381 ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt
     1441 gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc
     1501 ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg
     1561 gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga
     1621 tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta
     1681 gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca
     1741 cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat
     1801 gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc
     1861 gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca
     1921 tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct
     1981 aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt
     2041 ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat
     2101 aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg
     2161 ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg
     2221 acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata
     2281 tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt
     2341 tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa
     2401 aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct
     2461 acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta
     2521 gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc
     2581 atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg
     2641 atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat
     2701 gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca
     2761 tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc
     2821 gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt
     2881 tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca
     2941 cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg
     3001 cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat
     3061 acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt
     3121 gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg
     3181 taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga
     3241 ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca
     3301 cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt
     3361 gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac
     3421 ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt
     3481 ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa
     3541 tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag
     3601 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
     3661 tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc cacacactgc
     3721 agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt
     3781 gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac aaactgatat
     3841 gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga aggattcttt
     3901 gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt tttttggtac
     3961 ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa gcggtgtagc
     4021 gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt accatatcgt
     4081 gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt tcataaaaaa
     4141 gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag ctatccaaca
     4201 aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt catgggaaaa
     4261 taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa
     4321 gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa tggaaatacg
     4381 gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga attaggctat
     4441 cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat tggtgtactc
     4501 gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga ggatatcttg
     4561 tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca aaaagaaatc
     4621 gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc gacttctgct
     4681 gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt cattgttttg
     4741 gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt tcgcggtggt
     4801 tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct tgtctatcct
     4861 gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt
     4921 taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa acaaggtggc
     4981 taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc tttaaatgat
     5041 gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat ccctgaagac
     5101 tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc gaaactgaca
     5161 acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt gttagatcgg
     5221 atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt agaaaaaaga
     5281 ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc gaactattaa
     5341 aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg agatagttgg
     5401 aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac actacccttc
     5461 gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg
     5521 gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca cggacatctt
     5581 ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta ctgacaaaag
     5641 aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga caagcaatga
     5701 ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca aataatgctt
     5761 tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac tttattattg
     5821 ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca aaagacgcag
     5881 gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact gaactacttg
     5941 aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca ggaatcagag
     6001 tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa
     6061 cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa gaacacggta
     6121 tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat acatttattg
     6181 gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg atcctttatg
     6241 gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct atcccttacc
     6301 gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa acaaacatga
     6361 aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg gggcactttg
     6421 ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt
     6481 tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt ttagaagaac
     6541 ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg gaaatattgg
     6601 aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat gaagaaatga
     6661 aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca ccttacgcca
     6721 atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc ctgaacagat
     6781 ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag acaatattga
     6841 ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa
     6901 tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac tcgatagata
     6961 tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga cgcgtcaaat
     7021 cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg gtttgatatc
     7081 ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa aaggtctggt
     7141 tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc atattttgtc
     7201 tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt ctgctgccaa
     7261 aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga ttgaaccgaa
     7321 tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg ggattcctgt
     7381 agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt tcttttattc
     7441 ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag tatttgtacc
     7501 tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc ctgcgggtgt
     7561 ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta acggaccgat
     7621 cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta tccaatgggc
     7681 tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt
     7741 gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa
     7801 acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac ttgcgggagt
     7861 gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg ttgctgcttg
     7921 cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag cgatcgcttt
     7981 tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt tctggtactt
     8041 agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta gcgggtttga
     8101 tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag atgtttatca
     8161 catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt gatactttcc
     8221 aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa
     8281 caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt
     8341 atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg
     8401 acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga
     8461 aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact
     8521 tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata
     8581 gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg
     8641 gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca
     8701 actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt
     8761 acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct
     8821 gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg
     8881 aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat
     8941 ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct
     9001 attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt
     9061 gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac
     9121 aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat
     9181 tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca
     9241 aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat
     9301 acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa
     9361 tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga
     9421 gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt
     9481 gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc
     9541 tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc
     9601 gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg
     9661 aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca
     9721 gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt
     9781 tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa
     9841 aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa
     9901 aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga
     9961 aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa
    10021 ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc
    10081 cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg
    10141 atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca
    10201 aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat
    10261 tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa
    10321 aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc
    10381 gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg
    10441 ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg
    10501 aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact
    10561 gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag
    10621 aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta
    10681 tttctttata tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt
    10741 gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat
    10801 accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc
    10861 tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg
    10921 gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc
    10981 gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt
    11041 aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt
    11101 atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga
    11161 ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt
    11221 gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg
    11281 tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag tctgatagaa
    11341 aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa
    11401 gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta cttcgaacgt
    11461 ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc cgattgaagc
    11521 accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta agatcaactc
    11581 tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac
    11641 agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt
    11701 ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac tgattccagt
    11761 atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa aatttactat
    11821 attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca acctctt
//
LOCUS       DBNIBO010000090        11680 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.094, whole genome shotgun sequence.
ACCESSION   DBNIBO010000090 DBNIBO010000000
VERSION     DBNIBO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.094"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(14..979)
                     /gene="nrdF"
                     /locus_tag="AC1Y77_001882"
     CDS             complement(14..979)
                     /gene="nrdF"
                     /locus_tag="AC1Y77_001882"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HIQ1575446.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1029..3188)
                     /gene="nrdE"
                     /locus_tag="AC1Y77_001883"
     CDS             complement(1029..3188)
                     /gene="nrdE"
                     /locus_tag="AC1Y77_001883"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HIQ1575447.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDRLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3347..3571)
                     /gene="nrdH"
                     /locus_tag="AC1Y77_001884"
     CDS             complement(3347..3571)
                     /gene="nrdH"
                     /locus_tag="AC1Y77_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HIQ1575448.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3972..4445
                     /locus_tag="AC1Y77_001885"
     CDS             3972..4445
                     /locus_tag="AC1Y77_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIQ1575449.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4442..6574
                     /gene="feoB"
                     /locus_tag="AC1Y77_001886"
     CDS             4442..6574
                     /gene="feoB"
                     /locus_tag="AC1Y77_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIQ1575450.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6576..6710
                     /locus_tag="AC1Y77_001887"
     CDS             6576..6710
                     /locus_tag="AC1Y77_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIQ1575451.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6804..7448
                     /locus_tag="AC1Y77_001888"
     CDS             6804..7448
                     /locus_tag="AC1Y77_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1575452.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7635..8828
                     /locus_tag="AC1Y77_001889"
     CDS             7635..8828
                     /locus_tag="AC1Y77_001889"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HIQ1575453.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8821..10548
                     /locus_tag="AC1Y77_001890"
     CDS             8821..10548
                     /locus_tag="AC1Y77_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HIQ1575454.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            10942..11139
                     /locus_tag="AC1Y77_001891"
     CDS             10942..11139
                     /locus_tag="AC1Y77_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HIQ1575455.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            11141..11452
                     /locus_tag="AC1Y77_001892"
     CDS             11141..11452
                     /locus_tag="AC1Y77_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HIQ1575456.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            11556..>11680
                     /locus_tag="AC1Y77_001893"
     CDS             11556..>11680
                     /locus_tag="AC1Y77_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HIQ1575457.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT         3726 a         2018 c         2413 g         3523 t
ORIGIN      
        1 ttaaaataaa tccttattcc aatccaatca aataatcgtc gtctttcatc gcttctacat
       61 gacctaacaa atagccgttt ccgacttgtg agaagaagtc atggttgctt gtgcctgttg
      121 aaatcccgtt cattacgatt gggttgacat cattggcagt atctggaaag agtggatcca
      181 tccctaggtt catcaatgct ttgttggcat tatagcgtag gaatgtcttg acttcttctg
      241 tccagccgat tggatcgtat aattcttctg tataacgttc ttcgttttcg taaagctcat
      301 aaagaagatt gtacatccag tcttttaacg tttcttgttc tgcttcaggt aactcgttga
      361 atcctaattg gaatttgtaa ccgatgtagg ttccatgtac agattcatct cggatgatta
      421 gtttgatgat ctctgctacg ttagccaatt tgttgtttcc taaataataa agaggagtat
      481 aaaaacctga atagaacaag aatgtttcta aaaagacgct ggcaattttc ttttctagcg
      541 gtgtaccgtg tttgtagatt tcattgatac gctcagcttt tttttgcaga taaggattcg
      601 tatttgtcca gtcaaaaatt tcttcgattt cttttttcgt attcagtgta ctaaaaatag
      661 aagaataact tttggcatga acggattcca tgaattgaat attgttcaaa acggcttctt
      721 catggggagt gcggacatct ttacgcaact gatccatgcc actttcagat tggacagtat
      781 ctagcagtgt caaaccgcca aatacatgtc ctactgtatc tttttccaat tgagacaatg
      841 tacgccaatc atctaggtca tttgaaagtg ggatacgtgt atctagccaa aattgctctg
      901 tcagcttctc ccaagtggat ttatcaatga tatcttcaat ttcattccag tttattgctt
      961 cataatatgt ttctgacatg agaatgctcc tttaattaaa atatgaataa gcgaatctat
     1021 ctatgatatt agatcacgca gctttcacat tgattgcttc cgatttcttc tgcatcatca
     1081 gtaaaggtac gtacgtaata aatggatttg attcctttgt ggaaagcgta atgtcttaaa
     1141 atattcaaat cacgtgtcgt ttgcttcgtt gttgtcttcc attcataaat gccttctggg
     1201 atttcagaac gcatgaataa agtcaaactc atgccttgat cgacgtgttg ttgtgccgtt
     1261 gcataaacat cgattacttt acgcatgtcc atatcataag cagaagtgta atacggaatc
     1321 gtctcattgg atagataagg cgctgggtaa taaattttac cgattttctt ctcttggcgt
     1381 tcttcgatca tgcgagtgat aggatgaata cttgcactcg tatcgttgat atatgaaatt
     1441 gagccatttg gtgctactgc caagcgattt tgatgataca gtccatcttt cattactgct
     1501 tgtttcaaag cattccaatc ttcagcagtt gggataaaaa tgccttcaaa caatgctttg
     1561 actttttctg attttggctg gaattctcca gttacgtatt tttcgaaata agtgccgtca
     1621 gcataagctg atttttcaaa attatggaaa acttgttttc tttcacgagc gatattgttg
     1681 ctttcaacaa gtgtccaata attcaacagc atgaagtaaa tatttgtaaa gtctaatgaa
     1741 tcctctgagc catattccat ttgttctttt gcaagataag tgtgtaagcc catggcacct
     1801 aatccaatcg tatgattcag cttatttccg ttttggatcg gtggtacgac atcgatatca
     1861 gatgcatctg taataaatgt caatgcacga gtcatcgtac gcacagattg accaaagtta
     1921 gggctttgca tcaaattcac gatatttgtc gagcctaagt tacatgagat atctgttcct
     1981 aatacttcat attcctgttt gccgttaatc acagaaggcg tttgaacttg taaaatctct
     2041 gaacataaat tactcatgat gatcttgcca tcgattggat tctcgcggtt cgctgtatca
     2101 atattgatta cgtaaggata gccagattct tgttgtaatt ttgagatttc attttcaaga
     2161 tcacgcgctt tgattttttg tttgcgaatg tttggatttg caactagacg atcatactca
     2221 gctgtaatat ttacataaga gaatgggaca ccatattctt tttcgacact atatggacta
     2281 aataaataca tatcttcgtt tttacgtgtc aattcataaa atttatcagg tacgatcacg
     2341 ccaagagaaa gagtttttac gcgaatcttt tcatctgcat tttctttttt tgctgaaagg
     2401 aaagcaataa tgtctggatg gaaaacgttc aaataaacca cgccagctcc ttgacgctga
     2461 cctaattgat tagaatagct gaaactatct tcgaataatt tcatgactgg aacgacacca
     2521 cttgcagcac cctcatatcc tttgatagga gcgccggctt cacgaagatt tgataaagta
     2581 atccctacgc cgccaccaat acgggacaat tgcaaagcag agttgattga gcgcccgata
     2641 gagttcatat cgtcagtgac ttgagtcaag aaacaagaca ccaattcgcc ccgacgttta
     2701 cgtccagcat tcaagaaaga aggtgtagct ggctgatatc gttgatggat catttcgtct
     2761 gcaagagcaa gtgctaattc ttcatccccg ttcgcaaagt aaagcgcatt gaaggctact
     2821 cgatcttcat aagtttccag ataagcggaa ccgtcgttgg ttttcaacgc atattgagaa
     2881 taaaatttgt aagcagccat aaaagatttg aaagaaaaat gttgctcgtc caaaaattga
     2941 tatagctttt caataaatga tggtgaatac aattgaatga attctgcttc tatatattct
     3001 tgatcgatca agtaattgat tttttccata atagatggaa aagtcatcgt atttggtttg
     3061 acattttcct caaaaaaggc aactaaagct tctttatcct tattcaaagg gatttgacca
     3121 ttgactggcc gattgatttc attattcagc ttgaaataac tgacatcttg taaattttta
     3181 agactcatac gtacgcccaa ccttcatttc gtatttgatg atgcttgatt gcaaatcatt
     3241 gcattgtttt taagctttaa aaaaggattt ggaagtttgc gacaaaaact ggtgctgtct
     3301 caagcccttc aaatccatgt atcacgtttt gattctttta gtcatgttag ctggcaagtt
     3361 tacgcaactg atcaggacgg aatccgataa ctgtttctgc atcagaagta atgacaggaa
     3421 cactttggaa tccttgtgct ttcaaccagt ctactgcttc aggctgttca tcgatattga
     3481 cttcttcaaa agcaatttga ttttctgtta aaaaccgttt gaccatctta cattgcatac
     3541 aattattttt agaaaagatt tttacgttca tggtgagtgc ctcctctaac acattcttat
     3601 tatctataat agatagtata aaactcttag taaaaaagtc aaacaacaaa cactacatat
     3661 tgtgttgtgg aaactgtgga atactaagtt ttgtgttttt gaaaaaagag ctgaaaacaa
     3721 gtatcgtgtg ggaaaatagc aaatttaata gagaaaaaat tgtggaaaac tatgaagaaa
     3781 tatttttttc tcatttttat ctatcaatac taaagattcg ctcataatat cacaagtata
     3841 attgacattc tcaattaccg agagtattct atgtgagaat gattctcatt tatttgcgta
     3901 acacgtaaga aaattagaac atataatgaa tgattttaat acatgattta tttcacaaga
     3961 gaggaatcaa tatgcagacc ttagcgcagg caaagcagaa tttcgtttat caagtcgaac
     4021 gaatcgaaac agaagaaaaa acaaaacgcc atttgcaaaa tatcgggatc gttgccggaa
     4081 gtaaaatcgt cgtcgtcaat ttatcaggca acaatgccat tttactggta aaaagtcatc
     4141 gagtggcagt aagtcgggaa atactaggtc aaatctttgt caataaaaag actgcttcct
     4201 ctgaaagctg gacttctctt gatcaattaa aagcaggaga gactgggatc gtcgtagcaa
     4261 tccacggaca aggcgcagtg aagcgacggt taatggatat gggattgacg cgaaatgtaa
     4321 aaatagttat ccgaaaacta gcccctctgg gagatccaat cgaactgaca gtgcgagggt
     4381 atgaactgac gttacgaaag tcagaggccg aactaatctt gatacaaagg gaggaaaaag
     4441 aatgaatatc gcgttagtcg gaaatccaaa cagcgggaaa actagcttgt tcaatctctt
     4501 gactggttcg aatcaatatg tagggaattg gccgggcgta acagtagaaa aaaaagaagg
     4561 atcgttgaaa aaaaatccga acattcatat tcaggaccta cccggcatct attctttgtc
     4621 tccttataca ccagaagaag tgattgcaag agattatttg gttcaggaac agcctgaact
     4681 gatcatcaac attattgatg ctaccaattt agaacgaaat ctttatttaa ctacgcagtt
     4741 attggattta gacattccta tgatcattgg tctgaatatg atggatgtca tcaaaaaaga
     4801 aggaaaaaaa atcaatctag aaaagctttc ctatggtctt ggtgtaccag ttttaccaat
     4861 cagcgtattg aaaaagcaag gaatcgaaaa actaatcgaa aagattcagg agtctgcaaa
     4921 aaagcggcct tctattcctg ttccctgtac atatgatcca cgtttagaag cagctttaca
     4981 tgaaattgaa gatgtactag gagaacaaag cggcaagaaa cgctggtatg caatgaaata
     5041 ttttgaacga gatcagaagg taagagagga ttacgagata gcagtttctc agcaaaaaga
     5101 gatcgagcaa ctgatccagt taacagagaa actattggac gacgatagtg agacgattct
     5161 ggtcaatgaa cgttatgaat ttattaccca actatgtaca ctctgtgttg tgtcaaatga
     5221 ttctttccaa ttatcgatga gtgacaaaat cgatcagatt gcaacgaatc gttggttagc
     5281 cttgccgatc ttcgcatttg tgatgtggct gatctattat ctggctattc aaacagtagg
     5341 aacaatgggg acagattgga tcaatgacac attgtttggt acatggttac cagaacatgt
     5401 tggcagattg ttagctgaat ggcaagtagc gggatggatg caggagctga tcttgaacgg
     5461 catcatcgca ggcgtgggag cagtgttagg atttctaccg caattgatta tattgttttt
     5521 atgcttgagc tttctggaag actgcggata tatggcgagg atcgcttttg tcatggatcg
     5581 acttttcagg aaattcggtc tttcaggaaa atccttcatt cctatgctga ttgctacagg
     5641 atgtggagtg ccaggcgtca tggccagtcg cacgattgaa aacgagcaag atcgtcgttt
     5701 gacgatcatg gtcacaacct tcatgccttg ttctgcaaag ttgccgatta ttgccttagt
     5761 agcaggggcc ttttttcctc atcaatcttg ggtagctcct tctgcctatt ttcttggtat
     5821 gactgcaatt attttctctg gaatcagctt gaaaaaaaca cgtttgtttg ctggagatac
     5881 agctcctttt atcatggaat tgcccgcata tcattttcct caatggtcag cggtcctaag
     5941 acaaacctat gaccgcagca agtcttttgt taaaaaagca ggaacgatta tctttgtttc
     6001 tagtatcgtg atctggttca gctcttctta taatttttat gctcaacctg tttctgaaga
     6061 ccaaagtatc ttagcctttt tcgggcgaat cctggcagtt gtttttcagc cgcttggttg
     6121 gggaaactgg caaggaacag tcgcagcgat tacaggattg gtcgctaaag aaaatattat
     6181 tggcacattt ggtattttgt ttggtcatgc ggaagtatcc gaaaatggca gagaaatctg
     6241 gcaagtgcta caacacacat atactcccgc agcagcttat tcattgttag ccttcaattt
     6301 actttgtgcg ccttgttttg cggcaattgg ggctatccat cgggaaatga atgccaagaa
     6361 atggacttgg atcgccatag gctatcaatg cggtttagct tatttagtta gttttgtgat
     6421 ttatcagttg ggacacttat tggagggtgg acaggtagcg ttagggacgt atcttgctat
     6481 tttactgatg ataggactcg tttatgtttt gttccgtcag ccaaaatcca agaaccagtt
     6541 ttatactata ttgtcattag aaggagagga ataggatggc gacattctta ttaagtgtgt
     6601 taatctttgg aagtgcaggc gcaattgtct atactcgttt aaaatccggt aagaattgtg
     6661 atgactgtca aacagcttgc ccagtaaaaa aggaacagat caagcgatga tcagtcgttg
     6721 caagaggcta ttctcccctt ttttgcttat tttgctagag catagtaaga tgagggaaag
     6781 taaaaaaaga aagaggaaag aaaatgtctg gaaagaaaaa aatcgtccgc ccgcgctacc
     6841 agcagattgc tgttgattta gccgaacgta tcgttgaaaa ccgatataaa gtgggtgaga
     6901 agatccatgc ccgctcaaca cttgccagta cgtacaatgc ttctcctgag actacccgca
     6961 aggctatcaa tgtgctggta gatcttggaa tcatggaagt acggcatgga agtggggcat
     7021 ttgttgcttc gaaggaaaaa gcaaaagatt tcctattgca atatcaagat gtacaatcca
     7081 tccaagatac aaaagcagaa atcatggcaa gtgtggagaa gcaacaagat gagctgaatc
     7141 atttttctca attattggat cagctggtca atcaaacgaa gcgaatacat aagatgaatc
     7201 ctttacttcc gtttgaatta tctttatcag aaaaagcaat gcatctggac cgttcaatta
     7261 gcgagttgaa tatctggcag gcgactggcg ccacagttat cgcaatttct cataatgaag
     7321 aactgcttgt ttcacctggc ccctatgcta aatttacggc aggggacatc gttcatttta
     7381 ttggaaatga atatacacta cagcgtatga caaacttttt ttacccggag catcagcttg
     7441 cagaatgatt gtgtgaaata ttaaaaaaat attaacttgg ctaaaccttt tatttacaaa
     7501 gatttagcca agtttttttc agtttggaca agcaaatttc tttcattttt gacaaagtga
     7561 ctacatgatg ttaacatagt gtggtcactt ttttcagaaa gaaaaatgaa tttacataaa
     7621 aaaggagaat ttgtttggta aaagtagaag taaaacattt aacaaagata tttggtaaaa
     7681 agacacaagc cgctttagat atgatgaatg atcaccagcc gaaaacggag attttaaaga
     7741 aaaccggtgc aacggttggt gtgtatgacg tgaattttga cgtaaaagaa ggagaaatct
     7801 tcgtgatcat ggggctttcc gggagtggga aatccacatt gatccgtcta ttgaatcgtc
     7861 tgattgagcc aacttcgggc agtatctata ttgatggaga agatgtatcg aagttaagca
     7921 aagaagaatt gcgagaagtc cgtcgtcata aaatcaatat ggttttccaa aacttcggtc
     7981 tgtttccaca tcgaacgatc ttggaaaata cagaatacgg gttagaagtc cgtggtgtac
     8041 ctaaagaaga acgacaagaa aaagcagaaa aagcattaga aaattctagt ttgctttcat
     8101 ttaaagacca atatcctagt cagttatctg gtgggatgca acaacgggtt ggcctagcca
     8161 gagctttagc aaatgatcct gagatcttgc tgatggacga agcgttctcg gctcttgatc
     8221 cgctgatccg gcgagaaatg caagacgaat tactggacct gcaagccaat gttcaaaaaa
     8281 cgattatttt cattacccat gatttgaatg aagctttacg gattggggac agaatcgcct
     8341 tgatgaaaga cggcgaaatc atgcagatcg gaacaggtga agaaatattg accaatccag
     8401 caaatgatta tgtacgtgaa tttgtggaag aagtcgatcg ttccaaagtg cttacggctc
     8461 aaaacatcat ggtacctgca ttgacgacga atatcgaatc tgatggacca aacgttgcat
     8521 tgacaaggat gcgtaatgaa gaagtcagca tgctgatggc tgttgacaga aagagacacc
     8581 tgaaagggat catcactgca gatcaagcgt tagaagctag gaagcaaaaa cgtccattga
     8641 tcgacttcct agatgaaaat gtgacggtga ttgggaaaga catggtggta agtgatattt
     8701 tcaatattat ttatgactca ccgactccac ttgcagttgt agaggatgga aaattaaaag
     8761 gtgtcgtcat ccgaggtagt gtgattgaag cattagcaga aacaagcgag gtgagtgaac
     8821 atgaataatc tattgaacta tcaattacca gtggcaaact gggtagaaaa tatcacagaa
     8881 tggtttacga caactttttc tggcctattt tcatttttac aaacgattgg acaggcagtg
     8941 atgtcgggga ttacaaattt actgttagtg attccagctc ctttgtttat cttgttattg
     9001 acggtagcag ctttctttat ttctaagaaa cgtccaggac ttacgttgtt cacattaatc
     9061 ggtctatggt ttatttataa ccaaggtcta tggaatgatt tgatgaatac tgtcacactt
     9121 gttcttttat ctagtgtgat ctcgatcatc attggggtac cattagggat cttgatggca
     9181 aaaagcagta aagcccaaag tattatcaaa ccgatccttg actttatgca gacaatgcca
     9241 ggattcgttt atttgattcc agcagttgca tttttcggta tcgggatggt tccaggggta
     9301 tttgcctcag tcatctttgc tctgccacca accgttcgtt ttacgaattt agggattcga
     9361 caagttccta aagaattagt agaagcttct gattcattcg ggagcacaag tcgtcaaaaa
     9421 ctattcaaac tagaattgcc tttagctaaa agtacgatca tggcagggat caatcaaaca
     9481 acaatgctgt cactttcaat ggtcgttatt gcctctatga tcggtgcacc cggcttagga
     9541 cgcggcgtat tatcagcatt gcaaagagca caagtcggga atggatttgt caacggggtt
     9601 gcattagtta tcttagcaat catcattgac cgttttacac agcatttgaa ccagccaaac
     9661 aataaaaaag ctgcaggagc aacaacacag aaaagtaaaa aacgtcaagg actgatcatt
     9721 ggtgctgtag tagtagtgat cttaggtgcg attggtatag ggagtttttc atctgccaaa
     9781 gaaacaaaac gtatcaactt atcttatgtt gaatgggata cagaagttgc ttcaacaaat
     9841 gtagtcggtg aagtattgaa acaaatgggc tatgatgtaa cgatgacgcc attagataat
     9901 tcaatcatgt ggaaatctgt ttcaaatggc gagtcagatg ccatggtttc agcttggcta
     9961 cctaaaactc atggttcaca atatgcacaa tataaagatc aagtcgaaga cctaggtgcc
    10021 aatttgacag gagcaaaagt tggtttagca gtgccagcct acatggatgt aaattcgatc
    10081 gatgagttaa ccgatcaggc aggtaagaaa atcataggaa tcgagccagg agctggtgta
    10141 gttacggctg ctgaaaacac gatccaaaaa tatgacaact tgaaagactg gaaagtagag
    10201 acatcttctt caggagcaat gaccgttgca ctaggtcaag cgatcaaaaa acacgaacca
    10261 attgtagtta caggatggac acctcattgg atgtttgcca aatatgatct gaaatactta
    10321 gaagatcctg aaaacggaat gggaagcgaa gagcagatcc atacgatggt tcgaaaagga
    10381 ttgaaagaag accagccaga agcttacaaa gtattagaca atttccattg gtcagaaaaa
    10441 gatatggaaa aagttatgtt ggaaatcaac aatggaaaag atccacagca agcagcaaaa
    10501 gactggatca aagagaacca agaattagtc gaaagctgga aaaaataaaa agacagacac
    10561 gaaataaatg gagcttccaa aattggatca aatatccaaa tttggaagct ccatgttcgt
    10621 ctctatcttt tttgtctata aaaaaagaga atgcgtagct catcttcgaa acgttcaaag
    10681 acgccgacaa actattatga atttatgggt gcgagtcaaa gagatcgatc gtaggtatga
    10741 aagactaatg tgattaaagt ttattatgta cgaagtgcat tctcaaaaaa tattaaagaa
    10801 tatacatagt ttatatactt aaaagtgatt aaattcttat tcaggaatta ttttgctacg
    10861 tttcaagaag gaattgtttt ttgaaattta atgaatgtta gaataatagt ctaaaaacat
    10921 taatttaggg gtgattagat tatgaaacat ttaaaaattt tgtctattaa agagacacaa
    10981 cttatctatg ggggtaccac tcatagtgga aaatattatg gaaatggagt gtattgcact
    11041 aaaaataaat gtacggtcga ttgggccaag gcaactactt gtattgcagg aatgtctata
    11101 ggtggttttt taggtggagc aattccaggg aagtgctaaa atgaaaaaaa atgctaagca
    11161 aattgttcat gaattatata atgatatatc tataagtaaa gatcctaaat attctgatat
    11221 tcttgaggtt ttacaaaagg tatatttaaa attagaaaaa caaaaatatg aattagatcc
    11281 cagtccttta ataaatagat tggtgaatta tctatatttt actgcttata ctaataaaat
    11341 aagattcact gaatatcaag aggaattaat aagaaatttg agtgaaattg gaagaactgc
    11401 tggaataaat ggtttatatc gagcagatta tggagataaa tctcaatttt aatgatactt
    11461 ataatattaa atatgtctca aaattttttg attagaaaaa taggtatatt gaatcagtga
    11521 cataaaagta tttatagttt gtagggggaa tcgtaatgga agaaaaaaat agactaaatg
    11581 ctaaacaatg ttcagatcaa gagcttaaaa aaattaaagg aggtgctgga acaaaacctc
    11641 aaggaaaacc ggcatcaaat ttagtggaat gtgttttttc
//
LOCUS       DBNIBO010000091        11591 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.095, whole genome shotgun sequence.
ACCESSION   DBNIBO010000091 DBNIBO010000000
VERSION     DBNIBO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.095"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            107..970
                     /locus_tag="AC1Y77_001894"
     CDS             107..970
                     /locus_tag="AC1Y77_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIQ1575458.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="AC1Y77_001895"
     CDS             complement(1099..2721)
                     /locus_tag="AC1Y77_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1575459.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="AC1Y77_001896"
     CDS             2970..3491
                     /locus_tag="AC1Y77_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIQ1575460.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="AC1Y77_001897"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="AC1Y77_001897"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HIQ1575461.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="AC1Y77_001898"
     CDS             5572..6390
                     /locus_tag="AC1Y77_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIQ1575462.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="AC1Y77_001899"
     CDS             complement(6493..6690)
                     /locus_tag="AC1Y77_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575463.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="AC1Y77_001900"
     CDS             6859..7437
                     /locus_tag="AC1Y77_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIQ1575464.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7532..7669
                     /locus_tag="AC1Y77_001901"
     CDS             7532..7669
                     /locus_tag="AC1Y77_001901"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575465.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8482..8985)
                     /locus_tag="AC1Y77_001902"
     CDS             complement(8482..8985)
                     /locus_tag="AC1Y77_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1575466.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="AC1Y77_001903"
     CDS             9344..10369
                     /locus_tag="AC1Y77_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIQ1575467.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10692..11570
                     /locus_tag="AC1Y77_001904"
     CDS             10692..11570
                     /locus_tag="AC1Y77_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HIQ1575468.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3587 a         1973 c         2259 g         3772 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttgaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10561 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10621 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10681 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10741 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10801 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10861 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    10921 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    10981 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11041 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11101 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11161 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11221 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11281 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11341 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11401 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11461 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11521 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11581 tcgaaatagt t
//
LOCUS       DBNIBO010000092        11570 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.096, whole genome shotgun sequence.
ACCESSION   DBNIBO010000092 DBNIBO010000000
VERSION     DBNIBO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.096"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(157..2055)
                     /locus_tag="AC1Y77_001905"
     CDS             complement(157..2055)
                     /locus_tag="AC1Y77_001905"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIQ1575469.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2218..3480)
                     /locus_tag="AC1Y77_001906"
     CDS             complement(2218..3480)
                     /locus_tag="AC1Y77_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575470.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(3473..4372)
                     /locus_tag="AC1Y77_001907"
     CDS             complement(3473..4372)
                     /locus_tag="AC1Y77_001907"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575471.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(4517..6295)
                     /gene="uvrC"
                     /locus_tag="AC1Y77_001908"
     CDS             complement(4517..6295)
                     /gene="uvrC"
                     /locus_tag="AC1Y77_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIQ1575472.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(6477..6791)
                     /gene="trxA"
                     /locus_tag="AC1Y77_001909"
     CDS             complement(6477..6791)
                     /gene="trxA"
                     /locus_tag="AC1Y77_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIQ1575473.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6878..9238)
                     /locus_tag="AC1Y77_001910"
     CDS             complement(6878..9238)
                     /locus_tag="AC1Y77_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIQ1575474.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(9353..9900)
                     /locus_tag="AC1Y77_001911"
                     /pseudo
     CDS             complement(9353..9900)
                     /locus_tag="AC1Y77_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(9958..10395)
                     /gene="zapA"
                     /locus_tag="AC1Y77_001912"
     CDS             complement(9958..10395)
                     /gene="zapA"
                     /locus_tag="AC1Y77_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIQ1575475.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            10597..11514
                     /gene="rnhC"
                     /locus_tag="AC1Y77_001913"
     CDS             10597..11514
                     /gene="rnhC"
                     /locus_tag="AC1Y77_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIQ1575476.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
BASE COUNT         3268 a         2455 c         1964 g         3883 t
ORIGIN      
        1 gtgaaatcag cgccctgacc tgagtattag agaaataaac agaaaaatag cttctaatat
       61 tttaccgttt tttgagctaa ttccgaagga gactgcgaca cgccttttgt tgcagtcgct
      121 ttttagtcta ttgagcaatg ttggctagca tttttctcaa tcgtcctttc gataatccaa
      181 cagcctttgg agttgagtaa tctgttgctg taattccatt ccttttgtcg tattccgatt
      241 aagtttgcgt gggagctgct gctcgatcac tttttttaca ttcgtttgat cagatttttt
      301 tgcaaattgt tgttcaatag gcaagaacgg ataatgtgtc acgatttgga aaccgtaaga
      361 attgcatagt aatgagtagc cagcaatccc ggttgttttt tgataagctt tggatagacc
      421 tccgtcaata acaaatgctt ttccgtttgc tttgatcggt gactctcctt tttttacttt
      481 tactggcgtg tgtccattga tgattgctga accttcttct tttgcatcga attctgccaa
      541 aattttttca ctgaaccact cttcatctcg caagtgataa tagggattgc ttttttcgac
      601 gtgcgtatga ggctctgtga gaaaatagcg ctcaaaagtt gccattttac tcttgccgaa
      661 taaaggcgaa aatggcccgc tccacgcata ccaaatcaaa tcggtcgcca aatcttcatt
      721 tgcagtcaaa tcttttgcgc tttcagttat ctgtttttca aaaaatccca gcagttcttt
      781 tccgcgataa gattggtcta attcaaatgc taagagctgc cccttttcat ctacaggtat
      841 acagccatgg aacagtacat gctgattgtt cgtgagatac attccccctt tttcgatcaa
      901 aaacgtcatg tgtttttgca ttcttaacga gtgctggaat gaatacatca aactatcgat
      961 cactttttgt tcctcttcca agaatttttt aggatcatct ggctggatcg tttgaaaaca
     1021 agtccctttc aactcataga gctgaccttc taaaacgatc gttgattcct catagttgat
     1081 tttttccaac aatttacgat cgtccatttt aaattcaggt cgtcgattca acaactgatg
     1141 ttctgatttg aattggagga tagaaagtgc ttgatggatc ttttctaatt cttcttgctc
     1201 tctttcagat agatctttcc tgtttttagg ggtaaatact ggattcttct gataggtttg
     1261 atgggcaaaa gaaaataatg gacgtaaatt taatccatat tctttctcta attcaaataa
     1321 atagttgtag cgtgcggcaa tccgtaaaag cgtcaataaa catgcctcag agccgcaata
     1381 cgcaccaatc cacaaaatat catgattccc ccactggata tctacagagg aaaaatccat
     1441 gattcgatcc attactttgg ctgcttgagt tcctcgatca aaaatatcac ccacgatatg
     1501 caaatgatcg atgacagatt tttggatcgt ctgcgcaagt tttgttaaaa atttttcttc
     1561 ttccttcatc aagacaagtt gttcaatgat tttttttaca tagtcttttt tcccgttgat
     1621 acgatcatcg atatacaaga gttcctcaat gatatagcta tattctttcg gcaatccttt
     1681 tcgaactttt gatcgagtgt atttgactgt cgtaaaccgt aaaagatcga tcaaacgatg
     1741 gatcagctgg gatttatctt gttttttgta ccaatcagtc gtcaacacat attctggata
     1801 agccgtaaaa atagtgaatt ggctgatctc cgtttccgtc atttgttcag agaaaaggtc
     1861 tttgatcttt tctttaagat ttcctgaacc gatacgcaga atatgatcaa aagcaggaaa
     1921 ttcgccatgt atatcgctca aaaataactc tgttcctttt ggaagctgca aaattgcttc
     1981 gagattgatg atttccgtga tgacatcttc cgttgtaggg aacttctctg aaagtaaatc
     2041 taaatagcgg tccattaaaa cgcctccaaa aattggtata ctcaacatta taaagttgtt
     2101 atgctctatt ttcaatgaat atatatatcc aatataataa aaaaatagaa ataggtctat
     2161 acaaaaaaga agctgggaac aataaatgtc cagcttcctt ctatattttt ctatatttta
     2221 tgcttttttt cgttcatttc tcataattga aatcgattgt ttcaatgaac tccataatcc
     2281 gccttcgcta tacacgagta cattcgattt gtacatgttt gcagacaaga tcatcaataa
     2341 gaatgttacg gctacaagta ccaacaagga aatcaatgct ccacccatac cgactgtctc
     2401 atttgctaag cgaaccggca taatatatga agataagaat ggaatatacg aagtgatccg
     2461 aataacgata ttgtttggat ctgaagctcc tagtatcaat ccaagcatat atccgcctag
     2521 agatagataa gtcactggca aaattgcttt tgctgtatct tctgctttgt tgaccaaaga
     2581 gccacataaa gctgctaaaa cagaaaaaat caaaatgcca aagatcatga atagaagaga
     2641 ataccacaag aatggtccaa tcaagttatc caatgagata ccgtctaata tatttttgac
     2701 catttccata tctttgaatt gactgtatcc tataccaaaa ataataccgt ataatgccat
     2761 ttgcgttaat gcaacaagta aaacacctgt tagttttccg taaaaatgct tttgtgcagt
     2821 cgtactggac aggatcactt ccatgatccg cgtgcctttt tcagaagcga tctcttgtgc
     2881 aatgatctgc gcatacgtca aaataataat gaacaagaca atcgttgcta cataacttac
     2941 gacatattgg actgtcgagt tatcttctcc aactgtcatt tttccttttg aatcaaaatt
     3001 gactttttgt cgagtcaaac ttgcttgttg gctcaagcta gctacttctt ctggtgaaat
     3061 gcctaattga cttgctcgaa gcatcgattg caagccagtc agttgttgtt gtatcagtag
     3121 ttgtgtactt tgtcctaatg aattttcgct ataaagcatc ccatttatct tatcattctc
     3181 agttgagact actaaatacg catcgatttt ttcttccgaa agctcttttt gtgctttttc
     3241 ttcagaatca actgctttga actcaaaatc atctgttttg acttggctca tttgctgact
     3301 gatttgctca ttatcagcca atacgccgat tttattgaca tcactatttt gttgagcaaa
     3361 gtttcctgct acataaatga tacccagcat gataaaaggg actaatatca tgatcaagaa
     3421 agaaatcgac ttaacatttt ttaaatagac atctttggta ataatccaaa acttacgcat
     3481 cgatttcacc tgctttcaat ttgaaaatct cttccaaagt tggcggttgc tggttaaaca
     3541 taggaatgta gccgaactga gtcgcacgag taaagatttc tttcccagct tcaggatctt
     3601 ctaaggtaat ctctaacgta ttgtcttcat gaaggatcac ttttttcacg ccttcgattt
     3661 cttctacttc ctgttggctt aaaccagatt ccaagaataa cttcgtacgg ccaaatgatt
     3721 cacgaatctc atgtactttt ccattcaaaa ccatttttcc gtttcgcaac atgatcaaat
     3781 gatcgcagat tttttctaca ttatccatat tgtggctaga aaaaatgaca catgaaccat
     3841 gttttttcaa ttccaatatt ccatctttta gcaattcagc atttaccgga tctaacccgc
     3901 tgaatggctc atccaaaatg atcaatttag gttcatggat taacgtggcg atcagctgga
     3961 ctttttgctg atttccttta gacaaagatt ttactttatc cgtctttttc ccttttacct
     4021 gaaatttttc catccaaaaa tcgatttttg gttcaatctc ttttcttgat ttccctctaa
     4081 gttctgcaaa ataaagcagt tggtcttgaa tcgttacttt cggataaagt ccccgttcct
     4141 ccggaagata accgatgaca tcataatctt tctccccaag atgatggcca ttccataaca
     4201 cttctccttg gtcttgtgtc aaaaaatcca agatcaaacg aaaagtcgtt gtcttcccag
     4261 ccccgttttg gccaattagc cccatgattt ttccatcggg gatttggaaa gagacatgat
     4321 cgacagctgt gtagttccca aaagttttaa caagatcctt gacttctaac atttcttcac
     4381 tccttcttca agaaatcaca tatatcctta gacaccagac gtaagcgatt tattatttac
     4441 caacaaaacg taaacgctct ttttgttgta cataaaatga taggacactt tactttcttt
     4501 gtaaagtgcc ctttcctcat tcctgatgta atttatcaaa tacgttttgt gcgacatttt
     4561 tcggcagtcc gatttcttgt aattcttctg tcgtggctgc tgtgatattt ttcaacgatt
     4621 tgaactcttt aagcagcatt ttttttcgtt ttggtcctaa cccttcaatt tcatctaatc
     4681 gggaagcaaa actgttttta ctgcgtagct gccgatggaa agtgattgcg aatcgatgga
     4741 cttcatcttg gattcgctgg agtaagaaaa actctggtga atttcgctca agcggtacta
     4801 cagccaaatc tataccgaat agcaactcat ttgttttatg tttatcgttt tttgccatgc
     4861 cagcaatagg aatatcgatt cctaattgat tttctaagac atcttttgcc acatctactt
     4921 gtccttttcc tccatcgatc aagatcaaat caggtaaagg caacccttcg cgaatcactc
     4981 gtgaatatct gcgatagatg acttctctca ttgatgcata atcatccggt ccgttgactg
     5041 tttttatttt atatttccga tattcttttc ttgccggttt tccatcaatg aatacgacca
     5101 tcgcagccac aggattcgtc cccatgatat tagagttatc aaaagactcg atacgtattg
     5161 gggtcgggat attcatcgcg tttcctaaac gttcaactgc tccgatcgtc cgttcctgtt
     5221 ttctggcaat cagatcaaac cgttcattca aagcaactga ggcattctta ttcgccaatt
     5281 caactaattt tttcttttct cctcgttttg gttgcagcac tttcgtagct aacaaagctt
     5341 ctacactggg cttgtcgatc gtatcaggaa tcaagacttc tttaggaatg aaatgctcat
     5401 tttcctgata aaactgaccg aggtaagtca agaaatcttc ttctgcttcg ttatagaaag
     5461 gaaacattga aacatctcgt tcaattagct ttccttgtct tacgaaaaac acttggacac
     5521 acatccatcc tttgtcaatc gcataaccaa atacatctcg atccagtaaa tcagtatttg
     5581 tcattttttg gcgagtcatg attgtctcaa tggcacggat ctggtctcgg tattctgcag
     5641 ctttttcaaa ctccatgttt tcagcagctt tttccatttt ctgatggatc tctgactcga
     5701 ttttttcgtg tcctccgttt aaaaactttt tcacttcatc aacgattttc gtatagactt
     5761 tcggatctac gtcgaaataa taaggacaca gacattgtcc caaatgatag taaagacaag
     5821 gctccttcgt ctgactaggt ttgcattttc tcagaggaaa taatcgatcg aggatcttct
     5881 ttgtctcatt cgccgcaccg acatcgggat atggtccaaa atacagtgct ttgtctttta
     5941 gtactttacg agtaatcacc aaacggggat atttttcatt cgttatctta aggaaaggat
     6001 aggttttatc atcctttaac ataatgttgt atttaggatc atttttcttt atcagattga
     6061 tctctaagag caatgcttcg atattcgatt ctgtaacgat gtattcaaag tcttcgattt
     6121 cactaacaag ccgttccgtt ttcgtattat ggctgccagt aaaatatgag cggacacgat
     6181 tttttaatat tttggctttg ccgacataaa tgatcgttcc atttttatct ttcatcaagt
     6241 aacagccggg ctgatcagga agtaaagcta atttgttttt gattcgttcg ttcatctggt
     6301 actccccttc atattcaatt aaagttatta tagcatgtct gtcaaaaaaa caatccagcc
     6361 tgacaacgta attatactca aaaaatatta atttgaataa ccttaactca accgcaaaaa
     6421 aaccgaggac ggaactggat tcagtcccac cctcagctta tttttgctgt aagtttttat
     6481 aaatattgat cgatcatttg acgtagttgt tcttttgaat gaacaccaac agctttttct
     6541 actacttgac catctttttt caacattaat gttggaatgc tcatgatacc gaattgttga
     6601 ggagtttctg gattttcatc cacatccatt ttagcaattc tgaattcttc ttcatcatat
     6661 tcttgttcca actgatccaa aatcggtgct tgcatacgac atggaccaca ccaagttgcc
     6721 caaaaatcaa tcaatacaag tcctttatct gtttcttcat taaatgtctt atctgtaatt
     6781 acttgtgaca tatcaaagac ctccattttt aaaatctatg attagtatag catttctata
     6841 cttggatagc gaaccatctg cttaccttta gtaagcttta cttaaattta acaattgtcg
     6901 caccatttcc gccttggttt gccggtgcaa actcaaagct tttgacgctc cgatggtttt
     6961 taagataatc agtaattccc ttgcgaagtg cgcctgttcc ttttccgtgg acaatagtca
     7021 cttgaggata accagctaga atggcagaat ccaaatattg atctacctca cttagcgctt
     7081 cttcgtatcg ttttcctcga aggtccaatt gattcggcac gtgactacta gattcagatc
     7141 gtacagcatg gataactcgt tgtgtcggct ctttttgagg agcaacaggc gtcagctcgt
     7201 cttcggacac attcattttt aatataccga tttctacttg ccactgattc ccgtttttac
     7261 gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac ttcgtctccc ggtttcaacg
     7321 ttttttgttc tttcgccttc ttcaatactt tatttttctt caaagtttct tcttgatgta
     7381 gatttgctaa ttgggtcttt gcatcgatca actgatgctc tttcacattt ccttgcccaa
     7441 tctgctgttg cattttacga atatcagcaa tgattttttc tgctttttct tccgcctctg
     7501 acacgacttc gtttgctttt ttctttgctt tttccatttc tttttcacgt tcttcaaaga
     7561 aatagctgta agcttctttc aaatcatcgt gaagttcttg tgcctcagaa acaaaatgac
     7621 gcatttccag atattcggtc tctgccattt tccggcgatt ctctaaatct gtgatcattt
     7681 cgtttaaatc ttggctctca tcattcatca actgcttcgc ttcatcaata acttctgtat
     7741 caagacctaa tcggctagag atttcaaacg cattgcttcg tccaggaacg ccaatcagca
     7801 aacgatacgt tggacttagt gtatccacat cgaactccat gctggcatta atcgtatttg
     7861 cccgattgta tccataaact ttcaattcag gataatgcgt tgttgccatc acataagcag
     7921 actttttacc aagatcatct aagattgcga ttgctagagc agcaccttct tgcggatctg
     7981 ttcctgcacc gagctcgtca aataatacca gacttttttc attgactttt gataaaatat
     8041 ctacagtgtt cgtcatatga gaagaaaagg tcgatagact ttgctcaatc gactgttcgt
     8101 ctcctatatc cgcaaatact tcctcaaaaa taccgatttg actttcttca cctgcaggaa
     8161 tcggtaatcc tgcttgcccc atcagttgta acagacctaa agtcttcaac gtgatcgttt
     8221 tcccacctgt gttaggtcct gtaatgacga tggcttgata gtcttttccg attgtgatgt
     8281 cattagaaac tactttttcc tgatcgatca gtggatgtct tgcttgtttc aagacgacat
     8341 gattgtcttc actgattccc ggcacgatgg cttttacttc tttaccaaat cgtgcttttg
     8401 cattcatcag atccatttta ccaatcacat aagcattgtg gatgatttct tgacgatggg
     8461 gaacaagttc agcagaaagt tcacttaaaa tacgttgaat ctcatttcgt tcagcgattt
     8521 ggtattgacg caaacgatta ttcaaatcca ccacttgttt tggttcaata aataaagtct
     8581 gtcctgaagc actttgatcg tggactacac cgccaaatac accgcggtat tcttgtttga
     8641 ctgggataac gtagcggtca tttcgcatgg tgatgatcgc atcacttaaa tactttgcat
     8701 ttttcccacg gacgatccca tctaactgtt cacgaacagc ttgttcactt cgccgaatgt
     8761 tctgtcttat gctctttaac tcaggagaag cgtcatcagt cacgcgtccg tcttcatcga
     8821 ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac taactgatct gcccacatat
     8881 acaaacgtgc aaattcgatt tcactgtcag aaagatcatc aatgaaacga ttcaattcgc
     8941 ttgtcgtaga caatactctg gaaacttgtg caagctccac tccattcaag tctgctccga
     9001 tctcgatccg tttcatatgg ggacggatat tttcaagttt aggtataggg atcccgcctc
     9061 gcagacgttg cactttcaat ccatcctcag tttcttgaag ccagtttgtg atcgtttgtt
     9121 tctcactcac tggtaccaat tctgccagtt cttcttttcc ttgtgcagtc actacaaatt
     9181 gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc aaggattcgg ttattcattt
     9241 tatcacctta tctttcacta tgaaaaaaga gctgtgccgg acatacgcta cgcagccttc
     9301 ttaaataaac tactctgtac aaaaaacgaa atattttcca gcactatttt ccttaattca
     9361 aaatattcgt taccaaagct catacatctt gttggtaaga aatggcgttc tctcaacgat
     9421 aaaacgagcg aaactatgct cgccgaattg attttggacc acatctaatg gaacaaaggc
     9481 aagtaactgc aaaatcaaaa atatagtaac gtaagccatg attagtgaca ggatgcctcc
     9541 tgcaatccag tctacctgtc tcaagatcgg cacatacgtc aatccatgga caaaaacccc
     9601 cacaaatcgg gtaagcagcc agccaattag caaaataatc agaaaagcag ttcctgcata
     9661 aaaagcttca tctaggtgaa aagcaaatac ctgatcaaaa aatgctaact ttgatgttgg
     9721 ggtgacagct gggtatggaa tgtataattc caaatgactt gctaatggtt tgtaaaaatg
     9781 ctgggcagca ataaatgaca atacatatcc aatcgcgaat atgatttgca aagcaaaccc
     9841 gcgacgtgct ccagaaaaaa acgcaattag caataaaaat aaaatcagca aactcagcat
     9901 gctacgttcc tttctcttct aactttttta aatttttctt attttgataa aaggatctta
     9961 attagaagat ttttgtttaa tttgtccttt tcgtttctcg tttaaaatct gttgtgcttc
    10021 cacatgattt ttaatggtat gatctgcttg accattttct ttcaatactt cccgagcttc
    10081 gttctcgatt gcttcaatgc gtttcacacg attttcaagc tctgtaaatt tgatcatttt
    10141 tttctttaat tctgctgttt cttcttccag ctccaagatt ctttcttgtt tcttcaattg
    10201 atccgaaagc gcattgacag ccatcaagat tgctgcttgt tcattatcca tttgtggtga
    10261 taattgtttc aattctgcta actgttcatt gatcagcttc gttacgatat ccatatgatg
    10321 cttggtttcc cgtccgataa tagtatatgt ttggttggca atcactgctt tgtagcgtgt
    10381 tttttcgtgc gccatacgaa tcatttcctc ctatttgatg aaagttttat ttagtgtacc
    10441 atgaagttgg ttctttcacc gttctccaca aatatttttc acatacttaa ctattatatg
    10501 ataaacttta aaaaaatgct atgtctcgca atctcttaga aaacgaaaag tcgtgagttg
    10561 cggtatagtt ttatgtataa aaacgaggag aattttatga gcaacgtcat tatcaaagca
    10621 acaaacgacc aacttagtca aatgaaagca tactatcgct cttatttgtt aaataaacag
    10681 attccttata ctagctttgc tgcaaaaaaa gacgggacga ccattaccgc ttatacatcc
    10741 ggaaaagtga tgtttcaagg tacgaatgca gcaatagaag ctgataaatg gggggaggca
    10801 caggataaac aagccaagcc agcaaaaaca gcctcttctt tgccaaaaaa tatcagtcaa
    10861 ttatccgttt taggtagtga tgaggtcgga aatggcagct attttggtcc gctaacagta
    10921 tgtgctgctt atgtgaaacg tgatcagctt gcagaattac gtaaacttgg tgttagagat
    10981 tcaaaagagt taaaagatag ccaaatcatc cagcttgctg acatcttaaa aaaaaccatc
    11041 ccctataaac tgcttgtctt atctccagaa aaatacaatg agatccaacc aaaatacaat
    11101 gccgttcgga tgaaagtcgc tttgcacaat caagcgatcc atttgctgct aaaaaaaatc
    11161 gagccagaaa aaccagaagc gattttgatc gatcagttta cgccggaagc aaattttaaa
    11221 aaatatgcgc gtttagaaaa aaatcaatta caagaaaaac tgtacttcgt tactaaagga
    11281 gaacaatatc atttagctgt tgctgctgct tctatcatca gtcgtgccag ctttttggaa
    11341 gagttacaca aagcatcagc tgaagctgga atcacccttc catcaggagc tggaacgaaa
    11401 tctgaccaag tagcggcaaa actattggag aaaggcggca tgcctatgct tgaaaaatat
    11461 gccaaacttc attttgccaa tacagaaaaa gctttgaagc tgctaaaaaa ataatggaac
    11521 tgtgacaaaa gtcatgtcac agtcccttgt tccgaataaa cggtgttctc
//
LOCUS       DBNIBO010000093        11451 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.097, whole genome shotgun sequence.
ACCESSION   DBNIBO010000093 DBNIBO010000000
VERSION     DBNIBO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.097"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..1675)
                     /locus_tag="AC1Y77_001914"
     CDS             complement(<1..1675)
                     /locus_tag="AC1Y77_001914"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIQ1575477.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNK"
     gene            2223..3725
                     /locus_tag="AC1Y77_001915"
     CDS             2223..3725
                     /locus_tag="AC1Y77_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575478.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3738..4400
                     /locus_tag="AC1Y77_001916"
     CDS             3738..4400
                     /locus_tag="AC1Y77_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575479.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4501..4704)
                     /locus_tag="AC1Y77_001917"
     CDS             complement(4501..4704)
                     /locus_tag="AC1Y77_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIQ1575480.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(4815..5999)
                     /gene="iadA"
                     /locus_tag="AC1Y77_001918"
     CDS             complement(4815..5999)
                     /gene="iadA"
                     /locus_tag="AC1Y77_001918"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HIQ1575481.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(6156..7895)
                     /gene="yfcC"
                     /locus_tag="AC1Y77_001919"
     CDS             complement(6156..7895)
                     /gene="yfcC"
                     /locus_tag="AC1Y77_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HIQ1575482.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(8668..9282)
                     /locus_tag="AC1Y77_001920"
     CDS             complement(8668..9282)
                     /locus_tag="AC1Y77_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1575483.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(9888..10265)
                     /locus_tag="AC1Y77_001921"
     CDS             complement(9888..10265)
                     /locus_tag="AC1Y77_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575484.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(10565..11446)
                     /gene="galU"
                     /locus_tag="AC1Y77_001922"
     CDS             complement(10565..11446)
                     /gene="galU"
                     /locus_tag="AC1Y77_001922"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIQ1575485.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         3419 a         2422 c         1937 g         3673 t
ORIGIN      
        1 ttttatttcg acgttgagta gcttccataa tgactggcaa gatcactggg cggcgttttg
       61 tttgttcaaa taagtaacgg cctaattgat ctcggatgtc ttgctttaat ttgctccact
      121 caaaatcatt gctttctaaa tgtttctcaa cgattgtttc aatcatattg ctgctttctt
      181 tcatcagatc acggctagct tttacgtaaa cgaatccccg tgaagtaatt tgaggttttg
      241 aaatgatttt cttttctcgc cgactgatag tgacaaccgc tacgaaaatc ccgtcttctg
      301 acaagatttt tcgatcacgt aaaacaatgt ttccgatatc acctatgcct aatccatcaa
      361 tcattacatt ttctgcactt gtagttcctg caacactcat acgcttgttc ttatattcca
      421 gcacgtctcc gcgtccagta atatagatat ttttgtatgg catccctact gcatgagcca
      481 aatctgcatg agcagctaat tcacgatact ctccttgaac tggcacaaaa tatgttggtt
      541 tcatcaaatt gatcattaat tgcaagtcat taggattggc atggccagaa acgcgcatat
      601 tctcagagat caacttcact gttccaccag ctcgataaat gatatcttcc gttttcgcca
      661 cgatggtttc catcgcgatg cttggcgtag tagtgatata aaccaaatct ccttcttcaa
      721 tcttgacatt acggtgtgta ccgtttgcca ttttttgcag agacttgatg ggttcaccca
      781 ttcggccagt ttctaaaatg atcagttctt ctggatcata gtttttcatg tctttcaacg
      841 taacaagcag atcttcactt ggtaattgaa gtttttctaa acgcatcgct gtgcgaataa
      901 ttcgttcgaa atcttgacca gtcaagacaa cttttcgttg tgctttttct gctgcatcca
      961 atacttgttg cactcgttgc aaattacttg ctacacaagc gacgatgata cgcccttccc
     1021 aatatttgat cgtatcaaat acttcatctg ctatttgggc ttcagaagct acaggctgag
     1081 gattttctgc atttgccgat tcacttagta aggctaagac tccttctttg ccgatttccg
     1141 ccaatcgtgc ataatctgtt tgatacattg ggatagccgt ttggtcaaat ttaaaatctc
     1201 ccgtataaac gatactacct tcttttgttt tcagattgat ccccactgaa tctggtatcg
     1261 tatgagtggt acggaaaaag ctcactactg tttcaccaaa gtctatttct gtatgctcat
     1321 ctaccacatg gaaatcctta aatcctttgg ctgcttcatt acgactgaca ttcaattttg
     1381 ctaactcgat cgtcaactct gtcccgaata ctggtacaga aatttttgac aacaagtaag
     1441 gaagtgcccc gatcgcatct gcatgtccat gtgttaaaaa gacaccagct acacgatcaa
     1501 tgttttcttc taaatacgta aaatcaggga ttacgacgtc gatccccaac aactcatttt
     1561 caggatattt tagcccacaa tctaaaataa aaatatcctc gcccacttct gcgacataca
     1621 tgttttttcc attttcacga acgccgccta atgggataat tttaatggta ctcacaaact
     1681 tcacctcttc ttatgacatc caagtttaaa gatgtcgatt ttcttcttta tctaaatata
     1741 tgagccattc caatgaagca tatgcaaaat cactatttct caacttatgg agaatcagca
     1801 ggaaaattac cagcacatcc gcgttctgac agttggatgg gcattttatt ttggatatac
     1861 ttcattaaca aaatggatct tttgcgaaac gctatagcca gaatacttat ataagtattc
     1921 agctgttccc cattattata caacaaataa actaaatcgc tagtatcagc aattgttaga
     1981 acaactatgc acgtaaaaat ccctctgtag catctaggat ttcttttgct attcctcttt
     2041 catttcttct tcgtcggttt gaaacatact tttttcattt taccacattt tttgcaataa
     2101 ctatagtgac tttttatata tacagaacct catgttgatg gtgtgcaaac agcataagat
     2161 ttaagctagc tttaagatag gttgtctatt atcaaatgca tctactagga gggatgcaaa
     2221 atatgaattt tatgaaacgt gcatggaaaa gtacaaaagt aaaatgggga cgttcattgt
     2281 tattgtttgc cgtgttcaca gccattctta tttttgtgct agctggcctc acgattcgaa
     2341 gcgctgcaga agaagcagcg aatcaagcac aaaaagaagt tggagcaaca gtgactttaa
     2401 gtgccaatcg agaagcttca tttaaaaaac aggaacaaac ttcttcaagc ggttctacca
     2461 ctgaagaacg tccagatcca ggcagttttt ctcttacacc agtttcctta acggacgcgg
     2521 aaaaaatagc tgaattagat aatgtaaaga gttattcctt tgaaagttcc gcctcagcta
     2581 tggcaaaaga tggaataact gccatcacta gttctgattc aagtgaagac agttcagaaa
     2641 ctactgatgc aggcggtgaa cctgctggag gaatgggcgg gaatccgcaa atgactcaag
     2701 cagactttca aatcagtggt gtgtcagcaa ctgcacaaaa tagtaatttt tctgatggaa
     2761 cagcaaaaat cacggatggt gaaggaatag atgacagcga caaaggcaca aacaatgtag
     2821 tcattgattc tactctagca gaaacaaatg acctggcagt cggagatact ttcacaatca
     2881 cgagtactga agatgaagat accacttatg aaatgacgat caaaggaatt tatgaatcca
     2941 gcgaaacaag cggcagtatg ggtatgaaat tcaacttcat gaatccgtcg aatacacttt
     3001 acacttctta cacttttgca aatgaactaa atgggacatc tgacgataac acgatagatt
     3061 ctgctattta cactctatct gatccagata aaatggatga gtttctcaaa gaagctgaga
     3121 agctgatcga cacagataca tttagcttac aatcaaatga ttccatgtat cagtcaatgt
     3181 tggaaccttt aaacaacgtg gcaagttttt ctaaaaatgt tgttttacta gtggctgtag
     3241 ccggtattat catacttact ctgatcatta tgattaccat tcgggagcgt cggcatgagt
     3301 taggcgtctt gctatcttta ggagaatcca gaagtaaaat cattctccaa ttgtttaccg
     3361 aagtggctat ttgtatgatt cttgcattag gagttgcttc gcttagcgga aacgtcgtcg
     3421 cgaatgccgt gggacagcag ctacttgacc aacagacaga aaccacgaca caaaaccagc
     3481 aagctcctgg cggtggacaa atgccaggta acagacaaga acaaggcggt caacgcgatg
     3541 gtcaacaagg aaccacgaat aatccatttg ctgtttctga tcaagtcagc gaattggaaa
     3601 tcaatgtcaa acctgcacaa ttgggactat tagccggagt agcatttgga atcagtctgt
     3661 tttctgtctt tttagcttct atagggatat taagattaaa cccaagaaaa atcctattaa
     3721 actaaaggag gaatttgatg ttacggacag aacaattggg attttggtat caagatccgc
     3781 aagatgcgct ttataaagat gtagaactga atttcgaagc tggaaaaatg tacgctattt
     3841 taggagcaag tggttctgga aaaacgactt ttctttcgct tatttctgga ctagatgcac
     3901 caaaagaagg aaaggtatac tatcaagatc gttcactgaa caaaatcggg ttaagaagtt
     3961 atcgtaaaaa tgatgtctcg attattttcc aagcttacaa cttgcttcct tatttatcgg
     4021 ctctagacaa cgtcatagca gcaatggaaa tcactaagac caagcaagag aattatcgac
     4081 aagttgcttt agatcgactg gctcgtgtag gaattgatga aatattagca aaaaaacagg
     4141 tgaccaaact ttcgggaggt cagcagcagc gagtcgctat cgttcgtgct atctgctgtg
     4201 aacatcaact aatcgtagcg gatgagccta ctggaaatct agatgaaaca acttcacaag
     4261 atatcgtcaa acttttccaa gaaatcgccc atgaacaaaa tcgctgtatt atcatcgtga
     4321 ctcatgagat tgaagtagca aaaagctgtg atgaagtctt tgaactaaaa aataaaaaat
     4381 ttcaaaaaat cgaagtttaa aaaacaggat aatgatcaaa taaaaaatcc gaaccatatc
     4441 tgtctcgcag atatggttcg gattttttat ttgcttgatt caattagtaa taagtggttt
     4501 ttattttttt tcgtcttttc gagaaaactt gtctttgact tcgtcgccta aatttcgagc
     4561 tttttctttg atgtcagcac ctagtgattg tgctttccct tctgtttctt tgctcttgtc
     4621 gtctgttgct ttgccgtatg cttcttttcc ttcaccgact actttatctt tcatatcttt
     4681 agaacgtcct tttaaatctg ccattgtgct tcctcctctt agtttgttta ttgatctacc
     4741 ttcagggtaa catcaacaac agatacagac aaacagaacg ctcgttttct aaaaaagata
     4801 gttagagctg ttgattaaaa acttttttcg aaagtccccc atactttgac ttctttttct
     4861 tggaccatta tttctccttt agcgataact gtatcaatat caagcgtttc tttatccaag
     4921 atacacagat ctgcatcagc accaatttcg atttttcctt ttttatccaa tttcaatata
     4981 cgtgctggat tactggttat agctcgtaag gcgatttcca aagggatact ttctttttgc
     5041 acagcttctt tgatccctac tagcaatgcc ttggcacttc ctactcctaa ccctaggaaa
     5101 tgattgtttt catcaaaata aggcaagctt ccttgtccat ctgaggtcat cgtgaaatta
     5161 tctaaagaaa catcttcctc gatcaatcgt ttcagtgctt tgctgaagcg aacttcacca
     5221 tctgtcttct cccaaaaatc aggatcttcg ctagcagtga agtcaatcgt gccaccacgt
     5281 ttggcaaatg ccacgcaagc ttcaaataat tctggtgtgc gatttgcatg agtaggcaaa
     5341 aattgtgtga tcggaatttc tgtttcttct acgattcgtg tcaatagctc tagctttctt
     5401 tttcctccgc ctaagtggac attgatgatc ccagcttttc cggataacat gcctcctaca
     5461 cgagcatctg ctactgcacg agcgaactct tcaaaacttg gctgagaaga acggtgatcg
     5521 gatactgcga tttctccgat tccgataatt ttatcaattg tcaaaaagtc tttgatgata
     5581 gaatctgtta ctgtttttac cggcaatcgg tacgaacctt cgtatacata agtggagatt
     5641 ccttctgctt ccagaccttt cgctttgctg atcaacgcag tcatatctct accttctccg
     5701 tctgttccca aacaaccgac aactgtcgtt acaccagctg tagtcaattg gctcaatgtg
     5761 acttctggcg ttcgattttg ataaccgcct tctcctcctc cacctaaaat atggaaatga
     5821 cagtcaatga aacccggggt tagtactttt ccctcacctg aaagttcttc tacttccact
     5881 ccttcgtagc cgccttctag ctgttcttct acagccaaaa tcttcccacc ggcaatcagt
     5941 acatccattt ttcctaagta atctggtgca aacacttcaa tttgtcgaat gattttcata
     6001 catttttcct ctttctttaa gtataactga tgataggatc ttaaaaaata atccaaacga
     6061 taggatcttt actcctttga ttttaccgta aaatgacggt tcttggagtc cttatctact
     6121 gtttggatta cttttttatt tacaggtctc cagtactact gcagacctat tgcaatggca
     6181 atcatgatga aaatagttcc taaaacaaac aggaatcctt gcatcttgat ttggaatctt
     6241 gcccattttg cccattcgat ccgtgcaaca cctagcacac caatcaagct ggctgatacg
     6301 ggtgtaaaag cgtccataaa acctgaacca agctgataag caagaactgc tacttgtctg
     6361 gatacaccta ctaaatctgc cagtggagcc atgattggca tagtcaaagc tgcttgtcct
     6421 gaatttgatg taacaaccaa gttgaacaaa ctttggaaga tgtacatcgc ccaagcacca
     6481 atgacagctg gaacaccggt caacgccgtc ccgatcccat gcaaaatcgt attcaaagct
     6541 gaaggaacag atgcatcaga tcctcccaag acaagaagaa tccctttggc catacctact
     6601 acgattgctg taccagctaa atcagctgca ccagattgga aagaagaagc gatgtcattg
     6661 actttcattc catctaattt gaaaagaaca gcaatgattc cagcaactaa tcccatcaca
     6721 aagaattgag aagcgatttc tggaatgtaa tagccttttt gagtcacgcc ccaaacgatc
     6781 cagacaagga caactaacat ctctaataag atcaatttat gcccaagcat gaatttttta
     6841 ttttcatcag atgtcttttt gatatgagaa cggaaataat catctgattt gtaagtcaac
     6901 gaactttctg gttttttacg gattttttct gcatatatca tcatataacc agcggctaat
     6961 gcagtaacaa ctacccacat aatcaatcgg aatgtcgcac cagacaacac ggggattcca
     7021 gcaatccctt gagccaccgc tacgctaaac gggctcatcc aagaggtcgc atttccgact
     7081 tgagatgcga cataagtcac ggttactgct acgatcgagt catatcctaa cgcaatgaca
     7141 aatggaacca tcaccattga gaatggaatt acctcttctg ccatcccgaa tgtagcgcca
     7201 ccgaatgaaa aagcaaagaa taatagcggt aaagctaaac gctccagtcc cttagtttta
     7261 ctgataaacg catagatacc tgcgtcgatc gctccggtac gcataataat accaaaagca
     7321 cctccaacaa caaggatcaa tgcagcgatc cctacagcag atccatactt atctccagat
     7381 actagtcctt caaaaacaaa atttaagaaa ccaaagccgc caaaatcatc tgttccccat
     7441 attttggctg ttttatgcaa ttttttagaa gtatcataga tattttcgcc gtactgatcg
     7501 taaagcacat catctgtcaa ggagatttca tctagttttt cctgcgtaag atttttttct
     7561 ccgtcagcaa tgactttttc caatcctttt tccggaacat ccagtttttc acgttctgct
     7621 ggatgatctt gcagttcttc taattgatca aacacatacg acttatccag ctcgtaagca
     7681 taacggaagg aatcttgacg taaaacagta cgagtacttg tttcgccatt tgcatctttg
     7741 tattcaatat cttgcgtgct gaactttcct gctggaacga caaaagtcag tatccatgca
     7801 aagatgacta caccaaaaat aatgacatac gtatggggtg tcgataattt agtgaagtcg
     7861 cgtttcggtt ttcctgaaga atttgttttc ttcaaattta tttcttcctt tcttttttca
     7921 gtaattttga tgaataaaaa atggataatc aaatgaatta tacagtaaaa atgacatttc
     7981 cacatgaata atttattaga aaatcacttt tttctatttt gttttttttc tcctatgtat
     8041 gattattttt ttctctttcc atttatatta agtattttat ttctctgttt ttccttaaaa
     8101 ttcttttttt atttttagat tagagaaaat ctcgtcctgt ttttttcatt ttacaatcat
     8161 tgaaaaattg aatttcattg caaaaaaaat aataaacact tataaatatc cattatctat
     8221 ccttcaattt ttgtgtattt tatttctatt agaaaaaggt atcctgttgg acaattttgt
     8281 tatatcaggt aaacttcatt gcaataatga taacaaatgt actataatta ctttgtaaag
     8341 agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa tccatatgga
     8401 ttctttcttg tctctttttc cactactttt tatttttctg acactacata ttcttataac
     8461 tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca aaacaagctt
     8521 aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc
     8581 tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac attatttttt
     8641 taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga ctagctgttt
     8701 tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca
     8761 gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc
     8821 caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg atttgatact
     8881 tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt tgatccactg
     8941 agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc
     9001 tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg tttcatagac
     9061 acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt gatcccaaaa
     9121 ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac caccttgatt
     9181 aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta tccttaattt
     9241 ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct cccgcctagc
     9301 tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa aaaacagtag
     9361 ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata
     9421 cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa tatctacata
     9481 ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac tgcattgact
     9541 atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga ttcttagcgc
     9601 caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt tattgtaagt
     9661 aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga tttatatata
     9721 tataaataac ataaaaaaag gaatggctta tcccgataca acaagcattc ctttaaaaaa
     9781 gattctttta tataagtgaa aaaagctttt ttccaccaat taaataaatc tctcatctca
     9841 atagtttatc cattatttta tctttttttg cgtataccta ctaaatatta ccgtcttctt
     9901 ctcgttcttc tactttgttc gagagaatct gtactagaaa aagtagttgt atgtctacct
     9961 tttctgccta tccgagactt tcttttcata tatgggatat aaatgattag atacagtaca
    10021 gcaaaaaata aagcaaatac gcaagcttcc caactaccta acgccaattc aaccatcaag
    10081 gtagcaaccc caacggatgc tgcataccag ataaaaatca gaaacgtttc tttttttctg
    10141 accatgcgaa acatgataaa tagactaata agcaatagtg gcaacacaaa tatccaattg
    10201 cttccttcca tcagccactt gagtatatca agcagcgact gtgcaacagc gactaatttt
    10261 cccatagtga acacacacct ttttttataa ttaaaaaatt cgctttttta ttctactatg
    10321 aatcttctgg cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa
    10381 aaaaattaaa ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct
    10441 ttcgttttcc acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat
    10501 gacgcacaag ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt
    10561 ccagttaact ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag
    10621 tcccaatttc cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat
    10681 cgcctacgtc gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa
    10741 gagtctgaag tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta
    10801 atatctcaaa tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg
    10861 cttttcccat ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat
    10921 ctgttttagg atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg
    10981 caacagcgga agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat
    11041 catctttcat caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt
    11101 ttgcttgaag cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat
    11161 tgacttgagt cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat
    11221 tgttttccaa ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg
    11281 ttacgatcaa gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg
    11341 ttggtttatc cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac
    11401 gtgttcctag cccagcggca ggaatgactg cttttttgac cttcacttgc t
//
LOCUS       DBNIBO010000094        11449 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.098, whole genome shotgun sequence.
ACCESSION   DBNIBO010000094 DBNIBO010000000
VERSION     DBNIBO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.098"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..1031)
                     /locus_tag="AC1Y77_001923"
     CDS             complement(<1..1031)
                     /locus_tag="AC1Y77_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1575486.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="AC1Y77_001924"
     CDS             complement(1113..1802)
                     /locus_tag="AC1Y77_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIQ1575487.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="AC1Y77_001925"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="AC1Y77_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIQ1575488.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="AC1Y77_001926"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="AC1Y77_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIQ1575489.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="AC1Y77_001927"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="AC1Y77_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIQ1575490.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="AC1Y77_001928"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="AC1Y77_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIQ1575491.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="AC1Y77_001929"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="AC1Y77_001929"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIQ1575492.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="AC1Y77_001930"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="AC1Y77_001930"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIQ1575493.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="AC1Y77_001931"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="AC1Y77_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="AC1Y77_001932"
     CDS             complement(7636..8523)
                     /locus_tag="AC1Y77_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1575494.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="AC1Y77_001933"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="AC1Y77_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIQ1575495.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="AC1Y77_001934"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="AC1Y77_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIQ1575496.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="AC1Y77_001935"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="AC1Y77_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIQ1575497.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="AC1Y77_001936"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="AC1Y77_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HIQ1575498.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="AC1Y77_001937"
     CDS             complement(10454..10957)
                     /locus_tag="AC1Y77_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIQ1575499.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3338 a         2374 c         2090 g         3647 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gctagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaa
//
LOCUS       DBNIBO010000095        11255 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.099, whole genome shotgun sequence.
ACCESSION   DBNIBO010000095 DBNIBO010000000
VERSION     DBNIBO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.099"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="AC1Y77_001938"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="AC1Y77_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIQ1575500.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="AC1Y77_001939"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="AC1Y77_001939"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIQ1575501.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="AC1Y77_001940"
     CDS             complement(2169..2906)
                     /locus_tag="AC1Y77_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIQ1575502.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="AC1Y77_001941"
     CDS             complement(2997..3524)
                     /locus_tag="AC1Y77_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575503.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="AC1Y77_001942"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="AC1Y77_001942"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIQ1575504.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="AC1Y77_001943"
     CDS             complement(4401..4886)
                     /locus_tag="AC1Y77_001943"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIQ1575505.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="AC1Y77_001944"
     CDS             complement(5039..5236)
                     /locus_tag="AC1Y77_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575506.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="AC1Y77_001945"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="AC1Y77_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HIQ1575507.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="AC1Y77_001946"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="AC1Y77_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HIQ1575508.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="AC1Y77_001947"
     CDS             9211..9423
                     /locus_tag="AC1Y77_001947"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HIQ1575509.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /gene="rnjA"
                     /locus_tag="AC1Y77_001948"
     CDS             9425..11104
                     /gene="rnjA"
                     /locus_tag="AC1Y77_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HIQ1575510.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3266 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaata
//
LOCUS       DBNIBO010000096        11150 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.100, whole genome shotgun sequence.
ACCESSION   DBNIBO010000096 DBNIBO010000000
VERSION     DBNIBO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.100"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            6..305
                     /locus_tag="AC1Y77_001949"
     CDS             6..305
                     /locus_tag="AC1Y77_001949"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1575511.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="AC1Y77_001950"
     CDS             378..1739
                     /locus_tag="AC1Y77_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HIQ1575512.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="AC1Y77_001951"
     CDS             1761..2021
                     /locus_tag="AC1Y77_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575513.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="AC1Y77_001952"
     CDS             2035..2787
                     /locus_tag="AC1Y77_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIQ1575514.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="AC1Y77_001953"
     CDS             complement(2881..3486)
                     /locus_tag="AC1Y77_001953"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1575515.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="AC1Y77_001954"
     CDS             complement(3542..4516)
                     /locus_tag="AC1Y77_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HIQ1575516.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="AC1Y77_001955"
     CDS             complement(4894..5613)
                     /locus_tag="AC1Y77_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1575517.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="AC1Y77_001956"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="AC1Y77_001956"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HIQ1575518.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="AC1Y77_001957"
     CDS             7164..8561
                     /locus_tag="AC1Y77_001957"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1575519.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="AC1Y77_001958"
     CDS             8642..9082
                     /locus_tag="AC1Y77_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1575520.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="AC1Y77_001959"
     CDS             9229..9555
                     /locus_tag="AC1Y77_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1575521.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="AC1Y77_001960"
     CDS             9556..10305
                     /locus_tag="AC1Y77_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIQ1575522.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="AC1Y77_001961"
     CDS             complement(10331..10741)
                     /locus_tag="AC1Y77_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1575523.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DBNIBO010000097        10760 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.101, whole genome shotgun sequence.
ACCESSION   DBNIBO010000097 DBNIBO010000000
VERSION     DBNIBO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.101"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(32..310)
                     /locus_tag="AC1Y77_001962"
     CDS             complement(32..310)
                     /locus_tag="AC1Y77_001962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575524.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1042)
                     /locus_tag="AC1Y77_001963"
     CDS             complement(425..1042)
                     /locus_tag="AC1Y77_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575525.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="AC1Y77_001964"
     CDS             complement(1437..2261)
                     /locus_tag="AC1Y77_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIQ1575526.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="AC1Y77_001965"
     CDS             2604..3020
                     /locus_tag="AC1Y77_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1575527.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="AC1Y77_001966"
     CDS             3017..3487
                     /locus_tag="AC1Y77_001966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1575528.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="AC1Y77_001967"
     CDS             3501..4274
                     /locus_tag="AC1Y77_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1575529.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="AC1Y77_001968"
     CDS             4288..5100
                     /locus_tag="AC1Y77_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1575530.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="AC1Y77_001969"
     CDS             5154..6119
                     /locus_tag="AC1Y77_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1575531.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="AC1Y77_001970"
     CDS             6256..8979
                     /locus_tag="AC1Y77_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1575532.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="AC1Y77_001971"
     CDS             8992..9624
                     /locus_tag="AC1Y77_001971"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575533.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9832..10056
                     /locus_tag="AC1Y77_001972"
     CDS             9832..10056
                     /locus_tag="AC1Y77_001972"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIQ1575534.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10158..10391
                     /gene="relB"
                     /locus_tag="AC1Y77_001973"
     CDS             10158..10391
                     /gene="relB"
                     /locus_tag="AC1Y77_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIQ1575535.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="AC1Y77_001974"
     CDS             10381..10650
                     /locus_tag="AC1Y77_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIQ1575536.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt
//
LOCUS       DBNIBO010000098        10608 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.102, whole genome shotgun sequence.
ACCESSION   DBNIBO010000098 DBNIBO010000000
VERSION     DBNIBO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.102"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            46..993
                     /locus_tag="AC1Y77_001975"
     CDS             46..993
                     /locus_tag="AC1Y77_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIQ1575537.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            1127..1756
                     /gene="upp"
                     /locus_tag="AC1Y77_001976"
     CDS             1127..1756
                     /gene="upp"
                     /locus_tag="AC1Y77_001976"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIQ1575538.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1895..2137)
                     /locus_tag="AC1Y77_001977"
     CDS             complement(1895..2137)
                     /locus_tag="AC1Y77_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575539.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            2258..2330
                     /locus_tag="AC1Y77_001978"
     tRNA            2258..2330
                     /locus_tag="AC1Y77_001978"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2291..2293,aa:Thr,seq:cgt)
     gene            complement(2449..3087)
                     /locus_tag="AC1Y77_001979"
     CDS             complement(2449..3087)
                     /locus_tag="AC1Y77_001979"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIQ1575540.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(3210..3842)
                     /locus_tag="AC1Y77_001980"
     CDS             complement(3210..3842)
                     /locus_tag="AC1Y77_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIQ1575541.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            4193..4492
                     /locus_tag="AC1Y77_001981"
     CDS             4193..4492
                     /locus_tag="AC1Y77_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HIQ1575542.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            4987..7152
                     /gene="acm"
                     /locus_tag="AC1Y77_001982"
     CDS             4987..7152
                     /gene="acm"
                     /locus_tag="AC1Y77_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HIQ1575543.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(7424..7909)
                     /locus_tag="AC1Y77_001983"
     CDS             complement(7424..7909)
                     /locus_tag="AC1Y77_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIQ1575544.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    7999..8233
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8389..9030
                     /locus_tag="AC1Y77_001984"
     CDS             8389..9030
                     /locus_tag="AC1Y77_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIQ1575545.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            9049..9684
                     /locus_tag="AC1Y77_001985"
     CDS             9049..9684
                     /locus_tag="AC1Y77_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575546.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            9702..10517
                     /locus_tag="AC1Y77_001986"
     CDS             9702..10517
                     /locus_tag="AC1Y77_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIQ1575547.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         3723 a         1679 c         2068 g         3138 t
ORIGIN      
        1 gccgtaattt ttagtcttca cactaaatct cagggaggaa aagtcatgac tgatcgtatc
       61 tcgttagaaa aggaagcagt agcattcagt gatgcaaatg cgcctcatcc gcgtatctat
      121 gaactagcac cggaagaagg aagagtttta ctagaagaag tacaagaatc acctgtgaac
      181 aaatattccg tcgatattga agacttgatg gtggatactg gcgaatgggg aaaactcaat
      241 attcgtctcg tacgaccaga agggaatatg gataaattac cagttatttt ttatattcat
      301 ggagcaggct gggtttttgg cagtgctcaa acccatgata aattgatcag agaacttgcg
      361 gttcgtacga attccgtcgt tgtctttcca gaatatagtc gatcaccaga agcaaaatat
      421 ccaacagcaa ttgaacaaaa ttatgctgtg ttgcaacagc tgaataaatt agccaagtca
      481 aaaaatctgg atctcgagaa tctaatcgtt gcaggagatt cagtaggtgg taacatggct
      541 acagtcatga cgatcttaac aaaagaacga aagggcctcc caatatccaa gcaattgctg
      601 tattatccag tcacaaacgc tgattttgat acagattcct atcatgaatt cggagaaaac
      661 tattttttga caagagaagg aatgatgtgg ttttgggacc aatatacgac gaatgaagaa
      721 gagcgtgctg aaatcacagc ttcgcctttg agagcaaata taaatgaact aaatgatctt
      781 ccttcagcaa tgattttaac gggagaagcg gatgtattaa gagatgaagg tgaggcttat
      841 gcgcgcaaac ttcgtgatgc aggggttcct gttacacaag tacgcttcca aggaatcatc
      901 catgattttg tcatggtaaa tgctatggat cagacaaagg caacgcgtgc agcaatggca
      961 ttatctactt cttggatcaa gaaaaaagaa taagctttgc ttgtccgcag gaaaacttct
     1021 tttcaaaaac ggattttctt aggaaaaatg gcgagtttga ctagtcaatc acttgatttt
     1081 gttttacaat taaaaagaaa cgacagaaca aaggagagct taaaaaatgg gcaaatttca
     1141 agtgatcgat catccattga ttcaacacaa actaactatt attcgggaca aaaactgcgg
     1201 aacaaaggtt tttcgtgaag tagtcgatga aatcgctatg ttgatggcat atgaagtatc
     1261 acgggatatg ccattggaag acatcgtgat cgaaacaccg atcactgaaa caacacaaaa
     1321 aacgttgtct ggtaaaaaag tagcgattat tccaatcttg cgcgcaggta ttggaatggt
     1381 tgacggtatc ttggaattga ttccagctgc taaagtggga catatcggtc tttatcgaga
     1441 tgaagaaaca ttggaaccac atgaatattt cgtcaaactt ccagaagaca ttgatgcacg
     1501 tcagttgttt gtagtagatc caatgttggc aacaggcgga tcagcaatca tggcaatcga
     1561 tgcattgaaa gaacgtggcg cgtcgaacat taaatttgtt tgtttggtag cagcacctga
     1621 gggagtaaaa gctttgcaag aagcgcatcc tgatattgac atctatacag cttctctaga
     1681 tgaaaaattg aatgaacatg gttatatcgt tcctggacta ggggatgcgg gtgaccgttt
     1741 gtttggtaca aaataagtaa atacgaaaaa gggtgtgaca caacttttgt cacatccttt
     1801 tttggtcatt tttaacgttt gatgcagaac agctttttca cagctttcgt ttgcatataa
     1861 aaaaaccatc ccaaaaatgc agagatggtc aaattcaaat cttttttagt tctttgtagt
     1921 taacgattac tacaccctta ctttgcgtca tcaagttcgc attttctgca gtttcatcaa
     1981 tctcaacaac agcggaattt ttcaattgct ttgaaatcgt tccagtttgc gacttgccgt
     2041 gaattgtgaa cattacgtga gtattgatct tgaatttttc accaatttcc gatggtacga
     2101 tagttgtatc aaattttcca aaagttgcca ttttcataaa acctgccttt cacatcaatt
     2161 ataccacgtt ttatgaaaaa tatcatgaaa ttaaactaat tttccatcgg actctttaca
     2221 atcagccaaa tagagtatat aatttctttt ggataaggcc agcttagctc agttggtaga
     2281 gcaacgcact cgtaacgcgt aggtcgtagg ttcgatccct atagctggca ttaaaagaga
     2341 agacagatat atcaaggatt tttaatccat tgatacatct gtctttttgt ttaaaggaaa
     2401 taaagtatgc tttcttagca atagagcatg tattatttct ataaaaattt aggagaataa
     2461 aacgactagc aatatagctg tgaaatcaaa tatcacatga actgcccaac ttgtaatgat
     2521 cgagttgctt ttaatggcag cttggttgaa taacaatctt gccacccctt gtatcaaaag
     2581 gatgtgtgct acagtgtgta aaatatttcc attatcataa gtactgtaat ggataaatcc
     2641 aaagatgatt gctgacaaga aactagcgac aggaatgctg aacttactag aaaaaatagc
     2701 gagaaaatac actgagaaca attcttctcc aagtatcatg ataggcaaaa taataaataa
     2761 taacgggctg aaagcctcat ttattggatt gcctgttaaa ccccatttca aaatttgttg
     2821 caaaaatacc gagactgcgg cactgattac tacattgata aaaaaatatt tggctatatt
     2881 tttgacagga gcgacaggtt tcgagaacat ttttttcgta tcttttttac caaaaacaag
     2941 tgtaaatgct ataaatccag tgatagttaa agaaaaaatg ctaataatgg atggcgcaaa
     3001 tagcaaccct aacaaaccga gtacaacgac aatagtgccc aatatccctt ttttaagttc
     3061 tatttttttt cctaaaatgt tattcatgat aataactcct actattcagt ttttgaagat
     3121 agagtcagta tacatgaaga gttaaaatag gcaatgtttt tttatggata tttgtattgc
     3181 taaaaattta tcttatatat tcattttgat tatactactt ttatttgtaa aaattttcta
     3241 gcaaaaccaa gtgcttgcaa ctgattaagc gtacaatttt ttagatgttc cgagcgtact
     3301 tcaatcattt caaaggagtt gctacttaaa tcgatatttt ttaaatcagt atcaaaaaag
     3361 ctagcatttt ctaaatcatt agtagaaaac attattttag tgagggaaca gtctacaaaa
     3421 tctgcgcttg ataagacaga gtgctcaaag gtactttctt ttatgtttga accagataaa
     3481 tttacatagt ccattttaca attctcgaat aacgtgttcc caatataact ttccatcaat
     3541 ttagtacctg ttaatttaca attgacaaat ttcacgcgat gcaacatgct ttttgtcaat
     3601 atacagcctg aaaaatcaca gttttcgaat atacagtcgg taatctccat tctttgaaaa
     3661 tcattggaag caaaaaatgc atacttgaaa atagatttgt aaataattgg atggcagatt
     3721 atttggtttt cgatgacctc tttttgtgaa aggatattat acagttcaaa atcttcgttt
     3781 tcatagatat gaaaaaagga tcctttattc aagcttgctt cattaacttt aggaaaaatc
     3841 atatttaatt acctccattt tcataaagtc atattaacaa aagctaaacc aacttacatt
     3901 agcagaaaat gagataggtt ttcgcctttt tcgataacga tagtgtcgct atctcttgaa
     3961 acgttcttag aattgccctg agagacacaa attaggcgta attctaataa aaatcttaga
     4021 ataaaaattt ttactgattt aatttagaat gtttttaaaa taaaaatcgt tgccataaca
     4081 gtgtattgaa aggttttaac aatccagtaa tgatttaaaa aaatctagat gtagcgtata
     4141 ctttgtttat agaatgtagt gggaagaaat atatttttgg gaggaaatcg tgatgtctaa
     4201 aagtgaagaa atagaaaaca tggtactaaa attatatgat ctcgttttaa atccagagat
     4261 caaagaaaag gaacgtatac ttttgataga cgcaaagaca ggcttagaaa aaggtcagta
     4321 ttatcctaaa gtcataaata atttggaacg tagtttacgt ccgttagcaa tcaggggaga
     4381 attgtctaaa ccagtatcac cgttttatat ggagatatca acaataggaa aatttgaaaa
     4441 agaattaggt cgaggaatgg cttcggcacc gattactttt gggcatttat aaaaacgagt
     4501 tattcatctc aatgaattaa aaagtatagt caatctcata atgcaggcta ggttcattta
     4561 tttgaacaga gtctgtattt ttttattcta aataaagaaa cagatgtttc tttatattgt
     4621 attttatcct ttaattaaca caaaaacagc tagtataaac taataatgtt aattaaaagt
     4681 gtattttatg aaactatcgt tatttattaa tgttgcattt ttgtaagtta aaaattggaa
     4741 atatagattg aaaaaatgaa agcaataagt aaaataaaat gaatatatgc gaaaaaataa
     4801 tttacattag gaaaaacctt gtaaaaacag ttttttggca tatttatttt ttataaatat
     4861 ttgtaaaata actatcaatt ttttagttaa aagattggaa atgagtgggg gaggaattga
     4921 agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat aggggagata
     4981 gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat tactaatata
     5041 acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag cagtaatgtc
     5101 acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa agtaaaattc
     5161 tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat caattggcca
     5221 agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat gattgaaaat
     5281 aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt taatgaaaat
     5341 attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc cagaaacgta
     5401 accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga taaaacagct
     5461 actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta ttataaaaca
     5521 ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat taacaataac
     5581 ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg acaaagatta
     5641 gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt ttatagagga
     5701 gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa tttttcagta
     5761 actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag gaaaataatg
     5821 gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa tcattcggaa
     5881 gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa tcatacagtt
     5941 aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt gaagattttc
     6001 aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt aagacgagca
     6061 gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga acagggagaa
     6121 gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga agcacccaat
     6181 tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa cgaaaatgat
     6241 acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat cacagcaact
     6301 aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt attcagagca
     6361 agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga caatggaata
     6421 acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga atatacgttt
     6481 aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta tcaaaaaatt
     6541 gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc aggacaaaaa
     6601 acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac agtaaatcta
     6661 ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga ttggcgctat
     6721 cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac tgtaacggaa
     6781 gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa cagttataca
     6841 ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa aaattttcca
     6901 gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca aatatttgct
     6961 catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc tgtacctaaa
     7021 cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa atctagtttt
     7081 gctgtaatca ttggtttatt gatagtaact atctgtgctg ggatattctt acaaaaatat
     7141 aaaaaagttt agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa
     7201 aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat
     7261 caataaatat ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata
     7321 ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa
     7381 gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa
     7441 ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg
     7501 tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga
     7561 tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg
     7621 ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt
     7681 ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat
     7741 agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg
     7801 aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac
     7861 ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc
     7921 ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa
     7981 ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag
     8041 agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat
     8101 tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga
     8161 tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat
     8221 taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg
     8281 tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct
     8341 cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg
     8401 attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt
     8461 accttgattg gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt
     8521 aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg
     8581 ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc
     8641 gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt
     8701 ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt
     8761 ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct
     8821 gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta
     8881 ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg
     8941 ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa
     9001 gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg
     9061 aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt
     9121 ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta
     9181 cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg
     9241 ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc
     9301 cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg
     9361 aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt
     9421 agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg
     9481 attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct
     9541 ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg
     9601 gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta
     9661 ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc
     9721 tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag
     9781 cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat
     9841 ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag
     9901 ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa
     9961 tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa
    10021 cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc
    10081 tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag
    10141 gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag
    10201 agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc
    10261 tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga
    10321 aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt
    10381 ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag
    10441 atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg
    10501 ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt
    10561 ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaa
//
LOCUS       DBNIBO010000099        10290 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.103, whole genome shotgun sequence.
ACCESSION   DBNIBO010000099 DBNIBO010000000
VERSION     DBNIBO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.103"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(18..770)
                     /locus_tag="AC1Y77_001987"
     CDS             complement(18..770)
                     /locus_tag="AC1Y77_001987"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIQ1575548.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            883..1803
                     /locus_tag="AC1Y77_001988"
     CDS             883..1803
                     /locus_tag="AC1Y77_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIQ1575549.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1876..2811
                     /gene="murB"
                     /locus_tag="AC1Y77_001989"
     CDS             1876..2811
                     /gene="murB"
                     /locus_tag="AC1Y77_001989"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIQ1575550.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3109..4587
                     /locus_tag="AC1Y77_001990"
     CDS             3109..4587
                     /locus_tag="AC1Y77_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575551.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4864..6924
                     /locus_tag="AC1Y77_001991"
     CDS             4864..6924
                     /locus_tag="AC1Y77_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HIQ1575552.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7007..7576
                     /locus_tag="AC1Y77_001992"
     CDS             7007..7576
                     /locus_tag="AC1Y77_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575553.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7751..8557)
                     /locus_tag="AC1Y77_001993"
     CDS             complement(7751..8557)
                     /locus_tag="AC1Y77_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575554.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8695..10209
                     /locus_tag="AC1Y77_001994"
     CDS             8695..10209
                     /locus_tag="AC1Y77_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIQ1575555.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3651 a         1714 c         2032 g         2893 t
ORIGIN      
        1 actttttcac aacctcttta tttcaaatct aattctactg gacaatgatc actgcccata
       61 atgtccgtat ggatctttgc atcagctagt cctggctcta aatctttgct cgtcaaaaag
      121 taatcgatcc gccaaccagc attgtttttc cttgaattga atcggtagtt ccaccaagag
      181 tagacacctt cctgggttgg ataaaaataa cggaatgtat cgataaaccc gttatccaac
      241 agtcgagaaa gagccgctct ttcttctgga gtgaatccag catttttttg attcgttttc
      301 cagtttttca aatcaatctt ttgatgagcg acatttagat ccccacacac gatgactggt
      361 ttttgttttt ttagattttc caaataatta taaaaggctt cttcccattc taatcggtaa
      421 tctaaacgtt ttaattcgct ttgagaattt ggcgtgtaac aggttacaag aaagaaatcg
      481 gaatattcca aagtaatcag acggccttct gtatcatgga tatcgatacc cattccgtaa
      541 gtggcattca atgctggttt tttagcaaaa atcgctgtgc cggaatagcc ttttcgttcc
      601 gcgtaattcc aatattgata ataacctgga agatctaaat caatttgtcc tgcttggagt
      661 ttcgtttcct gcagacagaa aaaatcggcg tctaactcat gaaaaacttc taaaaaattt
      721 ttatttacga tggcacgaag cccgtttacg ttccaagaga taaatttcaa taaaagcaca
      781 ctccttcgaa ttaatttcct tacctttgcg atactattgt acctcatttt agtaaaaaat
      841 ggtgtaagat gaaagtaact aaatgatcca ggaggaaagt agatgaagat tggtgtaatc
      901 ggtgtaggga atatcgcaga aaaagcatat ttaccaactt atgcgaaaaa acaaggaaca
      961 cttgattttt attttgcaac aagaaataaa gaaacaaaag aaagaattca aaaagagtac
     1021 gggttccaac atctatatga aacgatcgat gagcttttag aagaaaaaat cgaagcatgt
     1081 atgatccacg cagcaacaaa agtacattat gaattagcca aaaaatgtct agaaaatggt
     1141 atccatgttt atattgacaa accactgagt gtccatctaa atgaagttcg tgaattgcaa
     1201 gaattagctg ataagaacca aataattttg atgatcggtt tcaaccggcg ctttgctcct
     1261 atggtagaag aattgaaacg tatcccagat aagcggttga tccacctgca aaaaaatcga
     1321 atagcggcaa aagaaacgac agagttcgtc ctttatgatt tgtttttaca tcttattgat
     1381 acagctgtgt atttattaga tgaacccatt ttgagaacaa aataccagat aagagaaaca
     1441 aaggaatttt tggaatatgc cattttacaa ttagaaacac aaaatcaaac ggctatttta
     1501 acgatggatt tagcaagtgg cgctaatata gaaaaatatc aagtaacaag taaaaatggt
     1561 acttatgaag taaacgatct gacccagctt gtcattcaag atgctgatgg aaaaaaagtg
     1621 aaagaatttg gcgactggac aaacacgcta gtcaaacgcg ggtttgagcc aatggtagag
     1681 gccttctttt ctcagatccg tttaggtaaa tcggaaacag aattgcttaa acaaaaagaa
     1741 gttgtaagaa gtcatgaatt atgtgaggaa gtgcttaaag atcactaccg tcatcaactt
     1801 tgaacaagac tggcaagatt gtattaagcg tgatagaata atgaggacgt ttgagaaaag
     1861 aaaggaaaat cctcagtgaa taaagaagaa atagttgaaa gcttagccac gattactata
     1921 ttgtttgatg aaccattgat gaactatacg tttacgaaaa caggtggacc agctgatgtc
     1981 cttgcattcc cgaaaaaaca agaagaagta aagcaaatca tcgattattg ccgaatccat
     2041 gatatacctt ggatggtatt aggaaatgca agcaatctga tcgttcaaga tggcgggatt
     2101 cgcggtgtag tcatcatgct gactgaaatg aaacagattc atgtcaaagg aacgatggtc
     2161 attgcggaag caggtgcctc actgattgat acgacttatg cagctttggc agaatcattg
     2221 actggatttg aatttgcttg cggcatcccg ggaagtgtcg gtggagcggt ttatatgaat
     2281 gcaggtgcgt atggcggaga gatcaaagat gtttttgctg aagtggatct tttgcttgaa
     2341 gatggaacat tgaaaacttt gacaaaagac gaaatggtat tttcttatcg tcacagtaaa
     2401 gtccaagaat tacgtgctat cgtattggaa gctcgattca gtttgcaaac aggggattat
     2461 gaagcaatca aagcaagaat ggatgagttg accgaattgc gtcaatcaaa acagccatta
     2521 gaataccctt catgcggaag tgtctttaag cgcccagtag gacactatac aggtcagctg
     2581 atccaacaag caggattgca aggcctgaaa tggggcggtg cacaagtctc tgaaaaacat
     2641 gctggattca tcgtcaatat cgatcatgcg acagcgacag attatattga attgattgcg
     2701 cacatccaag aagtaatcaa agaaaaattt gatgtgtctt tagaaacaga agtgcggatc
     2761 attggagaaa aacaagcaat atcagaaaca agcagaagtt tagccaatta ataagtaatg
     2821 gaacttaaaa taagtaaaag agcaggacgg tggccgtcct gctctttttt gtggttgttt
     2881 acgcaaatgg cgcaaatctt gtacatttta acacaataaa acaaaaaata tagaatattt
     2941 cctcataaat ttttccattt tttgcaatat tctcaaaaaa aagataaaaa atgagaataa
     3001 ctgatgaaaa aacaagcata atccatcata aaatcagtat aggtgaaaag atgaactttc
     3061 atctgcatat caagtaattg gtatgaggca ttttttgagg tgtaggatat gaagttggat
     3121 taccttatag aagaggaaga tagagaaaaa ctgctgctag ctagatattt agaatttgag
     3181 aaaaagaatt atgtagtgct gcctaagtta tatgaaacat taggtttgtc taaatttaaa
     3241 acaagaaatt atatcataga attaaataaa gaatttactg aattcaaaac aaagccagag
     3301 atccaaattt cagaagaagc agagatccat gtcgaacgtc tgaatttgac ggatatcaaa
     3361 aatttaagaa gaatctactt tcaacgatca aaaattgctc gactgttaac ggagatcgtg
     3421 gattcaaaag taacgatcga aaaatttgca gagaatcatt ttttaagcat atcaagagcc
     3481 tatgtaaaaa gaagagaatt agtctctttt cttgaacagc atcaaataaa gttaaagaaa
     3541 aacatattag ttggggaaga aataaacata cggaacgtat tatttggggt atatttcggg
     3601 gtctttaatg gtttttctat gccatttaga gaaagcgtca tgaatgaagc aaagagtatc
     3661 gtgcaatatt ttgaatatct gttcaattta aaactgtctg caacaaaacg aatcaaattc
     3721 aatttgctaa ccactattag tttgttgagg gcctatggga atcaaccaat cggaaaacgt
     3781 ttcttttcag atgaatactt taagagctca gaaggaatga agatcatcaa ggatttatca
     3841 catatttctt ttcttcatat ccctaaagag cagatcattg atgagatttc ttatatcatg
     3901 ttgtttttga tcacggaaga agaatttgaa ttagatggcg catttggact tgcggaagct
     3961 cattttcatg atatccacat gattagtcaa aaaatgtcag tggaattgat tgatcaatta
     4021 ccattttcga ttgaaatctc tgaagaagaa agactatata tacgaaaaga acttcgtatg
     4081 ggtatgatgc atgtcaatcg aaaaaattgg atattcgact ttgagatgac tagctttacg
     4141 tctaaaaagc agctacagtt tttcttcgag acgtatccaa tattcagtac agcaatccaa
     4201 aaggtcatac aagagaataa acgctatttc ttgggaaaag atgaacgttt catgattcgc
     4261 ttattttatg attatctctt tttattggtc ggtatttgtc cggtatcata ttttgaagca
     4321 ccgattcatg tctgtattga tttttcgcaa ggatctgctt ataccgatta cattatttct
     4381 caagtgaaag gatttaaaaa ttacaatatt gtgattgaga gtcgaatcac ttctcgtacg
     4441 aatatttatt tatctgattg tttacttgaa aaatttaata aagaacagat aatttggaaa
     4501 aatccaccga ctccagatga ctgggaatat tttggaaata ccatcatcag agaaaaaaat
     4561 agacatttaa ctgaaaaaat cagctagtat ttatggatgg cagctttgtc tcacatattt
     4621 attagctgat ttatgttttt ttcaaagaaa aggtaactag atataactag atataactag
     4681 atatattact taacaaaatt aaaaagtaca taagttatta ttccgatttt cttgaaacaa
     4741 taaaaaatca aaaaaaatac tctcctaata gtccatcttc ttttgatttt atttatataa
     4801 tgaaatcatc ttatgaaaat tactacttca tgtttagaca agagtaggga ggactaagaa
     4861 taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg tttagataag
     4921 gaacggtcgt gttctgaaaa acactacgtt aagtttatga tgcgcacgat gctgatcagt
     4981 ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc
     5041 gtacgtacct ctgtttctga gaccactgat tcccctatca ctattactca tactttcact
     5101 gatgcaaatg gaaaacagct acttcctgca ttgacttcct ataataaagt cagttatcct
     5161 ccaacgataa accactactg gttaattcct aacaaaacat tatggacatc ggaaaccgga
     5221 agcagtgtgc cgctttctat cttttccgat atgactgatc ctacaaaagg tgatcagtta
     5281 aaacagatca ctgatatttt gaatcatgct ttgacagata taaaggacgg tggaagcgca
     5341 caagtttctt atgtatatga aaaagattat tcacagtttg aaagaaagaa aatcgatgtt
     5401 ctggcaggga caaaagtttc gatatctgat ctggtagaaa ggctgaagaa cgcagatgga
     5461 acacaaggag atatgtccaa agtaatttca aacaatggga atatcgtgga tacgactaat
     5521 acaggaacat ctactatttc cttgtcctat tttgatccaa ttgctttaaa aacaatgact
     5581 gcatctgctg tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa agatcttcac
     5641 ttaagaacag gagagagtta cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat
     5701 ggaacttcag gaaataaaga agaaatcaaa atgaacggga cagtagatac tacaactccg
     5761 ggagaatatc ccgttgtatt aacttatata gactcgaaaa catcaaatgt ggcagaaaca
     5821 acagctgtag tttatgtaga agcatctgct gcttcatcga ctgtcatcac tcattttata
     5881 gatagtcaaa caaataaaga acttcaagca gaaacacata tgagtagtgt aaatcgtatt
     5941 cctgatttgc gcctacctgc tgaattttct gatcgagtgc caaatataga tttatcgact
     6001 tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa cggattagac
     6061 agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat gaataatagg
     6121 aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa tcgttcatta
     6181 gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga gttgatccaa
     6241 tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt tacaatcaat
     6301 gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac gattatgtat
     6361 agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt acatgtttta
     6421 ccattaaaca aacagataga tcaaaaagaa caaaaaagac aagaagaaag cttgtcagaa
     6481 aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca aacaagaagc
     6541 ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa
     6601 gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc taaaataaaa
     6661 caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga atcagatgac
     6721 cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga gcatctgcag
     6781 ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat tccttcttct
     6841 gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa tcgaaatgaa
     6901 tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa ataagtagct
     6961 gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt tagataaaac
     7021 gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc caatcacgcc
     7081 tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga
     7141 gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt attttcaaca
     7201 aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag agacaaaaaa
     7261 aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc
     7321 ggtttttcat tggttagcag tgaaaccaga ttaccaagga aaagggctcg caaaagcatt
     7381 gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa tctatcttca
     7441 cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt ttacttttat
     7501 tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc aggttttaag
     7561 taaattagat aaataacgaa atggtgaaat aagtcctcag tattgggata aaaaacgaaa
     7621 tagtcattcc tcatatctta cttttttgag ttaatactat cggataacta atggcatgcc
     7681 atacattcaa aaaagaaggg gcagtgacaa aagtattgtc actgcccctt ctcttgtttt
     7741 ttagcatgtt ttatcgtttg atttttttag gaataaaatt cttgatgaac gtaaattttt
     7801 ggtaatcgtt tcgactatag aggtaaccaa atacactgaa aacaactgct ccaataaaat
     7861 taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga
     7921 ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg
     7981 gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga
     8041 tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc
     8101 acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca
     8161 agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag
     8221 ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat
     8281 cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca
     8341 tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta
     8401 atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca
     8461 taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt
     8521 ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc
     8581 cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt
     8641 tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa
     8701 aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca
     8761 gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca
     8821 ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg
     8881 attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg
     8941 aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc
     9001 ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat
     9061 gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga
     9121 tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg
     9181 gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc
     9241 tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc
     9301 cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa
     9361 gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt
     9421 accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct
     9481 gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa
     9541 ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat
     9601 gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag
     9661 gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca
     9721 ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct
     9781 attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca
     9841 gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat
     9901 gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg
     9961 tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg
    10021 aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat
    10081 gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt
    10141 gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa
    10201 aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac
    10261 cgagggtcag ctgattgaac actgtttagt
//
LOCUS       DBNIBO010000100         9981 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.104, whole genome shotgun sequence.
ACCESSION   DBNIBO010000100 DBNIBO010000000
VERSION     DBNIBO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.104"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            322..564
                     /locus_tag="AC1Y77_001995"
     CDS             322..564
                     /locus_tag="AC1Y77_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIQ1575556.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            855..1169
                     /locus_tag="AC1Y77_001996"
                     /pseudo
     CDS             855..1169
                     /locus_tag="AC1Y77_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
     gene            1198..2457
                     /locus_tag="AC1Y77_001997"
     CDS             1198..2457
                     /locus_tag="AC1Y77_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1575557.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            2510..3406
                     /locus_tag="AC1Y77_001998"
     CDS             2510..3406
                     /locus_tag="AC1Y77_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575558.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            3471..4286
                     /locus_tag="AC1Y77_001999"
     CDS             3471..4286
                     /locus_tag="AC1Y77_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575559.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVLTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAVYGFSELNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4296..4971
                     /locus_tag="AC1Y77_002000"
                     /pseudo
     CDS             4296..4971
                     /locus_tag="AC1Y77_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            5177..6841
                     /gene="alsS"
                     /locus_tag="AC1Y77_002001"
     CDS             5177..6841
                     /gene="alsS"
                     /locus_tag="AC1Y77_002001"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HIQ1575560.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            6854..7564
                     /gene="budA"
                     /locus_tag="AC1Y77_002002"
     CDS             6854..7564
                     /gene="budA"
                     /locus_tag="AC1Y77_002002"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HIQ1575561.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7694..8191
                     /locus_tag="AC1Y77_002003"
     CDS             7694..8191
                     /locus_tag="AC1Y77_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIQ1575562.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8376..8636
                     /locus_tag="AC1Y77_002004"
     CDS             8376..8636
                     /locus_tag="AC1Y77_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575563.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(8998..9237)
                     /locus_tag="AC1Y77_002005"
     CDS             complement(8998..9237)
                     /locus_tag="AC1Y77_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575564.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            9523..9837
                     /locus_tag="AC1Y77_002006"
     CDS             9523..9837
                     /locus_tag="AC1Y77_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575565.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         3292 a         1521 c         2042 g         3126 t
ORIGIN      
        1 atccgtcgtt aatcattgtt atcagactga gaaagaaccc ttggtattca agggttcttt
       61 tttcttagca tgtaatttga acaccctgat ccagtttttt tctttaatta ttggtagggg
      121 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
      181 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
      241 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaaa
      301 atgaaagttt gagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
      361 aaaaagaaaa acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
      421 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
      481 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
      541 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
      601 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
      661 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
      721 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
      781 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
      841 ctcctaaaat tagattcaaa gaagatagca aagaggaaaa agatagttct gcagcagcta
      901 tagcggcatg tggattgcta gaactttcca aacaactgcc tgtttcagat cctcgtcatt
      961 tcgattacga agagatagca atcaaaatct tggaagaatt acagaaaaac tatacaacga
     1021 aacgagttaa cggatcaaac ggtctgcttc tccatgccgt ttatgataaa aacagcttaa
     1081 aaggtgtaga tgaatgtgtg atctggggcg attactttta tgtagaagga atcactcgtc
     1141 tagctaaaac atggtactgt tactggtgat gtataagaga agaggaggat tcatataatg
     1201 aagcgatgga aaaaaatgat gattggatca atagttttag gaatgtcact cgtatttggt
     1261 gcttgtggta atcaagaaga cagtagtgaa gaatcagcaa atgaaattac cgtttcgaca
     1321 tggaattatg aaacaacacc tgaattcaaa gcgctctttg aagcattcga aaagaaaacg
     1381 ggaatcaaag tgaaagcagt agatattgct tcagatgatt atgatacgaa actcaccacg
     1441 atgctttctt ctggagacac gacagatgta ttgacgatga aaaatctttt gtcgtattca
     1501 aattatgctt tacgagatca gttagtcgat caaacagaac gaattagaga attagacact
     1561 gatgctgctg aagaaacata tgaaatgtat gatatcgatg gaaaaacata tgcattgcca
     1621 tatcgaacgg acttttgggt actatattac aataagaaaa tgtttgatga tgcaggaatc
     1681 gaatatccaa aaaatctaac ttgggacgaa tatgaagatt tagcaaaaaa acttagtaaa
     1741 aatgacggac aagtttacgg agcatatcaa catatctggc gttcgacaat ccaagctatt
     1801 gcagctgctc aaaacgaggc gaatcttgtt gaaccagatt atcaatatat gactgattat
     1861 tatgatcgag cattgaggat gcaaaaagaa ggcgcgcaga tggattttgg cacggctaaa
     1921 tcaacgaaag taacctatca gtctcaattt gaagaacaaa aagcagcaat gatgtacatg
     1981 ggaacttggt atatggctgg catactcgca aacaaagaag caagtaagac agaagtagaa
     2041 tggggaatta cagctattcc tcagaagaaa aaaggagaat cggtcacgac gtttggctca
     2101 ccaacagcat ttgctgtcaa taaaaactca aagaaacaaa aagcggcgca agaatttatt
     2161 gaatttgcag ccagtgaaga aggagcaaaa gtattagcag gagtaggagt agttccttca
     2221 tatcgtacgg atgagattga tcagctttat tttaatctcg ctggaatgcc aacggatgaa
     2281 atttctaaaa aagctttttc tcctgatgaa atcaaacttg aatttccgat tgatacgtat
     2341 gggcctgcaa tcgataagat tttacaagaa gaatatgact tagttttggt tggagatgaa
     2401 acgccggaaa aaggaacggt aaatatggag aaacgtgtag ctgcggaaaa agaataatca
     2461 tgaaaggaga gaagccaaga gataaaacac tcttggtttc gtcatcgaaa tggagaatag
     2521 tataaataag aaagctacaa atcatttgcg tagaaaacga acgtggatcg cttattcgtt
     2581 tatcgccccg aatttcattg gttttttcgt ttttacatta ataccaatcc tgttttcgct
     2641 tgtactagca tttatgaaat gggattcttt ttcaacgcca gaatttgctg gactagataa
     2701 ctttcgtaaa atggccaacg atgagacatt ttggatttct ttgaaaaata cttttttata
     2761 taccgctgct gtagtgcctg gcacacttgt ttgttcgtta gggctagctt tactactaaa
     2821 tcagaaaatt agagggatga agctttttcg tacgatattt ttctttccgt atgttacttc
     2881 cttagtagca attgctgttg tttggaatat gttgtttcat ccaacagtgg gacctattaa
     2941 tatcttttta agacgattta ttgaacatcc tccaggttgg acttcaagtt caaattgggc
     3001 gctttgggca attgtaattg tcagtatttg gcgagggatg ggttattaca tgattctata
     3061 ccttgcagga ctacaaagta tccaaaaaga actatatgaa gcgtcttcgc tagatggagc
     3121 aaaccgttgg aaacaatttt tgaatgtgac attaccttcg ttgagaccaa caaccttctt
     3181 tgtaacgatc atgcttgtca tcaattgttt caaggtgttt gaccttgtgc aagtgatgac
     3241 ggatggcggc cctggtcgag cgacaaatgt gctggtttac caaatctaca atgaagcatt
     3301 caaaaaattt gattttggtt atgcttcagc tatttcactt gttttgttcg taattgtttt
     3361 gggcgttacg gttgttcaat tttcttggaa taagcgtcgt acttaggagg tgtgatatgg
     3421 aagagagtgt tcgaaatgaa agaaaaaaag caaaaattcg ttcaaaaaac atgcccttta
     3481 ttcaatggtt gggtatgatt ctgctgattg tattagcttt attgacgatc attccttttt
     3541 tatggatggt ttctgcttca ttcaaagcaa ataatgaagt gttaacgatt ccgattcaat
     3601 ggatccctaa aacttggcat ttagagaatt atctggttat ttggtctcgt attcctttag
     3661 tgacattttt taagaataca gcttttttaa gcgttgtgat cacgttgatt cagttgttta
     3721 cttctagttt tgctgtttac gggttttctg aactaaattt taaagggcgc gatattttat
     3781 ttttagcata tatcggcacg attgctgtcc cttggcagtc ttatatgatc ccgcagttca
     3841 ttatgatgag aaaagtgggt ttaacagata cgttgtggtc gttggtgcta ttacaagcgt
     3901 tcaatgcttt tggtgtgttc ttgttgaagc aatattacag tagtatccca gattctttat
     3961 gtgagagtgc gcggatcgat ggcttatcag agtgggaaat ctatcgcaag atcattttac
     4021 ctcttacaaa accggcgatt gccagtttga cgatcatcac attcgttaat acatggaacg
     4081 attatatggg gccattcatt tatttgtctt ctacggaaaa taaaacaatc cagttagggt
     4141 tgaaaatgtt tattgggcta tttgatgcag agtacgcatt aatcatggct gcctcagttg
     4201 tttctgttct tcctgttgct tttatatttt tagcgatgca aaaatatttt gtagaaggga
     4261 tcgcttcatc aggaatgaag gaataggagg tgattatgaa agagcagcgt tttgacaaaa
     4321 atgtttatat ggaattcttt tattggttgt atcaatcatt ggcaagtagc ctttgttttt
     4381 ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt gtcgatagat ggaaggaatg
     4441 tcttttcctt tattttttct ttacttttta ttggtccagg ttgcatcgtg atgataggta
     4501 tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt acgtttgttc ataaagacgt
     4561 ttcgccaatt ttggctaaag ggattttttt attggctatt tgcttggata gttagtgtga
     4621 taatgatttt tgattgtttc ttttttatac gtttttctta tggaaaatgg ttgattccgt
     4681 tgtttgtttt acttgcttgt tttacttgct tgtttatcag tttcctttag catcaactgc
     4741 tggtattttc aggtgagaaa tccagcgtcg aaaccaaatc aagttctacg gatagcgttc
     4801 tactatacgt tgaaaaaatg gtatgtgagt ttactagatt ttttactatt gacaagtttg
     4861 tttctcttct ttttcgtaaa gccccaatgg tgtattctac taggtccttc tatcgtattt
     4921 ggtttgattt acttcaacaa tcgtaaattg atgagaacta tggatctgta gactttataa
     4981 attgaaactt tcaattaaga atgacagttt tttatgtaaa aactgtcatt ctttttcatt
     5041 ttttgaaaca tatggctaaa caaatgaaat aaaacatgct atacttttga atgaaacagg
     5101 tttagaaact gtatcactta tttgtgatta tttctaacta ataattaaaa ataaagcata
     5161 aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt tcgaaatctg tagtagaaag
     5221 tttgattaat catcatgttg attatgtatt cggtatacca ggtgcaaaaa tcgatggtgt
     5281 atttaatgaa ttagaggatc aaggtcctga attaatcgtg actcgtcacg aacaaaatgc
     5341 tgcatttatg gcacaagcaa tcggacgtat tacaggagag ccaggagtag ttatcgcaac
     5401 aagtggtcct ggtgctagta acttagctac aggtttggta acagcgacag cagaaggtga
     5461 cccagtttta gcaattgccg gacaagtaaa acgaagcgat ctattgaagt tgacccatca
     5521 aagcatggat aatgcagcac tgttccagcc aatcacaaaa tacagtgcag aaatccaaga
     5581 tccagaaaca atttcagaag tgattgccaa tgcttatcgt atggctaaga gttcaaaaaa
     5641 aggcgcgagc tttatcagta tcccgcaaga tgtagtggat gcacctgtcc aaggtaatgt
     5701 catcaagcca ttgagcgatc ctaaattagg ttctgcttct gcagatgata tccgttacct
     5761 agctgaacgc attagagaag caaaactacc ggtactatta gtaggtatgc gaggatcaag
     5821 tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag acagcattgc ctgttgtaga
     5881 gacattccaa gcagctggag tgatctcacg tgaattagaa gcgcatttct ttggtcgtgt
     5941 gggtttattt agaaaccaac caggagatat gcttttgaaa cgaagtgatc tagttattgc
     6001 aattggctat gatccaatcg aatatgaagc aagaaactgg aatgcggaaa aagatgcgcg
     6061 gatcattgtg atcgatgaag cgccagctga aatcgatcct tttatgcagc ctgaaagaga
     6121 attgatcggt gatatctcag ccactcttga tttattgaca ggttcattag agcctcaaca
     6181 agtgtcagaa gatgcaaaag aatatttagc atctcttcaa gcaaaattaa cggagcggga
     6241 cattgttcaa tcaaaaggag aagcaggtat cttgcatcct ttagaagtta ttaacacgct
     6301 tcagtcaaaa gtaacagatg atatgactgt taccgtagat gtaggtagtc attatatttg
     6361 gatggcacgc cactttagaa gttatgaacc tcgtcatttg ttattcagta atggtatgca
     6421 aacgcttggt gttgcattgc catgggcgat ttctgctgca ttggtgcgtc caaatacaca
     6481 gatcgtatca gtttccggcg atgggggatt tttattctct gcccaggatt tagaaacggc
     6541 ggtacgtaaa aaattgaata tcgttcattt gatttggaat gatggacact acaatatggt
     6601 agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg gtccagttga
     6661 ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc cgagctacaa gtgtagagga
     6721 attagaaaaa gctttagaag aaggatttgc gacagaagga cccgtaatta ttgatattcc
     6781 gattgattac cgtgataatg agaaattggg agaaacaatc ttaccagatc aattctatta
     6841 aggaagtgtc gacatgactg aaaaaatctt ataccagcac gggacattag gagctttgat
     6901 ggccggcttg atggatggaa cagaaacgat tgctcatata ttagaaaaag gaactctggg
     6961 attgggaaca ttgcatgggc ttgatggtga agtcatcttt ttagatggcg tagcttatca
     7021 aggaagatca gatggctcag ttgcccaatt agatggaagt gaacttaccc cctatgcagc
     7081 aattacagat tttacgccag atactagttt ttctgtctca aaaactgcag atggagaaca
     7141 attgaaaaaa gacatattag taagagaagc cggggaaaac ctatttttgg cagtgaagat
     7201 aactggatta ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa aaccttacag
     7261 aagactagca aaaatttcag agtctcaacc agaattccaa caaagcaatg tccaaggaac
     7321 attagtcggg ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg gatttcacct
     7381 gcattttatc gatgatacac atacatttgg cggacatgta atggattttg aagtggctga
     7441 aggaaaagtt gagattagcc gcatctcgtc ccttgtacag cattttccag ttgatgatcc
     7501 gacttttgtt cacgaaacga ttgattatgc tgatttagca gcagaaatcc aacaagcaga
     7561 atagaaaaag acagcgagat gcgttcaagg tgattaacct gacgtatctc gtttttttga
     7621 ggagaaaacc tagcgtttac aaaaaaactg agctataatc ttctccatag atcaaaaagg
     7681 agcggttttt atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa ttttattatt
     7741 acagcaacaa ggattattag aagcagaaga taactggtta gcaaatttag caaattcatc
     7801 tgcattgttg aatgagactt tgcctgagac tgtctttgca ggatattatt tattcgatgg
     7861 taatgaattg attttaggac cgttccaagg gcgagtttct tgcacacgta tcaagatggg
     7921 aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg atgatgtcaa
     7981 aacccatgca aattacattt cctgcgattc agccgcacaa tcagaaatcg tagtgccaat
     8041 ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat tgcggagtaa cgaaagggta
     8101 tgatgaaatc gatcaagcat ttctagaaaa atatgtagat cttttattag caaattttaa
     8161 tataaatgct ttttcattta ttaataacta aaatcgtcta ttgaaacaaa aaagaagaga
     8221 aatcaagaat aaaaagagcc ggaaagacag tgggcatttc tgtttttcgg tttttttatt
     8281 tttttataaa atcacaagtg ttattgattt tcataatcat attttaatgt tattatcatt
     8341 ttaaataatg aaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag
     8401 gaaaaaaata tatttgccaa cctattggct tgaaacaaaa agtgattgga gaagtaatca
     8461 gtaaattaga aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg
     8521 aaatattaga aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa
     8581 caacagaata tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat
     8641 ttaaataaag tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg
     8701 gcagtgattg ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg
     8761 taacgaggcc tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc
     8821 ggatagacaa actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa
     8881 aactgatatc aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca
     8941 tttagttagg agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta
     9001 attcagagtt tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc
     9061 agaggtagaa acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat
     9121 tgttgaatcg atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt
     9181 cccacaagca gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat
     9241 gtacacactc cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa
     9301 tgcaaaacta tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg
     9361 gtgatatccc agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct
     9421 aaacgctttt tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg
     9481 taattttttt gggtatttaa atagttgcta ggaggatagc ttttggattt ttacagtaat
     9541 tttatcttaa taatagcaat attattactt ctaaatattt ggttttttga taagagtaga
     9601 aatgcaggga taggttttag aaccaaacga agtacgtctt ctgaaaaaaa gtgggtatat
     9661 tcacagacta ttttttacgg aggggttatt tcaatcagtt tactttcttc aacactttat
     9721 tcttttaatg ttatagacgt ttccatgtca aattttatat ctattattgg aatccttatt
     9781 tctgcaatta ttacacaact acttcttgtg tttgaagaga agtcaaaaaa taattaaagc
     9841 aattatgcct tttagtacag gaattattac ttcttacaaa tgtatagcga atgtgaagcc
     9901 tgctgtttta attgttctag aaaaactttt gagtgaacaa ttattttatg ttaggatgaa
     9961 ttaatttctc tagagtttat a
//
LOCUS       DBNIBO010000101         9963 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.105, whole genome shotgun sequence.
ACCESSION   DBNIBO010000101 DBNIBO010000000
VERSION     DBNIBO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.105"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            48..626
                     /locus_tag="AC1Y77_002007"
     CDS             48..626
                     /locus_tag="AC1Y77_002007"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIQ1575566.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            630..1151
                     /locus_tag="AC1Y77_002008"
     CDS             630..1151
                     /locus_tag="AC1Y77_002008"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIQ1575567.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(1230..1784)
                     /locus_tag="AC1Y77_002009"
     CDS             complement(1230..1784)
                     /locus_tag="AC1Y77_002009"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIQ1575568.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            2037..2312
                     /gene="rpsP"
                     /locus_tag="AC1Y77_002010"
     CDS             2037..2312
                     /gene="rpsP"
                     /locus_tag="AC1Y77_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIQ1575569.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            2324..2575
                     /locus_tag="AC1Y77_002011"
     CDS             2324..2575
                     /locus_tag="AC1Y77_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIQ1575570.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            2699..3490
                     /locus_tag="AC1Y77_002012"
     CDS             2699..3490
                     /locus_tag="AC1Y77_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575571.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            3660..4181
                     /gene="rimM"
                     /locus_tag="AC1Y77_002013"
     CDS             3660..4181
                     /gene="rimM"
                     /locus_tag="AC1Y77_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIQ1575572.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            4171..4932
                     /gene="trmD"
                     /locus_tag="AC1Y77_002014"
     CDS             4171..4932
                     /gene="trmD"
                     /locus_tag="AC1Y77_002014"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIQ1575573.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            5068..5415
                     /gene="rplS"
                     /locus_tag="AC1Y77_002015"
     CDS             5068..5415
                     /gene="rplS"
                     /locus_tag="AC1Y77_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIQ1575574.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(5547..6683)
                     /locus_tag="AC1Y77_002016"
     CDS             complement(5547..6683)
                     /locus_tag="AC1Y77_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1575575.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(6800..7492)
                     /locus_tag="AC1Y77_002017"
     CDS             complement(6800..7492)
                     /locus_tag="AC1Y77_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575576.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(7630..8283)
                     /locus_tag="AC1Y77_002018"
     CDS             complement(7630..8283)
                     /locus_tag="AC1Y77_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575577.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(8333..8761)
                     /locus_tag="AC1Y77_002019"
     CDS             complement(8333..8761)
                     /locus_tag="AC1Y77_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIQ1575578.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(8766..9140)
                     /locus_tag="AC1Y77_002020"
     CDS             complement(8766..9140)
                     /locus_tag="AC1Y77_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575579.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            9425..9610
                     /locus_tag="AC1Y77_002021"
     CDS             9425..9610
                     /locus_tag="AC1Y77_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575580.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            9623..9871
                     /locus_tag="AC1Y77_002022"
     CDS             9623..9871
                     /locus_tag="AC1Y77_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575581.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
BASE COUNT         3206 a         1738 c         1843 g         3176 t
ORIGIN      
        1 ttttattctt tactaataca attactctaa acgaagaggt ggcacagatg agatgtccgt
       61 ggagtgaaca aacagaaagt atgcggcaat atcatgatac tgtctggggt gttcctgaat
      121 atgatgacca aaagctgttc cgaaaattga tgcttgatat taatcaagcc ggattgagct
      181 ggcagacgat tttaaataaa agtgaagcat tcgatgaagc atatgatcga ttcgatatta
      241 aaaaagtcgc aaattatgga gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta
      301 ttcgtaaccg aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc
      361 aagaagaaat cggcagtttt tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg
      421 attcttctta tcattcacaa gaagaagtcc caacaacaag tcctctttca gatgaattag
      481 caaaagatct aaagaaaaga ggatttaaat ttatcggaag cacaacaatc tatgcttttt
      541 tagaagctgt cgggatcatc aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg
      601 tacgtatcgg cagaaagggc gaataaagca tgcgaaaaat catattttat ggtgctataa
      661 gtttagatgg ctatttggca acgaaaacgg atgatcttca atggcttttt gacactccaa
      721 caggtaagca aaccacttat gaagcttttt atgaaacaat cgatacgacc atcatgggca
      781 gaaaaactta tcaggaagca aaaaaatatt tggagaccga agcaatttat ccagagaaaa
      841 agaatttcgt tttttccacc cacgcacaat tacaattgcc agatgctgag gtcgtaaacg
      901 aagatcctgt tgcttttcta aaaaatctac aaatgacaga aggaaaagat atctgggttg
      961 tgggtggcgg tcgattgatc aaaccgctat tggaacatca aatgattgat gaatggtata
     1021 tccagattac ccctgtatta ttaggggacg ggattccttt attccaaaaa ggaacatatg
     1081 aagcgcgttt tgaaatgcta gacacgacac gattcggtga atttatcgaa ttgcattatg
     1141 tacgaaagta gaagtaaata aaataaaaaa ggcaactata tgcagaaatg tcctgtacat
     1201 agttgccttt tttattaccg ttatgctttt tactctgtta atttgttaga aaaatctaga
     1261 tacgcatcta aaatcgtttg gaagaaactt aatgttcctt caacgatatt accattttca
     1321 tcaattagat tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt tggcacattc
     1381 aaaaagacaa gtgattgtcg tagatggtgg tttgcgccaa agccgccgat cccacctggt
     1441 gatgcagtaa cgactaaacc tggtttatga tcccaaacac tgtgtccata aggtcttgag
     1501 cctacatcta aagcattttt caatactgct ggcacagaac ggttgtattc aggagtaacg
     1561 aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac gagtccattc agcaggagcc
     1621 tgttctggag tttcgagatc ctcattgtaa aatggcagat catcgatttt tacaaataca
     1681 ctttcgtacc cttcaggaag caatttcgca aaagcttctg ctactttttt gttgtaagaa
     1741 tcttttctta aactaccaat aaaaaatcca attttttttg tcatacgatt tttcctcctt
     1801 caaactatct ataacaatct tacaaaaaat aagtaactgg agcaaataaa aacacttact
     1861 gacaaaagct acatagaaga tagattactt atttttaaca aaaaagaagt ctaaaatctt
     1921 ctatttccta aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct ttacagggta
     1981 aaaaaaaact gttacactac atcttgtgaa taaaacaatg gaggtggaaa taaataatgg
     2041 cagttaaaat ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg
     2101 tagctgattc acgttctcct cgtgatggac gtttcatcga aactgtaggt acttacaatc
     2161 ctttgaaaga ccctgcagaa gtagttttaa aagaagactt agttcttgac tggttatcaa
     2221 aaggtgcaca accttctgac actgtacgta acatcctttc aaaagaaggc gttatgcaaa
     2281 aacaccacga agctaaattc tcaaagaaat aaggtgaccg gatatgaaag atttaagcga
     2341 cttagtctta acaatcgttc gtccgctagt cacttatcct gatcaagtca agttagacgt
     2401 cgtagagtcg aaagactttt acgagtacaa tctaacagtg gctcctgaag atattggccg
     2461 tatcattgga aaacaagggc gtgttgctaa agcaatccgc acaatcgttt acggtgttcg
     2521 gatcaatgca ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa
     2581 cgtgctgtac tcgaattttc gagtgcagct tttttgtttt tttattaatt gaagaaatga
     2641 ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat
     2701 gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac
     2761 tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga
     2821 aaaaagtagt accaaaacgg ctaccaccac tgaacaaaca atggctacct caaataatga
     2881 gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga
     2941 tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc
     3001 tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga
     3061 aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca
     3121 agttttgcag ccctttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat
     3181 cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg
     3241 gcgtattcga atccgtggga ataatataga aggccaagat cccttactat tagctaaaga
     3301 aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac
     3361 tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa
     3421 tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa
     3481 gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt
     3541 aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact
     3601 agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt
     3661 tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc
     3721 gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgttaacac
     3781 tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa
     3841 attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg
     3901 atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt
     3961 accatgagat catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag
     4021 aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata
     4081 tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc
     4141 gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca
     4201 agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta
     4261 ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat
     4321 gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac
     4381 atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca
     4441 gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta
     4501 ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa
     4561 gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat
     4621 gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct
     4681 tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg
     4741 aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa
     4801 gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca
     4861 cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat
     4921 gaagccaagt gactttttct aaaatattct ttacagtagg ttgataatat gttagtattt
     4981 tatggtgagt gcaaaagcac ttaactatta cgatattccg ctatgagaaa agctcataag
     5041 aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa
     5101 caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa
     5161 gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc
     5221 ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa
     5281 cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa
     5341 gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa
     5401 gaaatccgtc gttaatcatt gttatcagac tgagaaagaa cccttggtat tcaagggttc
     5461 tttttttatt atctgttttt ttatattaac gatttctatt tggtacattt ttgattaata
     5521 aaaaggggca aaaaaggggc aacaagttac agtttttcta tatcttcttt caatttttct
     5581 tttactcctt ttgtcacatg caaataaatc ttttcagtga tatcactatt ttcgtgccca
     5641 acgcgatcct gaatagcata cagaggagtt cctagttccg ctaattttga aatatgggta
     5701 tgtctaaaaa tatgtgaact aagtttttta tcaatcttca tgtcagcttt atgatttctc
     5761 aaataggtgt taattgctgt tagttggaaa ggagttcctt tcgttgtttg aaatagaaat
     5821 tgacccttcg gatttaattc tagaagctca ttgtaaatag ctatcgcttt ttttggtaaa
     5881 tcaatttctc gcattccagc agcagttttt gtagaatcag attttttcat atcagctatt
     5941 gaacgttctc tatacatcat cgtcccattt ataactactg atgcattatt attggtaatg
     6001 tgtacatcgt ctttacttaa tgcgattgct tctccaggtc tcattccagt caaatatagc
     6061 cactggaaaa ggagagaata tcttttattg tgtgaagttg tgaaatctac tagtctttta
     6121 tattcatcat cttctaaaaa cttatcttta atttttatcg tttttgactc tcgtttataa
     6181 tcaataacta cttcgttgat aggatttttt tctacatagc cttttttcat agcgtatgaa
     6241 aaaagaagat tcaatttgga tttaattaca ctgacgtact tatttgacaa gtcatcttta
     6301 tatatcatat cttcaaaaat attatttaaa tctactgtat taataccaga aacgatgtaa
     6361 gtctcaggca tcttcttctt tatagtgttt aagatattat tcgtaggata atatgtagac
     6421 tccttaactt gccttttgta aataactaac cattcttcga caagctcatg aaatgttaga
     6481 ttaggcttct ggagtgtttt ttcatttagt tttatatcaa tttttttatt taactccagt
     6541 aatgccactt tctgagtttc tcgagactta tttttataag taatgctcac tttttttctt
     6601 tttctagttt ttggatcaat atatcgttca ttatatttat atactttttt tccgttttta
     6661 tcagttttgg tttcaatcca cattgttttc atctcctatt tgttgctata ataggaatgg
     6721 ataagtaagc cgattatagc aggtttattt ttcatcacgt ccacaaactt tggcgaggga
     6781 gggggcgtgt ttttgtattt taacgattta tattgaatgt tgattcagca ctttcagcaa
     6841 ctgtactata atcagacgga ttatataaca ctccggtttt tatcgttact tttgtaatat
     6901 cgttaacttt agatggatct aagtaagcta aactataaaa ttctttactt tcagtttttt
     6961 tgaaaaacga gtttgttgtc attgtatgca taagatcgtc tgaactgaaa tcaatttgtt
     7021 ggccgttatc aagtatggcg tgtgcaagcc cattataacc ataatctact tcagaattgt
     7081 ttttaacttt gaatgcaatt tgtaaaccat aaaaatttcc ttcactatct ttgtgattat
     7141 ttgcatcgta gtaaccagca gcgctagggt cagcataatt ggaatacttg agtagtttga
     7201 tattagatat ataaatgccg atatcaccaa gaggaatatc tgattgtggg cttgccgttc
     7261 caattaattc aattttgcca cttgtatctc ttttttcttt ggtccattga ccaactttga
     7321 ggagggggcc atcaacctcc tgtgaatttt tagaagactg tgttgtactt tctgataacg
     7381 taactgaaga gtctttttcg tttgttttgc cagtattatc tacaacatta gattggctac
     7441 atccggtaag aaaaaaagca ccaaatacta accctaaaat cacttttttc attctttatt
     7501 ctccgtttct atgatattat ttttatgtag gatcttagaa acgagatctt agtccgtgtt
     7561 gcagcacggg cttttttctt tataactttt ttagagatta taggcaaaat agtaggacac
     7621 aaaaatatat tattgtattc cgtatttaga aaatcctaat tgaacttcac cagaagtttt
     7681 ttgctgtgtt gtacgcaatg cttcttcttc agacattcca ttctgtactt tccatgcaac
     7741 aggcgacatc ccgtatttgt taacaaaatc agtaagtgat aaagtgtcag cgtcttgctg
     7801 accgcttgtt tgttggtctt ctggattttg ttgagatgct gcttgttgtt ctttctgatc
     7861 ttgactgata atattgccag catcatctgt agtcaatcca ttttcataaa gggccacgcc
     7921 gaaagcttcc cactctttgt tggaccaatt tgcacgatca gctggagttg actgtaaagt
     7981 gcgttgtttc atctgttcat atgtttcttc ttgaggtgcg gtttggattg tatcctgact
     8041 ggagcttata actgttgggc taggttccgc cgtagcttgg ttagagcttg taactgttga
     8101 actagaatct gtcttagatg tagatttgct agtagaggaa ctggtttcag ttgtttcttt
     8161 tgttttactt acttttgttt cttggttaga agtggcatct gttgattcag cttttttatt
     8221 atttgaacaa gctgaaagta gcagagcagt acttaacaac aacataacgc taactttttt
     8281 cattttataa ttcctctttc tcgttgtaat atgtgtgcta acacgggctt ttttatataa
     8341 gaaaacgata agcgctttct ggaagtccgt aaagattctt taattcatcg atttttttgg
     8401 gatattgatc attatcttct ttataaagag aaacaatgag attagcagca aagcaattag
     8461 cttcgctttc agatttgctt ctagatgttc ttgttgatac atagtaactg gataagccgc
     8521 gatgaaaaat agcgtgacct aattcgtgag cgcaaatgta gaatctttcc tcagagtctc
     8581 gcagttcatc atttaagaag attattgcac gacctctaat ttcttgaaac tgtcctttgg
     8641 gattttcgat aaaaggaacg tattgaattt taatgcccat cttttcacaa atataaaaag
     8701 gattagcaga ccggtatttc cgcttcaact cctcgactaa attaatcgta tccatctcca
     8761 taagctcaca tctttttgcc tttttctttg tcttctttca caatatccca gaaggttcca
     8821 ataaggatat cttttacgcg ttggacctgt tcaggcgtca gtgtttctcc gccataagac
     8881 atattgacat ttgaatctag taatttatca agttcaacta cttcatcttt tgttgcccag
     8941 tcaggaactt ggtttctacc gagtaaataa tcagtagtta cattaaaata atctgctaca
     9001 agttgtagtc ttttagtact aggagttcta tttttccatt gataaatcgt gtttttaggt
     9061 atatttaatt cttcttccaa ttgagttata cttatgcctc ttttatgagc taattccttt
     9121 attctatcta gtaaattcat tttcgcacct cacaagctac gaaaacacta ataaaaaaat
     9181 tagttaaagg tgttgacaac taataaaatt gttagtatac tgttttcgta agctaaaata
     9241 ttagctaaaa agacttgata actaataaac acttcacggt cggcaaactc tgaaatgtaa
     9301 attactaggc atttctgtgt cttatttagc tatatctata tactaataaa aatattagtt
     9361 attgtcaacg aatattagct aattttttag cttacagact attttttaga aaggagctat
     9421 ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa gaagaatgac
     9481 tttcaaagaa ctagctgcgc ttgttggtat ttcaggagct tatttatcag atattctaaa
     9541 cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa aaatattgga
     9601 catccgatag ggggggtagg cgatgacaaa actaaaaaaa caagattttg taaaaaaata
     9661 caattattct ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata cagcaatttt
     9721 ctcagcggcg tatgaacggg tcacaggaca agaagtttgg atcaatacag aattatacga
     9781 taaatttttg tctttcaaat cctataacag gttacgcaca agaaagataa cacctaaaga
     9841 atttatcgag aagcatttag ttgatttata aaaaacagaa cattttgaga ggtgaaggtt
     9901 aatgggcaaa ttcaacagag cactagtatt cagcacacca ttcatcatct acgctttagg
     9961 cct
//
LOCUS       DBNIBO010000102         9958 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.106, whole genome shotgun sequence.
ACCESSION   DBNIBO010000102 DBNIBO010000000
VERSION     DBNIBO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.106"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..658)
                     /locus_tag="AC1Y77_002023"
     CDS             complement(<1..658)
                     /locus_tag="AC1Y77_002023"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575582.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     K"
     gene            complement(663..1430)
                     /locus_tag="AC1Y77_002024"
     CDS             complement(663..1430)
                     /locus_tag="AC1Y77_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1575583.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1919..2344
                     /gene="lacA"
                     /locus_tag="AC1Y77_002025"
     CDS             1919..2344
                     /gene="lacA"
                     /locus_tag="AC1Y77_002025"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HIQ1575584.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2361..2876
                     /gene="lacB"
                     /locus_tag="AC1Y77_002026"
     CDS             2361..2876
                     /gene="lacB"
                     /locus_tag="AC1Y77_002026"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HIQ1575585.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2887..3819
                     /gene="lacC"
                     /locus_tag="AC1Y77_002027"
     CDS             2887..3819
                     /gene="lacC"
                     /locus_tag="AC1Y77_002027"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HIQ1575586.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3822..4802
                     /gene="lacD"
                     /locus_tag="AC1Y77_002028"
     CDS             3822..4802
                     /gene="lacD"
                     /locus_tag="AC1Y77_002028"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIQ1575587.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4830..5147
                     /locus_tag="AC1Y77_002029"
     CDS             4830..5147
                     /locus_tag="AC1Y77_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIQ1575588.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5147..6853
                     /locus_tag="AC1Y77_002030"
     CDS             5147..6853
                     /locus_tag="AC1Y77_002030"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HIQ1575589.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6995..8401
                     /gene="lacG"
                     /locus_tag="AC1Y77_002031"
     CDS             6995..8401
                     /gene="lacG"
                     /locus_tag="AC1Y77_002031"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HIQ1575590.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8424..8697
                     /locus_tag="AC1Y77_002032"
                     /pseudo
     CDS             8424..8697
                     /locus_tag="AC1Y77_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            8718..9617
                     /locus_tag="AC1Y77_002033"
     CDS             8718..9617
                     /locus_tag="AC1Y77_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIQ1575591.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3139 a         1678 c         1996 g         3145 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagcagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttt cgtgtattta ggaaaagcaa catcagacca acatctgaaa cgtttgtaaa
      541 tgttatctat ttgttggata tcaatcgttg gaaaacattt ctcaacgcta cgtttaaagg
      601 ggagttgatg gtcataaaga gtgtcatcaa ggtcaaaaac aatattttta taagtcataa
      661 ttctagtccg cttttaaaat agtagtgtac tggctaaatt cctctaagtg ttgagggcta
      721 atctggttat ctgtaatgat ggtatcgagt tgatctagat tatagaagtt aaagaaatcg
      781 tatcgatcga atttagtact gtctactaat aagaattttt ctacagcatt gtttagggca
      841 agttgttgaa tcacaccttc cttgtcacta tatgtagcaa tagaattatg ggtaacagca
      901 tttgcactaa caaaagcttt ggcaaatcta attgctttta aatttgtcga agccattgaa
      961 cctacaaaag ctccagttat ttctctatat tcaccgccaa gaagcaataa atcaatggtt
     1021 tcgctatcat ttagaatcaa gaacacaggg agactatttg taatgacacg aattttataa
     1081 cctttacgac ctttcaattc taatgccagt tgttcaagtg tagttccggg tccaataaaa
     1141 atagtatcgc catctttgat taaagagcag gcttttttag caatgtcaat tttttctttt
     1201 gtatttagac ttttcttctc gatatgtgtc ttttcaagtg gctttttaga ggaaagtaat
     1261 tgtgcaccac catgagtacg tgttaaaagt ccatcagctt ctagagcatc aaggtctcta
     1321 cgggctgtca tatcggaaat atctagttca tctattattt cttttatggt tatggtccca
     1381 tcaatcttta acatatctaa aattttttct aatcgtcgtt ttttgttcat atgaagaccc
     1441 ttctttcata aagtaatttt tttccaaaga taattctctt ttaattgtat cataaaagac
     1501 aatattttca aggtaaaaca aacaatttca aacaaaaaca aatgttagat gatgaaataa
     1561 gaacagtgga ttgacgtata ttagcttagg tcagattttg tataagacga aaataaagta
     1621 ggacctctta atcagtaagt tatagaaagt aaaagacttt tgtaatactt gaatagatat
     1681 ttcacgtcca ttttgtgatg gattaaatga acaaaaatga acaataattt aacggtgtta
     1741 tctatttttt aagaaaacaa ataaaaaaaa caaaaaataa acaaaaaagt gttgcttttt
     1801 gtttgaatga ttgatattat ataaacaaag aaatgatgaa aacgtttttt cgaacatttt
     1861 gcaaaatgtt ttctacttct acgtagcatt tctttctaaa atttaggagg tggtcaaaat
     1921 ggctattgtt gttggtgcag atctcaaagg tacgagatta aaagatgttg tgaaaaattt
     1981 tcttgtagaa gaaggttttg aggtaattga tgtgactaag gacggacaag attttgttga
     2041 tgttaccttg gcagttgcct ctgaagtaaa taaagatgag caaaacttag gtattgtaat
     2101 tgatgcttat ggagctggtc cattcatggt agcaactaaa attaaaggta tggttgcagc
     2161 agaagtttca gatgaacgct cagcttatat gacacgtgga cataacaatt cacgaatgat
     2221 tacggttggc gctgaaatcg tcggtgatga gcttgctaaa aacatcgcta aaggtttcgt
     2281 caatggtaaa tatgatggcg gacgtcatca aatccgagta gatatgctta acaaaatgtg
     2341 ttaagaaaag gagttaagga atgagaatcg caattggatg tgaccacatt gtcacagatg
     2401 aaaaaatggc agtatcagaa ttcttgaaat caaaaggaca tgaggtcctt gatttcggga
     2461 catatgacca cgcacgtact cactacccta tctatggtaa aaaagtcggt gaagcagtag
     2521 taagtggtca agcagacttg ggagtatgta tctgtggtac aggtgtcggc attaacaatg
     2581 cagtcaataa agttccaggt gttcgctcag cgttggttcg tgatatgaca tcagctatat
     2641 atgcaaaaga agagttaaat gcgaatgtta tcggtttcgg tggaaagatt actggtggtc
     2701 ttcttatgaa tgacatcatt gaagctttca ttgaagctga gtacaaacca acagaagaaa
     2761 ataaaaaatt gattgcaaaa atcgaacacg ttgaaacaca taatgcacat caagcagatg
     2821 aagatttctt tacagaattc cttgaaagat gggaccgtgg tgactaccac gattaaggta
     2881 taacaaatga ttctgacagt cacactaaat ccttcagtag acatctctta tcctctagaa
     2941 acattgaaaa ttgatacagt gaatagggtg aaagatgtta gtaagacagc tggtggaaaa
     3001 ggcttaaatg ttactagagt cttgtatgaa tctggtgata aagttactgc tacaggtttc
     3061 ttaggtggta aaattggtga atttattgaa agcgaattag aacaatctcc tgttagtcca
     3121 gctttttata aaatctcagg aaacactaga aactgtattg ctatcttaca cgagggtaac
     3181 caaacagaaa ttttggaaca aggaccaaca atttctcatg aagaagcaga aggctttctt
     3241 gaccactata gcaatcttat taaacaatca gaagttgtga caatttcagg aagtttacct
     3301 tcaggacttc ccaacgatta ttatgaaaaa cttatccaac ttgcttcgga tgaaggagta
     3361 gcagtcgttt tggattgttc aggtgcacct cttgaaacag ttctaaaatc gtcggctaaa
     3421 cctactgcca tcaaacctaa taatgaggaa ctttcacagt tgcttggaaa agaagtaaca
     3481 aaagatattg aagaactcaa agacgttctt aaagagtcac tttttagtgg tatcgaatgg
     3541 atagtggtct cattaggtcg aaacggtgcc tttgcaaaac atggtgatgt tttctataag
     3601 gtagatattc ctgatatccc agttgttaat ccagtcggat caggggactc aacggtagct
     3661 ggtattgcat cagctttaaa tagtaaaaaa agtgacgctg acttattaaa acatgcgatg
     3721 acattgggta tgttaaatgc tcaagaaaca atgacagggc atgtgaatat gactaattac
     3781 gaaacactaa actcacaaat tggagtaaaa gaggtataaa aatggtactt acagaacaga
     3841 aacgcaaatc tttggaaaaa atttcagata aaaacggtgt tatctcagct ttggcatttg
     3901 accaacgtgg tgctttgaaa cgtttgatgg cacagtatca agatacagaa ccaactgtag
     3961 ctcaaatgga agaactaaag gttttggttg ctgatgaact tacaaaatac gcttcttcaa
     4021 tgttgcttga ccctgaatat ggacttccag caacaaaagc attggataaa gaagcaggtc
     4081 ttcttcttgc ctatgaaaaa acaggttatg atacatcaag tacaaaacgt ttgcctgact
     4141 gcttggatgt ttggtctgca aaacgtatca aagagcaagg cgctgatgcc gttaagttct
     4201 tgctttacta tgatgtagac agctcagatg aacttaacca acaaaaacaa gcttatattg
     4261 aacgtgttgg ttctgaatgt gtcgctgaag atattccttt cttcttggaa atccttgctt
     4321 atgatgaaga aatctcagat gcaggttctg ttgaatatgc aaaagtgaaa cctcgcaaag
     4381 tcattgaagc gatgaaagtt ttctctgatc cacgctttaa cattgatgtt cttaaagtag
     4441 aagtaccagt taacgttaaa tatgtcgaag gttttgctga tggtgaagtg gtttacagta
     4501 aagctgaagc tgctgacttc tttaaagcac aagaagaagc gactaacctt ccatacatct
     4561 acctaagtgc aggtgtatct gctaaattgt tccaagaaac attgcaattt gctcacgact
     4621 caggtgccaa gtttaatggt gtgctttgtg gacgtgctac ttgggcagga tctgtagaac
     4681 cttacatcaa agaaggtgaa aaagcagcac gtgaatggtt gcgtactact ggattcgaaa
     4741 atattgatga acttaacaaa gttcttgtta aaacagctag tccatggact gataaagtat
     4801 agtaaaaaca taaaacggag gatattgtta tgaacagaga agagatgact ctcttagggt
     4861 ttgaaattgt tgcttatgct ggagatgctc gctctaaact tttagaagcg cttaaagcgg
     4921 ctgaaaatgg tgatttcgct aaggcagata gtcttgtagt agaagcagga agctgtattg
     4981 cagaggctca caattctcag acagctatgt tggctcgaga agcttctggg gaggaacttc
     5041 catacagcgt taccatgatg catggtcagg accacttaat gactactgtc ttattaaaag
     5101 atgtgattca tcacctcatc gaactttata aaagaggagc aaaataatgc ataaactcat
     5161 tgaacttatt gagaaaggga aaccgttctt tgagaaaatc tctcgaaaca tctatcttcg
     5221 tgctattcgt gatggattta tcgctggtat gccagtcatc cttttctcat ctatctttat
     5281 ccttattgcc tatgtcccaa atgcttgggg attccactgg tcaaaagaca ttgagacctt
     5341 cttgatgact ccttatagct attcgatggg tatcctagca ttctttgttg gtggtactac
     5401 cgctaaagct ttgacggact caatgaaccg tgacctacct gcgaccaatc agattaactt
     5461 cttatctacg atgctagcgt ctatggtagg gttccttttg atggcagctg agcctgcaaa
     5521 agaaggaggc ttcttgacag ccttcacggg aacaaaaggt cttttgacag catttattgc
     5581 agcctttgtg acagttaatg tttacaaggt ctgtgtgaaa aataatgtta ctattcgtat
     5641 gcctgaagag gttccaccaa atatctctca agtatttaaa gacttgattc cattcactgt
     5701 agcggttgtt ctcctttatg gatttgaact cattgttaag ggaactcttg gtgtaaccgt
     5761 tgcagaatca atcggtaccc ttcttgctcc tctcttctca gctgcagatg gttaccttgg
     5821 aattacactt atctttggtg cttatgcctt cttctggttt gttggtatcc acggtccttc
     5881 aattgtcgaa ccagcaatcg ctgctatcac ttatgccaat atcgatgcca acttgcatct
     5941 tatccaagct ggacaacatg cagataaagt tatcacttct ggtactcaaa tgtttattgt
     6001 taccatgggt ggaacaggag ctacattgat tgttccattc ttgttcatgt ggatttgtaa
     6061 atcagaacgt aaccgtgcca tcggacgtgc ctcagttgtt ccaacattct ttggggttaa
     6121 tgagccaatc ttatttggtg caccaatcgt attgaatccg attttctttg taccgttcat
     6181 tttcgctccg attgtcaacg tttggatctt taaattcttt gttgacacat tgaacatgaa
     6241 ctcattctct gccaaccttc catgggttac tcctggtccg ttgggaattg tactcggtac
     6301 taacttccaa gttctatcat ttatcttagc cggactcttg gtagttgttg atactatcat
     6361 ttattaccca tttgttaagg tatacgatga acaaattctt gaagaagaac gttctggtaa
     6421 aacaaacgat gctcttaaag aaaaagtagc tgcaaacttc aatactgcta aagcagatgc
     6481 cgttcttgga aaagcaggcg ttgctaaaga agatgtagca gcaaacaata atatcacaaa
     6541 agaaactaat gtccttgttc tttgtgcagg tggaggtaca agtggcttgc ttgccaatgc
     6601 cttgaacaaa gcagcagcag aatacaatgt tccagtcaaa gcggcagcgg gtagttacgg
     6661 tgctcaccgt gaaatgttgc cagagtttga cttggttatc ttggctccgc aggtcgcttc
     6721 aaacttcgat gatatgaagg ctgaaacgga taaattgggc attaaacttg caaaaactga
     6781 aggagctcaa tatatcaaat tgacacgtga tggacaaggt gctcttgcat ttgttcaaca
     6841 acagtttgat taataaattt gtaaaagtta tttgagacga gattattctt tcagttcctt
     6901 ataatattgt taaccatatc attgcatttc atttccttgg tttccggaac tcagaatatc
     6961 gtctcaaatg ctttttttga aaggatttat agttatgact aaaacacttc ctaaagattt
     7021 tatttttggt ggtgctacag ctgcttatca agcagaaggt gcgactcaca cggatggtaa
     7081 aggcccagtt gcatgggata agtatcttga agacaattat tggtacacag cagagcctgc
     7141 aagtgatttt taccataaat atcctgttga tttagaatta gctgaaaaat atggcgttaa
     7201 tggtattcgt atttcaattg cctggtctcg tattttccca actggctatg gcgaagtaaa
     7261 tgaaaaaggt gttgaattct accataatct ttttgccgag tgccacaaac gtcatgttga
     7321 gccattcgtg actttgcacc actttgacac accagaagtt cttcactcag atggagattt
     7381 cttaaaccgc gaaaatatag aacactttgt agattatgcc gctttctgtt ttgaagagtt
     7441 cccagaagta aactactgga caactttcaa tgaaattggc ccaattggag atggtcaata
     7501 cttggttggt aaattccctc caggcattca atatgatctt gccaaagtct tccaatcaca
     7561 ccacaatatg atggtttctc atgcacgtgc tgtgaaattg tataaagata aaggttataa
     7621 aggcgaaatt ggtgtagtac atgcgttgcc aaccaaatac ccttatgatc cagaaaatcc
     7681 agcagatgtt cgtgccgctg agttagaaga tatcatccat aacaaattta tcttggatgc
     7741 aacctacctt ggacactatt cagataaaac gatggaaggg gtcaaccaca tcctagctga
     7801 aaatggtgga gaacttgatc ttcgtgatga agacttccaa gcacttgaag cagctaaaga
     7861 tttgaacgat ttccttggta tcaactacta catgagtgat tggatgcaag cctttgatgg
     7921 tgagactgaa atcattcaca atggtaaggg tgaaaaagga agttctaagt accagattaa
     7981 aggtgttggt cgccgaatcg caccagatta tgtgcctaga acggattggg actggattat
     8041 ttatcctgaa ggcttgtatg accaaatcat gcgattgaaa aatgattatc caaactacaa
     8101 gaagatttac atcactgaaa acggtcttgg atacaaagat gagtttgtag ataataccgt
     8161 ttatgatgat ggtcgtattg attacgtgaa gaaacacttg gaagttttat cagacgcaat
     8221 tgcggatggt gcaaatgtta aaggttactt catctggtca cttatggatg tcttctcatg
     8281 gtcaaacgga tacgaaaaac gttatggact attctatgta gactttgaca cgcaagaacg
     8341 ctatcctaag aaatcagctc attggtataa gaaagtagca gaaactcaag tgatagagta
     8401 atattacttg aaaatgtaaa aaattgttat ccgatacttt agaaaaagta caacacttat
     8461 ttagaccagg ctttgaaaag gtatatattg ttagtttcga agattgtcct atgattcctg
     8521 agcttgaagc taccctcgtt gctaaaatgt ggtaaatggt atgttttaac tggaaaagag
     8581 tggatttgcc attctgattt agagttatca gcatttgaat gggaattttt acaatcactc
     8641 gatgtagaaa tacgtgagac tattcacttc gaggtaaatt atttgccttt ccaataataa
     8701 aaagaaagga attagttgtg actatcgaat taaaaaacga gtatcttact gttcagttta
     8761 aaacacttgg tggtcaactg acttcgatta aagacgagga tgggatagag tatctctggc
     8821 aagctgatcc aaattattgg aatggacagg cgcctattct atttcctatc tgtggtagtt
     8881 tacgaaatga ttgggctatt tatagacctc aagaaagacc tttttttaca ggtcttattc
     8941 gtagacatgg ttttgttcga aaagaagaat ttattctgga agaagtcaat gataatagcg
     9001 tgacctttag tattaaacca aatgctgaga tgcttgataa ttacctatat cagtttgaac
     9061 tgagagttgt ttataccttg aatggtaaat caattagaac cgaatttcag gtaacgaact
     9121 tggaaatgga aaaaacaatg ccttacttta ttggagccca tccagcattc aatagttctt
     9181 tagtagaagg tgaaaactat gaggattatt atattgaatt tagtgaggtt gagtcttgct
     9241 cgataccaaa gagttttcct gagacaggat tattagattt gcaagatcgg acaccttttt
     9301 tggaaaatca aaaaattctc gatttggact actcactttt ttcccatgat gccattacac
     9361 ttgaccagct caagtctcgt agcgtaacgc ttcggtcaag gaagtctggt aaaggtcttc
     9421 gagtagattt tgatgatttt ccgaacttga ttctgtggtc tacaactaat aaaagtccat
     9481 tcattgcttt ggaaccttgg agtggtctct ccacttcact tgaagaaggt aatttcttag
     9541 aagataaaag acaggtaacg aaaatttcac ctcaagaaac ttctagaaaa atctatgata
     9601 ttactatcct taattaaaat ttgttattat ttgagtcttc aataagattt gaatactctt
     9661 accttgtgat gatagattgg tcttctccgt gccagtctta catcgcaagg tttttaagtt
     9721 ttttgtgggt ctatgtcaac tgtagtgggt gacgaaaagc taatatctaa agacagtctg
     9781 gtagtaaaaa ataagattat ttactacttc ttgatttaaa ataatagtgg agccattaat
     9841 ttgtggcaaa tttaatttaa tattttgcct caaattagcc acacttttta tagaactaag
     9901 aggaaaaatg aaattttctt cctattaata tagttgtttg aaatcttaaa tatagcgt
//
LOCUS       DBNIBO010000103         9948 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.107, whole genome shotgun sequence.
ACCESSION   DBNIBO010000103 DBNIBO010000000
VERSION     DBNIBO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.107"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="AC1Y77_002034"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="AC1Y77_002034"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIQ1575592.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="AC1Y77_002035"
     CDS             complement(1173..1868)
                     /locus_tag="AC1Y77_002035"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIQ1575593.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="AC1Y77_002036"
     CDS             complement(1881..2597)
                     /locus_tag="AC1Y77_002036"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HIQ1575594.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="AC1Y77_002037"
     CDS             2927..3532
                     /locus_tag="AC1Y77_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIQ1575595.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="AC1Y77_002038"
     CDS             3857..4180
                     /locus_tag="AC1Y77_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HIQ1575596.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="AC1Y77_002039"
     CDS             4362..5732
                     /locus_tag="AC1Y77_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIQ1575597.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="AC1Y77_002040"
     CDS             complement(5862..7163)
                     /locus_tag="AC1Y77_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIQ1575598.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="AC1Y77_002041"
     CDS             complement(7393..9384)
                     /locus_tag="AC1Y77_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIQ1575599.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="AC1Y77_002042"
     CDS             complement(9528..9794)
                     /locus_tag="AC1Y77_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIQ1575600.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2912 a         2068 c         1804 g         3164 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaa
//
LOCUS       DBNIBO010000104         9739 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.108, whole genome shotgun sequence.
ACCESSION   DBNIBO010000104 DBNIBO010000000
VERSION     DBNIBO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.108"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            180..665
                     /locus_tag="AC1Y77_002043"
     CDS             180..665
                     /locus_tag="AC1Y77_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIQ1575601.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            658..1341
                     /locus_tag="AC1Y77_002044"
     CDS             658..1341
                     /locus_tag="AC1Y77_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIQ1575602.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1467..4112
                     /gene="polA"
                     /locus_tag="AC1Y77_002045"
     CDS             1467..4112
                     /gene="polA"
                     /locus_tag="AC1Y77_002045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIQ1575603.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4157..4993
                     /gene="mutM"
                     /locus_tag="AC1Y77_002046"
     CDS             4157..4993
                     /gene="mutM"
                     /locus_tag="AC1Y77_002046"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HIQ1575604.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4957..5586
                     /gene="coaE"
                     /locus_tag="AC1Y77_002047"
     CDS             4957..5586
                     /gene="coaE"
                     /locus_tag="AC1Y77_002047"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIQ1575605.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5638..5970
                     /locus_tag="AC1Y77_002048"
     CDS             5638..5970
                     /locus_tag="AC1Y77_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIQ1575606.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6282..6773
                     /gene="nrdR"
                     /locus_tag="AC1Y77_002049"
     CDS             6282..6773
                     /gene="nrdR"
                     /locus_tag="AC1Y77_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIQ1575607.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6796..8169
                     /locus_tag="AC1Y77_002050"
     CDS             6796..8169
                     /locus_tag="AC1Y77_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HIQ1575608.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8169..9095
                     /gene="dnaI"
                     /locus_tag="AC1Y77_002051"
     CDS             8169..9095
                     /gene="dnaI"
                     /locus_tag="AC1Y77_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HIQ1575609.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9224..9664
                     /locus_tag="AC1Y77_002052"
     CDS             9224..9664
                     /locus_tag="AC1Y77_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HIQ1575610.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         3363 a         1703 c         2146 g         2527 t
ORIGIN      
        1 caaatcaaaa acatgagaag ctgttttttc ttggctgttc ttattatttg atgcaggaca
       61 gctttttcac gacctctgta aactatagga atctgttaaa tcaagggagc agaacttgcc
      121 ttttacgcta cttttggtac aataatgtat gattaaaaat aaactggagg gtttctatct
      181 tgaacatagg taagccgaga aaactaggaa aaacaataaa tgaaatcgag gaaggcgact
      241 cgttatcctt aacagaatct attgaagata accagctttt gctgtatttg ggattgacga
      301 atgatgcaaa tcctttgtat atccaacatg actttgcaca aaaaacagaa tatcaacagc
      361 caatcgttcc atcagtgatg ctgatgggga tcatcacaag tgcaatttca aagcatcttc
      421 ctggaccagg atcacatgtt gtcaattttt ctgtcaattt tattgaaccg gttttccatt
      481 atgaaacact gacttttcag tttgaggtca taaaagtaga taagatgaag gatgtggtga
      541 cgatttctgt agaagcggag aatacgaacg aagatcgtgt gttggatgct gtcgttatgg
      601 tccaaccgcc acaagttcct gaattggaaa tagcaatcga aagtgaaggt gaattgaatg
      661 aataatccaa gtacatcaat gtcaggtatc aatcgttttt atgcaaaagt ttatggattt
      721 ttaggtatgg gacttgccct tagtgcagtc acttcttact tggctctttg ggtatttcct
      781 ttgcaattag caacttttat caataatttt ccactaggct ttgtgggtct gtggatcgtt
      841 gaactgattt tagtaatggt tctaggcgta aaagcacaaa agaatccgag cttagcaatc
      901 ggtggattta ttgcctactc tcttttgaat ggcataacat tagcggttac tcttgcatac
      961 tacgatattg gaacagtaac gcaagctttc ttgacagcta ctttgatgtt catggggatg
     1021 tctgttgtcg gagtaacaac aaaaagagat cttagcgggg ttggaagagc aggacaggca
     1081 gctttaatcg gagtgatcat tgcactagtc ttgaacatct tcattttgag aagtagcgta
     1141 gttgagctgt tcatctccct actgttagtc gtcatctttg cagggattac agcttatgat
     1201 aaccaaagca tccgtaatat ctatgtccag acaaacggaa cagcacatag tggaattgct
     1261 gtattcatgg cattgaagct gtatttggac tttatcaact tgttcttggc gtttctgaga
     1321 atctttggac ggaacaacta aaaaagaaaa aaattcatca gttggggcta taccgaatta
     1381 tttggtatgg tcccgctttt tcttttcgca ttccgcagga ttctttgtta gaatgaataa
     1441 gaactttaca gtaaggagtt aagaagatga caaaaaataa attattactc gttgatggga
     1501 acagtgtggc ttttcgcgca ttctttgcgc ttcacaattc tcttgaacgt ttcaaaaata
     1561 gaaacggtct tcatacaaat gcaatctatg cattcaatac gatgtttgaa aatgttatgc
     1621 aaaaagagca accgactcat gttctggttg cgttcgatgc aggaaaaacg acttttcgga
     1681 cagaaatgta tgcggagtac aaaggtggaa gatccaaaac acctggcgaa ttcaaagaac
     1741 aaatgccata tatacgtgat ttgttgacag gactaggggt acagtattac gaattgccca
     1801 attatgaagc agatgatatt atcggtactt tggcagaaaa agtagacaaa gatcaatttg
     1861 atgtcgtcgt gctttcaggt gatcgggatt tgactcaatt agctagtaaa gaagtaaaag
     1921 tggatatcac tgtaaaaggt gtcagcgata tcgaaagcta tacaccagaa catgtagcgg
     1981 aaaaatatga tgggttgacg ccaaaacaga ttattgacat gaagggacta gccggagatg
     2041 cctctgacaa tatccccggt gtgactaaaa tcggtgaaaa aacagctatc aagctattga
     2101 aacaatatgg ctcagtcgaa ggaatttatg aacatatcga tgaaatgaag caaagcaaaa
     2161 tgaaagaaaa tctgataaat gacaaagagc aggctttctt atcgaaaaaa ttggcgacca
     2221 tcaatacttc agcgcctgtg gatgtctcta tcgactcatt gaagtatgaa gggaaaaatc
     2281 tcgataaact cgttccgttt tataaagaaa tggatttcaa tcagttcctt tctaaattga
     2341 atatcgtaga agaagaagta aaaatggatg atattttgtt tgaagtcgtt catgaattca
     2401 aagaggaaat gtttactacg gatatggcgt tgtacgtgga aatgatgggc gacaattatc
     2461 atacagagga aatcgtcgga gtagcgtggg ggacagaaaa gaaaatttat gtcacgaacg
     2521 atctatctgt attcgaaaac cgagcgttcc attcttggat cactgatccg actcggctga
     2581 aaaaagtata tgatgcgaaa cgaacttatg ttgctttaaa ccgctatact ggaaaaacga
     2641 aaggaatcga ttttgatgtc ttgctaggtg cataccttct cgatacgaat gaaaaaagca
     2701 cagatatcga aggaatagct gctcattacg gatacaacga tatccaatca gatgaagctg
     2761 tatacggcaa aggggcgaaa aaaggtttac cagaagaaga ggaactcttt tttgctcatt
     2821 tagcgcgaaa agtagctgca atcaatgcgt tgacaccaaa attgagccaa gaactggcag
     2881 aaaaaaacca agaagatctt ttccggaaaa tggaactgcc attgtctatc attttggcag
     2941 agatggaaat aagcgggatc aaagtagatg cgactcgctt acaagaaatg aagggcgagt
     3001 tctctgctcg cttaagagag atcgaacaaa aaatctacga agaagccggc gaagaattca
     3061 atttgaattc accaaagcaa ttaggagtga tcttatttga aaaaatggga ttacccgtta
     3121 tcaaaaaaac aaaaactgga tattccacag ccgtagatgt actggaacag cttcgtgaac
     3181 aagcaccaat cgtagaggat atcctcacct atcgtcaaat tgcaaaaatt caatctacct
     3241 atgtcgaggg actgctgaaa gtgatccaat cagatggaaa agtgcatacc cgttatgtcc
     3301 agacattgac ccaaacagga cgattaagct ctgttgatcc taatttgcag aacatcccga
     3361 ttcgattaga tgaagggaga aagatacgtc aggcatttgt tcctagagaa aaagactggc
     3421 tgatttattc ttctgactat tcacaaatcg agttacgcgt acttgcgcat atctcaaatg
     3481 atgaacattt gaaagctgcc ttcctagaag gtcaagatat ccatgccagc acagcgatgc
     3541 gtgtatttgg aatcgaaaag gcagaggatg tcacaccaaa tatgcgtagg caggcaaaag
     3601 cagtgaattt tggtatcgtg tatggcatct ctgattacgg actttcacaa aatctaggaa
     3661 tcagccgaaa agcagcacag caatacatcg atacgtactt tgaaaaatac ccaggtgtca
     3721 aagaatatat ggaaagaatc gtacgcgaag caaaagacca aggctacgtc gaaacgctgt
     3781 atcaccgccg tcgatatctg ccagacatca attcaagaaa ttacaatatc cgttcttttg
     3841 cggaacgaac agctatcaat acaccaatcc aaggaagtgc tgctgatatt ttgaaaatcg
     3901 cgatgatcga attggataaa cgattaaaag aaacaggtct tcaagctacg atgctgttac
     3961 aagttcatga tgaacttgtc ttcgaggtac cggaaaaaga acttgaatca ttagataaat
     4021 tggtgaagga agttatggaa caagctgttt ctcttcatgt accactgatc actgacagca
     4081 gttggggaaa aacgtggtac gaagcaaaat aaacattgtt taagacagag caatataatg
     4141 aaggtgaaaa gaaaaaatgc cagaattacc agaagtagaa accgttcgta aaggattgga
     4201 acggttagta gtaggaaaaa cgattcaaaa agttcaggtc ttgtggccca agatcatcga
     4261 gcaaccagag acacccatct ttgaggcttc tcttgttggc gaaaccatcc aatccattgg
     4321 acgtagaggg aagtttttaa tttttcatct cgatcattgt gaactgatct cacacttgcg
     4381 aatggaagga aaatatcaat ttacgaaaga aaatacacca atcgataaac atacgcatgt
     4441 acttttcttt tttgaggatg gaagccaact tcgctacaac gatgtacgaa aatttggtcg
     4501 tatgacaatc gtcgaaaaag gagcaagtgc aacttaccga ggaatcatga aattgggacc
     4561 tgagcctttg ccagatagct tcttattagc agatttcgca aatggactga aaaagtccca
     4621 caaagcaatc aagccattgc tgctagacca acgtctagtg actggtctag gtaatattta
     4681 tgtagatgaa gcattatggg aagcgaagat ccatccagaa caaccggcga acacactgcg
     4741 ccccaaagag gtagaacaat tacgtctatc gatcattgat gttttggatc gtgcaataga
     4801 agccggaggt accacgattc gcagttattt gaatgcatta ggagaatcag gcggatttca
     4861 agtagcactg catgtctatc agcaaactgg caaaccatgt atacgttgtg gaacgccgat
     4921 cgtgaaaaca aaagtggcac aaagggggac gcactattgt ccgaattgtc aaaggctaaa
     4981 aatcgcaaag taggatttgt tttaggtttg acgggtggaa tagctaccgg taaaagtacg
     5041 gcagccaaag tctttcagtc tcatggattt cctttgatcg atggcgatgt gattgccaga
     5101 gaaactgttg agcctggaac gcttgctctg aggaaaattg tatcagtgtt tggccaagag
     5161 gtattgcagc ctgatgggca actagatcga gggaaacttg ggatgattgt ttttccatca
     5221 aaggaaaaac ggcaaaaact tgatcagcta ttagatccgt ttatccgaaa agcgataaaa
     5281 gatcaaatcg cctctctaag ttccaatcat cctttagtaa ttgtagatat cccattgctc
     5341 tacgaaggtc attatgacca ctatatggat gcagtagctg tcgtttatac tacgccagaa
     5401 actcagcttc agcgtttgat gaaacgtaat caactgacgc tagaacaagc gcagcagaga
     5461 attagtagtc aacttccaat cgaagaaaaa aagcagcgtg cggatatttt gttcgacaac
     5521 aatggaacaa aagaaaaact tgtgggacaa atcgaaaatt ggctttttgc taatcgcttt
     5581 ctttgagtat ctgtgtcaat tttgctatcc tgagtatata gaaagtgagg gatagcgatg
     5641 ggatcattaa aaaatcaaaa ggataaagtc gtaggtaacg caaaagaaaa aatcggcgag
     5701 atgctcaata aagaaaatct aatcgatgaa ggtcgtacgc aatcagaaac agcagaagaa
     5761 aaagaaaagc agtaccaagc aagaatcgat gaaatagaag accagcaggc agaagaagaa
     5821 gcgaagcggc aagatttgac tcaagaacga aaaaatccga cacctgatcg tagtaaaaca
     5881 ccgctcccag aaagtgacca cattcccgat gagcaaaaaa tcgaggaaca gcgaatgaaa
     5941 cactatacag gaatcgaaga gaagttataa aatagtaaaa aatcgagatg tgacttgaag
     6001 cagatgagaa ggtgttcatc tgcttcaagt tttttgtgct agatgactaa atcagctttt
     6061 gtgtaagtta tttctcagta atcaaggtga tcagtattgg aaaattgaaa tttagtaagg
     6121 acaggggaca caattacgca tgaaagtaga aaaaattgtt cttatatccg tgttttttgg
     6181 gtaggacagt cgcgtttgtg atttgtttcg tgttataata caactagttg taaagaaaga
     6241 cacatccttt atataaaatg cgatgcaaga ggtgagacaa aatgagatgt cccagatgtc
     6301 aacataataa ttcacgagta atcgacagcc gtcaagcaga tgatggacgt gcgatccgtc
     6361 gccgtcgcga atgtgagaat tgttcgttcc gattcactac atttgaacga attgaagctg
     6421 ctccgttatt agtaatcaag aaaaacgggg aacgcgaaga gttcaatcgc gataaaattt
     6481 taagaggact catccgttca gcagaaaaaa gacctgtagc tatggaacaa atggaacaaa
     6541 tcgtcaacca tgtcgaaaac cgcgtacgca gtctcggaga aaacgaagta tcgacgaatc
     6601 taattggtga atatgtgatg gaagatttag ttgatttaga cgaaatcgct tatattcgtt
     6661 ttgcaagtgt ttatcgccag ttcaaagata tgtctgtttt cttaaaagag ttgcaagata
     6721 tcgtggataa agcaaaagaa aataataaag agaataataa agaaaacaat taagaaacaa
     6781 aaaggaggca tttccttgga aaatgcttgg aaagagcttc aaccaaaaaa tatttaccaa
     6841 ggaagaaaag cggctccgct gagagaagac agcaagaacg ccttgctgta tctttatcag
     6901 ccactaatcg gcggagaagc cttgagtatc tatttcaccc tgttatcaga aatctctcaa
     6961 gaatctggca aaggtcctga agggatgcat gcagatctgc ttagtagtct aggctgcggt
     7021 atcccgcaat tttatgatgc aagaaaaaag ctagaaggaa tcggcttact ggatgtttat
     7081 tttaaagaag atagtgatct gggacctcgt tttatttatg agctgttaga accactcatt
     7141 cctaaagcat ttttcaaaga ctctgttctg gcttttcttc ttctggaaaa agttggtgaa
     7201 cgacgattcc agaagcttac ccaattcttt gaacctaaaa ggcatacagc agagggatat
     7261 caaaaaatca caaaaaaatt tttagatgtt tatcgcttca atgaagcagt gtacgctgct
     7321 aatcaagaaa acatcgaatc gctgaataaa tcttttgaag aatctgaatc agtagcatta
     7381 cttgaagcag attcgacatt cgattggcag tttttgaatg attggctggc aaaaaaacat
     7441 attgcaagtc ttgatgatcc aatcatcaaa caagtcgaca tgtatcatca gctttacggt
     7501 ttcgatgaat tgacacttgg cgaactgatc agtcaatctt atgactttag tgaacaaaaa
     7561 gtatctgaaa aagaactgca aaaagccatt ttggcaatgc agagacgtcc ggtgaagcaa
     7621 acaaaagaaa cagttgcggt ttctgaaaac agtaatgaac cctcgtcaaa cgaacatttg
     7681 acaaacgcag cgcttcagct gataaaagaa gcacagagca ttccaccgat gaaatatctg
     7741 gaagcaatca agtcagaaaa aggcggatat gtttctaaac aagaaaactg gttactgcaa
     7801 gatcttgttt ctcaatcagg tctttccagt agcgtgatca atgttttgct gaattatgta
     7861 ctagtgatca agaataatgc tagcttaaat gccagttatg taaatacagt ggcaaatgaa
     7921 tgggcacaaa agaaaatcgc cacagcagaa gaagcgatcc agcacattcg gcaaataagt
     7981 aatcaagcaa aacaatcaaa acaaaagaag caaacaaatt atcaaactgg caagcgaaat
     8041 gttcgacggg aaaagcttcc tgattgggtc aaccagccaa aagatgaaaa acagatcagc
     8101 agcgagaaaa aagcagagat tgaccgccgt tttaaagaat acctggcaaa aaaggagggt
     8161 gatatctagt ggaagacgta ggcaaagaat tgaagaggat catcagcaaa cgcaattatc
     8221 aagaaaagta tgctgaaatg atcgctgaag tcttaaatga tgcggatgtt caagcttttc
     8281 taagcgcaca ccaagaagaa ttgacacaag cggatattga aaaaagttac gctaaattgt
     8341 atgaattcgt gcaagaaaag cgtaaatatc aaaaaaacga tccaacgatg atcgctcccg
     8401 gctatgagcc gaagctgacg ttgaattttc attatgtgga tgtcacttat gtaccaacgg
     8461 aagcattgtt agcacggcaa agacaagaag aaatcagaag ccgagtaaaa gcaatggata
     8521 tgccaaaaga tattcaatcg gcttcttttg aaaactttga acgtacagat ggacgaggat
     8581 atgcgagctt agaagcccta gattttgtag agaaatatac tgctgatcca aaagggtatc
     8641 ataaaggact gtatctagta ggaagttttg gtattggaaa aacatattta ttaggagcaa
     8701 cagcgagaga attagcgatt gctggattca ccacgacgct agtccatttt ccgacatttg
     8761 ccgttgagat gaaacaggca atcggaaaag atcaagtggc agaaaagtta gatgcagtga
     8821 aacgttcacc tattttgatg atcgatgaca ttggtgcaga tgcgatgagt agttggatac
     8881 gagacgaagt attcggtgtt atccttcaat atcggatgca ggaacagtta ccaaccttct
     8941 tctcatcgaa ttttacaatg gatgaattgg aacagcattt gtccgtcact caacgtggag
     9001 atgaagagcc gctaaaagcg aaacggataa tggaacgcat acgttatttg acaaaagaaa
     9061 tcgaaatgac tggtcgcaac cgacgcaatg gttgaacagt tgacaagtcc aactgacatt
     9121 tgacctagta gtaaaagcag gtcagcgaat cattatatgg acagaaatga ccggagacaa
     9181 tcaccgcaac aagtaatgca gaaacttaaa cggaggattt tttatgaggg ctattttttc
     9241 tagtatatct gtagttggtc tattttttct tttagcaggc tgcggtagcg cagatagcaa
     9301 cacaggttct gttgattcga ttgatgcggg tcaaacagta aaaacaagca gtacgaaaac
     9361 ggagagacaa agcatatttg tcggaacgat tgaaagtatc actcaagtag aagcagaaga
     9421 agaaacgctt cagttgaaac tcgtcgatgt catggccaaa gaagatccag cgaatatggg
     9481 aacgtcattc agcaatgacg gagtgatctt aaatgctact gctgaaaaaa taagcgtgga
     9541 cataaatgaa ttgaaaatcg gtgacaaagt ccaatttaca ttagttgaga tgccgatcat
     9601 gacgatgtct atcccaccgc aagttcctgg agattcgatt attgatgttt cattgaacaa
     9661 ataaccaata acattttttc aaaaataaga aaatgttgtt ttatagaacg aaaaggtata
     9721 ttttttaagg agatatggg
//
LOCUS       DBNIBO010000105         9485 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.109, whole genome shotgun sequence.
ACCESSION   DBNIBO010000105 DBNIBO010000000
VERSION     DBNIBO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.109"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..735
                     /locus_tag="AC1Y77_002053"
     CDS             <1..735
                     /locus_tag="AC1Y77_002053"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIQ1575611.1"
                     /translation="TEVYSVLLDSTMQGEVAHRYMLGLYEFLEKLTTNYPTILFESCS
                     GGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVYPISSMGAHVSAVPNHQTGR
                     ETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAFYKKHRQLLQYGTFYRLESP
                     FEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLAGLAEEATYQMEDVTFAGDE
                     LMNLGFYIDSELHGDYATQRFYLKQI"
     gene            796..2016
                     /locus_tag="AC1Y77_002054"
     CDS             796..2016
                     /locus_tag="AC1Y77_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIQ1575612.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            2013..3473
                     /gene="gtfA"
                     /locus_tag="AC1Y77_002055"
     CDS             2013..3473
                     /gene="gtfA"
                     /locus_tag="AC1Y77_002055"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HIQ1575613.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            3550..4656
                     /locus_tag="AC1Y77_002056"
                     /pseudo
     CDS             3550..4656
                     /locus_tag="AC1Y77_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123848.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            4978..5187
                     /locus_tag="AC1Y77_002057"
     CDS             4978..5187
                     /locus_tag="AC1Y77_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HIQ1575614.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            5180..5329
                     /locus_tag="AC1Y77_002058"
     CDS             5180..5329
                     /locus_tag="AC1Y77_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HIQ1575615.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            5483..6253
                     /locus_tag="AC1Y77_002059"
                     /pseudo
     CDS             5483..6253
                     /locus_tag="AC1Y77_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(6432..7517)
                     /gene="xerS"
                     /locus_tag="AC1Y77_002060"
     CDS             complement(6432..7517)
                     /gene="xerS"
                     /locus_tag="AC1Y77_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HIQ1575616.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            7851..8084
                     /locus_tag="AC1Y77_002061"
     CDS             7851..8084
                     /locus_tag="AC1Y77_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HIQ1575617.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            8081..8488
                     /locus_tag="AC1Y77_002062"
     CDS             8081..8488
                     /locus_tag="AC1Y77_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIQ1575618.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(8939..9448)
                     /locus_tag="AC1Y77_002063"
     CDS             complement(8939..9448)
                     /locus_tag="AC1Y77_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575619.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT         3261 a         1452 c         1733 g         3039 t
ORIGIN      
        1 acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat
       61 atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt
      121 gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa
      181 acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc
      241 ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca
      301 ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat
      361 gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc
      421 ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg
      481 tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc
      541 ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa attagcagga
      601 ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg
      661 aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat
      721 ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt
      781 ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat
      841 tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa
      901 ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc
      961 atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag
     1021 tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt
     1081 atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat
     1141 ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt
     1201 gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat
     1261 caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca
     1321 cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg
     1381 gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga
     1441 atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag
     1501 tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa
     1561 cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc
     1621 tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa
     1681 gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga
     1741 aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa
     1801 aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta
     1861 aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc
     1921 aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa
     1981 agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca
     2041 tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac
     2101 atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc
     2161 gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg
     2221 tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc
     2281 gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa
     2341 tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt
     2401 taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga
     2461 cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag
     2521 ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa
     2581 ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg
     2641 aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg
     2701 aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt
     2761 attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat
     2821 cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata
     2881 cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt
     2941 acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca
     3001 attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata
     3061 atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa
     3121 tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag
     3181 aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac
     3241 ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg
     3301 atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta
     3361 aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta
     3421 tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc
     3481 catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag
     3541 gactcataca tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata
     3601 aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt
     3661 aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa
     3721 gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat
     3781 gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg
     3841 ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag
     3901 cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag
     3961 tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg
     4021 gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc
     4081 aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat
     4141 taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt
     4201 aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt
     4261 ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta
     4321 gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc
     4381 tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag
     4441 cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag
     4501 aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg
     4561 aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc
     4621 tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac
     4681 ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc
     4741 agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt
     4801 ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt
     4861 aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa
     4921 cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg
     4981 gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga
     5041 atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga
     5101 agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta
     5161 gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt
     5221 tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg
     5281 gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag
     5341 aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac
     5401 cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa
     5461 aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa
     5521 gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat
     5581 ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa
     5641 agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt
     5701 tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg gttttatttg
     5761 tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa
     5821 agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc
     5881 caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga
     5941 cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat
     6001 gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat
     6061 gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag
     6121 tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa
     6181 acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag
     6241 agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac
     6301 acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac
     6361 acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt
     6421 atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta
     6481 tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa
     6541 tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga
     6601 attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc
     6661 ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat
     6721 cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg
     6781 acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg
     6841 tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca
     6901 ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa
     6961 gtatttttat attcattggc aatataatct agataatttt gtgtatcctt atctagaaat
     7021 aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct
     7081 acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc
     7141 gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat
     7201 agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt tacattttct
     7261 tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt
     7321 atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc
     7381 ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct
     7441 tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat
     7501 atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga gtatctctat
     7561 aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa atttattaat
     7621 ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata aatggtatac
     7681 ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc ttaattaatt
     7741 atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc ttgatttaaa
     7801 atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact atggcaatta
     7861 aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca aaggagtttc
     7921 ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa aaacttcgag
     7981 cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat
     8041 taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga
     8101 agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc
     8161 ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc
     8221 ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt
     8281 gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt
     8341 ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc
     8401 ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac
     8461 tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt
     8521 agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat
     8581 atagaaaacg taaattttct atatacttgg atatgctgga atgtttaaga gaataattac
     8641 atttctagaa aatacaataa aaattacagg ataattagcc atatcctacc tcctctagca
     8701 gttgctcaaa agttagcact aagtaacttc ttttttgttg ctggtaacaa tttatcaata
     8761 ctaaaatagc gtttaagtat ctaataaata ctaattttaa agctcttaat tattatgaaa
     8821 cacgatataa caatattgct atatcgtgct attgtaatat tatacatttg aagacagcgt
     8881 attcttgttc taatatcagg aattttttat aaatatcagt catattaaga ctcaaaattt
     8941 attggtatgt tcctctgtat agagtttgac cgtctaccat tatatagcaa ttattatcat
     9001 tgttgtcttg caccaatata ccgttaaggg tagcactagg agtagtaaca atcactggaa
     9061 actcatgaga aagctcggtc atttttcctg ttctgctaga atcgtgttct attgtcaagc
     9121 catttactat aaacgtgaat actttacttc ctattgttat agtagctttt tgagaaacag
     9181 tttctgtagc tggagctgtg cttctttccg aatttgttag ggtttcactg gattcattgc
     9241 tataaacaat tgtagaggat tctacggctt cttgagtgtc cacgttggtg ctgctagctt
     9301 catctagctt ccttttgtat tgttcgttac tgattgctaa gctttcccgt tggtcatcaa
     9361 gcttattcat ttctgtaatg gtataaaata atcctcctgc tagtgctata ttaacaacta
     9421 aactaaccgc aaataatacc tttttcattc ttccttctct tctttctctt tttgtaattc
     9481 cttac
//
LOCUS       DBNIBO010000106         9485 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.110, whole genome shotgun sequence.
ACCESSION   DBNIBO010000106 DBNIBO010000000
VERSION     DBNIBO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.110"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..242
                     /locus_tag="AC1Y77_002064"
     CDS             <1..242
                     /locus_tag="AC1Y77_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575620.1"
                     /translation="SFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGIS
                     ITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(407..1021)
                     /locus_tag="AC1Y77_002065"
     CDS             complement(407..1021)
                     /locus_tag="AC1Y77_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1575621.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            1471..2796
                     /locus_tag="AC1Y77_002066"
     CDS             1471..2796
                     /locus_tag="AC1Y77_002066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIQ1575622.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            2789..3139
                     /locus_tag="AC1Y77_002067"
     CDS             2789..3139
                     /locus_tag="AC1Y77_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575623.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            3121..3306
                     /locus_tag="AC1Y77_002068"
     CDS             3121..3306
                     /locus_tag="AC1Y77_002068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575624.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(4210..4485)
                     /gene="prgO"
                     /locus_tag="AC1Y77_002069"
     CDS             complement(4210..4485)
                     /gene="prgO"
                     /locus_tag="AC1Y77_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HIQ1575625.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(4457..5410)
                     /locus_tag="AC1Y77_002070"
     CDS             complement(4457..5410)
                     /locus_tag="AC1Y77_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1575626.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            6022..7515
                     /locus_tag="AC1Y77_002071"
     CDS             6022..7515
                     /locus_tag="AC1Y77_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HIQ1575627.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            7650..7946
                     /locus_tag="AC1Y77_002072"
     CDS             7650..7946
                     /locus_tag="AC1Y77_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HIQ1575628.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            8047..8898
                     /locus_tag="AC1Y77_002073"
     CDS             8047..8898
                     /locus_tag="AC1Y77_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575629.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            8919..9131
                     /locus_tag="AC1Y77_002074"
     CDS             8919..9131
                     /locus_tag="AC1Y77_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575630.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         3257 a         1483 c         1782 g         2963 t
ORIGIN      
        1 atagctttat gtatagagat ttttatgaag aatctataga attgactcca acggaaccaa
       61 ataaatctca aatcactttg tcaaaggagc tagagagaaa agtagagtct caagaactag
      121 ctataggaat tagtataacc aatgtgtcga catacaaaga attatacgaa gctaaatcac
      181 aagataataa ggattttgta gaaaatatag ataaggaacc aacaaataca gtaggggatt
      241 aacgtaaata agtgaagatt atattaacag actctttatt tagttaagta aaaaatggtt
      301 tgttggaatt ttatgcttta tctgttctga gtaagactta attttataga tagtaattat
      361 atgtaggaaa ttaaagatga gaagataata aaagcagcat gatcaactat ttgtcattga
      421 ccatgctgtc tttttttatt ctatacacgg tttgtctcgt aacattgtag tctttagcaa
      481 tcttagcaat cgcaacacca tttttaagat cttcaacgat gcttttgtaa acaaggcgac
      541 gttggggatc ttttgcgtct gcactgtata atttgggccg ccctttgtag acgccgtttg
      601 cttttgcgat cttaattcct tgtgcctggc gacgtttcga ttcagtccgt tcttgttcag
      661 cgatcatggc caaaatttgg atgatcagat ccttaatgaa tttatccagg agaggattgc
      721 caattgcttc agccatgatc ggtaaactag taatgatcag cttcacattt ttcttcttca
      781 agtaattgac tgaagcgatg atctcgtcat aatttcttcc aagtcgatca atagcttcca
      841 caataaaaat atcttgatct cggacgtaat ctaatgcttc ttggaaaaca ggtcgatgag
      901 taatcgttgc gccagattgt ttctcgatga aaattctctc tgcaccgaat tttttcattt
      961 cttcaatctg gcgttgttca ttttgatcga tcgatgagac acgtatgtat gcaactctca
     1021 aaacttttac ctactttctg caactattta aaatacaccc taatcataca cgaggaagcc
     1081 atatttttca atgttatacc agtgtctatt tagggtatac cttatataga caaaaactaa
     1141 tgatggagga aatagtatga gaattttttg taagtgtcta ctgataactt attctcaaat
     1201 gttgtaagga ttttattcat ttgttaatat taaagaacgg aactccaaaa aagtccgtac
     1261 tctaatttgc agtctctcac tcttttagag tgagagtttt tattagattt tcaaattaat
     1321 tagtaaagtg atagaataaa ttgatcatac aaatcaaaag aagaagcagg gtgatagcat
     1381 acctatgctt ctttttttat gtatttagct atttcttctt tacaatatcg aacatatgtt
     1441 tgtataatgt gaaagaatgg agtgttattt atgatggatt attcaaaaga gccagtaaat
     1501 gattactttt taatcgatat gaaaagcttt tacgcaagtg tcgaatgtat agaaagaaat
     1561 ttagatccat taacaacaga acttgttgtt atgagtcgag ctgataatac tggttcagga
     1621 ttgatattag cttcttctcc tgaagcaaaa aagcggtatg gtattacaaa tgtgagtaga
     1681 ccacgtgact taccacaacc atttcctaaa acactacacg ttgttccacc acgtatgaac
     1741 ctatatatca agcgaaatat gcaggtaaat aatattttca gaagatatgt ggctgatgaa
     1801 gatctactga tttactcgat cgatgaatca atcttaaaag tgacccgatc actgaatctt
     1861 tttacgactg aaggaacacg aagtcaacgg agaaagaagc tggctcaaat gatccaggag
     1921 aatattaaag aagagctagg attgattgct acagtaggtg tcggagataa tccccttttg
     1981 gctaaactag ccttagacaa cgaagcaaag cataatgaag gatttgttgc ggaatggacg
     2041 tacgaaaacg ttcctgaaaa agtatggaat attcctgaaa tgacggactt ttggggaatt
     2101 ggctctcgaa tgaaaaaaag actcaatcag atggggatta tgagcattag agatcttgct
     2161 aactggaatc cttatacgat taagaataga ctaggtgtga ttggcttaca gctgtacttc
     2221 catgctaatg ggatcgatcg aacagatata gcaatcccac cagaaccgac aaaggaaaaa
     2281 tcatatggaa attcacaagt gttaccacga gattatacta gaagaaatga aatcgagctg
     2341 gtggtaaaag aaatggcgga acaagtagcg atacgcatta ggcagcataa ctgtaaaaca
     2401 ggttgtgtcc atttacacat tggcacgtcg atcttagaaa cgagaccagg gttttcacac
     2461 cagatgaaga tccctatcac agataataca aaggaattgc agaattattg tcttttcttg
     2521 tttgataagt attatgaagg acaagaagta aggcatgttg gaataaccta tagcaagctt
     2581 gtgtacactg attcgcttca gctggattta tttagtgatc cacaaaagca aattgacgaa
     2641 gaaaatttag ataaaattat tgacaagatt cggcaaaaat atggttttac gtcgattgta
     2701 catgcttctt ctatgttaga aggcgctcgt agtattacta gaagtacatt ggttggtggc
     2761 catgcaggcg gcgcaggagg gcttaaaaat gattgattat aatgatcgtg gacgtttaaa
     2821 gtgggcaggt ttttatttat ctgatcatac tgaaaaaatt gatgccgaca atgaacaacg
     2881 agccaatcaa aatttggcaa aagaacaaat gacgactgaa gaggttgcgg aagtattgaa
     2941 cgctgcagtc ataaaaaata gatcagtatg tattcaaaaa gaagaacgaa acgctgaagg
     3001 atactatcca gaagatattg ttagcaagat cttaggtagt gatgagctgg ggatctacgt
     3061 tggaaaaaac aaagttcact acgacgaaat acgtcatatc gaattttacc aggataataa
     3121 atggggtcga ttagaatgaa cattatccag caatatgagc taaagtatat aacctttgat
     3181 cagctatcag aagagatttg gggttatggc cagcgtttga taaatgaggt tggtgttgaa
     3241 cgcttctctt tctatgtaga agctgcagca ggataccata aatacgaata ctatattggc
     3301 tattaaagtg caataaaatt atatttcttt tatgttttac attttgtatc aattctgata
     3361 aaattaccta taggtaatta aaaaatataa aaaaactagt tacaacctta ggacgttgta
     3421 actagcttga agcatactag agctaactaa aatagcacta gaaagcaatg ttaaacccgt
     3481 ccaacaaact tgatttaaca ttgagaccag cttattcagc taaatatagt tgaataagct
     3541 tcttttgttt taaaaaaaca aaaggtgtac tacgaataac gcaagtaaaa gaaatactat
     3601 tacaaccagt aggctacaag taagtattac taaacattgc gtagttagaa cctttgctct
     3661 attgtctaaa gccacaaaat tagagatcag ctttatgatg ctaagagcga gctgcatcca
     3721 tgcggacata ggttcttttg acactattaa ttctcctttt taggataagg gaaattgcta
     3781 gtagtttttt cattagcacc atcccccttc atttgattga gtctagtgct gctgtgatag
     3841 tttgctgttt aatatgctta cattcttatt ataacataaa tgaaacaaac acatggttag
     3901 aggtcgtatc aatcaaaata gtgtctcaca aacgatttat tacttactcc tgctgactaa
     3961 cggactgttc ttcagtaact gtaactatat gtttgttaaa accaccatta cctgtagata
     4021 aaaaccaata tatttactat actcactata ttaaatatat ttagtatagt aaaaactaga
     4081 aacaataaaa tttgtgaatg ttgtataccc ccaaacaata cacatatttt gaaggattag
     4141 gattaataaa gctaaaaata tagtaagtat actaaatata tttactatat ctagtatact
     4201 tactatattt taatcttttg atcgtctatt atatataatc cgttcctcgt cgtttaaagt
     4261 atcaacatag gcgtccattg caagtttgac tatttcatac ataggaatat cttttgcata
     4321 actaagatcc cgaatggttt ttagtacggg aggatctact tgaactggtt ttctttcttt
     4381 cgcagtgtac tttcgttccc ctaactcgac attaatatca tctttaattg aaatatcttc
     4441 agcaccaatt ttcttcttat ctttgttcgt tggtaataat gccatttagt ttaatctcct
     4501 ttccaagagg taggatatga ttggccattg agtcagcata tttaggtgta taatcaaatc
     4561 cttcaatatc accatgttgt tcatagtatt caattctttc ttctaattct gtaaagatgt
     4621 ctgcaaaaat agcccaccat cttcgatcta cataatcatt taattggata ccattctcac
     4681 catacatttc taaccgatcc gagcctttaa caatgctttt aaaaatgttt gattcaccat
     4741 acttatcaat tgttttttga tagtttgcgt gttgagagga acgtcgcact gagaaaagaa
     4801 caggcaaaat tccaatgatt tgaagatgag actcttcaaa attcgtgaca atcggaacta
     4861 aaactttatt aataaaatct tctgttcctt ccattgcgta ccgttttaat tcttgcattg
     4921 caataatata atctgcagca gttaagaatg cacgaaccac attatctgtt gatggtgggc
     4981 agtcgataaa gatataatcg aaattttttc tcaattcttc taaaggttta atgaaggcca
     5041 atgatctctt acgacgatca ggatattttt cggacaagta ttcattaagt tcttcagatc
     5101 caggagagcc agccataata tataaatgtg gacttacttg agtaatacct gaaacccagt
     5161 cccctttttt gattgctgca gacatggatc gatcaaagct agtaatttga tacgttgatc
     5221 caatgttgga agtagtattt ctttgttggt cataatcaat tagcaacacg tctttttcaa
     5281 aaagttgact agaaacaatc gctaataaat cattgtctgt agtcttacca atgccgcctt
     5341 ttctacctgt aagcattaca acttttccta gtttatctga tgttttaaat gcagtccgta
     5401 agttttccat agttccctcc aatttcctat actaaatata tttagtatac ttcatatact
     5461 taatatattc gatatatcta atatactcca aattgataag aattcagact ttataaggaa
     5521 tatagtggat atactaaata tatttgctat attcagtata tcagatatat tcaaaatgtc
     5581 aaaatatatt tgtgcgtttt gcgttgacaa tttagagtga aaagaggtaa acttaattct
     5641 atagaaacca cagataattc actatttaca aagtagcaaa agcagtggta ctctttaaac
     5701 agaaaaatta cataaaaaaa agagacggaa ataattgatt ttagcagaat caattatttc
     5761 tgaatcacat ggttaggaca tgtgttcacg ctcgtctcaa gaatatgtaa gtagctacca
     5821 actacttata ttaaaagttg atcggatacc ttttccgatt gcctttattc taccaaaaaa
     5881 aggcgagcaa ctcaacagaa gaacgtttat ttagtgtact taaattaaat attcttaaac
     5941 actgaagaga gaacacatac tatggctgag attaaagcta aactgtgttc tctttttgtt
     6001 tgagaaagga cgttacaatc gatgttgagc gaaatttatt cagctattct taaaaacggc
     6061 ttacgtacat acaaatatcg aaacagcacc atgaaacctg caggtcatat tgattccagt
     6121 aggagtggtg ctatctttgc gtaccgtagc aagcaattaa tgaatgttgg gcgtggtatg
     6181 gtcattactt cggaagaagc tattttagag aacgaaaaga aactaacgca ttggacacct
     6241 aatacctatc gctttggtac ttatgcggat gattatcgaa cagtggttaa aggtcattct
     6301 gagaaaaact taagtcaaat caacacattt gtagttgata tcgattcaaa agagaaccat
     6361 caaggcgaaa ttattttagc ctgtttagat caagtgggct acatgccaac gctaatttta
     6421 gaatcagatc atggttatca agtttatttt gtactaaaga caccaggcta tgtcacaaag
     6481 aaaagcaatt tcaaggtgat cgatgtagca aaaaaaattt caaaaacaat tcgtctacag
     6541 ttggctgaaa aattgtccgg agtggatcta ggatgtaacc acttcggaat tgctcgattc
     6601 ccacaagaaa ataatattgt ttttaaagaa atgaactatc agtattcttt tgctgactgg
     6661 attcaatggt cgatgaagct tgaatcagat caaaaaacaa aagaagatcg agaacgaaaa
     6721 ctaattgtct ttcctgagaa aaaagaaatt cgtcaagtag atgaaccgtg gttcgatatg
     6781 ttacttcata aagcaaacat cgtcggcgga gaaggacgtc ttggtcgaaa taatgtgatt
     6841 ttcacgcttt ctttagctta ttactcttca ggttatgcat tggatacttg cgaatacaat
     6901 atgttcacat tcaacgaacg gttacaggaa ccgctatctg aagcagaatt agtaaagatt
     6961 gtacgtagtg cttattctgg taattatcaa gcagcaagca gagaattcat tattgaattg
     7021 tgccagcaat gggtttcacc aaccttaaaa aataatgatc tgttcattca acgacgtggc
     7081 tggtggaaat ttaaaaagcc acgagatcaa agaaaatata gtcataaaaa tgaatggaaa
     7141 gaagatctat tggcttatct aagcgaaaaa agtcttgcta gtgagcctta tttgaacctg
     7201 tcaaaaaaag aattggcttc acaactaacg atgcccaagc gaacacttga taagatctta
     7261 aatgagttac gagaggagaa taaagtcttc tacaaggtaa cacgaggaag aaatggcgga
     7321 atgatcattg cttcagtcaa aacgttgttc gcgagggtta ttcaactaag taaagaaaag
     7381 agactagatt attcaacaag gatcacagag gtatttggac tcaacgagag cttagtagaa
     7441 gagacattta gacaattatc aacccccttg aaccctctta aagaagtgaa tctttttgaa
     7501 gtagatacag gttgaaaaat aaaaggtgca aattgcctta tacaatatat ctatgatact
     7561 tctgtaggtg ttgctccctt tgcctagcta cgtgtaggtg gtatacttat aactgcgtat
     7621 gcagtcttat gtatcatgga ggaagttgta tgtcgttaaa aaattatgtc tattcacatc
     7681 ctgttaatgt gtttatcatt ggtcgtttgg gattatctgt ggaagtgttc tgtgagcttt
     7741 atggttttaa gcaggggact ttgtcttctt gggttaccag ggaaaaaaca gttgcttctt
     7801 tgccgattga gtttcttcat gggttgtcct tagcttctgg ggagactatg gatcaggtat
     7861 atgattgttt gtgtgtgtta gagcaggagt atattgagta taaaatagct aacgagttaa
     7921 gaaaaaggaa gaagtatatc cagtagtagt ttttatttac ttaaaaaatt ggttcattga
     7981 gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt tagggaagaa
     8041 atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa aagaaatagc
     8101 aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg tagttacggg
     8161 gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac ggcttattac
     8221 ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct aatgacgttg
     8281 atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac caggcaaaaa
     8341 ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa tgcaattcta
     8401 gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt gaaggatttt
     8461 ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt gggtattatt
     8521 ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga aaaagaaatt
     8581 gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt tggaagatat
     8641 gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac tgttaagggc
     8701 atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag aaaagagatc
     8761 tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt gtggttagat
     8821 ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga tttagaactt
     8881 ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta tacaggattg
     8941 tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc aaagtgaaag
     9001 aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa taccgaacag
     9061 aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa caagagcaag
     9121 aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat tcaaggaatc
     9181 taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga aacgtgggaa
     9241 cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa aaataaatgt
     9301 gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt aaaaggaggg
     9361 tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt ggaggaatta
     9421 atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt gatcgaagat
     9481 gtgac
//
LOCUS       DBNIBO010000107         9425 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.111, whole genome shotgun sequence.
ACCESSION   DBNIBO010000107 DBNIBO010000000
VERSION     DBNIBO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.111"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(257..1021)
                     /locus_tag="AC1Y77_002075"
     CDS             complement(257..1021)
                     /locus_tag="AC1Y77_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HIQ1575631.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(1132..2001)
                     /gene="mdcB"
                     /locus_tag="AC1Y77_002076"
     CDS             complement(1132..2001)
                     /gene="mdcB"
                     /locus_tag="AC1Y77_002076"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HIQ1575632.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(2026..3102)
                     /locus_tag="AC1Y77_002077"
     CDS             complement(2026..3102)
                     /locus_tag="AC1Y77_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIQ1575633.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(3126..3719)
                     /locus_tag="AC1Y77_002078"
     CDS             complement(3126..3719)
                     /locus_tag="AC1Y77_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HIQ1575634.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(3723..5399)
                     /gene="mdcD"
                     /locus_tag="AC1Y77_002079"
     CDS             complement(3723..5399)
                     /gene="mdcD"
                     /locus_tag="AC1Y77_002079"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HIQ1575635.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(5389..5688)
                     /locus_tag="AC1Y77_002080"
     CDS             complement(5389..5688)
                     /locus_tag="AC1Y77_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HIQ1575636.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(5790..7436)
                     /gene="mdcA"
                     /locus_tag="AC1Y77_002081"
     CDS             complement(5790..7436)
                     /gene="mdcA"
                     /locus_tag="AC1Y77_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HIQ1575637.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(7443..8354)
                     /locus_tag="AC1Y77_002082"
     CDS             complement(7443..8354)
                     /locus_tag="AC1Y77_002082"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIQ1575638.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            8474..9331
                     /locus_tag="AC1Y77_002083"
     CDS             8474..9331
                     /locus_tag="AC1Y77_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIQ1575639.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         2652 a         2048 c         1684 g         3041 t
ORIGIN      
        1 aaactaaaat agtgcttcct atttttcgtt tggttgttct tattatcgca gatgcaagct
       61 tttaaacacc atttagtgga gaggttgtga cagagatgtt cagccttgag cgataagaag
      121 cgattttgaa aaatcgcttc ccatatttta caaaatcgtg ccttattgcc gataggctat
      181 ctctggcaca ccgtttattc agaaaaagga gctgcgacaa ggcttttgtc acaactccgt
      241 tcataaatat ttcctgtcaa tccagcataa tcggctgaat aaaatcggct tgccatcttt
      301 tttctttctc agaaaaatca aaataccata tctgagaaaa atactgtcgt tgtgtcttac
      361 ttcctgagat caatcgccca tcttttcgat cgactgaata atcacagcat ctgaaatcaa
      421 ttcggatggc aaaactattt tcgtggatct gtcgataatt tcctacttcc tgaatagtta
      481 tttttttagt atgatttctt acaccgtctg ctttgttttt ttgcagaact ttctgatgtt
      541 cggcaaacag cttgtctgta taacagcttc tggctttttc aggagtttct tcttcccaag
      601 cggattggat cttcatgaat atcttttgga tttccagaaa ctgtgctttc ttttctttag
      661 ccgttcctga catgcgggac aatatctccg ctttcagcct tttcttttgt cttttattct
      721 tgacgataga aaaagcagaa acccctccta ctgacaaaac agctaatgca agaaatcccg
      781 tttgctcata actagaagga cctgtacctg gaccataata cccataagaa tctctgccgt
      841 aagaacgact gccaaaataa ctgcccccat atgaacgacc accaaatgaa cctctgcccg
      901 aacttcgacc gctagatgta cctccaccat gaccacccgc gacagcgaat ccttgggtcg
      961 gcagagcaaa caaacatagg cccactaaca aaatgattgc taaataattg atttttttca
     1021 ttgtcttcct cgttatcttt tattacaaaa aactattttt atgatagcct tgctaaaagg
     1081 aagagtcaat aaccagatgc ataaactgtt tttttagcaa ttttgtctta tttagtcatt
     1141 gagtaacatc ggacaaatgt atgtgtcggc agtccataat tcctctatct tcagcatgaa
     1201 caagcttgca gacaagagat ctgcgcttcc tccaggagag atttggtttt cctgacaaaa
     1261 gtgcaacagt gctgtaaatg gacgatttgg taaagcactt tgactagcag catgagcaag
     1321 tttttgcatt tccactaacg ttttctgatc actgcgatgc aatacgcatg tgtcatctag
     1381 cgaactcatc aaaactaaca acatatgcat ttgtttctgt acatatgaat cattatctag
     1441 ctccttagct gcttgaattg caagcctgat atgcggatac cctaatatcg cttcttcata
     1501 tgcaccattt acgccatatt tttgcttcac cctatgacca tgagtggttt ttttatgacg
     1561 atattttgta tcaggaaaag cagcaagctt gctgacatca tcaagtattt tcgataaaga
     1621 aaaaggctgt tccatactga ttttttgtgc aatgacactg atcaatagcc ccattgccca
     1681 aatcgctcct ttatgggtat tgatgccatt tgtagcaatc agcattgcct tttccgcttt
     1741 tcgcccaatc tcagcaattc tttctcttaa ctcctgatca atagcatgat gccatgctat
     1801 ttgagccatt tcttcaaaaa aaggagttag cgtcttagca gacttaagaa ataaagcata
     1861 gtccatatcg gaatgtgcac ccgcatccag tgcatccact aatccaggtt ttggacttaa
     1921 tgttacttct gcgatcaatg cttcctctac caacgcagcg agttgctgag tcactgcttt
     1981 ttttcgttcg tcatttatca tgacactcac tccttcttcc tactattact tgctgaaacc
     2041 ttttttgata gctacccaaa tgatatgaag taaccagctt aaagcaaatg cacaacttgc
     2101 cgtcattaga taagctacaa gaatcatgac tcctgaaggt aactgcagcc aactatcttt
     2161 aaacaataat aacagtactt ggaaaacaaa tacattggct aaatacgtac gataagcata
     2221 aacagacaac catttgaaca atggcaattt tggcgaatcg tttacaatca ttttggaagc
     2281 gcctatgaag acagcaaaga tgatgactaa actgtataaa ctttggatcg tattcaagta
     2341 aggtgcattc gcaaagctca agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa
     2401 cgcgataact aaggcaacag gtaagaaaag ataacgtact ctgtccaaat attgatagac
     2461 tttttcgtga taaacaaatg cggctacccc ataaatcatg aaactagcaa caaaccgatc
     2521 taataagtac cagctgtcat gataaggccc agtcaatact tgtgtttgat agaacagata
     2581 ccatgcgaca tataaaatcg ttgaaccaat caatactggc catacatatt ttttattagc
     2641 aaatactttg taaccgagcc aaacaaaaaa cggcatgaac aattggatct gcagcatcat
     2701 aacggtatac cataaatgag gcgctccatc acctgtgaca tatttcagta aaaagcttgc
     2761 agtatccgta tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt
     2821 ccatagaaga tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga
     2881 caaatgctga cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt
     2941 gtatttaacg atgacgtaaa aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt
     3001 tgccaaagca aagctcaata tcgtttgcat cataacagcc gttacagctg atactttctg
     3061 gaagtcactt acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt
     3121 ctttattacc aaagttcttt ggttaattta ggtccttcga ctgttttcaa tagaattttt
     3181 ttctctggct ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg
     3241 gtatcgaatt gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc
     3301 ggtgaatttg cgtacagcag caaatcaaaa tcactggttt cttttactgc cggaatccca
     3361 gtcgataatt caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt
     3421 ctagcagcat gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat
     3481 tcttgttcga tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct
     3541 gttgaccatc tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga
     3601 tccgtcgacg cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc
     3661 aaggtgaacc cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt
     3721 ttttatgcat cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg
     3781 aatgttttgg ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg
     3841 acatcttgaa tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca
     3901 gcttggtcgg tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg
     3961 atatcgtaag cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt
     4021 tttgtcacac gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt
     4081 aaagcaatca agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt
     4141 cctgcaatca atccaataac tggatgtcca gcttgacgga gttttgcata agcagcagca
     4201 ctattagcca aagccacatg gataccgatc aattcttcgt tatagccgta tgcttggctt
     4261 ggtacatcga tcacaacgat gattggccgt ttcacttttt tgtctttatc ttcttcgtaa
     4321 acgtgatcca aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt
     4381 acacggtaca tcgggttttt aggatcaggt acgattgatg tcacgatggc ttcttctatg
     4441 aatttacttg atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca
     4501 gttagtgccg caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct
     4561 acttctggta agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca
     4621 atggccattg gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt
     4681 tcactgcgat gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct
     4741 tctacactat cagtcactaa ttcatcaatg atttttgatt ctacccgttg acgagatcct
     4801 aatgtattcc aaatcaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt
     4861 tcaataactt ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc
     4921 attaatgctg aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg
     4981 atgactggta catattgttt tagagcaatg attacatttc cgatttctga aatagataac
     5041 agtccgtagt ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag
     5101 atctcatttc cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca
     5161 ccggaaactt caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct
     5221 tgtttgccat tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt
     5281 ccttgtgctg gtaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca
     5341 ttgtcaagta gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc
     5401 tgaaagttcc tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa
     5461 gtcattgatc acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt
     5521 gttccatact tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt
     5581 ttctgttgga aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt
     5641 cttattgatc ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc
     5701 tttttgtaaa acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa
     5761 atggcgacaa aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt
     5821 tcatacaggc catctgacca ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa
     5881 cgtttcgctt cactacgttt gatgcctaga tcttctggta atttcacgat gccggctgca
     5941 cgcaagctat ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt
     6001 gctgcaattg ctgcttgtcg ttcttctttt gtttttgctt tgtacaaata agcaattcct
     6061 tcttctgtca cgatatgtgt agtatcttcg ctataaatca tcactggtgc attggctaag
     6121 cctgcttgtt tttgaacagc tagtgcatct aattcttcga caaagactgg ttttttattt
     6181 gaaccatatg tttcaaccat ttgaacaact aatttttggc ctttccctaa tggatcatca
     6241 ttgtcgcgaa gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc
     6301 atgttaggcg caccgccaaa accagataaa cgtccttttg ttacggtaga agagtttcct
     6361 tcgtaatcta attgcaaggt tgaaccgata aacatatcga tggcatattg tcctgcagct
     6421 tgtgataatg ttcggttact gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg
     6481 cgagcagcaa tatatttttc cataccaact tcgccaccaa aactatggat gctttcaacc
     6541 caacctgatt cgatggctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg
     6601 attttccctt ttaaccctag tgattcacca taagttggca gtaacaattc gatagctgct
     6661 gtattgaatc caataccgtg attcaaagat tgtacgccgt gttctgcgta gattcctttg
     6721 attgccatca ttgccattag aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat
     6781 aaagcttcta attgataagg tttatctgct ttcacgacaa cgtctaccca gtcaccaggg
     6841 atatctacac gtggtaaaga atcgacaact tcgttaactt gaacgatcac gataccatct
     6901 ttgaaagctg cagcttcgat gattgttggt gtttcttccg tattgaaacc tgtgtacaag
     6961 ttgccgtctt tatctgcttt atctgcagct actaaaacaa catttggaat caaatcgatg
     7021 aataagcgtc cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct
     7081 tcaatcattt gggcgacacg caagctttgt gcaccagcat aagagaaatc gatcttgctt
     7141 gcgattcctt tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa
     7201 tgcagatcat tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct
     7261 tgtttttgat tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt
     7321 acttctacga ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc
     7381 aatcgctgcg cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt
     7441 agtcatctcc atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg
     7501 atcgtacgga tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg
     7561 ccggggggaa attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg
     7621 ttccactgca cagggaaagc taggttttcc aacaaatctt tacgaatctc ttctgcgtcc
     7681 tgagtggctc ttccatccgt attttttata tacaaacatc tagcatcaga tagatggaca
     7741 gactgcatat acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc
     7801 acagggactt tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta
     7861 acggataaac cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac
     7921 agataaactg gatggtcagg atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc
     7981 gtcacaccta ctactacgcc cattgcataa ccgcttggat aagcttcttt cattaagact
     8041 gcgcggtgat agactagttc cgctgcattc tcaaatctca aagatcgaca agcaacagct
     8101 gcacaaaaag caccaatcga atgtcctgct gcaatatcag gctgaatacc gtttttttcc
     8161 atttcatcta aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct
     8221 tgatagtttt cttctttatc aactaaccga taccccgtca cattttctaa acgttccatg
     8281 tacatttcag ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag
     8341 aaacctgtac tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc
     8401 aatttctgct ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat
     8461 gggatggata attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa
     8521 aaatgttacc caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat
     8581 caaacatttg gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga
     8641 gacgaccgtt tatgcagaag aattttatcc atatgcagtc aagacattag acacattaga
     8701 agaaggaatc aaattattgt cttcggtcaa tgagaagatc gttacttccg tcatattgta
     8761 tttagaggta gcctctgttt ctattccgaa cttggtccga atttttatgg aaaagcatcc
     8821 tgatatccaa ttaacgatca tgcaacatca tttaccagat gatattaccg atcctgtact
     8881 gtacatcact tcggagatga agcctggcat gaccaatatt cctatcatga aagaacctgt
     8941 ttatgtcgcc attcctaaga aacatcctct tgctaaaaaa gaacatctgc aattagaaga
     9001 tatccaacgc acccccatct tgatgctgtc aaagagcaac gccttccgtc atacactgga
     9061 tgcagcactt gaagcgaaaa gtatcgaatt gacgatcagt tcgacgacag atgatcctgc
     9121 gacattacgt tctattgtca agcaaggatt agctgttagc tttttcccaa aagtttcttg
     9181 gtcttacgat aaaaatgatc ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg
     9241 aacaatttat ttatcttcta gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa
     9301 aacgatgcgg gaattttatt taggaaaata aaaaaaaaag agctgggaca aaattcttgt
     9361 cgcagctctt ttttcgaaca aacggtgttc cagaagtaat ttcttagaaa ataagccgaa
     9421 atttc
//
LOCUS       DBNIBO010000108         9353 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.112, whole genome shotgun sequence.
ACCESSION   DBNIBO010000108 DBNIBO010000000
VERSION     DBNIBO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.112"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            2..1277
                     /locus_tag="AC1Y77_002084"
                     /pseudo
     CDS             2..1277
                     /locus_tag="AC1Y77_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744739.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1364..2395)
                     /locus_tag="AC1Y77_002085"
     CDS             complement(1364..2395)
                     /locus_tag="AC1Y77_002085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575640.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(2402..3232)
                     /locus_tag="AC1Y77_002086"
     CDS             complement(2402..3232)
                     /locus_tag="AC1Y77_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575641.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(3229..4044)
                     /locus_tag="AC1Y77_002087"
     CDS             complement(3229..4044)
                     /locus_tag="AC1Y77_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575642.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(4041..4865)
                     /locus_tag="AC1Y77_002088"
     CDS             complement(4041..4865)
                     /locus_tag="AC1Y77_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIQ1575643.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4855..5955)
                     /locus_tag="AC1Y77_002089"
     CDS             complement(4855..5955)
                     /locus_tag="AC1Y77_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1575644.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            6133..6918
                     /locus_tag="AC1Y77_002090"
     CDS             6133..6918
                     /locus_tag="AC1Y77_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIQ1575645.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6951..7334
                     /locus_tag="AC1Y77_002091"
     CDS             6951..7334
                     /locus_tag="AC1Y77_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIQ1575646.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            7410..7541
                     /locus_tag="AC1Y77_002092"
     CDS             7410..7541
                     /locus_tag="AC1Y77_002092"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1575647.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            7510..7746
                     /locus_tag="AC1Y77_002093"
     CDS             7510..7746
                     /locus_tag="AC1Y77_002093"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1575648.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            7758..7844
                     /locus_tag="AC1Y77_002094"
                     /pseudo
     CDS             7758..7844
                     /locus_tag="AC1Y77_002094"
                     /inference="COORDINATES: protein motif:HMM:NF012387.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(7824..8795)
                     /locus_tag="AC1Y77_002095"
     CDS             complement(7824..8795)
                     /locus_tag="AC1Y77_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIQ1575649.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2767 a         2155 c         1804 g         2627 t
ORIGIN      
        1 tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg tcgagaagaa
       61 aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc
      121 ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa
      181 atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt tggcaaagca
      241 acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca ttgttgaaaa
      301 aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga ctgaaaaaca
      361 agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact
      421 cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct gttcattggt
      481 agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt gtatggatgg
      541 cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc tcatttctta
      601 ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa
      661 cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg ttgtggatcg
      721 ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat gagtcatgaa
      781 aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga aggctttatc
      841 tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa aatggcgtaa
      901 ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt ctacttctga
      961 tgaactacga atagcttatg cttactatca agaaattctt caagcttttt atgacaaaga
     1021 agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa gagaactgca
     1081 tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct tagagttgcc
     1141 ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga aacgaatagc
     1201 ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac ttattaaaca
     1261 taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata tcactctatc
     1321 agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt tgttgtgcaa
     1381 aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga atgtataagg
     1441 catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct ttgccctcca
     1501 gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc catagaaccg
     1561 gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt aaataataaa
     1621 tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca taccattcga
     1681 aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc tgatacaatt
     1741 ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc cgatcactga
     1801 ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct agcgattgct
     1861 ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg
     1921 gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt gccacccgtt
     1981 gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc atatccacca
     2041 atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc tttaccttca
     2101 aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac tgttgaaaaa
     2161 caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg tccagcacaa
     2221 tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta gactttccgc
     2281 acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt tcatcaatag
     2341 ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa aacattttcc
     2401 gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc tgcttaaggg
     2461 acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt cccaccataa
     2521 acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc atccgccgca
     2581 gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc agcatgtact
     2641 ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata cggatgtttg
     2701 gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct gctgcatcca
     2761 ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag acaatcggca
     2821 acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca gtttcagcat
     2881 acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc agcaggagca
     2941 gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata atggtgggaa
     3001 tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag cgacccaccg
     3061 cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta aaatcactag
     3121 gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca ataatcggca
     3181 gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc acgtctgcac
     3241 caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac ctaaagcgac
     3301 tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta aagccgcaat
     3361 tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt cataggtccc
     3421 catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa
     3481 agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg actccagcca
     3541 ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg gaatttggaa
     3601 ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     3661 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     3721 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     3781 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     3841 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
     3901 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
     3961 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
     4021 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
     4081 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
     4141 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
     4201 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
     4261 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
     4321 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
     4381 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
     4441 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
     4501 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
     4561 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
     4621 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
     4681 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
     4741 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
     4801 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
     4861 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
     4921 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
     4981 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
     5041 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
     5101 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
     5161 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
     5221 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
     5281 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
     5341 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
     5401 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
     5461 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
     5521 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
     5581 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
     5641 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
     5701 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
     5761 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
     5821 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agccgcagcc
     5881 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
     5941 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
     6001 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
     6061 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
     6121 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
     6181 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
     6241 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
     6301 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
     6361 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
     6421 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
     6481 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
     6541 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
     6601 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
     6661 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
     6721 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
     6781 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
     6841 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
     6901 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
     6961 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
     7021 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
     7081 attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt gcagccttta
     7141 aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa gtagccgccc
     7201 cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg
     7261 cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga
     7321 ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct ctctttcaat
     7381 tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc
     7441 tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc
     7501 aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt tattcaaggt
     7561 ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt tttcgctgat
     7621 gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga ccacggcccc
     7681 cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc tgacctgcta
     7741 atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt cgttatactg
     7801 tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct cagccgacaa
     7861 acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc gaaaaaacat
     7921 cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc tgcagctagt
     7981 gagtgtcttt catttatcca taagataaag cttggattca ataaataaat aaatgttttg
     8041 gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc caatgcaaac
     8101 atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc caatgcttgg
     8161 taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa ccgatcaaga
     8221 tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat acctggaacc
     8281 acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc
     8341 ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct ggtaaacatt
     8401 tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc attataagca
     8461 ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt ttgcaaaatt
     8521 tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac tccgccatcc
     8581 gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc agcggtcatg
     8641 gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga acaccaggca
     8701 cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc atcatgtaga
     8761 gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt acacactagt
     8821 ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc tcatataaat
     8881 atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact aatacaggta
     8941 gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat tcccttattc
     9001 gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac
     9061 atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa aacggagtca
     9121 gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg taaagtacca
     9181 cagggctgta tattttttat ttcagattac cttaaatgat tccatttact ttttatctca
     9241 ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact atttttggat
     9301 acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg tta
//
LOCUS       DBNIBO010000109         9264 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.113, whole genome shotgun sequence.
ACCESSION   DBNIBO010000109 DBNIBO010000000
VERSION     DBNIBO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.113"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            1..226
                     /locus_tag="AC1Y77_002096"
                     /pseudo
     CDS             1..226
                     /locus_tag="AC1Y77_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            352..1323
                     /locus_tag="AC1Y77_002097"
     CDS             352..1323
                     /locus_tag="AC1Y77_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1575650.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1463..2776
                     /locus_tag="AC1Y77_002098"
     CDS             1463..2776
                     /locus_tag="AC1Y77_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1575651.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            2819..3685
                     /locus_tag="AC1Y77_002099"
     CDS             2819..3685
                     /locus_tag="AC1Y77_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575652.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3682..4530
                     /locus_tag="AC1Y77_002100"
     CDS             3682..4530
                     /locus_tag="AC1Y77_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575653.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4544..6016
                     /locus_tag="AC1Y77_002101"
     CDS             4544..6016
                     /locus_tag="AC1Y77_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIQ1575654.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6033..6905
                     /gene="scrK"
                     /locus_tag="AC1Y77_002102"
     CDS             6033..6905
                     /gene="scrK"
                     /locus_tag="AC1Y77_002102"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HIQ1575655.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7141..8523)
                     /locus_tag="AC1Y77_002103"
                     /pseudo
     CDS             complement(7141..8523)
                     /locus_tag="AC1Y77_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8985..>9264
                     /locus_tag="AC1Y77_002104"
     CDS             8985..>9264
                     /locus_tag="AC1Y77_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HIQ1575656.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         2826 a         1601 c         1933 g         2904 t
ORIGIN      
        1 tcttgaaaaa ttaattaaag acttagataa agccattgac gacttagtcg tggtgctacc
       61 ggagtatcaa tgtttaacca gtatcccagg tattggtcct gtttaggctg ctggaatact
      121 cgctgaaatt ggtccaattg aacgtttttc tgatgaaata aaattgacaa agtatgccgg
      181 cctatattgg cgacagagtc aatccggtaa atctgaatac gaaaattttt ttacgaaaag
      241 cttcacgtct aatatctatt ttaaaataaa aatattcctg ctaatttgga atcgtttcct
      301 tcttttggta taataatacc tgaaattctt ttgagaggag ttgttcgtga agtgaaaact
      361 actatgaagg atgttgctgc gttagcagga gttggtgtgg gtacggtatc tagagtaata
      421 aatggagtaa aggtgaaaga ggttaccaaa gaaaaagtaa tggctgcaat tagtgagctt
      481 aattatgagc ctgatgaata tgccagaggt atgaaaacga atagtagcaa tacaatagct
      541 cttattttac ctacgatttg gcatcccttc ttttccgagt ttgcctacta tgtagaggaa
      601 gctctcagcc aaaaaaatta taagcttttt ttgtgtaatg cgaacggcag tgcggaaaaa
      661 gaaaaagaat atatccaaat ggtcaagcag agtaaagtag acggaattat aggtattacc
      721 tattcagaca ttgataaata tgtatcatca aatttaccat ttgtcagtat agaccgccat
      781 ttttcggaac aggtttctta tgtaacggca gataattatt taggtgggca aattgccgct
      841 caagaactac ttactcgtaa ctgtaaaaac ttgtgctatg ttgggggtgt ttcaccttac
      901 cctaacgaaa caaacaatcg taaagctggt ttttacaata tttgtgagga aaataaaata
      961 gctgtgaagg tattggatat gcctgaacca attagcaatt tagaggaaca attgctcgct
     1021 tttttttcgg ataatcctga aattgatagc atttttacca tcaatgattt catggctttg
     1081 gatgtgataa aagcccaaaa aaaactaggg aaggaagcac ctaaggattt tcaaattatt
     1141 ggttttgatg gagtcaagct atcgttgcaa aaagactatt tactatcaac gattgtacag
     1201 ccgatagagg aaatggccaa ggtttcagta gaaacactca tggcgatgat tttaaatgaa
     1261 gaaccgccta agcgtgtggt agtaccggtt ttctttcacg ctggagagac gactaaagag
     1321 taaaaaaccg gtttataacg atttttttga gataggttga cagaaagtga tttcaatgat
     1381 atcttaaaac caattaattg gaaacgttac caattaattg aaaaataaaa cggaaacgtt
     1441 tcaaaaaata ggaggaacat cgatgaaaaa gagaatgtta gcaattgcag caattttagc
     1501 attaacagct ggaggacttg caggatgtgg cagtaaggag aaaagttcag gtagcagtga
     1561 tccaggagaa attaagattt gggttcagtt ttctgatgaa acgcctgaag gaaaagcttg
     1621 ggcagagatt gtgaacaatt ttaatgatga atacgagggt gaatataaag ctgttactga
     1681 gtatattcct cgtagcggaa gcggcggtgg atatgaggat aaagtaaatg ctgcaattac
     1741 aaccaatacc cttcctgatg ttattacgtt agatggaccg aatactgcag cttatgctca
     1801 ttcaggagtc attgctccat tagatgatta tttgaaagat gcggacatgg atgatgtatt
     1861 agacagcatt attcagcaag gaacctacga tggcaaaatg tatgcgtttg gtttctcaga
     1921 gtccagtgtg ggtgtctatt acaataagtc gctgttcaaa gatgctggaa ttacagaaga
     1981 tcaattgccg acgttagaag atccttggac ttggacggaa tttcaagcaa tttgccaaaa
     2041 gctgactgat aaatacaaaa cgcctgcaat tgatatgcaa atggcgtcgg gagacgaaat
     2101 gctgacctac gcatatacac catttatatg gtcgaatggc ggcgatgtcg ttaatgctga
     2161 tggaaccaaa gccgaagact attttaacgg taaaaaatcc gttgaagcgt tggaatttat
     2221 tcaagaattg gttgaaaaag gttacaccac aatcagtcct attgaaaaag gatttgaaac
     2281 tggcaaatat gccatgatgc tgagcggttc ttggacaatg gcagatttag aggaaaacta
     2341 tcaagagatt gattatggta ttttgcctta ccccgtttca gatgcaactg gggagttagt
     2401 ttcaccaacc ggaagctggc agttagcaat gactgagaaa acagagaaaa aggatgcttc
     2461 tgcggccttt attaagtttg caaccagtac agaatctagt cgtattatga gtttagggaa
     2521 cagtgttcta cctatcagaa attcaacagt tgatttaatt aaagatgaag tgtcggaagg
     2581 aatgcaggta ttaattaagc aaaaccaact ttctggacat gcccgcccag tggttgtagc
     2641 ttatccgcaa gtatctagag cattccaaca agcagttcaa gatattagct acttcgagga
     2701 aaacccagat gtacaaaaag tagcagatag tcgcgctgct gaaatgcaac aggcaattga
     2761 tacttcacta aaatagaagt ttctaaaggg ggccaatcaa gtgttaaaaa aaccgacaat
     2821 gaaagacaat ttgataggtt acggctttct agcgccagct ttgctgctgc tgacgatttt
     2881 tctgatcata cccgtaatca tggtaattta ttatgccttt actgattatt atttgttaac
     2941 gccagatgct agacaattta tcggcttaga aaattttaag cgactgttcg ctgatccaat
     3001 ttttgtaaaa agtatttgga acacggctaa atttgttgtt tggattattc cggtccaaat
     3061 aggggcagct ttaggaatgg cacttattat caacaaaaat cgtaaaggaa atatcttttt
     3121 taaagtagcc ttttttgcgc cagtcgtgat gtccttagtc gttatttcga ttttatggtt
     3181 gtacttgtta aatcctaatg aaggtctggt taatacgcta tttgcaaaga ttggaattgc
     3241 ttcgcagcct tttttgacta gtcccaaaca agcaatgtat acgcttgttt tcgtatcggc
     3301 atggcaaggt gcaggatatc agatgctgct gtttttaggg ggaatgcaaa atattccgca
     3361 agacgtttat gaggcagccg aaattgatgg tttttctaag tttcagcaat ttcgctatat
     3421 caccatgcca ttattaaagc ccacggcgat ttttatttta cttaccacat tgatttcggc
     3481 ctttaaacta atcattcagc caatggtaat gactcaaggt ggaccaatga attctactat
     3541 gacaatggtt tattacattt accaaactgg atttacagac cgtatggtag gctactccag
     3601 ctccattgcg ttaatcttta ccactatgat tggtttaatt acaattgctc aaagaaaatt
     3661 ggtaaaggag gatgatccag catgaccaag aaatttggac cgctaatgat cgtagaatat
     3721 tgtttgctgt tccttttagc ggcattattc attttcccga tgctgtggat ggtagtttcc
     3781 tctatgaagc ctgaagcaga tgtctatcaa aatttagctt ccttcaaagc ttttttacct
     3841 agtttgaatc cagctaattg gtttaaaact tatcaagagg ttatttcccg gttcagtgtg
     3901 ggaacttatt tattaaacag tattttttat ggtttaacct ttgcctgtgg gtcaatcatt
     3961 gtaaactctt tggcgggttt tgcctttgcg aaggttaatt tttcaggcaa gaaaattatc
     4021 tttggagctt tgttagctct gctgattgtc ccgatggaga cggttttaat tccgcaattt
     4081 accatcgtta attcactagg cttagtgaat actcgcttgg cagtgatttt accagcgatg
     4141 gccagcgcgt ttaatatcta tctgtttaga aatttcttta tcgcgattcc agaagaaatc
     4201 attgaatcag cgaaattaga tggtgcaaat attgttaaaa ttttctttca catcatgctg
     4261 ccaatgtcta aaccagccat agccacagta ggtgtcttat cctttatctc cagctggaat
     4321 gattatattt ggccgttaat ggtcttaact gacagcagca aattttcaat gcaagtggca
     4381 attactacga tcaatactac acagccagtt tatatcaatc aagtgatggc tgttttaacc
     4441 attgcaacga ttccactgat tattgtttat atcgttgcac aaaaatatat tctgcaggga
     4501 cttggcggtt ctggaacggg tataaaatag gagtgaatag tgaatgttgc aaaaactttt
     4561 aaaggccaat gagtttattg agaaaaatgc tcaaacagtc aatcaagaat accggaataa
     4621 atttcatttg atggctccga ttggctggat gaatgatcca aatggttttg tttattatca
     4681 agaggagtat cacttgtttt atcaatatta tccttacgac agcatctggg gaccaatgca
     4741 ttggggccat gctaaaagca aggatctaat tcactgggag catttgccag tagcactggc
     4801 accaggtgaa agctatgaca aggaaggctg tttttccggc agtgccatag aaaaggatgg
     4861 caagctgtat ttgatttata caggacacaa tgttgtggaa gggcaagttc gacaagttca
     4921 gtgtttggca gtttcagaag atggcattca ctttgaaaaa tatgccggaa atccaatcat
     4981 cgcagaagat cacttagctt ctgttgcaac gaccgaagat ttccgcgatc ccaaagtgtt
     5041 tcagcgaaaa gaagactatt ttactgtagt cgctactaaa actgcagatg ctcgtggaca
     5101 aattgtgatg ttccattcca aagatttact aacttgggag ttttattccg tattgctgga
     5161 gggaactcca gaacaaggca ttgtatggga atgcccagac ttgtttgaac tagatggcaa
     5221 agaagtcttg attctttctc ctattcaaat gactccacaa ggcaatgctt accaaaatac
     5281 gaactcaact gttgctttca tcggtactgt cgattggcaa acaggcaaat ttctagtaga
     5341 gaattttcat gaaatcgatg gcggtttaga tttttatgca ccccagacct gtgaaaacga
     5401 agcaggccaa agaataatga ttgcttggat gcaaatgtgg cagcgtaaaa tacctacgaa
     5461 tgatctgggc catggttggt caggttccat gaccttgcct agagagctga gggtggctga
     5521 taatcaattg ttgcaacggc cagtttcatc aatttatcaa gtaattgatc cgcaggaaag
     5581 gattgaatgg gcgaagactg aagagaatcg cttcttactg aaggacttag tatatgacca
     5641 gcactattta cgcttggaaa tcaatttaaa acaggcaacg aaactagaac ttcactatgc
     5701 gaagggaccc gaccatacat tactgcttaa ctatgatgtt gaacgggagt gctttactgc
     5761 ttccagggaa cacatgccgt atgatttatc aggagctgaa aaggaagtgt tgaaaaagcg
     5821 cagtgtgacg attcctttgg aaaataattg tctaaccatc gaaattttcc gtgatacttc
     5881 agctattgag ctttttctaa atgatcgtct gactatgagt acaacttttt atgagacaga
     5941 aaaaggacag gattttcttt tgctggcaga cagtgaagta accttttcat tggaaagcgg
     6001 cttaattaat ctatagaagg gagagctcag tgatgattta tggttcaatc gaagcaggcg
     6061 gcacaaaatt tgtgtgtgcc attagcgatg aagaaatgac gatcaaggaa agagtgagct
     6121 ttccgacaac gacacctgag gaaacaatac ctttagtcat tgacttcttc aaacaatacc
     6181 aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc catcgcgatt
     6241 ccgccactta tgggtacatt acctcgaccc cgaaagtggc atggcaaaat tttgatttca
     6301 tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat gtcaatgcgg
     6361 ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc gtttactata
     6421 cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc gaagggttta
     6481 gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac tttgctggca
     6541 gctgtccatt tcatggcaac tgtttagaag ggatggccgc tggacccgcc atcgaaaaac
     6601 gcttagggaa aaaaggacaa gaggttgcag aagatgatcc ttattgggaa atcgaagcct
     6661 cctatattgc tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat gtgatcattt
     6721 ttggcggtgg tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac gcatttgcaa
     6781 aattggtcaa tgggtatgtc gaaacacctg agttaagcaa atatatcgtg accccagcat
     6841 tagaagacaa cgctggaacg ctcggttgtt tggcattggc tcgagaagcg gtcttacatt
     6901 cataaacgaa aaaaggctct tcgacattta gtgttggtag agaaagatac aactgaaaat
     6961 ttagtcataa ccagttaggc agagtgctta actggttttt gttttacttg gaagatcaag
     7021 ccttgaatga gataagttct tgctcgaaag ttatggaaat ttcgatagcc gtaggctact
     7081 tgtattgtgc aatagttttt tacaaaaaaa agggggtgcg gatatttttt ttggaccttt
     7141 ttaacaaata tatctttgct tcttccgata ttgaagaggg cttagccttc ctaaagataa
     7201 cttaattcgt tgattattat accaatgaat ataatcatcc aatttgttta taaactgatt
     7261 gattgttgta ttcttccagt cacgttgata aaacatctcg ttttttaatc gtccaaagaa
     7321 accttcgcac gctgaattat ctggtgaata actcttttta gacatagagt gaagcaagtt
     7381 ggctttgttc caatagtcca actaacaata gcaccatcgt aacaatcgac aagtggtgat
     7441 aagtagattt tccctgcgag aattctaaat tcagtaatat ctgttaacca tttttcattt
     7501 ggcttctttg ctttaaaatt ccgatttaat aaattggggt gtgcaggaga aatttcccca
     7561 gcatacgaag aatacttttt tcgtctagaa aatttaacaa atagattttc ctcagtcatc
     7621 aatctacgta caactttttc agaaagcttt ttacccatat tttttaaagc ttggtgaatt
     7681 ctacggtaac catacgcaca gaagttatga ttgaaaatcg tgataattaa tttgacgttc
     7741 tttgtaatac ttatctggtt tctcaagtgc ttgtttttga taaaaatagc tgcttttagg
     7801 aaactggagc tgttgaagaa gctctttcaa ggtgaactga tttcgtaagg catcaatcaa
     7861 tatcgttttt tcttgatttg atagacaaag aagattgacg cctttttctt tttttagcag
     7921 ttcagccgct ccttcgagta atgctttttc gagctgtaat tggtgtactt gtttttgtaa
     7981 gtcttctact tcctttaaaa gtagctcttt ttgtttttcc tgatttttta aagtcataca
     8041 caatggaaaa tcctttccaa gaaccgtgtt tttccattga tatagagtaa ctctactaat
     8101 tcctccagat tcggctaaat ttttcctatt agtttgcggt ttatagaatt ttttcaacac
     8161 agcttctttt tgatcttgtg ttaactttac cgcacttttt cggagttttc tagctcctgg
     8221 tgcaagttct tcacaccgct gccgcaagga ctctctatta ggataaccaa gcatgcgaat
     8281 ggttcgtgca taacattgac catattcaaa ataatgattc accgctattc gtttttgttc
     8341 ttcagaatac tttgattttt ttcggtgctc ctgatggagc tctccggatt ccaaatactc
     8401 tttataccat ttccgtagtg caccaatcga cggataccct aattctcgaa tggttgcagc
     8461 ataagagcaa tcgtatttta aaaataactg aattgccctt acacgctctt tgtgggaaaa
     8521 cacacggaca ttcctcctaa gtttattttt tcgtccagga ttatgtccgc acccccttgc
     8581 tttctgatga cttattctgg ttctttgagt gtcaatgcat agatgtgatg tagctagtta
     8641 gaaaaaaatt ttgaatactc tttttttctt tcagactcct ttttaagtaa gattttgaag
     8701 tgtgagttac ggggtcagaa acttaaaagt ttttgatcct ttattttcaa gtctgatttg
     8761 gcagcttttc ggcagctcat ttatttatgg tcagaaatag atgctgaaat tttaatctat
     8821 tagcaaatag tccagaacgt atgaagagga gtcggatatc atttgtcatt aacaccataa
     8881 atatttataa aattcgtgtt cacctacaat taaatttaat atataatatt cgttgtaagg
     8941 gcttataatt ttttgttaat gaacaagata ggaagaaaca tttaatggac agttttcaga
     9001 gattgtcact agtaaatcaa tttgagttat tggctaaatt tgaggaccaa aacagatctt
     9061 attatgaaag aaaaatcgaa atattgagag aaggttacga gtatcattat gatgatgaaa
     9121 tttggagtga tctttctgag ccatttccga aagaagattc tcgatttgta ttagatgtat
     9181 tgaacatgta tcgagatatc aattttagta aatcaaagct aaacttagat gaaaaccaaa
     9241 ttactaattc atactatact catt
//
LOCUS       DBNIBO010000110         9187 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.114, whole genome shotgun sequence.
ACCESSION   DBNIBO010000110 DBNIBO010000000
VERSION     DBNIBO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.114"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            62..919
                     /locus_tag="AC1Y77_002105"
     CDS             62..919
                     /locus_tag="AC1Y77_002105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIQ1575657.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(990..1847)
                     /locus_tag="AC1Y77_002106"
     CDS             complement(990..1847)
                     /locus_tag="AC1Y77_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIQ1575658.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(1832..4534)
                     /locus_tag="AC1Y77_002107"
     CDS             complement(1832..4534)
                     /locus_tag="AC1Y77_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIQ1575659.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(4547..5836)
                     /locus_tag="AC1Y77_002108"
     CDS             complement(4547..5836)
                     /locus_tag="AC1Y77_002108"
                     /EC_number="3.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HIQ1575660.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            5986..7032
                     /locus_tag="AC1Y77_002109"
     CDS             5986..7032
                     /locus_tag="AC1Y77_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1575661.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            7034..9187
                     /locus_tag="AC1Y77_002110"
     CDS             7034..9187
                     /locus_tag="AC1Y77_002110"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HIQ1575662.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2630 a         1904 c         1672 g         2981 t
ORIGIN      
        1 tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact
       61 tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct
      121 tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt
      181 ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac
      241 attgttattc gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca
      301 catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa
      361 gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct
      421 cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc
      481 cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa
      541 aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat
      601 caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa
      661 tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt
      721 ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg
      781 acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca
      841 tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt
      901 acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag
      961 actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc
     1021 aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact
     1081 tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg
     1141 agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac
     1201 gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct
     1261 tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat
     1321 ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt
     1381 ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc
     1441 acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt
     1501 gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca
     1561 ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg
     1621 ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat
     1681 gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat
     1741 gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa
     1801 accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct
     1861 cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg
     1921 gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt
     1981 ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt
     2041 tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca
     2101 atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat
     2161 gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat
     2221 gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca
     2281 cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac
     2341 caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat
     2401 gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt
     2461 caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta
     2521 agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt
     2581 ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt
     2641 ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa
     2701 cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta tctactaata
     2761 cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt
     2821 taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact
     2881 ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta
     2941 gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt
     3001 tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg
     3061 gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat
     3121 cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg
     3181 tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta
     3241 ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat
     3301 ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat
     3361 cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg
     3421 ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac
     3481 tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga tcatggggat
     3541 agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc
     3601 gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag
     3661 tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg
     3721 gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat
     3781 ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat
     3841 gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta
     3901 atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc
     3961 tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca
     4021 tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg
     4081 gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg
     4141 tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt
     4201 tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa
     4261 aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt
     4321 ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc
     4381 catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta
     4441 tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt
     4501 ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac ttttctactc
     4561 taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt
     4621 ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg
     4681 cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca
     4741 ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa
     4801 ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc
     4861 ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca
     4921 gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg
     4981 cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga
     5041 tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa
     5101 aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt accagatatc
     5161 cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg
     5221 tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa
     5281 atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga
     5341 taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat
     5401 aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat
     5461 cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat
     5521 tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt
     5581 cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt
     5641 ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct
     5701 ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg
     5761 catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta
     5821 tatctttata agccactaat aaaaccttct ttctatttga tatatcttta aagattatac
     5881 ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc
     5941 attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg
     6001 taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac
     6061 agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc
     6121 aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc
     6181 agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg
     6241 attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg
     6301 aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa
     6361 aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat
     6421 acagaacaac atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata
     6481 ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc
     6541 ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt
     6601 ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg
     6661 gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa
     6721 ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc
     6781 gctatccaga ccatgaatct cttgagacag catggttatc atatccctga agatttttca
     6841 attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc
     6901 gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag
     6961 acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca
     7021 aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc
     7081 aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt
     7141 tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt
     7201 tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct
     7261 gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta
     7321 ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg
     7381 catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa
     7441 aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc
     7501 ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt
     7561 ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa
     7621 aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg
     7681 gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag
     7741 caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca
     7801 gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt
     7861 tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac
     7921 gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta
     7981 taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga
     8041 aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga
     8101 acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga
     8161 tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca
     8221 aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt
     8281 tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg
     8341 tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca
     8401 atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa
     8461 agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg
     8521 aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata
     8581 tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt
     8641 tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt
     8701 tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat
     8761 aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga
     8821 ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt
     8881 caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc
     8941 attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac
     9001 gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac
     9061 gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg
     9121 gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta
     9181 taattaa
//
LOCUS       DBNIBO010000111         9115 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.115, whole genome shotgun sequence.
ACCESSION   DBNIBO010000111 DBNIBO010000000
VERSION     DBNIBO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.115"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(777..2408)
                     /locus_tag="AC1Y77_002111"
     CDS             complement(777..2408)
                     /locus_tag="AC1Y77_002111"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 53 protein"
                     /protein_id="HIQ1575663.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="AC1Y77_002112"
     CDS             complement(2422..4095)
                     /locus_tag="AC1Y77_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HIQ1575664.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="AC1Y77_002113"
     CDS             complement(4108..4425)
                     /locus_tag="AC1Y77_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIQ1575665.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="AC1Y77_002114"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="AC1Y77_002114"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HIQ1575666.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="AC1Y77_002115"
     CDS             6087..6872
                     /locus_tag="AC1Y77_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIQ1575667.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="AC1Y77_002116"
     CDS             6875..7567
                     /locus_tag="AC1Y77_002116"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575668.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="AC1Y77_002117"
     CDS             complement(7841..8212)
                     /locus_tag="AC1Y77_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HIQ1575669.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8612..8842)
                     /locus_tag="AC1Y77_002118"
     CDS             complement(8612..8842)
                     /locus_tag="AC1Y77_002118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HIQ1575670.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8936..>9115)
                     /locus_tag="AC1Y77_002119"
     CDS             complement(8936..>9115)
                     /locus_tag="AC1Y77_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575671.1"
                     /translation="VMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILT
                     DYSKIATDKKYILWM"
BASE COUNT         2821 a         1968 c         1513 g         2813 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta attaagaaac aaactttaga aactctagaa gaacttctga atttatatag
     8461 aagtgtgtag ctacttacta aatatacaaa agttaaaata aaatactctg gatactcttt
     8521 atactgcatt aaagttagta atattaacag caaagatatc gaaaaaacaa taacataata
     8581 tgtgtcaaaa tttttcataa taagaactcc cttactgttg catactaaca taatccaaaa
     8641 actcttctac ttcagctaaa gacattccac tttctttagt ccaaaaaaca gttttttcta
     8701 tcgatccttc tctgtaaact gtaaatgtcg gagtcgagtt aattgagtag cgactcttga
     8761 attctttcca ctcattttca tcttctctaa cttttgctac attcacgtaa taaatatttt
     8821 cttttccctt ttctaccagc atatcgaata gcttcctgct tttgatgatc gatttcttgt
     8881 gctacatcta gtaaattttc atcaatttct atatcataaa gtatttcact agtgtctaca
     8941 tccacaatat atacttttta tcagtagcaa tttttgagta atccgttaaa atgatctcag
     9001 tctcgtcact ttcatcagta taaatatacg ttaacaatga actcaccttt tcaatcggtg
     9061 ttagctctaa gcctactgta ttttttacta ttgagtcatt atttaccacc ataac
//
LOCUS       DBNIBO010000112         8958 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.116, whole genome shotgun sequence.
ACCESSION   DBNIBO010000112 DBNIBO010000000
VERSION     DBNIBO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.116"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(1..459)
                     /locus_tag="AC1Y77_002120"
                     /pseudo
     CDS             complement(1..459)
                     /locus_tag="AC1Y77_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(711..1070)
                     /gene="rplT"
                     /locus_tag="AC1Y77_002121"
     CDS             complement(711..1070)
                     /gene="rplT"
                     /locus_tag="AC1Y77_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIQ1575672.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1123..1323)
                     /gene="rpmI"
                     /locus_tag="AC1Y77_002122"
     CDS             complement(1123..1323)
                     /gene="rpmI"
                     /locus_tag="AC1Y77_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIQ1575673.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1395..1919)
                     /gene="infC"
                     /locus_tag="AC1Y77_002123"
     CDS             complement(1395..1919)
                     /gene="infC"
                     /locus_tag="AC1Y77_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIQ1575674.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1963..2088)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2239..2871
                     /locus_tag="AC1Y77_002124"
     CDS             2239..2871
                     /locus_tag="AC1Y77_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIQ1575675.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2873..3778)
                     /locus_tag="AC1Y77_002125"
     CDS             complement(2873..3778)
                     /locus_tag="AC1Y77_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIQ1575676.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3863..4522)
                     /locus_tag="AC1Y77_002126"
     CDS             complement(3863..4522)
                     /locus_tag="AC1Y77_002126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIQ1575677.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4838..5782
                     /gene="mvk"
                     /locus_tag="AC1Y77_002127"
     CDS             4838..5782
                     /gene="mvk"
                     /locus_tag="AC1Y77_002127"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIQ1575678.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5775..6752
                     /gene="mvaD"
                     /locus_tag="AC1Y77_002128"
     CDS             5775..6752
                     /gene="mvaD"
                     /locus_tag="AC1Y77_002128"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIQ1575679.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6765..7850
                     /locus_tag="AC1Y77_002129"
     CDS             6765..7850
                     /locus_tag="AC1Y77_002129"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HIQ1575680.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7847..8911
                     /gene="fni"
                     /locus_tag="AC1Y77_002130"
     CDS             7847..8911
                     /gene="fni"
                     /locus_tag="AC1Y77_002130"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIQ1575681.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
BASE COUNT         2693 a         1859 c         1736 g         2670 t
ORIGIN      
        1 atacctaatg acatcaagat cggcaacaca ataactgcaa tagaaatcac tggtccgatt
       61 cctgtcatcg acatgaatat aacagcggtt acaatacata gtaagctcat cgtgattctt
      121 ggcttgtccc ctccaagttc aactaccttt cgaatcaggg tagctgcgat tccgctatct
      181 actagaacac gcccgaaaaa ggcaccaaag aagacattta ccaagatcga tttggcgtac
      241 tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa ttactccttg cccttccatc
      301 gccgagtttg gtgaaaacgt gtttccgact agcgctaatc ccatccatac agtagccatg
      361 atgacaaaag ccgaccatca agttgtagcc ttttacacag taccagacca tgaaaaagaa
      421 tgagataact aacaaaatac caataataat atcactcatg ttattcctcc gtccattttg
      481 tttttctgat aagcaaacta atatatcagt caaactcatt ctaacttatg tagttagcgc
      541 tttcattaaa ggatgaccat aaatgttcag ttttcaatta aaaaacctta attatgttat
      601 tttagactta tacgttattg tcctgtttca tttgatggag gattgtaaat aaaaaaagag
      661 gaaagaacat aacttttcgt tatcttcttt cctctttata attgtttagc ttatttagcc
      721 aaagcttctt ttgcttggtc agccaatgca gtaaatgctg ctgcatcgtt aacagccaag
      781 tcagctaaca ttttacggtt gatgtcgatt tcagcaactt tcaaaccgtg cattaatttt
      841 gagtagctca agccattcat acgagctgct gcgttgatac gagcaatcca caatttacgg
      901 aagtcgcgtt tcttttgacg acgatcgcga tatgcgtagt tgtatgaatt cattacttgt
      961 tcttttgctg ttttaaataa agtgtgtttt gaaccgtagt aaccttttgc taattttagc
     1021 actcttttac gacgtttacg agttactgtt ccacctttaa cacgtgccat gtataattcc
     1081 tcctaataaa aataaatctt cagattctgt tatttgtact tcttatttca tttttgctag
     1141 ttgttggcga atacgtttga agtcgccact agaaaccatg cttgctttac gcaattgacg
     1201 gcgttgtttt tttgttttgc cgtggaaacg gtgacttgta aacgcgcgga agcgtttcaa
     1261 tccgccttta ccagtacgtt tgaaacgttt tgctgatccg cggtgtgttt tttgttttgg
     1321 catgactata tttcctcctc aaaatctttc gttatccgac tatcggctgt ccaacagcca
     1381 gttagtcatt caaattactt actgtcgttt ttcggtgcca gcgtcaagaa catgctgcgt
     1441 ccgtccattt tcgctttttg ttccactgtt gcaatatccg cagtttcttc agctaagcga
     1501 ttcaagactt tttgaccaat ttctttatgg gtaatggcac ggcctttgaa acggatagaa
     1561 gctttcactt tatcgccctt ttccaagaac ttacgtgcat tacgaagttt tgtattgaag
     1621 tcattcaagt cgatcgtagg acttaagcga acttctttta cattgatcac tttttgtttt
     1681 ttacgcgctt cgcggtcttt cttttgttgt tcgaaacggt atttaccgta gtccatgatt
     1741 cgcgcaactg gtggtttcgc accaggggca actaacacaa gatccaagtt tgcttgttct
     1801 gcaatattca atgcttctgc ttttgtctta actcctaatt gctcaccatc tgaaccgatc
     1861 aatcgtaact ctcgtgcacg aatgccgtcg ttaaccatca tatcctttgc tatggtcatt
     1921 cacctccaaa tattatgaga gaaagaaaag cttaccgaat aaacggcaat aaaaaaacga
     1981 gttctcttac agaacccgcg cgtacttcac ctaatagaac cagtgaaaac tatctagccc
     2041 agtgacatta tcaactaagg cgagaagcgg gtgcttctgc ttttatttct caaccattat
     2101 agtatagcaa aaactaatct ttttgtcaat gtttttcata cagtttgtct tccttttctg
     2161 aaaatgatta gattagcagg ctttctagca gtgattcccg cttctttgct atactaaaga
     2221 aaaacgaggt gaacccacat gaaaaaaatc gaacctattc caccttctct tcctcttctt
     2281 acaaagggag agttttattt agaagatgag gagatcttct ctggtcttct gatcaacgga
     2341 ggtaaccaaa gctatctcga tgccaaaaat atggtattaa gagagagtca tatccaaaaa
     2401 cttacgatgc aaaaaacagg tttacgtcat tttgaatgca gtaatgtcat ttttgaaaaa
     2461 tgtgattttt ccaatctgga gtggattggt ggcagtttcc accaagtcgt gtttcatcaa
     2521 tgtaaattaa caggtacgaa ttttgccgaa agttatcttc gagactgtac tttcactgat
     2581 tgtattgctt cgatggcttc ctttagtagt acaaacttga aagctgtctc cttcgatcat
     2641 tgtcaattag aagacagcga attttatgag gtcacttgga aaaacctttt tttgtctgac
     2701 aatcagttaa ccggttcgaa ctggtttcga acttctctga agggattaga ctttacgact
     2761 aatttcttca caacaatcgc tttatctcaa gattctttgg cgggactaat cgtcaatcaa
     2821 gagcaagcac ttgtgatcgc ccaagccctt ggtttgcaga tcaaagacta gatcaacgaa
     2881 tccgccgcca cagtgcaata agcatccaaa tagataagac caaactgatg atcaaggcga
     2941 tttcccatcc gaaaacggaa tgggtaaagg gtaactggag attcattccg aagaatcctg
     3001 tcacaatagt tggaacggtc aagatcagcg accagaccgt caaaaatttc atcgtgtcat
     3061 tcaagttgtt attcagcaaa ttattataag tgccagagag ctggtctaaa atctgcgaag
     3121 ctaagttcgt catttcaact gcttgtcgtg cttcgatcag tgcatcgtcc aactcttctt
     3181 tttccttttc tgacaacttg cgataaatag ccagtgcctt gatttgttct aaaagtacta
     3241 cattttgttt tgttcctgta actaagtaaa ccaaaccgat ctctaaatct gaaagttgca
     3301 gcagattctt gttcgttgtt ttttctctta atttcagatt aagccgctga cgttcactat
     3361 tcacttcctc gattaaagga aaaaaggaat cagaaatcaa aaacaatgta cgaaacaata
     3421 aactgtatac actcatgtct ggcatccgtt ccaaaagcga tcgaattagt cgaatcacgt
     3481 actcggtatt cggcgtggta aatgtaaaca aaccgtcttc ttttaaaata aaagcgatag
     3541 gactagtttc ataatgatta tcgacttttt gctggtgcgg aacatttgat acaagcagta
     3601 acgcttcatc aaaagaatcg tactctacgc gcgcccgttc atttttatct agagaataag
     3661 tcagcatctc gtcactaatg ccatattctt cttttaagtt tttaacagct agcacatcgt
     3721 tttgctgtac attgatccaa aaattacggt cttgattaaa atagtgtttt tcccgcatac
     3781 tctatcctcc tgacatttgt ttccagtata aagaaaaaag tcgctcaaat aaaggtttac
     3841 cctttatttg agcaactgta aatcaaatgg atactttggt ttggcgaatc tctgctaatt
     3901 tttcttcgat cattttcaag acttcttctt ttgcttgagg atcttccaca aaattatagc
     3961 ggtctccatc gatttggatt tttggacttt ctttgtattc ttcataccat tgatcataac
     4021 gtctgttcaa ttctttgtaa taatcgtata gacttgggtc aaaggacaat tgttcatacg
     4081 aacgaccacg tttttcgata cgctctaaca tcgtatcaaa agatacacga atatgaacta
     4141 ataaatccgg atgtttttta tgggcagcat agggaagttc ttccatcatg ttttccagca
     4201 gttcatcata cacacaaact tctgtttctg ttgctcttcc tagatctgca tttagatgga
     4261 aaagcagtga atcttcataa atcgaacggt ccaatacatt attgtcatct ttcattgctt
     4321 gtttgatgct tgcaaaacgt ttattcaaaa aataaatttg cagcaaaaaa gcatactgtt
     4381 cagggtttgc ataaaataag ggcaagactt cattatcatc aatcgattca taaaatgcct
     4441 gactatcaaa gtgttcagcc atcatttctg ttaaacttga ttttccggca ccaattgtac
     4501 ctgctaaaac gatcacgctc atttacctcc agctcacttc atcactaaat attgtgttta
     4561 actccaacct tttttaaata tacccccaat atctagtagt gtcaattgta ttttttctag
     4621 tcaaagaaga aaaaaagtca aaaaattctt aagaaaaaag tgttttttct ttagaaaacg
     4681 tattcttagt tttacttctg agatggtttt agtaaaataa aggaggaatg ttttttgaga
     4741 cccttagtgg acaaaaaaat tcgtgaagca gcgcaactcg ttttgggcgc tttatttttt
     4801 gcttaaaaat cacttttacg tgagaaggag ttagattatg gcaaactatg gccaaggaga
     4861 atcaagcgga aagatcatat tgatgggcga gcacgcggtt gtttatggag aaccagcgat
     4921 tgcctttcct ttctatgcaa caaaagtcac cgcattcctt gaagagctgg atgcaatgga
     4981 cgatcaactg gtttcttcct actattcagg aaatttagcc gaagctcctc atgcattaaa
     5041 aaatatcaaa aaattattca ttcacttaaa aaaacagcat gacatccaaa aaaacttgca
     5101 actgaccatt gaaagcacga ttcctgctga acgtggaatg ggatcaagcg ctgcagtcgc
     5161 cacagcagtc actcgtgctt tttatgatta cttagcattt cctttgtctc gtgaaatact
     5221 attagaaaat gtccagcttt cggaaaaaat cgcccacggt aatcctagtg gaatcgatgc
     5281 agccgctact agcagcttgc agccgattta ttttacaaaa gggcatcctt ttgactactt
     5341 ttctttgaac atcgatgctt ttttgattgt cgctgataca ggaatcaaag gacaaacaag
     5401 agaagccgtc aaagatgtcg ctcacctctt tgaaacccag cctcatgaaa ctggacaaat
     5461 gatccaaaaa ttaggatact tgacgaagca agcaaaacaa gcaatcattg agaattcacc
     5521 agaaacgttg gcacagacaa tggatgaatc acagtcactt ctggaaaagc tgacaatcag
     5581 caatgatttt cttaatctat tgatccaaac agcaaaagat accggggcat tgggtgccaa
     5641 attaactggc ggtggacgcg gtggctgtat gattgcatta gcacaaacaa aaacaaaagc
     5701 ccaagaaatc agtcaagcac ttgaagatgc gggtgctgct gaaacttgga tacaaggatt
     5761 aggagtacat acctatgttt aaaggcaaag cacgcgcata taccaacatt gctctaatca
     5821 aatattgggg taagaaaaat gaagaactca tccttccaat gaacaacagc ctttcgttaa
     5881 cattggatgc attttataca gagacagaag tcatcttttc tgatagctat atggtagatg
     5941 aattttatct agatggcacc ttacaagacg aaaaagcaac aaaaaaagtc agtcagtttc
     6001 ttgacctttt tcgtaaagaa gccggcctct cactaaaagc ttcagtaatc agtcagaatt
     6061 tcgttcctac cgcagctgga ttagcctcct ctgccagcgg gctagctgct ttagcaggag
     6121 cttgcaatac tgctcttaag cttggattag acgatctctc tctttcaaga tttgctcgac
     6181 gcgggtctgg ttcagcttgc cgaagtattt tcggtggttt cgttgaatgg gaaaaaggcc
     6241 atgacgactt aagttcttac gctaagccag tcccttccga ttctttcgaa gacgatttag
     6301 caatggtttt cgttttgatc aacgaccaga aaaaagaagt gtccagcaga aatgggatgc
     6361 gtcgaacagt cgaaacatcc agtttttatc aaggctggtt agattccgtt gaaggggatc
     6421 tatatcaatt gaaacaagca atcaaaacaa aagatttcca acttctcgga gaaacgatgg
     6481 aaagaaacgg actaaaaatg catggaacga ctttagctgc ccagcctcca ttcacttact
     6541 ggtctcctga cagcttaaaa gcgatggatg cggtgcggca attgagaaaa aaaggcattc
     6601 catgctactt tacgatggat gctggcccta atgtcaaagt tttagtggaa aacagccatt
     6661 tatctgaggt acaagaaaca tttaccaaat tatttagcaa agaacaagta attactgccc
     6721 atgctggtcc aggaattgcg attattgaat aaagggagat ttttatgatt gaagtatctg
     6781 caccaggcaa actctatatt gccggagaat atgcagttgt tgaaacagga catccagcag
     6841 ttatcgctgc agtcgatcaa ttcgtaacag tcactgtaga atccgcacga aaagtcggaa
     6901 gtatccaatc tgcacaatat agtgggatgc ctgtacgttg gacaagacgc aacggagaat
     6961 tagttttaga tattcgggaa aatccttttc attatatcct tgctgctatt cgtttgactg
     7021 aaaagtatgc acaagaaaaa aacattcttt tatcctttta tgatctgaaa gtaacaagtg
     7081 agttagacag ttcaaatggc cggaaatatg gattgggttc tagcggtgcc gtgaccgttg
     7141 caacggtcaa agccttgaat gttttttacg cgttgaattt atctcaattg gagattttca
     7201 agattgcagc actagccaat ttagcagttc aagataatgg ttcctgcggc gacatcgctg
     7261 ctagctgtta tggtggctgg atcgctttct caaccttcga ccatccttgg ctccaagaac
     7321 aagaaactca gcattctatc agtgagttac ttgccctgga ttggccaggt ctatccattg
     7381 agccattgat tgctcctgaa gatttacgtt tattgattgg ttggacgggt agccctgcct
     7441 ctacttctga tttggtcgat caagttcacc gttcgagaga agacaaaatg gtggcttatc
     7501 agcttttctt aaaaaacagt acagaatgtg tcaacgaaat gatcaaaggg tttaaagaaa
     7561 ataatgtaac gttgatccaa cagatgattc gaaaaaaccg acaattactg catgatttat
     7621 ctgcaatcac tggggtcgtc atcgaaacgc ctgctttgaa caaattgtgt aatttagctg
     7681 aacagtatga aggagccgca aaatcttctg gtgcaggtgg tggcgattgc ggaatcgtaa
     7741 ttgttgacca gaaatctggc atccttcctt taatgagtgc atgggaaaaa gcagaaatca
     7801 ctccactgcc gttacatgtc tatagcgatc aaagaaagga aaaccgatga atcgaaaaga
     7861 cgaacatgtt tcactagcta aagcctttca tgataaacaa aaaaacgaat ttgacttcgt
     7921 acggatcatc cataaccctt tgccgcaaat agcagtttct gatgtcgatc taagtacaca
     7981 ggcagttggg tttacactaa gcagcccatt ttatatcaat gcgatgactg gcggaagcga
     8041 aaaaacaaaa aaaatcaacc aagatcttgc aatcgtcgca agagaagcag atttgatgat
     8101 tgccactggt tcagtcagcg ctgccttgaa agacccttct ttagccgata cttatacgat
     8161 tatgcgacaa gaatatcctc acgggaaaat cattgccaac atcggtgcag ggacttctgt
     8221 cgaaagagcc caagaagcta tccgattgtt tcacgcagat gccttacaga ttcatttgaa
     8281 tgcacctcaa gagttggtta tgcctgaggg agaccgtgat tttaccaatt ggaaagtact
     8341 tattcaagag actcaaacag ccattgacgt ccccctcatc gtaaaagaag tcggttttgg
     8401 catgacaaga gaaacgctca atgatctagc tgctttagga gttcatacag ttgatatcag
     8461 cggtcgaagc ggaacaagct tcacccagat cgaaaacgcc cgtcgctcca agcgggaact
     8521 gagttattta gctgactggg ggcaatcgac agtctcttca ttgcttgaag caaatgaagc
     8581 agacacttct atggaaatcc tagcctccgg aggtatccgc aatgcctatg acatatttaa
     8641 agccctttgt ctcggcgcaa atgcagtggg tacttcagga accgtactga cgcatctgat
     8701 gaatcacggc gtagaagaaa ctattatact gatgaagcaa tggcaagaag agcttcgtct
     8761 gctttatact atggtcggcg caacgaatac cgcggcccta caccaacaat cactgatctt
     8821 ttccggacca gtgaaggatt ggtgcgaagc aagagggatc gacttagtga agtatggaag
     8881 acgcacagaa aagagcgtgg gacaaaagta aagaacactt ttatcccacg ctctaaacac
     8941 gtataaacgg cggaagca
//
LOCUS       DBNIBO010000113         8291 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.117, whole genome shotgun sequence.
ACCESSION   DBNIBO010000113 DBNIBO010000000
VERSION     DBNIBO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.117"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            674..1900
                     /locus_tag="AC1Y77_002131"
     CDS             674..1900
                     /locus_tag="AC1Y77_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1575682.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2058..2675)
                     /locus_tag="AC1Y77_002132"
     CDS             complement(2058..2675)
                     /locus_tag="AC1Y77_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1575683.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(3358..4353)
                     /locus_tag="AC1Y77_002133"
     CDS             complement(3358..4353)
                     /locus_tag="AC1Y77_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1575684.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(4448..5320)
                     /locus_tag="AC1Y77_002134"
     CDS             complement(4448..5320)
                     /locus_tag="AC1Y77_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIQ1575685.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(5333..6760)
                     /locus_tag="AC1Y77_002135"
     CDS             complement(5333..6760)
                     /locus_tag="AC1Y77_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIQ1575686.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(6814..8088)
                     /locus_tag="AC1Y77_002136"
     CDS             complement(6814..8088)
                     /locus_tag="AC1Y77_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1575687.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         2746 a         1524 c         1185 g         2836 t
ORIGIN      
        1 attttaaatt tttacatacc tgtttatgaa aaactaaata atctgtcaaa aaaatatgat
       61 tgttcatttc taaatatgtt ttccaatttt aaaatctttt ttagcatttt tgtttccttt
      121 tcatatggtt tttcgttgtt aatagtgact aggatgatat tcctattttg accattgaca
      181 attaaatata atttttgaat aacgttccct gctattcgag ctaagaattc aagtacgcca
      241 tgacctattt agcgataact acttagagat ttctttttgt tttgggaaga caaaatgcca
      301 tgacaatagc agtagataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
      361 aattcctatg tgcttaccca agtcaattca aaaattttag ttcatttata aggggatgat
      421 tttattaaat caatagtcta tctaaacaaa aaacaaacct gtgagttttt aggaattgat
      481 aacaatactt tagataaatg gattcgtata tataattttc ccgtaataaa aatagacggt
      541 ataattcgct ttgatgtaga agatatcaaa ggctttatgg atacgttcaa aaagatttga
      601 attttacttt tgactgcata tactaaaagt agcgaaatat actgccttac ttttttatta
      661 aggagtgagg aagttgccta cgaagctttc aaatggaaaa tataaaacta atttgagata
      721 tccaaaaaaa tttaaagaaa ttacaggtat cagttcagaa aaattccaaa aaacatttcc
      781 aactaggcaa ttggctatta aagccgaaaa tgaaatgaaa aagaaaatcg caaaagtctt
      841 aagagaggag aatgcaaatt cattagagct caaaggtcaa ataaaattca aggagtttta
      901 taaatctaaa tggcttccta gatatgagtt agggcaaact actagatccc ataaagctcc
      961 aagcgatata accatcatta atacaaagaa tttatttcga ttgcatattc tgcctatatt
     1021 tggtgagtat gccatgagct atcttaatgc aaataccgaa ataatatcag atgaactcac
     1081 aaaaaagtcg aaggaatatg caaacataaa aatcattaaa tgctatgttc gttccatgtt
     1141 tgatattgca gaggttctaa attatattga atttaatcgt acaacaaaaa taattcaaag
     1201 tattactgca cctaagaaag ttgctctgga agaaaaaaga atacgagaag gaaaacaagc
     1261 tttatcttcg aaagaactca ctgattggtt agacgctgta agagatgatt tcaatgatca
     1321 attactttcc tttcacgatt acactctttt tatgcttact ttatatcttg gggatagaaa
     1381 gagtgagacc tatgctttgc aatggaaata cattgatttt gaaaaccaaa cagtccgtct
     1441 taaatatgcc ttggataaac acacaaaaaa gaaatttaca aaaggtcgaa aagatactat
     1501 tatccaagtt ccggaaattg taatgacact attgcaagaa tggaaatcag tgcaagctga
     1561 acaactattg aagttgaaaa ttaaacagac acctgaccaa tatcttttta gttactccaa
     1621 accttctggt gaagtaaatt gtccagtcca tactgattac ttaaattatc gaattaattc
     1681 tataaaaaaa agacatcccc atttggttca tttaactcct cataaacttc gacatactta
     1741 cgcaacttta gctagacaag gtggggctga tatgaatcaa atttctaacg ctttgacaca
     1801 ttcaggtatt tcaactacaa aaatttatgt gaatacacct gatttagttg ataaagctgt
     1861 gttcgaagcg tttcagcgag gattacaaaa atctaattaa tgtgttctag aatgtgttct
     1921 aaatataaaa aaaagacact ttccaaataa ttggaaagcg tcttgaacgt tgatatataa
     1981 aggatattaa cgttttgaga attgagatgc tttacgagct ttcttaagac ctggtttctt
     2041 gcaaaattat attagcctca actgaaagat ttattttttt gctccctaac ttcattagta
     2101 ataccatgtt tcattcgata tcttctaaac gttctacgat taatccctgt tagctcttca
     2161 acttctttat ctgtagatcc agtcaaaaac aaattaaatg ctaattgaag tcgcggatca
     2221 ttctcacgat attttagcgt acctcccttg tatttccctt ttgctttagc gagtgcaatt
     2281 ccttgttgtt gcgtttcgcg aatataccga cgttcttctt cggcctggta cttgtatagt
     2341 tcgataatta agttattaag taatcgacgt aaattatcat cagtgatacc tgttaacgct
     2401 ggcaaattca aaacttccag tgtagctttc ttcagctgaa ttaaattcat cgtttctgtt
     2461 agctctttat tatttcgtcc aagtcgtttt aattctgtca caacgacaat atcaccttca
     2521 cgtatgaatt tcatcatttc ttgaaatcct tctcgattgg tatctttccc acttgcttta
     2581 tcactaaaaa ttttatcaca ctctgataat aattctaatt gtcggtctaa attttgatct
     2641 aagctgctta ctcgagcata cccaattttc gccataataa aactccttta aaattctgaa
     2701 atccggatca tttttaaatt ctgttgccca actctgttgt catttttcta gataaatggc
     2761 ataaattttc agcgctaagt gacaaaagca actaattttt catgatagat ttttaaatat
     2821 ctcaatctat ctccaaaatt agttgctttt gtagctatag ttgactatga atatgacaaa
     2881 taattagaaa aaactgtgcc aattaaatat aaaaatgaca ctcatttaca ttcaatagaa
     2941 caccaagagc ccaacagggt atactctggt gtccttttaa aaaagtaact ttctgaccta
     3001 attaattgag ctactaaaaa agctatgaaa tgaggaataa aaaagggatc aaaaactttt
     3061 aagtttctga cccttttgag ttctaattat ttcttccaga acgaagaatg ttatctgctt
     3121 tatctgcaat tgattctgtt agaaggactg aaaaatttat gccagccctt ttgcctagct
     3181 tatccgccca ttgaggaatt gttaaggttt ttctgacaag ttctatgtct ttcaaatatt
     3241 tggcaacatc tacacagatt aaaagcatga ttttgtatca tagtctatta taagagattt
     3301 aacaattttt tatatcacga attaataatg aaattatcat tgatagtctt tataaattta
     3361 attgttcttt ttctttactg atgtcttccc catccataat gtgacaggta aaatatattc
     3421 cttttttgtt tcttctttat ttattcgttt catcaaaata gcaacagctg tgtctgctat
     3481 atcattaatt ggttgtatta ctgttgtcag atctggtaat atactccgag tcattaaagt
     3541 accatcatat ccgactacta ataaatcagc tggtacattt aacccctttt cttttgctac
     3601 ttgtaatatt agggccgcat cgatgtcatt tgaagcaaaa ataccatcta ttttaggata
     3661 atcctcaaac atttggaaaa aaatctgttt tttctttaca taagaaatat tgaaatctaa
     3721 tgtcacagtt cgtggaatta actgatatgc tttcatagta tcttcatagg ctagtctccg
     3781 acgattagcc ggagtctgta agtcaattgg accgtttgta tgaataatat tttttgcacc
     3841 ttgtgcaatt aataacttgg ttgcaagttt tccaccattg tagttatcgg attcaacaac
     3901 tggaatatct tcattcatca ctcgatcaat tgcaacaatc ggtaaattct gatatttata
     3961 ttcctgaatt ccttggttat gtgtaccaac aattaaacca tctacttgat cagacaataa
     4021 ctgatttaaa taatttgttt ccttttcttt attattcatc gaattgccta ttaatacctt
     4081 ataaccaatt tcatataact ttttttccaa tgcctcaacg agttcactaa aaaaaggatt
     4141 ggctactgta ggaaatagta acccgacaat gttagttttc ttttgaaata attgacgcgc
     4201 tacaacgttc ggttgataat tgagttcttc tatagcctta taaacattat caatcgtttt
     4261 ttgacttaaa tagccacgat tgtttaatac acgtgaaaca gttgtttttg atacatttgc
     4321 tcttttggca acatcttcta attttgggcg cattctcttg tctctcctaa ataatgcaat
     4381 aattaactat cgaaaaaaag aactctatag aactactata cctgataatc gatatttttt
     4441 ctatgtttta ttcttgtaat tgattcaagc ccaaagcaaa gtttcctagt gttgccgatc
     4501 cattattttt aattaaagga cgcgtaatat atttacttaa cggtggtatt tccacgtaat
     4561 catccaataa tttattaaca ttaacacgaa ttttttctaa caatttttca tttactacgc
     4621 ttccaccaaa tactatttta tccggtcgta taatcaatgt tgtttgaatc acagcttgtg
     4681 ctagataata ggccattaca tcccaaatag gatccgttaa agaaacgtct tcaccctttt
     4741 ttccaaaacg tgcttcaaac gttggtcccg caactaagcc ttctaaacaa tcaccatgat
     4801 aaggacaaat tcctttaaaa tcaatatcat caggatggcg ttttacaaac gtatggccca
     4861 tttctgaatg accaaacgat cctaatagtt ttccgtcaac aacagctcca ccaccaacgc
     4921 cagtaccaac ggtgtaataa actaatgaat ttattttttc attcgataaa gtggacatga
     4981 tgtactctcc ataagctgat ccgttcacat ccgttgtcca agacattgga acattcagct
     5041 catcttgtaa ataacctaac acattaacat ttcgccatcc cacctttggt gtagacgtaa
     5101 tatatcctga ttttggtgaa ttttttcgaa tttctattgg accaaatgat gcaataccta
     5161 atgcatttat atcaaactgt ttaaaatagt caacagtttt tttcaatgtt tccctggggg
     5221 tggtcgttgg acaaattatt tggtcttgta ctcggtaatc ctcgtctccc acggcacata
     5281 caaatttagt cccacctgct tctatgctac caattaacat cataaaacct ccttactcgt
     5341 aagaaattgc atcttttttt agatgataaa ttgtactcga gacaaaagct tcttcactaa
     5401 cactcaaatg catacttggt tgattttctg tataataacg ctctgtaaaa acaagctcgc
     5461 catcattaat aaaaatctct gccgaacttt tatcgataaa cactcgaagt gataatctat
     5521 cacacatctg aatggttcca taccttggat catttttgcc gttacgagtt aaggtacatt
     5581 catttttatc cttagaataa aaaatattga ttaactcttc atcttttgtt tttaatgaaa
     5641 atttgacttc ttgatttttt gatttagtca atagtacatc aattagagct tctttgaatg
     5701 aagcatcttg agcgattatt atttctcctg ccaaattaac tgtcgtttca acgccgctgc
     5761 caactcgaag ttgttctaat tctctcacag gtttcatata caaacgatct ccaattaact
     5821 ctaattctcg aggaagcgtt aatgctcctg cccatccgtc tactttttca ggcatagggc
     5881 tttcccacat atccatccag gccataacaa tacgtctacc atctggagat aaagttgttt
     5941 gtgttgcata gaaatcgtgt cccttatcta attcataaaa atttcctcta atgaattttg
     6001 ctgtctcata atcataatca ccgataaagt agcctgtttc atatagattt aaataatctt
     6061 ttccatttgc ttcaattccc tgaggggata ataaaagaat agattttcca tttaattcaa
     6121 aaaagtctgg gcattcccac ataaatcctt ctgtaagtac ttccttcgct ttagaaattg
     6181 gtccaatgta atcccaattt aataaatcta acgactgata aacaatagca cgacctaatc
     6241 catctttttc ttgactaccc aaaaccatgt aatatttttc atcgtgtttc catacttttg
     6301 gatctctgaa atgatgtgtg ttatcttctg gtgcttttgc tattacagga tttttttcat
     6361 atttagtaaa gtgaatgcca tccgtactat acgccatgtt ttgattttgc caaaaatgat
     6421 ctggatcatt gtctccataa tagtgatgtc ctgtatagaa cagatacaac acaccatttt
     6481 tttcaatagc gcttcctgaa aaacaaccac cttcatcttc ttgatctcca ggagtcaaag
     6541 caagtggtaa agattcccaa tgaactaaat ccttacttct agcatgtgcc cagtgcattg
     6601 gtccccattc agcactatat ggatgatgtt gataaaacac atgataatac ccatcaaaat
     6661 agcaaaatcc atttggatca ttaatccatc ctgatggtgc agacacatga tatcccaaac
     6721 gataacgtgt atttgttaat tctatagctc tttttgtcat aatatattcg ctcactttct
     6781 agataaaatt gttttttatt ctgttggttt ccattatcct cgaataaatg gatccccttg
     6841 aacatctcta tcatcttgtt taagaataaa gaatgcatat atgcctgcta gtaatacaat
     6901 aagagaaata attttaaatg ttggttgata gccaacgttg tctcttaata ttcccaatgg
     6961 tgttgataaa atcacttgcc caatttgagc agctatctga aatcctatca tatagagggt
     7021 tgctgatagc tttgtatcaa aatgaagtgt aaaatagcga aacattggta atgtaaataa
     7081 tggtacttct aaagcatgca acatttttat gcatgaaaca gataatggtg tcgaggcaaa
     7141 cccgcacaat ccaattcgta cacacataat ggttacacct aataacaaag tatttcgaac
     7201 ccctaatttt ctcataatga ttggaacaat tcccatcatt aacgcttcac aaaatacttg
     7261 aatagcattc aatgtcccat atattttttc accattagct gatgttgaaa acaaaccagt
     7321 ataaaatccc ggaaacattt gttgatcgaa taccgtataa aatgtccatg taaaaacaat
     7381 aaagatgatt atcgtccata attgaggcag tttcattaga tcaaacattt cttttaaaga
     7441 aggaatacta tttgattcag cttgttcttg attaaaattt tgatttgctt cccgttcaac
     7501 tttagggttc caaaataata aatttaacaa tagtaaagaa ccaaagaaag atcccgccca
     7561 aaagtttaaa tgaggatttt ttacaaataa aaatcctgcc aaaagcgcta ccaaggcata
     7621 accaaatgat ccccacgccc ttgcttgtcc atattcaaag ccaaataaac gactaaacct
     7681 ttctgacaca gcttcaaaaa ctcctacaga agctaaatat ccagctgaca aaaatagcgc
     7741 tcccataata atgcctaaat taaaattgtt atctagcaat ggcccatata tccatataaa
     7801 aaatggtcca acaagtgtcg ctaatacaga agcaaaaatt aataaatttc gtttaatata
     7861 caatttatct tgtaatgttc cataaataaa catcaaaatt aaggtaacta acgaatttgc
     7921 cgagaatact gttcctacag cactgccaga taaccctaaa ccatttgatt cagatgttag
     7981 ccaaagttga aaaaatgacc accaaacacc ccaagaagca aaaaatagta ataatgtagc
     8041 tgaactttgt aaataagatt gatttttaaa tacgttaact tttgacatat ttttatcctc
     8101 caacgatata tttgtatccc ctttcatgat aaccggttaa catttttaga aaaggacttg
     8161 tactcctaaa attataaaag aaatccgctt acatgtcaac cggttattct atttggataa
     8221 tgccatataa tgtcacagga tatgactttt attggtgtgt cgcttttctt ttttaatggc
     8281 actatttttt a
//
LOCUS       DBNIBO010000114         8244 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.118, whole genome shotgun sequence.
ACCESSION   DBNIBO010000114 DBNIBO010000000
VERSION     DBNIBO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.118"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     regulatory      96..175
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            173..1366
                     /gene="metK"
                     /locus_tag="AC1Y77_002137"
     CDS             173..1366
                     /gene="metK"
                     /locus_tag="AC1Y77_002137"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIQ1575688.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1496..2968
                     /locus_tag="AC1Y77_002138"
     CDS             1496..2968
                     /locus_tag="AC1Y77_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIQ1575689.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3051..3839
                     /locus_tag="AC1Y77_002139"
     CDS             3051..3839
                     /locus_tag="AC1Y77_002139"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIQ1575690.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3906..4574)
                     /locus_tag="AC1Y77_002140"
     CDS             complement(3906..4574)
                     /locus_tag="AC1Y77_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIQ1575691.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4689..6401
                     /locus_tag="AC1Y77_002141"
     CDS             4689..6401
                     /locus_tag="AC1Y77_002141"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575692.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6404..8176
                     /locus_tag="AC1Y77_002142"
     CDS             6404..8176
                     /locus_tag="AC1Y77_002142"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575693.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2524 a         1383 c         1811 g         2526 t
ORIGIN      
        1 cttttttgga cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa
       61 atacgagcag aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat
      121 ccgcaggacg gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga
      181 aagacactta tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca
      241 aatcagtgat gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg
      301 tgaaacgtct gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata
      361 tgtagatatt caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa
      421 attcggattt gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga
      481 tattgctcaa ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt
      541 agatgagatt ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc
      601 agaattgatg cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt
      661 gcgtaaatca aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga
      721 atatgatgat caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga
      781 tgatgcagta gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt
      841 tattccagct gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt
      901 tgtcattggt ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac
      961 gtatggcggt tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt
     1021 tgaccgttct gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct
     1081 tgctcgcaaa gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat
     1141 ctcaatcaat actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag
     1201 agaaaacttc gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat
     1261 ttacaaacaa acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga
     1321 acatacggac aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg
     1381 gaacgatcgc caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa
     1441 gatagtaatt atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa
     1501 aaaagaaaca aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc
     1561 tattgaaggt acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat
     1621 ggaaatcatg aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat
     1681 ttacggaaaa cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt
     1741 gtttattatc ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc
     1801 tcgtgcgatt caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg
     1861 cgatctttat tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg
     1921 gattgccagt gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca
     1981 ctggatcttt tttatcaatg tgccgatcgg aattctatta attattttga tttggctata
     2041 tttaaatgaa ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag
     2101 tttgatggcc atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat
     2161 gagtccgctt gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt
     2221 tgccgaaaaa agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt
     2281 tgttgtggtc aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta
     2341 tatccctatg tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt
     2401 agcaccatta tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca
     2461 aatgaaaact cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg
     2521 gttgtatctt atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat
     2581 cggtataggc ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc
     2641 tcgtgaccag cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac
     2701 agtcatggtt tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc
     2761 agcaaattta acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa
     2821 atatattgat gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc
     2881 cgtttatcta gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca
     2941 tgaaaaaagt ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat
     3001 tcaaatttaa agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta
     3061 ttcaagcaaa tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac
     3121 cgcaaacaac aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg
     3181 ttttatattt acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga
     3241 ttgaacgtta tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct
     3301 ggtacacaga tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg
     3361 agatttgtca tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg
     3421 gattatcgat gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg
     3481 cagcagtaat ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga
     3541 tggttcgtaa agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt
     3601 caaaaaatga tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa
     3661 gattttatat ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat
     3721 ctgctatgca ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact
     3781 ggatcttctg ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa
     3841 aaaagagtct taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag
     3901 cttttttact gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt
     3961 tgtgcactcg ttagaaagag tgctttttca ttcaattctg catgggactg catactttct
     4021 ttaaatccct ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc
     4081 aaaaattcat ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt
     4141 aactgtccaa tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa
     4201 aatccttcac gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa
     4261 gcgggcacag tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag
     4321 taattttcca tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct
     4381 aattcttctt ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac
     4441 caatataaag caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc
     4501 gaaagagctt gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa
     4561 agctttattt ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt
     4621 gttagtcggt ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta
     4681 atcaacgaat gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt
     4741 ccttgctgtc cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc
     4801 aagtattgga agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc
     4861 tgatcagtct ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa
     4921 aagtagctca aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat
     4981 tttcatttgg aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg
     5041 atatcacgca aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat
     5101 tcttatttat tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca
     5161 tcgtagcgct tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca
     5221 agcacttcat gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact
     5281 tactagggat tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca
     5341 gcaaagtaag tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga
     5401 tgatcccgtc cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga
     5461 gcgatcttgt taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact
     5521 tgatgcagat tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag
     5581 cagccgtatc gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt
     5641 tagacgttcc agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt
     5701 atcctggtga ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa
     5761 tggttggtat cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc
     5821 gtctatttga tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa
     5881 atgaaaagag tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag
     5941 gaacgattgc tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac
     6001 atgcttcttc tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag
     6061 caccaataga agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta
     6121 ctcgtggcgt tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg
     6181 atgctcgctc tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga
     6241 cgattgtcat cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg
     6301 atgaaggcag attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct
     6361 atcaggaaat ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa
     6421 agcaagtaaa tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt
     6481 tatcacagtg atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc
     6541 ttttcaagaa ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa
     6601 atttgtttct gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt
     6661 tatctatagt attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg
     6721 tctattcaac aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga
     6781 aattttaagt cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt
     6841 gcttcaagtc atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca
     6901 aaatgtagag ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt
     6961 cttgattatc cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact
     7021 aaatggttat atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca
     7081 agaagaaacg attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa
     7141 aggacaagct tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac
     7201 agcgatagtt atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt
     7261 ggcattaggc ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat
     7321 gcagatttca tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa
     7381 tgaaatattt gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt
     7441 tgaaaaaggt cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt
     7501 gaaagatgtc tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg
     7561 atcggggaaa acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc
     7621 agtagtcatc gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt
     7681 aggaatcgtt ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt
     7741 cggcaaaccg gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca
     7801 tgacttcatc atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt
     7861 attcagtaca ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc
     7921 tttattgatt ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca
     7981 aaaagcaatg gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa
     8041 aacaatcttg aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg
     8101 cagtcatcat gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt
     8161 tgtatttgaa agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta
     8221 tgttgtttgt ttttataagg ggca
//
LOCUS       DBNIBO010000115         7944 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.119, whole genome shotgun sequence.
ACCESSION   DBNIBO010000115 DBNIBO010000000
VERSION     DBNIBO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.119"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            382..1755
                     /gene="glmU"
                     /locus_tag="AC1Y77_002143"
     CDS             382..1755
                     /gene="glmU"
                     /locus_tag="AC1Y77_002143"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIQ1575694.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1849..2820
                     /locus_tag="AC1Y77_002144"
     CDS             1849..2820
                     /locus_tag="AC1Y77_002144"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIQ1575695.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2826..2928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2994..3563
                     /gene="thiT"
                     /locus_tag="AC1Y77_002145"
     CDS             2994..3563
                     /gene="thiT"
                     /locus_tag="AC1Y77_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIQ1575696.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3644..4417)
                     /locus_tag="AC1Y77_002146"
     CDS             complement(3644..4417)
                     /locus_tag="AC1Y77_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575697.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4684..6384
                     /locus_tag="AC1Y77_002147"
     CDS             4684..6384
                     /locus_tag="AC1Y77_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIQ1575698.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            6354..6572
                     /locus_tag="AC1Y77_002148"
     CDS             6354..6572
                     /locus_tag="AC1Y77_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIQ1575699.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6592..7482
                     /locus_tag="AC1Y77_002149"
     CDS             6592..7482
                     /locus_tag="AC1Y77_002149"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIQ1575700.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            7500..7784
                     /locus_tag="AC1Y77_002150"
     CDS             7500..7784
                     /locus_tag="AC1Y77_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575701.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2693 a         1364 c         1669 g         2218 t
ORIGIN      
        1 agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc
       61 ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat
      121 gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc
      181 ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt
      241 accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa
      301 aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa
      361 ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa
      421 ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg
      481 gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt
      541 ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa
      601 gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa
      661 aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac
      721 aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca
      781 gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc
      841 gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact
      901 tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac aaacaatgct
      961 caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt
     1021 gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta
     1081 gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta
     1141 agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca
     1201 ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc
     1261 gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca
     1321 gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga
     1381 ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct
     1441 caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga
     1501 gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat
     1561 catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc
     1621 gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa
     1681 tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc
     1741 ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta
     1801 tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat
     1861 tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa
     1921 attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga
     1981 gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca
     2041 acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa
     2101 cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac
     2161 cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa
     2221 gctggtgcaa caagaatgct gactttggac cttcatgcag ttcaaatcca aggtttcttt
     2281 gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa
     2341 ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca
     2401 cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa
     2461 gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg
     2521 atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa
     2581 gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta
     2641 caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca
     2701 gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca
     2761 atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa
     2821 taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt
     2881 cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt
     2941 cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa
     3001 aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga
     3061 ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt
     3121 ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac
     3181 attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga
     3241 ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg
     3301 tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac
     3361 tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat
     3421 gggggatgaa tccttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag
     3481 ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc
     3541 cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt
     3601 cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat
     3661 aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag
     3721 tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa
     3781 tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa
     3841 tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa
     3901 caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac
     3961 ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca
     4021 gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat
     4081 gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt
     4141 ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt
     4201 tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg
     4261 tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa
     4321 aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac
     4381 ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc
     4441 ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat
     4501 tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt
     4561 tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa tctacaaagt
     4621 cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa aaaggagagc
     4681 gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc gcctatccag
     4741 caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga cgctttttgg
     4801 cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac cgtctatttt
     4861 actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga aggtgaaact
     4921 tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt tattaaagaa
     4981 aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat ttttcataaa
     5041 aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac agcacttgtt
     5101 ttaggtggag gaggagcgca cggtgcttat cagattggtg tgtggcaggc gctgaaggaa
     5161 catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa cggcgcattg
     5221 attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc gacaagacaa
     5281 gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt ctatcgagaa
     5341 agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc tccattggaa
     5401 aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa aatacgttta
     5461 tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat ccaacagaca
     5521 aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc cgctatggct
     5581 tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa aatacctatt
     5641 gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca agggccaggc
     5701 ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca aacattgtgg
     5761 acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt acaattaggc
     5821 tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac atttcattct
     5881 gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa agaaattcag
     5941 ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa tttatataag
     6001 aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc aaaaaaacat
     6061 tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat ctgccaacaa
     6121 gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag tactgaagaa
     6181 tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa aactgtttat
     6241 tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt gcttaagcaa
     6301 ccagttgaca cattactgat cctatattta tattatctca aggaggaaca accatggcac
     6361 aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac aacaaggggt
     6421 ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc gatcttcgag
     6481 attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac gaagagtttg
     6541 aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg aatgaaaagt
     6601 atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga tattgaaata
     6661 aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat cctgaacttt
     6721 gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg aatcgaagta
     6781 tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa ctgggaattg
     6841 attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg tagtcaaaat
     6901 caagtgtcaa ttagtcggat ggcggaacgc ttcgcgctgg acgaaaactt cgtaacggta
     6961 gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga tacattgaaa
     7021 agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt agctttagtg
     7081 ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag ttttgtagaa
     7141 atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt tcttggagcg
     7201 aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg cggagatatt
     7261 cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact tatcgaagca
     7321 caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg ggaaatcaac
     7381 acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca gtctaagacg
     7441 atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag gtagagataa
     7501 tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt aaacgggata
     7561 tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac tataatgaga
     7621 acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg aaatttattg
     7681 ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct cgaaaagatg
     7741 agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga tgctgaacaa
     7801 agaaatacaa taaataatca gaattggatt tcttcgctta gcttctttgc tctaggaata
     7861 catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa taagtgatgt
     7921 tcaaaaacca ttttctcggt atta
//
LOCUS       DBNIBO010000116         7790 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.120, whole genome shotgun sequence.
ACCESSION   DBNIBO010000116 DBNIBO010000000
VERSION     DBNIBO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.120"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            106..390
                     /locus_tag="AC1Y77_002151"
     CDS             106..390
                     /locus_tag="AC1Y77_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575702.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            393..1310
                     /locus_tag="AC1Y77_002152"
     CDS             393..1310
                     /locus_tag="AC1Y77_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIQ1575703.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1303..2085
                     /locus_tag="AC1Y77_002153"
     CDS             1303..2085
                     /locus_tag="AC1Y77_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1575704.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2086..2766
                     /locus_tag="AC1Y77_002154"
     CDS             2086..2766
                     /locus_tag="AC1Y77_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1575705.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            2759..3655
                     /locus_tag="AC1Y77_002155"
     CDS             2759..3655
                     /locus_tag="AC1Y77_002155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1575706.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            3850..5499
                     /locus_tag="AC1Y77_002156"
     CDS             3850..5499
                     /locus_tag="AC1Y77_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIQ1575707.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            6093..7028
                     /locus_tag="AC1Y77_002157"
     CDS             6093..7028
                     /locus_tag="AC1Y77_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575708.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            7021..>7790
                     /locus_tag="AC1Y77_002158"
     CDS             7021..>7790
                     /locus_tag="AC1Y77_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575709.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         2406 a         1350 c         1717 g         2317 t
ORIGIN      
        1 aagaagggat tgtgaaaagt cttttgtcat aacctcttat ttggtagcta attgtaaatc
       61 ccaaacggca tgtattattt ttcataagtc cgtttttaag gattaatgtt ttcttttgct
      121 ttttatatgg tctggcttca gcgagaagtt ccaacgcata tacaaggacg tctgatgtca
      181 acgataacaa cgattaccat ggtggccagt ccacttggat tagcactgtt tggtgcgctc
      241 tttgattggc agacaactca accacgtctg cgagacagtc tgatttttct tgaaattcaa
      301 ttggaaaatt tacactttct ttatacattt ctggctgctt tctttatacc cgtcaactat
      361 acttcactta tcaataagaa agaaggataa gtatggattt tggaaatgaa acggtcttga
      421 aagcaactgg tataaccaaa aaatacggtg ctgccaaagc attagataaa gtatcgatcg
      481 aaatcaaacg tggaatgatc tatggtttga ttggggagaa tggagcgggt aagtctacct
      541 ttatgagaac tattatgggt ttgatctcga ttgatgaagg ttcgatcgaa ttatttggaa
      601 cgacggattt gcaggcagca agacgacgta tgggtcaatc gattgaaacc cctgctcttt
      661 atccagaatt aactgcaaga gataacctac gtatccaagc agcgaacggc ggtgtgtcgg
      721 atagagaaat cgaagacctg ctgaaaatga tgcgattgga aaatacaggg aaaaagaaag
      781 caaagaattt ctctttaggt atgcgccagc gtttagctat tgcaaatgca ctgatcacga
      841 atccagaatt tttgattttg gatgaaccga cgaatgggat ggatccagca gggatggcag
      901 aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg catcaccgtc ttgctttcta
      961 gccacttgct agatgaattg tcacagatcg ccactcacta cggtattttg catgaaggac
     1021 acttgatcaa agaattatcg aaagaagaat tggcacaaga atctcgtcaa tttataaaaa
     1081 ttgacacttc ggcgacggaa caagcagtaa cggttctcga ctctcttggt tatagggatt
     1141 attttgtcca atctagccgt gttatccagc tttttgaagg aattgaccaa gtagcagcca
     1201 tcaatcaagc attggtagag gcaaaagttc ctgtggatgg tatccaccta gttgggcaaa
     1261 aactggaaga ttatttctta caattaactg gaggcaatcc acatgtttaa tctattaact
     1321 gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg tctcactagg actgatggtc
     1381 gtacttccgc ttttttattc ttggaacgaa tggtatatgc atacaaaata cggcaatgaa
     1441 ttaaaccaag caacggatac agtcatcaac ggtgcaacag ggattttaat ggtggaaaaa
     1501 atggctgcat ggattcttct ggctttcgca gcatttgcgt gcttttatat tggggaagaa
     1561 tttcaaaacg gtacgattcg caatacgtta tctttaggac ggaatcgaat gacttattat
     1621 gtgtcgaaac ttctcgttac gcttttgatc acaacacttg gcgtggtttt gattacgggg
     1681 ctagccatga ttgcttacac acttgctttc ggatttggtg aagttgaggg aatcaaaaac
     1741 tatggaaatt atgttctaaa agtatttcct gtattgcttc tgctgatatt agcgacatta
     1801 tctgttccag tggctttgac attcatcact agaagtacca gtgtcagttt gcttctttct
     1861 tttctttata tcatgggaac ggccttcgtt cccggtgttt tcgcaaaaat caaaggcttg
     1921 gaatttctaa cggagtggtt tacggaaacg tggctcatgt atacggactt tgcgcaacaa
     1981 gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc tagtaacaat cgtggtatca
     2041 tcagctctag gaatgtttat tttccaaaaa tctgacatta agtaggtgtt cctgctggaa
     2101 actattttga ttatcgaaga cgatgaagct gttcattctt tactggagga ggttcttgaa
     2161 cagagataca aacttcttga tgcttattca ggtacggaag gaaaattatt gttaagcaca
     2221 tatcctgtcg atttgatcct tctagatctc atgcttccag ggctttcagg tgaagcattg
     2281 ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg ttctttcagc aaaaagcgat
     2341 caaagagata aagtttcctt gttggcagca ggagcggatg actatgtgac gaaaccattt
     2401 gatatcgaag aactgttgtt acgaatcggc atccaacttc gtcatcagac ttctgtccaa
     2461 gtaaaagatc tgtcgcaacg catctattac aaggaaatct tactggacaa agagtcgagg
     2521 gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa gagaattcga tctgctgaag
     2581 ctttttttag aacatccgaa aaaagtattt tcgcgagcaa atctttatga aacagtttgg
     2641 caagaaccat attttgatag tgagaaaaca gtgaatatgc atatttccaa tcttcgaggg
     2701 aaactagcaa aggcacatgc tgtgtatatt catacggtgt ggggagtggg gtttagattt
     2761 gattaagtgg ctgttgcttc tcgttgttat ctttatggga agttttattt tcttcctgca
     2821 aagggaaatc aagagattaa ctaagaaaat cgccactctt tctgaacgac aagaatatgg
     2881 tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg gacctagtag atgcactgaa
     2941 tacatgggtg gatgaaaatg aacaaaaagg acaagcattt caagaaatgg aagaaaacat
     3001 cagactatcg atcgtcggcc tttctcatga tctcagaact cctttgactg ctattaacgg
     3061 ttatgtacaa ttactggaac aggtagagga tacggagaaa cgggagaagt atttaacgat
     3121 catcaaacaa tctgttgcaa gattactcag tatgacggat cagttttatg atcttgctcg
     3181 aatagagacg aagcaaaaag aaatggatct ccatacgctt tctttttcta aagaggtgga
     3241 agaagtattt ttctcttttt atgaacaatt ttctgagaaa aatatcgagg tagcgttcgc
     3301 tgaacactcg ataaatacaa ctgtattagc agatccgctc atgttgctgc gagtgctgca
     3361 aaatatcata cagaatttat tgcgttatgc agatggacaa gcaaaggtga cattttcaaa
     3421 agaggattca ttcattcgat taacagtggc caatcctatc aaaccaggag cgcaaacctc
     3481 tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc agccgcacaa atacggaatc
     3541 tagcggaatc gggctttacc tgtcaaaaaa attggtggaa ggaatgggag gagagatgac
     3601 cgctagatta gacgggggca tcttctccat ctccgtcaag ttgcggaggg tttaattggc
     3661 tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg ttttaaagac atcttctttt
     3721 ttggaaggtg tttttatttt ggtgtgattg atacgaatta ggtaacgaag acagttattt
     3781 ttgtaccccc ttttcaaata gcgcaacagt gctatactaa aactaacaaa tcaaggaacg
     3841 gagagggata tgggccaaag agaatttatc gttttaatta ttattgcagt tgttatttta
     3901 ttagttgttt tagacatttt ttcacgtttg aaagtaaagg aaacagttcg tagtaattgg
     3961 ggaaaaattc cttatcaacc gagatttgat aaagaagaaa gtttgaagga tgcgtggctc
     4021 acggagaaaa aattccgttc atgggatagt gaaatcgacg atcttacttg gtatgacttg
     4081 gatatgttcg aagtatttga agggatcaac agtacctatt ctagtgtagg ttcagaagcg
     4141 ttgtatcagc gtttacgtag ctttgatttt ggagaagatt atcagttaga aaaactaatt
     4201 gctttttatc aggaaaatcc tcaactgaga gaaagaatcc aatatcaatt cgctcgttta
     4261 ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg atggaaaaag taaaaagatt
     4321 ggacgcgtag gtttatttat ttttcttggc ttgcttccgc tggtgggtgt aggtttgctt
     4381 cttttagggc aagtactagg aatctatcta gcaatcggtg gcttagtatt caatacggtc
     4441 tattatctag tgaagaaaca aacactagaa acagagctga atagcatgag ttatttggtt
     4501 tcaacgattt cagccgccaa tcagttagca aaaatgacga caccacttca aaaagagctg
     4561 aaacagcaaa ctaaaccatt aagagacatc cctaaattag gtttttcatt tcgaatgaag
     4621 agcgggagtg aagcagaatt gatttttgaa tatgtcaaca tcatgttcat gctgccattt
     4681 atctcctatc atttcgttct cactaaatta gaaaagcatt caactgaagc catcaagctg
     4741 tgggaattgc tgggagaatt ggaagttgca gcagccgttt tgaattatcg cacctatatg
     4801 ccgatcactt gccgaccaga gtttactgag ggcggtgtga cagcgaaaga tatctatcat
     4861 ccattgttaa aagaagcggt agttaatcca gtcaactggc agaagaatac attagtcacg
     4921 ggttccaatg catcaggaaa atctacgtat gtcaaaagta tagccatcag ctgtattcta
     4981 gcacagacga tccaaacagc gattgcagaa cagtttaccc tgcaacctgg acatgtgttg
     5041 acttccatgg caatcgaaga tgatttattt gaaggcgaca gttattttgt ttcagaaatc
     5101 aaatcgatca aacgtttgct ggaccaagtg gcttccaaag aacgctgcta ctgcttcgta
     5161 gatgagattt taaaaggaac aaatacgatc gaacggattg cttcttccgc ttcagtcgtt
     5221 cggtggcttg cggagtatcc atctttagca tttgtcgcca cacatgatat cgaattgaca
     5281 gagattttaa agaattattg tgagaatgtc cactttgaag aacaagtaac agaaggaaaa
     5341 ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa ggacccgaaa tgcgattgcc
     5401 ttgctgaaag ttttgaatta tcctaaatcc atagttgaac aggcccaaaa agaaagtttg
     5461 ttgttcgatg aacagcgtca gtggtatccg tttgattaac tttatcaaac gaagcatgat
     5521 gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg atgccaaaaa agtggcagaa
     5581 ttcaatggga acgagtctgc tgtccagatg atcttggaag atttagcaaa agacgataca
     5641 aaataaacca cgctgtcttc cttctagcag cttttatcga aaaaacttag catcagcgac
     5701 gagacgcacg ttccatggca aagttctccg taaaacaaac agcgaaaaca gaaattgaaa
     5761 ataactattt ccattttatt tatttcggtt aaaataaaag aaaaaagctt tcgataaaag
     5821 gacggaggaa agcttaacat gaataaaaaa gacaagaacc tcagttataa ggatcttcta
     5881 tatgtcatcg tagtagttgc tgtcggtact tttatgattt taaaagttcc agagttgttt
     5941 agatagttat tctatcgaag atgatcatga actagaaaac acatctcctc tttgttagca
     6001 ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg ctgtattcca tgatacaatt
     6061 aagaatcatg tctataagga gataggaaat aaatggcaaa tgcgcttgaa ataaataatt
     6121 tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg aatcgatctt tcggtgaaag
     6181 aaggagattt ctatgcgcta ttagggccaa atggagctgg gaagtctaca acgatcggga
     6241 ttattacttc tttagtcaat aaaacatctg gaaaagtgaa tgtcttcggt tatgatctgg
     6301 acaaggatct agttcgagca aagcaacaga ttggattggt tccacaagag ttcaatttta
     6361 acccttttga aacggtccag caaatcgtcg tgaatcaagc aggctactac ggtgtctctc
     6421 gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca gtccaatctt tgggaaaaga
     6481 gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg aagactgatg attgctaggg
     6541 cattgatgca tgaaccacgt ttgctgatct tggatgaacc aacagcagga gttgacatcg
     6601 agttgagacg cgagatgtgg gagtttctac gagaattgaa cgaaaatggt acaacgatca
     6661 ttttgacgac gcattatcta gaagaagctg aaatgctttg tcgcaacatc ggaatcatcc
     6721 aatcgggtga actgatcgag aatacaagta tgaaagagct tctgtcaaaa ctgcaatttg
     6781 aaacatttat ctttgattta gcgccatatg ataccaaacc tgtgattgaa ggatatgctt
     6841 gtacatttga agatgagcag acaatcgctg tcgaagttga acgaaatcaa ggggtgaacg
     6901 gaatcttcga tcagcttaca agacaaggaa tcaaagttct ttctatgagg aataagtcga
     6961 atcgtttaga agaactgttc ttgaaaatca cagaagaaaa taatcatacg gaggagaaaa
     7021 atgtttaatc tttattttac tgcattaaaa agtttagcta tcaaggaaac gaaccgctat
     7081 ttgcgtatat gggtccaaac actggttccg ccagtgatca cgacatcctt gtattttgtt
     7141 attttcggaa aaatgatcgg tgggcggatc ggtgacatgg gtggtttttc atatatggaa
     7201 ttcatcgttc ctggtttgat tatgatgtct gctatcacta gttcgtatgc caatgtttct
     7261 tcgtccttct tctctcaaaa atttcagaaa aatatcgaag aaatcttggt agcacctgtt
     7321 cctacgcatg tgattatttg gggctttgtg attggtggtg tgggtcgcag tattctcgtc
     7381 ggctctctcg taacgatcat ttccttgttc tttgtaccac ttcatgtgta ttcatggttc
     7441 atggtcatca ttacattatt gatgacggca atcttgtttt cattagcagg actgctcaac
     7501 gggatttttg ctcagtctta tgatgatgta tcgatcgtgc ctacatttgt cttgcagccg
     7561 ttaacttatc taggtggcgt tttctatgcc atctctatgt taccgccctt ttggcaagct
     7621 gtttcaaaag tcaatccgat cgtttatatg atctccggtt tccgttacgg attccttgga
     7681 acgatcgatg tgccagtagc aacatcgatg atcgtacttg tgttattcat cgttgttttg
     7741 tacgccgtta cttggtattt gatcaataaa ggaagaggat tgagaagtta
//
LOCUS       DBNIBO010000117         7774 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.121, whole genome shotgun sequence.
ACCESSION   DBNIBO010000117 DBNIBO010000000
VERSION     DBNIBO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.121"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            337..705
                     /locus_tag="AC1Y77_002159"
     CDS             337..705
                     /locus_tag="AC1Y77_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIQ1575710.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            885..1826
                     /locus_tag="AC1Y77_002160"
     CDS             885..1826
                     /locus_tag="AC1Y77_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HIQ1575711.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1895..2626
                     /locus_tag="AC1Y77_002161"
     CDS             1895..2626
                     /locus_tag="AC1Y77_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1575712.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2770..4950
                     /locus_tag="AC1Y77_002162"
     CDS             2770..4950
                     /locus_tag="AC1Y77_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIQ1575713.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4970..5452
                     /locus_tag="AC1Y77_002163"
     CDS             4970..5452
                     /locus_tag="AC1Y77_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HIQ1575714.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5468..6229
                     /locus_tag="AC1Y77_002164"
     CDS             5468..6229
                     /locus_tag="AC1Y77_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIQ1575715.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6360..7694
                     /gene="murC"
                     /locus_tag="AC1Y77_002165"
     CDS             6360..7694
                     /gene="murC"
                     /locus_tag="AC1Y77_002165"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIQ1575716.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2607 a         1350 c         1701 g         2116 t
ORIGIN      
        1 cactctgagt cacaaagagc aatttcaaaa aatagcttct catgtttgtt gaatattgtg
       61 tcttgtgacc gaggagttgt caggagaaca ccgtttacca gaggttgtga cttgttttta
      121 gtcacgacct ctttttttat ttattccata taaatacaca ttatttattc cgaaaagtga
      181 aagcgcaaat aacaaaaaca accaacttat ttattattaa agaaaagcgt atttaagtag
      241 gacgattctg ttgagagctt tcgtagattt tcccattttc ctatggtaaa ctgacaaagt
      301 tgagaatgtt agaacagatc gtttaggagg aaacagatgt ttacgaatga aataaaaatc
      361 atgctttatg tggacgatgt agaaaaaaat gcaacgtttt ggagagcagt tggctttgcg
      421 gaaaaagatc gtcaagaaat ggatgggacg cttataattg aagtaggagt tgaggacagt
      481 caaacctcat ttctgttata tgatcgagca tttgttgagt cccattcacc tggcacagct
      541 actagtgccc catctttgat gttttacagt aaagatatct ttggactata taaaaaaatg
      601 cagacgcaat cagtcacatt aggtgatatg gtacagcttg gagaagaata tgtctttaac
      661 tttgctgatc ccgatggcaa ctactttgcg gttacaggta aataatagaa ataaagactt
      721 tgtttttctg tgcgaaacag gaaagcgaag tctttttaat tcaaataata atattatgta
      781 aacaaaaaac tttccatgcg taattataca caagcaaata aatcgtcaaa tgcgaacgcc
      841 ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa atacatggat tatcaagtac
      901 tgctttatta caattataca aaaatcgaag aaccagagga atttgccaaa aatcatttag
      961 ccttttgcaa atctttggat cttaaaggtc gtattctagt agctgaagaa ggaataaatg
     1021 gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat gaaagcaatg aaagctgatc
     1081 ctcgatttgc tgatacgatg ttcaagatcg atccaagcga aggacatgcc tttaagaaga
     1141 tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct agaagaggac attgatccaa
     1201 accaagtgac aggtgcctat ctttcaccgc aagaatttaa agaagctatt ttagatgaac
     1261 agacagtggt gattgatgca cggaatgatt atgaatatga cttgggacat tttcgagggg
     1321 ctgtacgccc agatatacgc agttttcgag agcttccaga ctggatccgc gagcacaaag
     1381 aagaatttat ggaaaaacgg gtagtgacct actgtactgg tggcattcgt tgcgagaagt
     1441 tttctggttg gcttgtccgt gaaggattca aagatgtcgg acagctgcat ggcggtattg
     1501 ctacttatgg taaggatcct gaagtccaag gagaattatg ggacggcaag atgtatgtgt
     1561 ttgatgaacg gatcgctgtc gatatcaacc aagtagataa acaaatcgtc ggtcgcgact
     1621 ggtttgacgg aacaccatgt gaacgatata tcaattgtag caatcctgag tgcaatcgtc
     1681 agattcttgc ttccattgaa aatgaagaaa aacatttagg agcatgtagc atagagtgtg
     1741 cgaaacatcc gcgcaatcgc tatgttcaag accatggaat ctctccagaa gaagcagaac
     1801 tgcggtttac agctttaatt aaatagaaaa caatagtttt gattgtcgac catttccaat
     1861 atgcgctata ctagcgttga ggatggtgat ccacatgaaa tcattgattt atgctattgg
     1921 ggatgtccat ggcaaacatg agctttttga aaaacttatt cactattatg atccaaagat
     1981 ccatcagctt gttttattag gggatctcaa tgatcgaggt cctcgtacaa aagaatgttt
     2041 tttattagga aagcaattag tcgaagaaac aggagccatt tacctccggg gaaaccatga
     2101 agaatatttt ttgcaatttc tgcaatcacc ggaggactgg tatgcgcctt atatgagaaa
     2161 tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg gcatcggagg aatactcccc
     2221 aactgaaatc tcgatgatga tccaatcgag atattcagaa ctgattaaat ttttagtcca
     2281 gcgccccttg tattatgaat gggaaaatta tctttttgtc catgccggtg tggatttgac
     2341 aaagaaagac tggcgtaaga caagtctaag agactttatt tggataaggg aaccatttca
     2401 tgaagggaaa aacaatacag gtaagacaat tgtatttgga catacgatta caccaatgct
     2461 ccatggagat atgcagacaa cagacctctg gattcaggat cataaaatcg gaatggacgg
     2521 tggggctgta ttcggagggt cattgcatgg ggttgttttt gacaaggaag gtattcttca
     2581 agatattgaa tatcagaaca acgaaggacc ttggcatgga atctgactaa tggaaagaat
     2641 gaaggagctg tgactttgtt gcggctcttt ttatttttat agagaatatt gtttctttca
     2701 gtagtttaac ccaggataaa aagtatgata gaataaagaa gcaattttta gcagaaaaga
     2761 ggcattttaa tgacggaaaa tttgaaccag acgatcattc agcttgtaca aaaagaactt
     2821 tcagactatc gtcctaagca gttgactact gtacttaatt tgttaaatga aggaaacact
     2881 gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa gtcttgatga agtccagatt
     2941 cgtgaaatcg aagagcggta tgcctatctt gaaaacttag aaaaacgtaa aactgaagtg
     3001 attcggctaa tcgatgaaca agggaaactg actcccgagt tagaagcaga aatcacccaa
     3061 gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat acaaacaaaa acgacggaca
     3121 aaagcaacga ttgctaaaga aaaaggactt gaaccactcg cgaagtggct gatgcagctt
     3181 acagatggag aggtgcaatc tgaagcagaa aaatatattg acaaagaaaa agaagtctct
     3241 tctgccgaag aagcgttgca cggtgctcat gagattattg ctgaacaagt cagcgataat
     3301 gcaaaatttc ggacttggat tcggtcttat acgtataaca aaggtatgta tgtcagcaat
     3361 gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa tgtattatga ctttgcagaa
     3421 cccgtccata aaatggtttc tcatcgaata ttagctacta atcgtggaga aaaagaagat
     3481 attctgaaag tattctttca agtagatgaa gcagcaattt tagcttactt ggatcgccaa
     3541 atagtcaaaa atcctgcctc tccttcttct tcattcgtca gagaagccta tcaggatagt
     3601 tataaacgct ttattcagcc agcaatcgag cgagaactgc gcaatgaatt gactgaaaag
     3661 gcagatgagc aagcaatcgc tattttcggc gaaaacctcc gtaatctttt gttgcaaccg
     3721 ccactaaaag gtaaagttgt cctgggattt gatccggctt atcgtactgg atgtaaacta
     3781 gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag ttatttatcc tcataaacct
     3841 gcagcgcaag caaaaagaga agctgctgga ccagctttta tccaattaat taataaatat
     3901 caagtagata tggtagctat cggaaatggt acagccagtc gggaatctga actttttgtg
     3961 gctgagcagt tgaaatccgc cgaccataaa acctattacg ctatcgtcaa cgaagcaggc
     4021 gcttctgttt actccgctag cgaaattgca cgaaaagaat ttccgcatct acaagttgaa
     4081 gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc ctttagctga attagtcaaa
     4141 atcgatccta aagctgtcgg agtaggccag taccaacatg acgtttctca aaaacgtttg
     4201 gctgaacaat tggattttgt tgtggaaaca gcggttaacc aagtaggtgt ggatgtgaat
     4261 acagcaagcc cgcagctctt gcagcatata tcaggattga ataaaacaac tgcccaaaac
     4321 attgtcattt atcgtgaaga aaacggagaa ttcaccgcac gaactcaatt gaaaaaagtg
     4381 ccacgcttag gaccaaaagc atatgaacaa gcaatcggat tcttgcgagt ccctggtggg
     4441 aaaaacattt tggacaacac aggtatccat cccgaaagtt attctattgc aaaagaaata
     4501 ttaacttcag tccaactttc agaaaaagag cttggtacag aagaagcagt agaaaaattg
     4561 acacgattat ccgtagagaa actggctgaa agcctatcag tcggagaaga aacgttagcc
     4621 gatattttag ctgggctgac acaacctgga cgagatatgc gtgatgagat gccagcgccc
     4681 ttattacgta cagatgtatt gagtatggaa gacttgaaac cagggatgga attgactgga
     4741 acggtccgta atgtcattga cttcggcgca tttgttgata tcggtgtgaa gcaagacgga
     4801 cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc atcctacaga tgtcgtgtca
     4861 gtaggagata tcgtaacagt ttggatagaa caagtcgata cgaaaaaagg tagaatcagt
     4921 ttgacgatgc tttcccctta tgaagagtaa caatgaggag taacaaaaat tgacacaaga
     4981 agaactgcag cagttagtag aagagatatc attaaaaagc ttccagcttc ctttcaagca
     5041 ccaagcaatg ttcaatccac gcttacgtac aactggcggg cgctatcatt taaacgatca
     5101 tcatttagat tttaatgcga agctatttga agagtcagac gaagagacga ttgcaggaat
     5161 catcaaacat gaactttgcc attatcatct tcatctagct ggaagagggt atcagcataa
     5221 agatatggat ttcaagtatc ttttgaaaaa aacaggaggg ttgcgctatg caccagcctt
     5281 gacgaagaaa cagataaaga tggagtatta tcgatgccaa gattgttctg ccacgatctg
     5341 ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga agatgtcatg gagaaatcaa
     5401 atggattgag acaaaaagag aagactttaa tgaagcacaa aaaatcagat gaaaggatag
     5461 aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac tggaatcagc tattaaattt
     5521 tttagccgca cctgaagagg gaaattttga ggaactgacg gcacctacac ttattttagc
     5581 tggaaactgt cttcctatct tagctgataa agcagcacaa atgtatttaa atggccaagt
     5641 agatcaattg tttttggtcg gaggggtggg gcatgcgact agaatccttt atgagaattt
     5701 tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa agtgaaatct gtcgtcagta
     5761 tctaaaagaa gtctatgatt tgcccgataa ggcttttctg atagaatcga agtctacaaa
     5821 ttctggagag aacgccactt tttcactgga gattcttcat tccttaggag ctgtaccaga
     5881 aaaaatctta ttgatgaatg atccgacact gcaacgcagg actcgagcaa cttttgagaa
     5941 agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg ccatttgtgc cggaaatcct
     6001 tcacttttct gaagagatca tctttacagc aaaagaactg aatcatcaat ggcctaagga
     6061 atatttccat gcgctagtat taggagaaat ggaacgtctc catgacgatg agaacggcta
     6121 tggtccgaaa ggaaaagatt ttattcctca cattgatata tcagaggaag tttggtcttc
     6181 ttacaccaga attaaagagt caataaaaac ggatttttct cgtacttgac cattttagca
     6241 agcgaatcct cctttccaaa taaatttggt tgaaattttc aatcgtttat aagataatga
     6301 ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag ataagagaag aggattcaaa
     6361 tggaaaatca aaataaattg tatcattttg ttggtatcaa aggttcagga atgagctcct
     6421 tggctcttgt tttacatgaa caaggtctaa atgtccaagg atcagacatt gaaaaatatt
     6481 tctttacaca aagagattta gaaaaagcaa atatcacgat tctgccattt aacgcagata
     6541 atgtaaaacc agggatgacg atcattgcag gaaatgcatt tcctgattca catgaagaaa
     6601 tccagcgagc aaaagaatta ggtttggaag tgattcgcta tcacgacttt attggtcatt
     6661 ttatccaaaa ttatacgagt atcgctgtaa caggctctca cggaaaaaca agtacgactg
     6721 gactgctctc tcatgtattg agtggtgtcc gtccaacaag ttatctaatt ggagatggaa
     6781 caggacatgg tgatccgcaa gcggaattct tttcatttga ggcctgtgaa tatcgccgtc
     6841 atttccttgc ctattcacca gactatgcaa tcatgaccaa tatcgatttt gatcatccgg
     6901 attattatac aagtatcgac gatgtattta cagctttcca aacaatggct ggacaagtga
     6961 aaaaagctat ttttgcgtat ggtgacgatg cttatttacg caaattaaaa gcaaatgtgc
     7021 cgatttatta ttatggtgtc acagaaaacg atgatatcca agcacgaaac atcgaacgca
     7081 caacttctgg ttctgctttt gatgtctatc atggggatga atttgttggg cacttcactg
     7141 taccagcatt cggtaaacac aatatcttaa atgctttagg tgttattgca gtagcttatt
     7201 tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct gactttccca ggagtaaaac
     7261 gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt cgatgattat gcgcatcacc
     7321 ctgcagagat caaagcaacg attgatggcg cacgccaaaa atatccagat aaagaaataa
     7381 ttgcagtttt ccaaccccat acgtttacgc gtacgattgc attgatggat gaatttgcag
     7441 aagcacttga tttagctgat aaagtttact tatgcgatat ttttggttcg gctcgggaag
     7501 aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat caaaaaaggt ggagaagtca
     7561 tcaaagaaaa taatgtatct ccattgttag attatcatga tgcagtagta atcttcatgg
     7621 gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa acttttaagc agcacgacga
     7681 aaaatgtact gtaatacaat tactgcagtg tccttataaa aatagaaaga gggggagtgg
     7741 caaaaatctt gccaagcccc cttttttgca actt
//
LOCUS       DBNIBO010000118         7759 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.122, whole genome shotgun sequence.
ACCESSION   DBNIBO010000118 DBNIBO010000000
VERSION     DBNIBO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.122"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            145..1092
                     /locus_tag="AC1Y77_002166"
     CDS             145..1092
                     /locus_tag="AC1Y77_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575717.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1104..2024
                     /locus_tag="AC1Y77_002167"
     CDS             1104..2024
                     /locus_tag="AC1Y77_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIQ1575718.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2065..3522
                     /locus_tag="AC1Y77_002168"
     CDS             2065..3522
                     /locus_tag="AC1Y77_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIQ1575719.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3867..4481
                     /locus_tag="AC1Y77_002169"
     CDS             3867..4481
                     /locus_tag="AC1Y77_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIQ1575720.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4474..6198
                     /locus_tag="AC1Y77_002170"
     CDS             4474..6198
                     /locus_tag="AC1Y77_002170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1575721.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTLK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6195..7646
                     /locus_tag="AC1Y77_002171"
     CDS             6195..7646
                     /locus_tag="AC1Y77_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1575722.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1288 c         1543 g         2310 t
ORIGIN      
        1 gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt
       61 tcattttccc tatactttgt ttatttattc tcattattga aagcgctata attacgttgc
      121 gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg
      181 aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt
      241 tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca
      301 gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa
      361 tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta
      421 gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg
      481 agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct
      541 tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat
      601 cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg
      661 ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac
      721 tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct
      781 agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt
      841 ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt
      901 cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat
      961 ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt
     1021 gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct
     1081 gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct
     1141 ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct
     1201 gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga
     1261 ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg
     1321 accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag
     1381 gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact
     1441 ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga
     1501 tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat
     1561 tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac
     1621 gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa
     1681 tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg
     1741 ccgcattggg ttactggaat gactggttca atgctttact atacatccaa gatgataagc
     1801 tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa
     1861 attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca
     1921 gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga
     1981 agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa
     2041 atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt
     2101 tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca
     2161 gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc
     2221 gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat
     2281 atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg
     2341 gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt
     2401 gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa
     2461 gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat
     2521 gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata cgatctttca
     2581 attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa
     2641 gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat
     2701 tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc
     2761 atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac
     2821 aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga
     2881 aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg
     2941 acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca
     3001 gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca
     3061 gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg
     3121 atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat
     3181 ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat
     3241 acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca
     3301 gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt
     3361 aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca
     3421 gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg
     3481 aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag
     3541 gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca
     3601 gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt
     3661 tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag
     3721 ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt
     3781 tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta
     3841 taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc
     3901 ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga
     3961 tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat
     4021 tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa
     4081 gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat
     4141 atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg
     4201 ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga
     4261 tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt
     4321 ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat
     4381 tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt
     4441 ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat tctattttca
     4501 aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg
     4561 atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa
     4621 aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg
     4681 agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc
     4741 tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc
     4801 gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta
     4861 agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac
     4921 aaaggtgggg aaataaaaaa ggggacacta aaattactag acgcctttta tatcaagttg
     4981 cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa
     5041 ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct
     5101 ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa
     5161 agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat
     5221 ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct
     5281 gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt
     5341 ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat
     5401 tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag
     5461 acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac
     5521 gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag
     5581 caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca
     5641 ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg
     5701 tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa
     5761 gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga
     5821 gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg
     5881 atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat
     5941 gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat
     6001 ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa
     6061 gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc
     6121 ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt
     6181 acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt
     6241 tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag
     6301 cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa
     6361 cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac
     6421 gtcataattt tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag
     6481 gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg
     6541 aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag
     6601 aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg
     6661 aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg
     6721 caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact
     6781 atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg
     6841 atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg
     6901 gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc
     6961 tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag
     7021 aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat
     7081 tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga
     7141 tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga
     7201 ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg
     7261 tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt
     7321 acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta
     7381 cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca
     7441 aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga
     7501 aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca
     7561 acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt
     7621 accgagaaaa cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa
     7681 aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc
     7741 atgacctctc gataaacgg
//
LOCUS       DBNIBO010000119         7669 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.123, whole genome shotgun sequence.
ACCESSION   DBNIBO010000119 DBNIBO010000000
VERSION     DBNIBO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.123"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(19..339)
                     /locus_tag="AC1Y77_002172"
     CDS             complement(19..339)
                     /locus_tag="AC1Y77_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HIQ1575723.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(487..2721)
                     /locus_tag="AC1Y77_002173"
     CDS             complement(487..2721)
                     /locus_tag="AC1Y77_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1575724.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQQTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3200..3466
                     /locus_tag="AC1Y77_002174"
     CDS             3200..3466
                     /locus_tag="AC1Y77_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HIQ1575725.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3466..5193
                     /gene="ptsP"
                     /locus_tag="AC1Y77_002175"
     CDS             3466..5193
                     /gene="ptsP"
                     /locus_tag="AC1Y77_002175"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIQ1575726.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5357..6403
                     /locus_tag="AC1Y77_002176"
     CDS             5357..6403
                     /locus_tag="AC1Y77_002176"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIQ1575727.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6420..7643
                     /locus_tag="AC1Y77_002177"
     CDS             6420..7643
                     /locus_tag="AC1Y77_002177"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIQ1575728.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
BASE COUNT         2413 a         1383 c         1557 g         2316 t
ORIGIN      
        1 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
       61 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
      121 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
      181 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
      241 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
      301 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
      361 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
      421 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
      481 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
      541 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
      601 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
      661 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
      721 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
      781 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
      841 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
      901 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
      961 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     1021 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     1081 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     1141 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     1201 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     1261 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     1321 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     1381 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     1441 ttctttttct tttaattcac ccactggaat acctgtctgt tgttcaacga ttttttccat
     1501 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     1561 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     1621 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     1681 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     1741 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     1801 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     1861 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     1921 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     1981 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     2041 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     2101 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     2161 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     2221 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     2281 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     2341 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     2401 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     2461 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     2521 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     2581 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     2641 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     2701 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     2761 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     2821 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     2881 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     2941 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     3001 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     3061 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     3121 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     3181 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     3241 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     3301 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     3361 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     3421 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     3481 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     3541 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     3601 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     3661 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     3721 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     3781 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     3841 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     3901 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     3961 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     4021 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     4081 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     4141 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     4201 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     4261 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     4321 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     4381 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     4441 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     4501 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     4561 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     4621 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     4681 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
     4741 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
     4801 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
     4861 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
     4921 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
     4981 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
     5041 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
     5101 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatac gatggaagaa
     5161 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
     5221 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
     5281 tagacaaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat actatataag
     5341 tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga tgcttgcaaa
     5401 atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag tcggtatctc
     5461 ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat acggaatcaa
     5521 tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct atcatgcgca
     5581 ttctacagag gaagatttcc gaaattcctt tatcggatcg aaccagcttt ctcctatcgt
     5641 aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc caacacctta
     5701 ttcaaaacag ttgttggaaa gctatggtat ccgtgtgccg attcaagcta tttccaatgg
     5761 gatcgactta gagaaatatc accctgatcc aatgaaagaa caaaagttca gagaatattt
     5821 caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg aacgtaaagg
     5881 aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta tttggtttgg
     5941 tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag aagatcatcc
     6001 tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg cgtattcagg
     6061 tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg ttttagaagc
     6121 tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg gctggttaga
     6181 agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac tgatccaaca
     6241 gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag caaaagagcg
     6301 aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac tgaatgaaaa
     6361 agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag gcgaaaaaca
     6421 tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt gctacatcga
     6481 tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt tttacaacga
     6541 ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt gtaccattta
     6601 tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat ttggttgcca
     6661 aagaactaga actagatctt attcataccc atacagaatt cggagcaggg atacttggga
     6721 agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg atgtatgaag
     6781 actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta aaatacttgt
     6841 ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt gttattgata
     6901 cattacgcag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt atcgacattg
     6961 aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg gagtcactag
     7021 gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat gagaaaaata
     7081 tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac gccagattag
     7141 ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca gaactgcagt
     7201 tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca ctttattata
     7261 aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt acttatacag
     7321 aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg aaccgattgt
     7381 tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca ccagcactta
     7441 ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct aaactttatg
     7501 atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac atggttgttt
     7561 atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga atcaaaataa
     7621 aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaat
//
LOCUS       DBNIBO010000120         7633 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.124, whole genome shotgun sequence.
ACCESSION   DBNIBO010000120 DBNIBO010000000
VERSION     DBNIBO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.124"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            168..2642
                     /locus_tag="AC1Y77_002178"
     CDS             168..2642
                     /locus_tag="AC1Y77_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIQ1575729.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(2745..4481)
                     /locus_tag="AC1Y77_002179"
     CDS             complement(2745..4481)
                     /locus_tag="AC1Y77_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1575730.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4478..5194)
                     /locus_tag="AC1Y77_002180"
     CDS             complement(4478..5194)
                     /locus_tag="AC1Y77_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1575731.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELGLEL
                     ALEQSFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADD
                     YLTKPFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLI
                     ELTPKEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPK
                     HPKYLVTIRGFGYKFQEPKR"
     gene            5325..5828
                     /locus_tag="AC1Y77_002181"
     CDS             5325..5828
                     /locus_tag="AC1Y77_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HIQ1575732.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5788..6126
                     /locus_tag="AC1Y77_002182"
     CDS             5788..6126
                     /locus_tag="AC1Y77_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HIQ1575733.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6147..7565
                     /gene="ffh"
                     /locus_tag="AC1Y77_002183"
     CDS             6147..7565
                     /gene="ffh"
                     /locus_tag="AC1Y77_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIQ1575734.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
BASE COUNT         2441 a         1415 c         1620 g         2157 t
ORIGIN      
        1 cgaaaaaaag agctacgaca tagaactgtc gtagcttttt ttttgctata tgacaggaat
       61 gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta tactggatag
      121 gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg aagaataaat
      181 ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa gtcatccgtc
      241 tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga atagaaaaag
      301 gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc gtattggaaa
      361 acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt tgtttagtag
      421 aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga atcgccattt
      481 ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat acagcaaaag
      541 tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt gtaatagatg
      601 acattgttat tttagagact ggacatcaaa ttccagcaga tgcagaggtc gtggaaggga
      661 aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc aaagaaatag
      721 gcgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc cgattagaca
      781 aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca atgggagaag
      841 gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt gggatcatca
      901 ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga aacacgatca
      961 aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa ggactgtact
     1021 tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa agagttcttt
     1081 tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt gtagataaaa
     1141 caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt gatcgtctct aaaaaacaag
     1201 aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca aaagcgatgg
     1261 caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc actgatcaat
     1321 cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta gcgttggcag
     1381 acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg ccggaatatg
     1441 cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt ggtgtgtacg
     1501 atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc tatattctga
     1561 ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa aaacagcaag
     1621 tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta gctgtccaag
     1681 cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa gaagaatatg
     1741 aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa aagaaaatct
     1801 tcgttcaatt gctaaagcgt cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     1861 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     1921 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     1981 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     2041 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     2101 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     2161 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     2221 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     2281 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     2341 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     2401 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     2461 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     2521 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     2581 agcaaaagcc gtttagtcgc gtagttaaaa aatttctatc gatatttaaa cgagaaaatt
     2641 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     2701 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     2761 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accatgaagg
     2821 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgccggctt
     2881 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     2941 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     3001 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     3061 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     3121 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     3181 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     3241 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     3301 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     3361 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     3421 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     3481 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     3541 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     3601 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     3661 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     3721 tcgttcagta acgtatataa ttgctcttct gtggtagtat acgcccgata catcttactc
     3781 atttgttcgc tcaacgcatt gatcgtctga tacaattctt cccattgatc gggtgtttgc
     3841 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     3901 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     3961 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaccg
     4021 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     4081 gcgacatata aaagatcccc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     4141 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     4201 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     4261 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     4321 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     4381 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     4441 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     4501 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     4561 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     4621 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     4681 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     4741 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     4801 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     4861 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     4921 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     4981 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     5041 aatcaaatga ttgttctaat gctaattcta atcctaattc taatccttcg cctccatctg
     5101 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     5161 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     5221 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     5281 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     5341 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     5401 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     5461 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     5521 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     5581 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     5641 gcccttcatg cggaacccat tcgatttatg acgggagttt ttccggaata aaaaaaaatg
     5701 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     5761 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     5821 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     5881 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     5941 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaacttcat
     6001 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     6061 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     6121 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     6181 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     6241 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     6301 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     6361 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     6421 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     6481 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     6541 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     6601 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     6661 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     6721 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     6781 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     6841 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     6901 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     6961 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     7021 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     7081 atgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     7141 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     7201 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     7261 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     7321 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
     7381 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
     7441 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
     7501 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
     7561 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
     7621 atcagcaatt ttt
//
LOCUS       DBNIBO010000121         7602 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.125, whole genome shotgun sequence.
ACCESSION   DBNIBO010000121 DBNIBO010000000
VERSION     DBNIBO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.125"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(5..628)
                     /locus_tag="AC1Y77_002184"
     CDS             complement(5..628)
                     /locus_tag="AC1Y77_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575735.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="AC1Y77_002185"
     CDS             complement(787..2184)
                     /locus_tag="AC1Y77_002185"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1575736.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="AC1Y77_002186"
     CDS             complement(2216..3187)
                     /locus_tag="AC1Y77_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1575737.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="AC1Y77_002187"
     CDS             3561..3986
                     /locus_tag="AC1Y77_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575738.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="AC1Y77_002188"
     CDS             complement(4474..5175)
                     /locus_tag="AC1Y77_002188"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIQ1575739.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5191..5631)
                     /locus_tag="AC1Y77_002189"
     CDS             complement(5191..5631)
                     /locus_tag="AC1Y77_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIQ1575740.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="AC1Y77_002190"
     CDS             complement(5631..6206)
                     /locus_tag="AC1Y77_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HIQ1575741.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            6447..6656
                     /locus_tag="AC1Y77_002191"
     CDS             6447..6656
                     /locus_tag="AC1Y77_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HIQ1575742.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(7054..7530)
                     /locus_tag="AC1Y77_002192"
                     /pseudo
     CDS             complement(7054..7530)
                     /locus_tag="AC1Y77_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2171 a         1580 c         1213 g         2638 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg
     2041 atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt
     2281 tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga
     2341 tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg
     2401 caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca
     2461 tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt
     2521 aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct ggacaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg
     3421 gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
     4561 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     6301 ggatagacgc cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat
     6361 aaaacaaaaa taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact
     6421 gggatttcgt cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt
     6481 ttattaacga aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc
     6541 taaataaaaa agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc
     6601 tcctaaaaat ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg
     6661 tcactgctgg aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt
     6721 ttgatattgg gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact
     6781 gtataataac tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct
     6841 tcgacataag gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt
     6901 catttcaagg agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt
     6961 atattcgatt ctttctaact ctgattcttt cataccattc ctgcctcaag gtacatctat
     7021 tgtacttttt tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga
     7081 tatgtcttct tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt
     7141 caataataaa atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc
     7201 ccatggtctg gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc
     7261 gccccaaaac atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt
     7321 gcgtgccaag tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat
     7381 aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat
     7441 tttccctaag aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa
     7501 tacagtgatt ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata
     7561 aatggtaatc cagaaacagc cgtgtatctg cggtaataag cc
//
LOCUS       DBNIBO010000122         7600 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.126, whole genome shotgun sequence.
ACCESSION   DBNIBO010000122 DBNIBO010000000
VERSION     DBNIBO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.126"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(138..428)
                     /locus_tag="AC1Y77_002193"
     CDS             complement(138..428)
                     /locus_tag="AC1Y77_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575743.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="AC1Y77_002194"
     CDS             complement(546..806)
                     /locus_tag="AC1Y77_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575744.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="AC1Y77_002195"
     CDS             complement(943..1356)
                     /locus_tag="AC1Y77_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIQ1575745.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="AC1Y77_002196"
     CDS             complement(1812..2297)
                     /locus_tag="AC1Y77_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575746.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="AC1Y77_002197"
     CDS             complement(2436..2651)
                     /locus_tag="AC1Y77_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIQ1575747.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="AC1Y77_002198"
     CDS             2875..3675
                     /locus_tag="AC1Y77_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1575748.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="AC1Y77_002199"
     CDS             3694..5562
                     /locus_tag="AC1Y77_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1575749.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="AC1Y77_002200"
     CDS             5555..6046
                     /locus_tag="AC1Y77_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HIQ1575750.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="AC1Y77_002201"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="AC1Y77_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HIQ1575751.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /gene="srlE"
                     /locus_tag="AC1Y77_002202"
     CDS             6606..>7600
                     /gene="srlE"
                     /locus_tag="AC1Y77_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HIQ1575752.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1443 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DBNIBO010000123         7552 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.127, whole genome shotgun sequence.
ACCESSION   DBNIBO010000123 DBNIBO010000000
VERSION     DBNIBO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.127"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(99..623)
                     /locus_tag="AC1Y77_002203"
     CDS             complement(99..623)
                     /locus_tag="AC1Y77_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIQ1575753.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(616..1089)
                     /gene="tsaE"
                     /locus_tag="AC1Y77_002204"
     CDS             complement(616..1089)
                     /gene="tsaE"
                     /locus_tag="AC1Y77_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIQ1575754.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1212..2192)
                     /gene="pta"
                     /locus_tag="AC1Y77_002205"
     CDS             complement(1212..2192)
                     /gene="pta"
                     /locus_tag="AC1Y77_002205"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIQ1575755.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2345..3025)
                     /locus_tag="AC1Y77_002206"
     CDS             complement(2345..3025)
                     /locus_tag="AC1Y77_002206"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIQ1575756.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3151..4020
                     /locus_tag="AC1Y77_002207"
     CDS             3151..4020
                     /locus_tag="AC1Y77_002207"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1575757.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4046..4561
                     /locus_tag="AC1Y77_002208"
     CDS             4046..4561
                     /locus_tag="AC1Y77_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HIQ1575758.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4608..5171)
                     /locus_tag="AC1Y77_002209"
     CDS             complement(4608..5171)
                     /locus_tag="AC1Y77_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575759.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5226..5405)
                     /locus_tag="AC1Y77_002210"
     CDS             complement(5226..5405)
                     /locus_tag="AC1Y77_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575760.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5482..5991)
                     /locus_tag="AC1Y77_002211"
     CDS             complement(5482..5991)
                     /locus_tag="AC1Y77_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575761.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6087..6467)
                     /gene="gloA2"
                     /locus_tag="AC1Y77_002212"
     CDS             complement(6087..6467)
                     /gene="gloA2"
                     /locus_tag="AC1Y77_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HIQ1575762.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6797..7546
                     /gene="pnuC"
                     /locus_tag="AC1Y77_002213"
     CDS             6797..7546
                     /gene="pnuC"
                     /locus_tag="AC1Y77_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIQ1575763.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2143 a         1566 c         1339 g         2504 t
ORIGIN      
        1 aaaaaaatgg actgcgacac gacttttgtc acagtccctt cgtgtgtttt tctgccttct
       61 caaaaggatg acagtttctc actcttttcc tatttcttct attcaatcaa ataactcatt
      121 ttatatacat ctaaaaattc acctagatct gttcttgccc ctctaggcat gaccgcttcg
      181 atttggaagc ccatttttcg gtaaaggtgt acggctcttt cattacgaac ttgaacgtca
      241 agctccaatc gaaaaagtac acccatttcc ttcgcccaac tgatgatttc ttctagcata
      301 agagtgccta gtcccattcc ccaatattcc tgcagaatac tgataccgac ttctcccacg
      361 tgagacaaac gaaattgact gtctgccttg acagatgctg ttccaataat cgtcccttca
      421 tagattgcaa gcagcaataa attattattg ctttcgtata tataatccaa ctcttcttct
      481 aaggtttctg gtggtaacag gagttctgct tcatccaaaa ctaaaaattc tgtctcctta
      541 ttaacttgtc ccatcatcaa aagaatgtct tttgcatctc ctggcactgc ttcccgcaag
      601 atgatcccaa cttcttcact catgattttc agcccatttt tctagaattc gctgtttgaa
      661 ggtttcactt tgctccccgt atgagattag tttcacatac cgcttttcta aatctgtatc
      721 acttttttct aaaatcagtt ccaaataatc ctccggcagt gcttcttcta atagatttcc
      781 ccattcaaca acggaaagcc catcaccttc aaaatattca tctaacccta aatctgctcc
      841 gctagctgct acccgataaa tatccatatg atacaacgga atccgacctt gactatattc
      901 tctaataatc gtgtatgtcg gacttttgat catttgcgtt atccctaatc cttgggcaat
      961 tcctttcgtc aacgttgttt tacccgcacc tagatcacca gtcaatatca gattgtcacc
     1021 tggcattgcg gaatgaccaa ttgcctctgc gaattgttct gtcattgtta atcctgatag
     1081 ttcgatcatt tttcatcttc tcccgctctt ctttcttttt gccagtatat cgcgtaatga
     1141 agttttttta aagaaaaaat gaactagtca agaacagcag ctgctgtttt tggctagttc
     1201 gcgtgtattt gttaatcaat caatgtttgt gctgctgtga taatcgataa tttatataca
     1261 tcttcttcat ttgctccacg agacaaatca gaaaccggtt tgttcaagcc ttgtagaatt
     1321 ggtccgattg cttcaaagtt accgaaacgc tgagcaattt tatagccgat atttcctgac
     1381 tgcaattctg gaaatacaaa gactgttgct tgaccagcta cttctgaatc tggcgctttc
     1441 agttgtgcaa ctgaaggaac ataagaagca tcaaattgca attcgccgtc gatcatgtat
     1501 tgtggcgcta gttttttagc gattttagtt gcttcaacta ctttgtctac ttctggcgat
     1561 ttcgctgagc cttttgtcga gaagctcatc attgctactt ttggttcgat atcgaacaat
     1621 tctgctgttt ttgcagaatc tacggcgatt tcagccaatt cttgcgcagt tggattcacg
     1681 ttgatcgcac aatccgaaaa gacatatttt tcttgatctc ttccacgtac catgatcatc
     1741 gctccgcttg tccgactgat gccaggcttt gttttgatga tctgaagtgc tgggcgaaca
     1801 gtgtcgccag tcgaatggat cgctccagag accattccgt ctgcaatccc catatacgta
     1861 agcatcgtac caaagtaatt cacatctttt aaaatctttt ctgcttgttc tttggatgcc
     1921 tttccatttc tacgttcaac aaaagtatcc accatttcat cccacttatc gtaattatca
     1981 gggctgatga tcgtaaagct agatgttttg attcctctgg catgcgctgc atttcttatt
     2041 tcttcttcgt tccctactaa aatgggttcc acaagttctt ctgctttcaa ccgggcagct
     2101 gcgcctaaaa tacgagcatc agtagcttcc gggaaaacga ttttgatccc acggcgaaca
     2161 attttaaatc ttaaactatc aaacaattcc acaagatgac ctccagacgt ttttatatgt
     2221 attatcatac actattccag aaaaaaagaa cagtttattt tatctgtatc agtcaaaaat
     2281 gctggaaaaa ttaagttttt cacatacaaa gcgaactgca ctataacagg aaagcgctgt
     2341 tttattacac tgtttcaggc agttgccaat caatcggttc ttctcctaac gcaaggagcg
     2401 catcattcgt tttcgagaag ggtttggaac caaaaaagcc acgacttgca gataacggac
     2461 tgggatgtgg agatgttatg atgatatggc gactcttatc gatcattttg attttttctt
     2521 gtgccggttt tccccacaag ataaacacga ttggtttttc ccgttcattt aatttttcaa
     2581 tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg agaatacgct tgcccttccc
     2641 gaacggtaag aacggtgttg agtaaaagga ctccctgttt agcccatgac actagatttc
     2701 catgttgaac aggtgcgatt ccaagatcat cataaagctc tttatagata tttcttaacg
     2761 atggcgggat ctttacgcca ggttgcacag aaaaagataa gccatgagct tgattttctc
     2821 catgataagg atcttgtcct aaaatcacca ctttaacttc ttcataagga gtcaattcca
     2881 atgcttcata gatatgatac atatctggat ggatcttttg ggacgcatat tcttttttta
     2941 aaaattctct tagatgcaga taataatctt ttgaaaattc tgaggacaaa acttcttgcc
     3001 aactgttatg aatgatttct ttcaaaaact ccacctccta ataaaaagaa taactcacat
     3061 tttcttcaat cacaagggac aatcttcacc ctcagcactt gattaatatg gtaaactagg
     3121 aacaagaagc agaaaggaac gataatcatt atgatcaaat taattgcatc agatatggac
     3181 ggcacattat tagatgccca tatgagtgtt tctcctgaaa atgccgaggc gatacgtttt
     3241 gcaaatgatg ccggtgttga gttcatggtc gctactggtc gaaactatca agaagctcgc
     3301 gcagctttag atgaggtagg aatcgattgt gcgatgatca ctttgaacgg ggcacaggta
     3361 tttgataaag aaggaaattc attgtttacc gtgtcgattc caaataatca agcaatctct
     3421 gttttagata ttctagatgc taacggtatt tactatgaag tagcaaccaa cgatggtctt
     3481 tattctgaaa atcaagcgaa acgaattgaa agttttgctt ccatggtggc tacccattta
     3541 cctcacctta cgtataaaat ggcgattgcc atggcctcag ctaagctgga gttattgcac
     3601 atcacatacg tggacagtat tcgtgctatt ttggaagaaa agaatctgga agttttaaaa
     3661 attatttgtt ttcatacaga agggccagct gtattaggtc cagttggaaa gcagatttct
     3721 cttttaggcg atctagccgt tacttcctct ggtcaaaaca atatcgaagt gaaccataaa
     3781 aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg agcgtgggat ttctttagaa
     3841 gaagtcatga cgattggcga taacttcaat gatgtcagca tgctgcaagt agcaggagtc
     3901 agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt atgccaaata tacaacggac
     3961 acaaaccttg aatctggcgt aggaaaagcc atattgcgcg cgattgcaga agacttgtag
     4021 ttttatctag aaaagaggga tatagatgtc agaatttttc attcaggaaa agcagcttgg
     4081 acgtgcaact cggacaattg tcaaaaacga aaaaggacat tccctgtttt tgatggttgg
     4141 tcgctgggga acaaagggcg atgcattgtc tctttatgcc atgaatggcg atctcgttgc
     4201 tcacatcaag cagataagcc tgacattcgg tacgcgtttt gagctgtata aagagttcga
     4261 aaaggtggga accatgcaga aaatattcaa ttggcccggt gacttttatt atattcgtag
     4321 acttcactgg acagtccaag gtgatatcta taatcatcaa tatcaaatcc atcatttcaa
     4381 tcagcaaatc atgaaaatgg ataaagcaac acttttttca ggagattact atgtattgga
     4441 tgtctcaaat gatgaagatg ctccagtttg tatttgtatt gctgcggttt tagattactg
     4501 gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct taccgactta acttaagtta
     4561 aaaagatgta cctgaaatgc ggaaatacgc atttcaggta catcttttta gatattcttt
     4621 tgcatatgat cataaagatg accactgatc atccgctgac cgacaacttt gaagtcatgg
     4681 cgttcgtata attttttagc ctttggattt ttttcatcca cacttagacc gatgacggag
     4741 cgattagctt tcttcgccaa tttaggtaat gcttctaata atcgcgaacc gatgccttgt
     4801 cctctaaaat cttctgatac actgatagag tccaaatacc attcgttggg aaacgtttct
     4861 ttatcaatga acaaacgtgt agacgcatct agctgatatt tctctaagat aggtaccagt
     4921 ggctgatcga tgattgattc ttcttcatct gtataaccgt aagcaactcc tgcaacttta
     4981 ccgtctattt cttctacgat acctcgtgaa taactgtaac gataatttgg atcagtgatt
     5041 gcttcttcta aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa tggcagttcc
     5101 atatctttta gaatcactaa gatcaatggg gcgatttctg gagcatcttc ttttctcgca
     5161 aaacgaatca tagtttcctc tccttttctc cattctaaca tgcagaaaaa gaaatcgcaa
     5221 aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg agtaacgatt
     5281 cttgccgctt cagttcggca ataagggcag acgactggca tttctttatc attatgttcg
     5341 actacattat tgacttcttc gtatgcaaat ttttttccgc aattagggca tttcattttt
     5401 tccatgttat ctcaacttcc tttcatcttt actctcttag cttactctta tattgtaaaa
     5461 aagagaagca atatccttct tctatgcaga tatccatacc tgacggtcct gttctccatc
     5521 tattaatcgg actttataat aaggacgttc atctttgact agaagtgtcc actctttcac
     5581 ttttgctttt ttcccgtagt agctgactgc ttccttcatc gctgcttgcg gttgtttgaa
     5641 ctcttccgtt ttttctatat caaaaagatg ttctgcggcc ttttccgtgt catttcgtac
     5701 ggttgttttc ccagtggatg gattgactgc tatgacttgt ccttcacaaa aaaactggta
     5761 ttcatatgtt gcattcgtag gattctccaa atctttttca actgcaaaat gaatagcaga
     5821 tatgttatgt tcttttgttt cgttttttac gattgctgct gcttttttca atgagatccg
     5881 atattcctgg tcactaatat catccatgtg aaaagcggca ttgagcagtc ccgtcatacc
     5941 ggtaatccct attacgatga gggcagcaac accaaacatc atttttttca ttcttcattc
     6001 tcctgtttct tttttttaaa gaatatcaag aaatgaacga atgaaaaatc gaatgaaacg
     6061 aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt ctaatggcaa tccgtctgga
     6121 tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt ctctaatcgg ttctgattcg
     6181 atccctaagg actgcaattc tgcaattgtt tcttcaatat cgtctacttt gaaagcaaga
     6241 tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg gcgcattagc aattccaaaa
     6301 atctccaatt cagcacttcc tagtttcaga tctaatttat agtcatctcg ttctttgcgg
     6361 taattttctc tgattacttc aaatcctaat ttattaacat aaaaatcacg tgatttctga
     6421 taatctgaaa caatgatggc tatatgatgg atcgttgtaa ggttcatatg agcactccta
     6481 ttcttcgttt aagctcggat ctatattaat ctccagttcc attgtaaatg tgtcagattg
     6541 aaaaaaatag ctgtttttcc tttttataag aaaaaaggca aaaaaaacag aaagtaggtc
     6601 ctccaagaca ctcttcctgt tcccaatata ataaatgaaa gtaagaaatt atattttcaa
     6661 gaaaacaaac agaaaattat cataacacaa attatttata attgaaagac tagttttttt
     6721 tcaagaaaag catacaatag attgtgttag taaatacaga acacactaca tttagtgtct
     6781 taaggagtcc tcatacatga agtcatattt tcagttttta gctcatcagc taaaaggctg
     6841 gccgcaacaa aattattatc ttttcttttt cagtcttggc tgccaggtca tgactcttgt
     6901 caatagtccc atcacttggc tgtccgttat tacatttatc ggtaccacgc ttggtgtatt
     6961 gtgtatttta tccatcaatg cggcaaaatc agtcaacggt attttaggta ttttgtctgc
     7021 gatttgtttt attatcgttg ggttttcagc aaagaactat ttaagtatcg gtgaacagat
     7081 tgcttatatg atcacattgg atatccccgt attgttaagt aaagaatgga atcataatat
     7141 ggcttcaaaa atccgaaaat tcactggaaa aagctggttt atcgcaatca tcagtacttt
     7201 ccttgtctat cttgtttcag gattcctgat tggtcgttat acagatgatc ctcggccgtg
     7261 ggtagatgcc attgcttttt ctattagctt gactgctggt atcatcagtt tcttgcgtta
     7321 caacaaccaa tattactggt ggttagcttc agggcttgct caaatggtcc tttggtatat
     7381 ttcatttacg catggatctg caagtttggc gatgttcgtg aacagctctg tttacttgct
     7441 gaatgatgtc cttgcattta ccgtttcacc ttggtataac caaaaagaac gtgaacgtat
     7501 gcaaaaaatg gaagaagaat actacaacgc acaacaacac gcataaaaaa ac
//
LOCUS       DBNIBO010000124         7550 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.128, whole genome shotgun sequence.
ACCESSION   DBNIBO010000124 DBNIBO010000000
VERSION     DBNIBO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.128"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(131..697)
                     /locus_tag="AC1Y77_002214"
     CDS             complement(131..697)
                     /locus_tag="AC1Y77_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1575764.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="AC1Y77_002215"
     CDS             complement(697..1653)
                     /locus_tag="AC1Y77_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HIQ1575765.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="AC1Y77_002216"
     CDS             complement(1653..2309)
                     /locus_tag="AC1Y77_002216"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIQ1575766.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2679..4697
                     /locus_tag="AC1Y77_002217"
     CDS             2679..4697
                     /locus_tag="AC1Y77_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIQ1575767.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4767..4973
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5124..7538
                     /gene="leuS"
                     /locus_tag="AC1Y77_002218"
     CDS             5124..7538
                     /gene="leuS"
                     /locus_tag="AC1Y77_002218"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIQ1575768.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         2505 a         1453 c         1545 g         2047 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtagc cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
     2881 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     2941 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3001 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3061 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3121 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3181 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3241 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3301 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3361 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3421 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3481 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3541 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3601 cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca aacggaggac
     3661 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3721 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3781 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3841 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3901 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     3961 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4021 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4081 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4141 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4201 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4261 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4321 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4381 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4441 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4501 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4561 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4621 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4681 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4741 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4801 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4861 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4921 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     4981 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5041 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5101 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5161 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5221 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5281 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5341 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5401 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5461 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5521 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5581 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5641 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5701 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5761 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5821 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5881 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     5941 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6001 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6061 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6121 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6181 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6241 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6301 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6361 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6421 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6481 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6541 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6601 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6661 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6721 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6781 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6841 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6901 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     6961 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7021 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7081 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7141 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7201 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7261 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7321 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7381 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7441 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7501 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt
//
LOCUS       DBNIBO010000125         7510 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.129, whole genome shotgun sequence.
ACCESSION   DBNIBO010000125 DBNIBO010000000
VERSION     DBNIBO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.129"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(2..2167)
                     /locus_tag="AC1Y77_002219"
     CDS             complement(2..2167)
                     /locus_tag="AC1Y77_002219"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HIQ1575769.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2427..4721
                     /locus_tag="AC1Y77_002220"
     CDS             2427..4721
                     /locus_tag="AC1Y77_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIQ1575770.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4714..5379
                     /gene="pgmB"
                     /locus_tag="AC1Y77_002221"
     CDS             4714..5379
                     /gene="pgmB"
                     /locus_tag="AC1Y77_002221"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HIQ1575771.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5372..6379
                     /locus_tag="AC1Y77_002222"
     CDS             5372..6379
                     /locus_tag="AC1Y77_002222"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIQ1575772.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6401..7420
                     /locus_tag="AC1Y77_002223"
     CDS             6401..7420
                     /locus_tag="AC1Y77_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1575773.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2340 a         1639 c         1410 g         2121 t
ORIGIN      
        1 cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca
       61 ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcgt
      121 tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt
      181 ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt cccatatgga
      241 tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta gggaaaatac
      301 ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc
      361 cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt
      421 ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta ctacctgctt
      481 gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat
      541 cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt
      601 ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt
      661 ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt
      721 cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc
      781 tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag
      841 caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga
      901 tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata gctaacggtg
      961 tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca
     1021 tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca
     1081 taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa
     1141 aatacattga tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag
     1201 ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg
     1261 tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta
     1321 aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg
     1381 cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca
     1441 tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta
     1501 gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg attccagcct
     1561 gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa
     1621 caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat
     1681 tatagtactt attataagcc attgccccga caaatccagc gatgatccct acgaatactc
     1741 ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg
     1801 taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa
     1861 agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca aatgctccgc
     1921 ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac
     1981 caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat
     2041 caggattgat caagggaatt gatttcccta tactgatcat caacccagct gccggcataa
     2101 ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct
     2161 ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt
     2221 tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct ataaaaccta
     2281 taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta agcttttttc
     2341 tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta
     2401 taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc
     2461 catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa
     2521 caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg
     2581 accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct
     2641 ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg
     2701 cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact
     2761 tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt
     2821 ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag
     2881 cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa
     2941 aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga
     3001 ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact
     3061 ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag
     3121 tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct gcagaattat
     3181 ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc
     3241 cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata
     3301 tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg
     3361 tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct
     3421 ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa
     3481 aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt
     3541 tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag
     3601 caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttca
     3661 ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg
     3721 cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa
     3781 atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt gtccattttt
     3841 caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca
     3901 atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag
     3961 aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa gaactagcca
     4021 aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg
     4081 tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg
     4141 atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc
     4201 aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaagc
     4261 aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta
     4321 tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc
     4381 gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca
     4441 cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc
     4501 atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata
     4561 tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcgagt
     4621 tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc
     4681 gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta
     4741 gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag
     4801 gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt cagccgagaa
     4861 gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc aaaggaagaa
     4921 tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa
     4981 ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc
     5041 aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa aatgggtctt
     5101 acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca
     5161 gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc
     5221 gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg
     5281 ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg
     5341 atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct ccgtcgtacc
     5401 agttccagag actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct
     5461 tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga
     5521 aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg
     5581 agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt
     5641 ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt
     5701 ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga
     5761 tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa
     5821 cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa
     5881 ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac
     5941 ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac
     6001 aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc
     6061 atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac
     6121 ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa cttctaataa
     6181 aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca atggaaaaca
     6241 gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt
     6301 ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta
     6361 tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga
     6421 tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc
     6481 tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt
     6541 tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt
     6601 accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc
     6661 aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt
     6721 tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat
     6781 tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa accacatccc
     6841 tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga
     6901 taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat
     6961 cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca
     7021 aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga
     7081 ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat
     7141 tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct atgtcattcc
     7201 agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta
     7261 ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg
     7321 cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat
     7381 caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaatac
     7441 gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact tctaacacat
     7501 tgtttatctg
//
LOCUS       DBNIBO010000126         7443 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.130, whole genome shotgun sequence.
ACCESSION   DBNIBO010000126 DBNIBO010000000
VERSION     DBNIBO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.130"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(58..1434)
                     /locus_tag="AC1Y77_002224"
     CDS             complement(58..1434)
                     /locus_tag="AC1Y77_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIQ1575774.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1562..2680)
                     /locus_tag="AC1Y77_002225"
     CDS             complement(1562..2680)
                     /locus_tag="AC1Y77_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1575775.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2822..3256
                     /locus_tag="AC1Y77_002226"
     CDS             2822..3256
                     /locus_tag="AC1Y77_002226"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575776.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3334..3867)
                     /gene="ntdP"
                     /locus_tag="AC1Y77_002227"
     CDS             complement(3334..3867)
                     /gene="ntdP"
                     /locus_tag="AC1Y77_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017110 - nucleoside diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside tri-diphosphate phosphatase"
                     /protein_id="HIQ1575777.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3961..5139)
                     /gene="mutY"
                     /locus_tag="AC1Y77_002228"
     CDS             complement(3961..5139)
                     /gene="mutY"
                     /locus_tag="AC1Y77_002228"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIQ1575778.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5132..5929)
                     /gene="recX"
                     /locus_tag="AC1Y77_002229"
     CDS             complement(5132..5929)
                     /gene="recX"
                     /locus_tag="AC1Y77_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIQ1575779.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            6036..7418
                     /gene="rlmD"
                     /locus_tag="AC1Y77_002230"
     CDS             6036..7418
                     /gene="rlmD"
                     /locus_tag="AC1Y77_002230"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIQ1575780.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
BASE COUNT         2231 a         1519 c         1302 g         2391 t
ORIGIN      
        1 gaaaagacgt gttgcttgtt tattcaagca acacgtcttt cataaccaag tttccaatta
       61 tcttcttggc tcgtcatctt cgaattcttt tctgaagtat cttcgttctt cttcaggttc
      121 cgcttctgtt tcttccggat catgaaaaat gacacggatt ttaaggattc gagaaccttc
      181 catttcttca gaaaccagcg taatattatc gacatcaaac gataactttt cgccttcatc
      241 cggaatcatg cctaatgctg taatcaaata accagccatc gtatctacat cactcatatg
      301 cagatcactg tcaaaagctt cattgaactc atcaatcagc atacgtcctt ggatgatata
      361 ttcgtgttca tcgatttttt cataaagatt ctcaacttca tctgattcat catcgatttc
      421 tccgacgatt tcttctaaaa ggtcttctag tgttgctaac ccaacgacgc cgccatattc
      481 atctaataaa atagccatct gattctgcgt ctttttcatt tcatataaca gatcatcaat
      541 aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt ttcttgatat ctaaattatc
      601 aaagccgaat ttatgggcag cttttagtaa attctttgta tgaaggattc cgactacttt
      661 atctttgtct tcgttataaa cagggattct tgaatagttt tcggataaaa cttcattcac
      721 attttcttcc acagtgtcgt tgatatcaat cataaatgca tccgttcgtg ggaccatgac
      781 ttctcgggca actttcgtat caagagaaaa aaccccttgg agcatttcta attcttcatt
      841 attcaatacg ccttcgtttt caagcatata acgcatttca tctctggtca tttttgaatc
      901 ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa cttgtggaag cagaaagcag
      961 ccagacgaag ggcttagcta ctacaccaat caagcgtatc agccctgaag tgaactttgc
     1021 gacttcttct gatttattta aagcaatacg tttagggtac aattctccaa aaacaatcga
     1081 tacataggtc aaaatagcca gcacaatgat atttgcaata ttttttgccg ctgtgccgcc
     1141 acctaatacc ggagctaacc gttccgataa agtattagct agtgaagcac cagacaaaat
     1201 gttgaccaaa gtgattccta cttggattgt cgataggaaa ttatttggat tctgcaatat
     1261 ttttaaaagc ttttgagatt tcacatctcc ttcatcagct ttttgctcga ttcgattctt
     1321 attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac
     1381 gatcaataat aaaatctgcg ctaacagcga ctgactctca gggtcagcat tcataatcgc
     1441 attctctcct tataaaatta atttttagtt tacgatagaa aaaaacgtgg cactatagcc
     1501 acgttaaaat ggtatcacaa agcaattttc tttgcaataa aaacaagtgt actaaaacta
     1561 cctacgaggt cgtagcaatt ttcttcttgt tttcattttg cttgtcttct ttgacgattt
     1621 tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac
     1681 caagcaatcc ggcaatattt ccagctacta gaagaacgat aatgatcgtt aatggatgga
     1741 ttttcagtga tttcccaatt acattaggat aaatgatatt cccatccaac tgttggacga
     1801 ttagcacaac cacacaacaa agtaatgctt ttaccggatc attgaagact gttactagaa
     1861 atgcaggtgc tagtcctaga taaggtccta aatatggaat taggtttgtc aatcccgcaa
     1921 tgaccccaaa taagaaagca taatccacac caattaccat atatcctaaa aatgtgaagg
     1981 taccaacgaa taaacattcg attgcttgtc cgctaatgta attagccaat gttttattca
     2041 gttgccccaa taactccaca atttggtttc ttcttttttc tgggaaaaac tgttggatgt
     2101 ttggcactag tttttctccg tctttcaaca tatagaacaa gatgaatggt gctgtaatga
     2161 ttactacggt tgccgaagcg atcgttccaa cgatcgaacc taaactattg gatactccac
     2221 taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga
     2281 tatctacttc cttgaacaaa ggcaattcgg ctacctcttt taaccagctt tctacctgtt
     2341 tgatgaaatc aggcatatta gatgccaatg aggaaatttg ctgtactaga cttgggatga
     2401 cactaagtaa aatccaaata attgctgcaa ctaaaaggag cagcacaatt gcgacagccc
     2461 atattctttt gatttttgct ttcatcaaca gcttgacaat aggattcaat aaatagtaaa
     2521 gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga
     2581 aaataaagtt tatttctgat gaaatcatga tcaatgcagc tatgatcaat aattcaagcg
     2641 accaaaacat tagtttagac tgtctaagtt tttcaaacaa tagatttcct cctttctttc
     2701 ttactagtat atattatatt cgttaaaaat tcttttattc tttttttatt tttgaaaact
     2761 tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag gtggctagac
     2821 catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg ctgtacggat
     2881 caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga
     2941 tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt
     3001 acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga ttctttctga
     3061 atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact ttgctaagaa
     3121 tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt tttacgaaaa
     3181 actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc atatttatat
     3241 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
     3301 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
     3361 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
     3421 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt aattcatcat
     3481 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
     3541 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
     3601 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
     3661 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
     3721 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
     3781 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
     3841 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
     3901 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
     3961 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
     4021 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
     4081 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
     4141 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
     4201 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
     4261 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
     4321 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
     4381 cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg gctttgcttt
     4441 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
     4501 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
     4561 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
     4621 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
     4681 tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga tacttgcaat
     4741 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
     4801 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
     4861 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
     4921 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
     4981 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
     5041 ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt gaaattcttt
     5101 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
     5161 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
     5221 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
     5281 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct atcgcttgtt
     5341 ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt ctcaataatt
     5401 ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
     5461 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
     5521 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
     5581 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
     5641 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
     5701 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
     5761 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
     5821 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
     5881 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
     5941 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
     6001 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
     6061 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
     6121 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
     6181 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
     6241 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
     6301 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
     6361 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
     6421 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
     6481 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
     6541 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
     6601 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
     6661 tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
     6721 aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
     6781 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
     6841 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
     6901 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
     6961 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
     7021 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
     7081 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
     7141 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
     7201 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
     7261 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
     7321 gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag actgcacgtt
     7381 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
     7441 ctg
//
LOCUS       DBNIBO010000127         7431 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.131, whole genome shotgun sequence.
ACCESSION   DBNIBO010000127 DBNIBO010000000
VERSION     DBNIBO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.131"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="AC1Y77_002231"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="AC1Y77_002231"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIQ1575781.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3182)
                     /locus_tag="AC1Y77_002232"
     CDS             complement(1929..3182)
                     /locus_tag="AC1Y77_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIQ1575782.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="AC1Y77_002233"
     CDS             complement(3188..4111)
                     /locus_tag="AC1Y77_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIQ1575783.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="AC1Y77_002234"
     CDS             complement(4332..6824)
                     /locus_tag="AC1Y77_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIQ1575784.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="AC1Y77_002235"
     CDS             complement(6831..7298)
                     /locus_tag="AC1Y77_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HIQ1575785.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2041 a         1622 c         1188 g         2580 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat c
//
LOCUS       DBNIBO010000128         7303 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.132, whole genome shotgun sequence.
ACCESSION   DBNIBO010000128 DBNIBO010000000
VERSION     DBNIBO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.132"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(35..2134)
                     /locus_tag="AC1Y77_002236"
     CDS             complement(35..2134)
                     /locus_tag="AC1Y77_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HIQ1575786.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(2435..3259)
                     /locus_tag="AC1Y77_002237"
     CDS             complement(2435..3259)
                     /locus_tag="AC1Y77_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIQ1575787.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(3256..4011)
                     /locus_tag="AC1Y77_002238"
     CDS             complement(3256..4011)
                     /locus_tag="AC1Y77_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1575788.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(4024..6000)
                     /locus_tag="AC1Y77_002239"
     CDS             complement(4024..6000)
                     /locus_tag="AC1Y77_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIQ1575789.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(6053..6724)
                     /locus_tag="AC1Y77_002240"
     CDS             complement(6053..6724)
                     /locus_tag="AC1Y77_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HIQ1575790.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(6784..6993)
                     /locus_tag="AC1Y77_002241"
     CDS             complement(6784..6993)
                     /locus_tag="AC1Y77_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575791.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(7060..7299)
                     /locus_tag="AC1Y77_002242"
     CDS             complement(7060..7299)
                     /locus_tag="AC1Y77_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575792.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         2011 a         1497 c         1101 g         2694 t
ORIGIN      
        1 aaaacaaggc tgattctcag ccttgttttt atatttattt gagttttaaa actacatagc
       61 ccaatgatgc taaagtaaga aataatatac ttccaataac agttagcact aaatgattcg
      121 atccagtttt tggcacaatg atctgaggta agttaggttt taccccaatc gttactacat
      181 tagttgcttt ctcaatactt tctactgaaa cttttgcttg gttgtcgata tccaattctt
      241 cattcaaagg agttttagct gtaaatatca gctgaatttc tcgtccatct aaaagatttt
      301 tacttaagaa atcagcggaa gcttcccact tcagcgttct tgaattgcta tcataggtta
      361 gtgtaccttc tgaagtaata tcttttcctg tactcatatc tatcactcgc gcagaacctt
      421 ctttatacgt caaacgatct tccactggat cttgaatact aaattgcttg taatgatctg
      481 aggcaatgtc tacacctttt gtatgccatt tttgtgtgac tagatattgt acttcagatc
      541 ctgcatttaa aggagctgta ggcgttcctt ttttaccatc atttcgacca tcaacatctt
      601 tcccatcttg ttccactttt ttatttggtt taagagagaa atagttttct actgtatttg
      661 ttttttgtgt tcctttgttc ggatcattgt taaacgttac agtagccaca tttggtgcaa
      721 ttccttttgc atcatttttt gctctcatat ggagctgcaa aatataatcc ctaccgtaat
      781 ttgcagcgtt agctaaaaag ctgtcagaag cagtataagt gactgtatta cctgatacag
      841 ttaactttcc tcctgtatca gcttttttcc aagtaccatt cgcttcaaca taccacaatg
      901 cgccatcata gtttttccca tccgatacca tatcaaaatt tttgctgtca aaggtatcag
      961 tgattttaaa tgatttgtat ttgttcattg tgttgactgt taatgcatca aacttttggt
     1021 gaatatgata gtaaacatct gatccttgtg gaatcacttt tttgtcaata ttgtgtgctg
     1081 cctcttcagt attagctttg ttaaacactt cttttgtagg cttcgatggt tgtggtgtcc
     1141 atagcaatgc agtatcaaag ctaaacatca tactattcgt atttgcgcca atcatgaatg
     1201 ttggggaagc gccttgaagc gtatacgtta caccacttct gtgataagta tcagctccta
     1261 caacatcggt gaaatgatca ttcgagctat aagccttttc tgcacgtcct cccactactt
     1321 ttgaagttaa attgtttgtt gataacgcag gagctttgta ttctgcacat gctgtatctg
     1381 ttgtgacaaa ataaggtaag ctacccattt tttcataccc aacaaattct ctcatatcaa
     1441 tattacgttc accgacagat tcattattgt cattatacca agacacacgt tgctgatact
     1501 gtccatttaa actgtaaatg gatagaaatg tatcgtttaa atcaatacca gctgctctca
     1561 tcgatctcat ttttgattga atttctggct gtggttcact caaagctact tttgtttttg
     1621 ttcctgcata cttaaattct agttttgcat tcatgacatt gattgactgc atgaaaaatc
     1681 ctcgataaat atttttatca aagagcaaat aattataata ctttttgcca ttaattgttt
     1741 tagctaatcc gtcttggtta accccagcgt gcgctacaaa attagaaaaa gtcacgtcac
     1801 aatcaatttc ttttcctaga taagtaccta ctttttgata atgggcaacg atttttgacg
     1861 tacttttcaa atctttgact tttggccaag caagtacttt ttgtttttta gttgaatact
     1921 catctagtat cttaaatcca ttgatcccag ttacttcgat atttctcaat gaatcactat
     1981 catgataaag caccagatta ttattttctt tgatttgact cggatcaact ttttctaagg
     2041 cctggactgc agttcccctc gcaaaaagaa gaaggaacaa aaacaacact aaagtaatta
     2101 ctcttcccgt agtgcaatga attttatttt ccataatgcc ctccatctaa tattttttta
     2161 aacgatcgta agcttttcat tctttttttc ctaataccca cgttaccaat cctgtaagaa
     2221 gaaccaaact aaccataaaa attggattga tcaagagctt tttaatcacc tctaatggca
     2281 tggaagcaca ttgaatgttt agaaaatgaa tgaacaatcg ttgatatctt ggataaattg
     2341 ataattgaga aaaaattaaa ccgcaacaca atattgttgc ggctaaaaag gatagattaa
     2401 aaactaatat tcttattatt ttcatcattt catcctaatt atcaaaaaat aattgagcaa
     2461 aaaattccca acaattttca aaaaagttct tctttacacc agttgacaca acttttttaa
     2521 tttcttcttt cggctctgat ttctctgatt tagggtataa ccaaacatcc gtcaaaaatt
     2581 cacctttttc atttgaaaca ggtgtcaata aaacaatatc ttcactgttt gaaatgtttt
     2641 cagggcttga ttcattaacg atatagtatg cttgtttttt gtttgcaggt acttcaatgg
     2701 aaaattcaat cacgccttcc tgatcatcaa tcgttttggt ttctccttca gccacttttt
     2761 tcaactggtc gtatgaaagt ttttttgctc gatctttcaa ttttgactca attatttcta
     2821 cagattgatt tttctcatta tcatcttgtg acatttcttc taagatattc gtgatatcgt
     2881 agacaataaa cttcgcgcca ttgatacctt cttgctgggc tacttgactg ccttgattgt
     2941 tgatttgatt cgaagaaata attttttgaa tatgaatggc ttgcttgatc acttgcgtat
     3001 cttctacgtc ttgcggcttt tctgaagtct ctgaagaact ttctgacgtt ggattcgttg
     3061 attcagtaaa gcttatattt gaatctgccg tttcttctgt agtattctcc gaactgtccg
     3121 tttcgcttgg tgcagtaatt gaattatcta ttgtttgagt ggtactacta tcactgctgt
     3181 cagcgaaaga cagttgcgaa taagctacag tagaaaagag agctgctaca caaacaaaca
     3241 gccattttct cttattcatt ccgatctcct ccttattttt ttataaataa aaataatagg
     3301 aacgcaaaaa aaagcgaata gcagtgctaa tcgccaatcc aatgaaattg atttcctatt
     3361 ttttgctttt ttctgtagtt ttaaatcttc tttactgaag gaaactcgtt caccatacac
     3421 taaaaagcgg tgtgagttga caccttttgg tgtgcatgtc attaacgtga tcctatcttg
     3481 gtctttttca ggcataagat aatctaaata atttttttgt aattcttctg ctttaacttc
     3541 ttcaattcga taaatttgat aggcatgttg ttcttcattt accttaataa agaatttttg
     3601 cccttttttt actgaaggca aatcatcgaa aagattatct ttataaagtc cactgtgccc
     3661 cgcgattaaa ggatttttac cgtttcctcc tgtaatatcg ccagttcctt ccgtaatccc
     3721 tacaccattc tctaaaattt tgtcggaagt tccgtcataa attggtagtt ttaaagaaat
     3781 acttggaatt gtaagaacac caacaggatc gcctaactct attttcgcaa catccaaaga
     3841 agaatctgtt ttttcaagat tttcaatggc taaaattctt tgctgttgtt gaaatttcgc
     3901 tacatttgtt tccacaactg gaattaaata gactgctact gccgacagca accaaaaaaa
     3961 tatcatcagt ttgtgcatga ctcttctttt tgggggctta cgaataatca ttagctgatc
     4021 cttctaggct ttcttcttaa agtaaacaac tgcagaaagt ccccctgctc ctagcaacaa
     4081 gcaagagatc aatacgtaag attggaaacc gttgctacca gtcattggta cggaagcttt
     4141 ttgattattt ttgatcgtga ttcggttttc ttctttgtat gaatctttgt ctactttgaa
     4201 agagatctct tttgttaatt tttgatagtt ttcaggagct tttgtttctt ccagtgagta
     4261 atctccttca gttaaaccag tgatttcaaa atgaccctct ttatctgatt ttaggattgc
     4321 tgcatttgct acatcgtcta cccaaactgc ttctttgaca ccatctttat ctgtatcagt
     4381 aagtccaccg tagtacttac cttctgcatt cttagctgca aattctgcgc cttcaagcgg
     4441 tgttgctttg atacctgttc caaataggcc gctatcgact tttaagaatt ttgcgccata
     4501 agtcacaacg tcagctttcg ttttttcgtg gtgttcttcc tgattaaatg gattattgtt
     4561 ataaacgaaa tccacgctat ttgttaatgg atttgcgatt tcagcactat cattcacaaa
     4621 catgttataa gtcattttta gatcttgacc atgtaaattc gcaactgtat cacttacttg
     4681 tccattattt acgataaaat cgatcacaaa accactatca gaattttcga ttttatagtc
     4741 agtattttct tttaatacga ctgaaccatc attatttgtt actttaatat ctgctttttc
     4801 agcaacataa gaaagtcctt tacctgctgt atctccccat ttaaatacac tgtaaggaga
     4861 agtttcgctt ttcacaccgt ttactgtgat accgtctgcg ccacctgcaa tgtctgttgg
     4921 aatatgaatc gttccttgat aatttactgc gtcaccaatt tggaaagaat gttcttgtcc
     4981 tgttccattt gttactgttt tatcgatcac tggtgcttta tagtttacgt atgaagcttt
     5041 taagtcactt gtcacgccat cattcgtaat ttcaaaggtt aatttattgt gcgcatcgtt
     5101 gcgagcgtct gcgcctaata aatattgatc tgctgttggt tcttgatctg atcccactac
     5161 aacttccgaa ggaacttcca caaaatagta ttttcctaac gtcaaatcag tcgcagtcag
     5221 ctttccttga tcatccgata caagatcacc caattttgtt ccttttccag gttcgccctt
     5281 gtataactca aattttgccc cttttaaaag ggtttgtttg ctttggtctg cgccatcgtc
     5341 accaaatttc gttaattcaa aactcaactt agaaacaatg ttcttaggat acaaatgaat
     5401 attttttaaa aatcctgatc ctgttttatc tgtcattggc gcaatcacaa ccattggttt
     5461 tgctttttgt gtgactaatc cagcagcgga tttactttca taaaccacat atacattccc
     5521 tttggaattg acataggctg gttgatcagt aaaagtgatc tcaccatttt catctacagc
     5581 tgaagtaatt ttgttggttg cattcttgac gtactcactg ttagatccct tgtcttctac
     5641 atcttgtaca atctcgtcca ctttcgcgtt ggttagatca gaatcttctc tcggtaatac
     5701 ttgatccgta atatttccta acgtaaactc aacatcacca taatctgcct tgttgtattt
     5761 cgttacacct gtaggatatt gatcgtgcgt ataaccatcg tttttgatcc cgtcaatatc
     5821 aacattcaat tgcgttcctt gatcgtacat taatttatga accgtgatat tgaccttctc
     5881 tggtggtgtt ttttgcgtga catcttctgc atgtacttga tacccctgaa agcagcctgc
     5941 aatgattcct aacattccaa caagcattcc agcttttttt acataccgat ttattgccat
     6001 tttttactct cctcatttca tatttcaacg actcgcgttg attaaaacct tgttattgta
     6061 gctctccctg taccacgtat ctttcttttc cttgtgacgt ataaacacaa gtaaacaacg
     6121 ttaataaggg tttctctcct ttctttggca aagacaagaa agagttctct tcaggaacgc
     6181 catcgattaa tttaaagtac ccaggtacga taaatttacg cgttaccttg taacgataga
     6241 cgccatcgtt actaactaag tccatcacct cccctaatga caacttttga atatcagaaa
     6301 ataaaacacc tggcatttcc atgttatgac ctgctaggac atagtttcct ttgcccattt
     6361 cggcatcttc ataaaaataa gtagccgctc caagactcaa agtgtattga tttgcccctt
     6421 taaaaattgg caatgctaaa ccaagtgcag gaatctgaat ttgtgcaaat acttgtttat
     6481 tgacgcccat ttcgattgct tctttttgta tggacaacat ttgccccaaa gacatatcat
     6541 tattgacgtt cgttgcatcc tccttataac tctttgcatg tgcattgatt tgatctacgg
     6601 gcatcttttt ttctatattt tgcttaaaca cctctccttc atgtttttgg tagctaacat
     6661 agccaaacac actgcaaaag acgattaaag tgactcctaa aaaaccaaat aaccattttt
     6721 tcatccatct gcctcctttc agacaaaaca aaaaaacttt ttctcatttc catagaaaaa
     6781 gttttaaaaa tgaattctat aagttcgtct caactgttca aagtttttca ttgaacctaa
     6841 caaacaagct ttctcttttc ctgttaactg catatcattg atatattgcg cgacagataa
     6901 tcttttaagg tgtttttgct tcatcgtttt tagtaacttt tcttccattt ctttcgacac
     6961 tttttttctg ttttcgattt cttcataatt catgagtttt cctaccttcc ttgttttttg
     7021 caaaaataaa aaagcctatg atcacatacg ctctacactt cagtcttggg ttatcttttt
     7081 ctttcgttta attactggcc gtgctggttt caaatccttg attccattat gggttctcca
     7141 ataatcgaat tcttttcgca atctttcttt ttccatatct gttcctttag ataaggcttt
     7201 gaatacttct ttaggtgaat aaccacgagc taacagctgt acataccttc cttttgtcaa
     7261 agtatccata tccatcgcct ctaaaactgg tcgcgccatc tac
//
LOCUS       DBNIBO010000129         7249 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.133, whole genome shotgun sequence.
ACCESSION   DBNIBO010000129 DBNIBO010000000
VERSION     DBNIBO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.133"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            99..746
                     /locus_tag="AC1Y77_002243"
     CDS             99..746
                     /locus_tag="AC1Y77_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIQ1575793.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="AC1Y77_002244"
     CDS             1139..3784
                     /locus_tag="AC1Y77_002244"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIQ1575794.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="AC1Y77_002245"
     CDS             4094..5407
                     /locus_tag="AC1Y77_002245"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIQ1575795.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="AC1Y77_002246"
     CDS             5397..6050
                     /locus_tag="AC1Y77_002246"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575796.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="AC1Y77_002247"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="AC1Y77_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIQ1575797.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="AC1Y77_002248"
     CDS             6947..7147
                     /locus_tag="AC1Y77_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1575798.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2395 a         1229 c         1593 g         2032 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcagg
//
LOCUS       DBNIBO010000130         7193 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.134, whole genome shotgun sequence.
ACCESSION   DBNIBO010000130 DBNIBO010000000
VERSION     DBNIBO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.134"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            232..1107
                     /locus_tag="AC1Y77_002249"
     CDS             232..1107
                     /locus_tag="AC1Y77_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIQ1575799.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1211..1975
                     /locus_tag="AC1Y77_002250"
     CDS             1211..1975
                     /locus_tag="AC1Y77_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HIQ1575800.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1962..3404
                     /locus_tag="AC1Y77_002251"
     CDS             1962..3404
                     /locus_tag="AC1Y77_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1575801.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLTGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3415..4745
                     /locus_tag="AC1Y77_002252"
                     /pseudo
     CDS             3415..4745
                     /locus_tag="AC1Y77_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4758..5216
                     /locus_tag="AC1Y77_002253"
     CDS             4758..5216
                     /locus_tag="AC1Y77_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HIQ1575802.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            5508..6809
                     /locus_tag="AC1Y77_002254"
     CDS             5508..6809
                     /locus_tag="AC1Y77_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1575803.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            6836..7096
                     /locus_tag="AC1Y77_002255"
     CDS             6836..7096
                     /locus_tag="AC1Y77_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIQ1575804.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
BASE COUNT         2315 a         1050 c         1497 g         2331 t
ORIGIN      
        1 ttatagagag ttgctatatt ttttttgttt ttatgtaatt acctccaaat caattcgata
       61 caagtattat cataaaacga gtaaaaaaga tattgataac gcttttatta tgtgttaaac
      121 tatttttgta aattatgtag ggtgctacta ttcaattagg cacgacctaa gaaagggttc
      181 tttcttaggt cgttttttgt ctttaaaaat tgtttggaga caaaaatatg tatgaaggag
      241 tctgcaagtt gttttgtttt atcatatagt ggaggtgaat taacggtgta tgtgatgaaa
      301 aagctgaatc ataatgctgt attgatcgat gacgatggaa tagaaaaaat tgcgatgggg
      361 aaagggattg gcttttctgc taaagtgagt aagccatttg atgttgagga tgctgacaaa
      421 ttatttgtat tagattctaa agaaaagatc aaacgattca gtgagatagt ctcgcagatt
      481 ccaatcgaat ttatggattt tgcggaagaa gtgattgaac tgataacagc taggatctcc
      541 tctccattag attcaaatat ctatattaca ttgacagatc atatttattt tgctattcaa
      601 cgaaaaagag aagatagaga tgtcacagca attatgttgc cagaaatgag attcctttat
      661 ccaaatgaat atgatatagc aatagaagtg gtcgagcgaa tcaatcatcg atatcaaacc
      721 gagttagggt caaatgaagt aggattcatc actatgcata tcattaatgc tgagctagga
      781 gagcctaata gtttaaagag cttaaaaata gtagaaatga cgaccacgat tttgtcccat
      841 ctagaaaaat cctattcaaa cgtttatgat aaagaatcac tgacttatca tcgcttaatg
      901 gttcatatca aattcctggt taaacgcttg atttatcagg aagaaacgcc agtagaaaat
      961 attggttttt ttactgaaac atttaaacat agcaaagcgt attgtacagc actaaagatt
     1021 aaagatatca tcgaacagca atttcaactt gccattcaag aaaatgaaat catctatttg
     1081 gcaattcatc tagcacgaat ctattaaaat aagggcgtaa ctatctatag gcgcaaccaa
     1141 atgacacata gtaaatggac tatgattatt tggttgcgcc tattttagta gaaaaaagaa
     1201 ggagaatgaa atgggaaaag tgatttttaa ttcggatgat tttggctata gccatggtgt
     1261 gaattatggg attatggatg cttatcagcg aggaattttg acatcaacta cattgatggc
     1321 aaatatgccg ggtttcgagc acgctgtgaa actaagaaag gaaatgccta ggctaggtgt
     1381 aggagtccat ttaacattga cgtgtggcaa accacttctc aaaactgtcg atactcttat
     1441 cgaaagcgga gagttcagat ccttatctta ttatcagcat ccatttaaca ttgacaaaga
     1501 tcaactgtat caggaatgga atgctcagat tcaaaaaatt tatcatgcgg ggatcatacc
     1561 aactcattta gacagtcatc atcatactca tactttggat gaaaaccagg aggtagttgt
     1621 tacattagca aaaaaatatg atttgccggt tcggagcaac tttgaacgaa aagatgaagt
     1681 tcgtcatgtc gattattttg agccttgttt cgatgtcgta ggagaaactg atgaaaaaaa
     1741 acgacaaatc aatacgagtg tggaagaata tttggcaagt ttactcatga tattgagaga
     1801 agataaaacg atcgaagtca tgtgtcacgc agcgtatttg gatcaggaat tgttaaatgg
     1861 ttcaagtttt gtgtatcctc gtactcacca agtcgaattt ttaattcatt cagcatttgc
     1921 tcaaaaagtg aaaaatgatc ctttgattga gttagtaaca tatgaggata tttaatagag
     1981 gaggaataaa aatgaatgtt aaatcaaatg tgcagaagct tggaagagcg atgctacttc
     2041 cagttgcagc gatgccatta acgggattga tcatgcgttt atcagctgat gatatgctga
     2101 acatcccagt aattggtgca gctggaaatg ccgtttttgg aaacttggat atcttatttg
     2161 ctataggagt tgcaatcggt tttgtcaaaa ataaggacaa aggaatacct gcacttactg
     2221 gttttttagc gattgctacc ttgaaaaaag gcttagaaat catggaccca gaagtgaata
     2281 tggggatttt tggtggaatt atttccggtt tagtagcggc atggacgtat aatcgcttta
     2341 aagaacaaaa attaccagta gttttttctt attttgcggg cgaaaaattc ccattgacta
     2401 tggtgatgat tctacaaaca atcgcagcta tttttttggg ctttatctgg ccgctcatcc
     2461 aaattgggat tgataatttt gcaaaactgc tcgttgatat gggggcatta ggtgtgggga
     2521 tatttatgtt tttgaatcgt ttgctgatcc cctttggact gcaccatgtg ttgaatacgt
     2581 atatttatta cgatttagga agctatacat ctcctagcaa ggaagttttc agaggagaga
     2641 tgacacgctt tatcaacgga gatcctcagg ctggactgtt tttatcaggt ttttttgtag
     2701 taatgatgtt tggtatacca gctatatgtc tagctatcta taaagcagct tataaagaaa
     2761 ataaagagat ggtcaaagga atcatgggaa gcggtgcagg aacttcattt attgcaaata
     2821 tcactgagcc tgttgaattt agtttcatgt ttctttctcc tatgttgtac gtgatccatg
     2881 cactttttgc cggtttagca gggattgtct gttacttatt caatatacga attggtttta
     2941 cattcggagc atgtattgtt gattatctca tcaattttcg aatcgctacc aatgctattt
     3001 tgattcttcc aatcggaatt ttcttttttg ccttgtatta cgttactttt tattatttga
     3061 tcaataagag aaacattcag acattaggac gtgaagctaa ggcagagttt ggcaatgaag
     3121 taaccttaga agaaacagag ttagggttag caagtaaaaa ttatgaatat atggcaacaa
     3181 aaatgctaca agcgtttgga ggaaaagcca atatcctgga tgtctatagt tgtaatacac
     3241 gcctacgggt agaggtagtg gatcccacca tggtcgagga acagcgaatc aagcaattag
     3301 ggataagcgg gatcattaaa ccaactgaaa aaaattatca aattatcatc ggcttagaag
     3361 tgacttatgt tatggcagaa ttcaataaat tattagaaga atagaaagga aaagatgatg
     3421 atgaaaaaac agattatcac tatagctgga ggcgggagta cgtatacacc aggaatcgtt
     3481 caagccattt tgaacaatga aaaacggttg ccattatctg aaatacgttt atatgatatt
     3541 gatgaagaaa gaaatatgga tatgtacttg attgttaaat ttatgctgaa aaaaaaggtt
     3601 tttcaaatat tcgcattcgt gcaacggatg atccaaagct tgcttttaca ggatgcgatt
     3661 ttgttttctc acaaattcgt gttggaggat tggagatgcg agaaaaggac gaaaagattc
     3721 cacttaaaca tggattagta ggacaggaaa cgtgtggact cggaggattt gcttatggga
     3781 tgcgctcaat caaaggttta ttggaaattg tcgatcatat acaagactat gcccccaaag
     3841 catggatttt gaattataca aatccagagt cgatcgtttc tgaagctgtt cgtcgtaaat
     3901 atccgaatgt aaaaatgatt aatgcctgtg atatgactat ttcaattgaa gaaacgattg
     3961 cagtgaatta cggttatgat cgtaagaatt ggatcgttac gtattatggt ttaaaccact
     4021 ttggctggta tacatcgatt tatgataaag aattgcaacg agaaattatg ccagagatta
     4081 ttgaaaaact aataacaaaa gaaatgcaag tcgccgattt taatatagga gacaaaactt
     4141 ggcaaaaaac ctttcaaatg atgtcagtca tcacgaaaaa ttttccaagt aatatcccga
     4201 ataattactt agagtattac ttatatcctg atatggtcgt tgagcatact gacaaagagt
     4261 atacacgtgc taatatggtc atggatggac gagagaaaaa taccaaagaa atggctgata
     4321 aaatacgccg cggtattgtg gaagaagtat tgaactttaa ttttggggag catggacaat
     4381 atatcgtcga tatagctatt tccttgttga atgatgatcg tcgtcgcttt atgctaatcg
     4441 ttcctaatca aggggcgatt ccgaatctac ggtcagatgc agttgttgaa ataccggcat
     4501 atgttggtgc aactggtgtg gagacaatta cactaagaaa accaatcagt gattttcata
     4561 aaggattgat ggaggcacaa gttgcagcag agaagttact agtagatgct tactttgaag
     4621 gttcttatca aaaagcttta caagcattta cattgaatca aaccgtacca aatgccagag
     4681 tcgcaaaaaa aatactagat gaaatgatca aagtaaacaa aaaatattgg ccagcattaa
     4741 agtagaggtg ataaaaagtg catttcttta aaacaagaaa cattctaaaa gctgtagcta
     4801 gtggtcaaat tattccatta acagcagtaa aagacgaggt cttttcttca aaaatgatag
     4861 gagttggata cgctataacg aatcatgatg gatatatcca tgcgccagtt aaaggaataa
     4921 tcgataatat atttcctacc cgacatgcca taacgatcga aagtgaatct ggcagaaaaa
     4981 tactgatcca tatgggttta gatacagttg aactcgaagg aagtccgttt cagatacggg
     5041 ttgaaagtaa ccaggaggta aaagaaggga cactgttagc tattatagat ttgaagaagt
     5101 tggagcaagc acaaaaagat acggtgatca ttgtagtttc tccaggcatt tttgaaggaa
     5161 atttattaaa acaaaaccaa gaagtaaaga ttaaagatag ttgttttgaa ttttagtgta
     5221 cgatgaaaaa agttaaatag ggtgatattt atgggtgaat ttacaaaatt tttttgaaat
     5281 gataagtttc aatcactaga tttaatcatt ttgatttttg ttaattatcg aatttttttg
     5341 ttgctcctat gatttagaaa acttattttc aggattctta aaaataaatt tgattattta
     5401 agagaatata ttgaaataaa atttcttaat tatataatta gttatgaaca ttctttgcgt
     5461 tgtcatgcaa tgaatggttc atattttatt gaaaaggagt ggttttagtg gaaaagctga
     5521 tgaaatgggt cgaagagaaa cttgtcccac caatggcaaa aattggtaca cagcgtcatt
     5581 tattagcaat tcgtaatggg gttgtttcaa cattgtcatt gattttgatt gggacatttt
     5641 ttatggtctt tatcaattta ccgtttcctg gatggaatga atttattgcc ccttattcgg
     5701 caacgattgt tttacctttt aggatgacga tgggattgat ggctatttat gcaacttttg
     5761 ttatgggttc ggatctagct aaatcatatg gattagatag tgtgacaggt ggtatattat
     5821 ctttaggaac gttctttatg ttacaagttc ctgtaaacgt tttaactcct gaaaaggcac
     5881 ctcttggctg ggtcttacct atgagttctt tgggagcatc tgggatgttt gcggggatct
     5941 tatctatgat ttttgcagtg gaagtatatc gtttctttaa aaatcgaaat attactatca
     6001 aaatgccaga acaagtgcca cctgctgttg cccgtagttt tgaggcattg attccaggtg
     6061 cagtcatttt aacaattact tggctgattc gaagcgtcgc gggctttgat gtaaacgcta
     6121 ccttattatc gttattcgag cctttaacca atattttagg gaataatttg cttggtgttt
     6181 tactaccaat gtttttgatc catttgttgt ggtcatttgg tatccatggc atgagtattg
     6241 taggggctgt tgttcgtcca atgtggctga tcatgttgga tgagaatgca aaggcattgg
     6301 cagatggtac agctgccacc caattaccct atatcgctcc tgaacagttt tatcaatgga
     6361 cggtaacaat tggtggtgca ggagcaacga tcgtggtatc tgttttgttt atattcttct
     6421 gtaaatcaaa attcttgaaa gaggttggtc gtttttctat tattcctggt atcttcaata
     6481 tcaatgagcc catgattttt ggagctccta tgatgttgaa tccttacatg ttcatcccgt
     6541 tcaacttagt tcctttagtt ttgactatta tcagttattt tgcagtcaaa ttagaaatgg
     6601 tcaatagatt tactgttttg ccaacttgga cattaccagc tccaatcggc ggttatcttt
     6661 cagcaggaaa tgactggcgt gttgttatct tgatcatgat caacacattg atagcattta
     6721 ttatctatta tccatttgta aaagcttatg ataaaaagat gctgtcagat gaacaaagtc
     6781 gtggagaaaa cgaagtccaa actgtctaag tttggttttt agaaggtggg aaacaatgta
     6841 ttggctgatt ttattttttg tttttatctt tttattaacg gctagtcacc ttatattgaa
     6901 tatgctagct acatatcata ttcaaatcaa tcgatggatt tgggcactgg catcattttt
     6961 gatcgtgatt cttccaaaga tcattgtacc gcatatgaat gttttgtttt catgggggac
     7021 atatgtactt tgtggaattt ttgctatcaa ttttatgatc gagcagcata gatggtttgt
     7081 cacatcgaag ctttaaatgt tctaaaagtg gttgtgacag aagtggttag cttcaagaaa
     7141 taagaagaaa tttacgaaaa ttgcttctta aatttttgtg aaatgttggc tta
//
LOCUS       DBNIBO010000131         6642 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.135, whole genome shotgun sequence.
ACCESSION   DBNIBO010000131 DBNIBO010000000
VERSION     DBNIBO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.135"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..1118)
                     /locus_tag="AC1Y77_002256"
     CDS             complement(<1..1118)
                     /locus_tag="AC1Y77_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575805.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1351..1473
                     /locus_tag="AC1Y77_002257"
     CDS             1351..1473
                     /locus_tag="AC1Y77_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575806.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1589..2896
                     /locus_tag="AC1Y77_002258"
     CDS             1589..2896
                     /locus_tag="AC1Y77_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIQ1575807.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3015..4214
                     /locus_tag="AC1Y77_002259"
     CDS             3015..4214
                     /locus_tag="AC1Y77_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIQ1575808.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4241..5509)
                     /locus_tag="AC1Y77_002260"
     CDS             complement(4241..5509)
                     /locus_tag="AC1Y77_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HIQ1575809.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5564..5630)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(5672..6318)
                     /locus_tag="AC1Y77_002261"
                     /pseudo
     CDS             complement(5672..6318)
                     /locus_tag="AC1Y77_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         2016 a         1383 c         1192 g         2051 t
ORIGIN      
        1 taataacgaa gaatcgctgt cagactcttt tcatctggcg catctttttg atccaaaata
       61 aacactttcc cgccattaat gatcacatca tctgctaatg cattcaacac ttgacgccga
      121 tcatattctg tttcaggatc ttcaccgaaa ccatcagcta agtttgacgt agcgataaat
      181 aaatgtgaca cttttccttc tttcgcagct ggaacgatat caactaactg atcgatgaat
      241 ttcttatcta agagtttatt gtattctttt gattcttgag ctgcgagttc acttgacagc
      301 ttcttcgccc cttcttcgat atctctaaag gatagctgcg caggagaagc agaaatcgca
      361 acgtctgcac aataataggg atttttagca atttttttga acatcgtctg attttctggt
      421 aatgcaaata aataaatcgg ccattgttca ggattttcga aagaatcctt caagtacgta
      481 tcaacagctt ggtaatagtt gacccaatcg atctctactt cttcatcttt tggatttacg
      541 ccatggaaag ttactccatc tcgggctccg cctcctccat tagaagagaa gttcaaaccg
      601 ccacctgtca gttcagatcc caatgcagta acgacatcaa ccggtgcccc ttctggtaga
      661 gatacttctt ttacttgttt gttgctgacc tcatacaatt tcatggagtc gcggttcaat
      721 cccaataaat aataagaata gttgaactgc ccatttttga taatagccag taaataagga
      781 agatcactta catagtattg atcgtctacc gggatactca aacgatggat ataggtctgg
      841 tcagcagtta taatgaccgc tacactcgta gttgcactac gccaaaacga aggatctcct
      901 aacaaatttt caattttttc ttggaacggg gaccagtcat gttcggaata ttttttctca
      961 aaacgtttct ttgcttcttt cgcaaaattt ttgaatttga tctgatcttt ttccacatct
     1021 tgatgggcga catgcgtatt cagcatgatc gtgacgaatg gtcctttagc gccttctgaa
     1081 gtcaattgtg tcaataattc tttatccttt tttgccataa acgtacccct cctaaaaaaa
     1141 tcattccttt gtttctatct aaagtctacc acaacactat ttttccatac aataaaacgt
     1201 atctaaaaaa ataaaacggt ttctacggtt ttaaatttag ctattttttt aagttatctt
     1261 cataatttcc tcaattattt agccccagtc ttcaactatt cttcatgaaa tgctgataaa
     1321 ctaagaaaaa gaataaaaga ggtggatctt atgcatttga ttatgatttt cagtttatta
     1381 agtattatca gtttatttgc tttcagcgct tcccgcttat tagatgcgga aacacagcct
     1441 attcctgtaa aagtacgtaa aaacgaccgc taaacttcca taacgcgcgt tcttatccta
     1501 aagttttttc agaaacattg aaactctctc ccctctctga taaaatttta atttgtagcg
     1561 aaacaaatta attacaagga gatcggatat gtccataaaa aaaacacgtc tagtcctatt
     1621 attggctagt ctcattcttt gccttgcttc ttttctacca gctgttgctg tgcaagctga
     1681 agatacgttc aaagtaaatg caaaagcagc ttttgctgta gatgcgcaaa gcggtaagat
     1741 tctttatgat caagatggag aaaaaaccat ggggatcgct tcaatcacaa aaatcatcgg
     1801 tctttacatc gtccttgatc aagtaaaaga aggaaaactg tcatggggtg ataaagtttc
     1861 gatttcagat tatgcagaaa atttgagtat caccccagat ttatcgaatg ttcctttgca
     1921 taaagaaaac acctacactg tcaaagaatt attcgactct gcaatcatcc agtcagccaa
     1981 tgcttcaatg gtggccttgg cagaaaaaat ctctggcagc gaagctaaat tcactgaacg
     2041 aatgaaagaa caattgaaag attggggaat caaagatgca acaatcgtta atgcatcagg
     2101 attgaacaac tcttatcttg gcgaaaaccg accagaaggc actggagaaa acgacgaaaa
     2161 tcaaatgtct gctcaagatg tggccatcgt tgcccgccat ttgatcttgg atttcccaga
     2221 aatactggat gtatcaagta ctacgaccca aatgtttggc gaaaatacgc aatcaccagt
     2281 ggaaatggtc aactggaact ggatgctccc aggattcgtt aattacaagg aaggcgtgga
     2341 cggattaaaa actggtacga ctgagcttgc aggtgcttgt ttcgttggta cgatcactaa
     2401 aaacgatcaa cggatcatca ctgtcgtatt gaatgcggac ggccatgcgg aaaacccgag
     2461 tgcccgcttc aatgaaacag accgtttgat ggactactgc tatgacaatt ggtcagaaaa
     2521 agagctcgga aaagctaacg caagtattcc tagtctaaaa accattgatg taaaagacgg
     2581 aaaagaatca acagttcctg ttgtgcttaa aagccctgta aaagtatggg tacgaaacga
     2641 tatggatact ggcaaattaa cgatcacgcc aacacttgac aagaagcaag tcactgacaa
     2701 tgagatccaa gcacctatgg ataaaggtac aaaaatcggt accgctacga tcaccttggc
     2761 agacgataaa ttaggctatt tagaagacaa caatgcacca tcaacagaga tcattacgga
     2821 taaaacagtg gaaaaagcga atatattcgt gattggctgg cgacgcgtcg cagaattttt
     2881 cggcaacttg ttttaattga acaataagaa aaacatcttt ccatcggaaa gatgtttttt
     2941 tgctttttct agtttaaata acagcttttt tcatctattt tcgttatact gtataagaaa
     3001 aaggagttga atttatgaat ctaaccagag atctgatctt tttagttctc tatcttattg
     3061 gggtattctc tatcgtttat tggcgaaaac agaagaagga tccttccttg ttcttttacg
     3121 gcagtacgtg gacatttacg gtaatcttaa cttattttgt cgtgttggaa attttttatt
     3181 cgacctttta ttttttgatt ccattgttgt tctttagtat ctttgctttt tcttatttca
     3241 cagaaaaacg aaggctactg aatggtcttt tattcaatgt tttactcatc agtttcggca
     3301 tctacttgtt tgtcttgctg tatgaaacgc aaaatatttt tcttggcgga ctgatcgcct
     3361 tgatcacgat tccattgcta cttgtccttt tatttggtat ttatggattg attgtttttc
     3421 ttttttggaa cggggtcaca gttttacgta gagaatcgca ttcattagct aatcttttaa
     3481 cgttgatact agccattttt cttacactgt ttcttgtttt tgactttttc ttgttaaaat
     3541 atttgccgca atggataaat gctttgtttt tctgcgttcc cctgatcttg atctatctat
     3601 ttatcgtatt ctacaacttt ttgactgtct cattcttgta tcagttcaac cggcctcgat
     3661 acaatcaaga cttcatcgtt gtgttaggcg ctggattgat caatggagaa acagtttctc
     3721 ctcttctagc caagcgaatc aataaggcaa ttgcttttta tcgggctcaa agccgtgcta
     3781 cattaaatcc gcccatctta ttgatgtctg gcgggcaagg agcagacgaa aaagtaccag
     3841 aggccattgc gatgaaacaa tatgccatgg aacaaggaat ccctgaaaga gacattcttg
     3901 tcgaaaccaa ttcgacaacc actttggaaa acatgctcta ttcaaaggaa atcatggatc
     3961 agcaaatgaa agggccatac cgcgcgatct ttagttccaa caattaccac atttttagag
     4021 ccggtcttta tgcgcggcaa gccaaattga aagcaaatgg tattggtgca aaaacagctt
     4081 tctactactt gcctaatgcg tttttaagag aatacctcgc catcttgatg atgaacaaaa
     4141 aaagacatat gatcatttgc ggattgcttt tcgcaggctc cgcattctta tcgctgatta
     4201 ctttactttt ctaaaaaaac aaggtgtgac aaaacatatg tcacaccttg tttttttctt
     4261 tcattctctt aactattggc aaaaagagta tccctttttt gaccgctact tgctgagcag
     4321 aataaatacc gctgttgcct gaacacataa agctgatcaa gcagatcata aagaagtata
     4381 ctgccgcttg tgaaccgaac aattcgatcc ccatcacaaa gcaggcaatc ggtgtatttg
     4441 tcgcaccaga aaatactccg ataaagccca atcctgcaag aaaaggaaca gaaacatgga
     4501 gcaaaggcgc tagtgctgca ccaagcgttg caccaatttc aaacaatggt gtcacttctc
     4561 ccccttgata tcccgcgcct aaagacaaaa ctgtgaagaa taacttccca ataaaatcat
     4621 agacatgtgc ctgtccatta aatgattctt gaagtaacgg aagactcaat ccgagataac
     4681 gttgtgtttg caaaatcaat gctgctgcca caactacagc tccacctatc gtatttcgac
     4741 aaacaggatg aggaatccat tgtgcatatc gcgcttttaa aaagacgatc gaacggctga
     4801 atacccaacc aatcaaacca aaagcaatcc cagcaacaaa aactttgaag aacagcaaca
     4861 tactccattc aggaatcaca cccatttggt aatgtagatg agaaacacca tagctttctg
     4921 tcatgaaatt tgcaaacagt gcagcaaaga agctagggaa gatcgcttct gttcggactt
     4981 tcccgattgc caacacttct aatccaaata atgttccagc taaaggtgtg ccaaatacag
     5041 aactaaaacc cgcactgatt ccactgataa tcaagatttc ccgttctgat ttatccaatc
     5101 ggaacaaacg tgccgtatta tctgctaacg ctccccccat ttggactgct gttccttcac
     5161 ggccaacaga tcccccaaat aaatgggtag ttatggttcc aaacagtgta agtggaataa
     5221 gccgcaacgg gattttttct tctccaccat ttccttgatc gatcactaag ttgtttccac
     5281 gactagaaag tccgccgtga tacgcataga gataagcaaa aagcgcacca ctaactggta
     5341 gtagacaaag gagccaagga tgtgatagtc ggatatctgt cacaactgtc aggctattta
     5401 agaaaaaagc agacaagctc cccatgaata taccgagaat cgctgtgatc accagccact
     5461 tcagcatata cactaaaatg cgtactggac tcggtaattg tttttccata aaaaattcct
     5521 cctcaataaa aaaacgccta ctaaagaaag gggctgtccc cagactttag taggcgtcat
     5581 tagctatctt tcgttagcat ttaaaggcga acaccatcac ctattcactt ccattaaaag
     5641 atagcatatt tttctgtaaa aaacaataaa attatcgttc tttctttaca cgttcagcaa
     5701 attcatagaa aggagccaat ttcgttaact gttgattaca ttggtcacta tcatgacaaa
     5761 tataattccc ctttttcgta tacgtgccat cagaacctgc tttcgttgta gaaagaaaaa
     5821 gcgaaacatt cgatgttttc tgacagattg cacagattcc cttatttatc gttggtgata
     5881 aagtaccgct aatccctttt aatttttcat cttcgtaata gaccatgaat tttttctgcg
     5941 tacctggatc attccagccg atgtacgtat gttcttgcaa gtcgatttca ttccaagctg
     6001 gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg ctaggctgca
     6061 taaacggctg aacgatttgc ttcagctcct ccaatgctct ttctgcttgc gcttttgtca
     6121 gttttacgtc taaaactgtc gttaaaaatg tatcgattct ggcagttcgt gatctaatat
     6181 gtctcgaatc ttttcagctg ctagtgcctg aactgtttgt accgtttttg gatcattgac
     6241 agatgagtac gcatttaata actgttctac ttctcttatt ataaaaaaat attggtatgg
     6301 ttttattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc atactttgta
     6361 gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcctggactc ttttaaataa
     6421 gatacgtttt gcatcttatc atcaccccct ttaatgctta ttattctatg tacatgattc
     6481 attcgataaa taaaagtaaa ccgtatcttg aacagatagt caactgcaaa cagaaaaaag
     6541 gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat gaatggtgtt caaaagcttg
     6601 catctgcggt aataagccga aacttcacaa aaatttgaga ag
//
LOCUS       DBNIBO010000132         6610 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.136, whole genome shotgun sequence.
ACCESSION   DBNIBO010000132 DBNIBO010000000
VERSION     DBNIBO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.136"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            810..1484
                     /locus_tag="AC1Y77_002262"
     CDS             810..1484
                     /locus_tag="AC1Y77_002262"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIQ1575810.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1481..1822
                     /locus_tag="AC1Y77_002263"
     CDS             1481..1822
                     /locus_tag="AC1Y77_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575811.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1852..2211
                     /locus_tag="AC1Y77_002264"
     CDS             1852..2211
                     /locus_tag="AC1Y77_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HIQ1575812.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2471..2593
                     /locus_tag="AC1Y77_002265"
     CDS             2471..2593
                     /locus_tag="AC1Y77_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575813.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            2735..4816
                     /locus_tag="AC1Y77_002266"
     CDS             2735..4816
                     /locus_tag="AC1Y77_002266"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIQ1575814.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5127..6593
                     /locus_tag="AC1Y77_002267"
     CDS             5127..6593
                     /locus_tag="AC1Y77_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIQ1575815.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2188 a         1075 c         1410 g         1937 t
ORIGIN      
        1 ggaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
       61 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
      121 tattctaaaa aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa
      181 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
      241 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt
      301 gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta
      361 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
      421 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
      481 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
      541 ctgatggcgt catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac
      601 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
      661 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat
      721 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
      781 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
      841 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
      901 ttttgattgc cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc
      961 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     1021 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     1081 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     1141 aattactaaa tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg
     1201 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     1261 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     1321 atgctttagg gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     1381 cgattggcgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     1441 tttttagtgg agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc
     1501 agtaaaagaa gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     1561 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     1621 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     1681 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     1741 agaaatccaa aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa
     1801 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt
     1861 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     1921 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     1981 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg
     2041 tcattgattg ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat
     2101 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     2161 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata
     2221 ataaaggtta gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc
     2281 gttctcctgt ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg
     2341 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     2401 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     2461 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     2521 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     2581 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     2641 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     2701 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     2761 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     2821 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     2881 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     2941 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     3001 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     3061 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     3121 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     3181 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     3241 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     3301 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     3361 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     3421 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     3481 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     3541 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     3601 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     3661 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     3721 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     3781 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     3841 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
     3901 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
     3961 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
     4021 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
     4081 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
     4141 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
     4201 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
     4261 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
     4321 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
     4381 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
     4441 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
     4501 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
     4561 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
     4621 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
     4681 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
     4741 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
     4801 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
     4861 ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat gtttttaatt
     4921 ttgcagaaaa ttcaaaatag cttgaaattc aataaggtaa atgaaataaa agaaaataac
     4981 ttattttttt gatggaaaaa atatgaaaaa acgatgtgta ttaaataaaa gaatacacac
     5041 ataaaataac actgtcttgt aagtttgtta aaaattgaat atgattacat ctggataaca
     5101 aatttttatc ataaaggaga gaacgaatga acaacaaaca attacgctgg tttactgttg
     5161 cgttaattgc atttaacatg gtttggggca tggggaatgt agttaataac tatgctcagc
     5221 aaggagtatc agttgtcacc tcttggctat tgattttggc catttatttt attccatacg
     5281 cattgatcgt tggacaatta gggtcagcct tcaaggatag taaaggaggc gtcagttcat
     5341 gggtcgaaaa tacgacatct aataaacgac tggcatacta tgccgcttgg acttactggg
     5401 tcgtccatat tccttatttg gcacaaaagc ctcaagcaat cctgattgct ggcggttggg
     5461 cagtccaagg aaatggaaat atcgtaaata cgatgtcagt acaaatggta gcagttattt
     5521 cacttattat atttcttgcc tttttatatt tatcaacaaa aggactttct actttgaaag
     5581 tgattggcgg actggccgga acagcgatgt tcgtcatgtc gatcttattt attcttttag
     5641 ctgtaacggc accgttcatc aatccaacga tggaatttgc cacaccacat atgaatcaag
     5701 tgaaaacata tattccaaca tttgatttca gttatttcac aaccgtttcg atgctagtat
     5761 ttgcagttgg aggagcggaa aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa
     5821 aggaatttcc taaagggatg atcttcttag cagcaatggt agggatttgt gctgtattag
     5881 gctcagtcgc aatgggtatg ctgtttgcta gcgataatat tcctaaagac ttgatggcaa
     5941 atggggcata cagtgctttt caaattttag gtaattacta tggtatcggt aactcattga
     6001 tgatcattta cgcattgaca aacatgattg gacaaatctc cgcattagcc ttttcaattg
     6061 atgcaccact aaaaatcttg ttagcagatg cggatccaga atttgtacca aactggctac
     6121 gtaaacgtaa tcgtaaaggt acattggtga atggttatct tctgacaggt atactagtag
     6181 gaatcattat tttgttgccg atctttggta tcaaagatat gaatgaatta gtaaaatggt
     6241 tgacgaactt gaactcagtt gtcatgccaa tgcgttacct atgggtgttc ttggcatata
     6301 tgatgttgaa taaagcttat aagaaattca attctgaata taaatttgtt aaaaatccaa
     6361 aaatcggttt tatctttggt gcttggtgct ttgccttcac tgcgtttgca tgtatcctag
     6421 gaatggtgcc aaaagtagat tatactgctg atcctaaagc atggtggttc caattattca
     6481 cgaatatcat cacaccaatt gtcttgatct tattaggtat gatcttgcca gcaattgctc
     6541 gtcgagacaa aaacaaagaa atcactgaac cagaaacagt ggttcagcct taaaatagaa
     6601 atacttgtta
//
LOCUS       DBNIBO010000133         6563 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.138, whole genome shotgun sequence.
ACCESSION   DBNIBO010000133 DBNIBO010000000
VERSION     DBNIBO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.138"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(254..541)
                     /locus_tag="AC1Y77_002268"
     CDS             complement(254..541)
                     /locus_tag="AC1Y77_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1575816.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(531..860)
                     /locus_tag="AC1Y77_002269"
     CDS             complement(531..860)
                     /locus_tag="AC1Y77_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1575817.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            1165..1494
                     /locus_tag="AC1Y77_002270"
     CDS             1165..1494
                     /locus_tag="AC1Y77_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1575818.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(1781..2266)
                     /gene="vanZ-A"
                     /locus_tag="AC1Y77_002271"
     CDS             complement(1781..2266)
                     /gene="vanZ-A"
                     /locus_tag="AC1Y77_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HIQ1575819.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2419..3330)
                     /gene="vanY"
                     /locus_tag="AC1Y77_002272"
     CDS             complement(2419..3330)
                     /gene="vanY"
                     /locus_tag="AC1Y77_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HIQ1575820.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(3758..4366)
                     /locus_tag="AC1Y77_002273"
     CDS             complement(3758..4366)
                     /locus_tag="AC1Y77_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HIQ1575821.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4372..5403)
                     /gene="vanA"
                     /locus_tag="AC1Y77_002274"
     CDS             complement(4372..5403)
                     /gene="vanA"
                     /locus_tag="AC1Y77_002274"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HIQ1575822.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(5396..6364)
                     /locus_tag="AC1Y77_002275"
     CDS             complement(5396..6364)
                     /locus_tag="AC1Y77_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HIQ1575823.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         1955 a         1360 c         1085 g         2163 t
ORIGIN      
        1 ttgatgatct gtctaactta atttgacaat taagaattac taaaaaacaa tataaaataa
       61 cgcattttaa gccctctttg tgcataagga tgaagctttt aatttgaaag aggcttaaaa
      121 tgcgttttaa ttttttctat ttttcataaa tatataggtt agtggtttaa aattataggg
      181 tattacaact ctcagctgtt aaaaattatc ttttaaagtg tcgtctatcc tctcaagttg
      241 aaacattggg ttcttaccta atctttttta attgttcata aaaattttca tgcgttccac
      301 atagtaagtt aggtatcact gcattatctt taatttcata tgcaacgcga tatgtcgtac
      361 ctgcatactt gaatccaata gcccaaattc ctgataaatc acccgttttt tgttctcccg
      421 aatgaggacg aactgctatc tcatcataaa ttatcgttaa aaattgttct tttaatgttt
      481 tctcttttaa tttttttaga tactttatta cgtgtttaga aggtagaatc tcataagcca
      541 atttctgctg ccaactcctc ttttgtcatc ggtttagaat ttgcaacagt atcttcagca
      601 atagatttaa atgcagaagt taacgcattt tttctatttc taaattcttt taagatctct
      661 tgtttgctat aaccttctga aagaatatca gccaaaatat cttcactaaa atcaaaaaat
      721 tcttttttag gctctacaaa ctcataaagg ataccattct ctacaagctt agcttctact
      781 tcaacaccat tgggcacatc gaattcttta gggaccgtta acataataga atttccttgt
      841 tttcttgttt ttgtaatcat attatttcct ccattatttt acatttcgta attacttagt
      901 aattacattg tacgctattt ttagtagaaa tacaaaataa taaaactcct ttagaacaat
      961 tttcaaagga gttttatttg attaggaaat tatgtagaat aataatatgt ttttaacttg
     1021 gaatcaaaaa cgcacttgca agtaagttta cttcaataca tgaaattgct tataatagtg
     1081 tattggaatt aaggtagatt gacaaaagag tgatcaaata ggtatacttc attcaaataa
     1141 tcgtttgaat gtgaggtgag atgaatgaaa aaagtttgcc aagtgactgt cgtacataaa
     1201 gaaaaagtaa atggaataaa aaataaattg gatcaaaaag atttttcaaa cttacttgtg
     1261 ttagggaaat gtttcagcga tttttctaga attaaaattt tctatgcttt agaaacctat
     1321 aaggaaatgt gcgtctgtga tttagcagaa atacttactg ctagtgtcgc tacaacgtca
     1381 caccatttac gttttttaaa aaaacatgga atggcaaaat cacgtcaaga tggaaaagta
     1441 gtttattact ctttagcgaa tgaagacatc ctttccgcta ttcgtgtttt ttaaagttat
     1501 cagaaaatct atctatgaaa tcttagtttg ggggtaccgt caggaaaatg cggatttaca
     1561 acgttaagga aatttattaa agagaccaat tcctcagcac taaaattgat aattgggctt
     1621 tctttgttac tatagtgtta ggtaagcttt ttactttatc accggttttc ccctcacttc
     1681 acacctacgg gcgagtttcc ccacatcccg ttttccatct aatccaattc attcaatatc
     1741 tttatgtgta tgaaagatca gattgcttga tttagacatc ttacacgtaa tttattctta
     1801 aatgggtacg gtaaacgagc aataatacga gcaaaagtat acctacaaaa ataataactc
     1861 tgtctaattt tttttgattc atatgcttat tgcttaaacc atataatttc agtccaagaa
     1921 agcctccaac agtatttgta attacatctg ttatgtctgt cgctccaata gcgaagataa
     1981 attgaattat ttcaaaagta agacttaaaa ccagtacaaa agcaaactta ggtaaaaatc
     2041 cgatttcttt aaaattgaca ttcaaaagca agccaaatgg aataaagatt ataacattat
     2101 ctatcatctc tctgaaattc ccagtagcag taaatggagt caagttaaga cttctttgat
     2161 gataattaaa tactgataaa atattgtatt gtaatttgaa taacactaac cagattagtg
     2221 tcactaaata taaagctagc aatcctctag ataatatttt tcccaatata atttcctcta
     2281 attcttttgt ttaagttgta tcattagtat agcaatgtcc tagttaacgt gggttttaaa
     2341 ttaaaaagct aacgttaccc caaaagtgct agtacaataa tgctgttcat taatttcgtt
     2401 ggtttcattc cgccatcctt acctccttga attagtatgt gttgatccgg gaaacactgt
     2461 cacaattaca ccgtctatat tgtttcctga tatctcataa cgaagattag tcggcacatg
     2521 aatggtggta tttttagtaa caggataata aaagatctca tatttttccc catttacact
     2581 aacagaaatg gttttttctt cttttaggta atccatatat tcctcgagaa cgaaattctt
     2641 ttctttcata atcgcactat gtggtaaacc aacatagcga atatgccata gttcatattg
     2701 aattcctgtt aactctgttt tgtcctctgg ataacgtaaa atgaacccgt atttccaagc
     2761 attttcttct atccactttc cttcaggggc tcgttccatt ttcgtcaagc ttgatcctac
     2821 atctagtgat aaacctgaat tatgctcact ataacctgct ggtaaggcat actcagcccc
     2881 catttcttgg taaagcacac tttgctcatc aaagtctcga tagccactat taataataaa
     2941 atgactaacg ccacccttta cagcatcatt gaccatctct gaaaattttt gtgctatttc
     3001 ttttgacata taaatattac tatcaagcaa cccgtatcca tttattaatt cgtcatgttt
     3061 agataaattc acgatatctg acttcacact ttcttggcga acaggatatt tactattgat
     3121 taatagcaga tttccttgat aaacctgttc ttctgtaatt gttttctctt gggtattttc
     3181 agaagtccca ctattttcta aatgttcttt gggattttga tcataatttt gaaattcgac
     3241 tttttcctga gaaaacagtg cttcattaac gtagtcataa cctaagtata ttaagaataa
     3301 caataacaat aaaaaaaaca acttcttcat tttcagtctc ctccttaact atatagttgg
     3361 aagtatgaaa tatttctaga atcttcccct taaacggcac aatctcgttt gagattgtgc
     3421 cgttttgccc cgtaaattac agtcaattat ttcgccctac tggatttact attatcacca
     3481 atgtagatga attttcataa gtgataccca tgctgaattt aacgaggctg aactgcctgt
     3541 tgacaatggg cggtgtgaaa taccacccct ctatgcgatc agtcttatta tcaggctatc
     3601 ttgccgcagc gcacgcagat aatgaccgca atatcgctct gccttacatc ttcaatacta
     3661 tctactatat ataaattccc taagaaccag ttacatacgt cgggtttcct gccgaaagag
     3721 ttagcttata tagtttgtcc gtgcaacggt taaaagttta tttaacgggg aaatcaaaat
     3781 agctattggg gtatggttcg tctcttaata catagtgcca ccattcgagg ctatatgctt
     3841 caaacccact gttttccatg atggagcgca aacgtctgcg attttgcgct tcattgcatg
     3901 atattccatt tgccgcatga tgagagcgtt catccataaa atcaaatcgg ctccccattg
     3961 gtacaagctc acccgtgtct aatcgataaa gcgtaagatc aatggcactg ccgcggctat
     4021 ggcttgattt tgaagccacg tatccttttg aaatcatctc agttcggtca atattgggat
     4081 aataactttc ctttgtcagg ttattttccg gctgtgcagc ccattgcata aaacagttta
     4141 cagcacgatt aggacggtaa ccgtcccata gaagcaatcc gtacccttgg gtagcagcca
     4201 gttcttttgc cttcaaaagc gattcagcca actcgtatgt ccctacaatg cgatttactt
     4261 cataaccgtc aaccggtttt ccggtgaaat tatcccaagt ggcatattta gcgtcccaac
     4321 gaacaccgtg tactatttca tctaaaaaag taaatcctat ttccatgctt atcacccctt
     4381 taacgctaat acgatcaagc ggtcaatcag ttcgggaagt gcaatacctg cagcggccat
     4441 catacgggga taacgactgt atgacgtgaa accgggcaga gtattgactt cgttcagtac
     4501 aatgcggccg ttatcttgta aaaacatatc cacacgggct agacctctac agccgagcgc
     4561 tttatatatt ttttttgccg tttcctgtat ccgtcctcgc tcctctgctg aaaggtctgc
     4621 gggaacggtt ataactgcgt tttcagagcc tttttccggc tcgacttcct gatgaatacg
     4681 aaagattccg tactgcagcc tgatttggtc cacctcgcca acagctaacg cggcactgtt
     4741 tcccaatacc gcacaaccga cctcacagcc cgaaacagcc tgctcaatta agattttgct
     4801 gtcatattgt cttgccgatt caattgcgta gtccaattcg tccgcgctat tgactttttt
     4861 cacaccgaag gatgagcctg aacgcgccgg cttaacaaaa acaggatagg taaacgtagc
     4921 tgccaccggc ctatcatctt tattaataac ccaaaaggcg ggagtagcta tcccagcatt
     4981 tttcgcaacg atgtatgtca acgatttgtc catacaaatt gctgagcttt gaatatcgca
     5041 gcctacaaaa gggataccgg acaattcaaa cagaccttgt atggatccat cttcacctga
     5101 cttgccatgc aaagctgaaa atgctacatc aacatggttg atttcatatt catggttctt
     5161 tttaacaagt aatccgtgca tttttttatc cggcgagagt acagctgaat agcaattgtc
     5221 gttttcccat tccgcgcaag gtttttcgca cattttccat acaccagatt tcgtaattcc
     5281 aatgtataac ggctcgtatt tttctttatt aatgttagcg gctatctcta ttgcagattt
     5341 taccgatacg tcatgctcct ctgagcaacc cccaaacagt attgcaactt ttattctatt
     5401 catgctcctg tctcctttca aaatccaaac agtttttaat ggttttttca acggtatcac
     5461 gcaacgcttg ctcggtataa taggccgtat gcggtgtgat tatcacgtta ggcattcttt
     5521 gaagtttaag taaaaattga ttatcaattg gtttttgggt gcaatcagag tagaaaaact
     5581 cttcctctcc ttccaataca tccaatgcgg caccgcccag tttcccgttt tctaatgctt
     5641 taaccaactc ataggtatct acaagtggac cgcgcccagt attgataaga aatgctcctt
     5701 gcttcattct ctgtatttgt tcgtggctga taatatagtg cgtatccgta ttgagcggca
     5761 catgaagcgt aacgatatcg ctattttgca gcaactcatc aaacggtaca tagtttacct
     5821 ctatacttcg gctgcgacta taagccaaca ctttacatcc aaatcctcgc agccgctcaa
     5881 taaccgcttt gcctatctgg cccgttccca ccacaccaac tgtcatgtcg ctgagtacct
     5941 tgccacggtc gctgtccaac ctgaaatcat gtttttccac agagcgcaca atcgatttta
     6001 cgttgcgtac tgccataaga attagcatca tagtataatc ggcaacgcta tccggcgagt
     6061 acgccacatt gtcgacagtg atgcccattc tcttagcagc agttgtatct atatgattgc
     6121 agccgatgct tcgggtagaa atatatttca caccggctct cttcagcgca agaagaatag
     6181 aggcggaaat ctctgattta tgtcccacac tgatacattg attgaaaggc gcggatttgg
     6241 cgttggattc cgacacgttg gcgttaatta tcgttgccat aacgccaaag cgaggcgaaa
     6301 gagcatggaa tgcatctgcc tcatcctgct cacatccata aacagtaatg ccgatgttat
     6361 tcataattaa gaccaaccct ttctgtgaaa ggcaccaaat aaaacgggga tgccaatggt
     6421 aatcttgtca aatctttctc tgaagaacga aaacggctcg ttcttacggt attaagtcta
     6481 ccgtgtaaga acgagccgtt tcttaatatt aaattgttaa tttcctaaga aaaagtaaag
     6541 ataaccttga gattttccta aaa
//
LOCUS       DBNIBO010000134         6317 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.139, whole genome shotgun sequence.
ACCESSION   DBNIBO010000134 DBNIBO010000000
VERSION     DBNIBO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.139"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            221..655
                     /locus_tag="AC1Y77_002276"
     CDS             221..655
                     /locus_tag="AC1Y77_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIQ1575824.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="AC1Y77_002277"
     CDS             678..998
                     /locus_tag="AC1Y77_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575825.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="AC1Y77_002278"
     CDS             1139..1330
                     /locus_tag="AC1Y77_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575826.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="AC1Y77_002279"
     CDS             complement(1401..1658)
                     /locus_tag="AC1Y77_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HIQ1575827.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="AC1Y77_002280"
     CDS             1816..2079
                     /locus_tag="AC1Y77_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575828.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="AC1Y77_002281"
     CDS             2103..2291
                     /locus_tag="AC1Y77_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575829.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="AC1Y77_002282"
     CDS             complement(2374..2559)
                     /locus_tag="AC1Y77_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575830.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="AC1Y77_002283"
     CDS             2774..3145
                     /locus_tag="AC1Y77_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HIQ1575831.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="AC1Y77_002284"
     tRNA            complement(3295..3367)
                     /locus_tag="AC1Y77_002284"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="AC1Y77_002285"
     CDS             complement(3413..3841)
                     /locus_tag="AC1Y77_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575832.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="AC1Y77_002286"
     CDS             complement(3901..4659)
                     /locus_tag="AC1Y77_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIQ1575833.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4954..5151
                     /locus_tag="AC1Y77_002287"
     CDS             4954..5151
                     /locus_tag="AC1Y77_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575834.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5257..5739
                     /locus_tag="AC1Y77_002288"
     CDS             5257..5739
                     /locus_tag="AC1Y77_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575835.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDETEIDIMIDPE
                     "
     gene            complement(5797..>6317)
                     /locus_tag="AC1Y77_002289"
     CDS             complement(5797..>6317)
                     /locus_tag="AC1Y77_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIQ1575836.1"
                     /translation="ELTTLIKEAIKESPTAFNAQSTRAVILFGDAHEKLWEITEEALR
                     PLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTDVVKGLQEQFELYADNFPDWSEQSNGI
                     ATANTWVALVDKGLGANLQHYNPVIDEAVAKEWNIPSNWKLRSQLVFGSPETPAGEKE
                     YMNDADRFRVFG"
BASE COUNT         2135 a          979 c         1248 g         1955 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     4741 gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa
     4801 tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga
     4861 tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt
     4921 ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga
     4981 gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca
     5041 aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta
     5101 ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa
     5161 ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc
     5221 ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta
     5281 ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt
     5341 tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc
     5401 atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca
     5461 gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt
     5521 ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat
     5581 cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg
     5641 atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac
     5701 gatgagacag agattgatat tatgattgat ccagaataaa aacagaggtt gcgacaagat
     5761 gtttgtcaca acctctgttt tttatttcaa ataaaattat ccaaatacgc ggaagcgatc
     5821 ggcatcgttc atgtattctt tttccccagc cggtgtttct ggtgaaccga atactaattg
     5881 cgaacgtagt ttccagtttg aagggatatt ccattctttt gcaactgctt cgtcaataac
     5941 tgggttgtaa tgttgcaagt ttgcgcccaa tcctttatca actaatgcta cccaagtatt
     6001 agctgttgcg attccgtttg attgttctga ccaatctggg aagttgtctg catataattc
     6061 aaattgttct tgtagacctt ttaccacgtc agtatctttg aagaataata ctgtaccata
     6121 accatttttg aaaccagcta atttgttttg tgtatttggg aaagcttctg ctggagtcaa
     6181 tgggcgaagt gcttcttcag tgatttccca aagtttttca tgtgcatctc cgaaaaggat
     6241 aactgcgcgt gttgattgag cattgaaagc agttgggctt tctttaattg cttctttgat
     6301 caaagttgtt aattctt
//
LOCUS       DBNIBO010000135         6264 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.140, whole genome shotgun sequence.
ACCESSION   DBNIBO010000135 DBNIBO010000000
VERSION     DBNIBO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.140"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(79..864)
                     /gene="phnX"
                     /locus_tag="AC1Y77_002290"
     CDS             complement(79..864)
                     /gene="phnX"
                     /locus_tag="AC1Y77_002290"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HIQ1575837.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(857..1948)
                     /gene="phnW"
                     /locus_tag="AC1Y77_002291"
     CDS             complement(857..1948)
                     /gene="phnW"
                     /locus_tag="AC1Y77_002291"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HIQ1575838.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2165..2644)
                     /locus_tag="AC1Y77_002292"
     CDS             complement(2165..2644)
                     /locus_tag="AC1Y77_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIQ1575839.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            3003..3785
                     /gene="rpsB"
                     /locus_tag="AC1Y77_002293"
     CDS             3003..3785
                     /gene="rpsB"
                     /locus_tag="AC1Y77_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIQ1575840.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3884..4765
                     /gene="tsf"
                     /locus_tag="AC1Y77_002294"
     CDS             3884..4765
                     /gene="tsf"
                     /locus_tag="AC1Y77_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIQ1575841.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4901..5623
                     /gene="pyrH"
                     /locus_tag="AC1Y77_002295"
     CDS             4901..5623
                     /gene="pyrH"
                     /locus_tag="AC1Y77_002295"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIQ1575842.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            5626..6183
                     /gene="frr"
                     /locus_tag="AC1Y77_002296"
     CDS             5626..6183
                     /gene="frr"
                     /locus_tag="AC1Y77_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIQ1575843.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         2080 a         1162 c         1270 g         1752 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agtacagctt tttcacgacc
       61 tctgattttg tataaaagtc attctggatt cttgtcagcg tacaaaatgt attctgttaa
      121 ctcttttaaa ttgtttacac aaatatcagc gccagcttgt tggaatctaa gagcaacaga
      181 ctgttttaaa cgatcttttt tccccatctc taatttttgg tactcatcat aagaaatccc
      241 cattagcgaa ctaccttcaa ttacacctac tgtcaacact ccggcatttt tcccttcctg
      301 gatatctgag accgtatctc cgactttgat tacttcatca accgacttgt ttttgaagta
      361 ctgcatgttt ttatggatca tataaggaaa cggtcgacca tacccttcga cgtcttcaga
      421 agttgccatg aagtctggtg aatacccttc tttttttgcg gtttctgcta cgattgtcat
      481 cataggagca gtaaatccag tcgtggaacc aatattgatc ccagcgtttc tcaaccgttc
      541 gatcgtttcc aatgtgtcgg gtttcaaggt agaatgcttg accaaatctt taaacagata
      601 atgttcaaat tgttgataca attcctccac ctctttttcc tgaggtagtt gtccataaat
      661 agctatccat tgttttttga cagaagggag ggaaagaagc tgttggatat gatcccattt
      721 taattttccc atcggttcgc ggatttcttt gtcagtcagt tggattcctt tttctagaaa
      781 agcatttcta aaagcgtgga caggagccat acagccaaag tccacagttg ttcctgccca
      841 atcaaaaatt acagttttaa tcattaacgt tctccttata gtctttgata attgctgtca
      901 gttccaaaat atcttccgga tagatctcgc caatatttcc gatacgaaaa cattcatgat
      961 cgctgatttt cccgggatag atcacaaatc cgttttgctt taaatactga taaaaatgat
     1021 taaaatcaaa tcgccagtcc ttagggtaaa gaaaagaggt gatgaatggc ccctgatatt
     1081 cttgaatgaa aggctggaac cccatctctg tcattttttg tcgtagtaca tgattattgg
     1141 cgagataacg tgaatatcga gtttcgattc ctccttctgc ctctagttcc ttcagcgctt
     1201 gttgaaaagc caaaaccgta tgggtagggg aagtgaaacg ccatttgcca ttctcgtttt
     1261 ccatcacgcg gtactggtca tatagatcaa gtgacaaact tcgactcaca ttttttcgct
     1321 tttctagttc cgctttatta gcaagtacaa aagaaaagcc tggaacacct tgaatacatt
     1381 tgtttgagct ggaaatcaaa aaatcaatcc ctagttcggc tacacgaatc ggtatcccac
     1441 caaagcttga catcgcgtcc actataaaca aaagatcttt ttcttgtgtg acagcagcaa
     1501 tctcttccaa attattcaaa ataccggaag tagtctcgct atggaccatt gctagtgcgg
     1561 agatagaggg attctcttca atcagctgtg taatcacatg agcttggagg atttgatgtt
     1621 ccgatacctc ataaacggta tagttcagtt tcagacattc gcacatgttg atcattcgtt
     1681 gtccatatgc gccgtttgaa caaatcagta cagtatcttc tggcctgatc acagaactta
     1741 ataccgattc taccccaaag gagccgctac cttgcattaa gaccgttgtg tatattcctt
     1801 ccgatacttg agcaagatcc aataattttt ttcttatcca ttgtgtaatt tctttgtatt
     1861 cttgatccca agtacaatga tcgatcagca tagcattttt cactgtttca gaagtcgtca
     1921 atggtccagg agttaataat ttatacatat gatttccttt catttctttt ttgaaataca
     1981 caaagagcaa gaagctgctc ttgttaacat ccaatataaa tgatgattat tgtgattcaa
     2041 tcaaggtgtt gaaaaaattg aataaaaaat gatgtatttt tgtaaagagt gtaaaggttt
     2101 atttaataag gaaaatattg aaaataaaaa aacacgcatt ccatattagg aatacgtgct
     2161 ctgattaaaa aagtgatact tcagttggat ggtgaagaaa ttcttctact cgttttgcgt
     2221 cctcctcatt ttccgagatg accatgatcg tatcgaatcc agcaattgta ctgacaatag
     2281 agggaggttt gatctcatct aaaacagatg caaataaatg agcattgtct ggcaaggtat
     2341 tgacgatcgt caaaaagtgg actcgttcga ctttcaatac aatgtcttca atcatttgaa
     2401 ccagccgatc ctcttcctca gtcctagtct caggagttcc tttttcgcga aacaatacga
     2461 agcgggaaac gccattttca tcaggtgcct tcacgatttt tagatcacgg atatctcgag
     2521 aaatcgttgc ctgcgtggcg gtaacgccat aatgcccaag tttgtttaat agttcttctt
     2581 gcgtacggat cgggaattct tcaatgactt gtttaatcag caaatgtctt tgagctttgc
     2641 gcattttctt tctcctttac gaaagatttc acatgtatca taacaaaaaa aagaaggatt
     2701 gcccaataat attcgtcttt tatacctaaa attattttat ggaaaaagga taataaagaa
     2761 acaaagagaa atctactgtt tttcttgaat tctttttatc tttcaggtaa actattgaat
     2821 gttgagcaga aaagtatatt tgctcttgcg atacggatct tttgtctgta taatagctga
     2881 gggtgcttta tgcatcagca aatccacatt cttattggat tgtcggtagg tgcttgaaaa
     2941 agtttgccgg caaggtagaa gataagaaga ggaaaaataa aaccaaatcg gaggaaacaa
     3001 acatggcagt aatttctatg aaacaattac tagaagccgg cgtacacttt ggtcaccaaa
     3061 ctcgtcgctg gaatccaaaa atgaagaaat atatcttcac agaaagaaac ggaatctaca
     3121 tcattgactt gcaaaaaaca gttaaattag tagatgcagc ttatgattac atgaagagcg
     3181 ttgctgaaga aggcggcgta gcattattcg taggtacaaa aaaacaagca caagaagcaa
     3241 tcaaagaaga agcaactcgt gcaggacaat acttcgttaa ccatcgttgg ttaggcggaa
     3301 cattgacaaa ctgggatact atccaaaaac gtatcagccg tttgaaacaa atcaatgcaa
     3361 tggaagaaga tggaactttt gaagttctac ctaaaaaaga agttgctggt ttgaacaaag
     3421 aacgtgaacg tcttgaaaaa ttcttaggcg gtatcgctga tatgccaaga atcccagatg
     3481 taatgtacat cgttgaccca cgtaaagaac gtattgctgt tcaagaagca cacaaattaa
     3541 atatcccaat cgtagctatg gttgatacaa actgcgatcc agatgaaatc gacgtagtaa
     3601 tcccttcaaa cgacgatgca attcgtgccg ttaaattgat tacttcaaaa atggctgatg
     3661 ctttcatcga aggaaaccaa ggtgaagatc aagtagttga agaagatttc acagaagaaa
     3721 acacagcaac atcaatcgaa gaaatcgtag atgttgttga aggcgacaat tcttcagcag
     3781 aataattttg aatgatggag ctgtttcaaa ggctgggcga caagcctcac tctcctttga
     3841 agcagctttt tttaaaaaga aaaacacaca ggaggagtta aacatggcag acgttacagc
     3901 taaaatggta aaagaactac gcgaaatgac tggcgtagga atgatggatg cgaaaaaagc
     3961 attagttgaa gtagaaggcg acatggaaaa agcagttgat cttttacgtg aaaaaggaat
     4021 ggcaaaggct gcgaagaaaa atgaccgcat tgcagctgaa ggattggctt cagttgctgt
     4081 gaaaggtaac actgcagcaa tcgttgaagt aaactcagaa acagactttg tttctaaaaa
     4141 cgaaatgttc caagatttag taaaagaaat tgcagaactt gttgctgaaa acaaaccagc
     4201 agacatggaa gctgcaatga aaatcaaaac agataaaggt acaattgaat ctgacttgat
     4261 cgaagcaact caagttatcg gagaaaaaat cagcttccgt cgctttgaag ttgtagaaaa
     4321 agacgacaat gctgcatttg gtggatactt gcatatgggc ggacgtattg ccgtattgac
     4381 tgttctagat ggaacaactg acgaaacagt tgcaagagac gtagctatgc acgtagcagc
     4441 tatcaatcct cgttatgtaa acgaatcaca aattccagaa gctgaattag aacatgaaaa
     4501 aactgttctt acagaacaag cattgaacga aggcaaacca gcgaatatcg ttgaaaaaat
     4561 ggtagaagga cgcttgaaga aattcaaagc agaaatcgct ttggtggacc aaccattcgt
     4621 taaagatcct gatatgactg ttgaaaaata cgttgcatca aaaggcgcaa ctgttaaaac
     4681 attcgtacgt ttcgaagttg gcgaaggtat cgaaaaacgc gaagataact ttgtagaaga
     4741 agttatgagc caagtgaaaa aataattctg ttgaataaca gacgggaacg cacggtttgt
     4801 gcgctccctt tttttaagca aaacaagaaa aaccagtctt agagtgtcga aatcatctaa
     4861 gtatggtaaa atggcaatag aaaatattgg aggaatgaac atggtcacac cgaaatatca
     4921 gcgtgtagtc ttaaaattga gtggagaagc tttagctggg gatgcaggat ttggaatcaa
     4981 accaccagta atcaaagaaa tcatccaaga aatcaaagaa gttcacgagc tgggcgttga
     5041 aatggcaatc gttgtgggtg gcggaaatat ctggcgcgga caaatcggag cacaaatggg
     5101 catggaacgt gctcaagctg attacatggg tatgttagct acagttatga atgccttagc
     5161 tttacaggat acgttggaaa acgtgggtgt accgacacgc gtgcaaactt cgattgaaat
     5221 gcgtcaaatc gcagagccat atattcgccg taaagcagaa cgtcatttag aaaaaggacg
     5281 gatcgttatc tttgcaggcg gtaccggcaa cccgtacttc tcgacagaca caacagcagc
     5341 attacgagcg gcagaaatcg gcgcagatgt tatcctgatg gctaaaaaca atgtagacgg
     5401 tgtttattct gctgatccga aagtagatgc aaatgcagta aaatttgaag aactgactca
     5461 tctggaagtt attgccaaag gattacaagt catggattca actgcaagtt cactaagcat
     5521 ggataatgat attccactgc ttgtcttcaa tttaaatgaa cacggaaata ttcgccgtgc
     5581 aatcttaggc gaaaatatcg gaacaactgt aagggggaaa taaaaatggc agatgcaatt
     5641 atgacagaag caaaagaaaa aatgaaaaaa gcagcacaaa atcttcaacg tgaacttggg
     5701 caaatccgcg caggccgtgc gaacgctagc cttttggacc gtattactgt gaactactac
     5761 ggtgcgccaa caccattgaa ccaaatggca tcaatccaaa ttccagaagc acgtgtttta
     5821 atgatcacac cttttgacaa gagcattctt caagatgttg aaaaagctat tatggcaagt
     5881 gatatcggaa tcagtccaac caatgatgga aatgtcattc gcttagttat cccgcaactg
     5941 acagaagaac gcagaaaaga attagctaaa gatgtgaaaa aagaagcaga aaatgcaaaa
     6001 atcgcgattc gtaacatccg tcgtgatgcg atcgatgaat ataaaaaaca acaaaaaaat
     6061 ggtgatatta ccgaagatga tttgcgcgga ttagaaaaag acgtacaaac attgacagat
     6121 aacagcatta aagaagtaga taagatcgct gctgataaag aaaaagaatt attggaagtt
     6181 taataaaatc ataaaaacag aggggcggat cattccgctt cctctgtttt tatttatatt
     6241 tcttttattt aaaaattaaa aaaa
//
LOCUS       DBNIBO010000136         6213 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.141, whole genome shotgun sequence.
ACCESSION   DBNIBO010000136 DBNIBO010000000
VERSION     DBNIBO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.141"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            128..2917
                     /locus_tag="AC1Y77_002297"
     CDS             128..2917
                     /locus_tag="AC1Y77_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIQ1575844.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2964..3491
                     /locus_tag="AC1Y77_002298"
     CDS             2964..3491
                     /locus_tag="AC1Y77_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HIQ1575845.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3512..4702
                     /locus_tag="AC1Y77_002299"
     CDS             3512..4702
                     /locus_tag="AC1Y77_002299"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIQ1575846.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4742..6040
                     /gene="asnS"
                     /locus_tag="AC1Y77_002300"
     CDS             4742..6040
                     /gene="asnS"
                     /locus_tag="AC1Y77_002300"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIQ1575847.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2052 a         1151 c         1366 g         1644 t
ORIGIN      
        1 aaacgcccag acatttgcgt gtctgggcgt ttttgtctat tttctttaaa aagcgatgag
       61 agtatttttc tgcactttgt agtataattt caaaagaaca ttagaggcag gagaagagga
      121 agatattttg aaaccaagaa cttatgcagt cgttgatatc gaaacgactg ggacgaatcc
      181 aaaagaagat cggatcatcc aatttggctg cgtcatgatt gaatcaggtc ggatcacttc
      241 acgtttttcg atcgatattc atccagggcg gaagatttca aaacaaatcc agcatttaac
      301 agggatcacg aatcaacgcg tccaaaaagc gccttatttc gaagatgtcg cccaaacgat
      361 ttacaatctt ttggcagaca ccatatttgt cgcgcataat atttattttg attataattt
      421 tttgaaccat gaactcatgc gatgcggact tcctagtttg aagataccag gcatcgatac
      481 agtagaactt gcacaaattt tcttgccgac ggaaccaagc tttcgcttag ctgatttgtc
      541 agaaagcctt ggatttattc atgagaatcc tcatcaggca gatagtgatg ccgaagtgac
      601 gggtcaactt cttttgctga tcgaagaacg gatgagaaaa ttgccaatca tcacgttaga
      661 gcagattgcg cgattaagta gacacacagg tatggatacg agtcgtttca tttatcatgt
      721 gattgaagaa atgaaagaaa aaccagagcc acttgatccc tcattagaaa tcgtcgatgg
      781 gctagctttg cagaaaaaag aagtggaact attcacatcc gtccattatg gcgagcggac
      841 atatccaaga aaaaaacaag caaaagaaaa aatgtttggt aaaacactga tgtatcgaaa
      901 agaacagaac agactgatga atgcggtcta cgaccatttc acaaaagatg aatcaaaaga
      961 tttgatgatc gaagcagcta caggaatggg taaaacaatc ggttatctgc tgccattgag
     1021 ttatttggct acacctgaaa aacccgctgt catcagtacc gtttcgctag ttttgcagca
     1081 gcagatcata gaaaaagaca ttccattgct gaatcaattg ctagatcagc ccatacaacc
     1141 tgtaattatc aaaagctatc gccactatat tgatttacag cgttttaaag gcacacttgt
     1201 ggaaccgcca gaacaaaaac aatatgcttt gtatcaaatg gcggtcttag tttggctgac
     1261 gcaaacgaag accggtgatt tggatgaatt acatttaaca aatttgaacc attcattctt
     1321 tgcagatatc gcgcacagag gtattggatt tttagcaaga aatcagtcat tttacgaaca
     1381 agactttgtt cgtttcttgc aaaagaaaat caggcaaagt aattttttga ttgtcaatca
     1441 tgcgttttta atacaagaaa cacagcgaaa agaaccattg cttccagcta gtccattttt
     1501 gttgatcgat gaagtacatc atttgccgga aatcgctgaa aaagtaaatg acaaacgatt
     1561 aagtattaac tatttccata aacaaatcca gcaattagaa gaaccaaatc agctgtttga
     1621 ccgaatacaa agctatttac cgaaagaaca tgaagcctta cgctggttat cgatttatcg
     1681 agaagaactt tctgcgctat gcgaagcaca gatttgtatg gcagaaggat taatgagttt
     1741 agtgagcggc aagcaaccta acggacagta tccagaagaa gtcatgatga caaaagagat
     1801 gttggatcag ctgccactgg aatctgggac agccattgat cacctgcagc ttttttatca
     1861 ggagatccag cagctacagt cacaaatgcg tctgactttt gagattatcc aagaaagctg
     1921 gacaaaccag atgcggatcg atttagcaag tttatatagc ttatttgatc aattagacaa
     1981 gcagtacttg ctgatggatc aatggctgga agactggcga gatcatttga tccattggtt
     2041 cgttcctggg aacacagcgc agcagatgat ttttcatctc catgattttc aagcggctat
     2101 gttgccgtcg actgtttggt atcctcgata tgatcgtatt ctttatattg gaggaacgct
     2161 gaagatcggc cctaatcgac actatctctc taagcggctt ggcattgaag cagcacctct
     2221 taaagtgatt gcctctcctt atgattatga gaaacaggca aggatctatg tgccaaaaga
     2281 ggcacctgcc gtccatcaag tcagcaatca agcatatatc gattatttag agacagcact
     2341 tacaggtctg atcgaacaag aagatcggcc aatacttgtg ttattcactt ctcatgatgt
     2401 tttgcagaag gtctatcaga aaatgcatct gtctatgcta gaaaaaggac gagaaatact
     2461 agctcaaggg tttggcggaa gccgggagaa gcttttgaaa cgcttcattt tgtcagaaaa
     2521 aagcgtattg tttggcgctg acagcttttg ggaaggtgta gatttgcctg gggatgcttt
     2581 gcagatcgtt gtagtcacac gtttaccctt cgataatccg cttcgcccga tggtcaaagc
     2641 taggaacgct tatttagaat cagaggggat caatcctttt taccaggaat ccgtacctaa
     2701 agcagctttg aagttacgcc aagcattagg tcgcttgata aggacggaga cagatcgtgg
     2761 cgtattgctt attttggatc gaagactagt cactgcaaaa tatggtgcac ggatcatcca
     2821 tgcattaccg aaaaaagtac cgatcaaaga gctagctatg gaggaaatct atcaggacat
     2881 ccatgaattc ttaaagaaaa atgaagaagg aagttagagg agctgtcttt tttcataatt
     2941 aaaatctgct ataatgaaaa ggaatgaaga taaaggagcg acgacccttg aagaaaaaac
     3001 agacaaagga aaaaacgatt aatcgcgtgt tacttggctt gatcgtattt ttattagcgg
     3061 tcatcgtgat cagctcgatt ttttacttgc gttccagtcg tccggcggtg caggcgaaga
     3121 aagaagcagt agaaatcgca aagaaatatg caaatcttga ggaagccgat catttttatt
     3181 ggtttactcg aaagaagaca tacttttcac tgacaggtac aaatgataaa ggaaaagaaa
     3241 tcgccgttat cattcctaaa tctggtgaaa aagtaaaagt attggatcaa acaaagggac
     3301 tgactgaaaa cgaagcaaaa cagacaattg ctgcggccca tcctgaaatc caaatcgaaa
     3361 aagctgcttt agggatgtat gatgatcagc ctgtgtggga agtagtagga aaagaaacaa
     3421 atgggaaact gaattattac ctgattggct ttgaatctgg agatgaagtc aagacaatca
     3481 agggaattta aaaggtaaag gaatgaatgg gatggagtta tcaaaaagaa caaaacgatt
     3541 agaaccgtct gtcactttgg cagctgcggc aaaggccaag gcattaaaag caaaaggcag
     3601 agatgtgctg agtttgactg tgggagaacc ggattttgcg acaccagaaa acatccaaga
     3661 agcagcaatc gaagcaatcc gcaacgggaa agctagctat tatacaccaa cagcaggaat
     3721 cccagagctt cgacaggcaa tcgttgatta tctgaagaaa aactatggac ttgtctatga
     3781 accttcacaa acagtcgtaa cagacggagc gaaatttgct ttgtataccc ttttccaaac
     3841 gatccttgac ccgcaagatg aagtcatcat tcctgtgcct tactgggtca gttacggtga
     3901 gcaagtaaaa ctggcagaag gacttcctgt ctttgtgaaa ggggaagaat ccaatagctg
     3961 gaaagttact gttgaacagc tagaacaagc acgttcagaa aaaacaaaag ccgtaatcat
     4021 caattctcca tcgaatccga ctggaatgat ttatagcaag aatgaactgc aggcaatcgg
     4081 tgaatgggca gtaaaaaatg atattttgat agttgctgat gatatttatg gtcgtttagt
     4141 ttataatggc aatgagttta ctccaattgc aacgatttca gaagcaatca aaaatcagac
     4201 tatcatcatc aacggcgtgt ctaaaacata tgcgatgaca ggctggcgca ttggttatgc
     4261 ggtgggaaat cctgtgatca tcaatggaat gatcgctgtt gcttcccagt caaccagtaa
     4321 tccgacagca gtcagtcaat atgcagctgt tgaagcattg aaaggcgagc aagatacagt
     4381 ggaagaaatg cggaacgcct ttgaagaacg cctgaatacg ttatattcgc ttgtggcaga
     4441 actgccgggt gtttctcttg aaaagccaca aggagctttt tatctttttc ctaatgtaaa
     4501 agaaacgcta cgcatgtgta aatatgaaaa tgtcacacaa tgggtagaag atcttttaga
     4561 agaaacaggg gtagcgcttg tgactggcga aggctttggg gcaccagaaa atgtccgcat
     4621 gagttatgct actgatcgcg ctacgttaga agaagcggta cgcagaatca agcaatttat
     4681 tgagagtaaa agtcaaaatt aatcctaaag aaattcaagg aatataaaag gagagtcata
     4741 cgtggaacaa attcaaatca tcgattcgaa aaaccatgtg ggagaaacag taacaatcgg
     4801 cgcttgggtc gcaaataagc gatcaagcgg aaaaatcgca tttttacaat taagagacgg
     4861 aacagccttt ttccaaggtg tagttgtaaa aagcgaagtg tcggaagaag ttttccaaac
     4921 agctaaaaat ctacaccaag aaacatccgt gttagtaact ggagaaatcc gtgaagacag
     4981 tcgttcaaaa tttggttatg aaatcggaat tacaaatgtg gaagtggtaa gcgagagcca
     5041 cgattatccg atcacaccaa aagaacacgg aacagacttc ttgatggatc atcgtcattt
     5101 atggctgcgt tcttcaagac aacatgcgat tatgcagatc agaaatgaaa tcattcgtgc
     5161 aacgtatgaa ttctttaatc aacatcattt tgtcaaaatt gatccgccga tcttgactgg
     5221 ttctgcacca gaaggcacta cggatctatt cgaaacaaat tactttgatc aaaaagctta
     5281 tttatctcaa agtggccaat tatacatgga agctgctgct atggctttcg gaaaagtctt
     5341 ttcattcggt ccgactttcc gtgctgaaaa atcaaaaaca cgacgtcatt tgatcgaatt
     5401 ctggatgatc gaaccagaaa tggctttcac tcatcaagaa gaaagtttag aagtacagga
     5461 acaatatgta gcattcttag tacaaaatgt tttagatcat tgcgaatatg cattagatgt
     5521 actaggacga gacaaagaag tcttaaaaac ttatacaaaa ttgccattcc ctcgtattac
     5581 ttatgatgat gcgatcgaat tactgaagaa aaatggattt gatgacatcg aatggggcga
     5641 agactttggt tcacctcatg aaacgttcat tgcgaattca ttcgaacagc ctgtctttat
     5701 cctaaactat ccaaaagcca tcaagccatt ctacatgaaa ccacatccaa caagagaaga
     5761 cgtggtgatc tgtgcagata tgatcgcacc agaaggttac ggtgaaatta ttggcggaag
     5821 cgaacgggca acagactatg atttcttgct agaacaaatc cgtaatcacg gtttggatga
     5881 aaaagaatat tcatggtatc ttgatctacg taaatacggt tctgttccgc actctggttt
     5941 tggtctaggt cttgaacgaa cagtcacttg gattgctggt atcgaccatg tacgtgaagc
     6001 tattccattc ccacgtttgt tgaaccgaat ttatccataa gaggtgatga aaaagctgtt
     6061 ctgcatcaag taattagaac agccaagcaa aaacagctgt tcatgttttt gaattggcgg
     6121 gacttattac cgcagatgta agcttttgaa taccgtttag tggagaggtt gtgaaaaagc
     6181 tgtttagttt cgagtattaa gaaaaatttt gtg
//
LOCUS       DBNIBO010000137         6076 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.142, whole genome shotgun sequence.
ACCESSION   DBNIBO010000137 DBNIBO010000000
VERSION     DBNIBO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.142"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            481..567
                     /locus_tag="AC1Y77_002301"
     CDS             481..567
                     /locus_tag="AC1Y77_002301"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIQ1575848.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(688..1377)
                     /locus_tag="AC1Y77_002302"
     CDS             complement(688..1377)
                     /locus_tag="AC1Y77_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575849.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1384..1662)
                     /locus_tag="AC1Y77_002303"
     CDS             complement(1384..1662)
                     /locus_tag="AC1Y77_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575850.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1622..2536)
                     /locus_tag="AC1Y77_002304"
     CDS             complement(1622..2536)
                     /locus_tag="AC1Y77_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIQ1575851.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2518..2898)
                     /locus_tag="AC1Y77_002305"
     CDS             complement(2518..2898)
                     /locus_tag="AC1Y77_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HIQ1575852.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3348..3635)
                     /locus_tag="AC1Y77_002306"
     CDS             complement(3348..3635)
                     /locus_tag="AC1Y77_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HIQ1575853.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3655..3879)
                     /locus_tag="AC1Y77_002307"
     CDS             complement(3655..3879)
                     /locus_tag="AC1Y77_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HIQ1575854.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4329..4499)
                     /locus_tag="AC1Y77_002308"
     CDS             complement(4329..4499)
                     /locus_tag="AC1Y77_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575855.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(4514..5047)
                     /locus_tag="AC1Y77_002309"
     CDS             complement(4514..5047)
                     /locus_tag="AC1Y77_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIQ1575856.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5068..6012)
                     /locus_tag="AC1Y77_002310"
     CDS             complement(5068..6012)
                     /locus_tag="AC1Y77_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HIQ1575857.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1592 a         1136 c         1036 g         2312 t
ORIGIN      
        1 attctgtatc cattggtaga gtaaggacac agcttacggt aaagaggttt aaaaagaggt
       61 ttaaaaagag atataaaaag aggggggcta attttcccgc ctaaaaacca cttctttttt
      121 ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca aaaccttgct tcctacggtc
      181 gccaaaaagg tgtgatcgct acgctctcac attgtactgc gcagtcgcct aaaggctcct
      241 ttgctaaaaa catgttggta aagaggcctt tgagcggttc tgaatgctta tctaagccgt
      301 tttaagacag tcttggataa aaatccttat ctagctttaa agcgccttac aggcggtcag
      361 ggcaagccct aacaacccta aaataagcga tacttaaaaa caccacacaa gtttaatttt
      421 ttaagatata ataacttgtc gcctatccga cagtcttgcg actggaagga ggtgtatcta
      481 atgtttgaat tgttcatttg cccccttctt atagaagttt gtgtctcttt attcagttat
      541 tggttgaata accgaaacaa gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag
      601 ccccagagaa tccgacctct ctggggcttt cgtgtatcta atgtttgatc atttgcatgt
      661 ttagtttaac atgattgccg atcaaattca attttgctgt tgctgatcta accattcctt
      721 gacacttttt agataattta atgccgtttc ttttttcgtt ggattttgta tgttcgttgc
      781 ttgctgaagc agtgctttat aacgttcgta ggtaactgat tgttgccgtt cttttcgttc
      841 ctgttcacta gttgaaaaag ggattgtatc actgaataaa ttataacaag cttcttctgt
      901 ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc tcatagttgt cttgaacctc
      961 tttttgctgt ttttctggca agttttgata aagacgagtg agtttttcca tgtttttatc
     1021 cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt tcctcataac aagtggtaaa
     1081 ctcttccatt ttttgtgtta acacttgctc ctttttcttt ttatcacatg ctgcttcata
     1141 cttttgagtg atatccccta ataaaatact tcttgaaata cgtttatttc cctctaaaac
     1201 gccgaataaa ccacttaaaa cgattgataa tgtaaatata acccctggca ataaaaacct
     1261 cttaaatcga attttagagc cttttaatga atatcttgtt tgataagcct tatagctaag
     1321 ccaaataatt cctaaaatac tggcaaaaat gagtagatag ggtaaataga atgccatttc
     1381 tatttattct ccccatacca gccaaaaggc taacagtgtc atgattaagc aacataaggg
     1441 ggcaagaata tccattgatc gctgcataat tacttttttc tgttctttat ttcgctggtt
     1501 taatagcgca tggtggaatt ttttgacttg tgaatcaatt gtagtttgta ttctttcttt
     1561 ggtataaggt gcaagaaaag cacgttgttt ctttatttca ttttctatgt cttgtaacgt
     1621 tttagaacga taattcttgt tggtagtctg ctcctctttc aacgtgttgg tcatggtttc
     1681 ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt tcgttgttct cttcgtactt
     1741 cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt cgttgttgat
     1801 tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg taatcctttc
     1861 ctagtttgtt ggatcgtact tttttctttt tgtggatcaa ttcgtaagta aaatttttcc
     1921 cacgattcaa gcaatgaatg ccgaatgttt taagagaatc acaaaattgg ttatacgttc
     1981 gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct ttccaaacag
     2041 gttcattttt ttcttttaat ttgatttcag acatggttcg tttttcgggt ttcttccctc
     2101 gttcagtgat tgttaagtca tgttcaagac aaatctcgtc atttaggtca taacatttgt
     2161 ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt ttccagattg acggagttaa
     2221 tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt gtaaatcgcg atttggtggt
     2281 caggggctat ttttttcgca tactctgccc ctagttgatt ggctttttca gcggtaattt
     2341 ctcctggtcg aaaagactgg atcaataagt gtgcttgtac acgatcgtct tttccataca
     2401 gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat gtcacagttt atccctgttt
     2461 tgacggttgc tcgtggttcg gcgtaattga tcgctcggct acaagaagtg gttcggctta
     2521 ctttaactgt tgccatatct tttgtagctc cttttgcatg atttttaggt tttcggataa
     2581 gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg ttgcaccact tggcaatttg
     2641 attgatattc ccgccagctt tggctaaatc gcgcgcgatt gcctgagcaa tgtctggcgc
     2701 cactttcggt gtcaccagac gcgcgttttg tgccttggct ttcacgaaag ccggcacact
     2761 catttgcaaa gcttcagctg actgcttcag ctttgcaaat tcggattcgc tcactcgaaa
     2821 actgatttgt tttggctcgg atcgataggt atatttttta gaatgattgc tagccaaata
     2881 ttgattttgt tcgctcataa gtcccaccct ttcagtacgt cctacggacg tatcgttcgc
     2941 aataccattg tacctaaaaa ggtacaataa gtaaagctca ctaaagctca ctaaagggct
     3001 tggcaagctc tatatttgct agccaaatac ctacgtattt ggtgcaaata accgcttgtc
     3061 aaaggggcgc aagctccccc ttgaatcccc ctccccccca ctctccacaa tacaaaaaag
     3121 actagcaaat gctagccact ttttgtaaag tttcgcttgt ttcaccttcg gtgaaattgt
     3181 tgcaaatcaa taattaggta gagctacatt tttttcgttg ttcaaagatt cttatgccat
     3241 ttactattaa taaatctata aatatgataa tttttcggct ctataattta tgggactgat
     3301 gaatcagaat tgattcttca gtcccatttt cattttattc caaagaatta actccaaata
     3361 ccaatagaag cccatcctct atatacttga gattccttac taacaatttc ttgtaattct
     3421 attaaagatt taggtaatcc aaatttatgt tttaaaatat atcttccaag aaaactactt
     3481 aatttcatac taacttcacc aacacttttt ccctcactga tttctttaga ataaagaaag
     3541 agcttttctt ttaagtcagg atagtcatct atagttgctt cattgtatac tttactaatt
     3601 gcatttaaaa gtttttcttc tttagtaaaa tccattataa taacctccct accactacct
     3661 tctaggtgcc catggaccat gattaaccca accattaaca ataatttttc caatttctcc
     3721 tttagcttga ttccaatcta cccaacattt ttctttgtta caataaagac catttccata
     3781 gtaagtagct gcatcaactt ttattcctgt accgatgcca gctagacaag ttcctaatat
     3841 tcctagaata acacaatgtt ttaatacttt ctttttcatg attgtttttc cttttcgttt
     3901 ttttaaaatt gcttgttata ttaaataatc ttagtaattt tattatataa cattttatta
     3961 tataacaatg attaacgtca taaaaagtag ttttgaattc ataatcgttt aaataaaatt
     4021 ataagatcag ttatttaaac agctagtaga cataatgtac actatgccaa gtagcccctc
     4081 taccaatgga tagagacgct cactttgtga tttttaattg gtctaggtca aaagctcttt
     4141 ttaaggcttg attttctgca gttgcttctt tccgtttctt tgtgaatttt taccagtcat
     4201 tttttactgg ggtgttattt ttcataaaat atgataaatt tttggtagcg cttcataagc
     4261 gcgttgcttt ttcaagaata aacactcatt attttttaac tggctgctcg gaagagcagt
     4321 cttttttatt atttatccgt ttcttttaca aacaattctt cgttaggact gttgtagtaa
     4381 ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga taataacatt
     4441 gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt ttttgtcata
     4501 agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt gtttgttcgt
     4561 ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac tcttccgtat
     4621 aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc agggtgagtt
     4681 gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg agggcaagtt
     4741 gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt gcaaccacgt
     4801 ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc aacttatcta
     4861 gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc aatacaacct
     4921 tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata tgtttcgata
     4981 ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag gtttcttgtg
     5041 gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat cagataataa
     5101 actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag cttttttctc
     5161 tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct gtttgatttt
     5221 ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta aagccaatgc
     5281 tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt ttttgatctc
     5341 aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc cgtatttctt
     5401 tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt ttataactcg
     5461 tttatctata tcaggttgct tataactttt aggaatatct aataattcaa gaaagtcttc
     5521 tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa acatagtctt
     5581 tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc actcgtctaa
     5641 attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag gattttctac
     5701 gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa gcccagactc
     5761 acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta aatcttctac
     5821 aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt caaacgtaaa
     5881 acgaactgtc ttatcccctt tttcacgcat tctagacacg atggaaaaaa acaaattcat
     5941 ttcaaccggt gtaaattttc ttaaaggaat tgtatttaat tcatggtggt attttacaat
     6001 ctcattagcc atataatcac ctcaatccaa ttatgaatga cattttttaa atgtcaagac
     6061 attcatattt gacgtt
//
LOCUS       DBNIBO010000138         5975 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.143, whole genome shotgun sequence.
ACCESSION   DBNIBO010000138 DBNIBO010000000
VERSION     DBNIBO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.143"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            188..2281
                     /locus_tag="AC1Y77_002311"
     CDS             188..2281
                     /locus_tag="AC1Y77_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIQ1575858.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            2306..2608
                     /locus_tag="AC1Y77_002312"
     CDS             2306..2608
                     /locus_tag="AC1Y77_002312"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1575859.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            2635..3924
                     /locus_tag="AC1Y77_002313"
     CDS             2635..3924
                     /locus_tag="AC1Y77_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1575860.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            3943..4350
                     /locus_tag="AC1Y77_002314"
     CDS             3943..4350
                     /locus_tag="AC1Y77_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIQ1575861.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            4373..5242
                     /locus_tag="AC1Y77_002315"
     CDS             4373..5242
                     /locus_tag="AC1Y77_002315"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIQ1575862.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            5244..5864
                     /locus_tag="AC1Y77_002316"
     CDS             5244..5864
                     /locus_tag="AC1Y77_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIQ1575863.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
BASE COUNT         2016 a         1024 c         1268 g         1667 t
ORIGIN      
        1 tttttgtata gatgggcaaa atttttgtaa taataggcgt atgtcttttt cgttgaggag
       61 atgaaaaagc atatggctta ttttttgttt gctttgctta ctttctttcc acttcaagtg
      121 gcagaaatat ctacttttta ttatgtaagc ggaaccatat acttgaaatc agaaggagat
      181 catttctatg aaaacaagaa caattgaaat acttcacaga atcatgacag cccaatatcc
      241 agtaaaagca aaacatctag caagtgagtt caatgtcagt cagcggacga tccgtcagga
      301 agttcttgaa gcgaatatct ggttgagcag taaaaagctt ccggagatcc gtacaattcg
      361 caacaaaggt tttttgttga agctggaaaa ggaaaagcaa gctcgattag aaactgaaat
      421 aaaagatgtg aaagacggct ttttgaatcg gcgcgaacgt atttttgatc tagttttagc
      481 cgttgcttac agccgaatcc cgatccatct aaacaaaaaa gaagaagaat ttcaaatctc
      541 taaaagtaca ttagatgaag atatgagacg gttacgagca acactggtca gttatgggat
      601 agagatcgtc agttatggaa agcaaggact ggtttataaa ggttcagaac gatctatccg
      661 tacgatgatc tatgatttta tcaatcagaa tctgggaatg accgacttct ttatgaatca
      721 agatgcacaa ttaacaccgg ctcaaaaaat ctttctccat tattttcctt tggtggaaat
      781 caaaaaaata caggagatct attccaataa gttgttgaaa caagaagatg atatttataa
      841 aaaacaaatc ctgcttttta cattgatctg gatccagcga atcaggaaac atgaatttat
      901 ctctgccatt aattggaaaa atacagaaat aaaagacagt agcttttctg catttattga
      961 ccacatgatc gaggaatttc aattggacaa tgtcccgcaa gtagaaagaa attatcttcg
     1021 tttcactatt gagacgttca atgctagaga tatctccaat tcaatcgagt gggtacaagc
     1081 tcaattacta acgatccaat tgatccaatt tgtagagaac gagacacacg tacctttcca
     1141 tctaaaggaa gaagctttat tcgaaaatct ctataagcac atggctgcac tgattgtaag
     1201 aatcaagaat catatccaag tcatgaatcc attgaaagaa aacatccgaa caaattattt
     1261 cccaatctac cgcgcagtca ctcattttgt tcctacgatc gaagaagtaa ttggtggcaa
     1321 gatactggag gacgagatag cttttcttgt catccacttt tcgacgatcg ccagtgtaat
     1381 caagcgagac ttgaattatg tttataaatc agtcgttgtc tgtaaccatg ggatggctac
     1441 tggaaatctg ttagcagaga atctgaaaga aaagtttcct cagatcgaga ttactgcagt
     1501 tttaagttca aaagaaatcg aattagtaga taaattggat gtcaatctgg ttttcagcac
     1561 ctttcaattg aattatcgaa ataagccgct tttagttgtc gatccgattt taactgagac
     1621 gaatatcttg gttatagaag actttttgaa aagaaatgaa gaactacagc ggttagagcc
     1681 taacagtcat gaatcgacgg agttattcac tcaaataata gatgtcatga aagaaagtgg
     1741 tggccgagta gatcgaatca tctatggcaa gttagaaaaa ttattcgaaa caaacaattt
     1801 agaaatcaac aaaagggaga tccagcctat gttaaaagat attttgacgg acagcaatat
     1861 catgatcaaa gaaaaagcca ctacttggga ggaatcgatt gaactagctg ctagaccatt
     1921 ggttaaagaa aacattgtcg aagagcgcta tgtcgaagcg atgattcgtg cagtgaatga
     1981 ttacggaccg tatatcgtga ttggcaaaca catggcacta gcccatgccc gaccagaaga
     2041 tggggtcaat aaattaggag tcagcgtagc tacgattgag caaccaatcg atttcggtaa
     2101 tcctgaaatg gatccagtaa agattatttt ttgtttggca gcagtagact cctactcgca
     2161 tctgaacatc atgaaagaac taattgaatt gatcaacaac gaagagaagt tggatcgatt
     2221 gattgaatgc acaaatatcg aagatttcaa acaactgctt ttttcagtag ttgagaaata
     2281 ataaatgata cgggggagaa caaagatgaa caaattgaac attttattcg tatgcggtgc
     2341 aggtttagga agtagttttg cagcacagat gtctgctgag gatgttttga acaaattagg
     2401 agtagaagcc aaactggacc attgtgatat ttcttccgca gtttccatga aaccagatgt
     2461 gatcatcaca gcagaaaatt ttcgttcgca gttcgaaaag ttcgcagttg atcctaaaac
     2521 ggcgatgatc tatttgaaaa atattgtttc aaaaaatgaa attgaagaaa aaatcacccc
     2581 agttctgcaa gataagggag ttatctaaat acaaaaagaa aagagggtaa aaacatgggc
     2641 gttatcaatt ttatcatcga aaatattttg actcaggcat cgatcaccat tgcattgatt
     2701 gctatgttgg gtctactact gcagaagaaa tcagcaggac aagtcatctc tggcacgtta
     2761 aagacactcc taggatttca agtgttgagt gctggttcga gcatcatcgt tggaagttta
     2821 acttatttcg ggaaaatctt tactgaagga tttcatatgc aagggatcat cccttctatt
     2881 gaatctatca atggtcaagc aatgaatgat ttgggtttag gtagagatat tgcactgacg
     2941 ttcttagcca tttttgtatt caatattatt ttggctcgtt tcacaaaatg gaagtacatt
     3001 tttttaactg gtcaagcaat tttatggatg gcaacgatga cgacagtctt tggctatttt
     3061 gccggtctgc gagggatcgt attgatcttg gtcggtggtt ttatcggagc ttgtttcgcc
     3121 attgctatgc cggcagtagc gcaaccgatc attcgtaaaa tcactggatc aaatgatatt
     3181 gctttaggtc atttttgtac gatcgggtac ttatttgaag ccggtgttgc taagcttttt
     3241 ggtgagaaag gcgaaaataa aaagtcgatc gaagatatca agttgccgac acatttcgaa
     3301 tttctgcaag atacttattt gtctgtcatg gtcgtgatgg tcccgctata tatcatcact
     3361 gttttatttg caggcgaacc attcgcttct gaattatcag gagatcaaaa ctatatcatg
     3421 tttgcctttt tacaagccat ccaattcgta gtgggtgtgt atgtattgct agctggtgta
     3481 cgtctactgt taggtgaaat cgtgccggca tttcgaggga tagctatgaa attagttcct
     3541 gatgcgatcc ctgccttaga ttgtccagtt ttctttcctt atagcccaaa tgctgtcatt
     3601 ttaggtttca ttacgacaac gattggtacg attattgcca tgtttacttt gcccatgttc
     3661 ggcttagcga tgattttacc tggtatgcta accaatttct ttgccggagg gacagcaggg
     3721 atatttggca acgcagttgg cggacgccga ggagcgatca ttggcggtat tgctcatggt
     3781 ttctttatta cattattgcc cgctttgcta gtaacgatct ttaattcgat gggattcaac
     3841 aatgctacag caaccgatgt agatacagta gccgcagcac tcttatatgc ctggatctta
     3901 agcccagttt tgaaagcatt ttaattgaaa gtagggaaaa tgatgtttgg attttttaag
     3961 aaaaaagaaa aagaaccgaa aaaagttctg gcaaaagaag aaaaagccct atcgccagaa
     4021 gcgaccgaaa agattcaatc agagattaca caattgaaac aagagatttc cactacccaa
     4081 gataagcata aattagcgaa actttatgaa caagttggac ttaaattttc tgaattgtat
     4141 gtgaacgatc aagccatcca atatttagaa aagagtttgg aaaataaaca gacgatcgga
     4201 gatgggtata aaaaactgat gagcctctat aaccagaagc gtgcagatgc tgctcgtgct
     4261 ggtgatgatc aagggatcga ttactacatg ggcaaaatgg atgagatgcg acaaattgca
     4321 aaacaagtaa cgatcaaagg aaacaaataa aaagataagg tggcaataaa agatgtatac
     4381 aacattgaaa gaagtaacaa aaacagcaga agagttgaat tttacagttg gagcttttaa
     4441 tacgcataac ctagaaatgc ttcctgctat gctaggcgca gcaaaagaaa tgggttcgcc
     4501 gatcattatt caaacaagtg tcgataccgc taaatatatc ggcatgcatg tgttagtcaa
     4561 tgtaatgaca gctattgcag aagaagaaat ggttgatgca gtcttgcact tagaccacgc
     4621 ccgagacttt gatgatatca aagaagcaat cgacagcgga tatacttctg tcatgtatga
     4681 tgggtcccat ctgcctttca aagaaaatat cctgaagaca aaagcagtag tagaatatgc
     4741 ccatgcgcac ggtgtttccg tagaaggaga acttggaaca atcggcggta cagaagaagg
     4801 aatccatgtc gctgaaaatg acaaggtata tacaagaccg gaagacgcag tgaaatttgt
     4861 agaagctaca ggcgtggatg cattagctat tgctatcggt acgaatcatg gacaattcaa
     4921 atcaaaaaca gaagtaaaca ttcccttgct gaaagaaatc gatgcagtag ttgatgtccc
     4981 cctagttatc catggaggaa caggtgtgaa agaagaagat tatcctgaat tgatcaataa
     5041 cgggatccgc aaattcaatg ttggaacgga gcttttagtc aactggacga aagtagctaa
     5101 agaaaccttt agtgaaacag aaatcaacaa gtctcttcgc cataatgtga ttccagcaaa
     5161 tgaagcagta aaagaaattg tcaagcataa gattggactg tttatgaact tggaaagtcc
     5221 aattaactta ggaaataaat gagatgaaaa agtatttcat tcttctggct ggtagtcctg
     5281 gtacaggaaa aacatatctt atgaataaac tacgggaacg atttccagat atgtatgcca
     5341 tcacgctcga tgaaatcaag gaatactatg cagaatccat cgggtttaac aatttggaag
     5401 aacgggcgga acaagaaagg acgaaagttt atcctttcta ttacaaagca cttgatttgt
     5461 atatggaagc aggaaagaaa gtgattgtgt ccgagtatcc ttttagtgat aagcaaaaag
     5521 cacgactcca agaattagca gatagatacg gatatcaggt gatcacgatt cgcctaacag
     5581 cagactttga agtgttatgg caacgtcggt acaaacgtga tcgtgaatca gatcgtcatt
     5641 tgagctatat catggatcat tatcactatg gtgacgtatt ggaagatcga acgaaaggga
     5701 ccaatcatat tacgaaagaa gcattcaaaa agatcatcgc cgaccgaaaa tatgacaaat
     5761 tccagttagg aacactatat gaatttgatg taacagacta tactcaagtg gattacactc
     5821 cactattgga taaattagaa aaagaaatca atgacaataa gtaacaaaga aatggcttaa
     5881 tatcgaaaaa ttggcttttg aacgctgttt accgagaggt cgtgagcctg tcgttcagct
     5941 ccgagtcaca aggagcaatt tcaaaaaaac agctt
//
LOCUS       DBNIBO010000139         5923 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.144, whole genome shotgun sequence.
ACCESSION   DBNIBO010000139 DBNIBO010000000
VERSION     DBNIBO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.144"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(74..1168)
                     /locus_tag="AC1Y77_002317"
     CDS             complement(74..1168)
                     /locus_tag="AC1Y77_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIQ1575864.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="AC1Y77_002318"
     CDS             1357..2118
                     /locus_tag="AC1Y77_002318"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIQ1575865.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="AC1Y77_002319"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="AC1Y77_002319"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIQ1575866.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2893..3648
                     /gene="fabI"
                     /locus_tag="AC1Y77_002320"
     CDS             2893..3648
                     /gene="fabI"
                     /locus_tag="AC1Y77_002320"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIQ1575867.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3758..4810)
                     /locus_tag="AC1Y77_002321"
     CDS             complement(3758..4810)
                     /locus_tag="AC1Y77_002321"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIQ1575868.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4936..5856
                     /locus_tag="AC1Y77_002322"
     CDS             4936..5856
                     /locus_tag="AC1Y77_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIQ1575869.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1179 c         1063 g         1875 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt tgacaatcaa aacattttga ctaataatta agaaatcaag
     2821 aagttatttt agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa
     2881 aggagagtca ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac
     2941 aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc
     3001 tatacttacc aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca
     3061 ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc
     3121 ggtgaatatg ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa
     3181 gaattgtccg gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc
     3241 agcagctact ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc
     3301 gggatcgtta gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg
     3361 ggtattgcga aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct
     3421 aaaggaattc gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt
     3481 gttaaagact atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt
     3541 gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga
     3601 atcatcggtg atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca
     3661 ttctttaaag taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt
     3721 tttcttttta cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc
     3781 cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg
     3841 ttactggtcc tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc
     3901 caccgattgg agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta
     3961 gatcatcaat gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact
     4021 gcaacaagga atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt
     4081 ttccgtcttt tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg
     4141 tggctggatc aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa
     4201 gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac
     4261 gatcgtaatc agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta
     4321 ctggcgtaca gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac
     4381 cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa
     4441 aatcttcatt tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca
     4501 ttggcatcag caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa
     4561 agagattgac cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac
     4621 cataatgaag cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc
     4681 cttcttccca ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag
     4741 aaaaacctag attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac
     4801 attttatcat tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt
     4861 tatttttgct atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt
     4921 agaaaagagg attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc
     4981 gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg
     5041 attgattggg acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg
     5101 atcgtccttt tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat
     5161 cagtattcta aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta
     5221 aatactattt ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc
     5281 ggtgtgccga tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt
     5341 ggtgcccaag ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag
     5401 atggatgttg gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct
     5461 gttggtatcc gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat
     5521 cgaaacatca ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt
     5581 cggattgttc ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt
     5641 acattagctg aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg
     5701 gatcttggaa acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa
     5761 tatgccttaa aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc
     5821 acgattccga acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc
     5881 tcactaaaaa gagcgtggga caaaagtaaa gaacactttt gtc
//
LOCUS       DBNIBO010000140         5899 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.145, whole genome shotgun sequence.
ACCESSION   DBNIBO010000140 DBNIBO010000000
VERSION     DBNIBO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.145"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            57..863
                     /locus_tag="AC1Y77_002323"
     CDS             57..863
                     /locus_tag="AC1Y77_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1575870.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            875..2095
                     /locus_tag="AC1Y77_002324"
     CDS             875..2095
                     /locus_tag="AC1Y77_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575871.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2085..3671
                     /locus_tag="AC1Y77_002325"
     CDS             2085..3671
                     /locus_tag="AC1Y77_002325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1575872.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAVGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3710..5848
                     /locus_tag="AC1Y77_002326"
     CDS             3710..5848
                     /locus_tag="AC1Y77_002326"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1575873.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         1990 a          953 c         1239 g         1717 t
ORIGIN      
        1 atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt
       61 ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta ctgctacagt
      121 tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt
      181 ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag
      241 cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct
      301 ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt
      361 tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc
      421 tgtacaccca tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat
      481 atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga
      541 caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa
      601 ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt
      661 ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt
      721 atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt
      781 tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac
      841 cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa
      901 agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct
      961 caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga
     1021 aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat
     1081 gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa
     1141 aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa
     1201 tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga
     1261 tgatatcatt gaatttgctg acatcggcga ccatatttat caacctgtaa aaacctattc
     1321 aagtgggatg ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt
     1381 gattgtggat gaagcattgg cagtaggaga tcttgaattt caactaaaat gtatggaaaa
     1441 atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg atattagttc
     1501 gattcgccgt ttttgtgatc gttcctattg gttgaaaaat ggaaaagttg tcgaatatgg
     1561 tgatacgatg gaagtaacga cgaattacga aaactttttg aagaaaaaat cagttaaaac
     1621 agtagatcga aataaaaacc aagtagaacg cttcgcagct ccagatatcg tagacgtgac
     1681 gaaagccgaa ttgttggata aagataaaaa tccagtggat atgctggaac aaggagaaaa
     1741 actatacgtt aaagtaacct atgaagtaaa agacgataca atcaaaaatc cagttttagg
     1801 aattgctctg cgtacagtcg acaattttta tgtgtgcgga ctcaatactc tactagatgg
     1861 tatcaagatc ccttggaaaa aaggtgagaa tatcttttat ctggagtatg aaaagttagg
     1921 tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc
     1981 tttggtatac aagacaaaat acatgaactt gtttgtcagc ggttcttata tcggagaagg
     2041 catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg
     2101 aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa
     2161 gtaaagtttt agagtttggt cctggaaacg gccgattaac gaaacatttg atcggagcaa
     2221 aacaatgtga agtgagcatt gtcgagctag ataaggaatt gttcgatttt gtcagcgagt
     2281 ttgcccaaga tggattttat ggagatatcg aaagttttga atgggcaaac tattatgctg
     2341 gacaaacatt tgattacgtt ttgtttgccg atgttttaga acatttggta gatcctggaa
     2401 aaacgttgaa gaaagttaga gaatttttga atgaagaagg agaaattcta attacattcc
     2461 ctaaccttgc gcataattct gtcatgattg atttgttcaa taatcaattg ccatgggctt
     2521 cttatggttt gctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag
     2581 tttttgaaaa agcaggatta tacatcaata ttgaagatta tttgtactta gctgtaggtg
     2641 atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgttat gatttcaaaa
     2701 tgcgaccatt tggcgaagtc taccaatact tcttctcatt gatgaaacat ccagtagcac
     2761 aaagttccat tgcgcagccg caaaattcta attatgtgaa agtattagaa gtaatccagc
     2821 aaacgaaaca agatgaaata attcaacaaa taccattcaa taatttcaca ggggaaaatg
     2881 agacactaag tttccatgta tcagaattag cagaacgaat ggtgtttcgc ttcgcgcagc
     2941 aacctagctt tatcgaattc agcgcagaag ctgttggaga aaaactcaca tttatcgatt
     3001 caaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatggtaaa gatttaccag
     3061 agttcttttt aacggatatt gcagggaaag aagtgactat ccattgtcac tatcgtttta
     3121 tcggagaatt gacaccaaca atgaaagaat tgctggaaac aatccgtcca atggctgaag
     3181 ttacaaagca gctttctgag aaaaatgatg aattagaaag agaaaatcat tatcttattg
     3241 agaataatac aagattagat cagacgttga aaacaaccat aaatcgctat tgcacgttgt
     3301 tggaaagtga tgaatggaca atcaaacata gattagggcg acgtaagaaa gaaacatcga
     3361 aaaaaatcca agaaaaagag ctttctctat gtattgacga aaaaatctgg gatgcagaaa
     3421 caaagatatt gaaaatcatc gggtggggaa tcgctaattc ggatcgccaa ccattgtcct
     3481 ataagttatc agccgaccaa tcaccatttt ttgaagcaat tcctgtttta cgagaagaag
     3541 tcaatcaagc aaaacagcta gctgaaggag caaaagcagg atttgaactg cggatccttt
     3601 gcgagaagga acgttcattc ttagttgaag cagtggctca aaatggtcaa tcatggatca
     3661 ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa
     3721 agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat
     3781 tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga
     3841 aaatgtgatt gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct
     3901 tgatgttaag acccaaagcg gttttaaggt acgtttatca gggaaaatga gaggaaaagc
     3961 aatcttagac ttccaaacag caagacatca aaatggcata gctgtgaaat taaacggtaa
     4021 atatccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa
     4081 agggataaat tatgcaagga ctcatggcgt aaaaaaagca atccgccggg tcaaactgga
     4141 attgaaacca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aacctgatct
     4201 gaaagagcaa aggaaagaag tacaaggatt tgcctaccgc ccattgattt ccatacttat
     4261 gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tacttgatca
     4321 aacgtatgat cactgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg
     4381 aaagtgtcta gaatcctatc aagcaaaaga tgatagaatt aaagtcgttt tccgacaaga
     4441 aaatggacac atcagtctgg caactaattc tgcattggaa atggcagaag gagagtttat
     4501 agcgctctta gataatgatg acgaattacc tccatttgcc ttatatgaag tggcaaaagt
     4561 gctaaacgta catccagaac tggatttgat ctacagtgac gaagataaaa tcgatgcaga
     4621 tgggaatcgc tttgacccgc acttcaaagc agactggtct cccgatacat tgatgggaaa
     4681 taattatatc tctcacttag gcgtatatcg gacaagcatt gttaaggaac ttggcggttt
     4741 ccgtaaagga tatgaaggct ctcaagatta tgatttggtt cttcgtgtga cagaacaaat
     4801 tcctgcagaa cacatttacc acattgaccg tgtcttgtat cattggcgga cgatcccggg
     4861 atcaactgca agcaatggag aagctaaaag ctacatctat gattctggag tgaaagcatt
     4921 gacagatgct ctatctagaa gaaatatcaa gggaagcgta catcctggca gaatttctgg
     4981 tttttatgaa atcacttatg atgttttgca agaagacttg gtcagtgtga tcattccaac
     5041 taagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta
     5101 tccaaattat gaaatcatta ttgcagataa tggaagtaca gatcctaaaa tgcaagaatt
     5161 atttgcagaa tacaagcatc agctgaaaga ccgatttatc gtcgaattaa ttgacattcc
     5221 attcaattat tcacgtatca acaatctggc agcagaaaaa gcaaacggca aatacttttt
     5281 attcttgaat aacgatacag aagtgattga acctgactgg atgacagcga tggtttctta
     5341 tgcacagttt gatcgaatcg gctgtgtagg agcaaaatta ttttatccgg atgatacaac
     5401 gcagcatgca ggtgtattat tagggatcgg tggcgttgca ggacatgcat tgaataacta
     5461 tgatcgaaca cattgtggtt actttggccg attggtcatt gacgtcaatt acttagcagt
     5521 cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgtcaatg gatttgatga
     5581 aatattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg
     5641 ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta
     5701 tgaagataca ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa
     5761 atggccagca tatattgcgc atgatccatt ttacaatgat aatttaacaa aagaaggaat
     5821 cggcgacttt tcgttgagac cagattaaac tataataaaa agctgttatg ttgtgattcg
     5881 ctcgcacata acggctttt
//
LOCUS       DBNIBO010000141         5851 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.146, whole genome shotgun sequence.
ACCESSION   DBNIBO010000141 DBNIBO010000000
VERSION     DBNIBO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.146"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            214..999
                     /locus_tag="AC1Y77_002327"
     CDS             214..999
                     /locus_tag="AC1Y77_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIQ1575874.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1012..1863
                     /locus_tag="AC1Y77_002328"
     CDS             1012..1863
                     /locus_tag="AC1Y77_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HIQ1575875.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1987..2439)
                     /locus_tag="AC1Y77_002329"
     CDS             complement(1987..2439)
                     /locus_tag="AC1Y77_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575876.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(2904..3272)
                     /locus_tag="AC1Y77_002330"
     CDS             complement(2904..3272)
                     /locus_tag="AC1Y77_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIQ1575877.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3422..3904
                     /locus_tag="AC1Y77_002331"
     CDS             3422..3904
                     /locus_tag="AC1Y77_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIQ1575878.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            3941..4951
                     /locus_tag="AC1Y77_002332"
     CDS             3941..4951
                     /locus_tag="AC1Y77_002332"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIQ1575879.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5066..5779)
                     /locus_tag="AC1Y77_002333"
     CDS             complement(5066..5779)
                     /locus_tag="AC1Y77_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575880.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1922 a          958 c         1167 g         1804 t
ORIGIN      
        1 aatttttgta aattacttct tatttcttga agctggccac ttctgtcaca acctctttct
       61 ttatagaaat aaaaggacac tagcaattca tgaaaacgat tgctaaagcg tatcatttgc
      121 tttagactat atatcttggt gtacactcac tttaagacgg aaataaaagt tatccgtaat
      181 aacaggttta ggtatgaaaa aggagtgggt actatgtata aggaattaga aaacaaagtc
      241 gcagtagtaa ctggtggctc aaaggggatt ggaacagcga ttgctcaacg attcgggaaa
      301 gaaaaaatga atgtagttgt caactataac tcggataaag aaggagcaga aaaagcagtc
      361 aaagcaatcg aagaggcagg cggaaaagca gtctctgtac aagcagatgt cagttctgaa
      421 gaaggaatac agcgtttact tgatacggct ttagaaaatt ttgggacact ggacgtttgg
      481 attaataatg ctgggatgga aaatcagcat ccaacccatg aacttagcct tgaagattgg
      541 gaaagagtat taaaagtcaa tttgactggc gtttttatcg gaacgaaagc ggctttaaat
      601 tactttacag agaatgataa aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa
      661 atcccatggc caacgtttgc tcattacgct gcttcaaaag gcggagtaaa actattcacc
      721 gaaacggtag caatggagta cgcaccaaag ggaatcagaa tcaatagtat aggacctggt
      781 gctatccgta cacctattaa tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg
      841 gaaagtatga tcccactagg acgcgtaggt gaaccaaaag aaatagcggc gacagctgca
      901 tggttggctt cagacgaatc cagttatgtt acaggtatta cactatttgc agatggtggg
      961 atgactttat atccttcttt ccaaggtgga aaaggataag ggtgaataaa aatgggttta
     1021 ttgattgcat tgatcccggc agttgggtgg gggatccagc cactgatttt gaaaaagatt
     1081 ggtggcagac caacaaatga gatattagga actggtattg gagccgtgct cgttggctta
     1141 cttgttcaat tattcgcttc tccagctggt atttcagtga ctacattttt gattagttta
     1201 ctttctggtg cattctgggt catcggacaa acaggacagt atacatcttt caacttgatt
     1261 ggtgtttcta aaacgatgcc aatttcgact gcattacagt tagtcggaac ctcattgatt
     1321 ggtgtcttcg cttttggaga atggtctgga agtatgggaa aaatcattgg tgcagcagca
     1381 atcattcttc ttgtatttgg ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa
     1441 ggcggtatca caaaaggaat cagtctatta gcatcaacaa gtattggtta ttgggtttac
     1501 agtgcattgc ccaaactagt tgatgcaagc ggactttcca ttttctttcc gcaaatgatc
     1561 ggtgtctttt taggggcagt gatttatgtg attttccgaa aaccaaccgc attttctgaa
     1621 gacaagagtt ggaaagcaac aatagttgga gccatcttca gtgtgtcagc attggcttat
     1681 attttttcag caaatgaaaa tggggttgct actgcatata tcattacaca gctaaacgta
     1741 gttatctcta cattaggcgg tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa
     1801 aaattaacga ttatcggatt attgctgatc gtagtaggaa gcatggtcac ggtctttatt
     1861 tgaggataaa gatggtaaaa tctcatttat taaaaaaata tttgtttgaa ccaaacagta
     1921 agtacttata aaaaaatagc gctagaattt ccaacatctc tggaatttct agcgcttttt
     1981 aattgtttaa gatacaagtg aaatatctgt ctgcatcaat aatttttcat tgatccattg
     2041 actaaaattt acgacatgat tgaagttacc accgattagt atctccgcat gattgtcttt
     2101 ataaagatcg tattgtaaaa gacggtcttt tttatttgcg taaacatcgt tatggtactg
     2161 gactaattgt tctcggattt gattgattgc tgtaaaatca cgatactgaa ggttgaaacg
     2221 catcgtgatt ggtgcattta gcatgaatcg ctgcatttct tttgctgctt cctctgcttg
     2281 atctccgtat aggaactcaa aaagattacg ttcagaaaat ttccattgcc cgccaacctt
     2341 tgttcctttt agttttcctt catgaagata atttctgata gaacgtgtag taacacctaa
     2401 ttgttcggct aattcattta ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat
     2461 ggttctactg acttagttga tatttgctta atcacgaaat gtagaaagaa cgattttaat
     2521 taaggtcatt gtaaaaatca agtcagaaaa ttctaacttt tctttctcta aagaaattcg
     2581 ttctctccta aagtgatatt atatttaaat atacctgaaa ataatgggca gtattatgaa
     2641 agatcactga tattttctcg tattttttcc ttattcaaaa gttttgcagg aaaattgctg
     2701 aaaaaataat tttactaagt ggaaaattca aagccattca atcagtgatt tttttcagca
     2761 caagaaagac tccttataaa aatagcagat actttgtcaa ttaagtgttt aaccagggat
     2821 gataaaaagg ataggattga acaaaacctg tataagctga tgaatagttt cctcgccagt
     2881 aaaaggttta ttcgtataaa gctttatcgc caaaatttat catgaccttt tttatcttca
     2941 atattaaatg aaatcaattg ttcaatcaat tcccagttga ttacactttt ccaaggaact
     3001 ttgatgacgt tatccgtata tacgtagcct gcttcttgga tatttttttc aagggcacga
     3061 atcgctgctt tttccggtgc gatagaaaaa tgatttttag caaaactaaa tgcaataata
     3121 aaggtaccat ggtcagtaaa cataggttgg ttccatttta cagttgtgtc tagctgggga
     3181 tacgtttctg ttatcttagc aaaaaggtct ttgatctttt gttgatgttc tttttctttg
     3241 atctgattga tgatcggctg aaatttttcc attctaaaac gcctccatta agtggtttga
     3301 tttattttac cagaaaaaca atagttatgt ataaaaaacg aacaaaacac aagtaatgtt
     3361 gtaaccgcta tatttatatg ataagatgtc tcaaataata aaatagaaaa gagacataaa
     3421 aatgattgac ttggcaaaga aattcttaca cgaaaatata tattttgagt tgtatcatca
     3481 tcgagccatt tatacaaatg aagatgcttt ggtaataaag aaagaacaag gatttacagg
     3541 tacagagacc aaaagcttat tcttaaaaga taaaaaagat aataactacg tatatctgac
     3601 ttttactacg aaacaaacgg atttcaaagc attagggaag atcgtcggta aaaggctttc
     3661 tattgtttca tcagaacgca tggaagaaca aacgggacaa aagcagggcg ctgtttctcc
     3721 gtttggttat gaaaaacaga ttccgattat tattgatgaa gagctcttat caaatgaaaa
     3781 acttgttttt gcaccaggta gaccagatca aacgatggtc atccaaacgg ttgacttaaa
     3841 caaaatcatc cagctacttg agatgccgat atttttgatg ccggcaaacg aaaaaaacaa
     3901 ttaaccaagt aaaatagtgc atacttggag gtgaagcaac atgataagtt atccattatt
     3961 agaaagaaat gcaacgattg gggttactgc accatcctca ggcgtatcag aggaacacca
     4021 ttcgttactc gaatcagctt taaagagaat ggaagaaaaa tctttccaag tagttactgg
     4081 aaagacgctt tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac gagcagatga
     4141 attcaatcaa atgatgcaag atgaaaatat ccaaatgatc tttccgcctt ggggaggaga
     4201 attattgatt gagattctcg attttgtcga ttttgatgct ctacaaccaa aatggatagt
     4261 aggttattca gatattagta cgttgctact tgctgtcaca ttaaaaacag ggatagccac
     4321 tgcacatgga accaacctta ttgatttaag aggagagtac ttggatgaaa cgacagcaat
     4381 gtgggaaagt gtactgaaaa caagagaagg agaatcgatt ctacagcaac cttctgaaaa
     4441 gtatcaaaaa gagtggcccc atgagcattc atcatcttct gttttccatc tcactgaacc
     4501 aacaaattgg caatcagtca gcggagatgc agtaaggatg aagggacgat tattaggtgg
     4561 ctgtattgat acgatcagtc atttaatcgg taccccttat ggagatgtcc aatcttttaa
     4621 aagaaagtat aatgatggaa agccgatctt gtggtttcta gaaaattgtg agctatcgtc
     4681 agttgattta agaagatcac ttgttcagat gaaactggca ggatggtttg atggcagctc
     4741 ggggatcatg ttcggtagaa gtgcagcaga ggatttgtta gattataaaa tgaaggatgt
     4801 atatgaagat ctgtcaaagg aattaaaact accgatcttg tatgatattg attgtggtca
     4861 tgtaccgcca caactgacat tgatcaatgg tgcctatgca gaagtagcgg tagaagatag
     4921 aaaagcgact gttttgcaaa cgtttattta aaaaattttt tcctgccttg gagtttatca
     4981 aagaccaaca aatctaaata gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt
     5041 ctttttgtat gatctattag aaattttaat tatccataac tatcacattg atcagctgtt
     5101 tcacatcatt tggattacct gtaggaatag ctactacaga tgcattatca ataatttctt
     5161 gagttgtttc tctaatgaac gctggataag atgtttgatc tgcaggcatt tgccaatgtg
     5221 aaggaactgg ataactagtg attgtcccgt catggttact tgaatacact aaaactccct
     5281 ggtaactgta tttcccagtg agcataacca catctttcgg atagactgca ctccctgcat
     5341 cataaggggc gataggtgtt cctgcaggat aatgaataac atgtagttcg aaatcacccg
     5401 atgtatctct aaaagtatta cctctaattg ctagccaaac acgtgcgtac tcaatttgta
     5461 gatcagaata tcctgctaac ggatctattg actgagaaga ctgtgaggaa gttgtgtcta
     5521 ttgttgaaga agaaatattt gtctcaactg tactactaga tgtagaagaa gtattgggct
     5581 ctgagacttt actgcttgtt gaagatgtat tcgtttcagt agaagctgat ttctgagtac
     5641 ttgatttttc agctgaactg gtttgtttga ttgaactttt gtctgtggtc gcagatggat
     5701 cactattttc tttactagga gacgtacatc ctacaaggaa taaagagacc aacaataaaa
     5761 taaaacctaa ctttttcata taaaatacct cattattttt ttaataccag tatagcataa
     5821 tcctgaattt ttgaatccgt aaaaccttgt c
//
LOCUS       DBNIBO010000142         5820 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.147, whole genome shotgun sequence.
ACCESSION   DBNIBO010000142 DBNIBO010000000
VERSION     DBNIBO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.147"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(6..1207)
                     /locus_tag="AC1Y77_002334"
                     /pseudo
     CDS             complement(6..1207)
                     /locus_tag="AC1Y77_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1324..1839
                     /locus_tag="AC1Y77_002335"
     CDS             1324..1839
                     /locus_tag="AC1Y77_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1575881.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2038..2484)
                     /locus_tag="AC1Y77_002336"
     CDS             complement(2038..2484)
                     /locus_tag="AC1Y77_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIQ1575882.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2531..4009)
                     /gene="msr(C)"
                     /locus_tag="AC1Y77_002337"
     CDS             complement(2531..4009)
                     /gene="msr(C)"
                     /locus_tag="AC1Y77_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HIQ1575883.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4363..4992)
                     /locus_tag="AC1Y77_002338"
     CDS             complement(4363..4992)
                     /locus_tag="AC1Y77_002338"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIQ1575884.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(4976..5440)
                     /locus_tag="AC1Y77_002339"
     CDS             complement(4976..5440)
                     /locus_tag="AC1Y77_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1575885.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT         1825 a         1227 c          972 g         1796 t
ORIGIN      
        1 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
       61 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
      121 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
      181 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
      241 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
      301 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
      361 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
      421 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
      481 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
      541 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
      601 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
      661 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
      721 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
      781 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
      841 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
      901 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc aaaccgatcc
      961 gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa
     1021 ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg
     1081 ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg
     1141 aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata
     1201 tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt aaatatgcac
     1261 atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt
     1321 tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg
     1381 tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca
     1441 acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct
     1501 caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa
     1561 gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg
     1621 acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa
     1681 atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt
     1741 ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt
     1801 cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta
     1861 gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa
     1921 aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca
     1981 ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca
     2041 ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg
     2101 caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg
     2161 atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa
     2221 aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag
     2281 tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac
     2341 cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca
     2401 taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat
     2461 tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat
     2521 cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat
     2581 aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc
     2641 ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag
     2701 tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac
     2761 gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc
     2821 ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa
     2881 aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc
     2941 ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg
     3001 tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat
     3061 caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg
     3121 gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct
     3181 gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt
     3241 ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc ggtctgattt
     3301 gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt
     3361 ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag
     3421 tctttttctc caattccttt tgagtgagat aggcactata attccccgaa aactctcgga
     3481 tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat
     3541 gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac
     3601 tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa
     3661 gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt
     3721 cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga
     3781 gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg
     3841 gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca ttttctccta
     3901 tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt
     3961 tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac
     4021 tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc
     4081 aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa
     4141 atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat
     4201 gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac
     4261 gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga
     4321 ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga acgtagtgat
     4381 cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa
     4441 aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc
     4501 ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg
     4561 catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact
     4621 cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc
     4681 tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca tatcgccaaa
     4741 aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg
     4801 caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt gcatattgac
     4861 agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt
     4921 gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt
     4981 tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat
     5041 tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata
     5101 gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc tgggcttttt
     5161 ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc
     5221 catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt
     5281 cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc
     5341 tagtaagact tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt
     5401 tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa
     5461 ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat
     5521 cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt
     5581 agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt
     5641 atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca aaaatttccg
     5701 aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag
     5761 taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa
//
LOCUS       DBNIBO010000143         5770 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.149, whole genome shotgun sequence.
ACCESSION   DBNIBO010000143 DBNIBO010000000
VERSION     DBNIBO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.149"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            18..1739
                     /locus_tag="AC1Y77_002340"
     CDS             18..1739
                     /locus_tag="AC1Y77_002340"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575886.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            1754..3538
                     /locus_tag="AC1Y77_002341"
     CDS             1754..3538
                     /locus_tag="AC1Y77_002341"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1575887.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3919..5472)
                     /locus_tag="AC1Y77_002342"
     CDS             complement(3919..5472)
                     /locus_tag="AC1Y77_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIQ1575888.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
BASE COUNT         1827 a         1101 c         1102 g         1740 t
ORIGIN      
        1 aaaaaaggag gaataatatg tctatcttaa aaacattctt acagaaaaac aaaggactcg
       61 ctctccttac ctttgtgatg atctgtttag aaatcgccgg tacattaggt gtaccaaaat
      121 tagtagcaaa gctgatcgac acaggtatag caagtggcga agcctcagtt ataaaaaaaa
      181 ttggagttga aatgctgctt gtagcgtttc taggaacaat cgctgcagtt gcctctagtt
      241 acttttcagc attggcttcc gctaggtttg gattccaaac gcgggagaaa tatttccgta
      301 aattccaaca gctgtcaatg aaggatatca gtcgttttag ttctggatct cttttgacaa
      361 gaatgacgaa tgatgtcgat aatgtccagc aaatgattgt tttattttgc caaatgatct
      421 taccagcacc ggttatttgc gttttcacca ttctcatgat gtttcgttat tctctattac
      481 taacatgggt tacattgatc tctgttgttt tttatgtatg gattgtttat cgactgatga
      541 aacgaggggc acctttatct ttgagtatcc agccaaaaat ggatcggatc accatcacat
      601 tgagagaatt ctttactggg attaatatga tccgtgcctt tgataatcaa tcatatgaag
      661 aaaagcgaac gaataagagc tttgctgact atgctaatca aatgatcaaa gtgaaccgaa
      721 tatttgcttg ggtcactcca attgcttttt tgctcatggg aatcgtttat gcttctattt
      781 tgtggtttgg cggaaatctt gtagcagaag gagcattaca aatcggtgtc gtgactgctg
      841 tagtcgaata ctctatgctg acacttgctt acttgatgat cgcagctatg gtattggtca
      901 ttatccctaa atcgtttgcc tcattacgac ggatagaaga aatactgcaa gcagagatcg
      961 aaattgagga agagacgatc aaaacagtag ctgaaccaac ctttgatcag acaaatgcag
     1021 ctgcttttga acatgtcaca tttaaatata ctgaaatctc tgatccagtt ctagaagata
     1081 ttgatttcac aatacctaaa ggaaaaacaa cagcgatcgt tggcgggacc ggatctggaa
     1141 aaagtagttt ggcgaaactt ctcttgagac ttagtgaccc tacgatagga aaagtaaccc
     1201 ttggtggtat tcctcttact caaatgactc aagaacagat ccgctcccac atcagttatg
     1261 tccctcagaa ggctttctta ttcagcggga caattttaag caacttgcag atgggaaatg
     1321 aatatgcctc aacagaagag ttgtctaaag cgatcaagat cgctcaatta gaagaactag
     1381 tggacaccct tcctgacgga ttggacagct ttgttgctca aggcggttct aattattcag
     1441 ggggccagaa acagcggata tgtattgctc gagcgttgat caaaccagca gagatctatg
     1501 tattcgatga tagtttctcc gctttagact ataaaacaga tgcagcatta agagcagccc
     1561 ttcataaaca catgtcggag aaaactctcg tgattgttgc tcaacgcctc agtacaatcc
     1621 aacaagcaga taacatcatt gttttagatg aaggaaaaat cgttggacaa ggtactcatg
     1681 aagaactact gtctaattgc caaacttatc aggagtttgc aaaatcacaa ggaatataga
     1741 aagaagggat acgatggaaa agaaaaaaac gttgaacacg cgtgctttga tacgtttttg
     1801 gaaaatgatc aaacctgaac ataaatattt ttacggattg tttctatgca gtttattcgg
     1861 gaacctatta gtcgtagcca tgcccttgat catgggaatc gggatcgatg atctcttatc
     1921 agaaataaga aaaacaggta ttggacagct ttctttcagt gatatcaaac aagcattgtt
     1981 gttccctgtc ttattgctga tcggctgttc agtcttaagc agcatcactt catttattca
     2041 ggaacggaca atggcagcgt taagcgaaaa gatcacattg agggtgcgaa aagaaatcac
     2101 aaaaaaattc aaaacattac cgatgtcgtt ttttgacaat catcaagtag gtgacatcat
     2161 tagtcggaca actaccggtc tcaatcaact ttctcaagtg ttattgacag gtatcaatca
     2221 attttttact tctgtcgtaa caatagctgc tgctggtctg atgctgtttt atatcgaaag
     2281 caagcttact ttgctcgtct tattgctgat cgttggcagt tcaattgtta caactaagat
     2341 tgcgaacaaa aacaaacaga tttctgaaca gaatcagtat gaactgggtg ttttaaataa
     2401 tcgaatggaa gaaatcttag ctggtaatct agtggtcaaa acattcaatc agcaagctca
     2461 tgcagaagct gctatacacg aagtaaataa aaagcaatac aacgctttta aacgtgctca
     2521 atttatgaac tgtgctatct atccggccat tcgtttcatt aatcaactcg ccttcatcat
     2581 aagtgcggta ttcggtgcta tgcttgttct atcaggtggt gtgacgatcg gcttcttaca
     2641 agcttatctt cagtacatca atcagatttc tgaaccaatt tctaccgctt cttatgtgat
     2701 caactcgatc caatccgcaa tggcagcaat cgataggata tttgagatat tagatgaaac
     2761 agatgaaatt ccagaccttc cagatgcaag acttttagaa caaccgaaag gaaaaatagc
     2821 tttcaaaaat gtacagtttg gttacacatc agataaaata ttgatgaatc aagtcgattt
     2881 ttcagtaaag ccaaaacaaa cggtagcgat cgttggtcca actggtgcag gaaaaacgac
     2941 tttagtcaat ctgctgatgc gtttttatga actgaataaa ggaaagatcg aattcgacgg
     3001 tcttgatatt accaagcttt cacgtggaga actacgtcgt tcttttggga tggttttaca
     3061 aaatacctgg ctgtttgaag gaacggtagc tgataatatc gcctacggga aaatggatgc
     3121 cacaagagaa caaattatcg aagcggctga aatcgcacaa tgtgatcact ttattcgaac
     3181 actgccagac ggatatgata cgatcatttc tagcgaaaac ggcacgttat ctcaaggaca
     3241 gcaacaatta ttgacaattg ctcgagtcat tctagcgaat cctcctgtcg ttattttaga
     3301 tgaagcaact tctagcgtag atacacgaac agaagcattg attcagcaag caatggaagc
     3361 tgtgacaaaa gataggacaa gttttgtgat tgcacaccgt ttgtcaacga ttgaaaatgc
     3421 agacatgatc cttgtaatga agaatggcga tatcgtcgaa aaaggaacac attctgaact
     3481 actccaatca ccaacgcttt atgccagtct ttataatagt caatttcaga tagcgtaaca
     3541 aacattatct aaaaagctat cgatcatgta tgattagtcc atgatcgata gcttttttaa
     3601 atgtactagt ggtgccacta gaaacacaaa gaataatact aatgatactt tttggatagc
     3661 tccttcagtt tcgttcggta agtagtgaca aaagacttgt cacaatccct ttttacgaat
     3721 aaacggtgtt caagagacaa cctatcggca ataagacacg atcttgtaaa atacgagaag
     3781 ctatacttca aaatcgcttc ttgttgctct tgtcaactcc gaatcacgag ttacactatt
     3841 caacaaatat gagaagctgt tttttgaatt tgtttcttgt gactcggtgc tgaacgtcag
     3901 gctcacaatc tctcttcttt aatctccttg ttctacttgc gatagttgct gaacgaattg
     3961 ccatgtttcc ccgttatcag ttgaacggaa gaggcaggca aacattttcc ctgcatcgat
     4021 tggtcctttt ttcaacaaat aaaattttgt ctctaacgta ttcgcgctta tttgagtaac
     4081 ttcatttggt gattgaaaaa gatctatccc ccctgtctga tagttttccg gaatcgacag
     4141 aactgattct ttgaatgagc gtcctccgtt gtttgtataa aacaaacttt cacgtgtcga
     4201 aacaaaacct aaagaagttg taacgaagct gacattttgt actggctgat tgatcattgt
     4261 acttccactt tttatccaag tgattccgcc atccatagta gtgtaaatgt acaaagtttc
     4321 tgcagacatc ccactggctg atgtacagat aaatagacca tcgttgccat aaaataccgc
     4381 ttttcgataa cgtatcggct gaactgattg ggtgataata gacgtttgcc atgttttacc
     4441 attatctctt gaagtcagca gacgtacatc atccccagaa gaatagataa accatgagaa
     4501 atcggcggaa atatcatatg tgttgtccat ccaataacct tcaggaatca gccccgttgt
     4561 cagggtgtat tcgccaaaac gcaaccattg cgaatcaatc ggcacaaata cccaattagc
     4621 accactatct gttgtcatat agagagagtg cgtctctgta tcaagtttga gatcatttat
     4681 cttttgtgtt gtcaaattta ccttttgctt aggatcagct aacaaccctt catttgtctt
     4741 ttgtatatgc gtatcgttag ttgagttcat tgtcatgtga gaagcagcat ttatatgaga
     4801 agacattttt ggctgagtgt atctcccata atagattgtt ccagctaaaa gaatgaggaa
     4861 aaaaccagtg actaatgtac tcatcttagt tgtcaggtat ccaccaggcg aagactggct
     4921 tttttcccat ctgggttcgt tttctagtaa aaaaatatgg aatgtgttca ttccgaattt
     4981 tgatttgctt tcgcctaagt agataggtaa atcatcaagt gatctcagca ataactggca
     5041 atcacttttt tgtatccgaa aagcttgact cgtcgaagtc ttctcaaaat aaagatagtc
     5101 atcatcttga gaagttggtt tattatgtaa aaactgttgt gctctttgac taagccattg
     5161 atcgaaatga cgcgacaatt gatggtaacg gagccaaata agtagaagaa aaacaataag
     5221 cacaacaatt gttattccgc caactcccca cgaaaagtga tacaaattat ttaatttttg
     5281 catcccttca gctgaacgga ctactgcact catatggcga tataggaacg atacagctcc
     5341 gaaaaaaagc aaaccagaga aaaatactaa gcttaaaagc ttgaacatta ttcttttcgc
     5401 tctttttttc gctttctcga taattttttg tttagctgat aatacttcga taataaatag
     5461 ttcttgatcc atttgtctct ccttttctca tacttcattc attatgcgtc aaaatccata
     5521 caatcactag tcccactaat aaaaaattta tgaatttggt atgtactctc aatttcaggt
     5581 agtttataca tcaaaaaata ctaagattaa tagacaaaaa tttgatgatt acagagatct
     5641 cttacaagac cattcagaag aaaaagacat tgaacgtgtt tgagaacatg aaaatcaaaa
     5701 cctttttgat caatcaatta atcaatttga aataacaaaa aatctgagac agaatgaagt
     5761 tcattctggc
//
LOCUS       DBNIBO010000144         5726 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.150, whole genome shotgun sequence.
ACCESSION   DBNIBO010000144 DBNIBO010000000
VERSION     DBNIBO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.150"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            195..920
                     /locus_tag="AC1Y77_002343"
     CDS             195..920
                     /locus_tag="AC1Y77_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575889.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            910..1419
                     /locus_tag="AC1Y77_002344"
     CDS             910..1419
                     /locus_tag="AC1Y77_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575890.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1489..2937)
                     /locus_tag="AC1Y77_002345"
     CDS             complement(1489..2937)
                     /locus_tag="AC1Y77_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1575891.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2937..3653)
                     /locus_tag="AC1Y77_002346"
     CDS             complement(2937..3653)
                     /locus_tag="AC1Y77_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIQ1575892.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3634..3984)
                     /locus_tag="AC1Y77_002347"
     CDS             complement(3634..3984)
                     /locus_tag="AC1Y77_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIQ1575893.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4128..4901
                     /locus_tag="AC1Y77_002348"
     CDS             4128..4901
                     /locus_tag="AC1Y77_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIQ1575894.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5072..5438
                     /gene="ssrA"
                     /locus_tag="AC1Y77_002349"
     tmRNA           5072..5438
                     /gene="ssrA"
                     /locus_tag="AC1Y77_002349"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1661 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaattattgt cgctgctcct cttttcgaat aaacagtgtt caaaagcttg catctacggt
       61 aatagaatag ggcagcttct tcacaacctc ttgcttttta caaaatattt tctggtttta
      121 attcgtgatt tcactaaagg aatttcatac tttattcata ttcattttct atacttttta
      181 tagaggtgaa aagtatgaaa ataatccgct atgctactgc tcgattaatt tattacaaaa
      241 agcagacaat actctattgt ttattttcaa gcttttctgc ttttttattg atagcatgcc
      301 tgactcttta ccattcgcaa tcggctttag ccaatcaagt ccaagatcgg ctttcattta
      361 ttggcgcttc tgcatctaca gagcttcttc cggctttaga attagctagc cgattttata
      421 taaaaggcat attcagttta ttttttctat ttgccttcct ttttatcttt ttcttttact
      481 attcagtcag gcaaaacaag caggaattga tcaattggcg cctgactggt ctttctaaaa
      541 ccaaattatt cctttttatt ttctggcagc tgttctttcc tctctttctt tgttgtatct
      601 tggttttatt gtggatcgtc agcttccagt cagtttatga agcgcttctt caaaggatca
      661 attttcttgt cttaaagact actgaactgc cagatatccg tgatatcttg acttcggtaa
      721 gcggacttac aattccgatg gatcaacaga ctttttttac catcgatttc caaaatgatc
      781 tttttttcac agatatactt aagggatttg cccaaactct tattgggctc agtggatgct
      841 gtctcattct tagtctattc cctttcactt tttttatcca tcatttgcaa agtaggaggc
      901 ctggacgtaa tgaaacctga atatttgatt catgctgaac acttgaccgt tattttgtgt
      961 cctctcgaaa aacagcataa aatattggag cacttgaaaa aagaggttct tgttgcaaaa
     1021 ctggataatt ttgcgatcat ccaaaaaaat tggccgatgt ttccttatct gaatcttaaa
     1081 gaccatgtcc tgttggatgt accggaaaaa gaaatcaaac aagatcgttt ggcctatcaa
     1141 gaaaaacttg atatctctcc ttccttgctt aattgtgcgg cagctgagtt gacgtctttt
     1201 gaaaaagtaa agctgcaatt acttcatgcc ttgctttcca agcgaaacaa tattgttatt
     1261 gaagatacat ttgatgaact tagtgtattg gagatccaag agctactcca cttgctttct
     1321 tatttagctc atgaagaaaa tcaaggtatc ttactcttta ctcatgatgc tactattgct
     1381 caaagtcctt atattgatcg tttagagccc gcgggctaaa tcaatcattt tatagtaaaa
     1441 acaagcggta tagaagatcg attcatgatc ttttataccg cttgtttttt actctttttt
     1501 gatttctacg tcaggcaaac gaacagcgaa ttttgttcct tttcctagtt cgctttctac
     1561 ttctaatttt cctttatgca agcgtacaag ttgctgcacg attggtaaac ctaatcctga
     1621 ttcaccatat tttgtatttt ttcttgaagg atctacttta taataacgat cccagatact
     1681 tttcatttgt tgttcagaca ttccaattcc cgtatcttca atcgtgatga tcgtttcaag
     1741 atagcctttt tctaaagtaa tcgtgatttg gccgttttca gtaaactgta tcgcgttttg
     1801 aataatattg accatgattt ggacaaatcg gtcataatca gcgtaaacat caatatcatc
     1861 aacggctttc aatgtcaatg tatctcctgc tgcttctgct ttagcttcta actgtgcaac
     1921 aatatttttc aatgtttctg ttccattgaa ttttttaatg accatactga tttgattcgt
     1981 acggattttt tcataatcca agttttcatt caccaaacgg atcagacgct ctgtttcatt
     2041 tttcatcaat ttaatcgcgt tttctcgctg attttcaggg attgcattgt attctaaccc
     2101 ttccagaagt ccgttgattg tcgtaagagg tgttctcatc tcgtgagaag catctgccat
     2161 aaattgtctt cgacgatttt cttgttcttc aatttctgct tgagattctt tcaatgaatt
     2221 cgtcatttta ttgaaatcat ctgctaactc atcaaattca tctttgtcat gtacggggag
     2281 ctgtacatca aaattaccat tcgtcacttc tttcgtcgca ttccttaacc gattgatacg
     2341 cttaacttgg aatgctgcaa aagcatagct ggcaataact gcaacgatac tggaaaaaat
     2401 aaatcctttg aacaggttca gtgtaattgg acgaacgctg ttatcgatat ttcgcgcagg
     2461 ttgactaact acaagtgctc catagaattc atgattcaaa ttaaatggca caagtgcgta
     2521 agaggtcgcc tgtgcttgtc ctaaaatatt tttattcgat gtgaattttt cttgacgtcc
     2581 attcctcaga tttttccatt gatcagctgt taaggtaaaa ttcagtaaag ctgtattagt
     2641 cggataaatg acacgttcgt ttttatcgat aaatataaag ttcacatttt gttctgtcag
     2701 tacttgctct gtcagcagca atgcattttg aaacgcctga tcttgactaa actgcgggag
     2761 cgtatcggca taggtttgcg ttgtcttttc aactgattca gcatatccaa acagttgctg
     2821 gtaattgttt tcttcaatcg tttgttttgt cagttgtgta aaagaaactc caacgatcaa
     2881 taagatcaac gcgaccattc cgcaaaaagc taagagttgc tgatacaaat aacgcattag
     2941 gcaacaccag aatcatcgaa tttgtagccg actccccaaa cagtttggat gacttgtggt
     3001 ccgacctttt cgattttttg tcgtaatttc ttgatatggg cgtccactgt ccgttcatca
     3061 ccaaagtact gataatccca gactaattca agcaactgct cccgagagaa tacttgacgt
     3121 ggtttttttg ctaaagtaaa caatagatcg aactctttag gcgtcagtcc ttcaattggc
     3181 cggttgtcca gatacgcttc tcgtgttttc gtattcatct tgaaatgact agtctcgata
     3241 tcaaaatgat catctgaaac tgctgcttct tctcttgctt ctgccaactc actacggcga
     3301 tgcaaagctt tcattcgagc aataagcgtc agaggactga aaggttttgt gacataatca
     3361 tctgccccca tctctaagcc gatcacctga tcgctttcag aatctctagc tgtcagcatg
     3421 ataatcggca cagtacttga aactttgcgg atctctcttc ctactagcat accgtccagt
     3481 gtgggcaaat tcaggtctag tgtaatcata tcccaatgtt gtgggttttc taaaaaggca
     3541 tcgagccctt cttttccatc atacttgaaa gttgcttccc aaccttcatt taagaaaaac
     3601 atctgcatca tttcagaaac agattgatta tcttcaatca ttaaaatatt cattatcgtt
     3661 ccctccagtt tccaggtcat atttctgtaa tttcttttct gtcataataa aaataggcat
     3721 aattggaaat actagcagaa gcatttcaat cagaaaataa ttcgtatgcc cagatgtctt
     3781 taaataatag ccgatcaatg ccatcagtaa gcccataaga aaaaaccagt ttccgcttgt
     3841 cttttgagcc agccgccaat gagcgtctgt ttttgcggca agaggcgaat gatacccata
     3901 aaaaggcaga tttccttttg agggcagaat ccgaaaaaga ctgccaatta ttaagaataa
     3961 gataccaata ctgaaaaaaa tcattctttc cctcctaaat tctcttctta tagtatactc
     4021 taaattgttg actgaaccaa tatgttcagg aattcttcac gaaaggtttg tcttttgttc
     4081 attctttgtt aagataaact tattccaata aaaaaggagt tttatttgtg tcaaattatc
     4141 gtgcaatcac tttctttgat cttgatggaa cattactaga tgcgaaatca aaaatcacac
     4201 ccgaagtggc aaaagctatg aacgcattaa aagaaaacaa tgttctcccc gtcatcgcta
     4261 caggacgtac agaagcagaa atccactcta tcatggaaga tgcgggcatt acttctgcgg
     4321 tcaccatgaa tggttctttc attcgagtag acggcgaaga aatctattct caaacatttt
     4381 cttcagaaga atgtcaaaaa atgtatgatc atgtacagtt gaacggtcat gaactttctt
     4441 actataacga tcagaaaatc tggtgtaccg gtcatagcca aacaatgatc aatgcttacg
     4501 acttcattca ttcagatgtt ccggaaattg atcctttagg ctttaaagaa aacacagtaa
     4561 atatgttgct gatcttatct caatcaggtg acgaatacta tcacgaacat tttcctgagc
     4621 taacttttta tcggaatggt ccttactcga tcgatacagt gaagaaagga atctcaaaag
     4681 gggcaggtgt caaacgtctg caaaaagaac ttcagctgga aaaagttcct acttttggat
     4741 ttggcgatgg tccgaatgat tttgccttgt tggaagcttg cgacaacaaa atcgctatgg
     4801 acaacgccta tgacggatta aaagaacttt ctacctttat cacaaagaaa aatacagaag
     4861 gcggaattgt ccacgcattg aagtattttg atctgattta agatcttttt ttgaagctac
     4921 ctcttttctt aagaaaatcg cttatttttt cttaagaaag gggcagttat tatataaata
     4981 atgggatatg attcgctttt ttattgaatt tatcccaata tatgctatac taattttgta
     5041 tgaaaaatac acgtaaccct tgttagctaa cggggacgtt acggattcga caggcacagt
     5101 cgagcttgaa ttgcgtttcg taggttacgt ctacgtaaaa acgttacagt taaatataac
     5161 tgctaaaaac gaaaacaact cttacgcttt agctgcctaa aaacagttag cgtagatcct
     5221 ctcggcatcg cccatgtgct cgagtaaggg tcctaacttt agtgggatac gtttcaactt
     5281 tccgtctgta agttgaaaaa gagaacatca gactagcgat acagaatgcc tgtcactcgg
     5341 caagctgtaa agtgaatcct taaatgagtt gactatgaac gtagattttt aagtggcgat
     5401 gtgtttggac gcgggttcga ctcccgccgt ctccatatat gtatggttga gacagataaa
     5461 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
     5521 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
     5581 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
     5641 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
     5701 catgttcctt caaataagtt ttttta
//
LOCUS       DBNIBO010000145         5724 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.151, whole genome shotgun sequence.
ACCESSION   DBNIBO010000145 DBNIBO010000000
VERSION     DBNIBO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.151"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(98..1228)
                     /locus_tag="AC1Y77_002350"
     CDS             complement(98..1228)
                     /locus_tag="AC1Y77_002350"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIQ1575895.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="AC1Y77_002351"
     CDS             complement(1877..3079)
                     /locus_tag="AC1Y77_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1575896.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="AC1Y77_002352"
     CDS             complement(3139..3342)
                     /locus_tag="AC1Y77_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HIQ1575897.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="AC1Y77_002353"
     CDS             complement(3484..4551)
                     /locus_tag="AC1Y77_002353"
                     /inference="COORDINATES: protein motif:HMM:NF014537.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HIQ1575898.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="AC1Y77_002354"
     CDS             complement(4609..4716)
                     /locus_tag="AC1Y77_002354"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575899.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="AC1Y77_002355"
     CDS             4879..5691
                     /locus_tag="AC1Y77_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575900.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1705 a         1171 c          908 g         1940 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaa
//
LOCUS       DBNIBO010000146         5681 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.152, whole genome shotgun sequence.
ACCESSION   DBNIBO010000146 DBNIBO010000000
VERSION     DBNIBO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.152"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1287
                     /locus_tag="AC1Y77_002356"
     CDS             <1..1287
                     /locus_tag="AC1Y77_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HIQ1575901.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIY
                     APQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVC
                     LTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="AC1Y77_002357"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="AC1Y77_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HIQ1575902.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /gene="mobV"
                     /locus_tag="AC1Y77_002358"
     CDS             3322..4584
                     /gene="mobV"
                     /locus_tag="AC1Y77_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HIQ1575903.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4877..>5681
                     /locus_tag="AC1Y77_002359"
     CDS             4877..>5681
                     /locus_tag="AC1Y77_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HIQ1575904.1"
                     /translation="MDKTKSGKVRPWREKKIANVDYFELLHILEFKKAERVKDCAEIL
                     EYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVAEVIKQKPTVRWLFL
                     TLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMRATEVTINNKDNSYNQH
                     MHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKVQMIRPKNKYKSDIQSA
                     IDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYGGLLKEIHK"
BASE COUNT         1955 a          805 c         1166 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caagaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa a
//
LOCUS       DBNIBO010000147         5584 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.153, whole genome shotgun sequence.
ACCESSION   DBNIBO010000147 DBNIBO010000000
VERSION     DBNIBO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.153"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(487..1296)
                     /locus_tag="AC1Y77_002360"
     CDS             complement(487..1296)
                     /locus_tag="AC1Y77_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1575905.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1296..2042)
                     /locus_tag="AC1Y77_002361"
     CDS             complement(1296..2042)
                     /locus_tag="AC1Y77_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1575906.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2060..2527)
                     /locus_tag="AC1Y77_002362"
     CDS             complement(2060..2527)
                     /locus_tag="AC1Y77_002362"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1575907.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2527..2937)
                     /locus_tag="AC1Y77_002363"
     CDS             complement(2527..2937)
                     /locus_tag="AC1Y77_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1575908.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2948..3961)
                     /locus_tag="AC1Y77_002364"
     CDS             complement(2948..3961)
                     /locus_tag="AC1Y77_002364"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1575909.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4178..4912
                     /locus_tag="AC1Y77_002365"
     CDS             4178..4912
                     /locus_tag="AC1Y77_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1575910.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4896..>5584
                     /locus_tag="AC1Y77_002366"
     CDS             4896..>5584
                     /locus_tag="AC1Y77_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1575911.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1789 a         1184 c          920 g         1691 t
ORIGIN      
        1 agcgacaaaa accacgagca ggtccattca tcctcaaacc tcggtgatct tcgcggttta
       61 acaccatcag aagaaatgca caaaaataat aaggagagtc tgagacataa ataaattccc
      121 agacttttag tgagtctaaa tccgaataaa cggtgggtcg aaagcaactc tagctgcgcg
      181 tcgatcgcag caatctctag gtgctatgtt cggataaatg gtggtcaaaa gtcagatttt
      241 cggccatttc gtaacagcca accagacaaa aagcgtctgt tttgtcttct actccaattg
      301 ctcaaatctt agcgactttt ggcttggtct tactctaaac agtttgtaag cctaaacttt
      361 atttgctgtt tgtttctaag atcaaagcaa aactactttt tagttggtta cttttacaag
      421 ttctttttac tgttgaataa atgtaaatct taagaacaaa tatttacttc tataattcca
      481 agtttcttaa aaaattccgc caagtgtacc taaaaagcat aaaacaaaaa ttcctagaat
      541 aattgtgatc gttttatgcc ctttggagta aagccagacg cataaaaagg taattcctaa
      601 tggcagcaag cccggaaaaa tttgatcaaa aatactttgt aatgaagttt cagctccacc
      661 aaccgtgaat tttagtgacg tatttaaagt aaccatcgtg cctatcatcg cgccaattac
      721 cgttaaacct aaaatcgaag caccatgaga taatttgtca atcatatggt tgtcttctgt
      781 atcatcaata aatttagttc ctaaaacata gccatatttt gtcgccaacc atttaaaaat
      841 aaaatgaatg gcagtataca aagtaaaata aaggatcatg cctaaaatat tcccttgttg
      901 agcaaaagaa atacctatgc cagccgcaat aacccgaaaa gttccccaga aaaatgaatc
      961 gccaatacct gataaaggtc ccattaatcc tgttttgatt ttattaattg cgtctgtatc
     1021 aaattcagga tttttagcat tttcttcttc catggtgacg gacaagctca taacaaaagg
     1081 cgcaatatgc ggtgtaatgt taaatgtttc cgtatggcgc tgcaacgctg tataataatc
     1141 atcatcgttc ggatatattt tacgtaagga acgttcaata ccattcattt gtccatatgc
     1201 catcatttta tcgaaggacc aagaggattg cataaaaaag gagcgccaaa atatacttct
     1261 taattctttt ttcccaataa cactttcttt taccattata aatcttcctc ctcttcaaaa
     1321 gttagctttt gaacagccag taacgctaag caaagtccga cgacagccac accgacaaca
     1381 tccattttca gatacattac gccgacaaat cctaagaaaa gataaggcat catcgcttta
     1441 ttcgccatca ttttaattaa taacgctagc ccaacacaag aaagcacacc ggccgcaaca
     1501 tcaaagctgt ccaatacaac ctgaggaatc aacttgatca tcgcattgat tgtagacaca
     1561 ccaaaataag tagctacaaa aactaaaatg gccgatggaa ccccaacgat taaaggttga
     1621 gccacataat gatatttttg tgcttttttg aaatcatgat tgactaatgc ctcatccatt
     1681 ttgttgttaa aatacgtaat tggcattgca taggcgaagt tacgccacat ctgaagaaag
     1741 atggacaaag gtaaagtcaa cgcaacggca gttcctactc cttgacccga ttgaatcgca
     1801 attgctgtac ctaaaacact ggaagcataa acttcagggg gcgtcgcact ccctaccata
     1861 attgagccca tgaacatgga ttccaaggtg gcaccaataa taattcctga ctctaaatct
     1921 cccaaaatca aaccgacaaa aggcgctgta aatagcggtc tacccatcat cgatgcacca
     1981 aatgtgtatt catcaataga cgccaaaaaa gctaccagtg cgattaatat tgcttgtatc
     2041 atattcttcc cctcctcttt tagtcatgca aaggattgat ttttttgtcc gttggtacag
     2101 cctgacattg aactgttatc ccgttttcag tcattttttt caccaagtcc agttcttgtt
     2161 cattcaagta aacggaacta gtaatcattt tccgattctc gtttttcttt aaatttccta
     2221 aattcacttc tttaatctca ggtacgtgct gagtaatttc gtaggcatcc tccaccgtcc
     2281 cgacaattaa aaatacttgg tatttcttga ttttttctcc atttaaaaca gagatggcct
     2341 ctgtcatatc cttcaccaca aattttgtcc ctgtaggaac agccattttt agtgtttttt
     2401 tgcgcagatt attggtagcg atttcattac ttgcaacgaa aatacaattt gcacctaagt
     2461 tatttgtcca ggcaaaagca acttgcccat gaacaaaacg ttcatctact cgtaataatt
     2521 taatcattaa aaatcatcct cttctgtttt ctctagcaac tcgttcacca aaactatccc
     2581 ttcattcccg ctattcatag ctttttggat tagttttttc gtgtcctgtt cacctgataa
     2641 gcaaagctct aaaagcagcg gcagatttac accgctgatt aaataaaaat tgtctttacc
     2701 gatttgttcg gagaaagcgt tagacacgct tcctcccagt aaatctaccg tcacaattaa
     2761 ttcgtcatca ggcgctaagt cggaaaatag ctgctctatt tcctggttaa tggagtagtc
     2821 actatctata tacgcagtta ttgctaaaag tgatttttga ggaccaataa taaattctaa
     2881 agtatccttt aagcctttag ccatcatgga atgcgtcgca attacaattt tcctcatagc
     2941 tcttacctta atattccaca gtccgataat aacgacgaat atctagtgaa tgatcccgca
     3001 ccttttcaaa atgagcactc actcgttcta aggccgttgc cataaccatt ggcgctacat
     3061 actgacgcat agcgcttggt acacctgcta attcgaaatc cgcagtgtca aaaaccgtca
     3121 caacatcgct atatttattt acaaaacgct ctacgcgatc gactagcgga cgcgtttgat
     3181 cttcaccttt taataacaca aaactcatag atttatcagt catttcaata gtcccatgga
     3241 aaaattcggc tgcatggatc gacttggtgg caatccattg catttcttcc agtacgcaca
     3301 tagcatattc ataggcagcc ccataagtat tcccggctcc aacaaacata tgaaattccg
     3361 tatttttatg tttttctgca aattcaagtg ctttctgatc attttgttgc cgaacactta
     3421 gtaatacttc cggcattttt tgtaatgttt gaataaattc atcatattca ggaaactcac
     3481 cgttattttt catcaggcgt gcacccaaag caaaaaactg taactgaagt tcttctacta
     3541 atgtatcgtt gtcagcatca ttttcaatcg cataatccac gatttccccg agtggtgtag
     3601 cagcatttcc tacaaacgca atcgtcgttg caccttgact attggcatac ttaacaacat
     3661 caacggtttc ttgagtcgta ccggttaatg aagatgtgat taacactgta tctttattaa
     3721 tctttttcgg ctgtgaacaa accacctctg cggctatttc atgataataa tcaagctctg
     3781 aatgagtgct taacagataa gctagcgggc tgattgtggc atatgttcca ccagagccta
     3841 ccaaataaag atttttaaat cccttctcac tgattgcatc tgccacctga ttgattgtct
     3901 cgatattttt tactgcacca tttaatactg atagaaaacg ttcttcgcta aaacctaaca
     3961 tagttaaaaa cctccaagtc aataatttga aaaatatatt tgcaaatgcc ggcacattaa
     4021 caaaccactt aacctttttt atataccacc tgttaacgtt tttaatgtac cacttataga
     4081 aaacgctgtc aatagttttt aaccactttt atttttattt gtaccaaaat ccttttttca
     4141 tgtataataa acaaaaatag actctggagt gtaacgtatg aatactgaag aactaacgaa
     4201 caaattaata caagacattg aacaagaaaa atataaatac gcgcaagcta ttccttcaga
     4261 acgtgatttt gttcaaattt atgaaatgaa tcgttcagcc gtaaaaaaag ccattcaaca
     4321 attagtaaat aaagagtacc tactaaaaat acatggcaaa ggaacttttg ttaaaaaaaa
     4381 tatccatgaa catttgcaaa tcaaatttca tggtttgtct gaattgttgg aacgggaagg
     4441 gattcagcct agcagcttaa ttttagcttc agaaataaaa ccggctggac acattgtcgg
     4501 gaaagcttta cagctggaac caactgaaga agtatttcat ttggttcgtt taagaagcgg
     4561 cgacggtgag ccaatttcta ttgaagacac ttatattcca ttaaatctca tacctgatct
     4621 tcaagctttt gatttccaaa ttttttcatt ttattccaca ttggctgctt atcattatca
     4681 aattgaaaat atctatcaga caatttccag ctcaaaagtg agcaataaag aagcccgttt
     4741 gttaaaaacc agcttaaatc gtccggtaat cagccttgct atcactgcgg ttacgacaaa
     4801 tgaacaagcg gttgaataca ctgaagtagt ggttttgcct gaattcgccg ccatctatac
     4861 taaaaatgtt catcaaggaa aggagatgaa aatctatgcc caaattgatt aatctgttgg
     4921 cttatgacct gattgacaaa attattgctt atattcatga acatcattta acccccggaa
     4981 caaaactgcc ttcggaacgg gacctatcag aaatgtttaa tgttaatcga actaccttaa
     5041 gaggcgcgtt acaaaaactg gtggacaacg gcaccttacg ggcgcaaacc aatagcggct
     5101 attatttaac tgcgccaaaa ttcgaaaaaa atgcccatca tttctttaca ccgatagaag
     5161 attctctgct tagtaagcag cggctacagc taaaggcaac ggctctttca ccgaaaaaag
     5221 cagcagctct ttgtccggaa ctgaatgatt tcaaacagca gcaaccacaa gcttatttag
     5281 aatatattga tgcagaagca attagcttga tgttattaat ccctatggaa acattaaaga
     5341 aaagtaattt ctcggacaat cctttccaag attttaaaag tttggcatat acgcgttctc
     5401 aagaaatttc tttaataact gacaatgacg ttgcggcgga cttgctggga gttgacaaag
     5461 aagaggttct gttatttgta cgagaggaaa taagagccgg taaggagttg gcggcgattg
     5521 ctttaggtta tactattagt cgccgctgca acatacaggt taatgttgct acgaccttgc
     5581 aatc
//
LOCUS       DBNIBO010000148         5550 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.154, whole genome shotgun sequence.
ACCESSION   DBNIBO010000148 DBNIBO010000000
VERSION     DBNIBO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.154"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="AC1Y77_002367"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="AC1Y77_002367"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIQ1575912.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="AC1Y77_002368"
     CDS             complement(1023..2126)
                     /locus_tag="AC1Y77_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIQ1575913.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3151)
                     /gene="pplA"
                     /locus_tag="AC1Y77_002369"
     CDS             complement(2216..3151)
                     /gene="pplA"
                     /locus_tag="AC1Y77_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HIQ1575914.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="AC1Y77_002370"
     CDS             3453..5387
                     /locus_tag="AC1Y77_002370"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIQ1575915.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         1609 a         1090 c         1107 g         1744 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgttata
//
LOCUS       DBNIBO010000149         5509 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.155, whole genome shotgun sequence.
ACCESSION   DBNIBO010000149 DBNIBO010000000
VERSION     DBNIBO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.155"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(67..537)
                     /locus_tag="AC1Y77_002371"
     CDS             complement(67..537)
                     /locus_tag="AC1Y77_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HIQ1575916.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            867..2504
                     /locus_tag="AC1Y77_002372"
     CDS             867..2504
                     /locus_tag="AC1Y77_002372"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HIQ1575917.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQF
                     VQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLNI
                     SGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDTE
                     GLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAAH
                     TPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNAL
                     MYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIAE
                     TVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2658..3980
                     /locus_tag="AC1Y77_002373"
     CDS             2658..3980
                     /locus_tag="AC1Y77_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HIQ1575918.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4048..4482)
                     /locus_tag="AC1Y77_002374"
     CDS             complement(4048..4482)
                     /locus_tag="AC1Y77_002374"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575919.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4547..5383)
                     /locus_tag="AC1Y77_002375"
     CDS             complement(4547..5383)
                     /locus_tag="AC1Y77_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIQ1575920.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1645 a         1098 c         1003 g         1763 t
ORIGIN      
        1 tttttcacga cctctgattc acggtgtttc tttttcatca acatttttac gaaatactgt
       61 ggaagactat tgaaataagt gtggattgtt tctttgtgtt tcaatcgttt cttgccattc
      121 ttcatttaat tttggattac cgttttcttc cttgaactca taaggtccta attcaaaaat
      181 cccagacagt atttgattca cgatttcata tagttcagtg tgatcagaca catgattctt
      241 gaagatcatc tcattatcac cacgtttgaa cctcacattc gggctctcat gatgaacctc
      301 tatgcatttt tctccttttt ttaactgacg gacttctgaa aacatctcat aaatatcttc
      361 tccagtaaaa ccttcagcaa gcgccagact gtccacaaga ccgcctactg tcagttcaga
      421 gattattgct tgattggaat ctaattttaa tgtatagctt gtcgcttcta ctacgccaca
      481 tgcattgcaa ccgccttctg atagttcgct ttttctaatc aattgaactt ctttcatttt
      541 ctcttcctcc tcttaatttt ctacttatgt atttttatca cctgacatag gtgactccta
      601 tcaacgctat catgtgcttt tttcctcaga aatcctattt atccattcca tctatccctt
      661 attcttctac ttttctttct ctagcatata agagatacgc atagtattca agcaaggatt
      721 gtcgatataa aaagaacaaa tactgctatt ttgatttctt ttggctatat tacatataaa
      781 taaaatttat catacctgct ctttttgact atatcatcat ctgacttcta tacttcgttt
      841 gtattaaaac ttaggaggaa tcattcatgc ttacagggtt aaacttatta aataatcctt
      901 ttctaaataa aggaacagct tttacaaaag aagaacgggc aaaatatgga atcactggta
      961 tgcttccaag tactgtccaa acactggaac aacaatctgt tcaagcttat ggtcaatact
     1021 taagcaaaca aacagatttg gaaaaacgta tcttcttaat gaatctattc aatacgaatc
     1081 ggacattgtt ttataaattg atgggcgaac atttagttga attcatgcct gtcgtttatg
     1141 atcctgtcgt tgcggattca atcgaacaat acaacgaaat tttcttgaaa ccgcaagatg
     1201 ctgcatttct ttcaatcgac gaaccagaaa acatcaaagc aagtttgaag aacgcggcag
     1261 atggacgaga cattcgtttg attgttgtga ctgatgcaga aggaatctta ggtatgggcg
     1321 actggggtgt gaacggtgtt gacatcgcta tcggcaaatt gatggtctat actgctgctg
     1381 gtattaatcc agcacaagtt ttgcctgtat cgatcgacgc tggtactaat aatgaagaat
     1441 tattgaataa tcccttatac ttaggcaatc gtcatgctcg tgtagaaggc gaaacttact
     1501 atgaattcat cgatcaattc gttcaatcag ctaccgaatt attcccagaa ttgttattgc
     1561 attgggaaga ttttggacga ggaaatgctg cagcgatctt agaaaaatac gaagatcaaa
     1621 tcactacttt caacgacgat atccaaggta caggaattgt tgtcctagca ggtgtattag
     1681 gcggattgaa tatatccggt gaacaattga aagatcaaat aattttgaca tttggtgctg
     1741 gtactgctgg tgtgggtatc gcaaacatcc ttttagatga gatgatccgt caaggtgtac
     1801 ctgaagaaga agcacgccaa catttctatc aagtagataa acaaggttta ttgtttgaag
     1861 atacagaagg attgactcct ggtcaaatcc cattcgctcg caaacgctca gatttcacta
     1921 atgctgatga attgacaaac ttagaagcag ttgtaaaagc gatccatccg acaatcatga
     1981 ttggtacttc tacccaacct ggagcattca ctgaagaaat cgtgaaagaa atggctgctc
     2041 atacaccacg tccgatcatc atgcctttgt ctaatccaac aaaattggca gaagcaaaag
     2101 caaaagattt aatagaatgg acggacggaa aagcattagt tggaacaggt atccctgctg
     2161 ctgatgttga atataatggt gtaacttacc aaatcggtca agcaaataat gctttgatgt
     2221 acccaggttt aggacttggt ttgattgctt caacagctac acgagtaaat gctgaaatca
     2281 tttctcaagc tagtcgagca ttaggcggaa tcgttgatgt aacaaaacca ggagcagcta
     2341 tcttgcctcc agtagcaaaa atcactgaat tttctcaaac gattgctgaa actgtcgcaa
     2401 aatcagttgt cgcacaaaac ttgaatcgtg aagaaatcac ggatatcaaa gaagcagttg
     2461 aatctgctaa atgggttcct gaatataaat ctttagaaga ttaagatcgt tatagtaaaa
     2521 gaacatacag aaactttgta tgacgtctaa aaagaaaaaa gcgactgcgg caaaacagcg
     2581 gccagccaac tcctgctctt ttgtcgcttt tctttggctt ttttcatatg atacagaata
     2641 agaaaggatc tgtaaaaatg gcacaaaaaa caacaactgc aactgcagtt gaggaaaaga
     2701 aaaaagaagg tttttggtcc acaaaaatca ccggcgtaag tttgccactt tatttgatca
     2761 tggtccttgt cctgatcatt gcaatggctt tagggaaatt accgacgaat atgattggac
     2821 caatcgcagt tttggttatt ttcggtaatt tatttcattt tatcgggaca aaaatcccta
     2881 tcgtcaaaag ttatttgggt ggtggttccg tttttgcgat cttcgcttct gctgcgattg
     2941 ctacatttgg gatcttacct gaatatgtag tgaaatccac agaaaacttt gtcagcaata
     3001 tgggcttttg ggacttttat attgctgcct tgattactgg tagcatcctt ggtatgaacc
     3061 gtaatctatt gatgaaagct tctgttcgtt ttattcccgt tgcacttatt tcaatggtcg
     3121 tctccttttt tgccgtagga ctcgtgggca tgcttatcgg aaatggtttt gcaaattccg
     3181 ttttatacat ctcattacct gcgatggctg gtggtgttgg agccggagcc gttcctttat
     3241 caacgattta tgcaaatgtt ttaggtacgg atgcttcttc tatcatttcc cgcctgattc
     3301 ctgcgacagc aatgacaaat gtcttagcta tcatcggtgc agcattagtt gctcgtgctg
     3361 gacaatcaat gccaaaattc aacggcaatg gtaaattaat ggaaaaaggc gatcttggcg
     3421 atggaaaacc tcgaactgtg aaaccttcca ttccacagtt aggtgttggt ttgatggtat
     3481 cattgacttt cttcatctta ggacaaattg gtaactattt tgttcctaaa gtccatgctt
     3541 acgcatttat gattattatc gttgtcatct gtaaaatcgc caacctatta ccagagtatt
     3601 atgaagatgc agctatcatg ttcaataact tgattgtcaa aaacttgaca gcagctgttc
     3661 ttgccggtat cggtattgcg ttgctgaatc tgaatgtatt agcttctgct ttgacatggc
     3721 aatttgttgt cttatgtctg acaagtgtta tcgcgatttc agttgtttca ggatttgtcg
     3781 gtcgtttgtt tggtttatat ccaattgaat caactattac tgctggctta tgcaacaact
     3841 cgatgggcgg aacaggaaat gtagccgtct tatctgcagc taatcggatg gaacttattg
     3901 cttttgctca aatgggcaat cgacttggtg gtgccatcgt tttgatcatc tctggtttct
     3961 tgatgcaatt actttcttag tcaaaaaaac aaaaatacct gatcatcaag tatcggcttg
     4021 gtgatcgggt atttttttga taaagattta caggattttc aaaaatcttt tttgcatttt
     4081 atcggaatga taaccgatgt gttcataaaa ttggtgtgct tctactctgt attctgcaga
     4141 attaagcctt attgtcgtta ttcctcgttt tttcgcttct tcttctacgc cagtcatcaa
     4201 agcctttccg atgcctcttt tttcagcata tttagacaca gcaagtgcca taatattgaa
     4261 cattggatcg ctaaaaatcg attcatagga ctccgcttgt acatagccag ctgctttttt
     4321 agaagtagga tcttcataaa ccaagaaaaa atttttctct ggctccttca gcagtttctt
     4381 catttgtctc accgtaacgg ttaatggtat atcataccct aattgatccg cattgatttc
     4441 acgtagtgct tctgcatctt ctaaacgcat ttcccgtaac atccaaatca ccagccaatt
     4501 tctgattttt taaaatgaat ttttttatca tagtatactc tttttttcat ttagaacaat
     4561 atgtttcagt gattttgact tgcgttagtc tagattctcc taatgtttgt aagatgacaa
     4621 aggcattgta acaattgacc aaaagaacca cgaggacaat tacttgaaaa ataaaggtca
     4681 ctttttttgg agacaaaacc ctgctaaatc ttgctcccca caaacctcca ataacagcag
     4741 cggggataac ataaaacaac atagataaat caaagcgcat aaagctactt gttaaagcaa
     4801 tcgttaccaa ttttgccagt tgagaaaaga aaatcgtaga tagagaatat agtgttgctt
     4861 cttttattgg caatgcaaac atcagcatca acaaagaaac attgactggt ccaccaccaa
     4921 tacctaaaaa actagctaaa aagcccagaa cccccccaca tataaggtac caagtccatg
     4981 acgtcaaatg aaattttggc tgatgatgct tggtgtagaa gaacgcaaaa accaaggtca
     5041 ataccgtcaa aaaaatttgg atcagctgca catgcttctc gttttcaaat aactgtaaaa
     5101 aaacttcgaa tacaatgttg ccgactactc ctccaacaac agctccacta gatacccaac
     5161 cgacaatctg ccaattcagt gatttcccgc ttgatacttg tcgcacagtc gaaacaatgg
     5221 acatcgtgaa taccgcaact gttgagtaaa aagaaatccc tgcaacagaa tgggcgccga
     5281 taagatcaag aatcggtttg atcaacacgc ctccacccat cccggaaata gcgccaactg
     5341 tgttcgccag aacgatcacc acaaaataaa tcagaccaat catcttgtat ttcctttctc
     5401 ttgtttatat cttttagcga ttgttactct tttcttttat tatacagtaa aagaaacaaa
     5461 cgaggcttcg aaagcttttc tactttttaa ggaaatttag tgctaaacg
//
LOCUS       DBNIBO010000150         5466 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.156, whole genome shotgun sequence.
ACCESSION   DBNIBO010000150 DBNIBO010000000
VERSION     DBNIBO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.156"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            133..1107
                     /locus_tag="AC1Y77_002376"
     CDS             133..1107
                     /locus_tag="AC1Y77_002376"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1575921.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="AC1Y77_002377"
     CDS             1089..1550
                     /locus_tag="AC1Y77_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1575922.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="AC1Y77_002378"
     CDS             1600..2064
                     /locus_tag="AC1Y77_002378"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1575923.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="AC1Y77_002379"
     CDS             2078..2827
                     /locus_tag="AC1Y77_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIQ1575924.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="AC1Y77_002380"
     CDS             2827..3657
                     /locus_tag="AC1Y77_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1575925.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="AC1Y77_002381"
     CDS             3657..4304
                     /locus_tag="AC1Y77_002381"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575926.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAISSSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="AC1Y77_002382"
     CDS             complement(4402..5178)
                     /locus_tag="AC1Y77_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1575927.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5272..5403
                     /locus_tag="AC1Y77_002383"
                     /pseudo
     CDS             5272..5403
                     /locus_tag="AC1Y77_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
BASE COUNT         1872 a          739 c          972 g         1883 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat ttcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DBNIBO010000151         5336 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.157, whole genome shotgun sequence.
ACCESSION   DBNIBO010000151 DBNIBO010000000
VERSION     DBNIBO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.157"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(59..283)
                     /locus_tag="AC1Y77_002384"
                     /pseudo
     CDS             complement(59..283)
                     /locus_tag="AC1Y77_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(837..923)
                     /locus_tag="AC1Y77_002385"
                     /pseudo
     CDS             complement(837..923)
                     /locus_tag="AC1Y77_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2089..3129
                     /locus_tag="AC1Y77_002386"
     CDS             2089..3129
                     /locus_tag="AC1Y77_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIQ1575928.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3953..4285
                     /gene="traE"
                     /locus_tag="AC1Y77_002387"
     CDS             3953..4285
                     /gene="traE"
                     /locus_tag="AC1Y77_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HIQ1575929.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4904..5245
                     /locus_tag="AC1Y77_002388"
     CDS             4904..5245
                     /locus_tag="AC1Y77_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HIQ1575930.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1941 a          728 c          899 g         1768 t
ORIGIN      
        1 gtatttttat tatatataga tttaacatat gtcggtcggt tgattgatat agctaaagtt
       61 ataactcaac cgaagccgat tcatctacac gaataaagtt gcgagtgttc tcggctaatt
      121 cataaaaatt gttatgtgtt ccatatagca atactggaat aactttattt tcttgaatct
      181 cataagcaac tctatacacg gtgaccgcat acttaaatcc attagaccaa ttacctgata
      241 aacctccagt tttttgctct tacttaaatg gatcaatggc aatttcatca caaacctata
      301 gaggatgagt gacttctgcc taattacgtt aaaaaatatt gaaagtaact ctatgtagca
      361 aaaaatatct agaatttcat ttcctcaaaa ctgatcgata ttgtatctaa aaatatatac
      421 tacctaaaat aggtagtata tattaaaata tgtgcttttg ttagaaaaat caatacataa
      481 attatcatat ctgtatatgt catttaggta tttcagttat gttataatag ttttgctgtc
      541 tgctaccccg gcctaatctg gcaaggggag gaggagatat aatgaaatac ctttttgatt
      601 ttgtttttgc aatcaagcca cttgcagatg tagtgtgcag ttacctctgc aaatggcttg
      661 attgcaaaat tgccaaaaaa gacagtaagc ctacccgtta attctataat atagaaacgt
      721 aaaaaaaacc ccatcagcct gcacgctgat ggggtttggt gatataatga aatacctctt
      781 tgttactcat aattatacca taattagtca cgaatgcaac caatagaaaa aactacttag
      841 aacggtagat catcgctaat aacaatgcat tgtccaccaa atggattttg tgaagcatta
      901 aatacattgt tattaattgt cgctgctttt aactaggcct ctccccttct attataaaag
      961 aagatatata tcatttctta attacctact accttaaacc aaaatttaag tactacctaa
     1021 tttaagtagt acttaaacaa tctaaaaaat agttatccca attgttatgc ttttataata
     1081 cgattatttg taacaaaatg aatattatat aattaaacac taccatattt aggtagtgtt
     1141 taattattag caaaacaaaa aatatttatt ctattctctt aacttcaact aagttatgct
     1201 gcacaccttt atataaatca tatgcaactc attagctaga caaactacaa gcatgtagtt
     1261 ttatctgtta attatgtact acctaaataa agtagtttat aattagcaag tatatatatt
     1321 taaaaattta agattaattt ttttatgact tgtacaaatt tatctgatat tctcttatta
     1381 agaactacct aatttgggta gttcttattt tttacatttt aagtagtatt taattgcaat
     1441 ttggtagtat ttttttatta tcttattctt agaacggtag tacttaatat aaatataggt
     1501 agtatctaat ttaagtatta atggtagtat ctaaattaac aggtagtatg taactacttt
     1561 aaagtaattt ttttcagatt aacacagtag tatcttatta aataagttaa ttttaatggt
     1621 agtacataat tattatatat atctgacatt ggtagtactt attgtatttt ttagtagtat
     1681 ataatctatt gttttctagc ctaaagcctt gtcttataag aataatataa tatatactac
     1741 tgaaataagg tagtatatat tactagtaat ggtagtatgt tttatatgca taaggtagtt
     1801 cttaatttat tttttgtttt gacactggta gtacttaatt atctttaatt ttaaaactaa
     1861 attttattca tacgtttttc tggtagtatt taattataaa taatatttat ttaaagtagt
     1921 tcttaatgac tttaaaggta gtacataatt cattttattt ctgattttag gtagtattta
     1981 attaaaaaac agttttaatg cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga
     2041 gtaggtaatt ttacataaat aaaacaacgt gaaagtagag gatctacaat gactgatttt
     2101 aaatttttta aggcggatag agtttacaac gaattatttt atcaatttcc aaaagtcttt
     2161 attgtttctg acgaatacaa aaaaatgaaa gattcaacta agattgccta tatgctttta
     2221 aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg taatgtttat
     2281 tttacttata cgacaaatga actatgtaga gtattaaact gccaaaaaca aaaagcgata
     2341 gcaatcaaaa aagagttgga atcctttggt ttattattac aaaagcagat gggatttaac
     2401 aaacagttag ggaaaaataa tcctaataga ctatatctag cagaattaaa agtctcagaa
     2461 aatgatatct acttactcga aaaatttgat agagagaata gggaaaacgt tgataaatca
     2521 gagggtatga aaatcatacc caccctcgac gaaaaatcag acgctgaatc ccttggggct
     2581 caagagggta tgaaaatcat accgtgccaa aacgttgata aatcagaggg tatgaaaatc
     2641 ataccagaac ttaataataa tatattagac actaatagac acaatataga cactgaaaaa
     2701 gaccgcctac aagatcaatt gttgttagac aattttgaga caattatgac aaacgacagc
     2761 attgctacgt ttgtccctga acgagtatta aatttgataa aaacattttc ttcaagttac
     2821 agtgaagctc aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc tgaaatagaa
     2881 agtgatattt cgatagtttt tgaagaactc gatagttatt atgtcaatgc agaacaagaa
     2941 ttatacacga cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc
     3001 cagaacctga tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa
     3061 gtatcaagtg aaaaacgttt gaattatgca agctccccaa gcactattac gaaagactgg
     3121 ttagagtgaa actttactga caaagtgatt gttgcttcat ggagtaacat taaaattaaa
     3181 aattttttat tattctaatt atattacgaa tattacaaaa taatagctat tttaatagaa
     3241 tttataagtt aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt
     3301 aaaagaaata attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt
     3361 ttgagttata ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt
     3421 gatattactt cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta
     3481 attgtctgct atgcttgtga tgaattgatt ttcgttttgc acttttggat gataaatttg
     3541 gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg
     3601 tcagcgttca tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg
     3661 gtattacccg caatggattt agtataacag atattcaaaa gtgataccac tttttttggc
     3721 tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca taatgttata
     3781 aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg aacgcatgtt
     3841 tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta tttttgaaaa
     3901 ataacaagaa aaacttgtta aaaatattta aagaaattgg gtggtttatt ttatgtcata
     3961 tacaggaatt ttgagtctca aagatatttg tcactacggt aaacggtgta ccgctacaga
     4021 gaaaatcacg aagaaactat caactggcca aagtaaaacc gttgtgcagt gtaagaaata
     4081 tataatccag aaagacaaag tctcagagga aatgatttat tacattggga aacaaaagca
     4141 aataatttta aaagatccta tccccttaaa agagctatat ccaacaatca aacatgttta
     4201 tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa
     4261 agggatgggg cgtttgatcg gctgaattga tcattctcat aacttgtatt ttatcgtttt
     4321 tcgcttgctt gaaaatattg tcgagacttc taaaggaact tagagtcact agaaaaataa
     4381 ggagtagact tgatctatca acaattccct tcggggagtg acggggtagg ttttaacgat
     4441 tggtaacgta aaaaattggt caatcgagta aatgcgcttg tttggatagt ggtgaaatga
     4501 atttgtgtaa ataataagcg cagagtaaac aagagggaat ttagaagtta gacaagttag
     4561 aatagcgtct agattaatca tgagcagcta ctaagggatc tagactttga cgcaaactca
     4621 tggaataagg agtaggtctt ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc
     4681 tgcagtacat gtagatactt gcgaggacgt tgattattgg atcaatcgag catttaccgc
     4741 tgtctactag ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt tatgtatttt
     4801 taagtaaacg aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa aaattaatat
     4861 aaagcataga gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa attatgatcc
     4921 gaatatacga ttgggcacac atactattaa ggtttctttc caacgttggg attacaaagg
     4981 atttgtgacg tttagaaggg gcggaaattg taaaggatta gatgttttag cattagacga
     5041 ggatgattta tacgatcaaa cattgactga taatccaatt ggattcggct tgctacctga
     5101 agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt
     5161 atctgttgaa gatacttggt catatttgag tgattatatt gtcagctttg aaatcattga
     5221 atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta
     5281 gaaggagtca cgacgattaa taaaaaggta gtattacctt aggcattgag taagga
//
LOCUS       DBNIBO010000152         5275 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.158, whole genome shotgun sequence.
ACCESSION   DBNIBO010000152 DBNIBO010000000
VERSION     DBNIBO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.158"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..263
                     /locus_tag="AC1Y77_002389"
     CDS             <1..263
                     /locus_tag="AC1Y77_002389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575931.1"
                     /translation="KKKNPAQTSCLFFSNRFAHEINCFSHHRQFIPFTSFNLTATRHK
                     GVIATLSHFAYSSRLRSFVKVKTVVQGAVGIGVKACCSKFLD"
     gene            439..537
                     /locus_tag="AC1Y77_002390"
     CDS             439..537
                     /locus_tag="AC1Y77_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HIQ1575932.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
     gene            complement(691..1188)
                     /locus_tag="AC1Y77_002391"
     CDS             complement(691..1188)
                     /locus_tag="AC1Y77_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575933.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRV
                     VFALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQ
                     ALKIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYF
                     ELNSQ"
     gene            complement(1216..1704)
                     /locus_tag="AC1Y77_002392"
     CDS             complement(1216..1704)
                     /locus_tag="AC1Y77_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575934.1"
                     /translation="MIVVAWKGSDDMPLDDFASKQELQKVKQELAEIKQQNQEVIETL
                     KSLDNSVKQGYIKVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSV
                     SDELSRQVRPQVQKFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLK
                     LL"
     gene            complement(1679..2665)
                     /locus_tag="AC1Y77_002393"
     CDS             complement(1679..2665)
                     /locus_tag="AC1Y77_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIQ1575935.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLA
                     QTQLEATRRIFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPN
                     HEIYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQ
                     PKEKFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNK
                     GKRIGFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVT
                     NDDFSISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
     gene            complement(2650..3006)
                     /locus_tag="AC1Y77_002394"
     CDS             complement(2650..3006)
                     /locus_tag="AC1Y77_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIQ1575936.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLC
                     KSVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQ
                     QLSQIEKGVIDLWEQL"
     gene            complement(3562..3927)
                     /locus_tag="AC1Y77_002395"
     CDS             complement(3562..3927)
                     /locus_tag="AC1Y77_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575937.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFM
                     RNASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLD
                     YKTFYPELLEFFQKLNSLE"
     gene            complement(3936..4097)
                     /locus_tag="AC1Y77_002396"
     CDS             complement(3936..4097)
                     /locus_tag="AC1Y77_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575938.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLT
                     EIIELDSKE"
     gene            complement(4087..4236)
                     /locus_tag="AC1Y77_002397"
     CDS             complement(4087..4236)
                     /locus_tag="AC1Y77_002397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575939.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGE
                     QKNDG"
     gene            complement(4250..4999)
                     /locus_tag="AC1Y77_002398"
     CDS             complement(4250..4999)
                     /locus_tag="AC1Y77_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HIQ1575940.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVL
                     DYCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVE
                     RPDGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGK
                     YALIMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELS
                     DKMSVSFFVTTLKRGRKVVGYEITINDTSKE"
BASE COUNT         1374 a         1195 c          975 g         1731 t
ORIGIN      
        1 aaaaaaaaaa aaatcccgcc caaaccagtt gtctattttt ttccaacagg ttcgcgcatg
       61 aaataaactg cttctcacat catcgacaat ttatcccctt tacctcgttc aatcttacag
      121 ccacaaggca taaaggagtg atcgctacgc tctcacattt tgcttacagc agtcgcctgc
      181 gctccttcgt taaggtcaaa accgttgttc aaggagcggt tgggataggc gtaaaagctt
      241 gttgtagcaa gtttttagac tgatttagat taaatcgtta gtcttgttat acagcgcctt
      301 tacaggcggt cagggcaagc cctaacgacc caaaagcccc atctggtaga ccatcttttt
      361 tttgcaggga gtgaatgcta tattaatctt ggcactaaag ccaactaacg cttacacagg
      421 ttgggaggtg tattccgtat gtacgaaaaa tttctaacgt tggttgtcgc accgctattt
      481 gtcgggctag tcattgagct agtttctcat tggttggacg agaaagacga caactaaggc
      541 ttagtgcctt ccaactcaca cgcctagtcg cagggcgaaa aaaaagccag acaccgtcgc
      601 atggtgtctg gcttttgtgc attccgcact cgaaaaaatt ctaacgtttt cgcatggtta
      661 ttatagcatg gacgttggca aatctcaatt tcactgacta ttcagctcaa aataggattc
      721 tacatcgtca aatcgctttg tgtagcggtc tttcagctct ttatcgggca aagtagcgat
      781 cacgtcacga tttttttgta cccaatccgc cgtaataact tcgcttggat agtcttttgg
      841 gttaaaggtt ttcagctttt cttgactggc aattttcaat gcctgctcca gttggtccga
      901 aattttatca atttttttct gttttgcttc ggctttcgct tctagttgct ctattttgct
      961 gattttaggg cgtacagaca gcgcaacgtc cggacggtat gaagaaatgc cgaccgacac
     1021 aaaaagcgct agaaacgcaa aacagagggc aaaaacgaca cgtttcagct ttggttcctc
     1081 aaatttttct aagatcgagc gaaaaatgca gaataaacta tacgaaccga ccaaaataat
     1141 cacgacagga agaatatgcc accccatttt ttcaaagaac agtttcattt gcgttttaag
     1201 ccccctctcg ttcgattaca ggagctttag gactgaaaca ataagaatag cagtgccacc
     1261 gataaaaagc cccacagagg ctaatacgga cattaaattc gctattcgat tttgtttcag
     1321 ctcttcggca gtcgctcgat catcgttggc taaaaatttt tggacttgtg ggcgcacttg
     1381 ccggctcaat tcgtcagata cagatttgct gatattaccg tagctatcaa gcttttggtc
     1441 gatttcagcg gttctttttt gaacgtaggt ggcgtagcgg ttcatttcag ctttcagctc
     1501 gctcattttg ggaatatctt cgccacctcg caccttgata tagccttgtt ttacgctgtt
     1561 atcaaggctt ttcagcgttt caatgacttc ttgattttgt tgcttgattt ccgctagttc
     1621 ctgcttcact ttttgaagct cctgcttact tgcaaaatca tctaacggca tatcgtcact
     1681 acctttccat gccaccacga tcatgataga acgggcgaga acgttgttga cgctcgattt
     1741 ctttttgtcg ttgcagctcc tcttttttct tgcgttcttc ttgctgacga atatcattag
     1801 caagattttt caaaccgctg atagaaaagt catcattggt gactttaggc ttggtttcaa
     1861 gcggtttttc gacttctttt tcttgttttt gttgagaaaa ttcaagtgtt cgttcgatcg
     1921 cagggcgctc aaaatcagaa cctaattttc gtccgccgat tgtgaaatct ttgccctcat
     1981 catcgaccca tttgaagcca atccttttgc ctttattgta ctcataaacc tcgacagcat
     2041 aggcttcttg aagatttttt ttgaaagaat caaaatcggt actcatatct tgtgcgccca
     2101 ggatcatatg cttcattttt tctcgattac tcattttgcc ctcggcgtgc atttcaaaat
     2161 caacttgttt gaatttttct ttcggctgtt caatgccaaa actcaataaa accttatcgt
     2221 ttaggtcttg taggtggtag ccgacctcgg tatcagagtg aaatttttta ccggtttcag
     2281 tgtcgatact ggcgaaaaca atgtgattat gaatatagcc ggattctgaa tcggtatgag
     2341 tgcccatgta gatttcatga ttgggatagc tttttgcgat ttcatcaacg agtttagcac
     2401 cgatctcatg cgctttttgc acgtccgccg gattgttttt gtcaaacgaa ttatcaaacg
     2461 attgaaccca atgaaaggct tgaataccgc ctgttttggc gaaaatccgt ctagtcgctt
     2521 ctagctgagt ttgagccaat ttattctcag ctgacagttt gtgcgtataa atgcggtcac
     2581 gatcaagctt tccatgccga tttacatagc caatcagcgc agaagcccca cgtttaccgc
     2641 cttttaaagc taaagttgct cccataagtc aatcaccccc ttttcgattt gcgaaagttg
     2701 ctggttcatc aaggctaaat ctgaacttga aggcatttgc cccttattca tgttaataaa
     2761 gcgagccatc tgattgagat ttccgccata tttgcgcaac tctttggcaa tttgtatcgc
     2821 cccctcccga tcaacgttcg gcgctcgata cttcatgcca agtgccacgc tcttgcagag
     2881 ctgtggcaca ctcataccag tttgatccgc aatggcggtc aaatactggt actcgtcttc
     2941 gctcactcga aatttaattt gccgactatc ttttcgattt tcggcattca atttctgctc
     3001 gctcatctca tcactcctca aataaaaatg ctccctattc ggtcgctttt ttcttctcgt
     3061 cattcttcgt ggtcgatatt caaagctaca aaaacgctcc ctattcagtc gccttttttc
     3121 cgcaatgaaa tctccctcgg ttatgccact acaaaatgaa agttccctac tcggtcactt
     3181 ccctttttcc gctctataac cgaatgattt attaacctca tcatactccc tattcggtcg
     3241 cataacaagg ttaataaatc atataatacg caagcaccgc atttggtacc caaatacatc
     3301 gcttgtcgag gtggcgttgc cccctacgaa ctcccccaat cacgcaacaa aaagtagcct
     3361 gttcggctat ttttattacg tgatttcgcc tcagaaaaag tagtctgttc gactattttt
     3421 ctactggctc atgcggtcaa acttgcacgc aagttgacct tatttttttg tttgcccgct
     3481 gttcgcaggc aaacaaaaaa gcaagccaaa aatcggcttg ctcgttcttc aaaaattgat
     3541 cgcatggact agctcgttcc gctattccaa gctgttcaat ttttggaaga actctagcaa
     3601 ctcaggataa aatgttttgt aatccaactt gtttccccag ttgtcttcca tccatttttc
     3661 gagttgcttt tgaaattgca ccgcttcggc tttcagctcg ctaggcaatt tttcggcaga
     3721 agtggccacc ggctttgcag ctgctggcgc aggtgcagat ccggcggacg acttcaatgc
     3781 ctttgacgca ttccgcatga aagtattgta ggcggaatag ctcttgaacc cttgtttttc
     3841 aaccatattt gtaccgattt tgactgaaag atcatgccga gcttcagcta attttttttc
     3901 caaatcttcg atatccattt ttgccatgcg taaactcact ccttagaatc tagttcgata
     3961 atttccgtta gaagaagatc aaattggttg atcgtttctt ctgaaagtcc accggtgtat
     4021 tcaataatag cgtcactatg cctttctaac agcttttcaa gctgttcaac gtcaatcgta
     4081 actgtcttag ccgtcatttt tttgttctcc tttttcgtca taactatgaa tcaaaaagcc
     4141 gtcacgttcg ccttttgcat agttcattag ttctttcata gcttgatcta gcgaagaatt
     4201 tttctttgcc atctttttac gcagtctttt tttcaactaa atcactcctc tattctttgc
     4261 tggtgtcatt gatcgtgatt tcatatccaa cgacttttcg cccacgcttt aaggttgtga
     4321 cgaaaaatga aactgacatc ttatcagata attcttcaat agcacgagta attacttttt
     4381 gaaaaaattg acctgcagga aggcgtttgt ctttaccaag aaaccaagat tcccattctt
     4441 ccaaatcacc tgtaatcgtt gtgtgttttt cttttcctct acggtaggat tcccagagtt
     4501 ttaacattat gagagcatat ttcccattga tccgagataa ttcacctaaa cgaaagctat
     4561 aaaacttttc tttgagatca agaacgtatg gagctgaatc agcagaaaat ttgaatctta
     4621 ctaacccgtc agcataaaaa tcaatccaag aaaataattg cgtcattctg agaccttgag
     4681 taccatcggg gcgctcgaca cgaagataaa gcgcagtatt ttcattaagc tttttgaaag
     4741 cttcagctat tcgacggtaa gaatctccag aggtgttcaa cccaaaatgt ttgataattt
     4801 ctaatgcggt tacctcgtat tcgtcatcta gttttgaatc tttagttaca aagctaaaac
     4861 agtagtctaa aactcgttgc tcaaacgatt taagccctcc aaatgcctta gctaagtcgt
     4921 ttccttgcac tactaaataa tcttgacggc ttagtaagtc ttttaatgca atatcagctt
     4981 ttttatttgg ttgtcccaaa ctgaaaatcc tccttttgta ctttttttaa aataatacca
     5041 tttattcagt ttttttgcaa tatttgaatt ttcaacgtgg aaaatagtat ttttgacgtg
     5101 gaaaatagta tttttgacgt ggaaaatagt attttcaacg tggaaaatag tatttatagg
     5161 cgctacaaac gctggtataa agccattcct tttcccctaa agtattttaa agtatttaaa
     5221 agtaatctat aaagtattct ctttaagcgc gcgggtgaga aaaaaattta aattc
//
LOCUS       DBNIBO010000153         5239 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.159, whole genome shotgun sequence.
ACCESSION   DBNIBO010000153 DBNIBO010000000
VERSION     DBNIBO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.159"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(136..1323)
                     /locus_tag="AC1Y77_002399"
     CDS             complement(136..1323)
                     /locus_tag="AC1Y77_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HIQ1575941.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1429..2316
                     /locus_tag="AC1Y77_002400"
                     /pseudo
     CDS             1429..2316
                     /locus_tag="AC1Y77_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            2364..4493
                     /locus_tag="AC1Y77_002401"
     CDS             2364..4493
                     /locus_tag="AC1Y77_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIQ1575942.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            4468..5157
                     /locus_tag="AC1Y77_002402"
     CDS             4468..5157
                     /locus_tag="AC1Y77_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575943.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
BASE COUNT         1614 a          996 c         1106 g         1523 t
ORIGIN      
        1 catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc atgcctttct ttctgaggag
       61 agaggtcagg agcagtagcg cagcgactct tgatgtctct cctcagaaag aagagactcg
      121 cacaggttca tggtgctaaa tgttattcga attaaattct aaatattttc gtggagaagc
      181 cagccactca gtgtcttttt ccatcaaaag tgttccaatt atccgagtga tggaattgat
      241 atttggaaaa atccgaatca ctcgttctct ccggcgaatc tcttcgttca accgttccaa
      301 cacattggtt gtacgaatcc ttcggcggat attttcagga aaagacaaaa tttgaatagc
      361 atcttcaaag ccgttttcta aaatgtcgca agcagcactt aattttgaat cacaaccata
      421 tttttccaag aagtgctctt ttctttcacg tgtcaattca agttcagagg ctttaaagat
      481 actcttcaac tcgtctttta catcagaaga aactttcttt ggcagcttat caaaaatatt
      541 tctcaaaaag tgggcttggc atctttgcca gctcgcacct aaaaagttct tgcgaacagc
      601 cttaactaac cctttgtgtg catcagaaat gaccagtttt acaccgaaca aacctcgttg
      661 aacgaacgat tgaaaaacgg tatcccaatt atcttccgat tcgccgtctt gaacatcaaa
      721 accaagaatc gtacgtcgtc catcagaatc aatgccgata ccaatatata caccttttga
      781 gacaatacga tgattttccc ttactttcat atacagggca tcacacatga aaaatggata
      841 ttccgagcct tctaggctcc ggtttctcca ctcaaaaaca aatggatcca actgtttcat
      901 cagatttgaa acgaatgatt tagaaacagt cgcaccatcc gttagcagtt caactgtttt
      961 agttactttg cgagtggaga caccagaaat gaccatttca atcatggcag cgatcaaagc
     1021 tttttcgctt cgctgatatc tttcaaagat agtaggacta aacttaccgt ctctggttcg
     1081 tggaacatga agttctaacg taccaattcg agttgtaaac gaacgatcgt agtaaccatt
     1141 tctttgtgat tgccgttctt ctgaacgttc ataggcattt gcctgaatat attgatttcg
     1201 ttgttcttcc atcagctgat taaacatgat agtcataagt gtttttgcgg tatcattcgc
     1261 accgctttcg tttataattg cttgaatttc ttcgctttca aaagtaaaat gtacttgagt
     1321 cattgatatc agtcctattc gagtatgttt tctgcaaaaa acattgtact cctaactgag
     1381 atcaatggct tttttgatgc ttcactttta cacaattata tggacgtaat cggtatttca
     1441 gtaccggttg cactgttctc atttatgggc tcgattatgg ctggaatctt tgctaatatc
     1501 gtcaatttca aacaatatca aacgatttat gctggtttca atccagcggc tgaaagctat
     1561 acttataatg actatttcca aattggtatg attgggatga ttgtaagctt agttgtggta
     1621 ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct atgcaatggc agcaaacgta
     1681 ccagctgaag gtggcgacgc accaatgatc tcatggttag ccgtattatt accagtttta
     1741 ggggtcgttc ttttagatct tcaaatcatc ttaggtttta ttctagcagg tatctgggca
     1801 ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa
     1861 ttatttacag atggcgcagt cgatgttgca ccaatggttg gtttcttgat gaccttagcg
     1921 atgttcaata attcagctgc gtatgcttct ccttattttt cagctatttt tggagattgg
     1981 ataccgcaat caccattagt cttggcaatc gtatttgcta tattgacacc actaggattt
     2041 ttccgcggtc caatgaatct agtcggttca ggctcagcta ttttagctgt cgtgttagct
     2101 gttaatccaa cgatgtcgcc agcctttttg ttcccgttat ttgcaattac aacgattgct
     2161 cctcagcatt tagatattac acaatcttgg gttgcttggg gattaggtta tacaaaagta
     2221 acttctcgag aatatatgaa gaaatccatc ccaactggtt ggatcatcgg tgcgatctta
     2281 tgcctgatca cgttcttatt gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga
     2341 ttagataaat ggaggaaaag aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg
     2401 gtacacatac gaaagcagtc gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt
     2461 cagtgaaaac aacccacgat gatgtccgtg gagtagctgc aggtgtcgtt cagtcttttc
     2521 aaaattgttt gcgggaaaat aatatcagtc cagaagatgt tgtgtttgtt gcacattcta
     2581 ccacacaagc gactaatgca ctgattgaag gagacgtagc aaaagtcggc gtaatcggga
     2641 tggcaaaagg tggattagaa ggttttttag caaaacgaca aactagattg aacgatatcg
     2701 atttagggaa taagaaaaaa atagagattg tcaatgcatt cttacctgtc aaaaacttaa
     2761 atgtagaccg tgtttcagaa acgatttctt ctttagaaag agaaagagcg gaggtcttgg
     2821 tttcttctat ggcatttgga gtagataatg gggaaccaga acgagttgtt tatgaagcag
     2881 catctgtgaa agcgatccct acaacgatgg cttctgacat cacaaaatta tatggattga
     2941 caagaagaac tcggacagca gcaatcaacg catctatttt accaaaaatg ctagatacgg
     3001 caacctcgac agaagactcc gtacgagaag caggggtcaa tgtttctttg atgattatgc
     3061 gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc
     3121 tttccggtcc tgctgcatca gtcatgggct ctttgatgta cttgagagcg tctaatggtg
     3181 tttattttga agtaggcgga acaacaacca atatcggggt cattaaaaat ggacgtccag
     3241 caatcgatta ttcaattgta ggtggccacc caacatatat ctcttcatta gatgttcggg
     3301 ttctaggtgt ggcaggcggt tcgatggtac gggctaatca gtcaggaatc atcgatgtcg
     3361 gtccacgttc tgcacatatt gccggtctag attatgcagt atttacggag acagaaaaaa
     3421 tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg agaccctgct gattacgtca
     3481 aggtagtgat ggaagacgga gaagaagtga ctattacaaa tacatgtgca gccaatgttc
     3541 ttggattagt gcaagaagaa catttttctt atggaaatgt cccatctgca cgaaaagcca
     3601 tacaggcatt agcagactat tgtcatacga cggttgaaga catcgcagag cagatcatgg
     3661 aaaaatccta tgctaagatt gaaccggtga ttttagaatt ggcagacaag tatcacttgg
     3721 agaaagatca gatttctctt gtaggtgtag ggggcggcgc tgcctcattg attacttact
     3781 ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa cgccgaagtg atttcttcta
     3841 ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg tatcatccct tcgccaagta
     3901 aagaagatat acgatcgttg aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta
     3961 caccagaatc gatcgaagtc cacgtagaaa tcgatccaca tacttcaaaa gtaacggcta
     4021 tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac caaggagatc acaacagaag
     4081 aagcactaga gcttgctgca gaggatatgc gattaaataa aaatgaagtt tgcttattgg
     4141 aaaatacacc tttcttttat gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc
     4201 ttcggatcat cgatcaaaaa ggatttatca aggtccaacg tggacatgct tcatgtatga
     4261 aaacaactgc agccaattat atgacagctg tcgaacaact ttgggaagat atggcagttt
     4321 atcaaactga attgattgcc cgaccagaat tttatctctg cttaggagca agagtctctg
     4381 attttacagc gacggatctg gaacaactac agcttttgat ggatctggaa gtatctacta
     4441 tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat caaacaaaca taagttagaa
     4501 gagttactaa acgaattaca acagatcccc gaagaaattt ggggattcta ccagtttcgg
     4561 cgagatttat tttggaaaaa gattcctttg tccaagcaga aaattcttat acaacaatca
     4621 atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta
     4681 ggagaaatct gtgaacaaat ggctgttcca atcgtttcct gtgaatcaga gcaaataaac
     4741 gaacgaatca cttttgcaac ttatgcagaa gatgaaggga tccgattgat gacggagccg
     4801 ttagaaaaat taaaatgttc aggactagct tcgatctcta aagaaacagc gcaagctttg
     4861 attattggac atgaactatt tcaccacatc gaagcttctg ttaagggaat ttatacgcaa
     4921 agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac atcaatcaaa cattcgtgca
     4981 ttgagcgaga tagcagcgat gagtttttca aaagagatga atcaaagcga tttttcgcct
     5041 tatgtattag aggcagtctt actttggcca tataatgaaa ttcgtagcca agggatctta
     5101 gaagaaataa aagaaataga aaaaagatgt acggagtatg atttagatta caaataaaga
     5161 gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa taaacggtgt tctcctgtca
     5221 actcctcggt cacaagtca
//
LOCUS       DBNIBO010000154         5235 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.160, whole genome shotgun sequence.
ACCESSION   DBNIBO010000154 DBNIBO010000000
VERSION     DBNIBO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.160"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(7..708)
                     /locus_tag="AC1Y77_002403"
     CDS             complement(7..708)
                     /locus_tag="AC1Y77_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1575944.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(816..2259)
                     /locus_tag="AC1Y77_002404"
                     /pseudo
     CDS             complement(816..2259)
                     /locus_tag="AC1Y77_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(2273..4138)
                     /locus_tag="AC1Y77_002405"
     CDS             complement(2273..4138)
                     /locus_tag="AC1Y77_002405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIQ1575945.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(4259..5110)
                     /gene="licT"
                     /locus_tag="AC1Y77_002406"
     CDS             complement(4259..5110)
                     /gene="licT"
                     /locus_tag="AC1Y77_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HIQ1575946.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         1494 a         1191 c          882 g         1668 t
ORIGIN      
        1 ttttatttat gtttcataat caatatggta aggatgcagc tccgtatggt agaccgcctc
       61 cgaatattca atcatttccc caatttgcgt agacgatttc cgtatccgtt tgatgccgat
      121 ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt aaaaatactg ccgagaaatc
      181 atcagaaaat gagtcaatag tatatccttt tttcgtcagc aaacgataca aagattcttt
      241 gccaaattct tctctgctga attgcatcat ttcttgtgga agataatgaa caaaataaat
      301 ataaggctga ttgttcaaat aatataagcg cttaaaacaa acagcttgct caccgaaaaa
      361 ggaaaacaaa ggatcttcct tctttatatg gaccaattgc aaatccagcg tttctttccg
      421 gacttcatat cgggagttct ccaagatttg cgtaaagctt gccgctttgg ataatcgatt
      481 gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt ttctttgcga catattcatc
      541 tgatgccagc agttcgattg cctttcggac agtgattttg ctgacttgaa acagttcttc
      601 taattcactt tctgtcggca acatcgaacc gactggatat tttcctgaga aaatatcttc
      661 tttaatttta tctgcgactt ccaagtatag tacacgtgtc cttttcataa cgtcaccctt
      721 tgctttacta ttactaaaag aatagcacat aaaaaataaa aaaagaacac aaatcacatg
      781 tgagaagtgt gttctttttc attcagctgc tttaattaca agtttacgcc atttgaggca
      841 attacttctt tataccaatc aaaagatttt ttctttgatc gattcagtgt cccatttcct
      901 tcatcgtcta gatccacata gataaagccg taacgtttac tcatttctcc tgttgatgca
      961 ctgacaagat cgatacatcc ccaaggcgta tagcccatca aatctacacc gtctgctact
     1021 gcattagcca tcgcttcgat atgacgacgt aaataatcga tccgataatc atcaataatc
     1081 gtgccatcct cttccatatg atcaatggct ccaagaccgt tttcaacaat gaataatggt
     1141 ttctggtaac gattgtataa ttcattcaat gcgattctca gcccttctgg atcgatctgc
     1201 cagccccatt cacttgcctc aagaaacgga ttttttactc ctcccagcaa gttcccttct
     1261 gaagttgctg cttgtggatc agtctcattg attgccgtag acatataata actaaatccg
     1321 atgtagtcca ctggatattc ttttagcaac tggaagtctt tttccgtttg ctctaatttt
     1381 tccggatcga ctccatattt tttatgggta cgcgctgtgt atgcagggta ttctccacgt
     1441 acttggacat ccgtgcagaa aaaattgaat tcttggtttt ggatcattgt tgctgcctga
     1501 ttgactggat tggcatcgat gctgtaagtt gttgcataga taatcatgca gccgatttgg
     1561 atatccgaac gcaattcgtg gccgattttt acggctttgc tacttgcaac gaattgatta
     1621 tgccatgctt ggaaaatatt ttggtactct cctgctccat tgctttttac taatccttga
     1681 cttaaagctg gaaagtgaaa ggccgaattg atttcattga aagtcatcca atatttgact
     1741 ttactgctgt atctctctaa tactgtctga gcaaatcgtt catagaagtc gatcagtttt
     1801 cgatttttcc agccaccgta ttcttttgcc aaatgaagag gcatttcgta atgagaaatc
     1861 gtgatcacag gttcaatatc atattttaag cactcatcaa tcagctgatc ataaaattct
     1921 aatcccgctt cgttaggagt accttcgtct ccatttggaa aaattcgtgt ccaggcaatc
     1981 gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg caagatcttc tttgaaacga
     2041 tcgtaatgat cgattcctcg atgattcggg taaatatatt tttcttgatc aatcgtccag
     2101 tcaaattctt cactgccgat aatcgcaaag cgctgcttac ctccaggcat agcatctgca
     2161 acggatagtc cttttccgtc tcgatcataa gccccttcta attgatttgg cagcagttgc
     2221 tccaccccat aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct ccttatttta
     2281 caattgccag aacatcttcg tttgcaataa tctctttttg gtctaattca agtatatcgg
     2341 caaatgcatc agtattcgta atgacaactg gagtgattac gctatagcca gctgctctaa
     2401 ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt gacgagatcc ccttgtttga
     2461 ctaagatctc aaagccattc ccatttaatt ctactgtatc catcccgata tgaatcaaaa
     2521 tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa atagtcgtga
     2581 tctctccgtc tgcaggtgca actagttctc ctacttctgg atcaatagcg ataccttttc
     2641 ccatcacgcc agaagaaaaa acttggtctg gtacttcact caatggcagt actttacccg
     2701 ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca ttcgcatgtt
     2761 tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat gtacctacga
     2821 atgcaagaac aaaagcaatt cctgtagcta ttactgctac tgtcacattt gactctacac
     2881 catccaccgt attaatgaaa gtcggaatcg tcaataagct gaccaaactg ctggagaaag
     2941 cttttactcc ggaaaatccg ataatagctc caccaatacc tccagaaata caagcggcaa
     3001 tgaatggttt cttcaaaggt aatgtcacac catacacagt tggttcagtg attccaaata
     3061 acgaagtcac tgtagaagaa atccctaatg ctcgaagttt cgtatctttt gttcgcaatg
     3121 ctacagctaa agcagcccca ccttgtgcca agatggctgg aagaagcatt ggcatcaata
     3181 catcaaaccc atattgttcg atattcaaaa acatgatcgg tacgaatccc caatgcatgc
     3241 cgaacatcac gaatacttgc cataaacttc ccatgattag acctgctacg attggactaa
     3301 atccataaat cgagttgaag agaccgccta aagcattacc ggcaaccgtc ccgattggtc
     3361 ctaaagcaag aaatgttaat ggaaccatca ctactaaaac gacaagcgga actaaaatca
     3421 tttgcagaaa ttgcggaatc acttttttta cgaatggctc taatttactt tgaacccata
     3481 cggctagaat gattggtatg acagaagaag tataaccaga tggtgaaaga atcactggga
     3541 tcccaaagaa actgatagct tctcccgcac cagcaactgc tgtgatagct ggatacacaa
     3601 gtgccatagc aattgctact gctaaaaact ggttggcttt gaacttttta gctgctgtaa
     3661 aagcaagcat gacaggtaag aaggtaaaga caccatctgc tgcagcaaac aatatttgat
     3721 aagtaccgct atcagctgtc agccagccca tcaccgttgc caatgataag aatcctttca
     3781 ataccccggc ggctgccatc gcccctagaa aaggtgtaaa tatactagag ataatatcga
     3841 tcagacgatt caaaatattt ttggactctt cttttccatc ctccgaaact gcaagacctt
     3901 cctcatccac gatcgattga taaacatctg ctacattact gccaatcact acttgatact
     3961 gaccgccact ttgtacgact tggatcacgt ccgggtcttc ttttaacttt tgcgtatctg
     4021 ctaaagattc atcttttaat ctgaatcgca accgcgtagc acaatgtact aaacttttga
     4081 cattcttttg tcctcctacc gcttcccata cacgacgacc aactgcttga ttatccattg
     4141 ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc agattgaaaa aaacagtcac
     4201 aaacattcgt gaacctattt tttcaatcca ggttttgcct gccatacagt aacaatcctt
     4261 atgattggat gctttttttc tcacttacta ctctttgaat atgaatcgtt aaataaatct
     4321 gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag atactcattg atccgttcga
     4381 cacaatgaaa cgcttttgga tactttcctt gaactaatgc gtagagaaat tcatcttgct
     4441 cgtcagatcc ttcccctttg agataacgct gggcaaaaaa ctgcagatga gtaatgatac
     4501 gctgataatt caaagattct tcatcgaaca cgatgccgaa gaatttactg atgatactca
     4561 gtatatctct taccattgtg gtagcggtca tcgtcacttg catgtcttcg tttccttgtt
     4621 ggctattcac aaaatgaaag gcaataaaac cggcttcatc ctcagaaagt tccacacccg
     4681 tcttttgttt cacgaacaaa agagcctctt tcgctgcttg atattctttc ggataaaatt
     4741 tttttgtttc aaataataat ggattaggca gttggatatt ttcttttgct cgtgtgatcg
     4801 cataatgaag atgatcggtc aatgtcagat aaatattagg cgaaaattca acttttagtt
     4861 tatcttcagc cagttcgact acttgtttga cccattcgat ctcctctgcc ggaatatcag
     4921 ccatcagctg ctggaattgg tttttagcaa aagaatctct taaaacaaat tctttttgga
     4981 ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa agctagtcct tttcccatac
     5041 aaacgacttc ttccccgaat ccatttttcg aaatgacgac attgttattc aaaattttat
     5101 caattttcat tttgcttcgc cctctcaaga aaaataaaaa aacctagaat tcgcctactg
     5161 tttcttagct gaattctggt tttgcctgcc gtacagtaac aatccatttc ttattcaaaa
     5221 tcatcatacc atcga
//
LOCUS       DBNIBO010000155         5047 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.161, whole genome shotgun sequence.
ACCESSION   DBNIBO010000155 DBNIBO010000000
VERSION     DBNIBO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.161"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(83..1222)
                     /locus_tag="AC1Y77_002407"
     CDS             complement(83..1222)
                     /locus_tag="AC1Y77_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIQ1575947.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1531..2244)
                     /locus_tag="AC1Y77_002408"
     CDS             complement(1531..2244)
                     /locus_tag="AC1Y77_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIQ1575948.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2498..3199)
                     /gene="nagB"
                     /locus_tag="AC1Y77_002409"
     CDS             complement(2498..3199)
                     /gene="nagB"
                     /locus_tag="AC1Y77_002409"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIQ1575949.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3433..4965
                     /locus_tag="AC1Y77_002410"
     CDS             3433..4965
                     /locus_tag="AC1Y77_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIQ1575950.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
BASE COUNT         1666 a         1012 c          854 g         1515 t
ORIGIN      
        1 attttaattc aaaaattttt cgaaaaataa aaagagttta gcgtacagat attttaaatg
       61 tacactgaac tcttttttat gcttattcac ttgtttcttc cagcactttt ggactgtcta
      121 acttttccga aacatgttcc attcttctca ataaatagat tttccggata tttaatatta
      181 tcgtcttaat cactgcatag acaggtatgc aaatcagcat gcctaatatt cctgcgacac
      241 ttcctgctcc aatcaatacc aagatgatcg tcaaaggatg gatattcatt gatttcccaa
      301 ataacaacgg cttgatcaaa tttccatcta attgttggat cactaaaatc gacaacgcca
      361 tatacaaagc ttgaaatgga gagataaaca aaccgacaat gattgctggt accgctccaa
      421 taaaagggcc gacatacggg ataatattcg tgattccgca aatgaccgca agcaacaagc
      481 tataaggctg atcgaatatt ctcattgcca aaaagctcat tacaccaata atcaaagcat
      541 ctaagatcgt actgctgata taggcagata atgtttcgtt taattctttt accgtttggc
      601 gtaactcttt acgaatcgct ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc
      661 catccttgaa cataaatagc aaaataaatg gaacggtaaa taacaaaata aaaaattgca
      721 tcataaatga aatgattttt gagacacttg cagataaact gactaatatg atattgaaca
      781 aattactaag agataaattt gctgatgcta attgttcttc caaattgaaa tttttaaact
      841 cttcccgctg cgccaattca tttaaccatt tgcttgtttc atctgcatac attggcaagc
      901 tctgggtcaa ttgaattgtc tgttccatta actgaggcac tacattcatg acagttaaaa
      961 caactaaaat gattacgacc aaaaacgtca ataaaaaacc gaacactctc gggatccgat
     1021 gcttttcgag aaaaacaacg attggatcga acatataata gataaaacca gcaacaagca
     1081 atggtaaaaa aattgccgaa agcattttca atattggact aaaaatattc ggcatttgta
     1141 aaataaagta cacgccgatg atcgtgagta ctagctccac tgtccagaaa aataattttg
     1201 attgtttaaa acgtgattgc atacatctcc tccaaggtcc tttgatactt ctattctatc
     1261 atttaacaaa gagttacgcg ctgagatttt catttgctta aaaaaaacaa aaaaaataaa
     1321 catattttaa tgagtagacg agtacttaaa caaaaacacg gtttggagta gtgacaaaaa
     1381 ttatactgca gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa
     1441 gcccagccaa acgaaaaata taaggaacta tttttatttg gctgggctta ttacttgatg
     1501 taggacatct attttacaac ctcagatcat ttattgcgca atactgacta aatgcaactt
     1561 tgtgatattt ttgtgtacgg acaactcctc gatcacatcg gcataagtga gcccttttgg
     1621 aagatcaatc gtatagatat ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa
     1681 attcacatct tcgattacga tatttttctc ttcaaaataa tcggtaagaa aatctttggt
     1741 ttcttttcta tggataaatt ggatttctaa ctttttagtc gtaggtacat ggatcacgcg
     1801 tttcactact gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa tggaatagta
     1861 acccatgccg atggcgatcc ctaatccagc aattgcccat aaagaagcgg cagtcgtaag
     1921 tcccgtcacc gattgtttcg tgacgatgat cgttcccgcg cctaagaaac cgacaccact
     1981 caccacttga gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa gctgtggata
     2041 ctctaaagca tctcttaatg caccactagc gatctcaact tggatcaatg caatgataca
     2101 tgctcccata caaactaaaa tatgcgtacg catacctgct ggacgatttt tatattgtcg
     2161 ttcaaaaccg attgctccgc caaatagcat agcgatccct aaacgtaaaa taatctctgg
     2221 aattgtcaat accatatcat tcatctaaac accctccagt ccaatatatc atgtctgaaa
     2281 ggagcagaca aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt gacaaaattt
     2341 ttgtcatagt cctttttatg aataaacggt gttcaatcag ctgaccctcg gtattactca
     2401 acaaacggca aaatatgggg agctattttt cctttgtctt ctctaatact caggtcaaaa
     2461 cgctgatttc acaacctctt tagcctacta ttgaaaatta caacttactt gctgcttctt
     2521 catcgatgat cacaacaaca ttgtcgtgat tttgcaatac acttgctggc aaatgattcg
     2581 ttacaggtcc ttcaatcatc cctttgattg catcagcttt gctttcgcca aatgccatca
     2641 agacgatttc tttacttttc ataattgaac cgattcccat tgaaacagct tttgttggta
     2701 catcttctgc tttatcgaag aatcgtttgt ttgcattgat tgttgattct gttaagtcaa
     2761 ctactgaagt cgttccgtca aatgaagcac caggttcatt gaaaccgata tgtccatttt
     2821 gcccgattcc taagatttgg atatcgattg gatgtgcatc gatgatttga tcatactttt
     2881 tgcatgctgc ttccagatct tctgcttttc catctggtac aaacgtttct ttgaatggtt
     2941 ttttatcaaa taattggaca tccatgaaat gacggtagct ttgttcatct gaaccgccta
     3001 gtccaacgta ctcatctaag tttacagaag tcatttcact gaaatccaaa tcgcttgaag
     3061 tcatttcttt atataaagta actggtgtgc ttcctgttgc caatcctaat acttttgcac
     3121 ctttgttcat accttctttg atgatttcaa atgctttttt tccgccttct tcagcgtttt
     3181 ttacacgaat gatttccata gtgattgctc ctttatattt tggtatagcc caattatagc
     3241 attctttttt tctttttaca ataattagta tagaccaaat tgcatttttt tcattttctt
     3301 gacaataaat aggaatccgt gttaggtttc ataagatggt tcaatcaaac aatccatgtt
     3361 gcatgaaaac caagcaagtg attcactgtg gacgctttat gtctgcagtt tttttatgaa
     3421 aaaggagtta ttatgagtat actgacatta aacaacatta cccagcaatt tggcgacaaa
     3481 atcctctatg aaggtgtcga tctgcaatta aatgctggcg aacatcttgg gctgattgga
     3541 caaaacggag ccggtaaaag tacattgatt aaaatcatca ctggtgagat cttaccagat
     3601 gacggacaga tccattggca aaaaaatatt cacaaaggtt acctcgatca atatgtggaa
     3661 gtcgatgaaa cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa
     3721 aaggaagaaa aaattgcttt tctttaccaa acatatagcg agaccttcga tgattccttg
     3781 ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac
     3841 accttagttg ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg
     3901 ttgaaaaatc taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa
     3961 aacccagatg ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa
     4021 tggctgacgc agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa
     4081 gaatttcttg atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag
     4141 tatactggcc atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg
     4201 cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac
     4261 aaagccggtt cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa
     4321 cggctgacgc ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt
     4381 gtcacgacat tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt
     4441 gcacccatca atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg
     4501 attggaaaat ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat
     4561 ttccacttcc cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat
     4621 ccattagaaa caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa
     4681 ttacgtcgcc aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact
     4741 acattaagtg gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt
     4801 aatttactga ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta
     4861 caagacggga tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt
     4921 tatcaagatc ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata
     4981 gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt
     5041 ggccaat
//
LOCUS       DBNIBO010000156         4982 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.163, whole genome shotgun sequence.
ACCESSION   DBNIBO010000156 DBNIBO010000000
VERSION     DBNIBO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.163"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            83..844
                     /locus_tag="AC1Y77_002411"
     CDS             83..844
                     /locus_tag="AC1Y77_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1575951.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            1786..2079
                     /locus_tag="AC1Y77_002412"
                     /pseudo
     CDS             1786..2079
                     /locus_tag="AC1Y77_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            2751..>4982
                     /locus_tag="AC1Y77_002413"
     CDS             2751..>4982
                     /locus_tag="AC1Y77_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIQ1575952.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         1757 a          712 c         1057 g         1456 t
ORIGIN      
        1 cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa
       61 actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact
      121 atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag
      181 tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga
      241 tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa
      301 aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact
      361 ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat
      421 gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc
      481 aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg
      541 gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag
      601 tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg
      661 tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag
      721 agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac
      781 gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa
      841 ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa
      901 ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt
      961 tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat
     1021 acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata
     1081 attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta
     1141 tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa atacattgtt
     1201 gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg aataaacata
     1261 ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata ctaccgatag
     1321 catttttttg cttataaaat aattataagt atttagagat ttttcttcta acattcttca
     1381 aacaagaaaa atcacaaagg ttattgttat ttttatatat tctttttaag aatgattgtt
     1441 tttttataga aattttaaaa aataaaacac gttattgatg gatagttagt ttgttatttg
     1501 gtaatattat agaagttgac tggtagtgat ttatttttcg tcgttttata aaaatgttat
     1561 tttttggaaa aattcacaaa tatgtaacga atgtttttct ggaatttgct gtgttaaaaa
     1621 gcagtatgac taatataaat actattgtca tttatgcaat tttttatgga tagtaatttt
     1681 agaggggaat caatgaagga agaatagaag ggaaaaatag ttgcaaatac gccagttagc
     1741 aaaaatagca atacatcttc aagctttaaa attcgagaga atcatcttgg tagtggaatc
     1801 aactacaaca aaaggattaa accaattaat agatagaatt accaatggaa aaatagaaaa
     1861 ggtagttatt ctatacaaaa gttgtttaat tcgcttcggt tatgaactta gtgaaaccct
     1921 ttgcaatata tggtacagaa tatagaaatt attgacatta cagaaaatga tgaagattta
     1981 atacagattg tcactatttg tagatacctt ttacaaggaa accgagccaa taaagctaag
     2041 aagatgatta aggagttgat ggaagacgat acaagttaag aaagtgaaac tacgcccaaa
     2101 taaagagcaa aaagagtaat tggacgtgga aaatgcaaga aatgaaccat cgattcggtg
     2161 gacagtttat tagtaatgac ggtttgagaa catccatgac gagaatgaag gaacatccta
     2221 aatgcgttta gttgaaccaa gtgccaaata atatggccaa ataagcgatc agttatcgat
     2281 gaatcccaag tattctaaac tatatattca ttttgatgct aagcattgat atattttttt
     2341 gggattgatg aaggttaacc aatacaggga ctcactgatg aaattctaga gtctgacctt
     2401 ggtatcaacg atttagtgat tgtttctaat ggaaaagtat aaaagtatat aagtacatta
     2461 acaaaataac tattagggtt acgctccagc ttgtgcaagg cactggcagg aggaatcgtg
     2521 aaatcataat tatggttaag ccacacggac aactaaaagt catacaaagc tatcgaatca
     2581 atcaacagaa gacaatcggc aatcattagc tttgtttaca gcttacattg caacgcttga
     2641 gttgaaccga aaatacgagg gaggtaacgc agccattttg agttggggaa tctttttaga
     2701 aaattatcgg gtaccttgtt gtttagtcaa aattataagg gggagtaata gtgaggaaca
     2761 aatttcataa actgttaagt atgtttctag ttatgcttat tgtgatcaat caatttggga
     2821 acataacggt tctagctgag tctttagcag aacagccaga gcgttcgctg acgatcgaag
     2881 ttgatcaaga ggaggctgct ttgggtgagc agttccagtt gcgagtcaga ggaacaccag
     2941 acctgctaaa tgcattggtc attgataaga gtgaggattt gattgaaggg gaagtgaagc
     3001 aactcaatga ttacgaacgt ctgattactt attcttccca gcattcaggt aaatacgaat
     3061 tgaccgcaag aagcggtgat attcaatcaa atacggtctc aatagaaatc agtgtaccag
     3121 aagaaagcac aagtgagtct tttaatgtac cagaagaaag cacaagtgaa tcttttaata
     3181 taccagaaga aagcttaagt gaatctttta atgaagccag atcatctgag ccatctataa
     3241 cgcaggaatc tcagattgaa agacaagagg agctcttgaa ttatgacatc ggcatctctg
     3301 ctgagcgccc cactttgccg agcaggcata cacaaacgtt aaagggatac attcaaggag
     3361 gcgctggcga tattcttgaa gaatatgggg agatggtcgt acgagttcct agagcatcga
     3421 ttggcttaat tgagttcatt agagtggagg tcgctgatga ttcaccttta aagccactgg
     3481 cattttacca agaaggcaca gacgttgttt ttccatttac ctttgattat gaaggattaa
     3541 atgaagccgc tcagtcgtat acattcagta tcacgtttga agttgctttc tttcaatcag
     3601 gcggtactga gccaaataat gactttcaag tgagtcttac ttatgctgag cgagcagcaa
     3661 acacggaatt ttcaattacg cctattcctt catcaagacc cattttttcg aaattatact
     3721 atggctctag agaaatcgac agccaaggaa atcttgggaa tctgctgatt gacgggcaag
     3781 acgtgaccaa cagtaataaa tttattttgc aggtaaatta tgaaggatca cgaaaagacg
     3841 gtgtagtggt taccgatgta ctaccgcaag gaacaacttt ggcaccgaat ccgactacat
     3901 ggagttccac tggaaataga gaacctattt caggtggggt tcggatcgca gaagtaacga
     3961 atccaggtga agcaaacgtt agaactcatt atgtttccga ggaatttgtg gacaaagtat
     4021 cttttaataa ggaaacgaat acacttgttg tggactttgg taatctagca gagggtcaat
     4081 cgtattttat tgaatatgct cttgaagtga ccgatgtcga cttgttcaat gaaggaagac
     4141 aagaggatgg gttttctcgg aatcgagcga gtatgaccta caataatcag gtaatgagcc
     4201 gagactaccg cttgagaatc cctgaaagta atcttccggc aatctcttta agaaaaagtg
     4261 tggacaaaaa tgttatcaat ctcaatgaaa atgaactaac gtatactctg catgtctcga
     4321 tgttttctgg aacaagaaat acgcttgtta ttgaagatgt tttggaagaa ggcatggaat
     4381 ttgtgtcatt tctatcagat attccctctt tcattgatgc cgaagtcaat aatggaatag
     4441 ttcgtttcag tgcgaataat acgattggtg gtccgacaag ttttaccctc tcttttaagg
     4501 tcaatgtaga ggcgtatgcg gtcggcgatc aaattttaaa ctttgcaacg cttgtgaata
     4561 gtgacaatcg attgaatacg agtactgtaa gaacaaggaa aatcgatggg cgtattaaag
     4621 taattaaaac tgatgagaat ggccaaaggc tggcaggagc aaaatttgaa attttatcaa
     4681 gcgattcaca agtgatacaa gaaggtataa cggcagaaaa cggagaattt ttaagtgaac
     4741 cgcttcctgt aggtagctat caagtccgag aaacagaagc accggaaggg tatcttttag
     4801 atgcgacggt acatgatgtt tttataaatg aatactccat agaagaagtg acgttaacca
     4861 ttgaaaacat ccttgatacg ggtggtgttg aactaattaa acatgctgaa ggcagtggtg
     4921 aagtccttca aggagctgtt tttgagttac agaaccgaga aggagaaacg ctacaaaccg
     4981 gt
//
LOCUS       DBNIBO010000157         4921 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.164, whole genome shotgun sequence.
ACCESSION   DBNIBO010000157 DBNIBO010000000
VERSION     DBNIBO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.164"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..164
                     /locus_tag="AC1Y77_002414"
     CDS             <1..164
                     /locus_tag="AC1Y77_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575953.1"
                     /translation="ARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPD
                     GEEIKISNE"
     gene            179..1048
                     /locus_tag="AC1Y77_002415"
     CDS             179..1048
                     /locus_tag="AC1Y77_002415"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIQ1575954.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1029..1763
                     /locus_tag="AC1Y77_002416"
     CDS             1029..1763
                     /locus_tag="AC1Y77_002416"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1575955.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1796..2704
                     /locus_tag="AC1Y77_002417"
     CDS             1796..2704
                     /locus_tag="AC1Y77_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HIQ1575956.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2701..3243
                     /gene="sat4"
                     /locus_tag="AC1Y77_002418"
     CDS             2701..3243
                     /gene="sat4"
                     /locus_tag="AC1Y77_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HIQ1575957.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3336..4130
                     /locus_tag="AC1Y77_002419"
                     /note="aph(3')-IIIa"
     CDS             3336..4130
                     /locus_tag="AC1Y77_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HIQ1575958.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4406..>4921
                     /locus_tag="AC1Y77_002420"
     CDS             4406..>4921
                     /locus_tag="AC1Y77_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1575959.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         1685 a          740 c         1124 g         1372 t
ORIGIN      
        1 ttgctagacg tattggccag actccacaga atttcaataa aaaattacaa cgagaaacag
       61 taaccttgga tgagttgaag gccattgctg atgtgctagg tgtcaagttt gtgcaggcat
      121 ttattttacc agatggagaa gaaataaaaa tatctaacga ataaaggagg atgtttatat
      181 ggtaggtaat gttattcaaa tagtaacaga gaaattatca tctttgcctt ttatagaagg
      241 catcgtatta gggggctcac gtgcaagagg cacccataca gaaaattctg atatagatat
      301 cggaatctat tacaattcag actcatttga cctgacagcg attaatcaaa ttgctacaga
      361 gttggatgat gagaatagaa acaaccttgt tgtacctccc ggagcgtggg gtgattgggt
      421 taatggcggt ggatggttag ttataaacgg gtgtcatgtt gacttgatat tacgtgatat
      481 aaaacgggtg gaacaaataa tcaaagatac ggagcaagga attgttactg ccaattatca
      541 gaccgggcat ccccatggtt atataagtgc tatgtatcgt ggagaattag cgattagtaa
      601 aatactatat gctaagaacg aaagcttatg cgaattaaaa aagcaggcag aaatttaccc
      661 tactgctcta aagaaaagct tgatgaactt ttttatattt gaagctgaat tctctttaat
      721 gtttgtaaaa gcaaatgcgg gggcagagga taaatattat attgcaggcc atgtttttcg
      781 tattatttca tgcttaaatc aagtactatt tgcatgtaat aatgcttatt gcattaacga
      841 aaagaaagct ataaaactgc ttgaaacttt tgaatataaa cctgaaaaat atgccgaaag
      901 agttaatcat atttttgaag tactcggtct ttctcttttt gaatgctacg acatgaccga
      961 gaaactttat aaagaagtga atgaaattgt atcggagata aataactttt taaacgagga
     1021 gagttcagat gaaagaaaac aaatatgatg ataatatatt ttttcaaaaa tacagtcaaa
     1081 tgagtcgctc gcagaaagga ctggctggtg cgggagaatg ggagactttg aaaaagatgc
     1141 tacctgattt taagggtaag cgtgtgcttg atttaggatg cggctatgga tggcactgta
     1201 tatatgcgat ggaaaacggt gcttcctctg tagtaggtgt tgatatttct cataaaatgc
     1261 tcgaagtagc aaaaggaaaa acccattttc cacagattga atatgaatgc tgtgccatag
     1321 aagatgtgga tttcccagag gagagctttg atgtaatact aagttcgctt gcgtttcatt
     1381 atgtagcaga ctatgagaat ttaataaaaa agatatatag gatgctgaag gctggtggca
     1441 atttagtttt tacagttgaa catcctgttt ttactgctca tggaacacaa gactggtatt
     1501 ataacgaaaa aggagaaata ctgcatttcc cggtggacaa ttattattat gagggcaaac
     1561 ggacagctat gtttttggaa gaaaaggtta caaaatatca tagaacactg accacatatc
     1621 taaatacact gctttcaaat agttttataa taaatcagat tgtggagcca cagccgccag
     1681 agaacatgat ggatattccg gggatggcgg atgaaatgcg acgcccaatg atgctgattg
     1741 tatcggcaaa aaagaagatg taataatata gaaaaaataa acgaggagta tgtaaatgag
     1801 atcagaaaaa gaaatgatgg atttagtact ttctttagca gaacaggatg aacgtattcg
     1861 aattgtgacc cttgaggggt cacgcgcaaa tattaatata cctaaagatg aatttcagga
     1921 ttatgatatt acatattttg taagtgatat agaaccgttt atatctaatg atgactggct
     1981 taatcaattt gggaatataa taatgatgca aaagccggag gatatggaat tattcccacc
     2041 tgaagaaaag ggattttcct atcttatgct atttgatgat tacaataaaa ttgatcttac
     2101 cttattgccc ttggaagagt tagataatta cctaaagggc gataaattaa taaaggttct
     2161 aattgataaa gattgtagaa ttaaaaggga catagttccg actgatatag attatcatgt
     2221 aagaaagcca agcgcaaggg agtatgatga ttgctgcaat gaattttgga atgtaacacc
     2281 ttatgttatt aaaggattgt gccgtaagga aattttattt gctattgatc attttaatca
     2341 gattgttcgc catgagctgc tgagaatgat atcatggaag gtcggcatcg aaacaggctt
     2401 taaattaagt gtaggcaaga actataagtt tattgaaagg tatatatccg aggatttgtg
     2461 ggagaaactt ttgtccacct accggatgga ttcctatgaa aacatatggg aagcattatt
     2521 tctatgccat caattgttca gggcggtatc cggtgaggtg gcggaaaggc ttcattatgc
     2581 ctatccggag tatgatagga atataacaaa atataccagg gacatgtata aaaaatacac
     2641 tggtaaaacc ggctgcctgg atagcacata tgccgctgat atagaagaga ggcgggaaca
     2701 gtgattacag aaatgaaagc agggcacctg aaagatatcg ataaacccag cgaaccattt
     2761 gaggtgatag gtaagattat accgaggtat gaaaacgaga attggacctt tacagaatta
     2821 ctctatgaag cgccatattt aaaaagctac caagacgaag aggatgaaga ggatgaggag
     2881 gcagattgcc ttgaatatat tgacaatact gataagataa tatatcttta ctaccaagac
     2941 gataaatgcg tcggaaaagt taaactgcga aaaaattgga accggtacgc ttatatagaa
     3001 gatatcgccg tatgtaagga tttcaggggg caaggcatag gcagcgcgct tatcaatata
     3061 tctatagaat gggcaaagca taaaaacttg catggactaa tgcttgaaac ccaggacaat
     3121 aaccttatag cttgtaaatt ctatcataat tgtggtttca aaatcggctc cgtcgatact
     3181 atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt
     3241 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     3301 tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg
     3361 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     3421 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     3481 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     3541 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     3601 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     3661 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     3721 attgtcccta tacgaatagc ttagacagcc gattagccga attggattac ttactgaata
     3781 acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc
     3841 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     3901 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     3961 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
     4021 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     4081 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagatt
     4141 tagatgtcta aaaagcttta actacaagct ttttagacat ctaatctttt ctgaagtaca
     4201 tccgcaactg tccatactct gatgttttat atcttttcta aaagttcgct agatagagtt
     4261 ctatattaga gatgtaaaga gttttggtga agaggtttgt gagtatggct tttataacca
     4321 gggtgcaatg agaagcacta acagcagcta gcttaagaac gcataggata tactttttat
     4381 ggaagtcaca gaggaggaat aaaatttgaa caggattaac agagtaacct ctattcttat
     4441 tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt gcacagcgat ttaatataag
     4501 cttaaggaca gtttacagag atatccggac acttgaagaa gccggaatac caattggatc
     4561 tgaggccgga aaaggatatt ttcttgtaga gggcttccta cttccgccgg taatgtttac
     4621 agcggcggaa gtgggtgcat tgattacagc agggaaattt ttaaattgcc atggagatga
     4681 atcatttata aaggattttg attcagctat gtataaaatc aaatctattt tgaagcatgg
     4741 tgaaaaaaac tatgcacagg agttggagaa cagtattaac gtgtacagca catctggaca
     4801 gaaaaataca ttggcggata acgttattgc agcaattcag actgcaatct gcaataaaag
     4861 ggtaatatca attcaatatc ctgcatcagg agggcaagaa ccggaaagca gaatgataga
     4921 g
//
LOCUS       DBNIBO010000158         4734 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.165, whole genome shotgun sequence.
ACCESSION   DBNIBO010000158 DBNIBO010000000
VERSION     DBNIBO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.165"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            25..696
                     /locus_tag="AC1Y77_002421"
     CDS             25..696
                     /locus_tag="AC1Y77_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIQ1575960.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="AC1Y77_002422"
     CDS             755..1195
                     /locus_tag="AC1Y77_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575961.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="AC1Y77_002423"
     CDS             1207..1875
                     /locus_tag="AC1Y77_002423"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIQ1575962.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="AC1Y77_002424"
     CDS             1939..2562
                     /locus_tag="AC1Y77_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIQ1575963.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="AC1Y77_002425"
     CDS             2618..3004
                     /locus_tag="AC1Y77_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HIQ1575964.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="AC1Y77_002426"
     CDS             3217..4608
                     /locus_tag="AC1Y77_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HIQ1575965.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1577 a          782 c         1007 g         1368 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaa
//
LOCUS       DBNIBO010000159         4715 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.166, whole genome shotgun sequence.
ACCESSION   DBNIBO010000159 DBNIBO010000000
VERSION     DBNIBO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.166"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            263..691
                     /gene="rplM"
                     /locus_tag="AC1Y77_002427"
     CDS             263..691
                     /gene="rplM"
                     /locus_tag="AC1Y77_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIQ1575966.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            707..1099
                     /gene="rpsI"
                     /locus_tag="AC1Y77_002428"
     CDS             707..1099
                     /gene="rpsI"
                     /locus_tag="AC1Y77_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIQ1575967.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1188..2354)
                     /locus_tag="AC1Y77_002429"
     CDS             complement(1188..2354)
                     /locus_tag="AC1Y77_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1575968.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2409..3011)
                     /locus_tag="AC1Y77_002430"
     CDS             complement(2409..3011)
                     /locus_tag="AC1Y77_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575969.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            3134..3334
                     /locus_tag="AC1Y77_002431"
     CDS             3134..3334
                     /locus_tag="AC1Y77_002431"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1575970.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            3346..3672
                     /locus_tag="AC1Y77_002432"
     CDS             3346..3672
                     /locus_tag="AC1Y77_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575971.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            3825..>4715
                     /locus_tag="AC1Y77_002433"
     CDS             3825..>4715
                     /locus_tag="AC1Y77_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIQ1575972.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVD"
BASE COUNT         1660 a          710 c          810 g         1535 t
ORIGIN      
        1 aataaggggt tgtgacaaga cttttgtcac agcctctttt tgtttttcat tttgacagga
       61 aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt tcatataaat ttttcaatca
      121 agcagaaaaa cgttgacatt gactagatta gcgggtaata tgttaaatgg tattgtttgc
      181 cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca tcagaactta aattggagga
      241 aaataacgtg cgtacaacat atatggccaa accaggcgaa gtagaacgta aatggtatgt
      301 agtagacgca actgatgttc cattgggacg tctttcaaca gttgttgcat ctgtactacg
      361 tggtaaaaat aaaccaactt tcacacctca tttggatact ggcgatttcg tcatcgtcat
      421 caatgcagac aaagtgaaat taacaggtaa aaaagcgact gacaaaattt attaccgtca
      481 cagcaactac ccaggaggat tgaaatcaat cactgctggc gaattgcgtg ctaaaaattc
      541 tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct aaaaacactt taggccgcaa
      601 acaatttaca aaattgaacg tatacggtgg agcagaacat ccacatgcag cacaacaacc
      661 agaagtttta gacatcacaa acttaatcta ataggaggga atttcattgg cacaagttca
      721 atatatcggc acaggccgtc gtaaaaacgc agttgctcgc gtacgtctag taccaggaac
      781 tggcaaaatt actgtaaata aaaaagatgt tgaagaatac attccacatg ctgacttgcg
      841 tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa ggcgcatatg acgtatttgt
      901 taacgtaaac ggcggaggat acgctggaca atcaggagct atccgtcatg gtatcgctcg
      961 tgcattgcta gaagttgatc ctgacttccg tttagctctt aaacgtgctg gcttacttac
     1021 tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt aagaaagcac gtaaagcttc
     1081 acagttttca aaacgttaat cgttgttata ccaacgattt cagacacttt ctcattgcga
     1141 gaaggtgtct ttttttcgtt ctggtaatga gaaaggtaat gacaatatta taaaccagtg
     1201 taagcaatga atttttctaa cgcatttcgt tgagcttttt cagtaacatg agaataagtg
     1261 ttaagggtag tttcagcatt tttatgaccc aatcgatctt gtgtttcttt gatacttgct
     1321 ccaccttcta gtagaagagt agcgtgagtg tgccggaatc catgaatagt aacgtgctta
     1381 aatttatatt tcttctctaa acgtttcaat atattatttg aatagtccgc atgcaaaggt
     1441 tgattaatgt caccgtctgc atttacatag gtaaagataa gttgattttt tgtatttgta
     1501 tttatgccaa actgcaaaag ctctcgagct tgttcttttt tccagatttt taacatttca
     1561 atagtttgtg catcaattgg taatattcgt tcggaagata tattttttgt atttttttga
     1621 atcctgacag tttttcttct tgcattcttc tcttcatttc gaatagttgc atagctcttt
     1681 ctaacataca ttagctgttc ttcaaaataa atgtcagacc aatgaagagc agctagctct
     1741 cctttacgta taccagtagt aatcaaaagc tggaagatta aaatatgttt taaaggctct
     1801 tctttattga gaatatccca aaaagctttt agttcttcta atgaaaagta attttcattt
     1861 tctttctttt cccgctttgg tagatttgta tttgccatag gatttcggtg taatagctct
     1921 gcaaaaatgg catactcaaa tatttgagag ctgtaagact taagctgttt gatattcttg
     1981 aattttaaag cccattcatt gactgcattc tgacagtgaa caggagtaat cttagaaatg
     2041 gacttattcc cgaattttgg tagtatatgc aagcggaaaa ggtcttttgt acgttgatat
     2101 gtcgtttctt gtacacgatt cctataagaa ggcaaccatt cttcatacac tgttataaac
     2161 aagtcattag aaggcttatg atagccggta tcttcaatct cgaccttttt tcttgcttcg
     2221 tgtaacttgg cttctttttc agttttaaac ccttttttta catagtattc ttgctttcca
     2281 ttttcatctt tacgaacata caagcggaca ctatatcgtg caccgctttt tgttgtgtac
     2341 ttttgtattg acataactaa tctctccaat ctgataactt ggcgagttgg ctgattgaat
     2401 aattagtttt attgaagttt tgatattaat cgagctgtta tctctctttt tagattgtta
     2461 acctgtactt gggttaagtt gccaaataac tcagattcta actcttttct ttcatcgata
     2521 tttaagttta aagctttatt aataaacttc ttttgtttgg caaatagaat cgtgtattca
     2581 aaggagtcct ctagaatact ttttaaaaaa agaaagtcgg agtcatcttc ttgaagtttg
     2641 acttcaagta aagaagtcat ggaaaagtat agattagata attcagtaaa ataatctgag
     2701 tcaagactgg caatattctt gactattttg tccttttttt ctaaacgagc agtttttatt
     2761 ctatcaagat taggttgttc tatggaattt gtttcgtcta atcccatcaa ataacttaca
     2821 ggaacatgga aataatcagc aaggatattc catgtttcag tatttctagg atttcgctca
     2881 ccacggtaat agttcgtaag agttgtatag ttaataccag tttctgtact aatttgttta
     2941 atggtctttt tactggaatc aatataaaat tttattctat tcaaagtaac gccccctata
     3001 gtaaattcca aactattata gcatgaaaac cacaaattga gtaatatttt aattgatatt
     3061 actcgttttg tggtttaaaa tgtgcttatt aagataaaaa ccacaaagtg aataatttga
     3121 aaggaggcgc tgaatgataa ctcttaatga tcaattcatt agaagcttaa gaagacatag
     3181 agccgatctt atacttacta aaaacgatgc agcaaaattg ataggtataa atcggaaaac
     3241 atatgtcaaa atcgaaaatg ggagcaaaga aagtattaga gcatctactt atcaaaagtt
     3301 ggtaaattgg ttacttaatg atttgaaaat ctaaataaat acaacttggc gagttggctg
     3361 attgaagaaa tagaaaatga gaggagaaaa ataatggatg caggaattac agtaatgtta
     3421 gataaacaac aaacgaatca attgaaaaat tatgtttttg aaatgacaaa agaggctatt
     3481 gatcaagcaa gaattgatac cggattagaa agaccatttc taaaaggaaa agagatggct
     3541 aaatatttga atgtatcata tacaactttt ttgaaattta aaagaatggg attgccagtt
     3601 attttattag aaaaaatgga gttgttttca aaagaagaat gcaaaaaatg gatattgagt
     3661 catcaaattt aaaaaacacc tcgtttttta aatttgatga agtagaaaaa attaaaaaga
     3721 gtgttcccgt cagtttgcag atcgggggaa cactcaataa taaagttctg agaaaacttt
     3781 atttgaatct attatagcaa ataaaatcag aagcaggtgt gacaatgaat gaaaaagaag
     3841 ttgaaagctt agttgaaaag caaggctatt ctaattctga tatatatgca gaattatact
     3901 atcaatatcc tattttttta aacgtaaatg aaaaatataa gaaaatgaaa ctaagttcta
     3961 agttagttta tatgttacta aaagatagaa atagactttc attgaaaaat aacttgtatg
     4021 atgagaatca aaaaattttt ttgatatttg aaatcaaaaa tctcgtagaa atttcgggaa
     4081 tgtcaaaaaa tacaatctta tcagcactag atgaacttca aaaagttaaa cttcttgtga
     4141 aaagaaaaaa tggcttcaat aaaagtaaaa tgcaaatgtt cccaaatttt tattatttgt
     4201 taaagcctgt tctgtgcaaa gatgatgttt ataaaattaa taagataacc catcaagaaa
     4261 atagacaaaa aaacttgata gttcaaaatt tgaactatgg aacaaatcaa tattctgttg
     4321 ataattcaaa atttgaacta tgcgacacat caatgaaaac aagagacaaa aacaattttg
     4381 ctgatagttc aaaaaatgaa ctagaattat ataagtctta taaagatagt aaagatatta
     4441 aagaatcaca taatgatgat cttcttgaat cactttcaac taaaaataat aaattagatg
     4501 aagaattgat cgaaacattt attgaagaaa atcttcttaa acaaatatgg ggagaaagta
     4561 ttgtgaattg cttaaaactg gctagcaata gtgattatcg tcaattcgac aactggtaca
     4621 aaaaattcaa atatgcgatt agatctgctg aaaaagaaca aaaaattcaa ctgaaaatta
     4681 tctatgaaat ttgtaataac aagtattttg tagat
//
LOCUS       DBNIBO010000160         4683 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.167, whole genome shotgun sequence.
ACCESSION   DBNIBO010000160 DBNIBO010000000
VERSION     DBNIBO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.167"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            5..862
                     /locus_tag="AC1Y77_002434"
     CDS             5..862
                     /locus_tag="AC1Y77_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIQ1575973.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            884..1393
                     /locus_tag="AC1Y77_002435"
     CDS             884..1393
                     /locus_tag="AC1Y77_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIQ1575974.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1430..1831
                     /locus_tag="AC1Y77_002436"
     CDS             1430..1831
                     /locus_tag="AC1Y77_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575975.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1943..2296
                     /locus_tag="AC1Y77_002437"
     CDS             1943..2296
                     /locus_tag="AC1Y77_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575976.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2330..2773
                     /locus_tag="AC1Y77_002438"
     CDS             2330..2773
                     /locus_tag="AC1Y77_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1575977.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2790..3875
                     /gene="uxuA"
                     /locus_tag="AC1Y77_002439"
     CDS             2790..3875
                     /gene="uxuA"
                     /locus_tag="AC1Y77_002439"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIQ1575978.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3868..4581
                     /locus_tag="AC1Y77_002440"
     CDS             3868..4581
                     /locus_tag="AC1Y77_002440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIQ1575979.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         1620 a          712 c         1060 g         1291 t
ORIGIN      
        1 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
       61 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
      121 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
      181 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
      241 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
      301 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
      361 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
      421 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
      481 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
      541 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
      601 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
      661 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
      721 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
      781 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
      841 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
      901 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
      961 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     1021 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     1081 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     1141 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     1201 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     1261 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     1321 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     1381 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     1441 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     1501 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     1561 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     1621 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     1681 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     1741 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     1801 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     1861 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     1921 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     1981 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     2041 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     2101 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     2161 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     2221 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     2281 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     2341 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     2401 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     2461 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     2521 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     2581 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     2641 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     2701 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     2761 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     2821 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     2881 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     2941 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     3001 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     3061 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     3121 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     3181 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     3241 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     3301 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     3361 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     3421 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     3481 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
     3541 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
     3601 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
     3661 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
     3721 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
     3781 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
     3841 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
     3901 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
     3961 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
     4021 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
     4081 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
     4141 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
     4201 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
     4261 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
     4321 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
     4381 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
     4441 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
     4501 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
     4561 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
     4621 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
     4681 ttt
//
LOCUS       DBNIBO010000161         4683 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.168, whole genome shotgun sequence.
ACCESSION   DBNIBO010000161 DBNIBO010000000
VERSION     DBNIBO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.168"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            394..1527
                     /locus_tag="AC1Y77_002441"
     CDS             394..1527
                     /locus_tag="AC1Y77_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIQ1575980.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1558..1878
                     /locus_tag="AC1Y77_002442"
     CDS             1558..1878
                     /locus_tag="AC1Y77_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HIQ1575981.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1907..2371)
                     /locus_tag="AC1Y77_002443"
     CDS             complement(1907..2371)
                     /locus_tag="AC1Y77_002443"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1575982.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2502..3755)
                     /locus_tag="AC1Y77_002444"
     CDS             complement(2502..3755)
                     /locus_tag="AC1Y77_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIQ1575983.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3784..4101)
                     /locus_tag="AC1Y77_002445"
     CDS             complement(3784..4101)
                     /locus_tag="AC1Y77_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIQ1575984.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4127..4447)
                     /locus_tag="AC1Y77_002446"
     CDS             complement(4127..4447)
                     /locus_tag="AC1Y77_002446"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIQ1575985.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         1489 a          944 c          839 g         1411 t
ORIGIN      
        1 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
       61 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
      121 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
      181 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
      241 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
      301 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
      361 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
      421 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
      481 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
      541 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
      601 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
      661 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
      721 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
      781 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
      841 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
      901 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
      961 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
     1021 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
     1081 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
     1141 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
     1201 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
     1261 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
     1321 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
     1381 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
     1441 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
     1501 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
     1561 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
     1621 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
     1681 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
     1741 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
     1801 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
     1861 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
     1921 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
     1981 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
     2041 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
     2101 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
     2161 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
     2221 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
     2281 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
     2341 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
     2401 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
     2461 aaaaacggaa aacaatcact tgttctccgt tttttgatta atcaagcatc taaagcttct
     2521 tttcttcttt cttggatcgt agctaccttg acgaacggta gatagatcaa aatagaaaga
     2581 accaaattga cagcggctag tacaccacct gcaatacttt ttgttgccaa tacaccgcca
     2641 ataattggtg gagttaccca tggaggcata acggtagctg ctggcactaa acctgtagaa
     2701 gtcgcaaagt aagcaacact aactaagacc attggtgtca aaataaatgg aatgaacatg
     2761 atcgggttca acacgatagg taacccaaac atcgttggtt cattaatatt gaagacacct
     2821 ggcgcaatcg acaagtttgt tacaaccata tatggtttgt ttttacgtcc tactaaataa
     2881 atagctaaca gtaaacctaa agttgcacct gttcctccta agttgacaaa gctgtcaaag
     2941 aaaggtttgt tcacgataaa cggtgcgcta tgcccagctg cgattgcatt aacgtttgct
     3001 tcaatggctg gcgcattgat tgtttgcatc aaaggatcga ccatattcgc tccgtgtaaa
     3061 ccaaagaacc ataagaatgg tgtgataaat gcaagcagta aggcagatgg ataagagttt
     3121 gcaagtccca taaatggttc ttggaccact gtatagaaag aacctacgat atcatcaaca
     3181 ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag agatagtgat cattgctggt
     3241 agtaatgaag caaatgcttt tgcaactgcc ggtggcacac catcaggcat tttgatgatc
     3301 aatttttcat ttccgcttaa acgtacaaag atttctgttg aaataatccc aacaataata
     3361 gcaataaaca aaccgtttga gctcatacct aatgctccgc ctaaagcgaa gaaagagcct
     3421 aaagaaatca cacctgcgga aacaccgtct tttcggtagc cgttagctaa atgataagca
     3481 agcaagaaag caatcaagac agaaagaatg gcaaatgtac cgttccaagc agcagccccg
     3541 aatcctttcc atgattcacc accaaaaatt gagttcatca attcttggaa cccgggaatc
     3601 ggtaagttat tgatcattac tgctaatgct cctaagatca ttaacggcat actcaccata
     3661 aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa tttttgatgc tacaggtata
     3721 aaatgtttct ccataaatgc aacaaattta tccataaatc ttccctcttt tctttactgt
     3781 agattacgcg ttcaatcgac gatacagatc aatgatctct tttgctaaat ctgtaaaagc
     3841 tatagatgtc atcagatgat cttgactgtg gaccgtcaaa agcgtgactt caacgtgatt
     3901 tccttgtgct tcttgtgtca gcatctctgt ttgagagtga tgtgcttgga ctaaagattc
     3961 ctctgcatct ttgatttttt catctgctaa agtgaaatca ccttttttcg cagcagcaat
     4021 cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg atcagaccca tgatggcttc
     4081 tagatttttt tgatcttcca tttattgtat cctccgataa tttttattat ttgtttatta
     4141 gtttttctgc ttggtcgagt actttttcac cattcatcat gccatagtca gccatattga
     4201 ttacatctaa aggaatgcct ttaggtgcta gcttttcagc aaattgattt tgcataaagc
     4261 gtacttgcgg tcctaaaagt aaaacgtcta catttttaga ttctagttga ttatccgcat
     4321 ctgaagcaga tacagcaaag atgtctgctt ccattccttt tgcttctgct gccttttgca
     4381 tttttgtgac taggagactt gtactcattc cagctgaaca tacaagcatg attgttttct
     4441 tttccatttt aaacacgttc ctttctatac ttacacttgt atataagcaa gtagcgtgcc
     4501 aactttaaaa gcgctttaaa aagttgatat catcacattt aaggtctata ccaagttgca
     4561 tacacactaa aaaagaaagc gcacactaaa cacacaaatt aatttttatt accttttttc
     4621 ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc acaacttttt tcaaataaac
     4681 ggt
//
LOCUS       DBNIBO010000162         4517 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.169, whole genome shotgun sequence.
ACCESSION   DBNIBO010000162 DBNIBO010000000
VERSION     DBNIBO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.169"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(3..2060)
                     /locus_tag="AC1Y77_002447"
     CDS             complement(3..2060)
                     /locus_tag="AC1Y77_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIQ1575986.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2174..2386)
                     /locus_tag="AC1Y77_002448"
     CDS             complement(2174..2386)
                     /locus_tag="AC1Y77_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575987.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            2573..3040
                     /locus_tag="AC1Y77_002449"
     CDS             2573..3040
                     /locus_tag="AC1Y77_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575988.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            3037..3279
                     /locus_tag="AC1Y77_002450"
     CDS             3037..3279
                     /locus_tag="AC1Y77_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HIQ1575989.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            3297..3599
                     /locus_tag="AC1Y77_002451"
     CDS             3297..3599
                     /locus_tag="AC1Y77_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HIQ1575990.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            3671..>4517
                     /locus_tag="AC1Y77_002452"
     CDS             3671..>4517
                     /locus_tag="AC1Y77_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1575991.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSK"
BASE COUNT         1457 a          704 c          871 g         1485 t
ORIGIN      
        1 ttttatttga agattggatt cgcactaata aatacattat ctacgccaat tctaccagca
       61 gagtcaacta caaaattatg acctaatccg agaatacttt ggttattata aagtaaattt
      121 gcattatggg cagggctatt tttccatcct accatggcat cgatagcacc atcgtctgat
      181 aaaaggtcat caatatacag cccagtttga cttatgtttt ctcccttagc accgtagcag
      241 cctaagcttt ccaataaatc ttggaaagta ccaccaccag ggcgatagtg actgaagctg
      301 acttgtaatt ctttagctcg aatatctgca gcctgttgaa caatatcatt gaattgcatt
      361 ggggcaacac cattttgttg gcgataatca tttaaccaag caagttcttt ttgacgcata
      421 aagttacgta attcgtctaa tgaaatcttt ttatatttga atgtgattgt tttattttga
      481 tcgacgttat taatggatcg ttggccgttt ttatccacgg taacggtagg atcgtcattt
      541 ctgcacaaga aataattttg atcagtcatg tttttccaag gagtagcaaa gctatcccct
      601 tcgtatactg tttttgattc actagagaga acagcgttgg tatcagcatt tacatgatta
      661 acagtcacat taaatttacg agtgtagttg aaaatataat tttgactttc agtcacattg
      721 tttaaggtta ctgaatttgt tggttctacc cgatcgtcta aaacatatcc ttgaatagaa
      781 ggagcagtta aagtgacact gtcaccgtga gtgacattga ctatctgttc tttcaaaata
      841 ttaactcgtc cttcgttttt gtagttgtta ttgcacaaca aattgatttg atgcacttga
      901 ttataattga atgtgattgt tgtattacct gtgattgttt tgctttgagt atcattacca
      961 atcactttgt agctaaaatt atctttagcg ccttcgctat atttaaatgc ttttgcctta
     1021 gcagtgtaag tagaaccaga agttactttt gcttgatctg tagcaatcac ttgacctgtt
     1081 tgattgtcaa tatggttaat agtaaccgta tattcttttg gtgtttcagg tttgtgttgc
     1141 aaccctttaa atgcagcatc aagactattt ttagcattgt ttacttgtgt ttgagaaacc
     1201 ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat
     1261 tcttctgtaa aatctccttt agcagttgat tttactcggt tgtataaagc ttgtaattca
     1321 gctttattca cagtaggaac tggttttttc tttaatcctt tgtaagcaga atctaattta
     1381 tttttggcac tatccacttg cttttgagtc gcttttgagt catttaacac tttcttagca
     1441 ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttcgtt
     1501 cccttaacgc tcgtataaag agcttctaga gccgatttat tcactttggg tttagaggtg
     1561 tcagcgccat accaaccgat accttcgtat ttccagccat agtctttggt taaccattgt
     1621 acttctttcc aacttgcagt gtagtgatgt gagccagatt ttacaccagg aaggaaaagg
     1681 cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccag
     1741 ccgtaatttt tagtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga
     1801 agacctttat tgtacaaacg atagacaaga tggccaccgc tagtcggtgc ttcccaggcg
     1861 gggccttcat aatttcccca ttttatcttt actagatggt cacgttcttt tttgctagag
     1921 gtgtaaaaat gttcattatt atttggattg taaaggcgat acatgacgtt agttgaagca
     1981 gcgtcaacag atccagcaag actaagtgag gcagcaaatg ttccaatagc taaaacagtt
     2041 tttggattaa ataatttcat tttttcaact cctaaattta attaaaaatg tttacatgat
     2101 tattatatat cggctaaccg atatagtcaa taaaaaataa gaaaagcctt atataatttt
     2161 ggcttttctt attttatttg ttagttgaag tattaatatc aacgatttct tcagccaatt
     2221 tatttaattt catagcattt atgaatctca tgttgtctat actagagcgt ttgttggcaa
     2281 ggtcacttac tgtagttaag ggtacgccag attctatgga tatagaacga actgatttac
     2341 cactatcaaa cagcaaccat tcgattttct ttttatcagc gtacatttca atgctccttc
     2401 ttgttttttc tttattatag ctcttttttg taatgaatca caatatcaag agaaaaattt
     2461 tctatagaaa ggtggatttt tatgagtatt cgttctccgt gtatccaaat gaggtttgtt
     2521 gctactttcg ttatttgata tactaaaaat aaacaacgaa agaaggaata aaatgaagaa
     2581 aaagcaacta tggtttgtct ctatgtttgc aatatttttt attattttaa gtggatgttc
     2641 aagtaataag aaatcggaag ctaaagaaga taatagttta ttgcaaccag gaactgtttg
     2701 gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt atagatgata ctacctggga
     2761 atattctgaa agtgtatggc atccagatcc tgtacaaata acagttaaga ggcaaaaaga
     2821 ctatgaaggt ttagaaagat acaaaatagt agattctgca ggcattaggg aatttataaa
     2881 caaacctgat tcattattta ttgttgttcc ctatgagaag gatggcgtaa aaaaaataat
     2941 ttttcttgga tctagcaagg atgagaagcg aacgaaagag aaattaattc atgatggatc
     3001 agagttaaat aaatacaaat tacaaaagac tagtgagtga ttgctagtct ttttatttat
     3061 cctgaaggag gaatcacagt ggcaaaagtt aaaacaccat tcgaaaaagc cgcgtctaaa
     3121 atcttagaac gccgtggtgt ttctgaaata cagtggaaaa aagaagttct tgaaaaagca
     3181 caatttgacc tattacgagg aaaagataag gacttagaag aatatatcta tcaacgtgaa
     3241 cgtgaaaaac ttgtattcga tgaaattatt aaacattaaa aggagcgaat aatcttatgt
     3301 ttgaatcaat tacaatgaat tttttaactc aaccgaaagt agtagctgca gttgatattt
     3361 ttagagcatt acaaactgcc gcaactagtt tgaaaaataa tctgatgatt tttgctgctg
     3421 ttgcggcagt ggcttccatt gctcttggag gtattttctt cttctttggg cgaagaggag
     3481 ctgaaaacgg gaagacacac gtaggttatg tattgttggg ggtaggagtt attttagctg
     3541 ccgcttctat tgtagtttca atgttctcaa tgtttggaca aaccccacaa ggactataaa
     3601 aaaaacaagt aggaggagta aaaagtacgt ggctactttt tactcctttt tttatgaaag
     3661 gagaatattc atgtttttta cgatttcagg aaatgcaaaa ttggataaag agttaataag
     3721 aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa ttaaatattc
     3781 tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac aagagaatca
     3841 atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag aaaaggctgg
     3901 cacagaatac gtttttggcc cactggatta tcaaatttca gaaggttttt atcctacatt
     3961 gatagattta atccgccttg atgtgtttaa agatacggat gaaactatga gtgtggaaga
     4021 gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga gcgaccaaag
     4081 aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa gtaccatggg aacgaccaat
     4141 ctttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac aagctgatat
     4201 tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat tggaggtaga
     4261 tccctttgaa gaaacaatgg aggatgaaga gacggacgaa gtagaagttg atccagttga
     4321 agctaatgaa gagtcaacat ggactgaagg ggattttcag gaatatattg aaccacttat
     4381 cagtgaagaa gtagaaaaaa acgaaacaga gatacaaaag gcaactgtat ttgattatat
     4441 tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaacaaat tatttttaaa
     4501 tgagctttat tcgaaat
//
LOCUS       DBNIBO010000163         4494 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.170, whole genome shotgun sequence.
ACCESSION   DBNIBO010000163 DBNIBO010000000
VERSION     DBNIBO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.170"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(213..443)
                     /locus_tag="AC1Y77_002453"
     CDS             complement(213..443)
                     /locus_tag="AC1Y77_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HIQ1575992.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="AC1Y77_002454"
     CDS             complement(673..1491)
                     /locus_tag="AC1Y77_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIQ1575993.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="AC1Y77_002455"
     CDS             1652..2341
                     /locus_tag="AC1Y77_002455"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIQ1575994.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="AC1Y77_002456"
     CDS             2355..3857
                     /locus_tag="AC1Y77_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIQ1575995.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="AC1Y77_002457"
     CDS             3870..4346
                     /locus_tag="AC1Y77_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIQ1575996.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1502 a          647 c          792 g         1553 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttattatcca
     1621 aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatt
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttggg ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaag
//
LOCUS       DBNIBO010000164         4476 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.171, whole genome shotgun sequence.
ACCESSION   DBNIBO010000164 DBNIBO010000000
VERSION     DBNIBO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.171"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            325..1053
                     /locus_tag="AC1Y77_002458"
     CDS             325..1053
                     /locus_tag="AC1Y77_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HIQ1575997.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1198..3225
                     /locus_tag="AC1Y77_002459"
     CDS             1198..3225
                     /locus_tag="AC1Y77_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like domain-containing protein"
                     /protein_id="HIQ1575998.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3333..4430
                     /locus_tag="AC1Y77_002460"
     CDS             3333..4430
                     /locus_tag="AC1Y77_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HIQ1575999.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1573 a          717 c          925 g         1261 t
ORIGIN      
        1 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
       61 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
      121 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
      181 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
      241 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
      301 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
      361 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
      421 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
      481 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
      541 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
      601 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
      661 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
      721 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
      781 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
      841 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
      901 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
      961 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
     1021 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
     1081 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
     1141 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
     1201 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
     1261 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
     1321 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
     1381 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
     1441 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
     1501 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
     1561 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
     1621 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
     1681 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
     1741 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
     1801 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
     1861 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
     1921 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
     1981 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
     2041 caaagaagtc ttacttatga tttggaaggt gtcggtcact cttctcggat tatacctgat
     2101 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
     2161 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
     2221 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
     2281 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
     2341 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
     2401 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
     2461 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
     2521 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
     2581 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
     2641 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
     2701 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
     2761 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
     2821 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
     2881 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
     2941 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
     3001 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
     3061 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
     3121 acagctcatc gtggaatcag aatagatatt cctgtagaaa accaaagaat cggaaccttt
     3181 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
     3241 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
     3301 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
     3361 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
     3421 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
     3481 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
     3541 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
     3601 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
     3661 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
     3721 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
     3781 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
     3841 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
     3901 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
     3961 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
     4021 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
     4081 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
     4141 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
     4201 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
     4261 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
     4321 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
     4381 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
     4441 acaaatagta aatttttatt attgtaggat ggttgg
//
LOCUS       DBNIBO010000165         4466 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.172, whole genome shotgun sequence.
ACCESSION   DBNIBO010000165 DBNIBO010000000
VERSION     DBNIBO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.172"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            162..392
                     /locus_tag="AC1Y77_002461"
     CDS             162..392
                     /locus_tag="AC1Y77_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HIQ1576000.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="AC1Y77_002462"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="AC1Y77_002462"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIQ1576001.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="AC1Y77_002463"
     CDS             1803..1979
                     /locus_tag="AC1Y77_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIQ1576002.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="AC1Y77_002464"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="AC1Y77_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIQ1576003.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="AC1Y77_002465"
     CDS             2594..3061
                     /locus_tag="AC1Y77_002465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1576004.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="AC1Y77_002466"
     CDS             3146..4030
                     /locus_tag="AC1Y77_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIQ1576005.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4144..4380
                     /locus_tag="AC1Y77_002467"
     CDS             4144..4380
                     /locus_tag="AC1Y77_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HIQ1576006.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1525 a          678 c          970 g         1293 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcatattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa accttt
//
LOCUS       DBNIBO010000166         4424 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.173, whole genome shotgun sequence.
ACCESSION   DBNIBO010000166 DBNIBO010000000
VERSION     DBNIBO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.173"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            690..1658
                     /locus_tag="AC1Y77_002468"
     CDS             690..1658
                     /locus_tag="AC1Y77_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIQ1576007.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="AC1Y77_002469"
     CDS             1726..2484
                     /locus_tag="AC1Y77_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HIQ1576008.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="AC1Y77_002470"
     CDS             2552..3262
                     /locus_tag="AC1Y77_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIQ1576009.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="AC1Y77_002471"
     CDS             3298..>4424
                     /locus_tag="AC1Y77_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIQ1576010.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DBNIBO010000167         4416 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.174, whole genome shotgun sequence.
ACCESSION   DBNIBO010000167 DBNIBO010000000
VERSION     DBNIBO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.174"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            59..913
                     /locus_tag="AC1Y77_002472"
     CDS             59..913
                     /locus_tag="AC1Y77_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIQ1576011.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="AC1Y77_002473"
     CDS             1010..3259
                     /locus_tag="AC1Y77_002473"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIQ1576012.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="AC1Y77_002474"
     CDS             3262..>4416
                     /locus_tag="AC1Y77_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIQ1576013.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINDVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1557 a          626 c          805 g         1428 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga taccggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atgatgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DBNIBO010000168         4381 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.175, whole genome shotgun sequence.
ACCESSION   DBNIBO010000168 DBNIBO010000000
VERSION     DBNIBO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.175"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            69..992
                     /locus_tag="AC1Y77_002475"
     CDS             69..992
                     /locus_tag="AC1Y77_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HIQ1576014.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1008..1547
                     /locus_tag="AC1Y77_002476"
     CDS             1008..1547
                     /locus_tag="AC1Y77_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HIQ1576015.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            2232..2405
                     /locus_tag="AC1Y77_002477"
     CDS             2232..2405
                     /locus_tag="AC1Y77_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576016.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYN
                     SPNEELFVKETDK"
     gene            2811..3245
                     /gene="bacA"
                     /locus_tag="AC1Y77_002478"
     CDS             2811..3245
                     /gene="bacA"
                     /locus_tag="AC1Y77_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HIQ1576017.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1657 a          654 c          739 g         1331 t
ORIGIN      
        1 gacattattg agtattttta taaagtatgt tacttttatt tcatacttta taaaagtagg
       61 agaagcttat ggcaaatgaa attgtaaaat atgaaaatag attaaatcat attcctctga
      121 gaaaatttaa tagtcgtgaa atgaacttat ttttttcaat tgcttcacgt gtacgtgaaa
      181 aaggtgtaga tgagattatt ttttcatttc acgatttgaa aaaactaagc ggttatacag
      241 aacgtgggga acaatttata aaagatctta gtagcacgta tgacaaatta ttaagtatta
      301 atgcttggaa tgatgatgga aatactttaa ctaaatttgt tgctttcaca aaatatagca
      361 ttatacgaga taaacaactt gttataattt cagttaatcc agaatttaaa ggacttttta
      421 atcaattaaa gaactggaca agatttaatc tagagcaatt tactaatttg gatagcacat
      481 attcaaaaac tatgtttcga ttattaaaac aatatcgaac tcaaggctgg actgaatttt
      541 ccaaagaaaa attttttgat ttactagata ttcctaaaag ttattggaat agtccgtcaa
      601 atattgacaa atttatctta aggccaatta aagaagaact cactccctta tttaaaggat
      661 taactattcg taaaaaatat ggtaaaggtc gaggtaaacc tgttatcggt tatcgtttta
      721 cttggaaagc tgaaattaac catgctgacg atttttccaa agggaaacaa gaagatttac
      781 gtataaaatt atttaatatt gatcacaatg gagaattaac acgagaagaa aaatggcgcg
      841 ctaaagatcg aatactaaat cttcccttag gcactcatga agctgatttt aaacaacaac
      901 aacaaactga agaaaaacag gcagaaaaac gagaactttc aaacaaactc aaacaagatt
      961 tactaggaaa tttacaaaac ttattcgatt aagcttaagg agtatttatg gcagataaaa
     1021 caatcaaaga acttgcggaa gaattaggag ttagtaaaac agcaattaac aaaaaagttt
     1081 ctgacaaaaa tcgaaaactt tggtttgcaa aaaacggaaa caagtttgtg attaacgaaa
     1141 ccggtcaaaa agctataaaa tcaatgtttt tagtcgataa cgcaaactta aatcgaaaac
     1201 tagttggcga aaaatcaaaa accaaaaacc ataaaaacac caaccaagtt tccgaattaa
     1261 aactcctttc tttcctagaa aaacagttaa atcgaaaaga tatacagcta caagaaaaag
     1321 ataatcaaat aaaacaaatg caaaaattac tcgaccagca acaacaactc actctacaag
     1381 cgaaccaaca aattgagaac ctacaagaac agctccattt aacttataca gaagaaactc
     1441 caacaaccga atcaactgcc ttttctgaaa aaaaagcaga caatacacaa ccacttattg
     1501 aaaaaaagtg gtggcaaatt tggaaatcta atagaaccaa tctctaaaat tttctagaag
     1561 ttagttcgga tagtagagga aaaagttgta ctacctgatg gggagatcac tcaaattttt
     1621 atgagaaaga aaaaatagtt tacaactgta aacaatcaat gtttatagtt gtaaactatc
     1681 aaactatatg gtgacttatt gcttaaaata ggttgaaatc tcactcaatt tttggtggaa
     1741 aaacaaaata aattctaatc gattttgttg aaaaagtcat aaaaaaacaa atgtgatttt
     1801 aacaaaattt caaaaaaatc gtttgaaatt tcgtttattt tttttgagaa ttttgatcat
     1861 cgtagggtca ttgtagaatc attatgagat catcgtggga tcatcgaata aagtatggga
     1921 aaggcttgcg gtgcttggta ttaagggcat tctccgcctc ccaggttctt gaccattttc
     1981 tctcaacgtg gtaaaaatcc cacttataag agaaaatggt caagaaccta aacggtcggc
     2041 gaagaaaaca taccaagcac cacaagcaaa aaaagttaga gtaacttcca aaaaagaaaa
     2101 attttttaga acaacttttg aaaatttaat tttgatttat atccgttcaa tttaaacatt
     2161 tgataaactg aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg
     2221 aggtataacc tatgttaaaa aaaactgtgt tggtactggg ttctatttct ctaggaatat
     2281 taggttttag ccccatgcta ttatccgtaa ttggaaaaaa caattccaac ggagaactta
     2341 aattaaattc aaattattac aacagtccta acgaagaatt gtttgtaaaa gagacggata
     2401 aataataaaa aagactgctc tttcgagcag ccagttaaaa aaataatgag agtttattct
     2461 tgaaaaagca acgcgcttat aaagcgctac caaaatttta tcatatttta taaaaaataa
     2521 caatccagta aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caactacaga
     2581 aaatcaagcc ttaaaaagag ctttttacct agaccaatta aaaatcacaa agtgagcgtc
     2641 tctatccatt ggtagagggg ctacttggca tagtgtacat tatgtctact aacctttaaa
     2701 ataacacgtt ttataaaata aaccctcttg acaatattta attttctgat tattctaaat
     2761 atatacagaa agcgctttcc taggatttta tatctaagga ggaagtaaca atgaaaaata
     2821 aaattattaa aattattact tgtctatttt tgttcggtat gattgttgga gcaagtactc
     2881 cgttaaatct gtttcctttt agtgctagag gtataaaagt ggaagctgca agttcacgat
     2941 ataatcataa tcatcgtgga tttacttgct ataaaatgaa ctattatgtt actaataaaa
     3001 tgtgtagaga tttgaaaaaa aattataata agctaaagac tcctgctcaa attgcatctt
     3061 ttattcctat tggtggagtt ggaacatggg ttattacaaa tgcttttagt agtgctgttg
     3121 atgcaatgaa tgtctttgta agagctgcaa atcaaggaaa aggtgtacaa ttaacatata
     3181 atgctcactt tagcaataca acatcatatc aatacaatga ttacgctcgt tatgtaataa
     3241 aataatcaga aagtgaaact atctcatgaa taaaaagatt ttagctgtaa ttatttcaat
     3301 aatagtttta atcactatgt tcttcatatt taggctaatt ttcaatataa gcttacaaaa
     3361 aagtattctt tatctaattc ctatagcatt tatcttagca atttttagaa gtatttacgg
     3421 aaataaaaac tctaaatagc taaaaataaa tcatactaaa ttttacaaaa agtggctagc
     3481 attttctagc cactttttgt attgtggagc gtgggttttg agggggattc aagggggagc
     3541 ttgcgcccct ttgacaagcg gttatttgca ccaaatacgg aggtatttgg ctagcaaata
     3601 tagagcttgc caaacccttt tagtgagttt tgtagaaagt acctttaaag gtactttcac
     3661 attacgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag caaattaatg
     3721 caaaatttgg ctagcaaaaa agctaaaaaa tatatgctaa taatcgagcg aaataaacac
     3781 tttttcaagt gagcgaatcc gccatgacaa agctgaagca gtttatttca gctttgtcat
     3841 ggcgtgtgcc gactttttta aaagtctagg cacacgagca aatatcttta tcaagctcta
     3901 atttgcgttt taagacgttt gaaacgccta agtatataaa tatataagaa taggtttaag
     3961 agtgcttaaa tcgaatttta gagggcattt agacaatttt agggttgtta gggcttgccc
     4021 tgaccgcctg taaggcgttt taagaacaag ataagggatt tatactagga ctgtcttaaa
     4081 atgtcttaaa cgaacgctca gagcttctca aagggttcct tatgaacggc ttttagcaaa
     4141 ggagccggag gcgactgcgc agtataatgt gagagcagag cgatcacacc tttttagcga
     4201 accgtaggga gcaaggtttg gaattggcag ttttgataaa ttcgcgaacc tgtttagaga
     4261 aaaaaggagt gattttttag gcgggaaaat tagcctcccc tctttttata tctcttttta
     4321 tatctctttt tatatctctt ttatgtcggc tgtgtcctta ctctcccaag caattcagag
     4381 t
//
LOCUS       DBNIBO010000169         4336 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.176, whole genome shotgun sequence.
ACCESSION   DBNIBO010000169 DBNIBO010000000
VERSION     DBNIBO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.176"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..567)
                     /gene="sgrA"
                     /locus_tag="AC1Y77_002479"
     CDS             complement(<1..567)
                     /gene="sgrA"
                     /locus_tag="AC1Y77_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HIQ1576018.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTES"
     gene            complement(763..1602)
                     /locus_tag="AC1Y77_002480"
     CDS             complement(763..1602)
                     /locus_tag="AC1Y77_002480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIQ1576019.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1787..2674
                     /locus_tag="AC1Y77_002481"
     CDS             1787..2674
                     /locus_tag="AC1Y77_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIQ1576020.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3258..3953)
                     /locus_tag="AC1Y77_002482"
     CDS             complement(3258..3953)
                     /locus_tag="AC1Y77_002482"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIQ1576021.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3937..4335)
                     /locus_tag="AC1Y77_002483"
     CDS             complement(3937..4335)
                     /locus_tag="AC1Y77_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HIQ1576022.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1427 a          864 c          645 g         1400 t
ORIGIN      
        1 actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc
       61 tgtactgctt tcaccgctcg tgttttctgt actgctttca ctacttgtgc tatccgtact
      121 gcttggcggt ccaacaactg tccaactgct tggtttcaaa ttctccgtga tcgtcatctg
      181 tccatcataa aaaacaatca aatcaacttt atctttactc ttgatctcat caatcgatag
      241 ttctgcagaa atcattattg tatactcaat accagcggtc cattgtacgt cttttaaata
      301 aacttcccca ataacttttt ctgaacttcc attaccatcc caaccatatg ttagaacagc
      361 atgtacatca ctagcattca cgcttttgtt tggtttgact ggaatagtaa tgacttgttg
      421 tgtcatgtca taaacaatcc gatcagtttt cacagtaaat gaaagattct catttgcccg
      481 ttcatttgca gaagtactga ttccaatcat tgctgcaatt atactcagcc caagaataac
      541 aaataatacg gtcgctgttt tcttcatttc tatcactctc catataatta ccttctattt
      601 gataaaggaa ttatataaaa tgaaaatgat gagaacacga cagtcagttg tgaaaatcta
      661 acaaaagatg tcgtgtattc gcttgaattt gtttactttt tcgtttaatc aatgacagac
      721 ttaaaaaagc aagaccgata tcagccttgc tttcttcatc ttttattgat aaacagcagt
      781 gtatttcttc atccgctttg ttttatatgc atagagtaac aaaccaacaa tcaagactaa
      841 caagaataat gtagttgata tagatgcttc agccccaaag tttcccccag taattattgt
      901 attttgcgat tggatcagag tggagaaaaa accttgttct tgcgttccgc tgactggtat
      961 acccaaaata gctccttgtg tccaattcca agtaatatga gcaccaatag ccgtaaataa
     1021 acttcctgta ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac
     1081 tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc
     1141 aatcattgct attaacggac caaatctaag catctgatgc tgaaaaaagc cacggaataa
     1201 aagctcttct gatgccccca ctgcaataaa taaaataaaa ctcgttatca gcggcacaat
     1261 gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa tataatggac tagtaataag
     1321 cagaaacact ccaattccaa caacgagacc tcccgctaaa tacccccagt cttttcctcg
     1381 aacagaaaca ccataatctt ctattctttt tccttcgacc ttactagcaa gaaataaagc
     1441 tagtaaaaaa acagctaaat gtccgaccgt accggcaatt gctccaatcc attcactatt
     1501 tccaataaac atatccgcaa gtgcattatt gacaagaatt cctaaaacaa aaccaactaa
     1561 gactattaaa acagatccaa tcaaccgaat aatcctgatc atctttcatc cctcctttat
     1621 attaagttta acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc
     1681 atagagagaa tagattttta cgaactaaga atatctacag caaccttacg aaaatgaaag
     1741 actgtctccc taattcttct gttagactgt ttttaataag gaaaggatgg gaaatttttt
     1801 gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca attttactat tctcaggttg
     1861 tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga acacttacag ttcaggattt
     1921 ttatgataac ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt
     1981 caaagcattt gatgatgtat acggaaaaga gatttcttca aaggaagtaa caaaagaata
     2041 taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag cagttggcca
     2101 aacagaagag acatataaga aactctttaa acagatgctg gctttccaat acggtttgaa
     2161 agccaatgta aaattaacag ataaagatct agacactgct tggaaagaat tctatccaga
     2221 agttagtaca cagatcattc tattttcgac taaagaagaa gcagataaag caaaaaaaga
     2281 agcaaatgaa ggggaaaatt tctccaagct tgttcaagct tatggaaaaa ataaaacatt
     2341 aaaggaaact gatggaaaaa tgaattttga ttcaaccaat ccagagattc ctactgaagt
     2401 aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct gatatcattc ctgtaacaga
     2461 tccaacgact tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac aggataaagg
     2521 ttctaataaa gacaaataca aatctgagct agaaaaaatt gctaccgaag caaaaactct
     2581 tgatacggaa ttcatggata aaacaatccg taaagtgatg aaaaaagaca atgtaactat
     2641 taaagatcct tatgtaaaaa atatattcaa ataaacaaat ttgacccaat tggggatgtg
     2701 gcaaaatttg agaagtgatt tttggaaatt ccttcttatt tttgaggcaa accacttttg
     2761 ccacaacttc atcggggcct ttttttattt gtaccaaaac aaatcactgg atttgttaca
     2821 atcatgagga tataatttaa aaaagttttt tatttctatc acatgaatca atgcgccatt
     2881 aagtagattc tttcatgctt ccctattttt tcacttgccg tatcgcttca aaaacaacta
     2941 tcataaactc aaactcgact gaaaagttat tttaaaaaag actaaaaata tcccttctgg
     3001 acttgttcct cttattctgt tatactagag aaacacgaaa gatattttta tgcgacactt
     3061 ttcatttgtt tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta caaggatgta
     3121 ttagaggtta gggaatacat cgtttaaatg agaaataagt aatagtcacc caaactttaa
     3181 attaatgaat attgaatcgt cagatagcct cctcgagttt tcgagaaggc tatcgtttat
     3241 tttatggaga aattgattca gaagtaatca gcgacctttt ttagttcacc aatcgtttct
     3301 gcttggaaag tttgcaccca atcttcttgg aagcccctca ctgctttatc aatcgagggc
     3361 atctctaata cttgatctag tagctcttta cctaacgtta tcgcatgagc tccagatcga
     3421 atggctgtag tgacttgttc acaatttttg aaactagctg ctaatatttt tgttggtctt
     3481 tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc aatgccttga
     3541 ttttgcatcc tgttataata aggtgcaata taatctgctc cattattaat agctaaatac
     3601 ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt tttcaacttt
     3661 ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga taaacgtatc ttttcccagt
     3721 tcttctatga taactcgagc atcctctatc atttcttctt ccgttcttcc tattgtctga
     3781 atatggatag gaatctgtcc gattattttt ttgatttctt tcatatgcga gaagaagttt
     3841 gacgctcctt cttttttcag aattgtagga tttgttgtta cgcctgttaa attcaaaaac
     3901 tgttgacaag aataaatact ttttgtattt gctgtatcaa gcatgatttc catatagctt
     3961 tcccccattc tgatcctctt gatcgatttt aatgattgtc ccctttctta aatgcggcat
     4021 ttccttatct tctacataca ttgttcctgg aagttccggc tcatgagatc catcaaattt
     4081 tatcgtgata tgtcctaatt cattcaaatt agtttggaca acatctccta ctgctgtgat
     4141 tacatacatt tgtttatcaa aacagagctt catatcacaa gttatttctt ttcttacctg
     4201 attcaattca atcttataac aataatctgt taatgtttcc ggtgcttcat ctccaaataa
     4261 aatcattatt ttttctttcc tgaatacttc tgcttctata ccaacatctg ttacaaccgt
     4321 ttcaaagatc gtcatc
//
LOCUS       DBNIBO010000170         4299 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.177, whole genome shotgun sequence.
ACCESSION   DBNIBO010000170 DBNIBO010000000
VERSION     DBNIBO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.177"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..156
                     /locus_tag="AC1Y77_002484"
     CDS             <1..156
                     /locus_tag="AC1Y77_002484"
                     /inference="COORDINATES: protein motif:HMM:NF012267.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIQ1576023.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="AC1Y77_002485"
     CDS             175..1452
                     /locus_tag="AC1Y77_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576024.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="AC1Y77_002486"
     CDS             1462..2118
                     /locus_tag="AC1Y77_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1576025.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="AC1Y77_002487"
     CDS             2118..3494
                     /locus_tag="AC1Y77_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576026.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..4037
                     /locus_tag="AC1Y77_002488"
     CDS             3624..4037
                     /locus_tag="AC1Y77_002488"
                     /inference="COORDINATES: protein motif:HMM:NF013068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1576027.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4062..4299)
                     /locus_tag="AC1Y77_002489"
                     /pseudo
     CDS             complement(4062..4299)
                     /locus_tag="AC1Y77_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1681 a          506 c          750 g         1362 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     4141 cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa tattcgttca
     4201 atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg aagatgttcc
     4261 acttcatcca tcagatctcc ccaaacatgt ctcgtgatg
//
LOCUS       DBNIBO010000171         4268 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.178, whole genome shotgun sequence.
ACCESSION   DBNIBO010000171 DBNIBO010000000
VERSION     DBNIBO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.178"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            58..510
                     /locus_tag="AC1Y77_002490"
     CDS             58..510
                     /locus_tag="AC1Y77_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576028.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="AC1Y77_002491"
     CDS             524..1213
                     /locus_tag="AC1Y77_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576029.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="AC1Y77_002492"
     CDS             1338..3689
                     /locus_tag="AC1Y77_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HIQ1576030.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3778..4164
                     /locus_tag="AC1Y77_002493"
     CDS             3778..4164
                     /locus_tag="AC1Y77_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576031.1"
                     /translation="MEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYF
                     ANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEY
                     NYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1508 a          535 c          861 g         1364 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttattta
//
LOCUS       DBNIBO010000172         4259 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.179, whole genome shotgun sequence.
ACCESSION   DBNIBO010000172 DBNIBO010000000
VERSION     DBNIBO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.179"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(78..2525)
                     /locus_tag="AC1Y77_002494"
     CDS             complement(78..2525)
                     /locus_tag="AC1Y77_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIQ1576032.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(2714..4150)
                     /locus_tag="AC1Y77_002495"
     CDS             complement(2714..4150)
                     /locus_tag="AC1Y77_002495"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIQ1576033.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         1084 a          990 c          729 g         1456 t
ORIGIN      
        1 accatttttt cttttggtat catcatgcct tgtcttttta ttggttattc gtttttcaac
       61 ccttcatcac acaagtttta catgtcatta tcgtccggca acgtttctga tgtttcttcc
      121 tgctcaatga atactttccg aggcttgctt ccttctgacg ggccaacaac tccatgagct
      181 tccagttcat ctactaaacg tgctgctcgg ttgtaaccga tccgaaatct tctttggaga
      241 agggaaatac ttgccgtttg catttcaacg acaagtgctt ttgcttcttc aaacagctca
      301 tcttgtggct gttctggagc acctgtcgtt tcaacttcat ctgtcggcat cattttctct
      361 tcataatgag cttcttgctg atctgtaaca aattgaacga cccgttctac ttcgtggtca
      421 gaaatgaatg ctccttgcac acggatcggt ttattctcac ccattggcaa gaatagcata
      481 tctcctcgcc ctaagagctt ttctgcccca ttagaatcaa tgatcgtccg agaatctgta
      541 ccgcttgata ccgcaaatgc cattctggaa ggtacatttg ctttgatgat tcctgtgata
      601 acatccacac ttggacgttg agtagctagg atcatatgaa tccccgctgc ccgagccatt
      661 tgcgctaaac gaataatcgc atcttcaact tcattgctgg ctaccatcat caaatcagct
      721 aattcatcta cgatcactac gatgaatggt aaggttggat gtttcgttcc gttttccaag
      781 tttttctgtt gaacaaattc attgtaacca gaaatatttc ttactccggt tgccgcgaat
      841 ttttcatatc gttcttccat ttcttgtact actttttgta aggcttgcgc tgcttttcta
      901 gggttcgtca cgaccggcgt caacagatga ggtatgccgt tgtacatatt caattcaacc
      961 attttcggat cgatcatcat cagttttact tcatggggct tagcacgcat taatatactt
     1021 gtgataatcc cattaattgc tactgacttc ccactaccag tcgaaccagc cacgagtaag
     1081 tgaggcatct tcgaaagatc agccgtttgt acccgtccag aaacatcacg tcctaaaggt
     1141 acttctagta atttatctgg gtggtttggt tgtgcttcga tgatttctct aaaggaaacc
     1201 atactgatct ttccatttgg tacttcaata ccaatcagtg atttacctgg aatcggtgct
     1261 tccatccgga catctttcgc cgctaaagca agcgcgatat catctgttaa gttcacaatt
     1321 ttacttactt ttacccctac agccggctgg acttcgaatt ttgtgacgga tggtcctaag
     1381 ctggctttga cgaccttggc atcgacaccg aaactttgga aagtttgttc taatacgccg
     1441 atattttttt caattttttt atactcatca ctttgatcca cagttggtat agaatcaagc
     1501 aaatcaactg tcggtaattc atagtcttga ttttcctgtt ctgcttcaat ctcaaattct
     1561 aatgaggtac cgtcatcctc tggcaactca tctgaaaaac cttctgctgt attggtctgt
     1621 tttggtgtat ctggtactgg tttctctaga ttcgcaggat ggatattttc ttgaaaactg
     1681 tcgattggaa caaaacttag ctgctccggc tctgtatttt ctggttcctg ccattcatca
     1741 gcagtatttt ccctttgctg gctcaccgct tttttcttct catattctgc agcttctttt
     1801 tctgcagctt cttttgcagc taagcgcctt gcttctttcg cttctgcgcg ctgtttcatt
     1861 ctctcttcct ttgccgcttg ctttcgagct tgcttggcag gatcaccttc caataattcc
     1921 tgtactcgtt ctccaattga ttggatacca tttaacagct gatagccttc cagattactg
     1981 aacaggaatg ctcctcctgc cagtaatagc actgctgcta aataacttcc aggctgtgca
     2041 atcaagaagt aagtaaaatg ataaaggatt gctccaatca tcccaccacc aacgttattg
     2101 tctacttgac tttgttttat atcactggct aacaaatccc atgtcgtccc catgatattt
     2161 ggttccccag tatggagttt gccaaataaa tgggcatgta acaataataa gataccgaaa
     2221 taaaagagta tgccgccaaa ccagcgtcgg tttttaccaa ttgaaaactc tgtattctta
     2281 atgacaatcc aaaatcctaa tacggctaat aaaagacaga ggatttggta agtatttcct
     2341 gcaatgatcc gaaagccgtt tgcaatcaat attcctaaaa aacctaattt taaaaagccg
     2401 aagacagcaa ataaaatcaa ggcaaaccca acaccaatcg tcagcatttg ttcctgctgc
     2461 tgtttttgct ttttcgtttt tttcgtcttt ttcctttttt tagtaggccg tttcttttgt
     2521 gccatttatt atccctcatt tcatcaagtt tcattataca aaaaaaagta agggaactaa
     2581 agttttgttt gaaagaactg acgctttctt tcatttttca gcttatttta cagtttttct
     2641 taatttctaa atgattttat caagaaaagg cacggccata tccagccgta ccttcttgtt
     2701 ttaagtatgt gaactaaagg ttctctccat tttcttcaat cagtttttgg taccagtaat
     2761 aactgtcttt cttatatcgt tccaaagatc cttcttgtgt ttcgtcttga tcaacatata
     2821 caaatccata tcgcttttga taaccgttca gccagctaag caaatctgta taagaccagg
     2881 tgcaatagcc taggacttct gctccatccg aaattgcttc tttgatggct ttgacgtggc
     2941 tttgtaagta attgattcga tagtcgtcat ggatttgttt atcttctgtc aacttgtcat
     3001 actctcctaa gccattttcc gtaatcagta ccggcatgcg gtaacggctt gtgatacgac
     3061 gcagtccgat ccttaatcct tctgggtcaa tctcccagtc ccaatttgtt cgttcgacaa
     3121 atggattctc aactttttta tacattccag gaactccagt ttcttctgat gaaccttttt
     3181 ttcctgtcgt attcattttt ccagcaccaa ctccgtttaa tggattgaat gcattcgtcg
     3241 ccgtttgata gtaattgatc ccaagaaaat ctggtttagc agattctagc aattctgcat
     3301 cgccagcttc aatcgttggt gcccatcctt tttcttctaa gaacctcatg gcagcaatcg
     3361 gatattttcc ccacatatag acatccatcc aataattggc catcaggtct tctgcatttt
     3421 cagcagccag aacattttca ggattactgt caattggata atttggcgta tacgcaaagc
     3481 ttggtccgat ttttccaggc atttctaact cgtgaaattt attgatgaca gatgcatttg
     3541 ctaaattagc aatatggtta acttcataca ttcgtttcgg gtctgtcaca cctggcggat
     3601 gtgctgctaa caaataacct aatgaagtga agatattttg ttcattcaaa ctaatccagt
     3661 aatggacttt cccgcggaat gcctcaaata atgtttctgc ataatgagtg aaatcttcaa
     3721 tgatctgacg tgattcccaa ccattatatt catcttgcaa tgcttgtgga agatcccaat
     3781 gatataaagt caacataggt tcaatatcgt tttcaatcaa ttcgtcgatc aatctctcat
     3841 aaaaagcgag cccagcttga ttgacttctc ctcgtccatt aggaaaaatc cgtgtccaag
     3901 ctacagagaa gcggtaagct ttcagacctt gttctttcat taaagcaata tcttctttat
     3961 aacgatgata gtggtctact gccaaatcac cgttcgttcc tttaaatgtc tttccaggta
     4021 tccgtacaaa tcgatcccag actgattctc ctttcccatc ttcttgccaa gctccttcta
     4081 cttgatacgc tgcagaagca gagccccaca gaaaatcttt tggaaatgag gtaagttctt
     4141 tgtgttccat tcatctcttc ctttcctata accttcaatc gttcctcttt tagaataaca
     4201 cgaaacccct tccacaacaa aataaaaata tgtaaaggag gttgtgaaaa agtcgctta
//
LOCUS       DBNIBO010000173         4224 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.180, whole genome shotgun sequence.
ACCESSION   DBNIBO010000173 DBNIBO010000000
VERSION     DBNIBO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.180"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(77..607)
                     /locus_tag="AC1Y77_002496"
     CDS             complement(77..607)
                     /locus_tag="AC1Y77_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HIQ1576034.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            712..1263
                     /locus_tag="AC1Y77_002497"
     CDS             712..1263
                     /locus_tag="AC1Y77_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1576035.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1416..1889)
                     /locus_tag="AC1Y77_002498"
     CDS             complement(1416..1889)
                     /locus_tag="AC1Y77_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1576036.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2027..3499
                     /locus_tag="AC1Y77_002499"
     CDS             2027..3499
                     /locus_tag="AC1Y77_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIQ1576037.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3496..4206
                     /locus_tag="AC1Y77_002500"
     CDS             3496..4206
                     /locus_tag="AC1Y77_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HIQ1576038.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1323 a          758 c          819 g         1324 t
ORIGIN      
        1 atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa
       61 cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat tttccgctac
      121 ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac
      181 ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt ggataagttt
      241 catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga
      301 tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat
      361 ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata
      421 cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca
      481 ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc
      541 aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt
      601 ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat
      661 tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga
      721 aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat
      781 gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag
      841 atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc
      901 acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg
      961 gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg
     1021 aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag
     1081 cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct
     1141 actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat
     1201 cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa
     1261 taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac
     1321 cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt
     1381 attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa caactgttcg
     1441 tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca
     1501 tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag
     1561 cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc
     1621 attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct
     1681 aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa
     1741 ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt
     1801 ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc
     1861 aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc
     1921 ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact
     1981 aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca
     2041 acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat
     2101 tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat
     2161 gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt
     2221 caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt
     2281 atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa
     2341 ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc
     2401 attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg
     2461 gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat
     2521 cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt
     2581 cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc ctagcattga
     2641 tattttatcc gctattttat caacgattgg attcggtagt ttactttacg gattttctag
     2701 tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat
     2761 tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct tggaacttcg
     2821 tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa caaatatggc
     2881 gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc
     2941 attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt
     3001 gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag ggatgttcat
     3061 tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat
     3121 cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac
     3181 ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg taaataatac
     3241 attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt
     3301 gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag cttataaaga
     3361 tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt
     3421 tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaatgaa
     3481 agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta
     3541 acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc
     3601 ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa
     3661 gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg
     3721 cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa
     3781 aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca
     3841 gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat
     3901 aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc
     3961 aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta
     4021 gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg
     4081 gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa
     4141 gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc
     4201 gaataatatt ttgaacacca tgta
//
LOCUS       DBNIBO010000174         4184 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.181, whole genome shotgun sequence.
ACCESSION   DBNIBO010000174 DBNIBO010000000
VERSION     DBNIBO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.181"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(2..1222)
                     /locus_tag="AC1Y77_002501"
     CDS             complement(2..1222)
                     /locus_tag="AC1Y77_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIQ1576039.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="AC1Y77_002502"
     CDS             complement(1242..1847)
                     /locus_tag="AC1Y77_002502"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIQ1576040.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="AC1Y77_002503"
     CDS             complement(1896..2828)
                     /locus_tag="AC1Y77_002503"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIQ1576041.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3249..4109)
                     /locus_tag="AC1Y77_002504"
     CDS             complement(3249..4109)
                     /locus_tag="AC1Y77_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576042.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1187 a          847 c          688 g         1462 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgtttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgat aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt agttaatcaa tagtatcatt tttttcatat gatttaattc atcaattaat
     3301 tttttgtttt tattaatttt agcaaaagct agtgcatctt gaatcaattc gagcacttct
     3361 gccttcgact tatttttagc ctgtgcgttc ttcgctaatt gggcagcagc tcgtgctaag
     3421 taataagtca aatggttatc cgaacaaata tcgattgcgt aatttagtag tgcatcactt
     3481 gtttccaaat caccgtcttg cgcaaaaaaa acaccacaat gaaagacgat atttaacacc
     3541 cgccagatat cttctgttgt acgaatcgga tacgtataaa ttttttcaaa cactcgattg
     3601 aaataaactt ctgctttttc caactctttt tctctagcat agaccattcc aatacctgta
     3661 tatgccaata aacgaaacaa cgtgttggct gtttctgcca tcaacaactg atcaaaatgg
     3721 aacaacacat ccgtaattgg tgcattcgta aaaatcattt caaatccttt tagatagtag
     3781 taacgtgcaa tcagctcaga ctcgtcaatt accttgggat caatggctgc taaaatctgt
     3841 gtcaattgtt catattcact cgtaatcagt gaaaactctg ctttttctag ctgttcatca
     3901 agttgtgtat tgatcacgtt catcgaagaa aaaagttcaa ctaacggaag attgattcga
     3961 ttacataatt tgattaaaat ttttaacgaa gggacttgac cattgttctc aaatttactt
     4021 aatgttgctt gtgtacaaat tccttcagac acttcttttt gagataagtt ggcagccttt
     4081 cttgccgtaa taaatttttc aatgttcatt attttctctc caatcagacc tcacacgaaa
     4141 atcatttctg tctcgatttt agcatgtttt ctttgggaat gctt
//
LOCUS       DBNIBO010000175         4055 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.182, whole genome shotgun sequence.
ACCESSION   DBNIBO010000175 DBNIBO010000000
VERSION     DBNIBO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.182"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(121..806)
                     /locus_tag="AC1Y77_002505"
                     /pseudo
     CDS             complement(121..806)
                     /locus_tag="AC1Y77_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(942..1136)
                     /locus_tag="AC1Y77_002506"
     CDS             complement(942..1136)
                     /locus_tag="AC1Y77_002506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576043.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1186..1626)
                     /locus_tag="AC1Y77_002507"
     CDS             complement(1186..1626)
                     /locus_tag="AC1Y77_002507"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIQ1576044.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(1630..2475)
                     /locus_tag="AC1Y77_002508"
     CDS             complement(1630..2475)
                     /locus_tag="AC1Y77_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIQ1576045.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2624..4042
                     /gene="pepV"
                     /locus_tag="AC1Y77_002509"
     CDS             2624..4042
                     /gene="pepV"
                     /locus_tag="AC1Y77_002509"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HIQ1576046.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         1218 a          790 c          819 g         1228 t
ORIGIN      
        1 ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga
       61 ggaactattt tgccgtttat ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc
      121 ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta
      181 ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa
      241 gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc
      301 taattctgaa atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc
      361 tagccaatcc caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt
      421 atccaaatca aaaaagcttt tttattttga gcgatcactg cctgatcggt ttgttctagt
      481 attttttcta gtttagcttg atcttgcttg gtcattttcg cactgcattc ttgcaggatc
      541 ggtttttcca acttttcccg cgtataacgg gctaatgaag cagatcggat atcgattttt
      601 gaaacataag tgccggactg tggaatggtt tttacaagct cttgattgcg cagttggatc
      661 agtgcttctc gaatcggtgt acgcccgata gctagttgtt cttctaacac tttatcggag
      721 attttacttc cgggtgctaa ctcggaataa atgatttgtt tacgtataga ttcataagct
      781 tgctgttgca aattattgat aaccatttaa ccttcctttc tatgcagata taatctatta
      841 taacataaat cgcttacaaa tcgtgtgggc ataaaaacac agtctgcaaa aaaagccctg
      901 ataggcatgc ctattagagc ctcgaaaaga aaaatattga atcaatctgg tcgtacctct
      961 tggatcggtt tgacacgacc gccgattctt ttagcaaaag cttcggattc ttttttgtct
     1021 ttgaaaatga gcagattgtc taagtttttt tcccgagtgc gacagccagt tttatcgatg
     1081 taaccgtgca ttactttaac gacatacatg gggatcacct gcttttttct tttcattata
     1141 cgaactactc gacacaatac aagaaaaatg cttagacggt ctttattaag aatttacatc
     1201 atccaaagaa gcgtacgctt ggaataactg ttctaactcc ggattgactt tgctcagctt
     1261 cactaatcgt tgcgaatcag aaagtaaaaa acagtgttta ctactgccag tagtttttaa
     1321 ttttttgtct tcagttccca caatttcgta gctaaaatcc aaccgtgttc ccgtatactt
     1381 ttggatagtt gggtggatgc tcactttttc tccgtaatgg atcatttctt tatagctgca
     1441 atttacttcc agaacgggga tgatgatccc agattcttcg atttttttat agggaaaccc
     1501 tagatgtacc aataaggcta cccgagcctc ttcaaaccag cggatataat tgctgtgatg
     1561 aataatcccc atttgatctg tttcataata aaaaggttct cgcacataac ctgtaaattt
     1621 ctccatgact catgcctcgt tttccattga ttttttacaa aaataattca tccacttcgg
     1681 aagtgcttcg ctgatttgtg tcggcaaaac aatatattgt tcttttgcca gctggtctcc
     1741 aatgaaacta tgaagataaa cagctgctgc aatcgtttgg tcattttgag gaaactgggc
     1801 taaaaatcct gtgatcatcc cagctaaagt gtcacccatg ccgcctgttg ccatagcagc
     1861 agaacctaaa ggattggtgt agaagtgctg gtgaccataa atttttgtct catgactttt
     1921 caaaacgagc acagcaccga tttcctgttg cttctttttg ttttctttat cccgttgttc
     1981 ttgtatcggt agagaactca accgttgcca ctccatttga tgaggtgtaa gtacagtttg
     2041 ttcgggatag ggtagcaaaa gatgattagc aacaagtaaa cttaaagcag agccatcaat
     2101 caccagccat tgttcttttg attggttttc tagtacttgt ttgaaacgtt ccaaactctt
     2161 agaagaagta cccaatccgg gaccaatcaa gagtacatct gcttgcttta ttacggaaag
     2221 aaactggtca gtatccgtcc aatctacaat cattgcttct ggcagatgac tgtgaagagc
     2281 actccgattt ttttcatcag ttactacagt cgtgagaccc gctccactgt gtacggcagc
     2341 taaagcactc ataatgattg ctccgccata ttgttcattg ccgccaatca aaacgatacg
     2401 tccaaatgtt cctttatgtg aaatctttgg acgaggtgtg atgactcttt tcactagttg
     2461 ttcgtttatc agcatcgaaa aacctccttt tactcattat agcgtatatt ttttttagag
     2521 aaaaacagat aagaaaagga attgtcagat tttttgaact atttttttct gtaatttgtg
     2581 gtatcatgaa cacgaagact taaataagtg gaggattaac tttatgacaa ttgattggca
     2641 aaaagaagta gaagcacgta aagaagactt gcttgaagac ttgaaaaatc ttttacgtgt
     2701 caacagcgag cgcgatgatt caaaagtaac accagatgca ccatttggac ctggacctcg
     2761 cgacgcattg aaacatatgt tagcttatgg cgaacgtgat ggatttaaag taaaaaatgt
     2821 cgataactat gcagggcata ttgatcttgg cgaaggagac gaaacgctag ggatatttgg
     2881 acacatggat gtcgttcctg caggagatgg ctgggacaca gatccttatg aaccagtaat
     2941 caaggatgga aagatctttg cacgcggatc aagcgatgac aaaggtccaa gtatggcagc
     3001 ttactatgcg atgaagatca ttaaagaact tgacttgaaa ctatcaaaaa aagttcgctt
     3061 cgtagtcggc agcgatgaag aaagcggctg gggcgatatg gcttattatt tcgaacatga
     3121 agaagaacca gattttggtt tctcaccaga tgctgaattc ccaatcatca atggtgaaaa
     3181 aggaaatgtt tctttagctt tacgcttcaa aggcgacaat gcaggagatt atgtcttgaa
     3241 atcttttgtt tccggacttc gcgaaaatat ggtgccagga acagcaactg cagcacttca
     3301 agtaccaagt gcagatgcag ctatcgcgat ggaagaagca ttttaccaat tcatcgaagc
     3361 aaatccagta agcggaacga ttgaagcgga caatacgtat gtaaaaatcg aattagttgg
     3421 taaaggcgca catggtgcaa gcccacaatc tggtatcaat gcaggttctt tcttggcatt
     3481 gttcttagat aactatgaat tcctaggatc tgctaaacaa tttatccatg tagcagctgc
     3541 ttatgtccat gaagattttt atggagaaaa actaggagtc gcttatgaag atgaaaaaat
     3601 gggcaaattg acaatgaatg ctggactatt tgcttttgaa gaaaatggta cagaggaagc
     3661 gaacttcatc aatatgaatt tccgttttcc aaaaggtgta acagtagacg gattgcaaag
     3721 tgacatcgaa caaacagtgg ggcaagaagg cgcaacagtt actcgaggtg ctcgcgtaat
     3781 ggaaccacat tacgtaccaa tggatgatcc attggtggct acattattgc aagtctatga
     3841 agaccacact ggagaaaaag gctacgaaca aatcatcggt ggcggaactt acggacgttt
     3901 gttgaaacgt ggcgttgctt atggagcgat gttcccagga tatactgata caatgcatca
     3961 agcaaacgaa tttatgagtc ttgatgactt gttcagagca acagcaatct atgcagatgc
     4021 catctatcgt ttggcgaaat aaaaatacta aaaaa
//
LOCUS       DBNIBO010000176         4018 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.183, whole genome shotgun sequence.
ACCESSION   DBNIBO010000176 DBNIBO010000000
VERSION     DBNIBO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.183"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..79)
                     /locus_tag="AC1Y77_002510"
     CDS             complement(<1..79)
                     /locus_tag="AC1Y77_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HIQ1576047.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNL"
     gene            complement(401..622)
                     /locus_tag="AC1Y77_002511"
     CDS             complement(401..622)
                     /locus_tag="AC1Y77_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576048.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(720..965)
                     /locus_tag="AC1Y77_002512"
     CDS             complement(720..965)
                     /locus_tag="AC1Y77_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576049.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1205..1819
                     /locus_tag="AC1Y77_002513"
                     /pseudo
     CDS             1205..1819
                     /locus_tag="AC1Y77_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2189..3190)
                     /locus_tag="AC1Y77_002514"
     CDS             complement(2189..3190)
                     /locus_tag="AC1Y77_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HIQ1576050.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3349..3615)
                     /locus_tag="AC1Y77_002515"
     CDS             complement(3349..3615)
                     /locus_tag="AC1Y77_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576051.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3605..3961)
                     /locus_tag="AC1Y77_002516"
     CDS             complement(3605..3961)
                     /locus_tag="AC1Y77_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1576052.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1267 a          726 c          534 g         1491 t
ORIGIN      
        1 ttagattgta acgcaggtag taaccaacag cgacaataat gacgtctttt ttgaattgtt
       61 tgcctttaaa atgattcatt actctgtcct ctctgtcttt tttctcaatt ttacactaaa
      121 atagattttt tggaaaactt tgcaacagaa ccgtttttac tatatacttc ttcccagaac
      181 ttgtccgtct ccttcaaatt atctctacct ctcgagttca ttgggtggtt tgcccattta
      241 ttaaacatgg aatcaccttt ctcatactca tatatttatg tcctatttca attatattta
      301 tatatgctta aataaatttg tatcctgaaa tttattgtaa tatgaagctg tattttacat
      361 atagtaacca aagagttaat aaaataaaaa gagcgaacca ttatagatca gctctttttt
      421 cattgctgtt tttaatctta tctagaatca cttcaaatgt ttcaaaggta tttacatcta
      481 actgacataa ccaaagcaag tacttctttt ctttgtaagt ccaatcaata ttacggtaag
      541 aggacattag attatggtac cttgctcttt gttcaactga agccaattcc cagcgttttt
      601 caaatatttt ttcttcttcc attattgatc cctcgcaaga cgctacctca aacttaaata
      661 atgggcagcg aaagtctaat tccttagatc taagtatagc aaacaaatat gaaaaaaact
      721 tagaaggtag taaaagttct tccgtctttt gctttgcttc tattcttaat gtgtttcgtt
      781 atcggtgccc tacaaagatt ttcattaatt acacactcat ttattgttgt gtattttctg
      841 acatttctct cttgatcaat tattttaatt ccatgggcat cctgatagtt cataggagag
      901 gagcggctat cagaaatagt tgcacctatc tcttgaactt cttttacaac ttctggaaaa
      961 tccatccatc ctgcttcagc tgtcaaaagt aataatctat aatatttatc tttccttttc
     1021 aatctaagaa atcgtcaccc cattcttttt cttctattat aaactattta acttttagaa
     1081 ttcaaaaacc gtttgtctat ttagagttta ccctaaatag acaaaaatat aacattgaaa
     1141 aatttagagt tctaacgtat gattagggtg tattataaac gttatagaaa agtagggaat
     1201 aaatttgaaa attgcttata ttagagtttc atccatcgat caaaatgaac aacgtcaaat
     1261 tgaagaaatg aaaaagtttg gcgctgaaaa aatttttatc gaaaaacaat ctggagcgac
     1321 gattagtcat cggccgattt tccaggaagc tttagatttt gtcagagagc aagatatttt
     1381 tattgtagaa gccattgatc gacttggacg caattatgac gagattatcg attcagtgaa
     1441 ttatctaaag aagaagaatg tgcgacttat tatcaccagt ttaccgatca tggctgaagc
     1501 aattggaaat cctctcctag ataaattcat caaagactta atcatttaaa tctcagccat
     1561 gattgctgaa caagaacgta ctgaatcgaa acgacgccag gcacaaggaa ttaaaatcgc
     1621 taaagcaaac ggcgtctata aaggtcgtcc taagttatac agtgcagaca ccaaagatcc
     1681 tcaacgtcgc cttgtctata ggagtatcgt tcaggatctt gagaatgggg ttgctatctc
     1741 aaagattgca acagactaca atgtgactag gcaaacaatt taccgaataa aaaaagaaat
     1801 cgaccaacta attgtatgat ttcttttttg aatcacgtaa ttattaatta gctttgtcat
     1861 caatacagac aatcatttta gattcatcta gctaagttga ttacatttat gttaaaaaga
     1921 atttagttaa cataaatata attgatgcat aaatcgtcct tatttaaagg catttaacat
     1981 tgacaactta gtataatgta tcttatgtaa actaataaga tttgaatttt attttataca
     2041 cactcgataa tagatcaatt ttatggtccc ccgcatttac gattgcacaa gagaaacata
     2101 aagtcgacct attagcattt cttttcaatt caaacattaa aaaagtttat acctctgcta
     2161 ctaaaaaatg aaattatcta tttaaaattt aaaacggtaa atctgattca tccaaattgg
     2221 aaaatggatc agcctgtatt ggagctttgt atttctccat aataacagat atcttttttt
     2281 cattttttaa aaaatacctt ttaaatttct tatagtcctc tgaaatcaaa atatagcgtg
     2341 atggatactc atcaaaagaa gaatcgtatt cttcctttaa aattcccgat ttacttaatt
     2401 tttctagtac atcaaatact ctatcactca agtaactcct taaattgtta tcactttccc
     2461 attgaactac ttttctataa gttgaattat cagttgaaaa gatagaattt tcatccataa
     2521 acgcatatat tagcagcaat ctttcatcat tgtcaaattc tattggatca aaaatatcta
     2581 agttgtcatc aaaatcttta tcagttccgg taactttttt gttaattact gaaggtgtct
     2641 tgtaagaaat agaatctaaa tcaaagcgtt ttgattctaa attattttgt gctttctcaa
     2701 ttaaataatt cacttttttt gtgagaaacc ttctgatatt gtctttgcta tatgttccat
     2761 aaaaaccctt atcttctaaa gagtccctgt acttcttaac ttctatatac tcttcagcca
     2821 aaacttgtgg aactggttca tctaagaaaa aaacccctac ctcgcctttg ctatttttat
     2881 aaaagtaatc aatttctgct atagttcccg atctataatt atcaactgga gaacccaact
     2941 tatggccaaa aatcgctaat aatatatcac tattttctac aatttgttta ttaataacac
     3001 tttgcccaga gctattattt atataagtag gaatcgcatt cgtttcccat cgaattggca
     3061 tcaaaattac ttttgtatta tctgtatttt ctatattcca tctatatatg gtttcttcta
     3121 cttcattcct ctttgctgct atatcacctg gtgatgctat aaatactttt attagattta
     3181 tggttttcat ttattgtacc tttctcttta tgtctcttta aagtaatttt tgtataaaat
     3241 ttattgactt ttacactata ggacaacttt tatctattta gtacaaaaag gcccactaaa
     3301 ctaattgatt agttaggagc ctattacagg tatattttca actcgctttt aagcaaattc
     3361 tactaccagc tttttgccta atgccctagc gatatcatca agtttatcga aagatacgtt
     3421 atcccctctc tcaattcgag caatagtaga ttgagttgta tgtgcttttt gtgcaagctc
     3481 cgcttgagtt aggcctgctt cctcccgtgc atgataaaga gcaaccgcag tttcgagttt
     3541 tcttccttca cggtcatatg cttctgcaaa atctttgtct ttttgctttt cttcaatcaa
     3601 ttttttaatc atcatttgtg aaccactcct ctctaagttc ttttgcatgt ttaagttcac
     3661 ttattggtgt tttctccgtt tttttagtaa atccatgggt aatgacatac cttccattga
     3721 ccacatggaa atagatagct ctttggatgt ttgatcctac cttacttctt aattcgtaca
     3781 aattagtgtc taattttttc acccatttca accgttgagc tactagcatc ccattctttt
     3841 ctgtttcttc tattgtacgt aaaagcttcg cactatcttt ttttggtaat gtttccaacc
     3901 attctaaaaa ttcatcatga ccattaggtc ttttaaatgt ttcaaaagat attttttcca
     3961 tacccaatta tatcatatat tgattttata ttatagtata taacataaaa attcgaat
//
LOCUS       DBNIBO010000177         3916 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.186, whole genome shotgun sequence.
ACCESSION   DBNIBO010000177 DBNIBO010000000
VERSION     DBNIBO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.186"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1332
                     /locus_tag="AC1Y77_002517"
     CDS             <1..1332
                     /locus_tag="AC1Y77_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HIQ1576053.1"
                     /translation="IVTALFIPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGT
                     RGAVLGTIGTIGLIIGADIPMFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNF
                     SIGIIGFILMCLSYLVVGPIIQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNN
                     VIDQGVYYPLGMQQTLEAGRSIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIII
                     HFFGGIHEIYFPYVLMKPLTILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALT
                     PRGNFLGVMAGVVTATLVSFFVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLK
                     EENAREICTEKKEMNKISFACDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVP
                     EDSDLVIVHQDLEERTRKAHPNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            1618..3243
                     /locus_tag="AC1Y77_002518"
     CDS             1618..3243
                     /locus_tag="AC1Y77_002518"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIQ1576054.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         1322 a          551 c          759 g         1284 t
ORIGIN      
        1 attgttactg cattattcat tccaactggg tggttcccaa atgaatctct gaatgaactc
       61 gtaagtccga cagtcagcta tctattgccg ttactacttg cctatactgg tggtcggatg
      121 gttggtggta cacgtggagc tgttttaggt acaatcggaa caataggttt gattattggt
      181 gctgacatac caatgttcat gggagcaatg attatggggc cacttggtgg gtggatgatg
      241 aaaaaaacag atgatttttt agatgggaaa gttcctgctg gttttgaaat ggtcgtcaat
      301 aatttttcta taggaattat tggtttcata ctgatgtgcc tttcttatct ggtagttgga
      361 ccaataatcc aatcagctaa tcaatttgta acgagtgcaa ttgaagcatt agtttcaact
      421 ggttttttac cgatactgtc cttaatcaac gaaccggcaa aagtcttatt tttaaataat
      481 gttattgacc aaggtgttta ttatccatta ggtatgcaac aaacattaga ggcaggcaga
      541 tcaatttatt tcatggtagc atcaaatcct ggccctggct taggactatt gttagcttat
      601 tctttatttg gtaaaaaggc agcaaaacag acagcaccag gtgcaatcat catccatttt
      661 ttcggcggaa tacatgagat ttattttcca tatgtattaa tgaaaccact cactatatta
      721 tctatgattg ctggtggaat gacaggaatc atcacttttg aattatttga tgcgggcctg
      781 gtagcaggtc caagtccagg ttctattttt tcttatttag ctctaacgcc tcggggaaat
      841 tttttgggtg tcatggcggg agtggtcaca gcaacactgg tttccttttt tgtaacatca
      901 gtaattttaa aagcagataa atcaacagat gaagacagaa attttgaact atcagttcaa
      961 aaatcgaaaa cgatgaaatc agaaggaaga aaattattaa aagaagaaaa tgctagagaa
     1021 atatgtaccg agaaaaaaga gatgaataaa atttcctttg cttgtgatgc aggaatggga
     1081 agcagcgcaa tgggcgcaac aatattcaag aaaaaattgc aaaaagcagg tattactgat
     1141 gttgagataa aaaattatcg aattgaagag gtaccagaag atagtgattt ggtgatcgta
     1201 catcaagatt tggaggaacg gactagaaaa gcacatccta acatgaaaat tgtgacatta
     1261 aagaattatt tgcaagatcc agtaatcaat ggattagcag aagaaatcat tggattaaaa
     1321 acagaagact aatttatggg aatttttgat taacctttat taaaattatt ttgcctaata
     1381 taaaaaggtt gtgacatggc ttgtgtcgca accttttttt gtcgcacaaa taattaatcg
     1441 gatattttat gttcatattt tttgtgatca acgagaactg atggggcaaa gatacatttt
     1501 agatactttt aactgttaat ttacaaaaca aggtgaaacg aatagaaaaa tgaaaaaatc
     1561 gatacggaag aatatgtttg attaaaacat gcatgaagag aaggaggttg atgatacatg
     1621 aaaaaaatat attcaaagga aatcgattgt tttagaggca ttgctgtttt agcagtgata
     1681 ttttatcatt tgccaaatag tttgctgcca ggcggattgc aaggagtaac gatctttttt
     1741 gtggtttctg gttttttaat ggctaaaaat acgataagag atgaaaaaaa cagacaattc
     1801 agttttttac atttttacta taagcgtatc aaacgtattt atccattttt actgttttct
     1861 ttggtcgtat tcattttcat actagggatt agcaacattc gattgatagg aaacattcgt
     1921 agtgagctac ccagtttgat ttttggctat aacaattggt ggcagctgaa tcaaggagta
     1981 tcttattttg actcttactt taatgcttct ttatttaaac attattggtc gctttcagtt
     2041 gaactacaat tctatttgat atggccattt ttgtttattg tcattaagcg tatgagaaga
     2101 aaacaagtgt tttacttaat ctatacattg atttttgttt ctattctttt tagtttgttt
     2161 ttaccttcaa ctaaggcata ttatcatacg atagctaaac tttttccatt tttattagga
     2221 gtttgggggt attttaatcg aataactatt ggccgtttct ttgaacaaaa cacttttagt
     2281 aaaatctggc ttttattact agctagtctt tgtctgattc tgttcccgat ttttccttat
     2341 acactaaatg aactactgat cagtatttgt tttgctctat tgttagccag tgtcgatgat
     2401 atgaacattg caaaaaagac caaaatcgct agtacaaaac tttcttttgt aggaaaaatc
     2461 agttatgaac tttatttggt gcatttccca gtattgattg ctctgacaaa tctattcaaa
     2521 catcaccatt ggaaatattt ggatatcgca ggtctattca ctgtgttttt atgtacatta
     2581 ctcatgatta cttgctttag aaaaggagtg caaaagatga gatcacccaa aatttggaca
     2641 gctatggcac taatgataat cggagtttct gtactggtat tacgtgctcc tgccagtcga
     2701 ctgaccgctg accaaaatca gttgcaaagt ttattaaaag aaaactctag taaagcaaat
     2761 gcaggagaaa atctgttgtc acaagagaag aaaatattat ttatcggtga ttctgttatg
     2821 ctgggggcgt atcaggaact aaaggagaca tttaaaaaag aagcattggt agatgcaaaa
     2881 gaatcaagac aaatcactgc gttaccagaa atcttaaagg agcataaaga tttagtttct
     2941 taccaacaaa tagtgatcgg gttaggaacc aatggtgtga taacagatga agctatagaa
     3001 caaacaatgt ctttgctgaa agataaaaaa gtatactggg taaatataaa agcaccaact
     3061 ggctggcaag atacgataaa tgccacatta gctacattgc caaatcagta ttctaatata
     3121 acgatgatcg attggtatag tgagagcaaa aatcatcctg aattttttta tgatgatgaa
     3181 acccatttga atgaaagtgg acgtacagct tatgcaaagt atattgcttc agttattcaa
     3241 taaggtagtg attacgtgtg ttaaccttac tttttcctaa taggtgaatc aatttaaata
     3301 aaaaatactt catatgataa acggtgttta agaggaggag ctttttaata ttcgtggtaa
     3361 aaaagagatg ggcgaggtcg ttaactgaga gcttcttgga aagaaatcgc tttggattta
     3421 tcctctttgt tatacagaac atgagcgaga cgacaatact aggatgtatg ttctgatcga
     3481 agttttatgg ttacaagcag caatcagctt agataaaatt aaggatcgta ggaaccaagt
     3541 aaagaaggct gttagtaaag aaacaaacga tcaagtatgt tttactctta agaagatgca
     3601 gtgaataaat aatagtaata taatgctaga agggactgtg acaaaagacg tgtcacagcc
     3661 ttttttgtta ttttaattat cgtccagtct ttttttataa atgattgctt aagcattaaa
     3721 tattaaaaaa tatagagttc aaaattactg cacaattaga tgaaaaattt gaacaacaaa
     3781 aagtgtattt ttatactata atagcttttg aatttagtta ctctattcaa aagttatgct
     3841 atggacaaga aaaagaataa atagaggtga gaaaatgaat agtttcaaga aaaaatttgt
     3901 gagtagattt ttaaat
//
LOCUS       DBNIBO010000178         3878 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.187, whole genome shotgun sequence.
ACCESSION   DBNIBO010000178 DBNIBO010000000
VERSION     DBNIBO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.187"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(17..1336)
                     /locus_tag="AC1Y77_002519"
     CDS             complement(17..1336)
                     /locus_tag="AC1Y77_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIQ1576055.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1333..1986)
                     /locus_tag="AC1Y77_002520"
     CDS             complement(1333..1986)
                     /locus_tag="AC1Y77_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIQ1576056.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2274..2794
                     /locus_tag="AC1Y77_002521"
                     /pseudo
     CDS             2274..2794
                     /locus_tag="AC1Y77_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2792..3511)
                     /locus_tag="AC1Y77_002522"
                     /pseudo
     CDS             complement(2792..3511)
                     /locus_tag="AC1Y77_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3536..3721)
                     /locus_tag="AC1Y77_002523"
     CDS             complement(3536..3721)
                     /locus_tag="AC1Y77_002523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIQ1576057.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
BASE COUNT         1244 a          733 c          488 g         1413 t
ORIGIN      
        1 caaaattata tcttaattaa gaaaatattt ttattttaaa gataaaaaat tcttcatcct
       61 gcaatacttt atattctata ttgtaattat tcagaatatt acttacaata tataaaccta
      121 atccattact attttcctta tttaaatcaa atttaacatc aaatatttta tccatattcg
      181 aaattttatt gttaccgtat gaattctcta tatataacca atcattaact atcccaatat
      241 taattacccc atttacatca gtatatttta ccgcattact aatcaaatta gatagaataa
      301 tctttaaagc tgtttttcct atataaacat tctcgtctaa tatataatta ttgacagtta
      361 ttttcttttg atttgctaat atttcatact tttttaatat gtcttctaat gtatcattta
      421 ttttcaaata ttcttcatca ttattcaaat tttctataga atgaactgat aatatttgag
      481 aaatattttt tgttaaacta tcaacgattt caatacattc attaatataa atatctcttt
      541 ctttatattt tccaatattg tatttcatgt tttccagtat tattttaaga ctagcaagag
      601 gggttttaag ttcatgggat gcacctttaa aaaagtcata ttttaacttt tctaatttta
      661 aaatttcttt atttttaaat tccaaatcgt caattgttct taataaagta gaatacaaat
      721 cattaatttg ttgttttaat tctccaactt catcattaga gctaactttt aaactcattt
      781 ttttatcaag tttcatcatt ttatcagtaa caatttttat ttcttgtata ttatttttta
      841 ttgattttgc ataaattaaa gaaataatag cagaaaataa aattgatatt aataaagaat
      901 atggtaaaaa tttaaggctt aaatctttcg catctttttg catatcagct gtagaaacaa
      961 attgtagatt tgttttttta ccatcattaa gctttatttc tctttcttca attatcaaag
     1021 aattactatc actttctaaa ttaacattta tattatcttt gatttgtatt tcattcttat
     1081 tatttttttc ttttatgaat acttttattt cactactctt ggaataaaac tccaaagttt
     1141 gttctatata ttttatttct tttccattca tattactaga aatttcattt gccatattat
     1201 gaatcttttc ttttctcgtc tccaaatatg ttttaggaaa gataaaaaaa attaatgaat
     1261 gaactaatat aattattatt ccaagaacag aaaatatttg tatgaacatt tttggaaata
     1321 tctttaattt tttcattttc tctccaattt atatcctaca tttcttatag tagttataca
     1381 gtctaattgt aatttttttc taagttcttt tatatataca tctattactc gatcataagg
     1441 agtttcttcg ctatctttcc atacataatc aataatttgc attcttgtta acacttgtcc
     1501 attattatcc aataaacatt ttaatacttc tagttctttt gcatttacat ctatttcttc
     1561 accatttatt ttagctgtat agctattaaa attaaccgat agatccttat attcaaactt
     1621 ctctaaatgt ccataattct tcttaattaa agaatctatt ctagctttta agaccggcaa
     1681 tgaaaatggc ttttctacat atccgtctac taaattagta aatgcatcaa ttttatattc
     1741 ttcatcacta aatgcagtca agattagaat tggtaaattg cttttctttc taatttcttt
     1801 caacacttct aaaccattta taaaaggtat ctgaatgtct aagataacca aatttatatc
     1861 gctattaaat tttgacaagg cttctcttcc atctttagct tgaataacag tatatccaaa
     1921 ttctgaaaga tattcactta ttccctctct tatcatatta tcatcttcaa cagttaatat
     1981 tttcatatat acctccactt aagcctatac actagatatt atacagcata ttatggctct
     2041 ataatatcta gtgttggtga cgccttctgt tgccaatact ttttcttttt tttaataaat
     2101 gcaaacagac accttgaaat cctttagaaa aaaggcttct ataatatttt gtgttgttgg
     2161 aaaatcgaag gcgattttct actaacactt tttcttcgtt tcaactcaaa aagagactca
     2221 cttttggtaa aatttagtcg accaaaacat caccctacca aaggagtcgc tctatggata
     2281 atcatactag aaaattactc ggattaacag ataaacattt attttttgat gaagaatggc
     2341 tgattgaaaa agaatataaa ggcgttcaag cgcagtttat taaagggaga ttggatgact
     2401 caccttccca ttgtgaacac tgtggatcga ttcgtattat ccgcaacggt tcctatacga
     2461 cacgtactca gatgttgaaa gtcaaagaaa agttgactat tttagaatta aaacgtaccc
     2521 gttttctatg ctatgattgt gggcagacct tttctgcgaa aacggactta gttgatgaac
     2581 atcatcaact aacaaaggaa ctgaagcagg cgattctcat agaactgtac gaaaatcaat
     2641 cacgtaatct gaaaatcaga agaagtaccg tcggctcaag cgatattgga aactcttact
     2701 caaagattcc acgacattgg agccgctcaa acggcattat catcgactgt ttaaacgacc
     2761 aattagtcaa acagaaatcg tcgatgaacc gttgtgcaca atggtgtcct gaacgtacag
     2821 cgacaccttc catatccaga gcagtcgcca catcacgggg atggatgcca tccaaattaa
     2881 aagcaaccac gccagtatga tgttcaggat cttgcggtcc gtaaatggtg ataccgccga
     2941 tcgcttgtaa tttaggtaaa acgtaggcaa ccagttcttg ctcataacga tgaatattct
     3001 ccataccaat ctcattcaag taatcgattg ctcctgcaat gttcggagta ccagcttcaa
     3061 atttccatgg cagctctttc catgaactat cgtaaagatt gacaaaatct atcatttcac
     3121 cgccgaattc gactggctcc atttgttcta acaattgacg tttgccatat aatacaccga
     3181 ttcctgtcgg cccgcacatt ttatgcccgc taaaggcgaa aaaatctgca tccaacgctt
     3241 gtacatcaac tgtcatatgc ggtgctgctt gggcaccgac tggactatcc tcaactacaa
     3301 cagcctgttc aaccctcagc cgtttcaagc acaacaaata accttctgga tctggtttgc
     3361 cgacctgaag atcatcgatc gtcacaaagg aatcaatttt ttccttaatc atcggatgtg
     3421 cttcaaaaaa atagtctagc tcccgcagtt cagaattgag actactccga ttttccagtg
     3481 ctgctgatcc gctgctcaaa atgttcagca tccactggtc tcccaaccgc ttgacttatc
     3541 ccttcataaa aggacagttc tgctgccgtc ccaacacctt gttgatagtc tgcaaaagga
     3601 attttatagc cgaactgctc cagtaaatag gcttcagtaa atagtgatga atactctcgt
     3661 aaaacatcca ttcactgtca atcatcgtcc catcaaaatc aaaaataagt gctttggtca
     3721 tcttctttcc cccttaccca aaacaagcaa aagctgtgtc ctgctctctc tacagtatca
     3781 gcataaaaac tgccgtctga aaagtcaaag gtgaaagaaa tgtggcgcta taatttctag
     3841 gactgatagt aaatcattct atcagtcctt ttgagcat
//
LOCUS       DBNIBO010000179         3817 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.188, whole genome shotgun sequence.
ACCESSION   DBNIBO010000179 DBNIBO010000000
VERSION     DBNIBO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.188"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            352..1041
                     /locus_tag="AC1Y77_002524"
     CDS             352..1041
                     /locus_tag="AC1Y77_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIQ1576058.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="AC1Y77_002525"
     CDS             1028..2236
                     /locus_tag="AC1Y77_002525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIQ1576059.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     misc_binding    2275..2480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="AC1Y77_002526"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="AC1Y77_002526"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIQ1576060.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1258 a          658 c          771 g         1130 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaag
//
LOCUS       DBNIBO010000180         3736 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.189, whole genome shotgun sequence.
ACCESSION   DBNIBO010000180 DBNIBO010000000
VERSION     DBNIBO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.189"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="AC1Y77_002527"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="AC1Y77_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HIQ1576061.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="AC1Y77_002528"
     CDS             1228..2331
                     /locus_tag="AC1Y77_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIQ1576062.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2328..2534)
                     /locus_tag="AC1Y77_002529"
     CDS             complement(2328..2534)
                     /locus_tag="AC1Y77_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576063.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="AC1Y77_002530"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="AC1Y77_002530"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIQ1576064.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT         1132 a          748 c          636 g         1220 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatg
//
LOCUS       DBNIBO010000181         3687 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.190, whole genome shotgun sequence.
ACCESSION   DBNIBO010000181 DBNIBO010000000
VERSION     DBNIBO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.190"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            300..929
                     /locus_tag="AC1Y77_002531"
     CDS             300..929
                     /locus_tag="AC1Y77_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576065.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1053..2162)
                     /locus_tag="AC1Y77_002532"
     CDS             complement(1053..2162)
                     /locus_tag="AC1Y77_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1576066.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2371..2793
                     /gene="mgsA"
                     /locus_tag="AC1Y77_002533"
     CDS             2371..2793
                     /gene="mgsA"
                     /locus_tag="AC1Y77_002533"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIQ1576067.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      2980..3078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            3116..3652
                     /locus_tag="AC1Y77_002534"
     CDS             3116..3652
                     /locus_tag="AC1Y77_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIQ1576068.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         1085 a          681 c          724 g         1197 t
ORIGIN      
        1 gcctgccgtt taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt
       61 gagttagttg gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag
      121 atgtgacaca aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac
      181 ttgatactca cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta
      241 gtaagtgagt tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa
      301 tgagaaaaaa ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca
      361 ttatcccaat ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc
      421 atgtgccatt atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag
      481 gtacgacatt tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac
      541 atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa
      601 aaaatggaca gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg
      661 tgattcattc tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc
      721 ctgaaacata ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg
      781 ggcttatcca cagtttagtt gattataata ttgcttattt tatagcagga acactcagca
      841 aatattttga tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc
      901 aagggtacgc gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct
      961 agtaatggat aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa
     1021 catcttctgg cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt
     1081 tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt
     1141 ttatggaaat ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa
     1201 agcatcgttt cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca
     1261 ggtgaagctt caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata
     1321 acttcttttc cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga
     1381 agcttaagcc catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc
     1441 atggcaggag atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct
     1501 ttcggtgtac cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata
     1561 gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga
     1621 agtttggcaa tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc
     1681 tcatccatta agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg
     1741 cgttgtccac cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc
     1801 tctgctgcat tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag
     1861 ccaaaagcca tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc
     1921 atggcaatat cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt
     1981 tctccttcag agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa
     2041 ccggaaggac caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt
     2101 acagaataat tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc
     2161 ataatacgtt ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc
     2221 gttaactaac ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta
     2281 gttttaagct ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt
     2341 atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga
     2401 aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa
     2461 ctttttgcta ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat
     2521 cggtttaaat ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat
     2581 cagttagatt tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat
     2641 gtaacagcac ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga
     2701 acagctgagg tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc
     2761 catgaaatag acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt
     2821 taatgttcgc ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac
     2881 gaacgtattg gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa
     2941 atatgctata gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg
     3001 ttaagtgcta agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt
     3061 attttcgcat tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa
     3121 gaaacaccgg ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt
     3181 cactttgtcc cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat
     3241 tccacaagtg atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt
     3301 agctgattta gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt
     3361 gaatgcattt attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg
     3421 gaaaaatgcc attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc
     3481 attatggctt gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg
     3541 cctgttaaaa acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg
     3601 tagtattcct tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag
     3661 cagtcttccc atcccagaag actgctt
//
LOCUS       DBNIBO010000182         3628 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.191, whole genome shotgun sequence.
ACCESSION   DBNIBO010000182 DBNIBO010000000
VERSION     DBNIBO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.191"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            230..3508
                     /locus_tag="AC1Y77_002535"
     CDS             230..3508
                     /locus_tag="AC1Y77_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIQ1576069.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1271 a          645 c          779 g          933 t
ORIGIN      
        1 aaaagaagag cataaaacaa acgactttgt tttatgctct tttttatacg ttattttaaa
       61 aaaacttatt ctttttacgt taataaatct ctttattatc aacaacttaa gttatatttt
      121 ttctttttat aaaatacact tattataatt ttgtttttta ttgacagtaa aaataaatta
      181 ggtaatactt taagtaaacg ctttctaatt tgttacggga ggaatacact tgttaaaaaa
      241 acgtataaaa tacagtgcgc taatgatgaa tctccttatg ctcagtaccc cggttttaat
      301 ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt
      361 gaacattgaa gggatgtcaa caatggagga aataccaaga actagtgaag atatggatac
      421 ggaagaagga aatgatccta tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc
      481 tgaagaagca aatgaagagt ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag
      541 gtatgccatt gaaccattag aagacggcgt gcggctctct tccacgaata acggcggaaa
      601 attccagtca agcggatcag atggaatgac ctattattat caagcaatcc ccaaagacat
      661 caactttagg atgcgggcga cgatcgaaat agaatcttgg acctatacga atgcccaaga
      721 aggatttgct ttaatggtaa gagacgcagt accaaaaaat catttgtttg ggcaggcatt
      781 ttattcaaat tcatttgcca tactaggcag tcggatcgaa tatgtctggg actcggatag
      841 acaagaagtg gtcaacacga gcggcagcaa atatacgatg cgacttggat tggggacacg
      901 ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc
      961 agtggaaaca acacctttag agacttcagc agggttcttg gaaaaagaaa caggttctta
     1021 taatatattc ggaaacggtc atgggaatct gtttccggcc acgaactcga ttactaaact
     1081 ggacgtagag atgaaaaaaa cgaatactgg tttagaggca tattattacg atcctgaaac
     1141 aggagaagaa atggcttctg acatcatgta tgactgggaa aaattatacg cttcagatga
     1201 atcggttatt tatgcaggat ttgctgcagc aaggaatatg acgatcaagg tagaaggaat
     1261 cgaagttgct tactcagatc ctgcgacaga tcctcctggg caagaaagac ctactcgctt
     1321 gattgatccg atttacactg tgacctctag taatcattca ggaaaccaag atcatactat
     1381 gtcctttaga gcaaatgctg acggattatt gacaattaaa aataaagcaa caggagaggt
     1441 gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg gaatttgatt atcagacaaa
     1501 actgatggaa ggaacgaacg aatttacttt cagttttacg ccagataaaa actatccgcc
     1561 agaagaatac gtagagatga ctagttatga aacaaaagag attcttcata cagtggacta
     1621 ccgtaaacct aaatattcga cagtttatgt aacaccagaa gggtcagatg caggaaacgg
     1681 aagcagacaa aatccattat ctctgacaca agcattgaga tacgcatatg ctgggcagac
     1741 aatcgtcatg gcgcctggaa cttattcctt cgaaagagga ttgacgattc caaaaggcgt
     1801 taacgggaca agagaaaacc cgattcagtt gattgcagag aaaaatccag aaaacaagcg
     1861 tcctgtactt gattttaaag gaactggaaa tgggatgaca ctgttcagtc actactgggg
     1921 tttacgaggg tttgacatta caaatagtgc agctatgcaa aaaggattac agatttctgg
     1981 aaatcataat ttgatcgaag agattcatgc ttatcggaat ggaaatacag gaatccaaat
     2041 cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg cataacttag tcaagaactg
     2101 tacatctttt gaaaatgccg atccaggatt tgaagatgcc gatggatttg cggccaaact
     2161 gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg tatcataatg cagatgacgg
     2221 ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc
     2281 agttgcttat cgaaacggct gggtaccagg aattgaggga gaaggtaacg gtaatggatt
     2341 caaaatgggc ggttcttctt taacgggccc tcatgaattg atcaatagtt tgtctttcga
     2401 aaatcttgcg aaaggaatcg attccaatag cggaacagat attatcgtca atagcagtac
     2461 aagttttaat aacggttcat ataatgtggc tctttatacc tcttcagcag gaaacacaga
     2521 ctaccatgct tctggtatct tatctttccg aacagagaat cttgagatga gagagcagat
     2581 ccgcccattg aatcaaaacg aagatcaaat ctaccactcg ttgaactact attggaatga
     2641 acgagagaaa caatcagaaa atactcttgg acataccata tcaaaagatt ggatcgagtc
     2701 tctaaataca tgttcgaaag aaggtcagca gcctgttacg cgctatgaaa atggaagtat
     2761 ccatgttcat ggactgatgc agattaaacc ggggaatcgt caaacagaaa aggaaagagt
     2821 ggatggtctt ccattatctg taggtgctat ttttgacgga gaaacctcac cgtcttcgga
     2881 atatccagag tatgcaatcg tacaagaacc aaatcagaca aaaccaaatg aacaaaataa
     2941 tccgattatt atacaagaag caaatggtgt ggcggagaat gcagaaggaa atatcattga
     3001 actgaagcct tttacagttt ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt
     3061 tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt gaaacacagt caagtgctca
     3121 attttctaat cataccgcta ttttttcagc accttcaaaa ggagaatata cgctagaagt
     3181 cacttatcgt tcggaaatat ttgatgggga aaaatggtct gctcatacag aatctgttac
     3241 ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc
     3301 agaaagagaa aatccaggaa atgaatcacc aaatggaaaa ccaaatggaa aaccttctca
     3361 tccggatagg acagcaaaag aaagtgaaaa aaatggcact caaggactcc caaaaacggg
     3421 agaatttaat aacccgttgc tagcattagc tggaggaatt ttgttaatcg gtgtagttgt
     3481 ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc cttttatgat gtaaaacaat
     3541 tgactgagga aagtaaaaat agaaggaaat gagaccaata gatgtctcac ttccttctat
     3601 ctttttatag atgaggttct ccttgctg
//
LOCUS       DBNIBO010000183         3619 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.192, whole genome shotgun sequence.
ACCESSION   DBNIBO010000183 DBNIBO010000000
VERSION     DBNIBO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.192"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            133..1464
                     /locus_tag="AC1Y77_002536"
     CDS             133..1464
                     /locus_tag="AC1Y77_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HIQ1576070.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1430..1780
                     /locus_tag="AC1Y77_002537"
     CDS             1430..1780
                     /locus_tag="AC1Y77_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576071.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1872..2291)
                     /locus_tag="AC1Y77_002538"
     CDS             complement(1872..2291)
                     /locus_tag="AC1Y77_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIQ1576072.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2292..2636)
                     /locus_tag="AC1Y77_002539"
     CDS             complement(2292..2636)
                     /locus_tag="AC1Y77_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIQ1576073.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2986..3582
                     /locus_tag="AC1Y77_002540"
     CDS             2986..3582
                     /locus_tag="AC1Y77_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIQ1576074.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          956 a          653 c          654 g         1356 t
ORIGIN      
        1 ttttttgcaa aattaaaaaa atttggttaa attcaatggg ttcccttaca tttttagttt
       61 tgacctaaat gatagttgtc caaatctttc atttcaagtt atagttaaag aaaatgaaaa
      121 agaaggtgtg taatggaaaa cgaaactgac gttatttata ttcatcctca aaaacgaatc
      181 gtttcacaga aaagaaagta tttttatctt ggttttacag gtgttttttt tctttttatt
      241 ggcttgcttt ctaatactcc tactgataat tggagtggat tgctaactat tcttacctcg
      301 ccaagcaatc ttttgacaga ttattttgct ttaggtgttt tcggaagcgc attcataaat
      361 gtagggattt tgaccttact ttctgtgctt ttagcttatc ggcacaaggt gattttaaac
      421 ggtcccttat ttgcttcgat tctaacagtt accggtttct ctttttttgg caaaaacctt
      481 tataattcta tctcaatcat tttaggtgtg tatctatacg ctgtatttgt caataaacct
      541 ttttctcagt acatcatgat cggactgttt ggtagcgtat tatcaccggt cgttagttat
      601 atcacttttg gtatgcgttt tcctttactg ataggtattt tattggggaa tttagctggc
      661 atagcaattg gtatacttct tccgccctta gctgctcaaa ctttagtttt ccatcgtgga
      721 ttcacacttt ataatattgg attcacctct gggttgattg cgatgacttt tactgctgtt
      781 ttgaggttat tcagctattc aatcgtcgaa aatacattgg ttttcaatga atatcacttt
      841 cctctcattt ggattatttt tggctttttt tcgctgacag tcggaattgg tttctattat
      901 aattcctttc ggctttcggg gattcgagaa attttcgatt catctggaaa attgacaact
      961 gatttcattg ctaattctgg tattggtgct actttgatca acatgggctt agttggtctg
     1021 atgctgtctt cttatgtttt gctggttggc ggtcagttaa atggacctgt catcggtgct
     1081 attttatctg cggtgggttt cagtgctttt ggttgtcatt taaaaaacag ttttccgata
     1141 ctagtcggaa tatttattgc ttctttgttt ggaacttttc atgagatcac cagtactggc
     1201 atgcttgttg cagctgtgtt tgggacaggt cttgcgccca taagcggttt ttacggcagt
     1261 ttttacggtg taatagctgg tgtgctacat attgctcttg tccataacgt cagcactttg
     1321 cacggaggac taaatctata taacagtggt ttttcaactg gtttcgttgc aggtatcctt
     1381 gtaccgatac tagataactt taccgctgtc agaaaggaaa aaaagacact tggaaaaaga
     1441 attatcaaaa agaatcatcg atgagcttct tacttattta tattcccatg aaataactga
     1501 aatacgtatt ggagtagaat ttgctgagga agggcttttc atagagatac agggtacggc
     1561 agaaactcag ccagaagatt tagatgtact tgctgatttg ctgaacaatc ctcgggaccc
     1621 tagtatggaa atctactatc aagaactatt aggtaatccc cgacatgatc gggaagatta
     1681 tcatctgtta ggtagtttga ttgatgacgt ggaaatcatg tatgcccagc cgattatcaa
     1741 tattaagatc tttcgacgtg cttctcattc ctattattaa ccctattttc tatttataaa
     1801 agactgaatc aaaaaagcag ctcctgaaag ttcgattaaa atctactttc aggagctgtt
     1861 caagcttgac attattgatt ttccaatcct tcatacactt catctgttac aggttctaaa
     1921 aactctgaat taccagcagt gatcgccaaa tgttcaaacc aactgtcttt tgtcgcacca
     1981 tgccaatgtt taatcccttt gtgcgttacg acgacatctc cagccattaa tcgtcttgct
     2041 ggctttcctt cttcctgata ccagccttct cctccggtca caagtaagat ttgatagccg
     2101 tctgtatgga catgccagtg attgcgacaa cctggttcaa aagttacatt acctacacca
     2161 acagaaactt cgggatcatt cactaacata ttcagatagc tttgcccggt aaatacttga
     2221 gcgtaagctt cgtttttttc acctgctgga aaaatcacgc cattgtgtat ttcttcgtgt
     2281 tttgtcatca tttacgcctc tttcttatct tcgctatttt cttctaaata gatttctttt
     2341 gctaaactaa aagctgacca agcttttggc cattctacat aaaaagctaa atgggtaatg
     2401 atttctgcta tttcttcact agtaatgcca ttttgcttgc caatcttcag atgagctggc
     2461 aattgttcaa aatttcctcc tgaaattaat gcagatatgg tgatgatact acgctgatga
     2521 gcagataatt cttctctgga ccatacttgt ccaaataaaa catcatcatt taattctgca
     2581 aattttggtg caaaatctcc taattgatct cgtcctgcgg tttgtttttc tgccatttct
     2641 cttattctcc cttcacgact gttaggtgat tcaactatgg ttctaaggca actttatttt
     2701 ctattgtttc ttcgcatttc attttaaatg tagtctttca gcctgtccaa tgcttagttt
     2761 attcagtcga tatgcttttt aagcatagtt gctaacgctt tttttatttt aaaaaaaaca
     2821 agttattaaa taagtcttta gttggatgtt tttttgagat caatctttta tacttatgat
     2881 gaacttacaa aattgtttgt tttctgtcat atcaaaaaat ggcgcagtta gttgcttttc
     2941 tagtaaagta tagttaacaa agaacctcta aggagttgat gaataatgaa tattgctgcc
     3001 tctcgaaaac tgataaattt catatccatt atcggattag gcctatcaat cgcgctgacg
     3061 atttatttta ttaatttagg tgttttcaaa gatttgaatg cgttaagagg attagtaggt
     3121 gattccatca ttttaggacc gattattttt gtcttcatcc aaatcctgca agtagttatt
     3181 ccaatcattc ctggagggat cagtactgca gctggtgtcc tgatatttgg cccttatgct
     3241 ggatttatct ataactatat tggaatctgc attggttcga ttatcatttt tctgctaggg
     3301 cgtcgttatg gaaagccatt catactttcc atgattagcg ataagacata taacaaatat
     3361 attggctggc tggataatca aaaccgtttt gagaagctgt ttgctctagc tatttttctt
     3421 ccagttgctc ctgacgatgc gttatgtttg atggctggat tgacaaacat gtcagtgaaa
     3481 cgatatacac taatcatttt gattgctaaa ccactgtcga ttttcttata tagtatggcc
     3541 ttgatttatg gaggtcaata tctaagtggt cttcttggat aatatttatt ttgtaaaatt
     3601 agtcattgat tgacaatgt
//
LOCUS       DBNIBO010000184         3616 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.193, whole genome shotgun sequence.
ACCESSION   DBNIBO010000184 DBNIBO010000000
VERSION     DBNIBO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.193"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            164..313
                     /locus_tag="AC1Y77_002541"
     CDS             164..313
                     /locus_tag="AC1Y77_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576075.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            394..990
                     /locus_tag="AC1Y77_002542"
     CDS             394..990
                     /locus_tag="AC1Y77_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIQ1576076.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1003..1902
                     /locus_tag="AC1Y77_002543"
     CDS             1003..1902
                     /locus_tag="AC1Y77_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIQ1576077.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1905..2390
                     /gene="ssb"
                     /locus_tag="AC1Y77_002544"
                     /pseudo
     CDS             1905..2390
                     /gene="ssb"
                     /locus_tag="AC1Y77_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2412..2669
                     /locus_tag="AC1Y77_002545"
     CDS             2412..2669
                     /locus_tag="AC1Y77_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576078.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2828..2950)
                     /locus_tag="AC1Y77_002546"
     CDS             complement(2828..2950)
                     /locus_tag="AC1Y77_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HIQ1576079.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3105..3364
                     /locus_tag="AC1Y77_002547"
                     /pseudo
     CDS             3105..3364
                     /locus_tag="AC1Y77_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1295 a          622 c          730 g          969 t
ORIGIN      
        1 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
       61 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
      121 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
      181 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
      241 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
      301 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
      361 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
      421 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
      481 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
      541 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
      601 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
      661 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
      721 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
      781 cgctatggga atcctattat agcttatgat ccagaaagaa ctttatgtga catttggaat
      841 ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta tatggagtcg
      901 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttaccaac agacaaaacg
      961 atgcgttctt atattatggc tttaaattaa aggagaagat acatgacacc tacgcagtta
     1021 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     1081 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     1141 gtgttaaaag gcggcttctt gattggctca aagtatggca ttgaaaatcg cacaaccaaa
     1201 gatattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattaac aacggttcta
     1261 aacgaaattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca aggaatgaaa
     1321 gaaacgagag aagccgatta ctatccagga tttagcttac gtgtgctggc acatttggaa
     1381 aacatgcggc cagattttaa ggtagatgtg actacaggtg actcgatcta tcctgcaacg
     1441 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     1501 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     1561 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     1621 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     1681 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     1741 aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga gacaatggat
     1801 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct
     1861 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     1921 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     1981 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag
     2041 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     2101 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     2161 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaagta
     2221 aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccgcca
     2281 cctttgaaca acggaataca gcgacgccta acaacaatgg attgaacgct tcacagaatc
     2341 catttggtgg acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag
     2401 aaggagtaag catggcaaaa aaaattagtc tgacgatgga tctattgata cccgtaattg
     2461 catttataga cgggatttct ttgggcgtca ctgaaaatcc tttggtaaat agaattgcga
     2521 aaggatcact atttcttgta ttcgtgtatc ttgtctgttc ttatctttta aaaagagcag
     2581 gaaaggataa ttccttttgg cgattgacta aagtcacagt agcagcgaca gctgttatac
     2641 ttacggctat tctaccgctt tatatgtaga gttaacggga aatcgttgaa cttcttgcga
     2701 aatattgcta taatgaacat gaaaaagagg agttgcgcta acaacccctc attaacagca
     2761 ccgattaaag aacggtggca gaatattagt tatttggaaa taaccgctga cctgctaaag
     2821 tagacggtta tttctttttg tcatttttaa gcatatttac aatcaaacta atcaaagcaa
     2881 tggtaaacat accaaaacca agaatggttt gaattgtctc atatgcggac aaaaggctta
     2941 ctcctttcat agattttagc acttacattc ataagcacca ccacctttcc agaagtcgcc
     3001 accgtcttca aactttactg tcttgactat tatagcatat ctaaaatcga ataaaaattc
     3061 tcaattttag ctgtttttgc tataatttta gcgaggtgat cgtgatggaa caatatattg
     3121 gtttactcga taaattaggg tgatcaagac acagccattg cttgtgcgat ttacattacg
     3181 cacgagtcat gaaccaatca attgtgtagt ggccaatata gaaatagcgg ataaattgct
     3241 gattttggaa gaaggcaagt atactatttc agtgacaggt cacttcaata aacgaaatca
     3301 actagtcatt gaatcaatgc aaattagaaa ccctgatcat tttacgagat taatgggcat
     3361 ataaataaaa gaagagctgc agtcctaaat gaggattgca gctctttttg ttttacatag
     3421 aaacaagaaa aatgaaaagg cctttttagg aaggcgcaag cgccttctca attgaaaaac
     3481 ctctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gttgcacgtt
     3541 cactaagata aggggtcttg caaagcaaga caaaatagaa aaaacttcct ctcaccaaaa
     3601 ggttcgttcc tgttcg
//
LOCUS       DBNIBO010000185         3572 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.194, whole genome shotgun sequence.
ACCESSION   DBNIBO010000185 DBNIBO010000000
VERSION     DBNIBO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.194"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            14..193
                     /locus_tag="AC1Y77_002548"
     CDS             14..193
                     /locus_tag="AC1Y77_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576080.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            534..797
                     /locus_tag="AC1Y77_002549"
     CDS             534..797
                     /locus_tag="AC1Y77_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1576081.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            791..1141
                     /locus_tag="AC1Y77_002550"
     CDS             791..1141
                     /locus_tag="AC1Y77_002550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIQ1576082.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1165..1779)
                     /locus_tag="AC1Y77_002551"
     CDS             complement(1165..1779)
                     /locus_tag="AC1Y77_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576083.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            2093..2515
                     /locus_tag="AC1Y77_002552"
     CDS             2093..2515
                     /locus_tag="AC1Y77_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576084.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2525..2728
                     /locus_tag="AC1Y77_002553"
     CDS             2525..2728
                     /locus_tag="AC1Y77_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576085.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2939..3523
                     /locus_tag="AC1Y77_002554"
     CDS             2939..3523
                     /locus_tag="AC1Y77_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576086.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1246 a          523 c          643 g         1160 t
ORIGIN      
        1 ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga
       61 aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg aatctaagat
      121 tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atataccagc
      181 actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac ttaattgttc
      241 ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat aactatgaaa
      301 tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc tgattctaaa
      361 gattttgttt tcttaaacat attatgattt cctttcgact attttcatat aaatgttaac
      421 atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa ttaagtatat
      481 acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct actatggaaa
      541 tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag tatattatgg
      601 aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa gtaaacggtc
      661 agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt gaacagttat
      721 ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt gaagcagtag
      781 gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga atacagcacc
      841 tcgatctgga cacgaacaga caggtaaaag accttatatt gttttaagtc atgatattat
      901 tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta gaaactatcc
      961 gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag tattattaga
     1021 tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa aagcgcatga
     1081 tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag taagcaaata
     1141 aaatagaaac agcagcatga tcaactattc gtgattgacc atgctgtctt tttttattct
     1201 atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg caacaccttt
     1261 ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt ttgcattggc
     1321 actgtatagc ttaggacgtc ctttataaac accgttcgct tttgcaatct taatgccttg
     1381 tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca aaatttgaat
     1441 gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg ccatgatcgg
     1501 tagacttgtg atgatcagct gcacattttt cttcttcaag taattgactg aagcgatgat
     1561 ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat cttgatctcg
     1621 gacgaaatcc agtgcttctt gaaaaacaag tcgatgagta atcgttgcgc cagattgttt
     1681 ctcaatgaaa attctctctg caccgaattt tttcatttct tcaatctgcc gttgttcatt
     1741 ttgatcgatg gatgagacac gtatgtatgc aactctcaaa atttttacct actttctgca
     1801 actatttaaa aaacacccta atcatacacg agaaaaccag atttttcaat gtcgcggtcg
     1861 tgtctagtta gggtatacct tatatagaca aaaactaatg atggaggaaa tagtatgtgt
     1921 cacttttctt tttaaatggc aatatttttt gtaaataaat gtcaatcaag aaaatttaaa
     1981 cgattatgac attaaaaata tattttatga attttctagt atataacttg atttatctgt
     2041 taagatttat taagaaaacg ttttcttatt tttgatttcg aggtgataga caatgaaaaa
     2101 taagtttatt ttatctttag gtattttaac tattggaatt agtgtaactg gaactacaat
     2161 tcctacattt gctgatagta caaatgaacc aactactgta gtagataata accaagaatt
     2221 aactccttta gataggaacg gaatagccat tggtaaccca actaacgact taactgttac
     2281 tgaaaattat ttaatagttt ctccattact tgctcgagca gcttctaaaa cgatacgagt
     2341 gacaggaacg atgatttttg ataaagttgg ttggccttct agacctcctc aatcacggaa
     2401 ttggaaagaa aaaagaaatg gttattggta ctatggaaaa ttgtatttac aaagttataa
     2461 atcagctgga aacaattgga ttgcgactta ttcaggaacc ttaactagaa agtagggatt
     2521 ttttatgcca gaatttgtga acacaccaaa tttttatcaa gatagcgata ttctaagtaa
     2581 aattaattta ccgaaagaag aatcagcttt acaagttcag cgaactttac aggagacaca
     2641 aaaaattcaa aaatcaattt ctaatctaga agattctgaa gaaaaaagaa aaaaaagaga
     2701 ccaaaaaaca tatcaaaaag aaaaataatg attttaaaaa aagagtgtct agtcgacatt
     2761 ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt tctttttttg
     2821 ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa tatctatgtt
     2881 cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag tgattttttt
     2941 gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac aagaagacag
     3001 cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg caaaaagtaa
     3061 tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac ttgtcgtttg
     3121 gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca atcgactgaa
     3181 tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca aatcaagttc
     3241 tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc gtaatttgat
     3301 cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt ttggtggtcg
     3361 tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg atagcggtac
     3421 acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt atcgttattt
     3481 ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt catttaatgc
     3541 tgaaaatttt tgccatttat ctagaaaagt ga
//
LOCUS       DBNIBO010000186         3428 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.195, whole genome shotgun sequence.
ACCESSION   DBNIBO010000186 DBNIBO010000000
VERSION     DBNIBO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.195"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            102..713
                     /locus_tag="AC1Y77_002555"
     CDS             102..713
                     /locus_tag="AC1Y77_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIQ1576087.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            786..1715
                     /locus_tag="AC1Y77_002556"
     CDS             786..1715
                     /locus_tag="AC1Y77_002556"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIQ1576088.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1852..2610
                     /locus_tag="AC1Y77_002557"
                     /pseudo
     CDS             1852..2610
                     /locus_tag="AC1Y77_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740602.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2658..>3428)
                     /locus_tag="AC1Y77_002558"
     CDS             complement(2658..>3428)
                     /locus_tag="AC1Y77_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIQ1576089.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1136 a          530 c          654 g         1108 t
ORIGIN      
        1 gatataataa aatgtattat aggcattttt ttacaagaaa tagaagcttg aagtttatat
       61 gaagttataa aatttaattt atattggaag gaggaaaata aatgtatttt atactaccta
      121 cactttatag ccttttatgt gtaatggcac catgtattat ttatatggta tttttttgca
      181 aaaaaaatac cctgaaacac aatatcctga ttttgatttt tatgctgtac tgttggaaag
      241 tttttgaatt gactggtaca ggtggactaa cagatatttt ttatagacct gatacaggta
      301 ggaaattttc tctagtacag ggcagtgtta atcttgtacc attcagtgga ctaaacatta
      361 gctttatatt aaatatagta atgtgtgtgc ccttaggatt cttactacca atcatatgga
      421 aaagttatag gaaaatagtt cctgttttct tggctggggc tctattttca ctaattattg
      481 aagtctcaca aattataact acaagagcaa cagatattga tgatttaata gcaaatacct
      541 ttggtgcggt tataggcttt atactgtggg ctgttttaag tagagtggtg tccttgaagg
      601 acaagtcatc tacagtagat tatttagaac cttatattta tatattgatt ggttttaccg
      661 gaaaattctt actgtatttt cctttctggt ttgcttttga agtattatcc tgataattcc
      721 atagaaaata atgagtttga gttgagaaag aatattcttg tacacaactc tggaaaggag
      781 gaagtatgaa aagattctta aaatgggggc ttgttctttt agcgtctttg attgtgattc
      841 taatgtgtac agcggttgtt atttcttatg tgaatcggcc gtatgcaaga gctgtttatg
      901 ataggattaa acgcattctt gacaaagatt tttatgacca tctatatcta ggttcttcat
      961 ttgaaataat aaaccttgat acaatggata ttcatagcta tatgaaaaaa gaccaagaag
     1021 aattacccgc ctcattgagc aaattattta cgattgatta tgcgcttagt gtcctaagtt
     1081 tagatgaaca gattacaatt agtcctactg cacttgacat gcagaaagct gactcatcta
     1141 ctgcacatct tcaagaagga gatattttat ctgtttatga cattatctgt gctatggtct
     1201 taccctcagg aaatgatgca gcttatagtt tggcaattta tacaggtgga aaacttgatc
     1261 ctgatgcaaa gaatgatgaa gaacgctatc aaagcttcat gaaaaactta gatcgttatc
     1321 taagtgagca aggctataaa catactcatt ttactgacgc tgctggctat gactccaatg
     1381 ctacaacatc agcggaagat ttgacaaaag tagctgcaaa gctactaaag aatgataaat
     1441 ttgcagaaat agttgccaag gaaacttata gagcaaaatc aattaaggga aatgtttata
     1501 actggaatac tacgaatcaa tttatgcaac aagataatag atactttact gaaggtgtaa
     1561 aaggtgtaaa aactggatcg tttgatggga gatacaatct atgtgtgtat tatacatcta
     1621 gttctgataa ccactattta cttgtaagtt ttggttcaga gagtgatagt aggcgttatg
     1681 tagatgttca aaatctattg cataatctgc cttgaaattt cgtgccttta agcgcgtatc
     1741 taaagtttct ttgaaaagtt tgagaatgaa gaaagtgagc agtcgaaaaa cggttgctca
     1801 cttttttgcc ctaaaatcga aaaaatataa aaaagtcctt tacaacacgt ctcaggaaaa
     1861 actgctaagt caattctaat atcttgtgga gcaagacttg caccttttga tataagagag
     1921 cttagagaac taatgagcga agatgaacta gaactcgata aaattggaga tagaaaaact
     1981 gccttgtttg taataatatc agatacagat gatacattta attttgtagt ttcaataatg
     2041 tattctcaat tatttaatct actatgtgat aaggcagatg atgtgtatgg tggaagactt
     2101 ccagttcatg ttaggtgtct acttgatgag tttgcaaata tcggtttgat tcctaaattt
     2161 gaaaaactaa tagcgacaat tcgttcaaga gaaatatcgg caagtataat actgcaagca
     2221 caatctcaat taaaagcaat ttataaagac catgcagata caatagttgg taactgtgac
     2281 tctacacttt ttctaggagg aaaagaaaaa acaacagtaa aagaattatc agaaacctta
     2341 ggaaaagaaa ccatagacct atataacaca tcagaaacaa gatcaaatca aaaatctttt
     2401 ggtctaaatt atcaaaagac tggcaaggaa cttatgagtc aagatgaaat aactgtaatg
     2461 gatggtggta agtgtattta ccagctaaga ggagcaagac cttttctatc tgataaattt
     2521 gatattacaa agcataagaa ttacaaattg ttggaagatt atgataagaa aaacttgttt
     2581 gatgtagaag agtatttaaa acaaagatag cgtaaaatat tttgtgtaaa taactcgtcc
     2641 ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt cacttgttgg
     2701 aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat ggatactagg
     2761 aaacgctcta gtgattcttc attttgaaac tgctcttttc tgtggctgta tttcttgatt
     2821 tgcttattaa aggattcaat caagttcgta gagtaaatag tcttgcgtat ggctaatgga
     2881 aaatcataga aagtgagcag gtaatcgttc gaaaggatcg attccaccat ttttggataa
     2941 ctggttttcc atttttccgc aaaagcacca cgtgcttcca atgccgcctt tttagatgac
     3001 gcttgataca ccattttaaa atcatcacag acttccttac gatcatcgac acgcactttg
     3061 tgactgatat tacgggaaac gtgcacacag caatgttgaa aacgggcttt ggggtagaac
     3121 cgctgcacgg cccctgccat cccctttaag ccatccgtga tgaaaaggag gacgtttttc
     3181 acgccacgtt cctggaggtc tattaaaatc tcttcccaaa tcgtgataga ttcagttggt
     3241 gcaatcgcat agctcaacac ttcctttgaa ccgtccgggc gaatgccaac tgcgatatga
     3301 atggcttcct tggcgacggt tttccgcttt aaagggatat aggttgcgtc catataaata
     3361 gcggcataac ggtcatgaag ctcacgttcc ttaaatgaag tgacttcttc cgtaaatgat
     3421 ttagtcat
//
LOCUS       DBNIBO010000187         3374 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.196, whole genome shotgun sequence.
ACCESSION   DBNIBO010000187 DBNIBO010000000
VERSION     DBNIBO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.196"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            254..580
                     /locus_tag="AC1Y77_002559"
     CDS             254..580
                     /locus_tag="AC1Y77_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576090.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="AC1Y77_002560"
     CDS             complement(697..1041)
                     /locus_tag="AC1Y77_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576091.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="AC1Y77_002561"
     CDS             complement(1117..2364)
                     /locus_tag="AC1Y77_002561"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIQ1576092.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="AC1Y77_002562"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="AC1Y77_002562"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIQ1576093.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          670 g         1051 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agta
//
LOCUS       DBNIBO010000188         3349 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.197, whole genome shotgun sequence.
ACCESSION   DBNIBO010000188 DBNIBO010000000
VERSION     DBNIBO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.197"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..243
                     /locus_tag="AC1Y77_002563"
     CDS             <1..243
                     /locus_tag="AC1Y77_002563"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HIQ1576094.1"
                     /translation="CTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQK
                     GSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            259..408
                     /locus_tag="AC1Y77_002564"
     CDS             259..408
                     /locus_tag="AC1Y77_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576095.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            482..1075
                     /locus_tag="AC1Y77_002565"
     CDS             482..1075
                     /locus_tag="AC1Y77_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIQ1576096.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1091..1990
                     /locus_tag="AC1Y77_002566"
     CDS             1091..1990
                     /locus_tag="AC1Y77_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIQ1576097.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1993..2478
                     /gene="ssb"
                     /locus_tag="AC1Y77_002567"
     CDS             1993..2478
                     /gene="ssb"
                     /locus_tag="AC1Y77_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIQ1576098.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2844..3104
                     /locus_tag="AC1Y77_002568"
     CDS             2844..3104
                     /locus_tag="AC1Y77_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576099.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         1228 a          564 c          649 g          908 t
ORIGIN      
        1 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
       61 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
      121 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
      181 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
      241 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
      301 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
      361 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
      421 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
      481 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
      541 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
      601 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
      661 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
      721 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
      781 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
      841 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
      901 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
      961 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     1021 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     1081 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     1141 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     1201 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     1261 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     1321 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     1381 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     1441 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     1501 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     1561 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     1621 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     1681 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     1741 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     1801 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     1861 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     1921 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     1981 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     2041 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     2101 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     2161 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     2221 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     2281 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     2341 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     2401 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     2461 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     2521 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     2581 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     2641 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     2701 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     2761 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     2821 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     2881 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     2941 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     3001 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     3061 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
     3121 agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     3181 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     3241 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     3301 agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcg
//
LOCUS       DBNIBO010000189         3217 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.199, whole genome shotgun sequence.
ACCESSION   DBNIBO010000189 DBNIBO010000000
VERSION     DBNIBO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.199"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1437
                     /locus_tag="AC1Y77_002569"
     CDS             <1..1437
                     /locus_tag="AC1Y77_002569"
                     /inference="COORDINATES: protein motif:HMM:NF012307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HIQ1576100.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEI
                     KASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKI
                     TSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALK
                     PNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEG
                     TIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1775..>3217
                     /locus_tag="AC1Y77_002570"
     CDS             1775..>3217
                     /locus_tag="AC1Y77_002570"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIQ1576101.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKTLLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          581 g          947 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgaacat
     2581 gattttgcat ggggttccca ttgccaacca acacttgcat aatgcggata ccttggatca
     2641 agactggcca acagaagagc caacgaactt cgatggggtg ctcatgaacc caccgtattc
     2701 tgcaaaatgg tcggctgata aaggcttctt agatgaccca cgcttctctg cctatggcgt
     2761 attagcccct aaatcaaaag cggactttgc tttcttatta catggctact atcatttgaa
     2821 agatacgggc gtcatggcca ttgttttgcc acatggggtg ctctttagag ggggcgcaga
     2881 agggaagata cgcaaaacct tgttagaaaa tggtgccatt gacaccgtca ttggcttacc
     2941 tgctaacatc ttctttaata cgtccattcc aacgaccgta attatcttga agaaagaccg
     3001 tgcgaaaaaa gatgtgctct ttattgatgc gtcacaagat tttgaaaaaa taaaaacgca
     3061 aaatacgcta cgagattacc atattgacgc tattttagag gcctataaaa cccgtacaat
     3121 taaagacaag tatgcttatg tcgcagaatt tgatgaaatt gtggagaatg attacaactt
     3181 aaacattccc cgttacgtgg atacctttga agaagaa
//
LOCUS       DBNIBO010000190         3133 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.200, whole genome shotgun sequence.
ACCESSION   DBNIBO010000190 DBNIBO010000000
VERSION     DBNIBO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.200"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            208..1041
                     /locus_tag="AC1Y77_002571"
     CDS             208..1041
                     /locus_tag="AC1Y77_002571"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIQ1576102.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1143..2843)
                     /locus_tag="AC1Y77_002572"
     CDS             complement(1143..2843)
                     /locus_tag="AC1Y77_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576103.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
BASE COUNT         1110 a          519 c          623 g          881 t
ORIGIN      
        1 gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag
       61 tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt
      121 agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt
      181 ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg
      241 cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat
      301 tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct
      361 gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa agataatttt
      421 gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa
      481 atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca
      541 ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta tcagacacgc
      601 aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac
      661 ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac
      721 tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac
      781 tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc
      841 gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat
      901 acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat
      961 caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc
     1021 gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa
     1081 aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt
     1141 gcctaagcat gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag
     1201 ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg
     1261 agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt tacttcataa
     1321 tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata
     1381 tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt
     1441 tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct
     1501 tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg
     1561 gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa
     1621 gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg
     1681 atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt
     1741 tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg
     1801 aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc
     1861 gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt
     1921 ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc
     1981 aatataggaa aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt
     2041 tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct
     2101 aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga
     2161 agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac
     2221 atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca
     2281 gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat
     2341 aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata
     2401 gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca
     2461 tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga
     2521 atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa
     2581 aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga
     2641 aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg
     2701 aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc
     2761 aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg
     2821 caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat
     2881 tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg
     2941 atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga
     3001 aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta
     3061 ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct cttattactc
     3121 aaagcataac gca
//
LOCUS       DBNIBO010000191         3070 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.201, whole genome shotgun sequence.
ACCESSION   DBNIBO010000191 DBNIBO010000000
VERSION     DBNIBO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.201"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            24..635
                     /locus_tag="AC1Y77_002573"
     CDS             24..635
                     /locus_tag="AC1Y77_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIQ1576104.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            620..2011
                     /locus_tag="AC1Y77_002574"
     CDS             620..2011
                     /locus_tag="AC1Y77_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIQ1576105.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            2227..2541
                     /locus_tag="AC1Y77_002575"
     CDS             2227..2541
                     /locus_tag="AC1Y77_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576106.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            2534..3028
                     /locus_tag="AC1Y77_002576"
     CDS             2534..3028
                     /locus_tag="AC1Y77_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576107.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
BASE COUNT         1188 a          427 c          577 g          878 t
ORIGIN      
        1 aaaaaaatgt gaggggataa tcaatggatg ataaacaaaa aatgtacgaa aaaaatcgca
       61 aacatagtga ggccttaaat tttcgtgtcg ataaaaagac ttatcttgaa ctgacattga
      121 ttgcaaaaca aaggggaatt tctagagcag acgtttgccg tgagcggtta aagggactca
      181 aatatgaacc gccgttgtta gctgaagatg atagggtagt tttaaccaaa cagttaaatc
      241 aaattggtaa caatttgaat caagcggtaa aggcattaaa tatcattgct aaatatttta
      301 gagaagaatc tgaagataaa aaagaaaaga gcctacattt tagttttgat gctagccgtt
      361 acaacatttt taaagatgaa cttgcgaata gcatagcaaa aaagttgatg attagttatc
      421 cagatgacat ttttttggag tccaatcgga gtgccccctc tttttttaaa ttatcagaag
      481 tagaaaaaga atatttcgaa tattggatag aaaagcaaac tagaccgggt acgaaagaag
      541 agtattccca aaaagttgat cggaatttaa ttcactcaat tcagattatt gcaagtttaa
      601 aagatgaggt gaaacaactt tgggagctac tataacatcg aaaggacgca agaaaaattt
      661 atcctcgcta ttacaatatg cagctaatcc ttcaaaaaaa gaaaaatgtg ttgcagtctc
      721 aggaataaat tgtagtgatg atagtgagga agcactgcaa gaaatgaatt tagtacgtac
      781 caagatgaat aaatctaaag atatagcaca agcgtttcat gttattcatt cgtttgataa
      841 gagtacttct aaagagttaa gtattgataa aatgcacgaa atagctgtcg aatttgcaga
      901 gaaagctttt ccaaattcgc aaatcattgt tgcaagtcac aatgataaag atcattttca
      961 tagtcatctt gtcattaata atattaacat ggaaacatgt aaaaggttgc gtattgaccc
     1021 taaagattta gaactaatgc gaaaaattaa cgatcaagtc ctcaatgaac atggtcttga
     1081 atcagtgaat aatgagtact atgaaaactt agaaaagaga accaatcttt attcaaacga
     1141 tccttctaag atgaaatatg gaaaaattaa ttatcaagag tttgtgcaaa atgcgatcga
     1201 agcaacgttg gaaaaatgcg aagtgaattc ttttgaagat ttttcggatc atttggcaaa
     1261 agaatataac attgaagttt atcaattttc aaaaacaaat aacaaacttg gctatgttct
     1321 ttacaaacaa ggtgtaaatt tctatgagcg tagaggtgag ttaattactt taggaagaga
     1381 taaacaatct agaaaagaaa aaagtcagta tgtcgaacgt acttttagtg caagaaagtt
     1441 agggaaaaga tattcatttg aatctgtttc tgaacaactt gaaaagaatt tagaagaatc
     1501 acatataaaa aattttttta atatggaaag agagcaagtt atgtcgctaa ttagtggagt
     1561 aaattacaaa actggtgagt taaatgtacc gtttgtggaa ttagattcca tcgcacaaaa
     1621 gcaatttagc gaccgtgaag gcgatgaaaa attcattaaa tatgatgact tcttgcgaga
     1681 ggtagacaag cattttgtaa atacaatgtc tttagaagat gatcaagaat acacgattaa
     1741 acttcatacg gatgtgaatc ctcaaacttt acgtagtgcg aaaagtaaaa caatttctat
     1801 taaaaagact gatcagaaag agttatttct accggcaaag ggttttgaaa attgttctca
     1861 tacaccttct gctaaacaga ttcactatca aatcagtcgc ataatgaatg acgatgtaaa
     1921 aatgtatcgt gctaaaaagg attatgaatt cacacaacaa atgattgaaa atcagcaaaa
     1981 aaggcaacaa aattttgaaa tggaattctg aatgggcttg ttagtggact ttccagtttt
     2041 ttgcctatga agagttgctt ggaaagaagc agtttttagg acatacgcca gctacccatc
     2101 tacttgccag tcaatccgcc ccgctcacgc taggctactt tctggcaagt cagatggcgg
     2161 gtaaatctct gagggagcaa gctccctaca gactccctcc caaaaaaaga aaggtggaag
     2221 aaaaaaatga actatccata ttttaaagtc tcggcttcag aagaaactaa agaaatattc
     2281 aacaattttt acaaccaaaa caaaggaatt tttggttcaa aggcaaatat gtttcgtgtt
     2341 atggtttcaa atttaccagt gttagctagt ccatcaaata ataaatttaa tgatcctgaa
     2401 agcattaaat ttgaacaaaa aatttcagaa ctagaatcaa tgataagtaa tgaagtaatt
     2461 gaaaagctgg atgacattga tcaaaagtta tcgtattctc taaaaaacaa atacaaaacg
     2521 gaggagaaaa aagatgttta aagtttactt cccgtttcag tggctcgtta aaggaattct
     2581 tttagtgcta ctaggattga ttctttttgg aagttatcta acaattaaag ccagacattt
     2641 tccagaagaa aagccaataa aaattgtaac aaaagattta aaaataggtg aaacttatca
     2701 ggcagatggg gttgaactaa catttaaaaa tattgaagtg accgaaaaaa gaaatgaatt
     2761 tgcacaacat gatcgtgtat tacaagtgac ctatacactt ataaatgata cagaaaatcc
     2821 gatacgtcca caacaatggt cgattgaaaa taaggatggt gaagaacttt atgagtatga
     2881 tatgatattg gataatccag agtatattga aagtggcaaa gaagaaacag taaattacta
     2941 ttatgaatta ccgattgaaa ggcaggacta ccaaatcgta gtacgtagag agaatggtta
     3001 catagttggg ttgccattta ctttataagt tttattttgt tagtagtttt taaggtaata
     3061 tcagttttgt
//
LOCUS       DBNIBO010000192         2978 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.202, whole genome shotgun sequence.
ACCESSION   DBNIBO010000192 DBNIBO010000000
VERSION     DBNIBO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.202"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            191..445
                     /locus_tag="AC1Y77_002577"
     CDS             191..445
                     /locus_tag="AC1Y77_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1576108.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="AC1Y77_002578"
     CDS             446..763
                     /locus_tag="AC1Y77_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1576109.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="AC1Y77_002579"
     CDS             1322..2257
                     /locus_tag="AC1Y77_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576110.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2351..2978
                     /locus_tag="AC1Y77_002580"
                     /pseudo
     CDS             2351..2978
                     /locus_tag="AC1Y77_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT         1082 a          432 c          547 g          917 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct
     2281 aaacatgttg gtaaatcaac aaaaaaacgc atagagtagt ctcaaaactc ccaagaaatg
     2341 aggactacat atgcgttcat caaaatatac tgaacattat acagatactt ttataacatt
     2401 caaggaaatt atgagaactg tttctaacat ataccacaat tgtgtaccag ataagattaa
     2461 aaaccgaaga aatactgatc aactgaagta acgcgataca gtgattattg cttgtgttat
     2521 atggggcata attaatggct atactagcca aagagccacg tatagagcgg tttgttccgt
     2581 cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa gttcaaactt
     2641 agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca aaggtgaact
     2701 agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa atagacaagc
     2761 aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat cttattttta
     2821 tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt actcaatcac
     2881 gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag cgaatcttcc
     2941 caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DBNIBO010000193         2968 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.203, whole genome shotgun sequence.
ACCESSION   DBNIBO010000193 DBNIBO010000000
VERSION     DBNIBO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.203"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(84..1031)
                     /locus_tag="AC1Y77_002581"
     CDS             complement(84..1031)
                     /locus_tag="AC1Y77_002581"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIQ1576111.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="AC1Y77_002582"
     CDS             1277..1741
                     /locus_tag="AC1Y77_002582"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIQ1576112.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="AC1Y77_002583"
     CDS             complement(1803..2846)
                     /locus_tag="AC1Y77_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIQ1576113.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          981 a          587 c          567 g          833 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtttcgaa
//
LOCUS       DBNIBO010000194         2960 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.204, whole genome shotgun sequence.
ACCESSION   DBNIBO010000194 DBNIBO010000000
VERSION     DBNIBO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.204"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            97..2814
                     /locus_tag="AC1Y77_002584"
     CDS             97..2814
                     /locus_tag="AC1Y77_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HIQ1576114.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPT
                     RLSDRKDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
BASE COUNT         1101 a          396 c          627 g          836 t
ORIGIN      
        1 ctgaaaatga caattgtgta actcatatca taattgacag aaaaacagct cagaagctct
       61 ctgagagtct cagaaaatgg gctgagggag gtaataatga aaatagttaa agaaaagatt
      121 gccagtagaa aaatacactg gttagctttg agcatacttg ttttaatgtt tatcgttgtt
      181 ttcaccggta atgttgtcca agcaagcatt ttcaattggt ttgatgacac ggaaagtcaa
      241 actgaatttt tgaataatcc tgtctatcaa tcgtatttaa aaaaatataa cggaaatttt
      301 tacagtcagt ttggattgtg gatagggtgg tctgttataa aatcgatgtt tgcaattgta
      361 gatggtattc aaaatatgat tcccgaagtg ttagatttgt ttagctttat tgaaagtacg
      421 ggaatgaata aagtttatca atcggtctta agtacagttg ttattggttt aatgatactt
      481 tcactgatgt ttgttggtta caaaatgatt actggaaaag gaacagtgga tttaaaatcg
      541 gttggaatga acgtagtgat gtctgtagca ttaattcttt taatgccgac tttgattgga
      601 tcaacagaaa aactttcagg aataatgttt gcaaagactc tttataatga cgcaactagt
      661 atcaataatc aagatggcgg tgtagcatgg tcattgattg agcagggagt gacagattta
      721 gcttatatta ataagacaga tcaatattcg acaattgata aaacaaatga ccgaaataat
      781 ctaacaaaag aaaatttctg gaaaacggat cttacgcaag tattgacaga taatgtaatt
      841 gataaaatgg aaaaagagaa cccaacagct gataatctgc gttatgaatt agttgaaaat
      901 tctgataatg aatttgttgc tactaaattt tcagataatt ttttatcaac ctttgttgat
      961 ggtatcaaat caggatatta tcgctatcaa gcgaacctgt ggggaatttc ttttggattg
     1021 attgctttag cggtagcgta tgtttttagt gctttcatta taattacagc aatactcgag
     1081 ttagcattta aacgtgtact cggagtttta gtatttgcta ctgacttaga aacaggacaa
     1141 cgatcaaaac aagttttgtc agatatttta cagtgctatt tgacagtcgg atttcaaggg
     1201 ttcggtttat ctatgtttgc aatgttcata aattacctaa atactggcga aggtatctca
     1261 gcaaatattt ttataaaaac aattgcttat atctgtgctg tgtttgtttt aatcaaaggc
     1321 agtggaacag tcatgagata ctttggtgtg gatattggac tcaaagaagg ctatgggcaa
     1381 cttgcttctg cttttggaat gggtgcaatg ctattccgta aaggaacagg tggtatgaac
     1441 cggtctaaag gtgggaaaaa taatggtgga aacgatcaag ctgaagggga agaacgaaaa
     1501 cctgaacgca actttggaca atcactttct aaaaaagcta ataaaacggg gaaagcgctt
     1561 gggtatgctc atgaacgtgg attatcaggt ttagcttcag atggatctac aatggctagt
     1621 gaaagagcaa caaaaccatt taagagtatg cgtgatatag ctaatgatac caagagtaag
     1681 tttaaagaag gtttagatga tggtactgtg tccgctatta ataaaaatag caaacctatg
     1741 ttagctaaaa ataaagaaga tgaaactgga aaatatgctg attccgtgcc aacacgtctt
     1801 tcagatagaa aagatggtgc taaagttgaa aatgccgata gaattatgtc tagttctgaa
     1861 cgtatgcgtg aagcaatgaa aaataatgct gaatcgaata ataatcctgt aagtgcaatt
     1921 cagcaaaaag tccaacaaga tattgaagaa cgtaaaaatg ctatgcacgg tcaagctaag
     1981 tctgctgaag aattaattaa tcagaagcgt catgaagcga aatacacgcc tgaagcaatg
     2041 aatcgcgagg aaataatgtt aaggaaacgt gtagaagggc ggacaggcgc taatattgac
     2101 ggaaaagaag cgttgaccaa agaaagaatt caagaagcta aaaattcaaa tacaggatta
     2161 gaaaaattag tcaaagaaaa tccacaaaat tctacaagta gtcatggtgg ccgatcagta
     2221 gatgtccgag aaaatattca aggttcactg aatggtcatg ttggacggaa tgttgatgta
     2281 agagagaatt tacaaagttc ttcaaatgct caaggtacta agacaattga tgttcgagaa
     2341 aatctgcaaa gtagcagtac aagtcaacca aaaacagtag atgttcgaga aaatgtacaa
     2401 aattctacaa gtagtcaagg gtttaaatca gtggatgtcc gagagaatgt gcaaggtagc
     2461 agtgcaagtc aaccaaaaac agtagatgtc cgagaaaatg tacaaagttc tacaagtagt
     2521 caaggattca aaacagtaga tgttcgggag aacattcaaa aagatagtgg agaaatgaaa
     2581 gaaaaaacac aaaaaatcaa tatcgttgaa gacagaaagt cagcgaaaaa atttgattca
     2641 aatcatgaaa cagtgattat tgatagtgaa atccgtgaaa atgataaggg atcaaaatca
     2701 agacgaagat tcacttatga agacaatgag ttattcagag atacacttaa tgatcctaat
     2761 ccacttttcg ataatttatt aagaaataaa agggggggat tgtcatgcaa ttaattctaa
     2821 agattagtac gcatgatttc ttagaacttg acgaattatg tgaatcagta aagaacgcaa
     2881 caaatgaaaa aatcacggtc actaattata ttgaactgat gattcaagaa gaattaaaga
     2941 tgaaaaatga aatagagagc
//
LOCUS       DBNIBO010000195         2924 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.205, whole genome shotgun sequence.
ACCESSION   DBNIBO010000195 DBNIBO010000000
VERSION     DBNIBO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.205"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(98..193)
                     /locus_tag="AC1Y77_002585"
                     /pseudo
     CDS             complement(98..193)
                     /locus_tag="AC1Y77_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
     gene            289..606
                     /locus_tag="AC1Y77_002586"
     CDS             289..606
                     /locus_tag="AC1Y77_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIQ1576115.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="AC1Y77_002587"
     CDS             complement(697..1062)
                     /locus_tag="AC1Y77_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576116.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="AC1Y77_002588"
     CDS             1270..1752
                     /locus_tag="AC1Y77_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576117.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="AC1Y77_002589"
     CDS             1949..2839
                     /locus_tag="AC1Y77_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HIQ1576118.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1014 a          451 c          547 g          912 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attg
//
LOCUS       DBNIBO010000196         2909 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.206, whole genome shotgun sequence.
ACCESSION   DBNIBO010000196 DBNIBO010000000
VERSION     DBNIBO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.206"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..151
                     /locus_tag="AC1Y77_002590"
     CDS             <1..151
                     /locus_tag="AC1Y77_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576119.1"
                     /translation="IGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGNE
                     IKIGN"
     gene            167..1009
                     /locus_tag="AC1Y77_002591"
     CDS             167..1009
                     /locus_tag="AC1Y77_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576120.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1072..2874
                     /locus_tag="AC1Y77_002592"
     CDS             1072..2874
                     /locus_tag="AC1Y77_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIQ1576121.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1062 a          389 c          598 g          860 t
ORIGIN      
        1 tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg taaccttaga
       61 tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt ttatattgcc
      121 tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga aaatcataac
      181 aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca aaccacaacc
      241 tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga gtttattaaa
      301 agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat attggtgcat
      361 tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa caatccataa
      421 acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt ttgtaggtgc
      481 aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg catttcctct
      541 tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa caaattcaac
      601 acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag taattctgga
      661 gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa atattatcgc
      721 aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta catatcataa
      781 tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta aaaatactct
      841 tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa gaagttttga
      901 ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata tacatattcc
      961 ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga atataagaaa
     1021 aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat agtgggatta
     1081 tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta tcaactggct
     1141 tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga catatcaaat
     1201 gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg cctaaattct
     1261 tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt tccaattcaa
     1321 tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc ggcaataaat
     1381 aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta cccccagttg
     1441 gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca caatgcaagt
     1501 ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc taaaattaag
     1561 tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa tcataagcaa
     1621 gagactgtag cggaatattt cggattaata aatgacactg cccatagagc ggaatcagat
     1681 gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga gtcgttggaa
     1741 gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga agcttgtgct
     1801 tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag gtatcgaaaa
     1861 aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt taagtttgct
     1921 aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga tttacctata
     1981 gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt tcgtagcccc
     2041 tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg ttataattct
     2101 atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca aagccttagt
     2161 atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc aaaaaatcgt
     2221 ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag agaagatgta
     2281 attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat agggaattgg
     2341 gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc taggaaaaat
     2401 ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg ctatgatgct
     2461 cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta cgataatgaa
     2521 attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg tactttagag
     2581 gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga gcggatagct
     2641 aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag tgagcctagc
     2701 aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat aaaagaattc
     2761 gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat tagggatgct
     2821 gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga ttagcagata
     2881 atctaagata acctaggatc ttcttaata
//
LOCUS       DBNIBO010000197         2879 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.207, whole genome shotgun sequence.
ACCESSION   DBNIBO010000197 DBNIBO010000000
VERSION     DBNIBO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.207"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            153..2324
                     /locus_tag="AC1Y77_002593"
     CDS             153..2324
                     /locus_tag="AC1Y77_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HIQ1576122.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2392..2733
                     /locus_tag="AC1Y77_002594"
     CDS             2392..2733
                     /locus_tag="AC1Y77_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HIQ1576123.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         1016 a          528 c          648 g          687 t
ORIGIN      
        1 agaaaaaact tacatttttc tgttcattct tatgttactc tacttgcact aacgtaaaaa
       61 aacatattga actacaactt aagcaggaag aaaacactct ttcactatgg taaaataaga
      121 cgattgaatc aaatagacag gagaagaagc aaatggattt tcaaagcatt ttagaaaaga
      181 taaaagctgc tttgatcagt ttttggcgtt ggatcagacc atatctcgga caattccatc
      241 gatggcgaaa gcgcatctgg aaaaaatatc atgtgaacaa attgattttg ctgattggtt
      301 tgatctgtgt cttagtgaca agtatctacc tctttatttt agcaaaacaa gcaaatgtcg
      361 aaacgttaaa atcaggatta agccaatcga cagtcattta tgataagaaa aacgaagaag
      421 ctggcacact gtacgcccaa aaaggaacat atgtagaatt aaacgcgatt tctccctata
      481 tccaagatgc agtaatctcg acagaagata ggaattttta cgagcatcac gggtttgata
      541 tcaaagggat tgcccgtgca gcagtccgaa tggtcttgtc tggcggaaca tcagggggcg
      601 gaggaagtac gatcactcag cagcttgcca aaaatgcgta tctgacatta gaccaaacgt
      661 ttgatcgaaa agcgaaggaa ttatttttgg caatcgagat cgaaaagaag tacagcaaag
      721 aagaaatctt gaccatgtat cttaacaatg cctattttgg caacggggta tggggtgtcc
      781 aagacgcttc cagaaagtat tttggagtag atgcaacgaa tgttacgctg tctgaggctg
      841 ctactttggc aggaatgctg aaaggtcctg gtatttataa tccgatcgat cacccaaaaa
      901 atgcgaatga tcggaaaaac acagtactaa gtgtaatgga agaaaacgga aaaatcacaa
      961 aagaacaaga acaagcagaa agccagacag atatcagcgc gtatcttaat gacacatacg
     1021 ataattcaga ttctggttat cgttatcctt attattttga tgcagtgatc gatgaagcag
     1081 ttaacgagta tggtcttgat gaagaagatg tcatgaataa aggctataaa atctatacag
     1141 ctttagatca ggattatcaa aagcaattag aagctactta tcaaaacagt gcattattcc
     1201 cagcaaatgc ttcagatggt gccatggtcc aatctgcttc agtagcttta gatccgaata
     1261 caggtggtgt acaagcacta gtaggacgaa gaggagatca tgtctttaga ggattcagct
     1321 ttgctacaca gatgcgtcgt tcaccaggat caacgatcaa accattaagc gtatatacac
     1381 cagcactgga agctggatac aagccttcca gtatcttgaa agacgaaccg caaagttatt
     1441 acgatgcgca taattttgac gggacatacc aaggagaagt accgatgtat caagctgttg
     1501 cacaaagttt gaatttgcct actgtatggc ttttgcatga gatcggttta caaaaaggat
     1561 atgataaagc agaagaattc ggcctgcctt tagcaaaatc tgataagtat tatggcttag
     1621 cccttggtgg actggaaaaa ggagtatctc cattgatcat ggcgggtgct tacagtgcat
     1681 ttgccaatga tggtcagatg tatacgccgc atcttatcac gaaaatcgtt gattcgactg
     1741 gagcggtgat cgtcgataac acaaacaaaa aaccaaaaca ggtcatatca aaggaaacag
     1801 ccgatgaaat gaccagcatg cttcttggta cattttcaaa tggaacagcg gctgctgcga
     1861 atcctgcagg ttatacgata gcagggaaaa caggaaccac agaaactaat ttcgatgcca
     1921 caaaagcaaa tgaccaatgg atgatcggtt acacgccaga cgtagtgatt tcgacatgga
     1981 tgggttacga agaatccagc aagctgcact atttggaagg gacaagtgga aatgttgtcg
     2041 ggaaagtctt caaatcagcg gcagaaggca tccttccgta tacttcaaaa acaggattta
     2101 gtgtagcaga tgcctatgcc acaggaggac aagtggtagc tgcagatcaa gttggaaaca
     2161 caggaaattc taatggagag tctggaaatt ggcaagataa cttgaatcaa tatggtgaac
     2221 aagcaaaaga agggctgaaa aacttcggcg atatggtgaa agaaggcgtt cagggaattg
     2281 gtgaagcggc taaagacctc tggcggaaat atcaaggaga gtagcccctt tgaaaacgaa
     2341 taaccccatg ttacaataga gagtaactac taaaagaaaa gagggcataa catgtcaaac
     2401 atctacgata gcgcgaatca aatcgaacgt gagatccgtg aattaccaga attcaaagcg
     2461 ttgaaagaag cgtacgagaa agtacaagca aacgaagaag cacacaaact gttcaaagac
     2521 ttccaagcga tgcaaatcga attacaagaa aaacaaatga gtggacaaga gttcagcgac
     2581 gaagatgcaa tgaaagcaca agaaatggct gtgaaaatcc aatcagaaga agtaatcaat
     2641 gacttgatgc aaaaagagca aggcttcagt acaatcatca atgacttgaa ccgtatcatc
     2701 atgacaccag ttcgcgacct ttacagcgaa taaaactatt ttatgaaaca acagagggtg
     2761 tgaaagagac gttcggcctt gagcgatgac aaaattgctt cttattgccg ataggctgtc
     2821 tcttgaacac catttaccaa gaggtcgtga gcctaccgtt taactctgag ttacaagga
//
LOCUS       DBNIBO010000198         2816 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.208, whole genome shotgun sequence.
ACCESSION   DBNIBO010000198 DBNIBO010000000
VERSION     DBNIBO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.208"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(1..632)
                     /locus_tag="AC1Y77_002595"
                     /pseudo
     CDS             complement(1..632)
                     /locus_tag="AC1Y77_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1117..2751
                     /locus_tag="AC1Y77_002596"
     CDS             1117..2751
                     /locus_tag="AC1Y77_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIQ1576124.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          933 a          416 c          418 g         1049 t
ORIGIN      
        1 gtaaaaaata agtctgattc cttgtctcta aaggcttcgg tcagctcatg gaaaaaagta
       61 taggcgtcct ttaaaggcgt tgaaaatgcc agcaatcgat caatcatcat tgcttcagtg
      121 agtagcgggt atttaggtgc acgaaaactg cgccatgttt tgtaatcata gtgattgatt
      181 ttttgacggt tttttagaag aaagcgccag ttctttttca ctttctcggc ttcccgtcgt
      241 tgccctaact ggtgtaatct tttcatttct tgtacacgaa aatcattgaa cgcctgattc
      301 aaatgtttga tgatatgaaa acaatcaatg acgactttcg catttggtaa gacacgtttt
      361 gtcagttgga agtaggcggc gttcatatct gtcactaaat attccaccgc ttcaggattt
      421 gcacagcgac taaaatagtg tgttaatcga gacaattttc gtgtaggcaa aatatcaatg
      481 agttttccag tctcgccatc cgcacaaata aagctcattt tatccacaat tgaagcgtgc
      541 gaacgaaatt catccaccat caatactttt ggcagggtcc tttttgacgg cttcggaaga
      601 tagtttttca actctttcaa tgtacgaatc atatacctaa aataacaatg ggactgaaga
      661 atcaattttg attcatcagt cccataaatt atagagccta tattttgcga attgcgctac
      721 ggtttcttct tttcttttgt ctgtaatatg aatatataga tccataatga tttgaataga
      781 agagtggcct aaacgctctt gcacatcttt aatatttgca ctagcttgct aaaagcaagc
      841 tagcatgtgt gtgtctaaga ccatgaatag taattcgttt aagattgttt ttcctgatga
      901 ttacatctaa ccattttcga gatttagata attgaaggtg ttcatttctt tctggtaaaa
      961 atttaaatat ataactacat tttctctact aactttgaaa cactactttt gtaatataaa
     1021 aaatatttta ttaataataa aattaatcac attttatata gtgactaatc tagtgtttta
     1081 aaaaaataaa tgatactata tgtaatataa aaaaacatga ggtggtatat gaatttgaaa
     1141 aaaatttttt ctgtaaaaat atttatagtt tatgcatttg ttagtttatt atatttattt
     1201 ccagcattta aaaacatgga aatatttggc ttttatgata cggcatttca cattaatcgt
     1261 gcgttaagtt tagaatctat ttttagctcc cctattaatt ttgaaacgtt tagatcatat
     1321 ggcatgcaag taaataattt ttacccatgg ttaactctat atcctttatt tttattgatc
     1381 aagtttacta atttagcaat aggctataac ctttttcttt atattgttac gctcattact
     1441 ctatttatat gtcattatgt aatgtatgaa attaccaaaa aacatgttac tagctccttt
     1501 ttcgctatta tatatacaac atcttctttt aggagtgtag aaatatttct tcgaggggca
     1561 atgggggaat tactcgcaat gtcaatactt cctcttattt tactcgggtt tataaagctt
     1621 tacgactcaa aaaaagaatc ttgggttatg ttggcgatta gcatgacttt attaatttac
     1681 actcatgttc tttctgtagc tatgactatg gtaatgatta taattgcttt aattattcaa
     1741 ttttatcgga aaaagaaaat agaaatcttc ttaattatta aattaataaa agctgctata
     1801 gttactttac ttctttcact agcgtttata ggtcctatga ttgagcagac attgtatcaa
     1861 gatctgaata gaccttatgt ggatatatta caaaaaagag caatttatct gggtaaagag
     1921 tttttgattg gaagtataga ttcagaatta ctatcatttg gtattggctt aactttgctt
     1981 atttgtttaa ttatactaac atttaacttt aaaaaattag gaatcttatc taaaatcact
     2041 tatataatgg gtataatatc tattctactt tgtacaaaaa tatttccatg gtttgcctta
     2101 caaaatactc ctttaaatat tattcaagct ccttggcgtt ttatgatatt ttcaacattg
     2161 ttcatatctt tttcagtatc tctatttatt tctccacagt tggaaaaatt ttcttataca
     2221 aggagaagaa attttatact attattaata ttagctattt caattttaaa ttattcatca
     2281 gtagttaaca tggtgaaaga aatgaatttt caaggaactt attctaatga acaaatggaa
     2341 aataaacttg aagaatttga gaattttgat tatgtaccta taggctataa aaaagatcaa
     2401 ttgcttttga aaaaccatca agttacagaa gatggtcaag ttatttatag tagcttttct
     2461 ccaaataaag atgaaattac atttacgttt aaatcttcaa aaggcaaaac aatagtttta
     2521 cctgtattcc ggtataaagg attaacggtt actgttaatt caaaaaatgc tacagtacta
     2581 aaacaaggag gtccttttgt tacagtaaaa agtgaaagtg gaataaataa agtaacaatc
     2641 aggtattcat acacattttt ttctaaaatc tcattattta tttctataat cacgctaatt
     2701 gttcttcttt tttattgcat aaaattaaaa ataagaacaa gccatgaatg atttgctagc
     2761 acatatttta gcaatctaaa gatactattt tagatttaat aggccctttg tcaaat
//
LOCUS       DBNIBO010000199         2758 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.209, whole genome shotgun sequence.
ACCESSION   DBNIBO010000199 DBNIBO010000000
VERSION     DBNIBO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.209"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            240..656
                     /locus_tag="AC1Y77_002597"
     CDS             240..656
                     /locus_tag="AC1Y77_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIQ1576125.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            670..1236
                     /locus_tag="AC1Y77_002598"
     CDS             670..1236
                     /locus_tag="AC1Y77_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIQ1576126.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1259..2245
                     /locus_tag="AC1Y77_002599"
     CDS             1259..2245
                     /locus_tag="AC1Y77_002599"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIQ1576127.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2400..>2758)
                     /locus_tag="AC1Y77_002600"
     CDS             complement(2400..>2758)
                     /locus_tag="AC1Y77_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIQ1576128.1"
                     /translation="DSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTT
                     SDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILL
                     VIGLRITFMSYKKSKR"
BASE COUNT          835 a          513 c          574 g          836 t
ORIGIN      
        1 ttgaaaagta attttcgtaa attccttctt atttcttgaa gttgcccact tctgtcacaa
       61 cctcttgtta taaacggtgt tccacctgct gcttctcggt aataagccca aagaattaga
      121 aaacatggaa agctgttttc tattcttcgt tcttattact caaagcaaga cacaggtgtc
      181 acaacctctt gtttttatgc taaaattggg tgagttaatc tggaaaaagg agaacaaaaa
      241 tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt
      301 tgcttttgac tgttctttct tttttaccag tcatgatttt tggtcttcag caaaacaccg
      361 caacagcaga aaaagaagcg gtactgcgtg tggatggcac agaaatcaaa acattccctt
      421 tgatggctgg agaagagagc aacacatata cttacaaaga tgaacacgga gattataact
      481 tgatcgagat agatggcgat cgagtacgga taaaagaagc agattgcgat gaccagatct
      541 gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat
      601 tagtgatcga agtccgttca acagatggag gtgacgaaga cagtctgatt tattagacct
      661 tcacacacca tgagtaaatt acaaaaaatg atttatattt cgctacttgt tgcacaagga
      721 gtgatcattg gtttattgga aaatatgatc ccttatcctt tttcttttgc gcctggtgca
      781 aaactaggct tagccaatct aatcacgatc gttgccattt ttaccatgaa aaaacaagac
      841 agcttcttac tactttggct acgactattc ctgactactt tgctaggcgg aacgatttcc
      901 acattccttt atagtatgag tggtgctttg cttagttatt ttggaatgtt attggtcaag
      961 caattaggac caaaacgtgt cagtatcatc ggaattagtg cggccggcgg tttcatgcat
     1021 aatgtcggac agcttttgat ggcttctttc attgcacaat catggacagt catgttgtac
     1081 ttgccagtcc tttcagtttt agggatttta tcagggattg caatcgggat cgcagcgaat
     1141 tatcttttac gacatgtgca gacacttcaa cgattccaaa aagattatga gcagggaacc
     1201 aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt cacacaagga gtttttttat
     1261 gaagctacat ccaatgtggg aaaactatcc tgctttaagt aaagaattga cagaaacact
     1321 aaaacggatg gaagaaactg tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga
     1381 aacgatccat gccggcggaa agctgttgcg tcctgcctat cagctattgt tctcacaatt
     1441 tggaccagag caagatcgag acaaagccat cgctttggca gcttctatcg aaatgctcca
     1501 tacggccaca ctgatccatg atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc
     1561 taatatccgt agtcagtttg gtaatgtcgt ttctgtctat gcaggagact atttatttgt
     1621 ctgctgtttt aaactattgt ctgattactc cacttccttg aaaagtctcc aattgaattc
     1681 tcgcagtatg gaaaaagtat tgaatggcga attagggcag atggatgatc gctataatta
     1741 tgaactttct gtaaaagaat atttggaaaa tatttcaggt aagacagctg aactgttcca
     1801 attaagttgt tctgttggcg cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc
     1861 aggagatatc ggactttcta tcgggatggc ctttcagatt atcgatgata ttttagatta
     1921 tacaaaagag agccaggaaa tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta
     1981 ttctttaccg ttgatctact ctttgcaaaa aaacaaacca aagctactac cttatttaga
     2041 gaagaaagcg gcaatgacag aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac
     2101 aggaggcgtg gaagaagcta ggaagttagc ggcctcttac acagaaaagg ctttgaagga
     2161 aataaagaaa ttacctgcca cctctcttcg aacaaaagag aatctctttt cccttaccca
     2221 actgattttg gatcgcaaag actgaatagg gaaaggttcg atacgtaaat aaatttctca
     2281 gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt tcttgtattt atgataaaaa
     2341 gcaaaaataa agagcaacgt ccgttagcga cgttgctctt ttttatcttg aatatttttt
     2401 taccttttcg atttcttata actcataaaa gtgattctta aaccgattac aagtaatata
     2461 acgcctgcga gtgttatcca attattcgat tgacttccag ttttaggaag tgctcttttt
     2521 gatttgtttc cattgtcatt gcttgtttta ttagttaagt taaagttcgt cctgttatcc
     2581 gaacttgttt ttgagaagga gacacttgca tgacgtgtat cgcttgtggt agacgacgtg
     2641 tctgtactat cgcttgaagt tgatgaatct gagctatcac ttgtggtaga ggatgtatct
     2701 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg tagtggtaga tgaatctg
//
LOCUS       DBNIBO010000200         2745 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.210, whole genome shotgun sequence.
ACCESSION   DBNIBO010000200 DBNIBO010000000
VERSION     DBNIBO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.210"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            1..151
                     /locus_tag="AC1Y77_002601"
                     /pseudo
     CDS             1..151
                     /locus_tag="AC1Y77_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            462..959
                     /locus_tag="AC1Y77_002602"
     CDS             462..959
                     /locus_tag="AC1Y77_002602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576129.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            960..1376
                     /locus_tag="AC1Y77_002603"
     CDS             960..1376
                     /locus_tag="AC1Y77_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HIQ1576130.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1378..>2745
                     /locus_tag="AC1Y77_002604"
     CDS             1378..>2745
                     /locus_tag="AC1Y77_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576131.1"
                     /translation="MTEKNIMVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNS
                     SYEVQVSHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVIT
                     KSISRFARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQS
                     LSQNVKLGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQS
                     LVGIGRALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKN
                     EGHVPQYYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFC
                     GDCGDIYRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLA
                     GGNNMIKTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDE
                     LRDKRQ"
BASE COUNT          988 a          480 c          583 g          694 t
ORIGIN      
        1 cggcatgaac gcaagccctt tatatacctt gaatttacaa caaaaaggcg tacaaggtac
       61 attttcatta ggacgtgtac aaacaccgac tttatatctc atttatcaac gccaggaagc
      121 aatagaaaac tttaaaaaag aacctttttt cctgaataat tcatagtttt actaatagtc
      181 tttcctaaca ttgatttaat agtctttaaa gtattctgct tcatcaccca ttaggtgatg
      241 aaaaaagaac ccctttctga tttagcggac aaatactttg acctaatctc agaagatata
      301 aaagaaaaaa taataaaata tccgatttat actttgtata aagaactaaa gtagttagca
      361 aatcatttac agcaaatcac tatgttccac ataccatttt acatacttac cttatccacg
      421 acacatgttg agatagtatc gaagataaag aaagttgctc catgttgttt ttgcgagatg
      481 gggaaatcaa agccacattg acaaccatga tgaataagct tgctttcagt cataagctaa
      541 tcctagaacc acttttcaag tcagttagcc aaatcgatga agaaagcgac cgtgaaagaa
      601 tggacgctat tgataagcta atggagcaac tattggaaga acgcaatacc cttattgcac
      661 tgatgtccaa aggtttccta gaacctgctc tttttaatca ggaacgaaat gttttagata
      721 gtgagattaa aaatcttact accgaaaaaa caaacctggt aacgaactct gcgagtgggg
      781 ttttgcgagc aaacgagata aaggacctca ttaattatgt gtcagcagat aattttaatg
      841 gtgactacac ggaagaacta tttgaagaat ttgtagtgaa catcattgta aattccaggg
      901 atgagctgac attcaatttg aaatgcggtc tttccctgaa agaaaaggtg gtgagataaa
      961 tggcatatat tccatatgga tacaaaattc aagatggagt ggttactgtc gatgaaaagg
     1021 cagcaggtca agtaaaggta ttctttgaga aatacatatc aggactatcc cttacagtgg
     1081 ctggcgaaca ggcaggtatt gaaaagacac attctgtgat ggggcgcatt ttgaaaaacg
     1141 tcaactacct tggaaatgat acgtatccag caattattga taaagagata tttgataaag
     1201 ctgaagaagt tagagataaa cgtgcaaagg atttaggacg agtggtagag cttgccgctt
     1261 tcacctctcc ccctcccaaa gaacgattca aaatgaaaaa ggcagataat aagatgccag
     1321 tggatccttt tgaacgagca gaatacttat acagtctgat agaaagcgag gaataaagtg
     1381 acagagaaaa atataatggt tattcctgct cgtaaaagag taggaagtac agccgcaaaa
     1441 gaaaagataa agaaacttcg tgttgctgcc tattgccgtg tttctacaga aacagaagaa
     1501 caaaattcta gctatgaggt tcaggtctca cactatacag agtttataaa gaaaaataat
     1561 gaatgggagt ttgctggcat ctttgcagat gatggtatct ctggtacgaa cactaaaaag
     1621 cgtgacgaat ttaatcgcat gattgcagag tgtatggacg gtaacatcga catggttatt
     1681 acaaaatcca tcagccgatt tgcgcgtaac accctagact gccttcaata cattagacag
     1741 ctcaaggata agaacatatc agtttatttt gagaaagaga acatcaacac cacggatgcc
     1801 aaaggtgagg ttttgctgac tattatggca tctttggcac agcaggaaag ccagagcctt
     1861 tcacaaaacg ttaagcttgg gctacagtac cgataccaac aaggaaaggt gcaggtcaac
     1921 cataagcgat ttatgggcta cacgaaagat gaagatggaa atttaactat tgtccccgaa
     1981 gaagctgaga ttatcaaacg catctaccga gaataccttg agggtcagag tctagtgggc
     2041 attggtcgag cccttgaaaa ggatggtatt ttaacagcag cgggaaaacc aagatggcga
     2101 ccagaatcag ttaagaagat acttcaaaac gaaaaataca tcggagatgc ccttctgcaa
     2161 aagactgtca cagtagattt tctgaccaag aaacgagtga aaaatgaagg gcatgttccc
     2221 caatattatg ttgaaaatag ccatgaagcg attattccta aagagttatt tttgcaggcg
     2281 caagaagaaa ttcatcggag aagcaatatc tacacaggag aaggcaagaa caaaagaatt
     2341 tacagtagca agtacgcttt aagtgccatc accttctgtg gagattgtgg cgatatttat
     2401 agacgggtct attggaatat ccacggaaga aaagaatttg tctggcgatg cgtgactaga
     2461 atcgagcaag gtcctgaagt ttgtaagaac cgaaccgtaa aagaagatga actttatggt
     2521 gcagtaatga ctgcgattaa taagctactt gcaggtggga acaatatgat aaagacactg
     2581 gaagagaata ttcatgctgt gattggcgaa accacagaat accaaatttc agagattaac
     2641 aactcactag aggaaaaaca aaaagaacta atcaagctgg cgaacaaggg tcaagactat
     2701 gaccacttag cagatgagat tgatgagctg agagacaagc gacag
//
LOCUS       DBNIBO010000201         2583 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.211, whole genome shotgun sequence.
ACCESSION   DBNIBO010000201 DBNIBO010000000
VERSION     DBNIBO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.211"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..665
                     /locus_tag="AC1Y77_002605"
     CDS             <1..665
                     /locus_tag="AC1Y77_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576132.1"
                     /translation="AYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNL
                     TMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGF
                     ILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVT
                     VKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKV
                     VI"
     gene            709..978
                     /locus_tag="AC1Y77_002606"
     CDS             709..978
                     /locus_tag="AC1Y77_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576133.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1210..1506
                     /locus_tag="AC1Y77_002607"
     CDS             1210..1506
                     /locus_tag="AC1Y77_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576134.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1508..1930
                     /locus_tag="AC1Y77_002608"
     CDS             1508..1930
                     /locus_tag="AC1Y77_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576135.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1949..>2583
                     /gene="mobL"
                     /locus_tag="AC1Y77_002609"
     CDS             1949..>2583
                     /gene="mobL"
                     /locus_tag="AC1Y77_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HIQ1576136.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEG"
BASE COUNT          990 a          362 c          492 g          739 t
ORIGIN      
        1 tggcctatta cttgttaagt gagccaaaac agatcgcggc gtcaaatatt ttcacggcag
       61 taatgacatt aatgtgtgtc atgtgctttt ctatttttag ttacttactc gtccaattta
      121 tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt attggactta cttatcttag
      181 gcagtatgtt aacgactcta acagatcggt tcgaacaatc tgaaaaaacg gtagagggga
      241 taaagaactt cttaacgacg attgatcaat ttataaatgg atatatgtat ttgtttgaag
      301 taggctttat tctcactttg tttggttgtg tatttataaa actcattaaa agatcaatcg
      361 aaaaagaaat ggttattgat ctaattccga cgctgagcgg ctatgagtat tacgttgatt
      421 ttggaaagta tgaggagttg atccatacgg atatcgttca cacgagtatc catgaagtaa
      481 ccgttaaggg catcagatct actttaaaag atggacagga gacgaaaaca atcgaccaaa
      541 gaaaagaggt ctacaatttt tataaagttg aagcaagtct acgatatggc attgggaaat
      601 tgaggttaga tgggagtaat attcaacaaa acaaaccata tgttttagga aaagtggtga
      661 tttagaactt cctatgaaag atgaataaat aaggagaatc ttttgaaaat gattcttctt
      721 tacagaattg tgcttatagt tggcacggag ttaaaaatct taaagaagga gcagttattc
      781 aaagtgaaag aacatcataa tcagttacta tgtattaaaa cgggtacgat gaacattcaa
      841 taccgaacag aatatttaga attaacgaat caaactgatt ttttgtcgat tcgagagcaa
      901 cataagcaag aaaaattaat ggctacttat agccaattga ttgatgagtg ggaacaattc
      961 aaggaatcta aaacataaaa ttccatttaa acaccttatt ttttaaagag cataaggaaa
     1021 cgtgacccca cttttaaaaa aagtggttgg ataggctgtg cctatcacgt ttctgaaaaa
     1081 aataaatgac cccaaaatac caatatatag gatagtacaa ggatagcaaa ggatagtaaa
     1141 aggatagagg taggatagcg aaaaggatag gatccttttc actatcctat attatattgg
     1201 aggatattta tgaatgttaa aattagagat ttagatccaa aattcatttc tgaaattgat
     1261 cgaagatgcg ttgaactaag taatcgtaca ggaaataaat ggagtcgtaa tgattattta
     1321 aagctactcg ttgaaaatga ttttgatcgt cctttgatgg aatacaaaca agaaaagttt
     1381 gaccagttat tagaaaagtt ttcagaaatt caaagttata acacaaagat attagaagaa
     1441 tatgttagcc aaaataatcg tattatagaa atcttgattg agcaaaatag aggaagtgaa
     1501 ctttgatatg gcaaaggtta tagacaaaca catttacttt caccaaacac atatcgatgt
     1561 attggatgaa actatttcta ctcatgcaga ggttaaaaac tatagtgaag ctgttcgctt
     1621 tttatgtatg aactatgatc cagaagtgga gaaaaaagta aattccaatc aagcaaagtt
     1681 gaatggaatg agtaaagaaa tttctatttt aaccgaaatg gtttcagaat ttgcggatct
     1741 tcatttgaaa gatacaggaa tattaattgg taaaaattca actgtttatc aagaagcaaa
     1801 agaacgagtg gaaggaaaaa ttaaatccaa tcaaacaaaa aaatattccc ctcagttgaa
     1861 gaaacctgtg aatgaatctg ttcatgccag agtagatcca tttgatccaa cgaagattct
     1921 aaaaagataa aaatgaagga ggataataat gactcaatcg ccttcagtta ttctcacttg
     1981 tcaatttact ttgccgaata aaaagtcatt ttctacttat attgactata ttacaaggga
     2041 acaggctctt gaagaaaagg aacgacgtac gcccgaagaa gaacaggaac ttcaacgaat
     2101 caagaatgct ttagaggagt tttatattcc tgaaggagaa actttttcgg agagtaagac
     2161 gggtaaagaa ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa
     2221 ttttgacaag ttagaggaac aagattttac caattatctt tcttatatga cacgtcacta
     2281 tgcgttagaa caaaagaaag aactgacgca aagtgaacaa aaagaatacc aacgcttgaa
     2341 ccaagcaatg aaaaaatatc aaacagaacc ccataaaaaa gcttcaggaa atcttcctgg
     2401 agttttttct atagcttcag acgaagtgaa aggaagtgat ctgaaggaga ttaagcaaat
     2461 cttccaaaga ggacaacaaa aaggctcaat catttatcaa gatgttgttt ctcatgataa
     2521 ttcgtatctt gaaaaaatag gattgtacga tcccaaaaca gatactttag atgaagaagg
     2581 gtt
//
LOCUS       DBNIBO010000202         2439 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.213, whole genome shotgun sequence.
ACCESSION   DBNIBO010000202 DBNIBO010000000
VERSION     DBNIBO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.213"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..382
                     /locus_tag="AC1Y77_002610"
     CDS             <1..382
                     /locus_tag="AC1Y77_002610"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576137.1"
                     /translation="RITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDNPYSQFLI
                     TVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMT
                     VNQICEITNVSRASLYRKLSEVNN"
     gene            596..1291
                     /gene="vanR-A"
                     /locus_tag="AC1Y77_002611"
     CDS             596..1291
                     /gene="vanR-A"
                     /locus_tag="AC1Y77_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HIQ1576138.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1269..2423
                     /locus_tag="AC1Y77_002612"
     CDS             1269..2423
                     /locus_tag="AC1Y77_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HIQ1576139.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          840 a          437 c          515 g          647 t
ORIGIN      
        1 tcgaatcact cgtagtacac aagatctatt tgaattaatc gataacatac gagataaaaa
       61 ggcaagttta aaatcactaa aagatacatg gcttgattta tcagaagata atccatacag
      121 ccaattctta attactgtaa tggctggtgt taaccaatta gagcgagatc ttattcggat
      181 gagacaacgt gaagggattg aattggctaa gaaagaagga aagtttaaag gtcgattaaa
      241 gaagtatcat aaaaatcacg caggaatgaa ttatgcggta aagctatata aagaaggaaa
      301 tatgactgta aatcaaattt gtgaaattac taatgtatct agggcttcat tatacaggaa
      361 attatcagaa gtgaataatt agccattctg tattccgcta atgggcaata tttttaaaga
      421 agaaaaggaa actataaaat attaacagcc tcctagcgat gccgaaaagc cctttgataa
      481 aaaaagaatc atcatcttaa gaaattctta gtcatttatt atgtaaatgc ttataaattc
      541 ggccctataa tctgataaat tattaagggc aaacttatgt gaaagggtga taactatgag
      601 cgataaaata cttattgtgg atgatgaaca tgaaattgcc gatttggttg aattatactt
      661 aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat tggaatgtat
      721 agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca caagcggcct
      781 tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc tgaccgggaa
      841 agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt atataacgaa
      901 gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc gatacaaaaa
      961 attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc ttgtcattaa
     1021 tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc ccaccgagtt
     1081 ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg agctgctatt
     1141 tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca ccgtgcatat
     1201 ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata taaaaacggt
     1261 atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa ctagaacgaa
     1321 aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg ttgtatattc
     1381 gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa aacaaatatg
     1441 acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac aatatagata
     1501 tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc atgctttcaa
     1561 aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt cagaacgaag
     1621 ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc aacacattaa
     1681 aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga aaaaatgacg
     1741 ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc ggttatttga
     1801 gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat gtgcatatca
     1861 cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag attacacggt
     1921 ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat atgctggtgc
     1981 agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg gttattcacg
     2041 cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc tttaacaaca
     2101 ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt accgcgggcc
     2161 tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca aaagataagc
     2221 tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc gatacgggtg
     2281 gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga gggcagattt
     2341 acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg atgccagact
     2401 tggttgataa aaggaggtcc taagagatgt atataattt
//
LOCUS       DBNIBO010000203         2424 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.214, whole genome shotgun sequence.
ACCESSION   DBNIBO010000203 DBNIBO010000000
VERSION     DBNIBO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.214"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            344..1924
                     /locus_tag="AC1Y77_002613"
     CDS             344..1924
                     /locus_tag="AC1Y77_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIQ1576140.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2122..2346
                     /locus_tag="AC1Y77_002614"
     CDS             2122..2346
                     /locus_tag="AC1Y77_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HIQ1576141.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          874 a          389 c          511 g          650 t
ORIGIN      
        1 ttttagtcag ctgacaaaaa aatatttgac tcttatggtt tggtggcttt ttcattcgct
       61 gaatccatgc tatactgatt cagttgtagt agttgacaaa ggttgtgaaa aagctatcct
      121 gcattaagta ataagagcag ccaagcaaaa acagcttttc gtatttttgg cttgggtggg
      181 cttattgctg ataggctgtc tctagaacgc cgtttactcg gaaaaagaga ctgctacacg
      241 gcttttgtca cagccctata tgaaattgtc tttctttaag agatcggaca atctgtgcct
      301 agagaaaata taaataattg aatggaagaa atggagttag gttatgaata atccaaaatt
      361 aaagcaacaa gtggacaatc gcagaacttt tgcgatcatt tcccatccgg atgctgggaa
      421 aacaacgatt acagaacaat tactattgtt tggtggggct atccgtcaag ccgggaccgt
      481 aaaaggcaaa aaaacaggta attttgccaa atcagactgg atggaaatcg agaaacaacg
      541 tgggatttct gttactagtt ctgttatgca gtttgattat caagggaaac gagtaaatat
      601 cttggataca ccaggacatg aagatttttc tgaagatact tatcgtactt tgatggccgt
      661 ggatagtgcg gtcatggtaa tcgatagtgc aaaagggatc gaagcacaga caaagaaatt
      721 attccaagtt gtaaaaaaac gtggcatccc gatatttact tttatcaaca aactagatcg
      781 tgatggtcgt gaaccattgg aattgttaga agaattagaa gagctattag atatcgaatc
      841 ttatccgatg aattggccaa tcggtatggg taaagggcta gaaggtcttt atgatatcta
      901 ccataatcgt gtagagtttt atcgtccgga aaattatcag gatgaacgat tagtagaatt
      961 ggatgaagat ggactgcttc ccgaaaatca tcctctaact gaaaattcct tatatgaaca
     1021 agtcttagaa gaagtagaac taatcaaaga ggctggagat gcatttaatc aagaaaaaat
     1081 tgctcgtggc gatcagacac cagtattttt tggttctgca ttgactaact tcggtgtaca
     1141 gactttttta gagactttcg ttgaattggc accagctcct tatggacaca aaacacaaga
     1201 tgatcagatg gtaagtcctt atgaggaaga attttcaggt ttcgtattca agatccaagc
     1261 aaatatgaat ccagcccatc gtgaccgaat cgcttttgtc cgaatctgtt ctggaacatt
     1321 cgaacgagga atggatgtct ggttggaacg tacaaataaa aaactgaaat taagcaatgt
     1381 cacacaattt atggcagatt cgagagaaaa cgtcgaaaaa gcagtagcag gcgatatcat
     1441 cggtgtatat gatacaggca attatcagat tggggataca ttgttcgaag ggaaactaaa
     1501 agttgcttat gaagaactcc cttcatttac tccagagctg tttatgaaag tgactgcaaa
     1561 aaatgtcatg aaacaaaaat ccttccacaa aggaatctat caattagttc aagaaggagc
     1621 gattcagctt tataaaacat atctaacgga agaatatatc attggagctg ttggtcagct
     1681 acaattcgaa gtattccaat atcgtatgtt aaatgagtat aacgccgaag taatcatgtc
     1741 tccaatggga cacaaaatcg ctcgctggat cgatccagaa gatttggatg agaagatgag
     1801 ttcaagtcgc aatatcttgg ctcgtgaccg tttcgaccaa ccgttgttct tattcgagaa
     1861 ccaatttgca gaacgctggt ttgcggataa atatcccaat gtaaaattaa aaagtttgat
     1921 gtaaagaagt cttgacacaa gctgccctgc atcaagtaat aagaatagcc aaacaaaaac
     1981 agcctctcat attttgttta gagccgtgac ggattttccg tgacggctct ttttactata
     2041 ttaaaatcat agaaatgtct aatcaaaaaa atataaaaat tattttgcgt acatatgatt
     2101 aaagaaagga gagtgtgaca catggaaaca gaatggcaaa gacgtgaaag aaaaaaacag
     2161 aaaaaagaag agatgttacg attatgtcga ttattagatg taaaagactg ggacaaaaac
     2221 gagcaaattg cacaaaaagt acgatcaatc atccataaag aggaaaaagt taaaaataaa
     2281 gacacgcgta gaactgaaaa tgaaaagaga aaagtacaaa agaaaagaga aaaatcaaaa
     2341 agctagaaag aaaaagaggc tgagacagaa gcgtttaact ccaagaaata agctggaatt
     2401 cacaaaaatt tgaaaagcaa tttt
//
LOCUS       DBNIBO010000204         2419 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.215, whole genome shotgun sequence.
ACCESSION   DBNIBO010000204 DBNIBO010000000
VERSION     DBNIBO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.215"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            5..1150
                     /locus_tag="AC1Y77_002615"
     CDS             5..1150
                     /locus_tag="AC1Y77_002615"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIQ1576142.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="AC1Y77_002616"
     CDS             1321..1779
                     /locus_tag="AC1Y77_002616"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIQ1576143.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="AC1Y77_002617"
     CDS             1938..2408
                     /locus_tag="AC1Y77_002617"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIQ1576144.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          700 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DBNIBO010000205         2324 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.216, whole genome shotgun sequence.
ACCESSION   DBNIBO010000205 DBNIBO010000000
VERSION     DBNIBO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.216"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="AC1Y77_002618"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="AC1Y77_002618"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIQ1576145.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="AC1Y77_002619"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="AC1Y77_002619"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIQ1576146.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT          841 a          371 c          490 g          622 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttt
//
LOCUS       DBNIBO010000206         2309 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.217, whole genome shotgun sequence.
ACCESSION   DBNIBO010000206 DBNIBO010000000
VERSION     DBNIBO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.217"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..1218)
                     /locus_tag="AC1Y77_002620"
     CDS             complement(<1..1218)
                     /locus_tag="AC1Y77_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576147.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKIL"
     gene            complement(1999..>2309)
                     /locus_tag="AC1Y77_002621"
     CDS             complement(1999..>2309)
                     /locus_tag="AC1Y77_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF7006 family protein"
                     /protein_id="HIQ1576148.1"
                     /translation="KNFFEKKGDYDAYNYVLNLEKQTYYTLRKNDDNDFWTMLPKMLS
                     IDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGYKVDEQCPQSIIFYIE
                     "
BASE COUNT          812 a          348 c          260 g          889 t
ORIGIN      
        1 taaaatctta atcggtttaa catttgttaa aaataaatca taataatcta acacataata
       61 ataatccaaa aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt
      121 tgcttgtaat tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt
      181 catttcttcg atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact
      241 tctttcatga aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa
      301 acggaacatg ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt
      361 ttgtcgctga tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc
      421 aaaagaataa attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat
      481 ttgttttgac cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata
      541 ttcattatct actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa
      601 agaaaataca cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac
      661 tatatacttc tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg
      721 tgtagaattc caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa
      781 ttctaactga aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt
      841 cgcatatctg aaaacagtag atacactagt aaattgctct ttagcaaaat cttttagaac
      901 acttctagga tatttataca taaaatcaat caaatgataa ttataagaag ctttataaaa
      961 atgaagataa atattataaa taggaaaatc tttctctttt tttatgaaaa atcgtttatt
     1021 atcttcattt ttcagaagga tattaccatt atattgctta aataaatcct ctagttcttc
     1081 tatatagtgt aagactgact tctccgtaac attaagtaaa tttctaagtt cttccattgc
     1141 acaccaattg tctttttcta acaaataatc taataaaact acttttttga aagatgtttt
     1201 agttaaaaaa gtttgcatca tttcacctca ataagttact tttgtttatt ttaatcaaag
     1261 aataaacttc tctcgttcga ctataactga tcgaacaaaa gaagcctttt aataaatata
     1321 atctcactta catacatagg ctagttattt gcttctagct tatgataagt atataaacgg
     1381 atatcgattt cataattgtt ttatcttaaa catcatagct gtaattgttt ttatttttac
     1441 atgaagaatt attataatct agtttaattt ttttctgaaa tatagaataa aaataaaaac
     1501 atcttttcat tttgagttat taatggagat gtaaatgatt aacaatatgt aatttaattt
     1561 tttattcctg cattaagata atttgattgg taaataaaaa caaacgtaaa ttcgttattt
     1621 taattcctat ttattgtgaa catatccaat ttttatcaca tagatatttg tataaacatt
     1681 ttatgaatgc gcttgtattt aatttagaaa gtgttactat ctatttaggc aaatttttgc
     1741 taatattttg ttttttcatt taccatctgt agaaatcatt taatgatttc tatcttctcc
     1801 ttccaacttt tctgatgatg catcatgaat taacagtgca ttatccatct tttccatgtt
     1861 aaaagaacta agaatcaaga taacgtcttg attcttagtt cttttattcc ttctagaaaa
     1921 taattgttag caatatagtt tatatttgta aatactaaat cagtagcttc tagttgcttt
     1981 cctgcttaat agttcatatt actcaatgta aaaaataatt gattgtggac attgctcatc
     2041 cactttgtat ccacaacttt ctttataata ggtatgataa tcactctcta ccatattaat
     2101 taactctttt cccccgcaaa attcttcaat gtctaaatta ataaattcct ctaaaaggga
     2161 aagcttagag tcaatcgaaa gcattttagg taacatagtc caaaagtcat tatcatcgtt
     2221 tttacgtagt gtataatatg tttgcttttc tagatttaat acataattat atgcatcata
     2281 atctcccttt ttttcaaaaa aatttttca
//
LOCUS       DBNIBO010000207         2259 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.218, whole genome shotgun sequence.
ACCESSION   DBNIBO010000207 DBNIBO010000000
VERSION     DBNIBO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.218"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(148..>2259)
                     /locus_tag="AC1Y77_002622"
     CDS             complement(148..>2259)
                     /locus_tag="AC1Y77_002622"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIQ1576149.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DBNIBO010000208         2241 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.219, whole genome shotgun sequence.
ACCESSION   DBNIBO010000208 DBNIBO010000000
VERSION     DBNIBO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.219"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(1..347)
                     /locus_tag="AC1Y77_002623"
                     /pseudo
     CDS             complement(1..347)
                     /locus_tag="AC1Y77_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            556..930
                     /locus_tag="AC1Y77_002624"
     CDS             556..930
                     /locus_tag="AC1Y77_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HIQ1576150.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(1044..1673)
                     /locus_tag="AC1Y77_002625"
     CDS             complement(1044..1673)
                     /locus_tag="AC1Y77_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIQ1576151.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1807..1879)
                     /locus_tag="AC1Y77_002626"
     tRNA            complement(1807..1879)
                     /locus_tag="AC1Y77_002626"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Thr,seq:ggt)
     gene            2093..2166
                     /locus_tag="AC1Y77_002627"
     tRNA            2093..2166
                     /locus_tag="AC1Y77_002627"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2127..2129,aa:Asn,seq:gtt)
BASE COUNT          731 a          348 c          382 g          780 t
ORIGIN      
        1 tagacctaat attctgcaca tcgctgatat agggtaaaga tgcttattcg catcgattac
       61 ttgtctcttc gtccgaatat cagcgctgct tgctttaaaa tatcattttc catttctaat
      121 tgctggtttc ttttacgtag ttctaacaat tctttttgtt caggcgtaag attatctttt
      181 tctttgaatg aaccactcgt tttagattgc tttacccatt tgtcaaatgc tgaagccgtt
      241 agttcatatt ctcgaatgat ttctacacgt ggctttccag ctaagtaaag attgacgatt
      301 tgttgcttga attcttgtga aaaagttctt cgtgttctct tagacataaa aattcctcct
      361 ggtatgtttt cttctagtct acacacctta atttttctgt ctagttaatt gtagcctatc
      421 caagtctaca ctctgagatg taagaaatat cataagatat ttcttacatc tttttgtttt
      481 gagcaggact ttagataatt ttattaatat atgctagcat gcttttgtaa gaaaaagaaa
      541 gagggcgtat tagttatgta tcttaaaatt acggaacaag cacaagaaag attacaaaaa
      601 tatattgatg aaggcgcaac agttatattg gatttagatg atggcgttgg ggaatattct
      661 aaaatgggtg tttgctcttt agatactagt tttcgtttac ttcttcttga taaagaacag
      721 agaaaaaatg attataaact ccaattagat agtgatattg gggatgttta tataaaagat
      781 tattcaaaaa tgtacatgga tgaacatatg accctttctt tagatccacg tctaggtgta
      841 tttactcttg aaagcccaag tggttcttta gacagtcatg ttcaattagt agatctacga
      901 ccagaaaaat tctctggaat aaaacaataa ctttctctct attgtccata aaactagtct
      961 caatcaagct gtattgaaaa aacaagacaa cattcttacc atggtaaata aaaagacgac
     1021 acattttgtc gtcttttttt aaattaacta ttttcgataa aagatcgcat agcttgtgcc
     1081 attatttctg gcttttctct attcaattca tgttttccgt ttttaataaa gagtagccgg
     1141 ccattcgttg aaagtttata taaacgtttt gctgctggtt tattcgcatg atctttggta
     1201 ccgcataaaa tcaatttagg aagtcgtgat gtacgaagct gttttttgca gtctaatgtt
     1261 tcaagtgaac gaagtagggt aagggtttgt tttttagata gacctaattt ttcaaatatc
     1321 ttagaaggta cgcagtaaaa ataaatattc tggaaagtaa gcaataagcg attaggagac
     1381 tcaaattgtg gagcgactaa aacaaccttt tttaaatatg gagaaggagt tgcaagatag
     1441 tttaaagcaa taacagcgcc taaagataat ccacataaaa taaaaggttc ctttatctga
     1501 ttcaattgta cttcgatgta tttggttaag tcgtctaaag tagcagtgtc tgtgctgttt
     1561 aaatgattga acaatgaaag acttttgcaa gaataactat tgggtagata agagattaca
     1621 tctttccatg attgttcgtc ttggcctaga ccatgcaaaa aaacaatttt catattgctc
     1681 tcctttttat agttatcata tagactttct ttgtactatt aaaatataaa cattcatttc
     1741 ttacgtcata aaataaaaaa acagcgcaga cacattgtta aaccaacatg ttgcactgtt
     1801 taaggaagcc acttgccggg cttgaaccgg cgacctcttc cttaccatgg aagcgctcta
     1861 ccagctgagc taaagcggca ttatcattaa tacaaataat aggataacga atattaatgt
     1921 atttgtcaac agaatttttt cgaaaaaatg tgtttctcta ataggaaaac agattttaga
     1981 aaaaataaaa ataacaaaaa atgcgaacgg tttctttgat aatataataa aaaaattcaa
     2041 aaaaagtatt gcgttattta gaagaaattg atattatatt catgtacttg attccggcat
     2101 agctcagttg gtagtagcgc atgactgtta atcatgatgt cgtaggttcg agtcctactg
     2161 ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt ttctttttct
     2221 tttattatta tgatgaatca a
//
LOCUS       DBNIBO010000209         2202 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.220, whole genome shotgun sequence.
ACCESSION   DBNIBO010000209 DBNIBO010000000
VERSION     DBNIBO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.220"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..244)
                     /locus_tag="AC1Y77_002628"
     CDS             complement(<1..244)
                     /locus_tag="AC1Y77_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIQ1576152.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            482..1336
                     /locus_tag="AC1Y77_002629"
                     /pseudo
     CDS             482..1336
                     /locus_tag="AC1Y77_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1418..1612
                     /locus_tag="AC1Y77_002630"
     CDS             1418..1612
                     /locus_tag="AC1Y77_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576153.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            1602..1955
                     /gene="tnpB"
                     /locus_tag="AC1Y77_002631"
     CDS             1602..1955
                     /gene="tnpB"
                     /locus_tag="AC1Y77_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HIQ1576154.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          765 a          372 c          401 g          664 t
ORIGIN      
        1 cttgccatac ctcctgaatt tgaccatcag gagtcgtaat gaaaatagta tcattttcat
       61 ctactaattc tcttaagttt aaatattgtt tactagatgt aaaattcgaa gctgctaatg
      121 acgagtcttc gcttttacta taaaatcctg ttacttgtat tttatgttcc tggaaatact
      181 tcccgaatga acaacctacc ttgccggctc caataaaacc aacgttcata attatcacct
      241 ccataaagaa gtgagactac aagtcccatt cttacgatat gagcttaaaa ataaaaatta
      301 aatcaaatat acagtatgtt tttttcaaat atcatccata gttaatttat cataatatcc
      361 catattttaa aataaaagat agcgtaaaat attttgtgta aatagaaatt tagggtagaa
      421 aaaaaagtcc attctgtaaa attaaagtta ccacacagaa attttatagg aggactttct
      481 catgactaaa tttactatag aaattatgca aacactactc aataaagggg atttagatga
      541 attatttcag gatcatttag agcgagcgat taattcactt ctacaagcag agctaactgc
      601 ttttttagat tacgaaaaat atgatcgaaa tggattcaat tcaggaaatt cccgtaacgg
      661 gaattattca cgtacattta aaacagaata tggcgagttg aatttaacta ttcctagaga
      721 tcggaatggc gaattttctc aacaaacact accagcatat aagcgaacca atgattcatt
      781 agaaacgacc attatccaat tatttcaaaa aggcattact atggctgaaa tctccaaatt
      841 aattgaaaaa atgtatggtc accactatac gccacaaaca atctccaaca tgagtaaatt
      901 ggtggctgaa gatgttttag cttttaaaga aagaacgtta gaagccaatt attccgttat
      961 atttatggat gcgacgcata tccctgtgaa gcgacagact gtttcgaagg aagcagtgta
     1021 tattacgatt ggtattcgtt tggatggaac caaagaagtt cttgggttta ctattgcccc
     1081 aactgagtct gcttatattt ggaaagaagt tcttcaagat cttagaaaac gtgggttaga
     1141 agaagtttta ttagtagtga cagacggatt aagctgtatt gaagaaagta tccatagtgt
     1201 gtatccgaat gcccaatttc aacaatgttg tgtgcatgta tctagaaata tcgctcataa
     1261 agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc aaattggttt accaagcgaa
     1321 ttcaaaagaa gaggcgtaag cgccccatag aacagtacct tactaacaaa tgcacctcaa
     1381 gatatacttg agctatcaag aagtctggag gttttctatg tataaattaa atgagattgt
     1441 tcctgtccaa ttaggaaacg aaaagagtgc accagaaaaa ggtaaacccg cggctgccaa
     1501 gttagcgatg cgcatcaaaa gagagaatgg ttctgaagtt ttgatctata acggtattaa
     1561 caattatatt cttcaagcta ttttgaaaga actgttgtcc aatgctttat gatttacttc
     1621 aaaccaaaca tatttatatt gtgtgcggaa aaacagattt gcgaaaagga attgatggtc
     1681 ttgccagtct tattcaacaa gagtatcaac tcgagcttta cgaagatgcc gtttttttat
     1741 tttgcgggaa ccgtcaagac cgtttcaaac ttctctattg ggacggtgat ggttttcttc
     1801 tctgttataa gcggatagaa aacggcaagt taaaatggcc tcggacgaaa gacgaagttc
     1861 gaaccttaac gaaccaacag gttcgatggt tgttagaagg attaagtatc gatcaacctc
     1921 gagctatttt gccaggaaaa aaaggagttt tttaagctgg ggggttaaca caaaaaatgt
     1981 gttgaacccc tatttcttgt gcgtttcttg tggtattctg taacaaaatc agttcgattc
     2041 aaggtggtga aaaattttgg aaactacaga tacgttgctt cagttgcttc aagaagccca
     2101 taaaaccaat caagcacaac aacaaaccat tcagaatctt acaactgaaa tccaactatt
     2161 gaatgaaaag gtcaattatt taaccaacaa attatttgga cg
//
LOCUS       DBNIBO010000210         2178 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.221, whole genome shotgun sequence.
ACCESSION   DBNIBO010000210 DBNIBO010000000
VERSION     DBNIBO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.221"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(205..1104)
                     /locus_tag="AC1Y77_002632"
     CDS             complement(205..1104)
                     /locus_tag="AC1Y77_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIQ1576155.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="AC1Y77_002633"
     CDS             complement(1114..1470)
                     /locus_tag="AC1Y77_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HIQ1576156.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="AC1Y77_002634"
     CDS             1825..2121
                     /locus_tag="AC1Y77_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HIQ1576157.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          680 a          418 c          369 g          711 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacat
//
LOCUS       DBNIBO010000211         2139 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.222, whole genome shotgun sequence.
ACCESSION   DBNIBO010000211 DBNIBO010000000
VERSION     DBNIBO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.222"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1023
                     /locus_tag="AC1Y77_002635"
     CDS             <1..1023
                     /locus_tag="AC1Y77_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIQ1576158.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..>2139
                     /locus_tag="AC1Y77_002636"
     CDS             1034..>2139
                     /locus_tag="AC1Y77_002636"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIQ1576159.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVN"
BASE COUNT          766 a          381 c          460 g          532 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataa
//
LOCUS       DBNIBO010000212         2111 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.223, whole genome shotgun sequence.
ACCESSION   DBNIBO010000212 DBNIBO010000000
VERSION     DBNIBO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.223"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(314..589)
                     /locus_tag="AC1Y77_002637"
     CDS             complement(314..589)
                     /locus_tag="AC1Y77_002637"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIQ1576160.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            696..1460
                     /locus_tag="AC1Y77_002638"
     CDS             696..1460
                     /locus_tag="AC1Y77_002638"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIQ1576161.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1583..2086
                     /locus_tag="AC1Y77_002639"
     CDS             1583..2086
                     /locus_tag="AC1Y77_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIQ1576162.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT          671 a          396 c          450 g          594 t
ORIGIN      
        1 cttaatactc aaaactaagc gactttttca caaccagtcc ttaatgcttg atgcagatca
       61 gctttttcgc aaccttatcg acgatgttca aaagcttgca tctacggcat taagtccagc
      121 ctgttgaaaa atatggagag ctatttttca caaccagtcc ttaatgcttg atgcagacca
      181 gctttttcac aaccttattg acggtgtttt atcagctgcc tctcggtatt aagtcattac
      241 atttcaaaat atgagaagct atttggaaaa tgtttctcct taatactcga ggcaaagcgc
      301 ttcaaaaaca accttatccc accacatcaa atttctttcg ttcctcaatc atcggatctt
      361 cttggacatc cacatactgc actcgtccac ttgggctcgg agacttcttg acttcttcga
      421 taaatttttg gatgatgtcg tcatttccga ttgcttcgat tgtgactgag ccgtcgtctt
      481 cgttttttac cgttccggtc acgcctaact gatcagcaac catcttggtc atatagcgaa
      541 atcccacgcc ctggacacgt ccttggacat tcattttaac tttgcgcatg ttcgtcacct
      601 ttctttcctg ttttataaat caatgatacc gattttttta ggatgaaaca agctcaagca
      661 cctattccat gatataatca gttgaggtga ggaatgtgaa agagatccaa tcagtaaaaa
      721 atccaagaat caaagaatta aaaaaactac ataaaaagaa atacagagaa gaaaaggcag
      781 aatatctttt ggaagggttc catctgatcg aagaagctgc gtcgtctgat gcagaaataa
      841 aagaactttt tgtgacacag agaggtatga aagaatgggg agactggctg gctgtaaaca
      901 aagagatttc tacttttcta gtttcagatg aggtcatgac cgctatgtca gatctaccta
      961 caccgcaagg gatcttagca gtgatgacgt taacatctga agatacgcca gatttttcag
     1021 gaggctggct tttactagat aatgtacagg atcctggaaa tgtgggaacg atgatccgta
     1081 cggcagatgc gtttggtatg gctggtgttt tattaggtaa aggaacggca gatatttata
     1141 gtacaaaggt gctgcgaagc atgcagggaa gcaattatca tttgcctgtg ttacaaaaag
     1201 atttggcaga agtgatccct gtaatgaaag gcaaagggtt tacggtgtac ggtacagaat
     1261 taaatgaaga agcacatgca ttagacaaag tagaaaaaac agaggatttc gctataatca
     1321 tgggaaacga aggtcaggga gtaagtcagg aaatcttgtc ttcgactgat cagaacgtat
     1381 atatcccaat gaaaggaaat gcggaatcat tgaatgtcgg agtcgcagca gggatcgttc
     1441 tttatcattt ttctttatga ttaaggaact attaagcttt agctatttta tctagagcga
     1501 ctagaataat ccgaacactg gtggtatact tagatcaatt tcagaagaaa atgaaattca
     1561 aaaaggtggc aaatcgataa ttatgacaga aaaatggcgt gaagacgaag aatatatgtc
     1621 ttatgtacaa gatcttttag aaacagaagc agtgaaaaaa ttagcgaatt acacacagca
     1681 tgttcattcc actcgtttgg agcattccat cagtgtttct tactatagtt atctgctcgc
     1741 aaaaaaatgg ggtggcaatg ctaaagcaac agcgcgtgct ggacttttgc atgatctgtt
     1801 ctactatgat tggcgtacga cgaaatttga cgaggggaca catgcgtata tccatccaag
     1861 aattgctgta aagaatgcag aaaaaatcac cgatttgtcg gatcttgagc gagatatcat
     1921 tttgaagcac atgtggggtg caacgatcgc accgcctaag tataaagaag gatatattgt
     1981 gacattcgtg gacaaatact gcgcagtcaa agaagcggct cagcccatgt ctgcaagtat
     2041 gcgtaagaga tggcaacgtt actttgggaa agaatcctcg atttaacagg taaaaccaat
     2101 aatagaccat a
//
LOCUS       DBNIBO010000213         2079 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.224, whole genome shotgun sequence.
ACCESSION   DBNIBO010000213 DBNIBO010000000
VERSION     DBNIBO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.224"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            97..315
                     /locus_tag="AC1Y77_002640"
     CDS             97..315
                     /locus_tag="AC1Y77_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576163.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            302..>2079
                     /locus_tag="AC1Y77_002641"
     CDS             302..>2079
                     /locus_tag="AC1Y77_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HIQ1576164.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPA"
BASE COUNT          726 a          286 c          427 g          640 t
ORIGIN      
        1 gaaggaatag ataagcaata agaatagaat tgagtcaaat gaaatggagg ggtatctcca
       61 cttcatttga gaactttgaa tataggaggg gttattttga ttaaaaatcg tttaatagat
      121 caagtttggt gtctagatca aaatacagat agtctcgaat taatcgagac tatttgcttc
      181 aacactatag ttctcgattt aagagctgaa aatgataatt gtgtaactca tgtcataatt
      241 aatcaaaaaa tggctcagaa gctctctgag agcctcagaa aatgggctaa gggaggtaac
      301 gatgaaaata attgaaaaaa agattgccaa taaaaaaata aattggttgg ttgttaattt
      361 tcttgttctg atagctatga ttatttttac aagtaatatt gctcatgcga gtattttcaa
      421 ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt atagacctta
      481 cctaaaaaag tacaatggag atttccttag tcagttcggg atttggctcg gatgggctgt
      541 ggtaaaaggc atgtttactg tgacagattc gattcaaaat atgattccag atgtgctgga
      601 tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag ccgtaaatac
      661 aattgtcatt ggattaatga tcctttcatt gatgtttgtt ggttacaaaa tgattactgg
      721 aaaaggaaca gtggatttaa aatcggttgg aatgaacgta gtgatgtcta tagcattaat
      781 acttttaatg ccaaccatga tttctagtgg tattcaattt gctaaaacat tttataagga
      841 ctctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa aacaaggagt
      901 aacagattta gcatatatta ataaaacaga tcagtactca agcattgata aaacggagga
      961 tcgaaataat ttaacacgaa aaaattttca acagacggat ctcacacaag tgttaacgga
     1021 taaagtgatt gataaattgg aaaaagaaaa tccaattgcg gacaatcttc gttatgaatt
     1081 agttgaaaat tctaataatg aatttgttgc tacaaagttt tctgacaact tcttatcaac
     1141 attttctgat agcttgaaat caggctatta ccgataccaa gcaaatctat gggggatttc
     1201 aattgggttg acagcgttag caatcgccta tgtgttcagc gcgtttgtga tcattacagc
     1261 aatactagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcaa cagatattga
     1321 gacaggacag cgttcaaaag ttgttctatc agatattcta caatgttact taactgttgg
     1381 gtttcaagga tttgggttat caatgtttgc tatgtttatc aattttctga actcaggaca
     1441 aggtatctca acaaatattt ttataaaaac aattgcttat atctgtgccg tttttgtttt
     1501 aatcaaaggc agtggaacag tcatgagata ctttggtgtt gatattggac tcaaagaagg
     1561 ctatgggcaa cttgcttctg cttttggaat gggcgcaatg ctgtttagaa aaggatcaaa
     1621 tggatttaat cgtgctaaag gaagcggaga aggagaagat cgaaaaccag aaaagaattt
     1681 cggcgaaact ctatcaaaaa aagctggcaa aactggaaga gctttgggat atgcccatga
     1741 acgtggatta tccggtttag cttcagatgg agctacaatg gctagtgaaa gagcaacaaa
     1801 accatttaag agtatgcgtg atatagctaa tgataccaag aataagttta aagaaggttt
     1861 agatgatggt acagtgtccg ctattaataa aaatagcaaa cctatgttag ctaaaaataa
     1921 agaagatgaa actggaaaat atgctgattc cgtgccaaca cgtctttcag atagaaaaga
     1981 tggtgctaaa gttgaaaatg ccgatagaat tatgtctagt tctgaacgta tgcgtgaagc
     2041 aatgaaaaat aatgctgaat cgaataataa tcctgcaag
//
LOCUS       DBNIBO010000214         2056 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.225, whole genome shotgun sequence.
ACCESSION   DBNIBO010000214 DBNIBO010000000
VERSION     DBNIBO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.225"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            493..714
                     /locus_tag="AC1Y77_002642"
     CDS             493..714
                     /locus_tag="AC1Y77_002642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576165.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="AC1Y77_002643"
     CDS             715..972
                     /locus_tag="AC1Y77_002643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576166.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="AC1Y77_002644"
     CDS             complement(1108..1710)
                     /locus_tag="AC1Y77_002644"
                     /inference="COORDINATES: protein motif:HMM:NF013849.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HIQ1576167.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="AC1Y77_002645"
     CDS             complement(1777..1932)
                     /locus_tag="AC1Y77_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HIQ1576168.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DBNIBO010000215         2034 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.226, whole genome shotgun sequence.
ACCESSION   DBNIBO010000215 DBNIBO010000000
VERSION     DBNIBO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.226"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="AC1Y77_002646"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="AC1Y77_002646"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIQ1576169.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="AC1Y77_002647"
     CDS             complement(1431..1823)
                     /locus_tag="AC1Y77_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIQ1576170.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DBNIBO010000216         2001 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.227, whole genome shotgun sequence.
ACCESSION   DBNIBO010000216 DBNIBO010000000
VERSION     DBNIBO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.227"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..376
                     /locus_tag="AC1Y77_002648"
     CDS             <1..376
                     /locus_tag="AC1Y77_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIQ1576171.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /locus_tag="AC1Y77_002649"
     CDS             424..1824
                     /locus_tag="AC1Y77_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIQ1576172.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DBNIBO010000217         2000 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.228, whole genome shotgun sequence.
ACCESSION   DBNIBO010000217 DBNIBO010000000
VERSION     DBNIBO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.228"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..861
                     /gene="mobL"
                     /locus_tag="AC1Y77_002650"
     CDS             <1..861
                     /gene="mobL"
                     /locus_tag="AC1Y77_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HIQ1576173.1"
                     /translation="HIHIHFAVVEKENTRKPYTEIENGVSYIVPKGKRRQATLDAMKT
                     NYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYK
                     TLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQISKALKEEAKSL
                     YGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVNQMQSIYNERSFR
                     TLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAYEEQQ
                     RRIQMEQERQ"
     gene            873..1208
                     /locus_tag="AC1Y77_002651"
     CDS             873..1208
                     /locus_tag="AC1Y77_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576174.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            1355..1549
                     /locus_tag="AC1Y77_002652"
     CDS             1355..1549
                     /locus_tag="AC1Y77_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576175.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            1581..>2000
                     /locus_tag="AC1Y77_002653"
     CDS             1581..>2000
                     /locus_tag="AC1Y77_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576176.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLE"
BASE COUNT          826 a          294 c          407 g          473 t
ORIGIN      
        1 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
       61 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
      121 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
      181 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
      241 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
      301 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
      361 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
      421 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
      481 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
      541 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
      601 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
      661 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
      721 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
      781 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
      841 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
      901 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
      961 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
     1021 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
     1081 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
     1141 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
     1201 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
     1261 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
     1321 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
     1381 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
     1441 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
     1501 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
     1561 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcggttgg
     1621 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
     1681 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
     1741 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
     1801 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggct
     1861 ataacaagta cggcggactg gatatgacga tcgataatat tccaagaatc aaagaagaaa
     1921 tcgaacgctt tgaaaaaggt gaatcgactt tttcagccgc cacgatccga aaatatcaac
     1981 ggaagttgga aacattagaa
//
LOCUS       DBNIBO010000218         1957 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.229, whole genome shotgun sequence.
ACCESSION   DBNIBO010000218 DBNIBO010000000
VERSION     DBNIBO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.229"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..656
                     /locus_tag="AC1Y77_002654"
     CDS             <1..656
                     /locus_tag="AC1Y77_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576177.1"
                     /translation="FLEELSGEEYRLFLNNNILKLISYAKKANSLSLVDNLDNLLPDK
                     LIYDENEDNSQKQIIIFELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPFF
                     TLINFMLYVKEHLGYDDLVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYKE
                     QNIKTLELIKKNIKYYDPLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
     gene            complement(653..1276)
                     /locus_tag="AC1Y77_002655"
     CDS             complement(653..1276)
                     /locus_tag="AC1Y77_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576178.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            1533..1932
                     /locus_tag="AC1Y77_002656"
                     /pseudo
     CDS             1533..1932
                     /locus_tag="AC1Y77_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          704 a          281 c          318 g          654 t
ORIGIN      
        1 aatttttaga agaattatct ggagaagaat atagattatt cctaaataat aatattttaa
       61 aattaatatc ttatgccaag aaagctaata gcctatcttt agtagataac ttagataatc
      121 ttttacccga taaacttatt tatgatgaga atgaagataa ttcacaaaaa caaataatta
      181 tatttgaatt gtatttaaag tggcttagca gcctgtcagt gtcaccagaa agcaatgatg
      241 atgataaata ttttgcgaac aaactgtttc aatttatatt tccagactat tcttcaatgc
      301 cattttttac attaataaat tttatgctgt atgttaaaga acacttagga tatgatgatt
      361 tagtagaaat gataaaggca tatgataaag aaccttatcc attcaaagga atcaatactg
      421 gttcagatat ggctatgatt tcaggcgaaa ctgtagaaga aattctagct aattacaaag
      481 aacaaaatat aaaaactctg gaattaatta aaaagaatat aaaatactat gatcctttac
      541 tagcgaataa agaaaatatt gataagttat taaatgcaat tgctgattct gatgttagat
      601 ctcctagaat tgatgagtta aaagaagaaa ttttaatgta taaagaactt ttttaattca
      661 tttgattact gttatactca atatcattct taattctgta aatagtttgt ctagtaatat
      721 tgtagttttt agcaattttt gaaatagcta tgccctcatt taaatcctta acgatgtttt
      781 cataaactaa acgtcgttga ggattttttg tatctgcact atagagtttt ggacgacctt
      841 tatagacgcc atttgctttt gcaattttaa tgacttgcgc ctggcgtcgt ttagattcgg
      901 tacgctcttg ctctgcaatc atggccaaaa tttggatgat taaatcttta atgaatttat
      961 ccaaatgagg attgccaatt gcttcagcca tgatcggtag actggtgatg atcagctgca
     1021 cgtttttctt ctttaggtaa ttgactgaag caatgatttc gtcgtaattt cttccaagtc
     1081 ggtcgattgc ttcgacaata aaaatatctt ggtctcggac aaactctaaa gcttcctgga
     1141 aaatcggtcg atgagtaatc gttgctccag attgtttctc gatgaaaatt ttttctgcgc
     1201 cgaacttttt catttcttca atttggcgtt gctcattttg atcaatcgac gaaactcgaa
     1261 tataagcaat tttcaaaatt tttacctact tcctcaaact atttaaaata cactcaaaat
     1321 caacactaat ttttttatac actataaagc tgtcattttt ctagataaat ggcataaatt
     1381 ttcagcgcta agtgacaaaa gcaactaatt tttcatgata gatttttaaa tatctcaatc
     1441 tatctccaaa attagttgct tttgtagcta tagttgacta tgaatatgac aaataattag
     1501 aaaaaactgt gccaattaaa tatcaaaatg acaaattgat gaatcaaatt caaattaagg
     1561 ggggcaaccc tggaagtctt aaatttaccc tcaatgaatg gtattgaaga tgaaaattta
     1621 aggcgtttga ttaatagcct tgtcattgaa ttgtacaagt atcaagcaga atcagaacga
     1681 aaaaaaatta aggaacgtca ggcacaagga atcgaaattg ctaagaaaaa aggcaaattc
     1741 aaaggtcggc aactgaaatt taaaaaaaat gatccacgtt taaagcatgc tttcgatttg
     1801 tttttgaatg gtttatccga taaagaagtt gaagaacaaa ctgggatcaa tcgccgaacg
     1861 tttagaaggt atcgagcaag atataacgtg acagtagatc aaagaaaaaa caatgaaaag
     1921 agggatagtt aatgagtacg gttattttag ctgaaaa
//
LOCUS       DBNIBO010000219         1931 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.230, whole genome shotgun sequence.
ACCESSION   DBNIBO010000219 DBNIBO010000000
VERSION     DBNIBO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.230"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(49..1515)
                     /locus_tag="AC1Y77_002657"
     CDS             complement(49..1515)
                     /locus_tag="AC1Y77_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIQ1576179.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1869..>1931
                     /locus_tag="AC1Y77_002658"
     CDS             1869..>1931
                     /locus_tag="AC1Y77_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIQ1576180.1"
                     /translation="MQNANQRRNFIMTTFTDTLKN"
BASE COUNT          665 a          376 c          328 g          562 t
ORIGIN      
        1 aaacaggaac tgctgcaaat aagcaattcc tgtttttatg ttcattgatt aatgaataga
       61 gcccattagt tttttgattg gttctttcac agtaaatagc aagataccac cgacgattgc
      121 tactgcaccg ataataccga agtaagcaga ttctgttccc ggtgtgtaga agcgtgcaat
      181 ttgtgcattg attgcttgtg aagaagcatc agctaagaac caaatagcga ttgtttgtga
      241 ttcaaatgct cgtggtgcta attttgttgt cacagacaaa ccaactggtg aaagacacaa
      301 ttcagcaata accacgatga agaaacttcc caacaaccac aatggactta cttttccact
      361 tgtaccataa agcatacccg gcaacatcaa tagcaagtac gataatcctg caaataaaag
      421 tccaagagag aatttcacta ctgtagatgg ttgacgttca ccaagtttcg tccacatcgt
      481 tacgaatact ggtgtcagaa taaagatgaa aattggattc aatgattgat accagcttgc
      541 ttggatcgtt acaccaaata gcgtagattg tgtacgttca ctagcaaata aagctaagat
      601 agatgaacct tgttcttgta atgaccagaa aatgattgct gcaaggaata atgggatata
      661 agcccatact cttggttttt cttcagcagt tacatctttt gaagtcagca ttttgataaa
      721 gtaatacaac ggtaagataa tccccaatac actgattgta ttgacaaaaa agtcaattgt
      781 cagatgacca gttagttgtg ccccgccgaa tactgcagcg ataacaacaa gtgcgatgat
      841 caagttacgg atgaatcctt tacgttcttc agttgtcaaa ggattcggcg gtgttgtacc
      901 cactccgtct aatgtcttgc gtccttggat gatgtattgt aataatccaa agaacatccc
      961 aatagcagct aaagagaacc ctaaatgata gttatattct tgaccgattg taccaacgat
     1021 tagtggtgct aataatgccc ctaagttgac acccatgtaa aaaatcgaaa atcctgcatc
     1081 tcttcgcaag tcagtctttg aataaagatg tccgaccatg ccagaaacat ttggtttcaa
     1141 aaaggcggtt ccgataataa tcaatgccat cgaaacaaac aatgtactta caccaaatgg
     1201 taaagccaag acgatgtggc caagcataat gaaaactcca ccgaagaata cagtgcgttt
     1261 tgctcctaac actcggtcag atacccatcc gccgacgata ctagacatgt agatcaaaga
     1321 tccgtagatc gacatgatag ctaatgctgt tgctcgtggt aatccaagtc ccccgttggc
     1381 aactgtatcg tacatataat agataagtaa tgcacgcata ccgtaataac taaatcgttc
     1441 ccacatctca gtgaagaaca aagttgacaa tcccttcggc tgcccgaaga agcccttgtc
     1501 ttgctgtttt tccataatct ataccccaat tctttaaatt ttcagaaaat attatacatc
     1561 tttaccgaaa aatttaacta aaaaacttat ttaagaaata taaactaatt ttctataaaa
     1621 gcaagtaatt tttaatggca atatcataaa aaaaagaaga tagagattta ataattatat
     1681 atcagctgtt tttttcaaat aagcaaaaat tcctaaaaca caacaatgaa agcgataact
     1741 aagaaaatcc ttacataacg atttttctta ttaaaattgt tttttgaact tcgcttattt
     1801 tttgtgagca tatctgttta cttacttttt gtaaggtgct atacttcatt tcgtaatcaa
     1861 ttaaaaaaat gcaaaacgca aatcaaagga gaaattttat tatgacaact tttactgaca
     1921 ctttgaaaaa c
//
LOCUS       DBNIBO010000220         1915 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.231, whole genome shotgun sequence.
ACCESSION   DBNIBO010000220 DBNIBO010000000
VERSION     DBNIBO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.231"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..586)
                     /locus_tag="AC1Y77_002659"
     CDS             complement(<1..586)
                     /locus_tag="AC1Y77_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576181.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKIND"
     gene            complement(1224..1481)
                     /locus_tag="AC1Y77_002660"
     CDS             complement(1224..1481)
                     /locus_tag="AC1Y77_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIQ1576182.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1474..1743)
                     /locus_tag="AC1Y77_002661"
     CDS             complement(1474..1743)
                     /locus_tag="AC1Y77_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIQ1576183.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          623 a          327 c          216 g          749 t
ORIGIN      
        1 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
       61 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
      121 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
      181 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
      241 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
      301 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
      361 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
      421 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
      481 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
      541 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
      601 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
      661 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
      721 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
      781 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
      841 taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa
      901 aatttaaaat agattattaa acctattgct tattctattc agtcatatat ataagcgtca
      961 tcctttagta actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa
     1021 attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta
     1081 cccttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta
     1141 tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg
     1201 accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt
     1261 tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca
     1321 gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata
     1381 tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa
     1441 agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa
     1501 gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat
     1561 gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga
     1621 taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc
     1681 attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc
     1741 cattcctttc accccttatt tatttctata aaacaattgt acattatatt gtacaattaa
     1801 gcaaatgatt aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg
     1861 ataaaaagtg acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttg
//
LOCUS       DBNIBO010000221         1850 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.232, whole genome shotgun sequence.
ACCESSION   DBNIBO010000221 DBNIBO010000000
VERSION     DBNIBO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.232"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            126..1286
                     /locus_tag="AC1Y77_002662"
     CDS             126..1286
                     /locus_tag="AC1Y77_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIQ1576184.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1302..1745
                     /locus_tag="AC1Y77_002663"
     CDS             1302..1745
                     /locus_tag="AC1Y77_002663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1576185.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          640 a          298 c          372 g          540 t
ORIGIN      
        1 tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg gctttcattt
       61 tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg caagggagga
      121 cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt ttgtttttat
      181 taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg gattctttaa
      241 atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca gctgaaattg
      301 aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa ggattcaatc
      361 ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc agccaattag
      421 aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt tcactttatg
      481 atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga acctctttcc
      541 cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta cctaatcgac
      601 ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg actgttttgg
      661 gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat gacacgtctg
      721 ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg ccgtatatga
      781 tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa tcggtaaaaa
      841 aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt cttgcaggaa
      901 cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat cagtatagat
      961 taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg aaaccggtaa
     1021 atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat ggtgtgccgt
     1081 tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct ggtggaactt
     1141 atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt agaaaagtaa
     1201 aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt caaaaaaatg
     1261 gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc acaacaacaa
     1321 aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct aacttggatt
     1381 ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt aggaaagctt
     1441 taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt tttgctgggg
     1501 tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa caaagaatcg
     1561 gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg acagtctatc
     1621 aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt caaacacagc
     1681 aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa aacgctgatt
     1741 tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg gagcattatt
     1801 tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
//
LOCUS       DBNIBO010000222         1848 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.233, whole genome shotgun sequence.
ACCESSION   DBNIBO010000222 DBNIBO010000000
VERSION     DBNIBO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.233"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            225..842
                     /locus_tag="AC1Y77_002664"
     CDS             225..842
                     /locus_tag="AC1Y77_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576186.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            883..1182
                     /locus_tag="AC1Y77_002665"
     CDS             883..1182
                     /locus_tag="AC1Y77_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HIQ1576187.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1216..1383
                     /locus_tag="AC1Y77_002666"
     CDS             1216..1383
                     /locus_tag="AC1Y77_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIQ1576188.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1428..>1848)
                     /locus_tag="AC1Y77_002667"
     CDS             complement(1428..>1848)
                     /locus_tag="AC1Y77_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576189.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          603 a          302 c          365 g          578 t
ORIGIN      
        1 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
       61 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
      121 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
      181 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
      241 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
      301 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
      361 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
      421 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
      481 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
      541 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
      601 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
      661 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
      721 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
      781 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
      841 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
      901 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
      961 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     1021 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     1081 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     1141 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     1201 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     1261 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     1321 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     1381 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     1441 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     1501 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     1561 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     1621 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     1681 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     1741 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     1801 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgat
//
LOCUS       DBNIBO010000223         1685 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.235, whole genome shotgun sequence.
ACCESSION   DBNIBO010000223 DBNIBO010000000
VERSION     DBNIBO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.235"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(141..1433)
                     /locus_tag="AC1Y77_002668"
     CDS             complement(141..1433)
                     /locus_tag="AC1Y77_002668"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIQ1576190.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          483 a          351 c          295 g          556 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaaaaa gaggttgtaa cagaagtggt cagcttcaag aaataagaag
     1621 gaatgtacga aaattgcttc tcaaattttt gtgaaatttc gacttatttt tcgaagaagc
     1681 tactt
//
LOCUS       DBNIBO010000224         1633 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.236, whole genome shotgun sequence.
ACCESSION   DBNIBO010000224 DBNIBO010000000
VERSION     DBNIBO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.236"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="AC1Y77_002669"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="AC1Y77_002669"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIQ1576191.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          562 a          283 c          378 g          410 t
ORIGIN      
        1 acaatctctt ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaa
//
LOCUS       DBNIBO010000225         1630 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.237, whole genome shotgun sequence.
ACCESSION   DBNIBO010000225 DBNIBO010000000
VERSION     DBNIBO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.237"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(183..1502)
                     /locus_tag="AC1Y77_002670"
     CDS             complement(183..1502)
                     /locus_tag="AC1Y77_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HIQ1576192.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPHFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          483 a          300 c          325 g          522 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagtg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt
//
LOCUS       DBNIBO010000226         1593 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.238, whole genome shotgun sequence.
ACCESSION   DBNIBO010000226 DBNIBO010000000
VERSION     DBNIBO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.238"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(124..414)
                     /locus_tag="AC1Y77_002671"
     CDS             complement(124..414)
                     /locus_tag="AC1Y77_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576193.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(622..1313)
                     /locus_tag="AC1Y77_002672"
                     /pseudo
     CDS             complement(622..1313)
                     /locus_tag="AC1Y77_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          427 a          295 c          231 g          640 t
ORIGIN      
        1 catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc
       61 atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt
      121 tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata
      181 ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct
      241 attattataa taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt
      301 caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct
      361 ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt
      421 cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc
      481 tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga
      541 aggacatgta ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt
      601 aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt
      661 atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa
      721 gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct
      781 acatttgttt tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt
      841 tgtaattctg aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga
      901 tgattgattt catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt
      961 ttgctttgta gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct
     1021 gccgattgat ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct
     1081 gcctttgaat cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc
     1141 gcttgctctt tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca
     1201 atatctgtct ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca
     1261 attagacata gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata
     1321 gctccttaat ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc
     1381 gtttttaaat tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc
     1441 ttttttatct atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa
     1501 ataaatattt atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca
     1561 tttcatagat tactgtattt attacaaaaa acg
//
LOCUS       DBNIBO010000227         1516 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.240, whole genome shotgun sequence.
ACCESSION   DBNIBO010000227 DBNIBO010000000
VERSION     DBNIBO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.240"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..344
                     /locus_tag="AC1Y77_002673"
     CDS             <1..344
                     /locus_tag="AC1Y77_002673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576194.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="AC1Y77_002674"
     CDS             425..1045
                     /locus_tag="AC1Y77_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576195.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
BASE COUNT          573 a          201 c          258 g          484 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttc
//
LOCUS       DBNIBO010000228         1515 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.241, whole genome shotgun sequence.
ACCESSION   DBNIBO010000228 DBNIBO010000000
VERSION     DBNIBO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.241"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            287..694
                     /locus_tag="AC1Y77_002675"
     CDS             287..694
                     /locus_tag="AC1Y77_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIQ1576196.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="AC1Y77_002676"
     CDS             708..1109
                     /locus_tag="AC1Y77_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576197.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="AC1Y77_002677"
     CDS             1111..1482
                     /locus_tag="AC1Y77_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576198.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DBNIBO010000229         1490 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.242, whole genome shotgun sequence.
ACCESSION   DBNIBO010000229 DBNIBO010000000
VERSION     DBNIBO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.242"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1101
                     /locus_tag="AC1Y77_002678"
                     /note="aph(2'')-Ia"
     CDS             <1..1101
                     /locus_tag="AC1Y77_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HIQ1576199.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1231..>1490)
                     /locus_tag="AC1Y77_002679"
     CDS             complement(1231..>1490)
                     /locus_tag="AC1Y77_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1576200.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          597 a          139 c          252 g          502 t
ORIGIN      
        1 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
       61 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      121 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      181 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      241 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      301 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactaataag
      361 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      421 aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt
      481 tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa
      541 gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat
      601 tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata
      661 ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta tatagaaagt
      721 tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat
      781 gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg aataattgat
      841 tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt
      901 gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat
      961 attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt
     1021 gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa atggtagaaa agaaatttat
     1081 aaaaggactt ataaagattg attatataat atatgaaaag ctattataaa agacattagt
     1141 attaaatagt ttaaaaaaat gaaaaataat aaaggaagtg agtcaattcc agactcctgt
     1201 gtaaaatgct atacaatgtt tttaccattt ctacttatca aaattgatgt attttcttga
     1261 agaataaatc cattcatcat gtaggtccat aagaacggct ccaattaagc gattggctga
     1321 tgtttgattg gggaagatgc gaataatctt ttctcttctg cgtacttctt gattcagtcg
     1381 ttcaattaga ttggtactct ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa
     1441 ggcgtcttcg aatccatcat ccaatgatgc gcaagctttt gaatattttg
//
LOCUS       DBNIBO010000230         1478 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.243, whole genome shotgun sequence.
ACCESSION   DBNIBO010000230 DBNIBO010000000
VERSION     DBNIBO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.243"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            71..805
                     /locus_tag="AC1Y77_002680"
     CDS             71..805
                     /locus_tag="AC1Y77_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HIQ1576201.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          554 a          236 c          200 g          488 t
ORIGIN      
        1 acaaaaaata agtggttata atggattgtt aatataaaca ttcattataa cctcatagga
       61 gtgggttata atgaacaaaa aaaatataaa agatagtcaa aactttatta cttcaaagca
      121 tcatataaat gaaattttga gaaatgtaca tttaaataca aatgataata ttattgagat
      181 tggttcaggg aaaggtcatt tcacgtttga attagctaaa aggtgtaatt atgtaaccgc
      241 cattgaaata gatcctaaat tatgtaggat aactaaaaac aaacttattg aatatgagaa
      301 cttccaggtt atcaataaag atattttaca atttaagttt cctaaaaata agtcatataa
      361 gatatatgga aatataccct acaatataag tacagatata attcgaaaaa ttgtttttga
      421 aagcacagct acagaaagtt atttaatagt ggaatatgga tttgctaaaa ggttgctaaa
      481 tacaaatcgt tcactagcac tatttttaat gacagaagtt gatatatcca tattaagtaa
      541 aatccctaga gaatactttc atccaaaacc tagagttaat agctcgttaa ttgtattaaa
      601 aagacaccct tcaaaaatat cactcaaaga tagaaaacaa tatgaaaatt ttgttatgaa
      661 atgggttaac aaagaataca taaaactatt ttccaaaaac caattttatc aagccttaaa
      721 atatgcaaga attgacgatt taaacaatat tagctttgaa caattcttgt ctcttttcaa
      781 tagctataaa ttatttaata gataagttaa ggggtgcata aactgcatcc cttaacttat
      841 cttattttgc ttaaacaatc ttggcccgta aactgcataa ctcatatgta aatcgctcct
      901 ttttaggtgg cacaaatgtg aggcattttc actatttccg gcaattactt ccatgtaaag
      961 tataacccac tatactttgc aagcaaagtg tgggctcgcc gaggggtctt gcagacggct
     1021 ctttcggctc ttttgagccg agtatcagcg cacccttatg gtgcaattag ctgatacctg
     1081 tttgacgatt tgctgcccta ccttttcggt aggtatgcaa acctcaaacc aaaataaaaa
     1141 cccccttcga cttcgtcagg gggtttttat ttatataaat ctccgtaaat aatcgcgatt
     1201 taataacatc ttcaatttta tttttttgcg tattttagaa atgttttcac taaaaaatat
     1261 attatacaaa aaatacagta atcgattata attctttgcc agttaaatat catatcgtta
     1321 ttaaaatatc ggaaaaaaat atcaacaaca ataaatgcaa aagaagacca taaaaattgt
     1381 ttattactca tacatactct ccatttctat catttttaaa tttaccagtc atctctcaaa
     1441 aatctcctag ctatttttct tttttaacca cataccgt
//
LOCUS       DBNIBO010000231         1473 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.244, whole genome shotgun sequence.
ACCESSION   DBNIBO010000231 DBNIBO010000000
VERSION     DBNIBO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.244"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            1..217
                     /locus_tag="AC1Y77_002681"
                     /pseudo
     CDS             1..217
                     /locus_tag="AC1Y77_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            208..513
                     /locus_tag="AC1Y77_002682"
                     /pseudo
     CDS             208..513
                     /locus_tag="AC1Y77_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="AC1Y77_002683"
     CDS             533..1336
                     /locus_tag="AC1Y77_002683"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIQ1576202.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          475 a          263 c          350 g          385 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aag
//
LOCUS       DBNIBO010000232         1472 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.245, whole genome shotgun sequence.
ACCESSION   DBNIBO010000232 DBNIBO010000000
VERSION     DBNIBO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.245"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..207)
                     /locus_tag="AC1Y77_002684"
     CDS             complement(<1..207)
                     /locus_tag="AC1Y77_002684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576203.1"
                     /translation="MLIVVFIFPLFSQVTFSMTQPVSSEEHDNLFRATEEFKKYQENI
                     VPETPLASEPITDIDGNQIGWYIQY"
     gene            complement(1084..1200)
                     /locus_tag="AC1Y77_002685"
     CDS             complement(1084..1200)
                     /locus_tag="AC1Y77_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HIQ1576204.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          485 a          248 c          215 g          524 t
ORIGIN      
        1 gtactgtata taccatccta tttggtttcc atcaatatca gtaattggct cactagctaa
       61 tggagtctcc ggaacaatat tttcctgata tttcttaaac tcttctgtag cacgaaaaag
      121 gttatcatgt tcctcacttg aaactggttg agtcatagaa aatgttactt ggctaaacag
      181 agggaaaata aacactacaa tcaacaaagt ataaaaaaaa gcccttcttt ttcatttttt
      241 atcttccaat cacaaattga aaaacttatt cctgcaactt tgagtctagt atcacaatcg
      301 actgactaca actattcatt tacattctac tttaagttcc tccttaacca ttaattttat
      361 taaagatact tgtaatagtg attctcacaa aatttcatga aaaatacttt attactgtat
      421 ctaaaattcc aatacacaac ttatcgacta ttatctgaaa accaaaactt attattcctt
      481 gatgttattc tagcaatagt aactgctttt ttattttttt gcatattttt tcggcgttac
      541 tttttaaagt taactttttc gacaaataga aagagatacg aatcactgat ttttttgaag
      601 aaaagaaatg gagtgagctg aacgttagag atattgggag ctttcgtaac tgcttattaa
      661 acgataatgg ttatttgcaa ccgtattgct cgcttctaca tgaaatattt catgaaatat
      721 ttcgcaaaac ctttgattat gtagttacgt ttccattctt gactaaaaat gtttcaaaaa
      781 cgaacaaagg tgttaaaagg taaagtgatc aatactactg gtcaaaataa gaggttgaaa
      841 gggaattgaa aaataactac agataatact tttataaacg tacgtgcttt gtggttctgt
      901 agttaactat ataactggaa ttagggaatg gtatcaattt gaaaagaaag attctaccaa
      961 tttatcatat gttaaaaatg gaaaatcaac agcagttctt tcggaaacct tacacaaaaa
     1021 ccgattattg aaaatagccg aaaaacgttt ttcggctatt tttttaatta agtttccttc
     1081 ttcttacctt ttcccactat tccgtttcac atctttgatg atcttcagcg cacgttttcg
     1141 tgttttgata tttgggcttt tcaagcggcg gtatgcactt gagatatctg ttttttccat
     1201 atggacgacc cactttcttc tttgattgta gtcatgctaa aaaacacaaa tcgaaacaat
     1261 tacgatttgt gttttaaatg gtatcaaaag ttaaaatgaa agtcaacttg ttaaaacagt
     1321 cgatcagact agctgattga ctcctatctc tggaaataaa ttttcattat tacgttttag
     1381 ctacctaaaa aaggccctct tgtccgttaa ctttaatatc tattgttata gctagtttac
     1441 ctattgaacg tactgtgtgt tcgatagaac ac
//
LOCUS       DBNIBO010000233         1456 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.246, whole genome shotgun sequence.
ACCESSION   DBNIBO010000233 DBNIBO010000000
VERSION     DBNIBO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.246"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1041
                     /locus_tag="AC1Y77_002686"
     CDS             <1..1041
                     /locus_tag="AC1Y77_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HIQ1576205.1"
                     /translation="DEINQKYDETLKNVYSSLIVSNIEETSSSEIKNKLSLLKLKEKE
                     YIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDK
                     INNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREI
                     DREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENV
                     LQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMI
                     FSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKA
                     EQYRRSLELSKD"
     gene            1113..>1456
                     /locus_tag="AC1Y77_002687"
     CDS             1113..>1456
                     /locus_tag="AC1Y77_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576206.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          228 g          419 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaaga
//
LOCUS       DBNIBO010000234         1451 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.247, whole genome shotgun sequence.
ACCESSION   DBNIBO010000234 DBNIBO010000000
VERSION     DBNIBO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.247"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(50..865)
                     /gene="thiD"
                     /locus_tag="AC1Y77_002688"
     CDS             complement(50..865)
                     /gene="thiD"
                     /locus_tag="AC1Y77_002688"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIQ1576207.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1159..1434
                     /locus_tag="AC1Y77_002689"
     CDS             1159..1434
                     /locus_tag="AC1Y77_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576208.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          441 a          292 c          226 g          492 t
ORIGIN      
        1 ttgttccttg tgactcggag ctggacggca ggctcacgac ctctttcttt tactctaatt
       61 caccagctgc ccaatgattg atgggtccat atttgtgccc tacatttatt tcttcacgaa
      121 ttgcacggtt tacatagtct tttgccaatc ggatcgcttg ttcaactgtt tttcctttcg
      181 ctaactcagc tgcaatacat gcagatagcg aatcgccagt tccgttgatc cggtcggtag
      241 gataaaatgg tgcatttaac caaaatgatt ttcctgattc gagcaatacg tagtcattga
      301 ctgtttcttg ctgcgcatct ttagcatgat gtcctttgat cataacgttt tttgcaccca
      361 tatcttgcag ttcttttgct gcttgcacaa aatcttcttc agctgctaat gtccgtccag
      421 tcagcttttc tgcttcataa aaattaggtg tcagtacttc ggccaaaggg acaagctttt
      481 ctttcaagct ttgcaaagca gattcttcta gtaaaagatt tccatgtttc gttacgatta
      541 cgggatccac gactaatggt ccaaaatcat atttttggta attttttaca actgtctcaa
      601 tcaactttga atctgctagc atacctgttt tagctgcacg tatttgaaag tccttcgcaa
      661 tcagttcaaa ttctttgtcg ataaaatctg ttggcaatgt cacacttgct ccgattccgt
      721 aagaattacc tgcaacacat gcggtcatga cagaaacacc gtaaacattt cttgtaaaga
      781 atgtatgcat atctgcttgc atccctgcgc ttccatcgct atctgatcct gcgatcgtca
      841 ttgcttgtac atactgatct tccatcaaat cgattcctcc atttttataa aaagattttt
      901 cataatgctg ttactgtttc tatcatatgt ttttcaaagt atccctcagt aaatatacca
      961 ctaccttttc ttggtaatca aatttatttg aaaaaacatt tgtaataaat cagaaaatag
     1021 ttgtcatttg tttgtattcg ctattctaat aataaataac cactatttga tttatatata
     1081 tttcagtatc cttcttgttt tcatataacc aaaggcgcta tgatttccat atgaaaaaaa
     1141 taaagtcagg tgaaaaaaat gaaacttgag gataaaaaag tcattttgaa gaaaatcaaa
     1201 cgactaccag accaaataga tggctatacg atctgttctt tcaaaaaaga caggagtata
     1261 aaagcttata cgcaaaacac agaaaaagga ataatctatt tcctacatga agaaggttac
     1321 gatacccaaa cctttatgtt tactgaaaaa aaagaatttc ataaacagat gaaaaaactg
     1381 attgaaaaag aatttccaag aagtcataaa ctctatgtga atcagcaatt ttaacttcta
     1441 actgttcttt a
//
LOCUS       DBNIBO010000235         1429 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.248, whole genome shotgun sequence.
ACCESSION   DBNIBO010000235 DBNIBO010000000
VERSION     DBNIBO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.248"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            96..1391
                     /locus_tag="AC1Y77_002690"
     CDS             96..1391
                     /locus_tag="AC1Y77_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIQ1576209.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          284 c          267 g          428 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      661 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      721 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      781 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      841 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      901 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      961 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     1021 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1081 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1141 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1201 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1261 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1321 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt
     1381 taaccaaata aacagaacag aaagaggttt tcttctttct gttctgtcc
//
LOCUS       DBNIBO010000236         1426 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.249, whole genome shotgun sequence.
ACCESSION   DBNIBO010000236 DBNIBO010000000
VERSION     DBNIBO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.249"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            101..1393
                     /locus_tag="AC1Y77_002691"
     CDS             101..1393
                     /locus_tag="AC1Y77_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HIQ1576210.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          464 a          292 c          258 g          412 t
ORIGIN      
        1 atgacaccag catctttttt attttccaaa acatatagaa aagacacctt gaaatccttt
       61 agaattaatt tggcgaacca acccaaaagg aggaatcaag atgtcttaca ctcaccttat
      121 caaagaaaca ttagatattc tagacttatc agttactttt aacgaaaatt gtctcacaaa
      181 agaaaaatac aaagggcaaa tctgtcatat ttatcgtgga aacttgattt atacagccca
      241 agaatgcatc cactgtaaac accagattgc ttcagatatt gtgcgttggg gtacaacaac
      301 cgtccggttg ttaatgaacg atgtttctga ataccgtacc tacctcgaat tgaaaaagca
      361 acgcttcaaa tgcaaagctt gtcagcggac ctttgtcgca gatacttctg ttgcgaaaaa
      421 acattgtttc atcagtgaga aagtccgctg gtctgtcgtg actcgtctga aaaaaaatac
      481 ctcaatgaca gagatcgccg ctcagaaaaa tctatcggtc tcttctgttt accgtattat
      541 gaagcgattt tatcgtccgt tgaatccttt tcggacaccg ttacctaagg tactttgttt
      601 tgatgaattc aaatcagttc ggggtgtttc aggtgctatg agtttcatca tgatggatgg
      661 acaaacacag cgcttattgg acattgtcga gaaccgacaa ctgccttttt taaaacgtta
      721 ttttagtcat ttttcacgag aaattcgtga agcggtcgaa tggatcgtga ttgatatgta
      781 cgctccttat gtatcgctcg ttaaaaagct gtttccaaaa gcacagctga ttattgaccg
      841 gtttcacatc gtccaacata ttggtcgtac ctttcgcaat catcgaatca aagaaacgaa
      901 ccaactatta aaaagcaaag aacaaaaaca ctatcaatta ggtaagcaat taaaaagata
      961 ctggaaactt cttcaaaaag atgaacgcaa gcttgattat actcgacgcc tttggcgacc
     1021 tggcttcaaa gctcatttaa cagagaccga tattgtggat cgcttactga aagggagccc
     1081 tgctttgcgt gtaggctatc agttatatca agactttctt tacgctgtaa aggaacgaga
     1141 ctacgtatcg tttgaagaac tacttaccaa taatatcatg cttcccgaag gctatcagac
     1201 gaccttgaga acgttccaga aatttctgcc acaaatcaaa aatgctttac aacaaagtta
     1261 ttcaaatggc ccacttgaat gtctgaacaa ccatatcaaa gtgttaaaac gaaatgcata
     1321 tggctttcgt agcttttata atttcaaatt gcgcatcatg attcgtcatg gcaacgcatt
     1381 gattttcaac taaaaaagat caagaggaac tgatccactt gatctc
//
LOCUS       DBNIBO010000237         1420 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.250, whole genome shotgun sequence.
ACCESSION   DBNIBO010000237 DBNIBO010000000
VERSION     DBNIBO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.250"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="AC1Y77_002692"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="AC1Y77_002692"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIQ1576211.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          495 a          184 c          236 g          505 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatactata
//
LOCUS       DBNIBO010000238         1409 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.251, whole genome shotgun sequence.
ACCESSION   DBNIBO010000238 DBNIBO010000000
VERSION     DBNIBO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.251"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1359
                     /gene="tnpC"
                     /locus_tag="AC1Y77_002693"
     CDS             <1..1359
                     /gene="tnpC"
                     /locus_tag="AC1Y77_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIQ1576212.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DBNIBO010000239         1402 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.252, whole genome shotgun sequence.
ACCESSION   DBNIBO010000239 DBNIBO010000000
VERSION     DBNIBO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.252"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..378)
                     /locus_tag="AC1Y77_002694"
     CDS             complement(<1..378)
                     /locus_tag="AC1Y77_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIQ1576213.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGA"
     gene            complement(389..>1402)
                     /locus_tag="AC1Y77_002695"
     CDS             complement(389..>1402)
                     /locus_tag="AC1Y77_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIQ1576214.1"
                     /translation="ELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVS
                     AHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQ
                     ERE"
BASE COUNT          365 a          299 c          240 g          498 t
ORIGIN      
        1 ggcgcctact tgttgaatga tcgtccaagc gatcagttcc ccttcacgat cgctatctgt
       61 cgcaataata atggtgtcgg ccgattgaag ttgctgtttc accaccttga attgcttttt
      121 cttatcttta ggaacatgat agtgataatg tgtagggaaa ataggcaaat gttctaatga
      181 ccattgtttc caattttcat cgtacatatc gggcgaatct aattcgacta aatgaccaaa
      241 accatacgtg atcacagttt caccaggaaa caatcgatct tggatttcaa aatacccgtc
      301 ttttcgagtg gctttagaga agctatcggc ataagctttc gcttgtgacg gcttttctgc
      361 taaaataacg gttttcatca tgacgatttc actccctttc ttgttgtctg ccttgttgaa
      421 acaacatggc acgtttttgc tttctgattt cttgttgctg gatctcatgc ttcaattgat
      481 cgttccagtc cttttttact tcaggtataa attcatgttc atgattttta tagcctttgt
      541 agtattgttc tgctttataa gagaaaagaa tctgcgcatt aatatcccct ttttcttgat
      601 aaaaacgatc aaaatcgtac gtaccatcca cgcgttcaca aacttttaat tcttttgcga
      661 cttctctaca aatatctttc aaattaatgg cttctattga atcgttaaca gaaagctgct
      721 ggccaaaaaa cgcatttact ttgtgagcgg aaaccagttg gttcgttaat tgcagattgt
      781 tttctgtctt gtgtatggcc ataatagctt ctggtgtgac cttttcttct tttgcgactt
      841 cttcataaat gattttgtat tcttttggca cttgccaatc attagcgatt cctcgttcac
      901 aacgtacttt ctgattcgta aatggatcga ctgcgcccat taattgttct ttttcgtaga
      961 aaatatgtcc tgcacggtca ttatccacac aaatctcgat ggattgagga aaggcttgtt
     1021 tctttctcaa ttcagatact gcctcaccaa aataatgact gatcacatgg tgttttaacc
     1081 cttccataga gactaaccac gtatcgttta actgatcacg attcagtgca gcatagctaa
     1141 gtaaatcaat actactctca aaaaatttta actgcttagg gtcaccgatc tttaggttaa
     1201 agccatgatt cgctgtcgaa tttttatcta tatgcttata tgtgcctctc tcaccaaaac
     1261 gttcataatc aatgaacgtt ccttgataac tcgcccctac tgtctctttt gtctgtggat
     1321 ctttccataa aaaggcgacc gcatgatcta aacgatcgtc tcgaactaag aaattttgcc
     1381 gttcatattt tggctttagt tc
//
LOCUS       DBNIBO010000240         1400 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.253, whole genome shotgun sequence.
ACCESSION   DBNIBO010000240 DBNIBO010000000
VERSION     DBNIBO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.253"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            65..1366
                     /locus_tag="AC1Y77_002696"
     CDS             65..1366
                     /locus_tag="AC1Y77_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIQ1576215.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          460 a          281 c          257 g          402 t
ORIGIN      
        1 aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca aaggagcgtt
       61 ctgtatgcct gatcttcatc atatcaaact actacttgga atcaaagata aaaacatttt
      121 catcacaaat gtggaatcga agagcattaa gaatatcaag agtcttgttg tttctgcgac
      181 gttaagtaaa gaaattcgtc gctgtccttt gtgtaaacag atgaatcatg aaggcatgat
      241 cgttaagaac ggaaagaaaa aatcgctcat tcaacttaat aaatgtgcga accaactgac
      301 ttatcttgct ttagcgaaac aacgctacca ctgtcgtggc tgccacacct attttacggc
      361 gaatacctac attgtcgata gaaactgctt tatcgctaaa caagtgcgct ataaaatttt
      421 agaagagcta actgaaaaac aagcaatgac gacaattgct aagcattgcg gcgtttcttg
      481 gtcgaccgtt tctcggacac tcgcttctct gatccccatg acaaaagtaa aaagaaattg
      541 gttaccgcgt tgtttattaa tggatgaatt tcgttcactg aaaaatcaag tcggtcccta
      601 ctccttttct tgtatggatg gcgatactgg aaaattactg gatattcttc cttcacggaa
      661 gaaaaaggct ctcgtttctt attttatgca atttgaacga cgcgcacgat tgaaggtaaa
      721 ggtcatcgtg acggacatga atgtttctta tgcctctctg ataaaggaat gttttccaaa
      781 ggcaaaactc gttgtggatc gttttcacat tgtcaaacat ctcattcgta aatttgagga
      841 tattcgcgta cgaatcatga aaagctttga tcgaaacgat ccaacacaag ccaaatatta
      901 tcgacaatta aaggcgttgt ctcggcttct gatcaaacga caggacaagc ttgtttatga
      961 caaatggacc aaatggcgca attttggttg ggcgtatctc accgaaagtg aagtggtgga
     1021 tcgtctgctt tcgatctctg acgaattacg aatcgcctat ttttactatc aagaaattct
     1081 tcaagcgttt cacgacaaag aggcggacac tttctttaaa cttgtaagaa cgatgcccaa
     1141 ttccgttcca aaagaactgc attctataaa aaaagctttt ataaactatg aatccggcat
     1201 tcgtttagcc ctcgaattgc cttattcaaa tgccaaaata gaaaatctgc atacccacat
     1261 caaagcattg aaacgagtcg cttatggctt tcgaagcttt cggaaaatga agaccagaat
     1321 tttcttacta aataatctga ttacctatga atcaaaaaac atctaaagta gaaaaggaat
     1381 tctaccttag atgttcttat
//
LOCUS       DBNIBO010000241         1371 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.254, whole genome shotgun sequence.
ACCESSION   DBNIBO010000241 DBNIBO010000000
VERSION     DBNIBO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.254"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            137..400
                     /locus_tag="AC1Y77_002697"
     CDS             137..400
                     /locus_tag="AC1Y77_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIQ1576216.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            400..672
                     /locus_tag="AC1Y77_002698"
     CDS             400..672
                     /locus_tag="AC1Y77_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIQ1576217.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            749..1078
                     /locus_tag="AC1Y77_002699"
     CDS             749..1078
                     /locus_tag="AC1Y77_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1576218.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1068..1354
                     /locus_tag="AC1Y77_002700"
                     /pseudo
     CDS             1068..1354
                     /locus_tag="AC1Y77_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          516 a          167 c          275 g          413 t
ORIGIN      
        1 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
       61 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      121 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      181 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      241 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      301 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      361 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      421 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      481 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      541 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      601 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac
      661 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
      721 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      781 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      841 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      901 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
      961 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
     1021 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
     1081 tcgttccttc aaaacatgtc attaagtatt taaaaagcta aaagagaagc cattgaaaga
     1141 aaaatttttg aatttaattt atgatgaaat tgcgattgat ccatataagg gtgaacaaaa
     1201 aactggggat ttatcaggta tttggtctag tgggttcaaa tatgcgggca caacatatag
     1261 agtagcttac gagattatag aaaacaaggt tattccagta ttgttatgtg gaacgcatga
     1321 aaacttttat gaacagttga aaaaattaat ctagtgattt gttgtcgttc t
//
LOCUS       DBNIBO010000242         1336 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.255, whole genome shotgun sequence.
ACCESSION   DBNIBO010000242 DBNIBO010000000
VERSION     DBNIBO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.255"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(146..1129)
                     /locus_tag="AC1Y77_002701"
     CDS             complement(146..1129)
                     /locus_tag="AC1Y77_002701"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIQ1576219.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DBNIBO010000243         1311 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.256, whole genome shotgun sequence.
ACCESSION   DBNIBO010000243 DBNIBO010000000
VERSION     DBNIBO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.256"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..258
                     /locus_tag="AC1Y77_002702"
     CDS             <1..258
                     /locus_tag="AC1Y77_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576220.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            465..665
                     /locus_tag="AC1Y77_002703"
                     /pseudo
     CDS             465..665
                     /locus_tag="AC1Y77_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1126..>1311)
                     /locus_tag="AC1Y77_002704"
     CDS             complement(1126..>1311)
                     /locus_tag="AC1Y77_002704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576221.1"
                     /translation="ILYEGVSMSYSFSLVLYEHIPSLQEGNLVLLFVFPEKDIPTAKY
                     RSYQRLVQSPPSCLLEK"
BASE COUNT          446 a          215 c          247 g          403 t
ORIGIN      
        1 ttaaaaagaa aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat
       61 atttcatttc taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac
      121 tggggtattg tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt
      181 tataatgctt ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag
      241 aaaaaaacaa gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag
      301 aatgaaacac attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta
      361 agttttttca tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc
      421 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa
      481 tatttgagaa acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga
      541 tcacctccta ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt
      601 gccagttaga tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga
      661 attgatcaga aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg
      721 ctcagggcaa ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc
      781 actttagaat acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat
      841 ttaaacaagt tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca
      901 aggcaataga tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga
      961 ataaccttct gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta
     1021 ttttattggt tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa
     1081 cgattagttt aactgtggga agctccagac atacgattcg agaagttact tctcgagcag
     1141 acaggaaggg ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc
     1201 tttttccgga aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc
     1261 ataaagaact aatgaaaaag aatatgacat tgaaactcct tcatagagaa t
//
LOCUS       DBNIBO010000244         1302 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.257, whole genome shotgun sequence.
ACCESSION   DBNIBO010000244 DBNIBO010000000
VERSION     DBNIBO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.257"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            39..239
                     /locus_tag="AC1Y77_002705"
     CDS             39..239
                     /locus_tag="AC1Y77_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576222.1"
                     /translation="MNKIIPLLLVLFVGEILMRLSAAVHLNLAVFLVILMFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            255..434
                     /locus_tag="AC1Y77_002706"
                     /pseudo
     CDS             255..434
                     /locus_tag="AC1Y77_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            496..680
                     /locus_tag="AC1Y77_002707"
                     /pseudo
     CDS             496..680
                     /locus_tag="AC1Y77_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            714..1145
                     /locus_tag="AC1Y77_002708"
     CDS             714..1145
                     /locus_tag="AC1Y77_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIQ1576223.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          437 a          198 c          267 g          400 t
ORIGIN      
        1 gcaaagcccg aaaacctaag agaaagaagg agatttatat gaacaaaatt attcccttat
       61 tattagtcct atttgttgga gaaatactta tgcgacttag tgcagctgta catttaaacc
      121 tcgcagtatt tttggtaata ctaatgtttt gtttgatctt ttggcgattt gtattagccc
      181 tcgtaagtct actattcaag atgattgcag ccatattttt cataggatta tttcgatgaa
      241 aggagagagt accaatgcca attgcaatag gaaataaacg gttacccgtg acgttggatg
      301 aaaagagaca aaaagaattg cagtaaccaa agcagaagta cggcaaaagt gaatccagga
      361 ttatgtgtat tgcgttagat ttattgattg cgcaagaaaa agcaggattt gacataccag
      421 cactcaaaaa gtgatcgcac tttgcgcacg tgttaatacg tgttataatt attttgtaag
      481 gaggtatagt gcacaatgcc acttacggga aaagaaatgc ttaagttatt gaaaaaaatg
      541 gctgggttga gcgaaggcaa gagggatttc atcatcatct ttataaggat ggtgttagaa
      601 tcactgttcc tgttcatgcc aatcaagatc ttggccgagg acttgagcga aaaattttaa
      661 aggatgcggg attgaaataa tctcgcattc gattaaagaa aggattgact gaaatgttaa
      721 gtgagccttt agcaaaaacc tatcctgcta tttttagccc agaagaaggt ggtgggtact
      781 ttattgaatt tcctgatgtt caaggtgctt acacaggaat taacgaagac gatatatctt
      841 atggaattgc tatggcacaa gaggtgttag gtatggtttt agcagattat attgagcatg
      901 aagatctact tccagaacca acaccaatca ataagatttc tgttgaagat gattctttta
      961 ctactttaat tcgtgtagat gttgccaaat atttgaaaga cacagaactt gtcaaaaaaa
     1021 ccttaacaat tcctcaatgg gcggataagc taggcaaaag ggctggcata aatttttcag
     1081 tccttctaac agaatcaatt gcagataaag cagataacat tcttcgttct ggaagaaata
     1141 attagaactc aaaagggtca gaaacttaaa aatctttggc ttcttttttt actacaaaga
     1201 tggcttcttt aaattatttg cctgattgat ttaaaaaaat tgcgcaaaga ctacgagcta
     1261 attaagttgc tcgtggtctt tttttatgaa aactttttag at
//
LOCUS       DBNIBO010000245         1293 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.258, whole genome shotgun sequence.
ACCESSION   DBNIBO010000245 DBNIBO010000000
VERSION     DBNIBO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.258"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(154..684)
                     /locus_tag="AC1Y77_002709"
                     /pseudo
     CDS             complement(154..684)
                     /locus_tag="AC1Y77_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..>1293
                     /locus_tag="AC1Y77_002710"
     CDS             728..>1293
                     /locus_tag="AC1Y77_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HIQ1576224.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          404 a          233 c          274 g          382 t
ORIGIN      
        1 cacgatttgt gagaattttt tcgagattcc agcgggtgag tatctcatcg tttcgaccag
       61 cggtcaaacc aaaaccgttt gaccgctgat cgaagcgatg aagttgaaac cctgtggaaa
      121 tctcgccaaa aaaatgtaag tggtagctag gaactaagct tgcaatgcca actcctccat
      181 ctgatttggt gttaaatagt tgatactact atggattcgc ttgcgattgt aaaatccttc
      241 aatgtattga aataacgcta aataagcagc ttcaaagttt tcatacgctt ttgatggata
      301 gacttcttct tttttgagtg aggcgtgaaa cgattcgata ccggcattat cgtagggaca
      361 gcccttacga ctgaaggagt gccgaatgtt caattcattc agctttgcct cataggcttc
      421 gctagtgtac tgagaaccca aatctgagtg gatgatcaag ttctcggagg gtgtttggtt
      481 taagagcgca ttgtcaaggg tttcgataac caattctttc gtcatctgct tgccaaactt
      541 ccaaccgatg actttctttg tgtggaggtc ttgaatagtg gagaggtagg tccagccatc
      601 ttttagggta tgaatatagg taatatctgt cacccatttt tgattgatcg acgggttctg
      661 ttgcaaagtt ttaaatctac tatcaaataa gatagaataa tagaaaaaga tagcaggagg
      721 aataacgatg aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt
      781 gggctactat cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg
      841 tggcattaac gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact
      901 ctatcaaatt tggaaaaaga aaaataaaaa atccttttat tcatggaaaa tggatgaaac
      961 gtacatcaaa attaaaggaa aatggcatta tttgtatcga gccatcgatg cagatggttt
     1021 aaccttggat atttggttac gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa
     1081 gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc acagataaag ccccctctat
     1141 tacaagtgcc tttaagaaac taaaagaata cggcttttat caagggacag aacatcgtac
     1201 cattaaatac ctgaataatt tgattgaaca agaccatcgt ccagtaaaga gacgcaataa
     1261 attctatcga agtttacgca ctgcctctac cac
//
LOCUS       DBNIBO010000246         1290 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.259, whole genome shotgun sequence.
ACCESSION   DBNIBO010000246 DBNIBO010000000
VERSION     DBNIBO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.259"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            84..494
                     /locus_tag="AC1Y77_002711"
     CDS             84..494
                     /locus_tag="AC1Y77_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1576225.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            508..864
                     /locus_tag="AC1Y77_002712"
     CDS             508..864
                     /locus_tag="AC1Y77_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIQ1576226.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          466 a          179 c          239 g          406 t
ORIGIN      
        1 ttttttatat tttattagct aataattggt attttaaaag aaattactgt agaattggat
       61 tataattaaa aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct
      121 gtagaaaagc aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt
      181 ttgccgaacc gttaacagaa agtgagatca aaaagatctt ggcagcgact gaaggaggag
      241 ttcaagatat tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg
      301 aattaacttt aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac
      361 ctattttgat tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat
      421 ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca
      481 tcttaaattc atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt
      541 gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt
      601 gacttagaag aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa
      661 gtgcaagcag aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac
      721 ctttcctgtg taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc
      781 agacgtcctt tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg
      841 gaacagctta tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca
      901 tacagttgta acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt
      961 atttttctac tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga
     1021 tatagagtta aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt
     1081 ttaagtagca aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt
     1141 tagaaatgac atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt
     1201 ctatcgaaaa aaacttcaaa aaagttcttg acaaataaca aatgataagt tatgataaat
     1261 gagttgctgt aagatttcga aaaacgaaat
//
LOCUS       DBNIBO010000247         1274 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.260, whole genome shotgun sequence.
ACCESSION   DBNIBO010000247 DBNIBO010000000
VERSION     DBNIBO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.260"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(127..462)
                     /locus_tag="AC1Y77_002713"
                     /pseudo
     CDS             complement(127..462)
                     /locus_tag="AC1Y77_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            644..1249
                     /locus_tag="AC1Y77_002714"
     CDS             644..1249
                     /locus_tag="AC1Y77_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576227.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          463 a          204 c          204 g          403 t
ORIGIN      
        1 gaaaaattaa aaacacaaga atcgttgatt tattagtatt cttgtgtttt caattttata
       61 cattcaaaaa tagtatctgg aacatccgtg cgaaaatgca cggtaggaac caaaagacga
      121 acattctcaa taattctcta tatttaacgg acgtagtatt ccattcgata attctttttc
      181 atagcggaaa ctatatttgc caacaaaatt aatgtgatca ctaattaatg gaaattgctt
      241 gcgtaacact tcttcggaac agtcgaaccc ttccgtttta atttgttcaa agactttttc
      301 taaatatagt gaattccaat atatgatagc attagttact aatcctaatg cgttgagctg
      361 ttcctccata ccgtcgtaat aagtttgata aagtttgcct tttctaccat aaaatatatt
      421 tcgacataga gcatgacgag cttcaccttt atttaactgt tccgagctag gctctcctaa
      481 ataaacacca aaagaatttt tattgcgctt aatttcattc attcaaaacc tccaaagtaa
      541 attataagta gacttaaaat ttatttttaa aaatgctatg ataacagtaa aactacaagt
      601 aaattttaca tgtaaattat atcatttttt aaaaggagtt tttatggcaa aaattggata
      661 tattagagtg tcttcacaag atcaaaattt agatcgacaa ttggcaatga tggaagacct
      721 taacattgat aaattattcc aagaaaaagc cagtggtaaa gatatccagc gacctgagtt
      781 tcaaaaatta ctaaattata tccgagaagg tgattgtgtc attgttgcat cactggatcg
      841 cctaggcaga gattatgaag acataaaaaa tacagttacc tttatgaaac agaaaaaagt
      901 agcattaaaa atactagatg cacagttttt agattttaat actggaaatg aactcctaga
      961 tactgctatg ttcgatatgt ttttatcatt gctaagttat attgcacaaa acgaacgtga
     1021 gaaaatccgt gaacgtcagc ggcaaggagt attattggcc aaaaaagctg gtcgatataa
     1081 ggggcgacca attgaatatt ctgagaactc aacagatccg caaaaacgat tagtctttaa
     1141 aactgtggtg gacatgttaa atcaaggagt tccagttgca gaaattgcca aagaaaatgg
     1201 tctctctcga ccaaccatat ataaaataaa gaaagcgaat gatttgtaga atttatacta
     1261 tattcattcg cttt
//
LOCUS       DBNIBO010000248         1270 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.261, whole genome shotgun sequence.
ACCESSION   DBNIBO010000248 DBNIBO010000000
VERSION     DBNIBO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.261"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..484
                     /locus_tag="AC1Y77_002715"
     CDS             <1..484
                     /locus_tag="AC1Y77_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIQ1576228.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1270)
                     /locus_tag="AC1Y77_002716"
                     /pseudo
     CDS             complement(691..1270)
                     /locus_tag="AC1Y77_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          407 a          237 c          246 g          380 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc
//
LOCUS       DBNIBO010000249         1262 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.262, whole genome shotgun sequence.
ACCESSION   DBNIBO010000249 DBNIBO010000000
VERSION     DBNIBO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.262"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..923)
                     /locus_tag="AC1Y77_002717"
     CDS             complement(<1..923)
                     /locus_tag="AC1Y77_002717"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIQ1576229.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
     gene            complement(934..1140)
                     /locus_tag="AC1Y77_002718"
     CDS             complement(934..1140)
                     /locus_tag="AC1Y77_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIQ1576230.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          352 a          269 c          208 g          433 t
ORIGIN      
        1 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
       61 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      121 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      181 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      241 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      301 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      361 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      421 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      481 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      541 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      601 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      661 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      721 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      781 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      841 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      901 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      961 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
     1021 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
     1081 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
     1141 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
     1201 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
     1261 ct
//
LOCUS       DBNIBO010000250         1246 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.263, whole genome shotgun sequence.
ACCESSION   DBNIBO010000250 DBNIBO010000000
VERSION     DBNIBO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.263"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            200..742
                     /locus_tag="AC1Y77_002719"
     CDS             200..742
                     /locus_tag="AC1Y77_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576231.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="AC1Y77_002720"
     CDS             898..>1246
                     /locus_tag="AC1Y77_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIQ1576232.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DBNIBO010000251         1169 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.264, whole genome shotgun sequence.
ACCESSION   DBNIBO010000251 DBNIBO010000000
VERSION     DBNIBO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.264"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(63..626)
                     /locus_tag="AC1Y77_002721"
     CDS             complement(63..626)
                     /locus_tag="AC1Y77_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576233.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          386 a          200 c          176 g          407 t
ORIGIN      
        1 ataataaaaa aatcactcta attctcccat aaatattata gattcctaaa gattaaaata
       61 gattattccc tattgtcttt taagtagcgg tagagtgttg ccttgctaat accagtcatt
      121 tccgtaattt cttttacgct ataaacctga cttttataca attttactgc tcgatcaaca
      181 tctttctggt tcactcgtgg acggccacct ttacgacctc tcgcacgtgc acttttcaaa
      241 ccttctttgg tacgttcaac aattaaatca cgttcgaact gactaaatgc ttggaagaca
      301 gtcatcatta agcgtccgtg aggcgtgctg gtatcgaagt tttctttcaa gctcaccagc
      361 ttaacatggt gttcttcaaa ataggtaaca aggtcaatca aatcctttgt actacgaccc
      421 aatcgagaaa aactttctac aattaccgta tctcccggac gaattttatc ttttaaacga
      481 tttagttggg gacgaaccgc tttggttccc gtcattttct cagttaggat ttcattacaa
      541 ttttgttcat tgagtaagtc taattgacga tttagttctt gtgaacgtgt actcactcgg
      601 gcataaccat aaatgtatcc actcatgctt ttcttccttt cgtactttaa ttctggcttg
      661 attgtatcat aaaagggagt tatagataca tagttaatgc aacaagttat gatacaaaat
      721 atgacctctt ttatttaata aaagaggtca tttcattttg tctcataatc gattgttttt
      781 gatacgagtg tgtatacgaa ataaacaaaa aaatcttaag taaatttcta gatacctaat
      841 ttaatcctaa ctagtgtatc ctaaagttaa taacgagttt atcacaatga aaattataaa
      901 atgctcccat attagatata acatgcaata tttgaattag aactacaaaa tttattcgtt
      961 ttataaagta aataatgttg agatactaag gaatagaata ttataaggaa ttgagctgaa
     1021 tgtattggat aaaaaaagaa taactttatt agagatggac tataaaaatg ctcgtaattt
     1081 tcttctgaag tctgaaagct atttgaatgc tcagctacca gaatatatta gttttgacga
     1141 aactttaaaa tctgctaaaa aagttttaa
//
LOCUS       DBNIBO010000252         1162 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.265, whole genome shotgun sequence.
ACCESSION   DBNIBO010000252 DBNIBO010000000
VERSION     DBNIBO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.265"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            86..>1162
                     /locus_tag="AC1Y77_002722"
     CDS             86..>1162
                     /locus_tag="AC1Y77_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIQ1576234.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          322 a          310 c          315 g          215 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg ct
//
LOCUS       DBNIBO010000253         1140 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.266, whole genome shotgun sequence.
ACCESSION   DBNIBO010000253 DBNIBO010000000
VERSION     DBNIBO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.266"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            312..482
                     /locus_tag="AC1Y77_002723"
     CDS             312..482
                     /locus_tag="AC1Y77_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576235.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            496..1098
                     /locus_tag="AC1Y77_002724"
     CDS             496..1098
                     /locus_tag="AC1Y77_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576236.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
BASE COUNT          420 a          139 c          258 g          323 t
ORIGIN      
        1 tatgatacgt gtttgttttt ctgtgctgtt tagtgaatga ttagcagaaa tatagagagt
       61 aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact tttagttggc
      121 ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg agagcgaagc
      181 gaacacttga tcttttaagt tgctatcttt ttaaggtcaa tagagtatac ttatttgtcc
      241 taatatggtt ttagcagtat aattgacttg gtgaataggt catttaagtt gggcataata
      301 ggaggagtaa aatgaaaaaa tttatttatc gagttttaga aaatgacgaa gtggtggcta
      361 tttttaatga gcaacaatat gcgcaagatt ttatcgctta cgaaaagaca atttctgata
      421 agcaatttga aattgaaaaa gtagatatag ctgattggtt attgcaaccg agagaatttt
      481 agaggttggt tgaaaatggc taaaattggt tatgcacgtg tcagtagcaa agaacagaac
      541 ttagatagac aactgaaagc gttagagggc gtttctaagg tcttttcaga caaattaagc
      601 ggtcaatcgg tcgaacgccc acaattacaa gctatgctta actatattcg tgaaggggat
      661 atagttgttg ttactgaatt agatcgatta ggacgaaata ataaagaatt aacagaattg
      721 atgaatcaaa ttcaaattaa gggggcaacc ctggaagtct taaatttacc ctcaatgaat
      781 ggtattgaag atgaaaattt aagacggctg attaataatt tagtgattga attgtataag
      841 taccaagcgg aatctgaacg caaacgaatt aaagaacgcc aagcccaagg aattgaaatt
      901 gctaagaaaa aaggaaaatt caaagggcga caacctaaat tcaaagaaaa tgatccacgt
      961 ttacaacacg ctttcgattt atttttgaat ggttgttccg ataaagaagt tgaagaacaa
     1021 actggaatta atcgccgaac gtttagaagg tatcgatcaa gatacaacgt gacagtagat
     1081 caaagaaaaa aacaatgaaa agagggatag ttaatgagta cggttatttt agctgaaaaa
//
LOCUS       DBNIBO010000254         1134 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.267, whole genome shotgun sequence.
ACCESSION   DBNIBO010000254 DBNIBO010000000
VERSION     DBNIBO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.267"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..810
                     /gene="mobL"
                     /locus_tag="AC1Y77_002725"
     CDS             <1..810
                     /gene="mobL"
                     /locus_tag="AC1Y77_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HIQ1576237.1"
                     /translation="YTEVENGVSYIVPKGKRRQATLDAMKTNYVHELERFSYEKDRMI
                     GMEKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYKTLPDKRSEWNYGDEKRT
                     KLSPYTREKLDELTKYVLTKEPEYQEYIQISKALKEEAKSLYGESKRESKDAEKNALF
                     DLKKRTGNAILTELKRQDSQIKPLIESVDQMQSIYNERSFRTLKQLPTNEYKYSYEEF
                     MKRRKQFQQRVVTQRSLRQLNRTINAHEEKYHASKAYEEQQRRIQMEQERQ"
     gene            831..>1134
                     /locus_tag="AC1Y77_002726"
     CDS             831..>1134
                     /locus_tag="AC1Y77_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576238.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT          466 a          169 c          236 g          263 t
ORIGIN      
        1 tacacggagg ttgaaaatgg tgtgagttat atcgtaccga aaggtaagag aagacaagca
       61 acattagacg caatgaagac caattatgta catgagttag aacgtttttc ttatgaaaag
      121 gatcgaatga ttggtatgga gaaacgaaat ttattgacgc ggaaaagtga tttgcgtaac
      181 accttaacaa aagtggtaaa agagccagcg cgttacgatc gacacgcgat gaacttatta
      241 aatgaagtat ataaaaccct acctgacaaa cgttcggaat ggaattacgg ggatgaaaaa
      301 cgaaccaaac tttcaccata cacaagagag aaactcgatg agttaaccaa atatgtttta
      361 acaaaagaac ctgaatatca agaatatata cagatttcaa aagcattgaa agaagaagca
      421 aaaagtcttt atggggaaag caagagagaa tcaaaggatg cagagaaaaa tgctctcttt
      481 gatttaaaaa agcgcacagg aaatgccatc ctaacagagc tgaagagaca ggatagtcaa
      541 ataaaaccat taatagaaag tgttgatcag atgcaatcta tttacaatga acgatccttt
      601 cgaacgctta aacaattgcc aacgaatgaa tacaaatata gttacgaaga attcatgaag
      661 cgcaggaaac aattccaaca aagagttgtc acgcagcgga gtttgcgtca attaaatcga
      721 acgattaatg cccatgaaga aaaataccac gcaagtaaag catacgaaga gcaacaacga
      781 agaattcaaa tggaacaaga aagacagtaa agacagtagg aggaatagca atgaaacaat
      841 atttagatca atggaaagtt atagaaggat cattaagaga agaacgaatc gaacaacttc
      901 ctgattgttt agaaaaagag catttgttcc agataagaga aatgttgaga aacgaacagt
      961 ttgatccaaa tcaattttta gtcgttgagt acccagctac tggtgtgtat tgttgtaatc
     1021 atgtgaatgg tgaaaaatat tttatcattc aagaatatga gggcaagtta gcaccttatt
     1081 atactacttg ggaaatgaat gaagagggaa tcaataactt tccatgtaaa agca
//
LOCUS       DBNIBO010000255         1128 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.268, whole genome shotgun sequence.
ACCESSION   DBNIBO010000255 DBNIBO010000000
VERSION     DBNIBO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.268"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            139..459
                     /locus_tag="AC1Y77_002727"
     CDS             139..459
                     /locus_tag="AC1Y77_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576239.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            911..1111
                     /locus_tag="AC1Y77_002728"
     CDS             911..1111
                     /locus_tag="AC1Y77_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIQ1576240.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          385 a          189 c          210 g          344 t
ORIGIN      
        1 aaaagaggtt gtgacgtggt cacgacctct ttttgacgat tatgatacaa aaaacatcat
       61 ttatgaacaa aatgtgaata acgggcgttc ttgagatatg attttaattg ggaaaaactt
      121 atcgataagg aggtctttat gaaaaaaaga ctgttatggt gtttgctgtt tctatcccta
      181 agtttaacaa gtcagccagt ttttgctgaa gaaacacagc ggtctcctat tacaggcaca
      241 cgtctgtaca aaaatgttta tgtacagaca acagatcttg attctgcgat tcctgaaaca
      301 gaagaacggt ctttgcagcc aagatttggt tacttttggt actgtccgac ctgtgattat
      361 gaaagttgct ggcatttctt acattctaca gctgcagctt ttgcttttac tcatactcag
      421 aaatatgatg gacatcatcc tgttatttat gaggtataga tatgagcaaa aaagaaaaac
      481 taatctcgtt gatcgtattg gtttgccttg ttcttctggt aactgtcata ggctatcaga
      541 aataccatga gaacatcaga cctactaaag aatcttcccc agcctcgacc gttgaaacag
      601 aaccgataag tagtcagaaa gaataatttt tttagtttaa agaagaacat cagagcaaaa
      661 aaacaccaac tgaacaatat gtcgtttatt ttaaaaaaag ttcggttttt cactttatgt
      721 aatcgctgag aaaacttttt ttaataaaaa aagtcgaaaa actctagctt ttttgtaaac
      781 ggtatgatat actataggag tagtttttgt tcggtaatct gtggtagctt gtttcaaata
      841 aacatcttcc agatatacac cattcaagta attgcaaaat atgtgttgat acacaaggag
      901 gatttttatt atggaaacag gtacagtaaa atggtttaac tcagaaaaag gatttggatt
      961 catcactgca gaaaacggaa acgatgtatt cgttcatttc tcagctatcc aaggcgacgg
     1021 attcaaaact ttagaagaag gccaagcagt aactttcgat gttgaagaag gccaacgcgg
     1081 acctcaagca acaaacgtta ctaaagctta attaacctat actaaaaa
//
LOCUS       DBNIBO010000256         1122 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.269, whole genome shotgun sequence.
ACCESSION   DBNIBO010000256 DBNIBO010000000
VERSION     DBNIBO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.269"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..>1122
                     /locus_tag="AC1Y77_002729"
     rRNA            <1..>1122
                     /locus_tag="AC1Y77_002729"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          295 a          257 c          343 g          227 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt ac
//
LOCUS       DBNIBO010000257         1076 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.270, whole genome shotgun sequence.
ACCESSION   DBNIBO010000257 DBNIBO010000000
VERSION     DBNIBO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.270"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..188)
                     /locus_tag="AC1Y77_002730"
     CDS             complement(<1..188)
                     /locus_tag="AC1Y77_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576241.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYP"
     gene            436..699
                     /locus_tag="AC1Y77_002731"
     CDS             436..699
                     /locus_tag="AC1Y77_002731"
                     /inference="COORDINATES: protein motif:HMM:NF013677.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1576242.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTILKKALTIFAQK"
     gene            771..>1076
                     /locus_tag="AC1Y77_002732"
     CDS             771..>1076
                     /locus_tag="AC1Y77_002732"
                     /inference="COORDINATES: protein motif:HMM:NF024673.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIQ1576243.1"
                     /translation="MEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAP
                     KIHKILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTT
                     "
BASE COUNT          367 a          213 c          207 g          289 t
ORIGIN      
        1 aatggataat cttctgtcgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgctacttt gataagcttt tgccgctttg tctgtgacga caaaagatct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatcgttgaa
      181 tgaaccattt ctgcttcctc ccttctcttt cttttgatat aatgtagtcg caaatcgcac
      241 cactcaatga gttggcggaa aacaattcat tgaaaaaaag agtcctggac aatcacgttc
      301 aggactcttt tacttctttt attcacttaa caactaacac tctgtgagtg aaatggactc
      361 ttcaatgtga tgttaggatg atttcatgga cacgatttgg tagaatctaa ctactaagaa
      421 aaggacgtgt ccacgatggc aaaagccaag aaaaaatttg atgaagactt taagaagatg
      481 atcttagatt taaaccagtc tggccaatcg gtcgaagaac tggcagcgca atatggtatc
      541 gctacacaaa cgatttatcg ttggaagaag cttcatacaa agaatgtagc aaccggcatg
      601 actgaggcag aaatcctagc gatgaaaaag gaaatggccc gcatgcagga ggaaaacact
      661 atcctaaaaa aggctttaac catattcgct caaaagtaaa aatgagtgat gtcttcgaat
      721 ttattcaatc aaatgttcat gatcatgacg taaaacaaat gtgtaccgtt ttggaagcac
      781 cgaaatccag ctactatgac tggaagaaaa agaaaccttc caagcgccaa tcagaaaaca
      841 aacgcttaaa ccgcttgatt tcaggaatct actttgaaaa caagggtatc tatggtgcgc
      901 ctaaaattca taagattctt gttggccgtg gcgagacctt gtccttaaag aaagtacaaa
      961 tactgatgag aaaattaggc ttgcgttcag tcaccctcaa gaagtacaag cctggcaaac
     1021 aagcgaaagt taaaagtgaa ggacgtaaaa acctgttaaa ccaagatttt accact
//
LOCUS       DBNIBO010000258         1062 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.271, whole genome shotgun sequence.
ACCESSION   DBNIBO010000258 DBNIBO010000000
VERSION     DBNIBO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.271"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(37..993)
                     /locus_tag="AC1Y77_002733"
     CDS             complement(37..993)
                     /locus_tag="AC1Y77_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576244.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DBNIBO010000259         1019 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.272, whole genome shotgun sequence.
ACCESSION   DBNIBO010000259 DBNIBO010000000
VERSION     DBNIBO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.272"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..>1019)
                     /locus_tag="AC1Y77_002734"
     rRNA            complement(<1..>1019)
                     /locus_tag="AC1Y77_002734"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          214 a          318 c          231 g          256 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgac
//
LOCUS       DBNIBO010000260         1008 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.273, whole genome shotgun sequence.
ACCESSION   DBNIBO010000260 DBNIBO010000000
VERSION     DBNIBO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.273"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            131..697
                     /locus_tag="AC1Y77_002735"
     CDS             131..697
                     /locus_tag="AC1Y77_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIQ1576245.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            842..929
                     /locus_tag="AC1Y77_002736"
     tRNA            842..929
                     /locus_tag="AC1Y77_002736"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:874..876,aa:Ser,seq:cga)
BASE COUNT          368 a          152 c          203 g          285 t
ORIGIN      
        1 gcaaaaaaaa gttaaagaag actgaaaaaa atttaaaata taacgttttg agtaaaatgt
       61 ttgcaaaaaa tgctaaaaat agggtacaat aaaacaacat atactgcagt aaagaagtgg
      121 gggagtttta atgagaagaa aagtctatac caaggatcaa attctaaaag ctgcttatga
      181 tgttgttgca aaggaagggt ttaaaggatt tactgcgaga aatattgcta aaaagatggg
      241 gatttcgaca cagccgattt atctggaatt caaaaacatg gaagatttaa aaaatacgtt
      301 gctggattct atctttgaac aactaaaaga agatgttttc cctattgaac gtactggtga
      361 taaaatcatt gatctcggtt tgaactatat ctactttgca aaaaagcatc atagtcttta
      421 catggcattg tacattgatg aacatgggga cggaaagaaa atgcatgatt tttcatacaa
      481 ttatttccgt gaaatagtta aagaagatga aagatacaat caattatcgg atgaatatgt
      541 cgatgctctg catactggta catgggtagc agtgactggc ttagcgtcat tgatgtcatc
      601 aaacatcatg catccaacag aagaacaaat cattgcactg atcaaacgga caattgactc
      661 gattttagca ttagataatc ctgaaataat cagatagtgt tctatcagta tgaatttgat
      721 cggtaagtcc gttggctgca aactgtcaat ggactttttt gctaaaaaaa gcgatttctg
      781 ttatttatct attagaaata ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta
      841 cggaaggttg gcagagtggt aatgcaccgg actcgaaatc cggcgaaccg gctaataccg
      901 gcgcgcaggt tcaaatcctg taccttcctt atatcaacga ttagaggttg tgaagaagct
      961 gtcctgcatc aagtaataag aacggccaaa caaaaatagc ttttccta
//
LOCUS       DBNIBO010000261          963 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.274, whole genome shotgun sequence.
ACCESSION   DBNIBO010000261 DBNIBO010000000
VERSION     DBNIBO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.274"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            64..501
                     /locus_tag="AC1Y77_002737"
     CDS             64..501
                     /locus_tag="AC1Y77_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576246.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            494..>963
                     /locus_tag="AC1Y77_002738"
     CDS             494..>963
                     /locus_tag="AC1Y77_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576247.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFT"
BASE COUNT          387 a          145 c          197 g          234 t
ORIGIN      
        1 catggaaact tatgaaatcg aatattggtt aaccaaatta gagaactaaa aaggagaaaa
       61 aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
      121 aaaatggagg atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag
      181 gaaaaattgt cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt
      241 gcgacgtact atcgaacact ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa
      301 gccttactat gcttggaagt aggtgtcaaa ccaagcacta tcaaaccttc cgaatatcaa
      361 gccttagaac tgacttatgc aaaattcgca cagattaaga aagctaaagc aatggctagc
      421 gactggctag atacttttga aagtatcgaa aaaaatggtg cattttatac gattgaatcg
      481 gaggagaaag ataatgaata aggtggttta tcagctaaca acagaagatg tttcgcaatt
      541 tttaacaagc aaagaacact tgaatgaagt aatcagacgg attcaaaaag actttagttg
      601 tatggaagaa atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagcga
      661 aacaagtcaa acgaaggtca cagtcagcca taatcattta gacgtgtata aacgtcgtcc
      721 tgaaacggta aaaaaaggaa cagagcttct acgactagaa acagaaaaat acatcatgaa
      781 tgtgtaccta gaaaggagtg agctggtcga aaactttgtc agctacacga tttgtaggaa
      841 acactattac tcagaatcag aatattattc aattttcgag agcatggaat taatggaaga
      901 agaaatggaa gagctaaaca gcaaaaatgc gacggtagac gattattacc ttgtatttac
      961 tag
//
LOCUS       DBNIBO010000262          943 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.276, whole genome shotgun sequence.
ACCESSION   DBNIBO010000262 DBNIBO010000000
VERSION     DBNIBO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.276"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..>943
                     /locus_tag="AC1Y77_002739"
     rRNA            <1..>943
                     /locus_tag="AC1Y77_002739"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DBNIBO010000263          927 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.277, whole genome shotgun sequence.
ACCESSION   DBNIBO010000263 DBNIBO010000000
VERSION     DBNIBO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.277"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            73..>927
                     /locus_tag="AC1Y77_002740"
     CDS             73..>927
                     /locus_tag="AC1Y77_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIQ1576248.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSV"
BASE COUNT          322 a          161 c          173 g          271 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtc
//
LOCUS       DBNIBO010000264          923 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.278, whole genome shotgun sequence.
ACCESSION   DBNIBO010000264 DBNIBO010000000
VERSION     DBNIBO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.278"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            248..781
                     /locus_tag="AC1Y77_002741"
     CDS             248..781
                     /locus_tag="AC1Y77_002741"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIQ1576249.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          300 a          174 c          164 g          285 t
ORIGIN      
        1 gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac
       61 aactttgcat aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca
      121 caaggaatga gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt
      181 ttttgttaaa tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt
      241 tacagaaatg aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc
      301 ttgttctctt gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt
      361 gatacagcct gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga
      421 agacgtacgc aatgcgccga aattcccaga agtctggcaa actatccaac catattttca
      481 aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg
      541 cttagattat tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag
      601 ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa
      661 catccaatta gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct
      721 gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta
      781 aaagaatact tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca
      841 aggagcaatt tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg
      901 agctggcagg agaacaccgt tta
//
LOCUS       DBNIBO010000265          910 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.279, whole genome shotgun sequence.
ACCESSION   DBNIBO010000265 DBNIBO010000000
VERSION     DBNIBO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.279"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..355
                     /locus_tag="AC1Y77_002742"
     CDS             <1..355
                     /locus_tag="AC1Y77_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576250.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..776
                     /locus_tag="AC1Y77_002743"
     CDS             615..776
                     /locus_tag="AC1Y77_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576251.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQISMYPMS"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DBNIBO010000266          890 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.280, whole genome shotgun sequence.
ACCESSION   DBNIBO010000266 DBNIBO010000000
VERSION     DBNIBO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.280"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..888
                     /locus_tag="AC1Y77_002744"
     CDS             <1..888
                     /locus_tag="AC1Y77_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HIQ1576252.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DBNIBO010000267          879 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.281, whole genome shotgun sequence.
ACCESSION   DBNIBO010000267 DBNIBO010000000
VERSION     DBNIBO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.281"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..300)
                     /locus_tag="AC1Y77_002745"
     CDS             complement(<1..300)
                     /locus_tag="AC1Y77_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HIQ1576253.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="AC1Y77_002746"
                     /pseudo
     CDS             393..742
                     /locus_tag="AC1Y77_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          301 a          162 c          117 g          299 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaa
//
LOCUS       DBNIBO010000268          832 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.282, whole genome shotgun sequence.
ACCESSION   DBNIBO010000268 DBNIBO010000000
VERSION     DBNIBO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.282"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..809
                     /locus_tag="AC1Y77_002747"
     CDS             <1..809
                     /locus_tag="AC1Y77_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HIQ1576254.1"
                     /translation="YTPQTMSNITKSFTEEVTAFNGRELHDRYAAIYMDATYIPLKRK
                     TVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERGLKNVLLFITDGL
                     KGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKEVALEA
                     RGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQIKKYSHR
                     KEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQLIEN"
BASE COUNT          255 a          169 c          187 g          221 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaatataa caaaatcatt tacagaagag gtaacggcgt
       61 ttaatgggcg ggagcttcat gaccgttatg ctgctattta tatggacgca acgtatattc
      121 cgttaaagcg gaaaaccgtc gccaaggaag ctattcatat cgcagttggc attcgcccgg
      181 acggatcaaa ggaagtattg agctatgcga ttgcaccgac tgaatccatc acgatttggg
      241 aggaaatttt attggacctt caagagcgcg gtttgaaaaa tgtcctcctg ttcatcacgg
      301 atggcttaaa ggggatggta ggagcgatta gtcggttcta tcccaaagct cgttttcaac
      361 attgttgtgt acacgtttcc cgtaatatca gtcataaagt gcgtgtcgat gatcgtaagg
      421 aagtctgtga tgattttaaa atggtgtatc aagcctcatc taaagaggtg gcgttggaag
      481 cacgtggtac ttttgcggaa aaatggaaaa ccagctatcc aaaagtggtt gaatcgattc
      541 tttcgaacga tcacttgctc actttctatg atttcccctt ggccatacgc aagagtattt
      601 attctacgaa cttgattgaa tcctttaata aacaaatcaa gaaatacagc caccgcaagg
      661 aacagttcca aaatgaagag tcgatggaac gtttcttagt ctcgtctttt gatacttaca
      721 accaaaaatt cctaggtcgc agtcataaag gtttccaaca ggccgaaggc gagctcgaac
      781 aaatgctaag ccaactgatt gagaattaga aacaatctgc agtagggaag aa
//
LOCUS       DBNIBO010000269          811 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.283, whole genome shotgun sequence.
ACCESSION   DBNIBO010000269 DBNIBO010000000
VERSION     DBNIBO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.283"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..672
                     /locus_tag="AC1Y77_002748"
     CDS             <1..672
                     /locus_tag="AC1Y77_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HIQ1576255.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DBNIBO010000270          791 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.284, whole genome shotgun sequence.
ACCESSION   DBNIBO010000270 DBNIBO010000000
VERSION     DBNIBO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.284"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..780
                     /locus_tag="AC1Y77_002749"
     CDS             <1..780
                     /locus_tag="AC1Y77_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1576256.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          102 g          244 t
ORIGIN      
        1 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
       61 aaaactttta caggaatgat ttttaaattc gccaaccgtc gatatggatt aaaatataat
      121 cccgtaaatg gggtttcttt agcaagaaaa cctaagacaa acgaagaaat aaaaaaagaa
      181 aaagatggtt atttatcaaa agaagaagtt caaatgattg ctagtaaaca aaaaagttca
      241 catcaacagt cacgttattc tttaattact aaatttttgt ttttaactgg actacgttat
      301 ggtgaattga tatctttgac tgaatctgat tatgatggta ataaaattac ggtttctggt
      361 acttatgatt acgagttaaa aattaaaaca acaactaaaa atacaggttc ttatagaacc
      421 attgaattat caagtaatgc taaagaaatc atagatcaat taattgaaga aaacaaatta
      481 attaagaata ataaagataa atacattttc atctctaaaa atggtaaccc gatatctatt
      541 caagcatata atcaatcttt aagagcagta tccaaagaat taaacataga taaaaaagtt
      601 tcttctcaca tgttaaggca ttcccacatt tctctactta cagaattagg aatcccatta
      661 aaagccatta tggatcgtgt aggacatgaa gattcaaaaa caacattaaa gatttatact
      721 catacaacta aaaatatgca aaatcaacta gtagaaaaac taggaaaaat agaaatttaa
      781 attgcccctt t
//
LOCUS       DBNIBO010000271          779 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.285, whole genome shotgun sequence.
ACCESSION   DBNIBO010000271 DBNIBO010000000
VERSION     DBNIBO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.285"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(109..>779)
                     /locus_tag="AC1Y77_002750"
     CDS             complement(109..>779)
                     /locus_tag="AC1Y77_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HIQ1576257.1"
                     /translation="SRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFT
                     QEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPY
                     SLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFV
                     HQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTS
                     GEAA"
BASE COUNT          215 a          180 c          151 g          233 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccgactga
//
LOCUS       DBNIBO010000272          746 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.286, whole genome shotgun sequence.
ACCESSION   DBNIBO010000272 DBNIBO010000000
VERSION     DBNIBO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.286"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(82..513)
                     /locus_tag="AC1Y77_002751"
     CDS             complement(82..513)
                     /locus_tag="AC1Y77_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HIQ1576258.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          254 a           98 c           85 g          309 t
ORIGIN      
        1 tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat tatctgcact
       61 actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga taatatcatt
      121 taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg ttttattata
      181 tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta ataactctgt
      241 cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta taattgtaat
      301 tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag cctgatcaat
      361 taatattggg atactagctg ataagactat ttttataata tttccgatac caattactct
      421 attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag attcgataaa
      481 cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa caaaaatatt
      541 ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa catttgtatt
      601 ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa gctatttgtt
      661 tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat agaatctaaa
      721 taataaattt ttatgtaccg cccctc
//
LOCUS       DBNIBO010000273          726 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.287, whole genome shotgun sequence.
ACCESSION   DBNIBO010000273 DBNIBO010000000
VERSION     DBNIBO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.287"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            71..>726
                     /locus_tag="AC1Y77_002752"
     CDS             71..>726
                     /locus_tag="AC1Y77_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HIQ1576259.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     G"
BASE COUNT          235 a          128 c          150 g          213 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc tggtttttag gctactcctt
      361 cgaggagaaa atcccccctt tctcaacttt tggaaagaat tatgttcgcc gatttagaga
      421 aacgacggtg tttgaggata tttttgccta tattttagaa caagcagtta aagcaggttt
      481 tgtaacagag gataatttgt atctggattc tacccacatc aaagctaacg ccaataaaca
      541 taagtttacg aaagaaatga cccatgatga agcgaaagcc tatcaggatg aattagaaga
      601 tgagatcaat cgtcaacgaa tagaggctgg aaaacgccct tttactttgg acttagaaaa
      661 ggaagtgaag ctgaaagaaa gaaaaataag taaagctgat ccggaaagcg gctactacgt
      721 caaagg
//
LOCUS       DBNIBO010000274          713 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.288, whole genome shotgun sequence.
ACCESSION   DBNIBO010000274 DBNIBO010000000
VERSION     DBNIBO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.288"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..448)
                     /locus_tag="AC1Y77_002753"
     CDS             complement(<1..448)
                     /locus_tag="AC1Y77_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576260.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          123 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aac
//
LOCUS       DBNIBO010000275          696 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.289, whole genome shotgun sequence.
ACCESSION   DBNIBO010000275 DBNIBO010000000
VERSION     DBNIBO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.289"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(53..628)
                     /locus_tag="AC1Y77_002754"
     CDS             complement(53..628)
                     /locus_tag="AC1Y77_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIQ1576261.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DBNIBO010000276          694 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.290, whole genome shotgun sequence.
ACCESSION   DBNIBO010000276 DBNIBO010000000
VERSION     DBNIBO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.290"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..>694
                     /locus_tag="AC1Y77_002755"
     CDS             <1..>694
                     /locus_tag="AC1Y77_002755"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIQ1576262.1"
                     /translation="FSLGRVQTPTLYMIYQLQEKIRNFKKEPYFEGKSEVIAQNGAFD
                     AKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVETEKKSAASPRLFSLSSLQSKM
                     NQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAESI
                     PTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFA
                     EKYTYEETTILTQ"
BASE COUNT          245 a          140 c          144 g          165 t
ORIGIN      
        1 ttttccttag gcagagtcca aaccccaacg ctttacatga tttatcaatt gcaggaaaag
       61 attagaaatt tcaagaaaga accttatttt gaaggaaaat ccgaagtaat cgctcaaaat
      121 ggcgcatttg atgcgaagct tgatccgaat gaaacccaag cgacgcaaga agcatttgaa
      181 gattatttaa aagaaaaggg ggttcaattg ggaaaacagc caggcatgat tcgccaagtt
      241 gaaaccgaga aaaaaagcgc ggcaagtcca cgtctttttt ctctatccag ccttcagtct
      301 aaaatgaatc agctcatgaa agctagtgcc aaagacactt tggaagccat gcaaggcttg
      361 tatgaaggga aatatctaag ctatccacga acagacaccc cttatattac ggaaggggag
      421 tatgcgtatt tgttggatca tttagatgaa tacaagcatt ttttaaaggc cgaatcaatc
      481 ccaaccccta ttcatacgcc gaatagtcgg tatgtgaata acaagaaagt ccaggaacat
      541 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
      601 ctgcagcagg ccatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaaa
      661 tatacctatg aagaaacaac gatcttgacg caag
//
LOCUS       DBNIBO010000277          679 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.291, whole genome shotgun sequence.
ACCESSION   DBNIBO010000277 DBNIBO010000000
VERSION     DBNIBO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.291"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            124..410
                     /locus_tag="AC1Y77_002756"
                     /pseudo
     CDS             124..410
                     /locus_tag="AC1Y77_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DBNIBO010000278          625 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.292, whole genome shotgun sequence.
ACCESSION   DBNIBO010000278 DBNIBO010000000
VERSION     DBNIBO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.292"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            1..625
                     /locus_tag="AC1Y77_002757"
                     /pseudo
     CDS             1..625
                     /locus_tag="AC1Y77_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          214 a          110 c          118 g          183 t
ORIGIN      
        1 atgactaaag tgctgactga agaagtaaat gcatttaaag ttagaactct aaatgataag
       61 tatgtcgcta ttttcatgga tgctacttat attcctttaa aacggcaaac cgtctccaaa
      121 gaagcaatct atattgccat tggtatacga gaagacggca ctaaagaagt actaagttat
      181 gcgattgctc cgactgaatc gacctacgtt tggaatgagt tgttacagga catttactcc
      241 agaggggttc atgatgtctt actgtttatt accgatggct taaaaggcat gaaagatacg
      301 attcaccaga tttatcctaa agctaaatac caacattgct gtgttcacgt ttcccgtaat
      361 attgctcata aagtacgtgt caaagatcga aaagaaatct gtgatgattt taaggctgtt
      421 tatcaagcta gctcaaagga agaggcaaat acctttttag ggagtatgat tgagaagtag
      481 caaaaaactt atcctaaagt gacgcagtca ctgataaaaa atcaagattt attgactttt
      541 tatgagtttc cgcctggtat ccgccgaagc atttactcaa ctaatttaat cgaatctttc
      601 aagaaacaaa tcaagaaata cagcc
//
LOCUS       DBNIBO010000279          603 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.293, whole genome shotgun sequence.
ACCESSION   DBNIBO010000279 DBNIBO010000000
VERSION     DBNIBO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.293"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..323
                     /locus_tag="AC1Y77_002758"
     CDS             <1..323
                     /locus_tag="AC1Y77_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1576263.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          215 a          106 c           75 g          207 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
      421 ttttattaaa ctttttaagt ttctattcta tcaaatattt aaacaatctc tgcatccgaa
      481 aagactacct cgtatagttt tactaacaat atttttatta gcgtgttcaa atcctgattt
      541 cttgtatcaa ttattttctg ttcactttca ataagaaaaa agtctttact tccatggata
      601 ata
//
LOCUS       DBNIBO010000280          568 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.294, whole genome shotgun sequence.
ACCESSION   DBNIBO010000280 DBNIBO010000000
VERSION     DBNIBO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.294"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..>568
                     /locus_tag="AC1Y77_002759"
     CDS             <1..>568
                     /locus_tag="AC1Y77_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIQ1576264.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DBNIBO010000281          558 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.295, whole genome shotgun sequence.
ACCESSION   DBNIBO010000281 DBNIBO010000000
VERSION     DBNIBO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.295"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            27..>558
                     /locus_tag="AC1Y77_002760"
     CDS             27..>558
                     /locus_tag="AC1Y77_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HIQ1576265.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKE"
BASE COUNT          206 a          106 c           90 g          156 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc
      541 aaaccgtatc caaagaag
//
LOCUS       DBNIBO010000282          546 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.296, whole genome shotgun sequence.
ACCESSION   DBNIBO010000282 DBNIBO010000000
VERSION     DBNIBO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.296"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..363
                     /locus_tag="AC1Y77_002761"
     CDS             <1..363
                     /locus_tag="AC1Y77_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576266.1"
                     /translation="FLYNETRVPKKHYKERLSVAVEEMIESGVEMNLIATYYRTLEEL
                     KKQNGQWFFQALLCLEAGVKPNTIKPSEYQALELTYGKFVETKKAKTVSSEWLDYLEN
                     VNKYGACYIMKKEDNENE"
     gene            356..>546
                     /locus_tag="AC1Y77_002762"
     CDS             356..>546
                     /locus_tag="AC1Y77_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576267.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTV"
BASE COUNT          213 a           84 c          115 g          134 t
ORIGIN      
        1 ttcctataca atgagacacg tgttccaaaa aaacattata aggaaagatt gtcggtagct
       61 gtggaagaaa tgattgaatc gggtgtcgaa atgaatctca ttgcgaccta ctatcgaacg
      121 cttgaagaac tgaaaaagca aaatggacaa tggtttttcc aagccttact atgcttggaa
      181 gcaggcgtca agccaaacac tatcaaacct tctgaatacc aagctttaga actaacgtat
      241 gggaaatttg tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt ggactactta
      301 gaaaatgtca acaagtatgg cgcatgttac atcatgaaaa aggaggacaa cgaaaatgaa
      361 taaggtgatt tatcaactaa caacggaaga tgtgtcacac tttttaacaa acaaagaaca
      421 tttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta
      481 ctttattcaa agagtcgaag aacaattgta tttggaaagt gaaacgagtc aaacgaaggt
      541 tacagt
//
LOCUS       DBNIBO010000283          546 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.297, whole genome shotgun sequence.
ACCESSION   DBNIBO010000283 DBNIBO010000000
VERSION     DBNIBO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.297"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            143..400
                     /locus_tag="AC1Y77_002763"
     CDS             143..400
                     /locus_tag="AC1Y77_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576268.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          233 a           67 c           77 g          169 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt gttactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 acttta
//
LOCUS       DBNIBO010000284          535 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.298, whole genome shotgun sequence.
ACCESSION   DBNIBO010000284 DBNIBO010000000
VERSION     DBNIBO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.298"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            5..535
                     /locus_tag="AC1Y77_002764"
                     /pseudo
     CDS             5..535
                     /locus_tag="AC1Y77_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
BASE COUNT          237 a           84 c          118 g           96 t
ORIGIN      
        1 ggtcatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccagag
      121 aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agagcgaatc gaaaataaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 agtagaaaaa cttgaaagac aagaagagat aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg aagtacaagc
      481 aatcattgac agcggacgag tgacgcaatg gaaaaaaatc cgaccattta ttttc
//
LOCUS       DBNIBO010000285          531 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.299, whole genome shotgun sequence.
ACCESSION   DBNIBO010000285 DBNIBO010000000
VERSION     DBNIBO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.299"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..>531
                     /locus_tag="AC1Y77_002765"
     CDS             <1..>531
                     /locus_tag="AC1Y77_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576269.1"
                     /translation="EKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIP
                     QYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFEL
                     NAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVT
                     ELAIYQKRKRIMVPLPV"
BASE COUNT          159 a           82 c          104 g          186 t
ORIGIN      
        1 gaaaaggatc ggatcatcga ctatttagcc gataaagatt acgatttaaa attactcagt
       61 gtgcatcata atggtcaatt tgattattta gatgacgaag tcaaagatat ggatcctgct
      121 attgtgattc ctcagtattt tgcacaactt tcagaagcac tagttgttat agaagcagat
      181 gtctttgctc attttgacta tggttttcgg gtatttggtc tatccgttgc tgaatttaaa
      241 caatatgaag cgcagttttt accgattcta gatcaagtaa tcaaaaataa attggcattc
      301 gaattaaatg ctaagagcgc ttacttgtat gacaatttag cgttatatga atatgtcatc
      361 gatttatatc tttcacgtgg tggtaccttg tttagtgttg gatcagatgg gcattacctt
      421 gaacatttcc gtttccattt cgatgatctt tttgctctgt taaaagcaaa aggtgtgacg
      481 gaattagcga tttatcaaaa aagaaagcga attatggttc cattaccagt c
//
LOCUS       DBNIBO010000286          526 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.300, whole genome shotgun sequence.
ACCESSION   DBNIBO010000286 DBNIBO010000000
VERSION     DBNIBO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.300"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..354
                     /locus_tag="AC1Y77_002766"
     CDS             <1..354
                     /locus_tag="AC1Y77_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIQ1576270.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DBNIBO010000287          507 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.301, whole genome shotgun sequence.
ACCESSION   DBNIBO010000287 DBNIBO010000000
VERSION     DBNIBO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.301"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..132)
                     /locus_tag="AC1Y77_002767"
     CDS             complement(<1..132)
                     /locus_tag="AC1Y77_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIQ1576271.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          182 a           93 c           91 g          141 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactat
//
LOCUS       DBNIBO010000288          488 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.302, whole genome shotgun sequence.
ACCESSION   DBNIBO010000288 DBNIBO010000000
VERSION     DBNIBO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.302"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..>488)
                     /locus_tag="AC1Y77_002768"
     CDS             complement(<1..>488)
                     /locus_tag="AC1Y77_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576272.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGK
                     PF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt
       61 ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt
      121 gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa cttataatta
      181 tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg
      241 ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt
      301 ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat
      361 gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc
      421 aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca
      481 aaaacata
//
LOCUS       DBNIBO010000289          485 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.303, whole genome shotgun sequence.
ACCESSION   DBNIBO010000289 DBNIBO010000000
VERSION     DBNIBO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.303"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..482
                     /locus_tag="AC1Y77_002769"
     CDS             <1..482
                     /locus_tag="AC1Y77_002769"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIQ1576273.1"
                     /translation="LAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQTQLTTE
                     ILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLGQRLLDE
                     ANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPILEKEI"
BASE COUNT          176 a           68 c           92 g          149 t
ORIGIN      
        1 cactagcgta tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt
       61 tttatgaatc gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac
      121 aattgacaac tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta
      181 ttaaggatca tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag
      241 agattcaacg gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac
      301 tcgatgaagc gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt
      361 atgaaaaaaa tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga
      421 tcaaacattt tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct
      481 aaaaa
//
LOCUS       DBNIBO010000290          462 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.304, whole genome shotgun sequence.
ACCESSION   DBNIBO010000290 DBNIBO010000000
VERSION     DBNIBO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.304"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..430)
                     /locus_tag="AC1Y77_002770"
     CDS             complement(<1..430)
                     /locus_tag="AC1Y77_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIQ1576274.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DBNIBO010000291          457 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.305, whole genome shotgun sequence.
ACCESSION   DBNIBO010000291 DBNIBO010000000
VERSION     DBNIBO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.305"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(5..90)
                     /locus_tag="AC1Y77_002771"
     tRNA            complement(5..90)
                     /locus_tag="AC1Y77_002771"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="AC1Y77_002772"
     tRNA            complement(107..178)
                     /locus_tag="AC1Y77_002772"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="AC1Y77_002773"
     tRNA            complement(190..262)
                     /locus_tag="AC1Y77_002773"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="AC1Y77_002774"
     tRNA            complement(280..361)
                     /locus_tag="AC1Y77_002774"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="AC1Y77_002775"
     tRNA            complement(369..441)
                     /locus_tag="AC1Y77_002775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
BASE COUNT          118 a          130 c          113 g           96 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgg
//
LOCUS       DBNIBO010000292          451 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.306, whole genome shotgun sequence.
ACCESSION   DBNIBO010000292 DBNIBO010000000
VERSION     DBNIBO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.306"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..>451
                     /locus_tag="AC1Y77_002776"
     CDS             <1..>451
                     /locus_tag="AC1Y77_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HIQ1576275.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTL"
BASE COUNT          160 a           86 c           90 g          115 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc t
//
LOCUS       DBNIBO010000293          445 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.307, whole genome shotgun sequence.
ACCESSION   DBNIBO010000293 DBNIBO010000000
VERSION     DBNIBO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.307"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            57..>445
                     /locus_tag="AC1Y77_002777"
     CDS             57..>445
                     /locus_tag="AC1Y77_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIQ1576276.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKE"
BASE COUNT          172 a           59 c           94 g          120 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaa
//
LOCUS       DBNIBO010000294          427 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.308, whole genome shotgun sequence.
ACCESSION   DBNIBO010000294 DBNIBO010000000
VERSION     DBNIBO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.308"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..>427)
                     /locus_tag="AC1Y77_002778"
     CDS             complement(<1..>427)
                     /locus_tag="AC1Y77_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIQ1576277.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          125 a           76 c           87 g          139 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaa
//
LOCUS       DBNIBO010000295          415 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.denovo.309, whole genome shotgun sequence.
ACCESSION   DBNIBO010000295 DBNIBO010000000
VERSION     DBNIBO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.denovo.309"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..339)
                     /locus_tag="AC1Y77_002779"
     CDS             complement(<1..339)
                     /locus_tag="AC1Y77_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576278.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DBNIBO010000296        21056 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.030, whole genome shotgun sequence.
ACCESSION   DBNIBO010000296 DBNIBO010000000
VERSION     DBNIBO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.030"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002780"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HIQ1576279.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2042..2227
                     /locus_tag="AC1Y77_002781"
     CDS             2042..2227
                     /locus_tag="AC1Y77_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIQ1576280.1"
                     /translation="MNYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINVQ
                     KMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="AC1Y77_002782"
     CDS             complement(2285..2638)
                     /locus_tag="AC1Y77_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576281.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="AC1Y77_002783"
     CDS             3169..3591
                     /locus_tag="AC1Y77_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIQ1576282.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="AC1Y77_002784"
     CDS             3588..3818
                     /locus_tag="AC1Y77_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576283.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="AC1Y77_002785"
     CDS             4316..4516
                     /locus_tag="AC1Y77_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HIQ1576284.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="AC1Y77_002786"
     CDS             4543..5736
                     /locus_tag="AC1Y77_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1576285.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="AC1Y77_002787"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="AC1Y77_002787"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIQ1576286.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /gene="coaA"
                     /locus_tag="AC1Y77_002788"
     CDS             complement(7428..8351)
                     /gene="coaA"
                     /locus_tag="AC1Y77_002788"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIQ1576287.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="AC1Y77_002789"
     CDS             8547..9152
                     /locus_tag="AC1Y77_002789"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1576288.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="AC1Y77_002790"
     CDS             9460..10476
                     /locus_tag="AC1Y77_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1576289.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="AC1Y77_002791"
     CDS             10617..11918
                     /locus_tag="AC1Y77_002791"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIQ1576290.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="AC1Y77_002792"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="AC1Y77_002792"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIQ1576291.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="AC1Y77_002793"
     CDS             12647..13045
                     /locus_tag="AC1Y77_002793"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIQ1576292.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="AC1Y77_002794"
     CDS             13111..14193
                     /locus_tag="AC1Y77_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIQ1576293.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="AC1Y77_002795"
     CDS             14405..15967
                     /locus_tag="AC1Y77_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1576294.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="AC1Y77_002796"
     CDS             15960..17093
                     /locus_tag="AC1Y77_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576295.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="AC1Y77_002797"
     CDS             17093..18049
                     /locus_tag="AC1Y77_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576296.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="AC1Y77_002798"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="AC1Y77_002798"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIQ1576297.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="AC1Y77_002799"
     CDS             19338..20156
                     /locus_tag="AC1Y77_002799"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1576298.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="AC1Y77_002800"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="AC1Y77_002800"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1576299.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6778 a         3760 c         4523 g         5995 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DBNIBO010000297        21056 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.031, whole genome shotgun sequence.
ACCESSION   DBNIBO010000297 DBNIBO010000000
VERSION     DBNIBO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.031"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002801"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HIQ1576300.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2042..2227
                     /locus_tag="AC1Y77_002802"
     CDS             2042..2227
                     /locus_tag="AC1Y77_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIQ1576301.1"
                     /translation="MNYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINVQ
                     KMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="AC1Y77_002803"
     CDS             complement(2285..2638)
                     /locus_tag="AC1Y77_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576302.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="AC1Y77_002804"
     CDS             3169..3591
                     /locus_tag="AC1Y77_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIQ1576303.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="AC1Y77_002805"
     CDS             3588..3818
                     /locus_tag="AC1Y77_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576304.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="AC1Y77_002806"
     CDS             4316..4516
                     /locus_tag="AC1Y77_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HIQ1576305.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="AC1Y77_002807"
     CDS             4543..5736
                     /locus_tag="AC1Y77_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1576306.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="AC1Y77_002808"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="AC1Y77_002808"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIQ1576307.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /gene="coaA"
                     /locus_tag="AC1Y77_002809"
     CDS             complement(7428..8351)
                     /gene="coaA"
                     /locus_tag="AC1Y77_002809"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIQ1576308.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="AC1Y77_002810"
     CDS             8547..9152
                     /locus_tag="AC1Y77_002810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1576309.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="AC1Y77_002811"
     CDS             9460..10476
                     /locus_tag="AC1Y77_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1576310.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="AC1Y77_002812"
     CDS             10617..11918
                     /locus_tag="AC1Y77_002812"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIQ1576311.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="AC1Y77_002813"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="AC1Y77_002813"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIQ1576312.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="AC1Y77_002814"
     CDS             12647..13045
                     /locus_tag="AC1Y77_002814"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIQ1576313.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="AC1Y77_002815"
     CDS             13111..14193
                     /locus_tag="AC1Y77_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIQ1576314.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="AC1Y77_002816"
     CDS             14405..15967
                     /locus_tag="AC1Y77_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1576315.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="AC1Y77_002817"
     CDS             15960..17093
                     /locus_tag="AC1Y77_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576316.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="AC1Y77_002818"
     CDS             17093..18049
                     /locus_tag="AC1Y77_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576317.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="AC1Y77_002819"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="AC1Y77_002819"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIQ1576318.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="AC1Y77_002820"
     CDS             19338..20156
                     /locus_tag="AC1Y77_002820"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1576319.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="AC1Y77_002821"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="AC1Y77_002821"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1576320.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6777 a         3760 c         4524 g         5995 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DBNIBO010000298        20270 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.035, whole genome shotgun sequence.
ACCESSION   DBNIBO010000298 DBNIBO010000000
VERSION     DBNIBO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.035"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1155
                     /locus_tag="AC1Y77_002822"
     CDS             <1..1155
                     /locus_tag="AC1Y77_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIQ1576321.1"
                     /translation="FCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSIN
                     GELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQ
                     REMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKA
                     GTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDY
                     RNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            1256..1441
                     /locus_tag="AC1Y77_002823"
     CDS             1256..1441
                     /locus_tag="AC1Y77_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIQ1576322.1"
                     /translation="MNYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINVQ
                     KMNIITIKEPDAKTQSR"
     gene            complement(1499..1852)
                     /locus_tag="AC1Y77_002824"
     CDS             complement(1499..1852)
                     /locus_tag="AC1Y77_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576323.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2383..2805
                     /locus_tag="AC1Y77_002825"
     CDS             2383..2805
                     /locus_tag="AC1Y77_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIQ1576324.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2802..3032
                     /locus_tag="AC1Y77_002826"
     CDS             2802..3032
                     /locus_tag="AC1Y77_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576325.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3530..3730
                     /locus_tag="AC1Y77_002827"
     CDS             3530..3730
                     /locus_tag="AC1Y77_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HIQ1576326.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3757..4950
                     /locus_tag="AC1Y77_002828"
     CDS             3757..4950
                     /locus_tag="AC1Y77_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIQ1576327.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5018..6583)
                     /gene="guaA"
                     /locus_tag="AC1Y77_002829"
     CDS             complement(5018..6583)
                     /gene="guaA"
                     /locus_tag="AC1Y77_002829"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIQ1576328.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6642..7565)
                     /gene="coaA"
                     /locus_tag="AC1Y77_002830"
     CDS             complement(6642..7565)
                     /gene="coaA"
                     /locus_tag="AC1Y77_002830"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIQ1576329.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7761..8366
                     /locus_tag="AC1Y77_002831"
     CDS             7761..8366
                     /locus_tag="AC1Y77_002831"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1576330.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8674..9690
                     /locus_tag="AC1Y77_002832"
     CDS             8674..9690
                     /locus_tag="AC1Y77_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIQ1576331.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9831..11132
                     /locus_tag="AC1Y77_002833"
     CDS             9831..11132
                     /locus_tag="AC1Y77_002833"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIQ1576332.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11178..11840
                     /gene="deoC"
                     /locus_tag="AC1Y77_002834"
     CDS             11178..11840
                     /gene="deoC"
                     /locus_tag="AC1Y77_002834"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIQ1576333.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11861..12259
                     /locus_tag="AC1Y77_002835"
     CDS             11861..12259
                     /locus_tag="AC1Y77_002835"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIQ1576334.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12325..13407
                     /locus_tag="AC1Y77_002836"
     CDS             12325..13407
                     /locus_tag="AC1Y77_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIQ1576335.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13619..15181
                     /locus_tag="AC1Y77_002837"
     CDS             13619..15181
                     /locus_tag="AC1Y77_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1576336.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15174..16307
                     /locus_tag="AC1Y77_002838"
     CDS             15174..16307
                     /locus_tag="AC1Y77_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576337.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16307..17263
                     /locus_tag="AC1Y77_002839"
     CDS             16307..17263
                     /locus_tag="AC1Y77_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIQ1576338.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17362..18528
                     /gene="deoB"
                     /locus_tag="AC1Y77_002840"
     CDS             17362..18528
                     /gene="deoB"
                     /locus_tag="AC1Y77_002840"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIQ1576339.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18552..19370
                     /locus_tag="AC1Y77_002841"
     CDS             18552..19370
                     /locus_tag="AC1Y77_002841"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1576340.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19387..20094
                     /gene="deoD"
                     /locus_tag="AC1Y77_002842"
     CDS             19387..20094
                     /gene="deoD"
                     /locus_tag="AC1Y77_002842"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIQ1576341.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6494 a         3638 c         4350 g         5788 t
ORIGIN      
        1 ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag tggagtacta
       61 catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat
      121 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
      181 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
      241 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
      301 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
      361 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
      421 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
      481 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
      541 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
      601 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat
      661 gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg
      721 gattgtaaaa tctgttttaa gtacggttta tactatagcc ctgttagtac tccagcagat
      781 tttcggatgc ttactcctat tgtactggag caagccttta gaaaagctgg aacagaattg
      841 ttagagccat atcttagttt taaagtttat gcaccacagg aatatctttc acgggcatat
      901 aacgatgctc ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc
      961 attattattg gagaaattcc tgctcgatgt attcaagatt atcgcaatga tttaactttt
     1021 tttacaaatg ggcttagtgt ttgtttagca gagctaaaag gatatcaggt taccactggc
     1081 gaacctgttt gccagacccg tcgtctaaat agtcggatag ataaagtaag atatatgttc
     1141 aataaaataa cttagtgcgt tttatgttgt tatataaata tggtttctta ttaaataaga
     1201 tgaaatattc tttaatatag atttgaatta aagtggaaag gaggagattg ttattataaa
     1261 ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca agactacgaa tacgagtgga
     1321 tactatactt aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa ctttaattaa
     1381 tgttcaaaaa atgaatataa taacaatcaa agagccagac gccaagacgc agagccgata
     1441 atttgagaaa tgaaactctc atcttatcgg ctctttttgt ttatctgaat tttactgact
     1501 agccttcaat atttcttgct tcattgatac cttttgctag tgcttccatt aaggatagtt
     1561 ctttgtctgt aaagctatcc atgtattttt ctacctgtaa ccttcgagtg cttttaatca
     1621 aattatctgt aggtaagaaa aattcatcaa tagatacatg gagtaatgat acaagatcat
     1681 acagcacttg tgtacttggg tgttgtcctt tgttttctat atttgttaaa taacgtgggt
     1741 caatttcaat cattgttccc acttgttcac gagtgagacc ccgtttcatt cgagcctctt
     1801 taatcgctaa accaacggct ctaaaatcgt atttatcttc ttttttacgc atactaaatc
     1861 acctctatac attttactgt tcctatataa ccataaacag gtatagaaaa acatgttata
     1921 tagtttatga gttcctatag aacactgtgg aatttattaa tgcgatgaat ccaatttcaa
     1981 atagaaaggt cgctgatttt atttacctat acaactttat aaaatttagt catactccta
     2041 gaaagtagat aaaattggcg acttaatgga gggaaaagtc gccatgagaa agataatctg
     2101 tcatctagtt ctcttaatta agttcttatc aaaaaaactt atccacctgt actggataga
     2161 tatgaccatc aatgtggttt tgcagattac atagatagaa aaatcaaagc atatgaacag
     2221 agaaaacaat ctgtttatgt gcttttttgg tcacttaata aatatctttt aggatttctc
     2281 ttcatttatg caacaaatcc cccgttcgtg tcggggttta gtatgaaggg agataaagaa
     2341 gcacatgcca ctccctttca cgacagaaag ggggtgaaaa gcatgaaacc atcttctttt
     2401 caagaaacca tagaaaatca gtttgactat atctgcaaac aagttattga agatgaacga
     2461 aaagattact tcaaacatct agacagacta tcagcaaaag aagtctcgtt ttctgatata
     2521 gataactacc ttgtcaactc tttttctact gttgatacgt acgtcactga tttccaacta
     2581 tttaaattat ttggattaag aatcaatgtt gaaagtgatt tattaagtga agccctgcgt
     2641 catctttcag agaaaaaacg gaacattatt ttacttcatt actttatgga tatgagtgat
     2701 gtggagattg cggagttact aaatttgaat cgttcgactg tttatcgtca tcgaataagt
     2761 ggactagcca tgattaaaga attcatgaag gagtgtaaag aatgaaaaca gcttatccaa
     2821 gggttccctt ccccctcatt gtaaaggcga cagatggcga cgtggaagca attaatcaga
     2881 ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt acgtcgcatg acagacgaat
     2941 atggaaattc tcatatggtt attgatgaaa ccctacgtgg cagaatggaa acgaaactca
     3001 tctctaagat tttatcgttc gaaattaaat agtcccattt tttcgtggaa gcgtgtcgaa
     3061 tcgtatccac gcttcccgat ttgggaggtt tgttattcca tcaaagcata ttgtgtgttc
     3121 agtaggtttt gataggctaa caagccaaac gttctttgac aaccaaataa aggcaatcga
     3181 atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc atgacctaca aaattagtga
     3241 gcgattcatg ttagtaattc gaaagtaatc tttagcagga ttgctgccat gactttctta
     3301 tcgtgataat gatactcccg tatagccata gtccgagcgt tagaagcgtc acaggcaatg
     3361 ggtacggcta catgagaacc atgcagaagt gaaattcctg tgagctttgc tagagctgtt
     3421 cgattgctaa gatattcttc tcttgtcagt gtattattat aaaactattt gatataatta
     3481 taggtgaata ttggagtttt cctagcctat aattgggaaa ggagtaataa tgataacggt
     3541 cacaaaaaag gatttagttg agctaggata tggaacttct ttttcagcag acatcataaa
     3601 aaaagcaaaa gaattgatga ttacaaaagg gcatatctac tatcaatctc gaaagctaga
     3661 tagagtccct cgtgaagctg ttgaagaaat tctaggaatt cggttttcaa atgaaaacag
     3721 gtctgagtag tttttgcaca gtaaggagta aaaactatgg ttaaagaccc aataaaaaaa
     3781 gcaaagaatg gaacctatta ttttcgtgca aatctaggtt atgattctaa agggaaaaaa
     3841 attcaaaaat atcgtagtgg attcactact aaaaaagaag caagagaaga atattcgaaa
     3901 ctgctattga tgaaaccaga agaattaagt gagaataagg ataaaatgac gtttaaacat
     3961 tatatctttg aaattttcct tccatggtat aagacacagg taaagctcag aacatatgaa
     4021 aatcgcttac caactattaa gaagcatttc tcttattttg ataaaatggc tgtttccgat
     4081 attgagccta ttgatgtgca aaattggcaa ctaaaattat caaagaagtg taagtcttct
     4141 tatgtaagag ctgttcaagg actattttca gttgctatgg atcgagcaat tgtccttggg
     4201 ataaccacca caaatccatc taagattatt ggcaatgtga aaaagcaaaa gtctaaaatt
     4261 gaattttgga caaaagaaga atttgaaaaa gtaatctctc taatttacaa agaagattac
     4321 tatcaacact ttttatttat ctctctgtgg tttttattta tgactggaat gcgaattggt
     4381 gaagctactg ccattcaatg ggaagatatt gattttgact caggagtact aaccatcaac
     4441 aaaacactct attacaagaa tcaatcgaat tatagatttg ttgaaccaaa aacaaaggcg
     4501 agcgttcgtc atattgtatt agataaatgc acattaactt atttatccga atggaaggaa
     4561 gtacaacaaa atctaattca gacagacttt gtaatgagct ataacggtat tcctacccaa
     4621 aaacatacac tatcatatgc tattgaacgc tatgctaagc tggctggtat ccatcgaatc
     4681 agaatccatg ggttacggca ttctcatgct tccttgttaa tcagtatggg agaaaatcca
     4741 cttatcatta aagatcgtct aggacacgaa gatattgaga cgactttagg aacgtatgga
     4801 catctttatc ctaacagtaa tttcgaggta gctaacaaat taaatggagc catttccttt
     4861 aaagaagcaa aacaaaatta tgatacttca ccaaagaatc aatttactgt agactacttg
     4921 cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta aaaagataag aagagccaga
     4981 aacccttggt ataagaggat ttctagctct cctacaacta ttcccactcc actgttgctg
     5041 gtggtttaga agtgatgtca tacacgatac ggttgacgtg ggctacttcg tttacgatac
     5101 gtacagagat tttttgcaag acttcccatg ggatacgggc aaagtcagcg gtcattccgt
     5161 cgattgaagt tacagcacgg ataccgactg tgtagtcgta agtacgtccg tcacccataa
     5221 caccgacaga gcggatccct ggaagaacag tgaaatattg ccagatatca cggtcaagtc
     5281 cagcagctgc gatttcttca cgcaagatag catcagattc gcgtacgatt tctagcttgt
     5341 cttcagtgat ttcgccaaga acacgaatac caagaccagg accagggaat ggctgacgcc
     5401 aaacgattga atcaggcatg cctaactgag tccctaattc acggacttcg tctttgaata
     5461 atgtgtttaa tggttcgatc aattcaaatt gcatatcttc tggcagtcca cctacattat
     5521 ggtgagattt gatcgtctgt gctgtttctg taccagattc gataacgtca gtatacaacg
     5581 ttccttgtgc taggaaagag acgccttctt caccagcaag ttttgttgct tcgtcatcaa
     5641 aaacatatac gaattcattc ccaatgattt tacgtttttg ttctgggtca gatacacctt
     5701 tcaacttgtc taagaagcga tctcttgcat cgactttgat gatgttcaag ccgaattttc
     5761 cgcctagact ttccattact tgttccgctt cgcctttgcg tagcagaccg tggtcaacaa
     5821 agatagatgt caattggtca ccgatcgctt tttgtaaaag aaccccaacg acactagaat
     5881 caacgccacc tgaaagacca agcaagactt ttttgtcacc aacttgttca cggatttttt
     5941 ctacttgcat atcgataaag ttatccattg tccagttacc ttcacagtga caaacatcaa
     6001 aggcaaagtg gcgcagcaat tcgattccgt attcggaatg acgtacttct gcatggaatt
     6061 ggataccata aaaattacgt gttgtgtctt ggatagaagc aatcggacaa tctttgcttg
     6121 ttccgactgt ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg ctcatccata
     6181 ctgtttgttg ttttggtgtt tctttaaaca agacagcttg atcgcttgtt acttctaaca
     6241 aggctttccc atattcgcgg tttttagcag gctctacgga accgccaagt ttgtacgtga
     6301 tcaattgcat accatagcaa atccctaata caggaatacc taaattgaag atctcaggat
     6361 cgatcccaaa tgcattctca tcatatacac tgtttggtcc gccagaaaga atgattccct
     6421 ttggattcat cgcttttact tcttctgcag taatgcggtg gctcaataat tcggaaaaga
     6481 ctccaagatc acgaatacgt cttgtgatca attggttata ctgactaccg tagtctaata
     6541 cgataatctt ttcaacgttc gtcatatcgg caacgtttgt caaatttatt acccctatcc
     6601 ctttaaattt tatctcaaaa tagtcaaact attttaaaat gctaatattt tcgcatgaaa
     6661 atcgaatcga tgatatgatt ttcagtttta tggagaatta tatcggctct tcctcgtgta
     6721 ggcaagatat attcctgtaa atttttcaaa ttcacatttt tccatacatc ttttgccatt
     6781 ttaaaggctt cttcacgatc tccgatagca aaacgataat aataattctc tggattttgg
     6841 aaagccgtat ccagtaacgc accaaaacga tccaaatacc atttttcgat cagcacggga
     6901 tcggcatcga catagacaga aaaatcaaaa aagtcgctga cgtagatttg ctgattcgca
     6961 ggtaattgaa gcgtattgat cccctcaacg atcaagatat caggctgctg gatcaattcg
     7021 tacttgtctg gaatgatatc atacacatca tgtgagtaaa caggtacttt tatctcttcc
     7081 tgaccagatt ttacacggtt caagaaatcg atcagcatct ccatatcata actttcagga
     7141 aatcctttac ggtccatgat tccacgctct tttaatatac gatttggata aagaaatccg
     7201 tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat cgtctgcagc
     7261 agtctggctg ttgtactttt tccaacggct acacttccgg cgatcccgat aataaaaggc
     7321 ggtacttcta cgtatttatg aagaaataaa cctttactca agaacaaaga ttcaaattct
     7381 ttcatataaa gataaatcag atgagcaaga ggtacataga tatctcgaac atcctgcatg
     7441 gaaatgcggt cattcaaact tttgatatta tctagttcat cttgtgtcaa aggtgcttgc
     7501 ccattacgat aaaattctcg ccattcttct cttgccacac ggtagtagtt cattggatca
     7561 ttcatattag cctcctgcgt tctcccatca atggaactat tttaacacaa aataaacgca
     7621 aaccccacca cttaagagag attgccgatc tttttgtata atctttataa tagaaagaaa
     7681 cggagtctta ttcttgactt gcgcacggat acataaaagg atagactgtt gtcatgaaag
     7741 aaatgagaag gatggaagac atgacaaatc attactattc tgaacgccca gatacggcgc
     7801 acgattttga acaatgggct tttgagttaa aaggcagaac ctttcaattt gtaacagatt
     7861 ctggtgtatt ttcacgtgaa acagtggatt atggttcacg tgtcctgatt gatgctttcg
     7921 attgggaaga gttacctgat gaaggaaaga tcttagatgt gggatgcggg tatggaccga
     7981 ttgggttagc tttggcattt gcgacacagc ggtttgttga aatggtcgac attaatgaac
     8041 gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat gttgatatcc
     8101 atcaatcgaa tatctatgaa gcagttcatg aagaaaccta tgcagctatc gtaagcaatc
     8161 cgccgatccg tgctggaaaa aaagttgtac atgaaatttt gacaggcgca tatcctcggt
     8221 tgaagaaggg cggtacactg acaattgtga tccagaagaa acaaggagcg ccgagtgcgc
     8281 aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat aaaggatatt
     8341 atatcatcaa aagtgtgaaa gaataactag ctgaaaaata gtggagcgac gaaagagtgg
     8401 aactgggcgt gcatgtacgg gtacgtatca gttctttttt tgttgagaag tttttaaaaa
     8461 caaagaacta ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt tttttacgat
     8521 aaaataaata agatacagaa cttgtataga aggctgccaa gtcggtagat aaaggaggtt
     8581 tttatcaaaa aacatacttt ccttttttct ctattctttt cttgggggtt ttggtacact
     8641 gatagggaat ttagatagaa gggagccaat aggatgaagc tgaccatcag agatatcgca
     8701 gaaatggcag gcgtatcagt aacaactgtc tcacagattt tgaataacaa aggaagcaga
     8761 tttagtgata agacaagaaa aaaagtgttg tcgatcgtga atgaacatca ttataagccc
     8821 gattatttcg cttcaaatgt gatcaatcga cattcaaaga ccatcggaat gatcgtccct
     8881 gatgtcactg atttcttctt ctccaaactg atcgaaggtg tagagagcta tttgaatcct
     8941 ttgggctatg tgatcatgtt atgcaattct cgtcatagcc aagaaaatga gatcaagtat
     9001 ttacaagagc tttcccaccg ctcagtggac ggaatcttgc tggccacacc aaatatttta
     9061 cctgagaaat atgcattaga cagcggtttt tatcaaaaaa tgccaatcat tttaatcgat
     9121 cgcggattga atcgtcggga taacggacgg cttatcgtaa aggaatatga gggcgcctat
     9181 caagctgttt cgctgtttat tgaaaatggc catacgaaga tcggcatgct gaaggaaacc
     9241 actggatact atcaattaga agaacgtttc aacggatatc gtcatgcgct gaaagatcat
     9301 ggcctgcctt ttaatgggaa atttgttgaa catggagagt tgactgtcca aggtggatat
     9361 actgcatcaa aaaaattact caaacataaa gatatcacgg ctattttttg cgggaatgat
     9421 gccatggcga tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat cccagaagat
     9481 atctcagtca taggattcga tggattaaag ctgtcagaat acatgattcc gcaattgacg
     9541 acagtgcagc agccaagttt tgatattgga ttttatgctg cacgtttcct gatcgatacg
     9601 atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt aattattcgt
     9661 gagagcgtaa aagtattgac aaagggctaa ctcggcggta tattgcttat gtaagcattt
     9721 acaaaggcta gttagtgctt tttttatttt tagtaaacca gtttactaaa gtggtttacc
     9781 tttcctaaaa ataatgtata ataccgaatg aagacaagga ggatcaggca atgcgaatgg
     9841 tggatctgat tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa atcgagtaca
     9901 tcgtcacgaa ctatacgaat ggaaagattc cagattatca agtcagtgca ttattgatgg
     9961 caatttttta tcaagacatg acaaatgaag aaatcacgaa cttaacattg gcgattgcta
    10021 attctggtga tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat aagcattcga
    10081 caggtggcgt tggtgacacg accacattga ttttagcccc cctagtagct agtgtcggag
    10141 tgacagtggc taaaatgtct ggacgcggtc taggttacac aggtggtaca ctagacaagc
    10201 tagaagcaat tcccggattt cagatcgagc tttcggatga agaatttgta cgcattgtta
    10261 atgaaagtaa agtagcggta attggtcaat caggaaactt ggcaccggcg gacaaaaaat
    10321 tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct tccgctgatt gccagttcga
    10381 ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg accactggag
    10441 acggtgcttt catgaagaac atcgaagatg caagacgtct ggcaaagacc atgacgagca
    10501 ttgggaaact ggctaaccga gaaacagtgg cggttatctc agatatgtca gaacctcttg
    10561 gcgaagctat cggaaatagt ctagaagttg tcgaggcaat cgaaacatta caaggaaatg
    10621 gtccagaaga tctagtagaa atgtgctacg ccttaggaag ccaaatggtt gttcttgctg
    10681 gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca agaagctctg gaatcaggta
    10741 aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca gcgatcgtgg
    10801 aacatccaga acgaattttg actgcacgct acacaatgga gcttcctgct aaacaaagcg
    10861 gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg ctaggtgctg
    10921 ggcgtaaaac caaagaagaa gatattgatc acgctgttgg attgaaactg cataaaaaaa
    10981 ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac gacgaagaaa
    11041 tcacttctgt cattgaatta ttatataaaa atattgaaat tggtgaatcg gcaatgaagc
    11101 caacgttgat tcatgacatc atcacagaat aaaggaaaaa agcggacttt accgttttaa
    11161 agaaaagggg aaaagaaatg gaactaaacc gaatgatcga ccatactatt ttgaaagcag
    11221 acgcatctaa agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac catttttatt
    11281 ctgtctgcat caatccagca tgggttgcgc tagcgaaaga acaattacaa ggtgagccag
    11341 tagccgtatg tacagtaatc gggtttcctc taggagcaaa cacacccgag acaaaagcat
    11401 ttgaaacaac agatgccatc aataacggtg cagatgaagt cgatatggtc atcaatatcg
    11461 gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga tatcgaagca gttgttgaag
    11521 cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta gatagagaag
    11581 aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc aaaacatcaa
    11641 ctggtttctc aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt gaaactgtag
    11701 gaccagaaat gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa gcaatggcaa
    11761 tgatcgaagc tggtgctaca agaatcggaa caagcgcagg agtagcaatc gtatctggtt
    11821 caactggtga aggctattaa ttttttcaaa ggagaagctc atggcagaac tcaaacaaga
    11881 atggatcaac acagctattg aagcgttaga taaagcctat gttccttatt cccattttcc
    11941 ggtaggcgct tgtctcgtaa cggaatcagg caagatttat caaggcatca acattgagaa
    12001 tgcatcattt ggattaacga attgtgccga gcgcacagct tttttcaaag cagtatctga
    12061 aggagaaaaa acatttacgc atctagtcgt agcggggcac acccccgatc ctatctctcc
    12121 ttgtggtgca tgtcgacaag taatggcaga attctgttcc cctgatatgc cggttacgct
    12181 agtaggagac aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc cttatgcatt
    12241 tactgaaaaa gatctataac tcatgttcat tcagtcagct gactgttgaa aaataaaaca
    12301 attattttag gaggctttac agaaatgaaa aaagcaaaat tatttggttt aggtgctgtc
    12361 gcactagcag cagggttatt tttaggtgca tgcggcaaca acggctctac tgattcatcc
    12421 ggtggcaaaa gctcatcaga cacaacaact gcagcattga tcacagacac aggtggtgta
    12481 gacgaccgtt cattcaacca gtcagcttgg gaaggactag aaaaatgggg gaaagatcaa
    12541 ggactttcac gaggaaatga tggcttccaa tatttccaat cttcaaatga atcagattac
    12601 atcccaaata ttgaccaagc attgaatgct ggattcaaaa cgatttttgg tattggttat
    12661 aaactaaaac cagcgatcca agaacaagca acaaacaata caggtacgaa ctttgttatt
    12721 atcgatgatg tcatcgatgg attagataac gttgtatctg caactttcaa agacaacgaa
    12781 gcttcttatc ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt agtcggcttt
    12841 atcggcggtg taaaaggtga agtaatcgat cgttttgatg ctggattcaa agccggtgtt
    12901 gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata tgctggtgac
    12961 ttctctgctc cagataaagg acgttcaatt gctcaaggta tgtatgcaca aaacgcagat
    13021 atcatcttcc atgcttcagg cggtacaggt aacggtgtat tccaagaagc aaaatcattg
    13081 aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg accgcgacca atcagacgaa
    13141 ggcgaataca cattaaacgg cgaaaagaaa aacttcacat tgacatctac actgaaagca
    13201 gttggaacag tagtagaaga tttggcacaa aaatcagcgg acggtaaatt ccctggcggc
    13261 gaacacactg tttatggatt gaaagaagac ggtgttggtc taacagaagg acaactttct
    13321 gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg tgacgtaaaa
    13381 gttccagaaa ctcctgaaga aaactaaaac catttctata gccttgttag acacatgttt
    13441 gacaaggcta tggtttgatt agaacaagaa ttgttcattt atccaagctg ccttgtgaca
    13501 gtttggataa gggaacaact ccctgaagta atgtttgttt caaaagaaga acgctcacta
    13561 agcgtgaatc ttagtcaatc gttgccctag atagaaatcg ttaggatgtg aaaaaaaggt
    13621 ggttcaagaa aatattgtca tagaaatgca gaatattacc aaagaatttg gaacattcaa
    13681 ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt tgcttggaga
    13741 aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc ggattattag agccgacatc
    13801 agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca aagccaacca
    13861 attagggatt gggatggtcc atcagcactt tatgcttgtt gatgccttta ctgtaacaga
    13921 aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg cttgaccata aaaaagcacg
    13981 aaaagagatc caaaaagttt ctgaacagta tggattgtca gtcaacccag atgcttacgt
    14041 tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac tttaccgagg
    14101 agcagatgta ctgatttttg atgagccgac agctgtattg acccctcaag aaattgatga
    14161 attaatcgtg atcatgaagg aattagtcaa agaaggcaag tcgatcattt tgattacgca
    14221 taagttagat gaaatcaaag cagtagctga ccgctgtaca gttatccgcc gtggaaaagg
    14281 aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa ttagccgata tgatggttgg
    14341 aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag tcgttttgtc
    14401 tattgaaaat ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa acttgaactt
    14461 agaggttcgt gctggcgaag tacttggtat cgctggaatc gatggaaacg ggcagtcgga
    14521 gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc ggacatatca agctaaaagg
    14581 ggaagacatc actaataaaa aaccccgaaa aatcactgaa catggtgtag gacacgtgcc
    14641 agaagaccgt cataaatacg ggttagtcct agatatgaca ttatctgaaa atatcgcact
    14701 acaaacctat catcaaaaac cttacagtaa aaacggcatg ctgaattatt cagtaatgaa
    14761 tgaacatgcc agagaattga tcgaagaata tgatgttcga acaacgaatg aacttgttcc
    14821 cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg aagtcgaccg
    14881 agatcctgat ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag gtgcgatcga
    14941 atttattcat aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt tactgatcag
    15001 tttcgaatta gaagaaattt taaatgtttc ggatcgtatt gctgttatcc atgaaggaga
    15061 aattgtcggt atcgttgatc cgaaagaaac atctgaaaat gaattaggtc tattgatggc
    15121 gggctatacc ttagaagaag cacgaaaaga gttagaaaag ggggctggcg cacatgaatg
    15181 atcgcagcga aaaattccgt aatttattag tgcctgtact ttcagttgtt ttaggattga
    15241 tcttaggcgc gatattgatg gctgcttttg gttttcagcc catccaaggt tattcttcta
    15301 tgctagatgc agcccttggt tcgcaaagag ggataggtga aacgttaagg caagcaacgc
    15361 cgttgatttt tacagcatta ggtttttctg tagctaactc agctgggttt ttcaatattg
    15421 ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca cttgcgtttc
    15481 cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc ttagctggtg
    15541 cgatggcagc ggctgttcca ggtttacttc gagcattttt tggaacaagt gaagtgatcg
    15601 taacaatcat gatgaactat atcttattat acgtaagcac atttgcttta catgaagtga
    15661 tgcctgaaag ctatcgctca agtttggact cttccaatat gatcagtcaa aatgcctcac
    15721 tgcggttacc atggttaaca caagcttttg gcggctctcg cgtaaactca ggattatttt
    15781 tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact ttaggatttg
    15841 aaatccgttc tgttggattg aatccttttg cttctgagta tgcaggaatg agtagtaaac
    15901 gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc agggcttggc ggtgtggttg
    15961 aaggattagg aacgtatcaa aacttctttg ttcaaacgac ttcgctatct attggatttg
    16021 acggaatggc tgtttccttg cttggttctg gatcagcaat cggtattctt ttatcagcga
    16081 tcttgttcag tattttgaag atcggtggac ttggcatgca gacaggagca ggtgttccgt
    16141 ttgagatcgt caatgtatcg attgctttga ttatcttctt tgtcgggatc aacttcctga
    16201 ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca agaagagatc gttgcgacaa
    16261 tagaatcgaa accaacgaat caaaaccatg aagggggcga actataatgt cgactatcga
    16321 attaattgcc ttgatcctta cttctacact ggtttattca acaccgctaa ttctaacgtc
    16381 actaggcgga actttttctg aacgaagcgg gatcgtaaac gtagggctag agggaatcat
    16441 ggtcatgggt gcttttagtt ccatcgtttt caacttgacg atgagtccat cacttggttc
    16501 tatgacacca tggatcgcta ttttagtggg tggcgtcgta ggtatcctat tttctctgat
    16561 tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt atcagcggga cagtaattaa
    16621 tctgatggca cctgctttag gtgttttctt gatcaaagca tggtatggta aaggtcaaac
    16681 tgataacatc acgcagaatc tggggtattt ttctttccca ggattagcag atattccggt
    16741 cattggacca attttcttta aaaatacatt cttacctgct tattgtgcta ttttgattgc
    16801 tgtcctagct tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc gttcagttgg
    16861 tgaaaatcct caagctgccg acacattagg tatcaatgta tatgctatgc gttattcagg
    16921 cgtactgatt tctggcttgt taggcggtat gggcggtgct gtttttgctc agtcgatttc
    16981 cggtaacttt tcagctggta cgatcgttgg acaagggttc atctccatgg ctgctatgat
    17041 ctttggtaag tggaatcctt taggggcaat gggtgcctca ttgttctttg gttttgctca
    17101 aagcttgagt atcattggcg cacaattacc agttatttct tcgattccgc cagtattgct
    17161 gcaaattgct ccatatttgc tgacaatcat tgtgctggtt gttttcttag ggaaagcttc
    17221 tggtccaaaa gcaaacggga agaactatat caaatcaaaa taatctttta gcgaataaaa
    17281 agggtgggtt acgggaagtg tttttcgtct cgcccttttt gttttaaagg caataaaaat
    17341 aaaaagaaag aaggaataat catgtttaaa cgcgtacatc tgatcgtcat ggattcagta
    17401 ggaattggag aagcaccaga tgccgataag ttcggtgatg ttggttctga tacattagga
    17461 cacattgcga aagaagcagg attgaacgtt ccacatttag agcagcttgg tttaggaacg
    17521 atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc gacgaaatta
    17581 gaagaagttt ctgtagggaa agatacgatg acaggccact gggaaatcat gggattgaat
    17641 atcaaaactc catttcgtgt gtttcctaat ggattccctg atgatctgat caaacaaatc
    17701 gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt atagcggaac agctgttatt
    17761 gacgattacg gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac ttctgccgat
    17821 cctgtccttc agatcgcggc acatgaagaa atcattcctt tagaagaatt atatcggatt
    17881 tgccaattcg ttcgtgatat aacaaaagat gatccgtata tgattggccg aatcattgca
    17941 cgtccgtacg tgggtgaacc aggagacttc acacgaacaa gcaatcgcca tgattatgcg
    18001 ttgaatccat tcggacacac agtccttgat tctttgaaag aagcaggaaa ggacgtgatt
    18061 gctgttggaa agatcaacga tattttcaac ggacaaggaa tcactgaatc tgtccgcacg
    18121 aaaagcaata tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga tttttcagga
    18181 atcagcttta ccaatttagt agattttgat gcattatacg gtcatcgtcg ggatgtagtc
    18241 ggctatgcgc atgcgattga agaatttgac ttacgaattc cagaattgct tgaagcgatg
    18301 gaagaagacg acttgctaat gattacagcc gatcacggaa acgatccgac gtttactggt
    18361 accgatcata cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat gaaagaacaa
    18421 ggggcattgc ctcaaggata ttattcagat atttcagcaa cgattgctga gaactttagt
    18481 gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc tttttaaata
    18541 ggagaaagca aatgaccaag ttaagcgaga tgttacaaga aacgacccag tttatccttg
    18601 ataaaggagt aaaagaaatt gattttggtt tgattctagg ttcaggatta ggtgaattag
    18661 cagatgaaat cgaagatgct gtaatgattt cttatgatga gatcccgttc tttcctgttt
    18721 cgacagtagt tgggcatgca ggaaagctag tttatgggtc tctggcaggc aagaaagtgt
    18781 tagccatgca agggagattc cactactatg aaggtcattc catgcaaaca gtgacctatc
    18841 cagtacgtgt gatggccgga ctaggggccc attcagtcat tgtgactaat gcatgtggtg
    18901 gtgtcaacga atcatttgta ccaggagatt tgatgctgat taccgatcat atcaatttta
    18961 ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat gggccctaga tttccagata
    19021 tgagtcaagc ttatacgcct gactatcaag caacagcaaa agcagcagca gaaaaactag
    19081 gtttggatct taaagaaggc gtatatatgg gctattctgg accaacttat gaaacgcctg
    19141 cggagatccg aatgtctcgg acaataggag ccgatgcagt agggatgtcg actgttccag
    19201 aagtcatcgt agcagctcac agcggattaa aagtgttagg aatctcttgt attaccaact
    19261 tagcagcagg tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca actcagagag
    19321 tcaaacaatc ttttaaagca ttggttaaag aaacattgac attattgtaa taggaggaac
    19381 aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa gattttactt
    19441 ccgggagatc ctttgcgagc taaatatatt gcggagactt ttttggaaga tccagtttgt
    19501 tacaatcagg tacgcggtat gttaggcttt accggcaaat ataaaggaga acgtatttct
    19561 gttcaaggaa caggaatggg aatgccatca gccaccatct atgcccatga attgatccag
    19621 tcgtatggtg taaagaaatt gattcgagta ggtacatgcg gtgcattgtc aaaagacgtc
    19681 catgttcgtg atttagtact agcacaaggt gcagcgacca gttcttcgat gatcgaaaaa
    19741 aatttccaag cctttcattt tcctccaatc agtgatttca atctattact gaaagcatat
    19801 gagatagcta aagaaaaggg ctatacggtt catgtaggaa atgttctttc ggaagattct
    19861 ttctacaaag atgatttgac agagacgttc caattagctg aacttggcgt tttaggagtg
    19921 gaaatggaag cagcggctct ttattactta ggcgcgaaat accatgtgca gacattaagt
    19981 ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa caacagctgc agaacgtcaa
    20041 tctacgttca atgaaatgat cgaagtagga ttggaaacag cgatagcaga ataagaagga
    20101 aatgaaaaag ctgccctgca ttaagtaata agaacatcca agcaaaaata gctcctcatg
    20161 tttttgactt ggctggactt attattgcaa atgtaagctt ttgaacaccg tttaccaaga
    20221 ggttgtgaat ctgccgttta actctgagtc acaaggaaca atttcaaaaa
//
LOCUS       DBNIBO010000299        15163 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.060, whole genome shotgun sequence.
ACCESSION   DBNIBO010000299 DBNIBO010000000
VERSION     DBNIBO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.060"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..655
                     /locus_tag="AC1Y77_002843"
     CDS             <1..655
                     /locus_tag="AC1Y77_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HIQ1576342.1"
                     /translation="FKAEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSFYSKH
                     MNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGYLTYE
                     EYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENIFLL
                     RERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(805..1746)
                     /locus_tag="AC1Y77_002844"
     CDS             complement(805..1746)
                     /locus_tag="AC1Y77_002844"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIQ1576343.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1872..2993
                     /locus_tag="AC1Y77_002845"
     CDS             1872..2993
                     /locus_tag="AC1Y77_002845"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIQ1576344.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3251..4753
                     /gene="eat(A)"
                     /locus_tag="AC1Y77_002846"
     CDS             3251..4753
                     /gene="eat(A)"
                     /locus_tag="AC1Y77_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HIQ1576345.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4820..5347
                     /locus_tag="AC1Y77_002847"
     CDS             4820..5347
                     /locus_tag="AC1Y77_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIQ1576346.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5558..6619
                     /locus_tag="AC1Y77_002848"
     CDS             5558..6619
                     /locus_tag="AC1Y77_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIQ1576347.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6622..7518
                     /gene="murQ"
                     /locus_tag="AC1Y77_002849"
     CDS             6622..7518
                     /gene="murQ"
                     /locus_tag="AC1Y77_002849"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIQ1576348.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7532..9514
                     /locus_tag="AC1Y77_002850"
     CDS             7532..9514
                     /locus_tag="AC1Y77_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIQ1576349.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9579..10025)
                     /gene="nrdI"
                     /locus_tag="AC1Y77_002851"
     CDS             complement(9579..10025)
                     /gene="nrdI"
                     /locus_tag="AC1Y77_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIQ1576350.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10236..11660
                     /locus_tag="AC1Y77_002852"
     CDS             10236..11660
                     /locus_tag="AC1Y77_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HIQ1576351.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11841..12614)
                     /locus_tag="AC1Y77_002853"
     CDS             complement(11841..12614)
                     /locus_tag="AC1Y77_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIQ1576352.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12611..13516)
                     /locus_tag="AC1Y77_002854"
     CDS             complement(12611..13516)
                     /locus_tag="AC1Y77_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIQ1576353.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13670..13990)
                     /locus_tag="AC1Y77_002855"
     CDS             complement(13670..13990)
                     /locus_tag="AC1Y77_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIQ1576354.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14464..15150
                     /locus_tag="AC1Y77_002856"
     CDS             14464..15150
                     /locus_tag="AC1Y77_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HIQ1576355.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4831 a         2676 c         3227 g         4429 t
ORIGIN      
        1 cttcaaagca gaacacgagg cgatgctgaa gcaggcacaa gatgaaccgg caatcgatga
       61 gatttatcat tttgattttt tggaagcaaa agaacttggg ctgaaacata atagttcatt
      121 ttattcgaaa catatgaatt tttcttctag cgaaccaaag acggatctat tttttggtgc
      181 aacgaataat gggagaaaag aacgagcaga aagctataag caggaattta taaaaagaga
      241 tgttacgacc aactatttca ttcttccaca tcgagggaat gaacaacctg gatatcttac
      301 ttatgaagag tacctgaaaa aaacagggga atcgcgcggg attctggaac tgttgcggga
      361 gggtcagcaa ggagtgactt tgcggacatt tgaatccatg tattttcaaa agaaactagt
      421 gacggataat caagcgattg ctcattatca tttttatcgt cctgaaaata tttttttgct
      481 tcgagaacgt gatttagatg aattaccgga atttctgcat acaccattta agccaatcct
      541 atcagagcta ttggaatttt atgatgcaga aaattgggca aaacgattta tcactaacga
      601 ttccactatt tatgagcaat acgaatatga cctctcctct gttgaaaaca aataaaaaac
      661 ctacattttg aagagattat gtactgaaac aaacaagcac aattcttatg gcatcatgag
      721 agttgtgttt tctttttgat cttttattgg gcttaaaaaa agagaaccag ctgctgattc
      781 agctgattct catcagagag ccttttattc tgcgccaagg attccttctt ttgcatggac
      841 taattgggtc aaaaaggcac aatatggtgt agcaatatca tatttttgtc cttttcgcca
      901 aatggcacca ttgatgtagt cgatttcagt caaacgatgg tttttgatca ggtcttggta
      961 catagatggg taatgcaacc cgatgttgtc aggatcataa gtagaagcaa tatgttggta
     1021 cacttcttct tgatctagta tgattccttc ttttgctgcg atagaagcaa attcagatac
     1081 gattgttcga accatgctct ctgccgcttt ttgcgcgcct agttcagcaa tattacaatc
     1141 caaaatcgtg cataaaccat tcaatgttcc atttacacaa gcttttcgcc aaatggaata
     1201 acgaacgtta tggctataga tcggatttaa atttgcttct tgaaagactt ctacaacttt
     1261 ttttgtaaat gcttcgcctt ctggttccaa gttctctagt tcgatttctc catcacctaa
     1321 caatgtgact tttccgggac cagctagacc agctgtccac atcgtgatcc caaataagat
     1381 atttttcttt ggcacatatt tttccagtac atcttcatga cctaatccat ttagtaaaca
     1441 caaaacataa gtattgtcag taataatgga ttggatcgaa caaaacatat catctaattg
     1501 attggctttt gttaatgcta cgatcaagtc cacgtgttcg ttgctttcga tgatttcttc
     1561 tggtgaatag attggcagtt ttgcgaccac ttctttaccg ttaaagtcag caatcaaccc
     1621 atttgtacga atggcttcaa tgtgagcagg ccaacgatcg atcaagagga cctcatttcc
     1681 gctttgatgt agcatcaatc cgattcggct gcccattgct ccggccccag ctattgcgat
     1741 tttcatgttt tttctctcct ttattcaatg agttcccttt tagtatatag aaaaatggaa
     1801 gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt gtcttactta agaagaaagt
     1861 gagggataca gatggatatt ttggcggcaa ttgattcatt taaaggaagt gcttcttcat
     1921 ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg taaaggaaga aaaatcaatg
     1981 tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg ctcaggggaa
     2041 catggcgtac cattgatggg atggatctct tattccgtca gcgaaaaatc agctatttat
     2101 tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc tgcacaagca ttggggttag
     2161 atctacttga taaaccaacg gatcaaacta tccgacttgc ttccagttat gccttaggag
     2221 aacttattaa agatgcactg cttcaagaag tagaacaaat tattgtcact ttaggaggaa
     2281 gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct ttagaagggt
     2341 acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga agaaacgttc
     2401 ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat acaggtcaac
     2461 aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg ctagctttct
     2521 tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag gtggatttag
     2581 atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt ttgttgggag
     2641 cgaagatggt ttctggtttc gatttagtca gccaaatcac tggattggaa aaaaagatcg
     2701 aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg atccacggaa
     2761 aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt
     2821 gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt gtcttaagta
     2881 ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca ttagaaaaaa
     2941 caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag tagttgacaa
     3001 aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat
     3061 tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggaa
     3121 gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct ttttgtgtgt
     3181 atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag
     3241 gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg acagccaagg
     3301 cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac taggacttat
     3361 cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca aactacctta
     3421 tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga caaaagataa
     3481 agaacgttta acctattacg tgttaaatga tattacggat tttgagatat gggaaatcga
     3541 aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact
     3601 atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg acactcattt
     3661 cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac aagtagcggc
     3721 ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg gatttatcga
     3781 tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa
     3841 tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga
     3901 aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc
     3961 tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata ctgaatctag
     4021 acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac gatccaaagc
     4081 aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa aagatatcga
     4141 atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac ttttaagcgt
     4201 agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt ttacaatcga
     4261 gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat ccattatcca
     4321 ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc caaaacatct
     4381 gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg cggaatttgc
     4441 agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc ttgggatgga
     4501 aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga aaaaagtgga
     4561 attggctaaa tctttatcac agccagctga actatataca tgggatgaac cattgaatta
     4621 tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga aacctgccat
     4681 gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga ttatttctct
     4741 tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg ggaactttgc
     4801 caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa cttggaacgc
     4861 acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac agatgcaggc
     4921 acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa tcatccattc
     4981 ccctcttcaa cgagcaagag atacagcaca agcagttgct gatcgtctca gcttgccgct
     5041 gaccgttgat gaacgattgg tagaaatgga ttttggggat tatgatggac tgccaagcaa
     5101 agatgagaac tttcaaaaag cacgtttagc gttcgctgta cgattcccaa atggggagtc
     5161 agtcttagat gtgtatgcac ggatcgttcc tttattgaaa gaatgcatcg aagacgaaga
     5221 aaatgtctat ctgttagttt gccataatgc cttgataaga gtgatcaatg cttattttca
     5281 tccgatgcct aatgaggggt tctttacatt catggtagat aatacagaat tggtttctta
     5341 tgagtaatta tgtttttgtg ttatttaaat aaaatatttc aaggcactgc ttttgcttag
     5401 cagtgccttt tttaggttat gacaattctt ttgataatat aaactcaacc tttagaaatg
     5461 aaatttaatt attttaataa aagtattgaa attttgtttc tttagatata tactctagtc
     5521 atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt caatatttat
     5581 gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat ccaatggctt
     5641 tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat atcaaaagcg
     5701 attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg tcgatatttc
     5761 aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt tgaaagcaat
     5821 tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg ccgactggtc
     5881 acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata tcgatgaatt
     5941 gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt atccacgtcc
     6001 cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca aaattcaagg
     6061 tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac tatatcaagg
     6121 attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc tagatctatt
     6181 agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa acgttcagga
     6241 gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac ctgtagatga
     6301 agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg caagagatgt
     6361 catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa gaaatacagc
     6421 tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct atatgggtga
     6481 gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt
     6541 tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact atcaatttat
     6601 aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac aacggaacgc
     6661 agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt tcaactaatg
     6721 aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag tatcgaacca
     6781 gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta tatgggagcc
     6841 ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac attcggtgta
     6901 gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac tgtagctgtc
     6961 gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct gaatttgaca
     7021 aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt gatcggtgga
     7081 ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa taaacaagcc
     7141 gaaatcagta aacacgcgga tttcccaatc gaagtcgatg caggtccaga atttttgaca
     7201 ggctctactc gattgaagtc aggaacagca caaaaactta tcttgaatat gatttctact
     7261 atcagtatga ttggtatagg taaagtattc aataacttga tggtcgatgt caaaccaaca
     7321 aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc aagcgacaga agctgattac
     7381 gaaacagctg aaaagtactt tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg
     7441 attttgacaa atagtgataa agaggaagct caagcaaaat tagctgaagc aaacggattt
     7501 gtaaaaggga ccttatgagg gggaaaagac catggcaaaa aacgaactga ctttggaaga
     7561 actggcaaaa caaatttata aaggggcagg cggcatgggc aatgtagaaa gcattgtcca
     7621 ctgcatgact cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg tctctgaatt
     7681 aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg
     7741 acctgggaaa gtgaacaaag tagcacagca aatggttgat ttggcaggcg tgaaattagg
     7801 agaaaaactt tcttctgtaa gtacagctac agcaaattca tcagcatctg gaaaagaccg
     7861 tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa
     7921 aatcaaatcg gtattaaaag acatttctaa tatctttgtg ccaatgattc ctgcatttgt
     7981 cggtactggg atcgtggcag ggattgccgc agttttagca aacttagtca cagccggaac
     8041 acttgatgca gcaacatggc aacaatatat cgatgtgatg aacatcttga aaaacgggat
     8101 gtttagctac ctagttatct atacaggtat caatgctgca caagtttttg gtgcaacacc
     8161 aactcttggt ggagttatcg gggccgttgt gatgctgaca gggatgaatc cggaagcacc
     8221 actgaaaaac ttatttactg gtgaagctct tgcagctgga caaggtggga tcctaggtgt
     8281 catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga
     8341 tgcggtagat atcatcgtta caccaactct ttcattgatt gccatcggtc tgattgaaat
     8401 tttcttcatc atgcctttag caggtttcat ttctgatggc atggtcggtg ggatcaactg
     8461 ggtacttggc gttggtggag catttgccgg cttcgttcta ggcacattat tcttgccgat
     8521 ggttatgttc ggactgcatc aaattttgac tccaatccat gtgcaaatga ttgatgaaac
     8581 tggtcgtacg ctattgcttc caattcttgc gatggctggt gcaggacaag tgggtgcagc
     8641 attggcatta tggattcgct gcaaaaaaga taaagaatta acagaaatga tcaaaggagc
     8701 tttaccagtc gggatcctag gaatcggcga accattgatt tacggagtaa cattacctct
     8761 aggacgccca tttattactg cttgtatcgg tggaggtatc ggcggggcag tgatcggaat
     8821 gatcggaaat gtcggggcta tcgctattgg accttcagga gccgctttga tcccattgat
     8881 ctctgacggt aaatggttag gctacatcct tggattattg gcagcttatg ctggtggctt
     8941 cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag aattagcgac
     9001 tgaaacagcc acagtaaatg aagtgcctga aaaagtagta cctcaaactt cagctgaaag
     9061 tgtgactatc ctctcatcag ttgctgacgg aacagttgtt tctttagata aagcaagtga
     9121 tccagttttt gcacaaaaga tgatgggtga aggctacttt gtagaaccag aaaacggaca
     9181 tatctattct ccagttactg gggtagtcag ctctgtcttc ccaacgaaac acgctattgg
     9241 gatcacaacg ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca cagttgactt
     9301 aggcggtacg ccatttgatg tgaaagtaac agaagggcag caagtgacac ctgatacgct
     9361 tgtagcagaa gctgacttag aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt
     9421 cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa
     9481 agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat agaaacaata gaaacgaaag
     9541 aagaaactga aatctatttt tcagcttctt cttttttatt atttattgtt ttctgctaaa
     9601 atctgataaa ttcgtgtcac atcagatggt gttccccgta attcataatc tgccaagaaa
     9661 ggaaagccaa atgtttgtgc atattgtttg gctgttagac aatactgatt attgaagttt
     9721 ttatttccgc taccgactac accaagacaa agtttatgat tgtcttcatg ttccagatat
     9781 tcacgcatcg tttcggtcaa gatttctgta tcaccgttat ctaaaccatt tccgccgtct
     9841 aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc actattttca
     9901 tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat tttttcagca
     9961 tattctgcta gatgtttggc aaaggcacgc gtatttcctg aaatcgatat atataaaatg
    10021 ttcatggatc atcctccaat tctttccttt tagtatagca tcttttttaa gttttttggt
    10081 ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa
    10141 ttatatttct attaaagttt ttattaatta agtaaaaata aaaaaggttt gttatacttt
    10201 ttgataaaag cggttttatt ttggaggatg cttacatgga tacagagcaa cagaaaaaag
    10261 gaattggttt aattccactt gctgccttag taattggttc agcgattgga ggtggcgtat
    10321 ttggtattat gacggacatc tccggatcgg ctggcggccc agctattttc agttgggtac
    10381 tcgtaggaac agcaatgttg atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc
    10441 cagatttaaa aggcgggatc tttagttacg cagaagaagg ttttggccgt ttcgctggtt
    10501 ttatcagcgg ttggggttac tggctcacat gctggttagg caacgtagct tttgcaacat
    10561 tgttgatgag cgcattagga tatttcttcc cagtatttga aggtgggcaa aatctggcat
    10621 cgatcgtgat gagtacagtc tttttatggg gatatgcatg gctagtgaat cggggagtcg
    10681 aaaatgctag tttcgtcaat gctatcgtca cattctgtaa attagtgcca ttatttttat
    10741 ttattgtagt agctatcagc acgttttcga tccaacaatt cattgataac ttttctagcg
    10801 acattatatt gaatgcgaat ggacagccga tcccgttttc cagtcaagta ttgagttgca
    10861 tcatggtgat gctttgggta tttgttggta ttgaaggagc ctctgtcgta ggttcacgag
    10921 caaaaaaaca atcagatgtt gggaaagcaa cgattctagg gatattcggc ttgattatta
    10981 tttacgtctt ggtttccatg cttccatatg ggacaatgac gatgaatcag ctgcaaacga
    11041 tcaaggcgca gcctgctatg ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag
    11101 tcgtgatcaa cggcggtttg atcatttctt tagttggtgt ttggctctca tggacgatct
    11161 taccgattga aacaatggga aacatggctg aagacggact tttgcctaaa gcatggggaa
    11221 aactcaacaa aaatggggca ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg
    11281 tctttttact ttcattatta ttcactgaca gtgcttataa ctttgcttac acacttggta
    11341 cagcagctat tttcttcacg tggcttttct taggattgta ccaaatgaaa ctttcttatc
    11401 agcggaaaga atgggtccaa tttttagtag ggcttttagc aagtgccttt caagtatggg
    11461 ctatgctagt cgtagccttt aatgaagtaa tgcttgttct tgttcttttc ttgccaggca
    11521 tttattttta cctcaaagca cgtaaggagc aaaacgagcc tgtacaacat atccacttag
    11581 ataagatttt acttataggt gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca
    11641 ctgggatgct tcacgtatag aagtataaaa gaagtataaa gagaggttgt gagcctgtcg
    11701 tttattcaaa aacatactag gaaaggagct gtgacacaaa tgttgtcaca gctccttttt
    11761 cagaataaac ggtgtttaag agacagccaa tcggcaataa ggctgaacgt ctctttcaca
    11821 gcctcttttt tgatgagtca tcatacattt tttcgcttaa atacgagata tcctaatgct
    11881 aagaacaaaa cgatgtaagc taaattaccg aatagcattt gattagtaga aagatgagtc
    11941 aattgcttca tgatctcttc gtttccaatt tggttttgca gattcaacat actgatcgga
    12001 ttccatttga tccactccca tttttggatg agtgcaataa ggataccaga tatgattgac
    12061 gaagcaaaat aaaagacgat tcctgctgag atggatgcgc cggaactatt gataaagcaa
    12121 gcaatcatca acactaaact taaaatcagc cataaaccga tataactacc caaagtataa
    12181 gtgaacaagc tttggatcag tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa
    12241 gggaaaagaa tcagtttcat caacacagtg acaataatag ctaaaaggta taaataaaca
    12301 gaataaataa ctaatgtgat ccatttgctt actaatattt ctcctcgaga atactttcga
    12361 taaaggagat ttttcaatgt accatattgt gcttccatcg aaatgatcgt gcttgcagct
    12421 gcaatcatga taaagacaat ccaagatgtg gcactaaaaa gctgagcagt catttcaact
    12481 ggatcaatga tcgtcgtgta ttttttggct agaagtgctg ttcctatgag caaaacaact
    12541 aatagaacag ataaaacagc agttgacttt ttcttgatca atttaaataa ttcttgtttt
    12601 acaagcaatg tcatttttct tcttccccct ttactgaatc cagaatttcc aatagagaag
    12661 attccaaatc attcgtcaga tgctggacgt ctgtcacatg gatattttgt gcagacaata
    12721 gctggatcac ggttcctaat aattcttcgt tttcttttaa aagcttgatt ttatcaggat
    12781 cttccaaagt atatccagct tctgtcaaga tcgttcgagc agctagatca tttgttgtag
    12841 agataacgat gaaatcttgc gctgtttggt tcaattcagc cattgtacat tgtttgatga
    12901 tctttccttg attgatcagg atcagctcat cgaccatttt ttcaagctcg ctgagtaagt
    12961 gactggagat caagaaagag gtgccctgag tagccaagtc ggtaatgatt tttcgcacat
    13021 ctcttgttgc ttgtggatcc aaaccgttca tcggctcatc taaaataacg atagacggat
    13081 ggttcagtaa ggcaagagca attcccaatt tttgtttcat tcccaacgag taggatttcg
    13141 cttttttgtg aatgtacttt tccatcttga gctgattcac gatagtgtcg atagcagata
    13201 catcgttggt ttcagagaat aacttcagat gatcatagcc tgttaagaat ggatagatac
    13261 ccgggtattc gatcaatgct cctacttgtt ccaacccttt atgactagag ggagaaatct
    13321 cattttcacc aatacagatt gttccatctg agtaatggat cagtcctaaa atagctttca
    13381 tgatcgttgt ttttccagcg ccattaggac ctactaatcc aacgattttg ccaggggata
    13441 agctaaaact gacatcgctg agggcttgtt tgtgaccaaa gttcttggat aattcttgga
    13501 ccgaaagact tttcaaaaat attcactcct tttctataga tagtacaagc atacacctga
    13561 tgagtggaga agtcgctagg tcattggact tatttcttat tttttcataa aaaaattgaa
    13621 tattaaataa aaaggcttat tttttctgat cagtacttat tccaagtgtc tagtggccgc
    13681 tagtaatctt gatgccaatg atactgatga gtagcatccc cacaaagaac caagtggaaa
    13741 gattcagctt atcatgaaac aagaccacac caattatgac cgaaccaact gcgccaatcc
    13801 ctgtccagat aggataagag agactcatcg gcaaactttt gattgccttt gacaaaaagt
    13861 aaaaactagc aatcatgcca atcactgtgt aaaaagaata attgatttta gaaaaaccat
    13921 cactccattt catcatcgtt gaccagaata cttccaaaat tcctgctatg attaattcca
    13981 tccatgccat acttacattc ctccttaact ataaaaaaat acacaaaaag aggaaaatca
    14041 tcccttcaaa gacctaatgg aatgatcatc ctctttatcc tacccggtgg cagattgtat
    14101 atctttttct gtagtatagc acacctatcc agatatcggc aatctcatac tttttagtaa
    14161 agtgtaccaa gcactgacgg atatatcgtt agcggaaaag aagatgaatg ctttgcaatt
    14221 tcatagaaag aagttgttat ttattaatca tttaaaaaaa acagtaacta gaccttattt
    14281 ctatatatta tcgggcaata aattaaaaaa agctttacct tacaataagt aagataattt
    14341 ttgtgataaa aaaataacga aaaatattta aaaatagtgt ctatttcgtg agaaaacaac
    14401 tatatttttt atgtgttttg ctttataatt aaaaaaatat tgtcatttta tggaggaaaa
    14461 tatatggaaa attatcttag ccttttaggc gtcttgattg ttattctcgg gtttgcattt
    14521 aaattagact cgatattgat cgtaatggtt gctttagttg ttacagcttt gactggcggt
    14581 cttggcattc aaggaatgtt agaactttta ggtacaagtt ttgtaaacaa tcgcgggatg
    14641 gcgattttta tcatcattat gttggcaaca ggaacgcttg aaagaaatgg tctgaaagaa
    14701 tcagcggctt ccttgatcaa gcgattcaag aaagtatctg ctggtatgat tgtagatatt
    14761 tatgggatct ttcgaatgat ctttgcggct tttaacgtga gttttggtgg agttgctgga
    14821 tttgttcgtc caatcatttt accgatggca ttggggacca tcgaatcaaa agggttagaa
    14881 gtccatccta agcatgaaga agaactaaaa ggtatggctt cagctatgga aaatgtctgc
    14941 tggttctttg gccaagtact attcatcgga ggagcagggg cgttgctagt ccaatcaaca
    15001 ctgaaagatc taggatatga cgtaacattg ggacagcttg ctttagtgga agttccagtg
    15061 gctatcacgg ctttagttgt ggcaagtgtc tacttctatc ttaaagaaaa gaaattagcg
    15121 aagaaatatt atccaggaaa ggggcaataa gatggacttt ttt
//
LOCUS       DBNIBO010000300         6608 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.137, whole genome shotgun sequence.
ACCESSION   DBNIBO010000300 DBNIBO010000000
VERSION     DBNIBO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.137"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            241..1209
                     /locus_tag="AC1Y77_002857"
     CDS             241..1209
                     /locus_tag="AC1Y77_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HIQ1576356.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1202..2233
                     /gene="vanA"
                     /locus_tag="AC1Y77_002858"
     CDS             1202..2233
                     /gene="vanA"
                     /locus_tag="AC1Y77_002858"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HIQ1576357.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2239..2847
                     /locus_tag="AC1Y77_002859"
     CDS             2239..2847
                     /locus_tag="AC1Y77_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HIQ1576358.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3275..4186
                     /gene="vanY"
                     /locus_tag="AC1Y77_002860"
     CDS             3275..4186
                     /gene="vanY"
                     /locus_tag="AC1Y77_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HIQ1576359.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4339..4824
                     /gene="vanZ-A"
                     /locus_tag="AC1Y77_002861"
     CDS             4339..4824
                     /gene="vanZ-A"
                     /locus_tag="AC1Y77_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HIQ1576360.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5111..5440)
                     /locus_tag="AC1Y77_002862"
     CDS             complement(5111..5440)
                     /locus_tag="AC1Y77_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIQ1576361.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5745..6074
                     /locus_tag="AC1Y77_002863"
     CDS             5745..6074
                     /locus_tag="AC1Y77_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIQ1576362.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6064..6351
                     /locus_tag="AC1Y77_002864"
     CDS             6064..6351
                     /locus_tag="AC1Y77_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIQ1576363.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
BASE COUNT         2180 a         1095 c         1366 g         1967 t
ORIGIN      
        1 tggtgtcatc caccaacatc aaaaagtata gagccataat tttttaggaa aatctcaagg
       61 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
      121 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
      181 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
      241 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
      301 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
      361 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
      421 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
      481 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
      541 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
      601 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
      661 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
      721 gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
      781 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
      841 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
      901 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
      961 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
     1021 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
     1081 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
     1141 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
     1201 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
     1261 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
     1321 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
     1381 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
     1441 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
     1501 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
     1561 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
     1621 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
     1681 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
     1741 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
     1801 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
     1861 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
     1921 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1981 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     2041 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     2101 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     2161 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     2221 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     2281 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     2341 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     2401 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     2461 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     2521 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     2581 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     2641 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     2701 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     2761 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     2821 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     2881 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     2941 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     3001 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     3061 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     3121 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     3181 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     3241 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     3301 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     3361 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     3421 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     3481 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     3541 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     3601 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     3661 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     3721 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     3781 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     3841 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     3901 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     3961 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     4021 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     4081 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     4141 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     4201 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     4261 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     4321 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     4381 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     4441 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     4501 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     4561 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     4621 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     4681 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     4741 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     4801 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     4861 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     4921 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca
     4981 aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta
     5041 acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat agatagattt
     5101 tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct aaagagtaat
     5161 aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa aaacgtaaat
     5221 ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag acgcacattt
     5281 ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg aaacatttcc
     5341 ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt ccatttactt
     5401 tttctttatg tacgacagtc acttggcaaa cttttttcat tcatctcacc tcacattcaa
     5461 acgattattt gaatgaagta tacctatttg atcactcttt tgtcaatcta ccttaattcc
     5521 aatacactat tataagcaat ttcatgtatt gaagtaaact tacttgcaag tgcgtttttg
     5581 attccaagtt aaaaacatat tattattcta cataatttcc taatcaaata aaactccttt
     5641 gaaaattgtt ctaaaggagt tttattattt tgtatttcta ctaaaaatag cgtacaatgt
     5701 aattactaag taattacgaa atgtaaaata atggaggaaa taatatgatt acaaaaacaa
     5761 gaaaacaagg aaattctatt atgttaacgg tccctaaaga attcgatgtg cccaatggtg
     5821 ttgaagtaga agctaagctt gtagagaatg gtatccttta tgagtttgta gagcctaaaa
     5881 aagaattttt tgattttagt gaagatattt tggctgatat tctttcagaa ggttatagca
     5941 aacaagagat cttaaaagaa tttagaaata gaaaaaatgc gttaacttct gcatttaaat
     6001 ctattgctga agatactgtt gcaaattcta aaccgatgac aaaagaggag ttggcagcag
     6061 aaattggctt atgagattct accttctaaa cacgtaataa agtatctaaa aaaattaaaa
     6121 gagaaaacat taaaagaaca atttttaacg ataatttatg atgagatagc agttcgtcct
     6181 cattcgggag aacaaaaaac gggtgattta tcaggaattt gggctattgg attcaagtat
     6241 gcaggtacga catatcgcgt tgcatatgaa attaaagata atgcagtgat acctaactta
     6301 ctatgtggaa cgcatgaaaa tttttatgaa caattaaaaa agattaggta agaacccaat
     6361 gtttcaactt gagaggatag acgacacttt aaaagataat ttttaacagc tgagagttgt
     6421 aataccctat aattttaaac cactaaccta tatatttatg aaaaatagaa aaaattaaaa
     6481 cgcattttaa gcctctttca aattaaaagc ttcatcctta tgcacaaaga gggcttaaaa
     6541 tgcgttattt tatattgttt tttagtaatt cttaattgtc aaattaagtt agacagatca
     6601 tcaatact
//
LOCUS       DBNIBO010000301         5801 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.148, whole genome shotgun sequence.
ACCESSION   DBNIBO010000301 DBNIBO010000000
VERSION     DBNIBO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.148"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            321..785
                     /locus_tag="AC1Y77_002865"
     CDS             321..785
                     /locus_tag="AC1Y77_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIQ1576364.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            769..1398
                     /locus_tag="AC1Y77_002866"
     CDS             769..1398
                     /locus_tag="AC1Y77_002866"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIQ1576365.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1752..3230
                     /gene="msr(C)"
                     /locus_tag="AC1Y77_002867"
     CDS             1752..3230
                     /gene="msr(C)"
                     /locus_tag="AC1Y77_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HIQ1576366.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3277..3723
                     /locus_tag="AC1Y77_002868"
     CDS             3277..3723
                     /locus_tag="AC1Y77_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIQ1576367.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3922..4437)
                     /locus_tag="AC1Y77_002869"
     CDS             complement(3922..4437)
                     /locus_tag="AC1Y77_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIQ1576368.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4554..5755
                     /locus_tag="AC1Y77_002870"
                     /pseudo
     CDS             4554..5755
                     /locus_tag="AC1Y77_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1792 a          969 c         1230 g         1810 t
ORIGIN      
        1 ctgtttaagc atactgatcc aatttctaat attcgacggg attcttgcaa aaggcgattt
       61 cggaaatttt tggcggtgta aaattttcta ctgcagaaga gctatcttct atagtttgat
      121 aagaatcaat aatcttggtt ttagcgttgt gctttcttta tcattttttc cgttcattct
      181 acaaaaaatt tcttttctct atacttttct attgagagcg ttttttttat tacacagctg
      241 attctttgac ttttttcttt ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa
      301 ttattagaaa ggaggaaatt atgtctttag aagagattag tcacctttta tatcaaatca
      361 aattagccga tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata agtcttacta
      421 gatacgagat catgatgaga ctgaaagaag aaaatccatt gctacaaagc agtttgcaag
      481 aaaagttgca gattgatcaa ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg
      541 gctatgtttc gcgtcagaga aatcctcaga acaaccggga agtgtatgta gcgctaacag
      601 aaaaagccca gaagaaaatc gagcattgtg aaatggacca taaagataca ggttccgtgc
      661 tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta gataaattga
      721 atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat ggaacaaaaa
      781 aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga tgaaacatac
      841 aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc accttcttct
      901 gtcaatatgc aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg
      961 cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat ggtcatgatt
     1021 tttggcgata tgcaatgtta cgaatatggc gaagaaatct ataaccaagc ttacgaatca
     1081 gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc ttattacaag
     1141 agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt agcggctatg
     1201 caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg tggattcgaa
     1261 gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc agttattatt
     1321 ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc accagaaaag
     1381 atcactacgt tcgaataaaa ggttttcagt tactattcag tagctattgc tttttttgaa
     1441 tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg atgaaattac
     1501 gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa caacaatttc
     1561 atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc tatgacagat
     1621 ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt
     1681 gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga
     1741 gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca
     1801 accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca
     1861 taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc
     1921 ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc
     1981 tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag
     2041 aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc
     2101 tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa
     2161 gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc
     2221 atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa
     2281 tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga
     2341 ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc
     2401 aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac
     2461 aaatcagacc ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc
     2521 aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc
     2581 agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc
     2641 ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg
     2701 attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga
     2761 caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa
     2821 gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt
     2881 ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag
     2941 ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc
     3001 gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga
     3061 ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg
     3121 gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt
     3181 atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg
     3241 atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa aaaagaagga
     3301 ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc gtataattta
     3361 tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc tggattcgtg
     3421 gtggctttca ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa
     3481 ctaccagtaa atccagaaag caaggtccaa atgatcttaa ctatttcatg cttgatggtt
     3541 ttaggaatgg tgacatgtat gtctttattc ggtgtgatca tggaacaagg aatacctgat
     3601 caattattct ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg
     3661 cagttgatca tcgttggccc gctttcaaga agtgtgctaa aagcaattca aagtaatgaa
     3721 tgatggaaca ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa
     3781 tgcagttcta gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt cggatcgctt
     3841 tttcttttcc aactgtattg ctcttttatt gaagataatt tgctcttcaa tagatatttc
     3901 tagaaaggtt ctgtttatcc cttactcaaa aaactgctgc atttgttctc ttacttgctg
     3961 acaccattgg agttcctgct gacaaagcat aattctgcgc tggatcacaa cataacgtcc
     4021 aaattcttgt ttcttgtcta gcgagaggtc attcaatttt ttttgatttt tttgcatgat
     4081 tttgttcagc tgttgttctc tagtgaatag cagtccagta gctgttgttt tatcgagcgt
     4141 cgaaaaagca tagatttttg ctagaaattc atctttttgc gtaggagcgg gagtttcctc
     4201 tttgatccat tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga tctttttttg
     4261 agagtgctcg tctttgtcca aaacggtcac ataaccctgc tggtctaact tggcaagcgt
     4321 tgtatagatc tgactgtgat gggcttccca aaaaattgtg atcagctgct tcaattcata
     4381 cccagaacgt ggtttggata aaagcacgca taataatata taggctaaag tattcataaa
     4441 aatcctacct ctcgtcttat tttctttatt atatcacagt aaagtttcca cgttttacat
     4501 gtgcatattt acatgtaaaa taaatcatgt atgatgctaa tagagagaaa gggatggaaa
     4561 tattggagaa tcaaatagat atacaagaac aaacaaatga agaaacaaag gggaaaagtt
     4621 cagcgctagt tgtagcagct acctgtatga tcgcttttat gggagtgggt ctagtagacc
     4681 caatcttaaa aacgattgct gcacagttaa acgctacgcc agctgaaacg accctgttat
     4741 ttactagtta catgttagtc actggtgtgg tgatgctatt tacaggtttc ttatctagtc
     4801 ggatcggttt gaaaaaacat tgatgacagg attgtttatc atcgttgtat tcgctggtct
     4861 tggcggatta tctgggaata tcggaatgct gattgggtta cgtgccggct ggggtgtcgg
     4921 gaatgcttta tttgtctcta ctgctttagc aacgatcgtc agtattttag ttggtaacac
     4981 agaaaaagca atcatgatgt atgaagcagc acttggctgc ggaatggctg ttggccctct
     5041 agtcggtggg gtcttgggca gtggttcatg gcgttatcca ttttttgggg tagcattttt
     5101 gatgttttgc ggttttatag ccctagcggt acttttacct gaaactgaga aaccaaagag
     5161 aaaagtaggc ctttgggaag gtgtcaaagc gttaagcaat cataaacttc gttcagttgg
     5221 actaatcgca ttgttatata atttcggatt ctttacttta cttgcgtatg cgccattttt
     5281 attgattggc tattctgaat tagaagtcgg ttttgtattt tttggctggg gtgtgcttct
     5341 ggctattgct tcgattttcg ttgcaccggc tcttgagcgg aagtttacga cttatcggac
     5401 aattttagca gcatttgtct tattcattat ttgtttgctg ttattaggag ctggaacttc
     5461 tatgccagtc cttgtaccga tcagcattgt gttagcagga tttttccaag gaattatcaa
     5521 tacactgttg acgacgattg ctatggaaat accaggtctg caacgaaacg ttgcttcgtc
     5581 cagctatagt tttgtccgct ttttcggtgg agctttagca ccatttattg caggtgtgat
     5641 tggtgaacat attggtgaaa gctatgcgtt ttattttgca ggtgccattg ttcttatcag
     5701 tgtatgttgt atcatttatc accgtaatta ttttgctaca aatgaagaag aataagaaat
     5761 cagaggttgt gaaaaagctg tcctgcatca agtcataaga a
//
LOCUS       DBNIBO010000302         4985 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.162, whole genome shotgun sequence.
ACCESSION   DBNIBO010000302 DBNIBO010000000
VERSION     DBNIBO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.162"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            3..188
                     /locus_tag="AC1Y77_002871"
     CDS             3..188
                     /locus_tag="AC1Y77_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIQ1576369.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="AC1Y77_002872"
     CDS             203..1072
                     /locus_tag="AC1Y77_002872"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIQ1576370.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="AC1Y77_002873"
     CDS             1053..1787
                     /locus_tag="AC1Y77_002873"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIQ1576371.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="AC1Y77_002874"
     CDS             1820..2728
                     /locus_tag="AC1Y77_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HIQ1576372.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3267
                     /gene="sat4"
                     /locus_tag="AC1Y77_002875"
     CDS             2725..3267
                     /gene="sat4"
                     /locus_tag="AC1Y77_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HIQ1576373.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3360..4154
                     /locus_tag="AC1Y77_002876"
                     /note="aph(3')-IIIa"
     CDS             3360..4154
                     /locus_tag="AC1Y77_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HIQ1576374.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4430..4972
                     /locus_tag="AC1Y77_002877"
     CDS             4430..4972
                     /locus_tag="AC1Y77_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIQ1576375.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         1710 a          750 c         1134 g         1391 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     3001 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     3061 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     3121 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     3181 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     3241 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     3301 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     3361 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     3421 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     3481 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     3541 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     3601 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     3661 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     3721 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgattag
     3781 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     3841 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     3901 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     3961 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     4021 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     4081 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     4141 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     4201 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     4261 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     4321 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     4381 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     4441 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     4501 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     4561 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     4621 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     4681 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     4741 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     4801 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     4861 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     4921 agaaccggaa agcagaatga tagagccggt tctgttgcaa agttttaaat aaagaataaa
     4981 atccc
//
LOCUS       DBNIBO010000303         3970 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.184, whole genome shotgun sequence.
ACCESSION   DBNIBO010000303 DBNIBO010000000
VERSION     DBNIBO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.184"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002878"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HIQ1576376.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="AC1Y77_002879"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="AC1Y77_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HIQ1576377.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1255 a          613 c          817 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DBNIBO010000304         3970 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.185, whole genome shotgun sequence.
ACCESSION   DBNIBO010000304 DBNIBO010000000
VERSION     DBNIBO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.185"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002880"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="AC1Y77_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HIQ1576378.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="AC1Y77_002881"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="AC1Y77_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HIQ1576379.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1254 a          613 c          818 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DBNIBO010000305         3289 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.198, whole genome shotgun sequence.
ACCESSION   DBNIBO010000305 DBNIBO010000000
VERSION     DBNIBO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.198"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            288..785
                     /gene="dfrG"
                     /locus_tag="AC1Y77_002882"
     CDS             288..785
                     /gene="dfrG"
                     /locus_tag="AC1Y77_002882"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HIQ1576380.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(857..2809)
                     /locus_tag="AC1Y77_002883"
     CDS             complement(857..2809)
                     /locus_tag="AC1Y77_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1576381.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2816..3112)
                     /locus_tag="AC1Y77_002884"
     CDS             complement(2816..3112)
                     /locus_tag="AC1Y77_002884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIQ1576382.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1020 a          567 c          541 g         1161 t
ORIGIN      
        1 gtatatatgc aagtaagtag ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa
       61 tggactactt ttttcatttt aacttacata ccaaattaca tacaaaatga tttataaaat
      121 gcaattgaaa atgataattg aatgaaaatt tgtgattttg tggtattatg ataatataac
      181 ttatagaaaa aattgcttat tcaactaacg ggcaggataa tgagaaaagg ttttttgata
      241 gaaataagaa attagatggt aaattagtta aaaaggctgt gttataaatg aaagtttctt
      301 tgattgctgc gatggataag aatagagtga ttggcaaaga gaatgacatt ccttggagga
      361 ttcccaagga ctgggaatat gttaaaaata ctacaaaggg acatccgata atattaggta
      421 ggaagaacct tgaatcaatc ggaagagcct tacctgacag aagaaatatt attctgacga
      481 gagataaggg gtttaccttt aatggttgtg aaattgttca ttcaatagaa gatgtttttg
      541 agttatgtaa aaacgaagaa gaaattttta ttttcggagg agaacagatt tataatttgt
      601 ttttccctta tgttgagaaa atgtacatca caaaaataca tcatgaattc gaaggagata
      661 ctttttttcc agaagtgaat tatgaggaat ggaatgaggt atttgcccaa aaagggataa
      721 agaatgataa aaatccgtat aactactatt ttcatgtata tgaaagaaaa aacttattga
      781 gttaacgggt gcgttaattg aagaagaaca gtaaagataa agcacaagag cttgggatta
      841 ctccaagctc tttttgtcat ttgaaataaa ttatatcttt ccaatcaaat tgtttatctg
      901 taatccctaa tctttgagca ggtgtcaaaa cctttttatc cgctgattta aaagggcgac
      961 aaaagttgta gtaagttctt agaattgtaa tcgcaaattg agcatacttc ggattgaagt
     1021 tagcgtaaat atagctcttc ttatcaccac gagctgttgt taatggtctt tcaagagatg
     1081 atatataacg tcttatctgt tgaataaagc tattggtcga atggtcatta acatttaata
     1141 gcatctttgc gatttcgttt ggttccagag cagataaatc cgttctacaa tcaactgaat
     1201 aaaatccttt atccttagtg gctaatggat gtttgatagg gttatcggca tatttgcgat
     1261 atctttctcc atctttacta atagcctctt cgtggaattg atgacttgtt agtaattcag
     1321 ttagataatg agatgccaaa gtccgtagaa actttgtttt gaatcctctg atatctcccc
     1381 aatcgagcag ttctgctttt gcttgtccga attcttttaa gcattgtttt ctgcttttcg
     1441 tcttgttaac ttggcaaata aaatgatgtg catcgctcaa ccttaattct ttcgtgaata
     1501 ctctataaaa ggcgtttcta atagagaagt catcatcact aatcattcgc cattcgtcgg
     1561 cattaaccat ttgtttaatt aaccaatagt gagcagttgt agtataagga gcatcaacat
     1621 ttaaaccatc gacaaatcta ctcctattag taattttccc taattcgtga agataagcat
     1681 tcctattttc gctatcgtta gcggatggtt cttgtggata ataagaccaa tctaacctat
     1741 catttttacg actaaaagta ttgagatggt cttctttgaa ttttcttgta tcttcattta
     1801 attcatccat tgaaatattc caatcatagg caacatcaga acggaacaca tatcttgata
     1861 agacctcagc agatacaact atatacgttt gcatattaag gtcttcaaac cccgtatatt
     1921 tctttgaacc ttgtcctttc ttacggacgt tatttaggta gtagtgcatc ttgtccgtat
     1981 taagccacat ctcgttaaat ttctttgttt gaaggggttg tgtctcatat cgttctaaaa
     2041 actccaaaca acgcctatac agccactcca gcttatgata gtaggttcca actccaattc
     2101 ctaaaatatc acaagtacga ctgactgaaa cccttccaac aaccatctta gcgaacattg
     2161 gaagaatggt gttcttttgt tggtgataag tagtggtttc ttctcttttt ggtagaacat
     2221 tagtgaactt cttacacgcc ttacattgat agcgttgaga tttgccccta cttttccccc
     2281 ttttgtaaaa ttgcttcggc tcattgaatg gggttgattc ttcttccgag cagccttctt
     2341 tatgaaattg ataatctggc tcaacatctt ggatgctatt gatttctata aggcgtttaa
     2401 tttcttcaac aacagaccaa ttagataaag gggttactgt acagttttgc gatacacctc
     2461 tgccaatagg attgtcattg cagtacatac ctttatgacc tttatctttg gaactaccag
     2521 tcattttata tcgacttggt ttccctttta catctttgaa tttgtgctgt tctttcccaa
     2581 aattacaaca aaacggattg atgcagtaat tatattgaat gggatattca gaaccgttcc
     2641 atttaaaaga cgttggcaag aacaacattt tgttatacct tgttgcaaat cgttttggag
     2701 ttaataaatc atattgttta gccctatctt ttaattcttc gtttgaaatc ggaattttaa
     2761 catctacaat ttcatcttcc ttcgtggcta atcttttaag cttggtcatt gtctatcaac
     2821 ccactctatc tttgataccc attttttcaa caatccgctt attacttcgc ttgttggcaa
     2881 tccattcaat aaacttctta tcgtctaaaa tgttcaataa gaattcatcc actgcttcgc
     2941 tttcagtacg ctctgcatat tcagcgtatt gcttaactat cacccttact tgctctgata
     3001 tttcccaatc cacagactta gcgttcttat tttttggctc aagaaattgc ataggaaaat
     3061 gcaccccttt ttgcttagta aattgtaacc attttaacac accaatttgc attcctacaa
     3121 taattatttc acgttcgcat ttttatatac attttaactt acattttgtg gtgtaaatta
     3181 acaacaaacg ttgatttaac aataaaaaac gagtatcaaa ttcagttgca atttgatact
     3241 catttaggtt tacattttca ctttttgtac tccaaaactg aactactta
//
LOCUS       DBNIBO010000306         2517 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.212, whole genome shotgun sequence.
ACCESSION   DBNIBO010000306 DBNIBO010000000
VERSION     DBNIBO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.212"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..423
                     /locus_tag="AC1Y77_002885"
     CDS             <1..423
                     /locus_tag="AC1Y77_002885"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIQ1576383.1"
                     /translation="PYLIVDLKLCNRTTRITRSTQDLFELIDNIRDKKASLKSLKDTW
                     LDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAG
                     MNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            637..1332
                     /gene="vanR-A"
                     /locus_tag="AC1Y77_002886"
     CDS             637..1332
                     /gene="vanR-A"
                     /locus_tag="AC1Y77_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HIQ1576384.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1310..2464
                     /locus_tag="AC1Y77_002887"
     CDS             1310..2464
                     /locus_tag="AC1Y77_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HIQ1576385.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          866 a          449 c          529 g          673 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ctcgaatcac tcgtagtaca
       61 caagatctat ttgaattaat cgataacata cgagataaaa aggcaagttt aaaatcacta
      121 aaagatacat ggcttgattt atcagaagat aatccataca gccaattctt aattactgta
      181 atggctggtg ttaaccaatt agagcgagat cttattcgga tgagacaacg tgaagggatt
      241 gaattggcta agaaagaagg aaagtttaaa ggtcgattaa agaagtatca taaaaatcac
      301 gcaggaatga attatgcggt aaagctatat aaagaaggaa atatgactgt aaatcaaatt
      361 tgtgaaatta ctaatgtatc tagggcttca ttatacagga aattatcaga agtgaataat
      421 tagccattct gtattccgct aatgggcaat atttttaaag aagaaaagga aactataaaa
      481 tattaacagc ctcctagcga tgccgaaaag ccctttgata aaaaaagaat catcatctta
      541 agaaattctt agtcatttat tatgtaaatg cttataaatt cggccctata atctgataaa
      601 ttattaaggg caaacttatg tgaaagggtg ataactatga gcgataaaat acttattgtg
      661 gatgatgaac atgaaattgc cgatttggtt gaattatact taaaaaacga gaattatacg
      721 gttttcaaat actataccgc caaagaagca ttggaatgta tagacaagtc tgagattgac
      781 cttgccatat tggacatcat gcttcccggc acaagcggcc ttactatctg tcaaaaaata
      841 agggacaagc acacctatcc gattatcatg ctgaccggga aagatacaga ggtagataaa
      901 attacagggt taacaatcgg cgcggatgat tatataacga agccctttcg cccactggag
      961 ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa aattcagtgg agtaaaggag
     1021 cagaacgaaa atgttatcgt ccactccggc cttgtcatta atgttaacac ccatgagtgt
     1081 tatctgaacg agaagcagtt atcccttact cccaccgagt tttcaatact gcgaatcctc
     1141 tgtgaaaaca aggggaatgt ggttagctcc gagctgctat ttcatgagat atggggcgac
     1201 gaatatttca gcaagagcaa caacaccatc accgtgcata tccggcattt gcgcgaaaaa
     1261 atgaacgaca ccattgataa tccgaaatat ataaaaacgg tatggggggt tggttataaa
     1321 attgaaaaat aaaaaaaacg actattccaa actagaacga aaactttaca tgtatatcgt
     1381 tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt cgttcaatga tccgagggaa
     1441 acttggggat tggatcttaa gtattttgga aaacaaatat gacttaaatc acctggacgc
     1501 gatgaaatta tatcaatatt ccatacggaa caatatagat atctttattt atgtggcgat
     1561 tgtcattagt attcttattc tatgtcgcgt catgctttca aaattcgcaa aatactttga
     1621 cgagataaat accggcattg atgtacttat tcagaacgaa gataaacaaa ttgagctttc
     1681 tgcggaaatg gatgttatgg aacaaaagct caacacatta aaacggactc tggaaaagcg
     1741 agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac gttgttatgt acttggcgca
     1801 cgatattaaa acgcccctta catccattat cggttatttg agcctgcttg acgaggctcc
     1861 agacatgccg gtagatcaaa aggcaaagta tgtgcatatc acgttggaca aagcgtatcg
     1921 actcgaacag ctaatcgacg agttttttga gattacacgg tataacctac aaacgataac
     1981 gctaacaaaa acgcacatag acctatacta tatgctggtg cagatgaccg atgaatttta
     2041 tcctcagctt tccgcacatg gaaaacaggc ggttattcac gcccccgagg atctgaccgt
     2101 gtccggcgac cctgataaac tcgcgagagt ctttaacaac attttgaaaa acgccgctgc
     2161 atacagtgag gataacagca tcattgacat taccgcgggc ctctccgggg atgtggtgtc
     2221 aatcgaattc aagaacactg gaagcatccc aaaagataag ctagctgcca tatttgaaaa
     2281 gttctatagg ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac ttggattggc
     2341 gattgcaaaa gaaattattg ttcagcatgg agggcagatt tacgcggaaa gcaatgataa
     2401 ctatacgacg tttagggtag agcttccagc gatgccagac ttggttgata aaaggaggtc
     2461 ctaagagatg tatataattt tggctctttg tcaaatgatg ttggtaaaga gaaatct
//
LOCUS       DBNIBO010000307         1749 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.234, whole genome shotgun sequence.
ACCESSION   DBNIBO010000307 DBNIBO010000000
VERSION     DBNIBO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.234"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            complement(<1..583)
                     /locus_tag="AC1Y77_002888"
     CDS             complement(<1..583)
                     /locus_tag="AC1Y77_002888"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIQ1576386.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLG"
     gene            1136..1684
                     /gene="aac(6')"
                     /locus_tag="AC1Y77_002889"
     CDS             1136..1684
                     /gene="aac(6')"
                     /locus_tag="AC1Y77_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HIQ1576387.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          570 a          304 c          345 g          530 t
ORIGIN      
        1 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
       61 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
      121 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
      181 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
      241 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
      301 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
      361 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
      421 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
      481 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
      541 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
      601 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
      661 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
      721 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
      781 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
      841 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
      901 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
      961 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
     1021 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
     1081 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
     1141 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
     1201 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
     1261 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
     1321 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
     1381 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
     1441 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
     1501 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
     1561 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
     1621 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
     1681 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
     1741 aaattactt
//
LOCUS       DBNIBO010000308         1557 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.239, whole genome shotgun sequence.
ACCESSION   DBNIBO010000308 DBNIBO010000000
VERSION     DBNIBO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.239"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            <1..1142
                     /locus_tag="AC1Y77_002890"
                     /note="aph(2'')-Ia"
     CDS             <1..1142
                     /locus_tag="AC1Y77_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HIQ1576388.1"
                     /translation="GFYSLFKTLQQNRTRYIKLIFEFLKKERNANAVILDPHKNNPRA
                     IRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKV
                     DSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNI
                     EYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDIS
                     ECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFS
                     CNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDI
                     EKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1272..>1557)
                     /locus_tag="AC1Y77_002891"
     CDS             complement(1272..>1557)
                     /locus_tag="AC1Y77_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIQ1576389.1"
                     /translation="FKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLI
                     ERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          616 a          147 c          264 g          530 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg tacaagatat attaaattga
       61 tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac cctcataaaa
      121 ataatccaag agcaataagg gcataccaaa aatctggttt tagaattatt gaagatttgc
      181 cagaacatga attacacgag ggcaaaaaag aagattgtta tttaatggaa tatagatatg
      241 atgataatgc cacaaatgtt aaggcaatga aatatttaat tgagcattac tttgataatt
      301 tcaaagtaga tagtattgaa ataatcggta gtggttatga tagtgtggca tatttagtta
      361 ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaaggt tatgcaaaag
      421 aaaaagcaat atataatttt ttaaatacaa atttagaaac taatgtaaaa attcctaata
      481 ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa
      541 cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac
      601 gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat
      661 gtactattga taataaacaa aatgtattag aagagtatat attgttgcgt gaaactattt
      721 ataatgattt aactgatata gaaaaagatt atatagaaag ttttatggaa agactaaatg
      781 caacaacagt ttttgagggt aaaaagtgtt tatgccataa tgattttagt tgtaatcatc
      841 tattgttaga tggcaataat agattaactg gaataattga ttttggagat tctggaatta
      901 tagatgaata ttgtgatttt atatacttac ttgaagatag tgaagaagaa ataggaacaa
      961 attttggaga agatatatta agaatgtatg gaaatataga tattgagaaa gcaaaagaat
     1021 atcaagatat agttgaagaa tattatccta ttgaaactat tgtttatgga attaaaaata
     1081 ttaaacagga atttatcgaa aatggtagaa aagaaattta taaaaggact tataaagatt
     1141 gattatataa tatatgaaaa gctattataa aagacattag tattaaatag tttaaaaaaa
     1201 tgaaaaataa taaaggaagt gagtcaattc cagactcctg tgtaaaatgc tatacaatgt
     1261 ttttaccatt tctacttatc aaaattgatg tattttcttg aagaataaat ccattcatca
     1321 tgtaggtcca taagaacggc tccaattaag cgattggctg atgtttgatt ggggaagatg
     1381 cgaataatct tttctcttct gcgtacttct tgattcagtc gttcaattag attggtactc
     1441 tttagtcgat tgtgggaatt tccttgtacg gtatattgaa aggcgtcttc gaatccatca
     1501 tccaatgatg cgcaagcttt tgaatatttt ggttggttct gttgcaaagt tttaaat
//
LOCUS       DBNIBO010000309          950 bp    DNA     linear   BCT 21-APR-2026
DEFINITION  TPA_asm: Enterococcus faecium strain EF22974
            SAMN57369369-rid29498303.guided.275, whole genome shotgun sequence.
ACCESSION   DBNIBO010000309 DBNIBO010000000
VERSION     DBNIBO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN57369369
            Sequence Read Archive: SRR38189126
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003183836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003183836.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2026 23:57:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,892
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 24
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 40 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 25 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN57369369-rid29498303.guided.275"
                     /strain="EF22974"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.53 N 87.37 W"
                     /collection_date="2024-10-05"
     gene            112..846
                     /locus_tag="AC1Y77_002892"
     CDS             112..846
                     /locus_tag="AC1Y77_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HIQ1576390.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          387 a          125 c          129 g          309 t
ORIGIN      
        1 gtattgatga tctgtctaac ttaatttgac aattaagaca aacaaaaaat aagtggttat
       61 aatggattgt taatataaac attcattata acctcatagg agtgggttat aatgaacaaa
      121 aaaaatataa aagatagtca aaactttatt acttcaaagc atcatataaa tgaaattttg
      181 agaaatgtac atttaaatac aaatgataat attattgaga ttggttcagg gaaaggtcat
      241 ttcacgtttg aattagctaa aaggtgtaat tatgtaaccg ccattgaaat agatcctaaa
      301 ttatgtagga taactaaaaa caaacttatt gaatatgaga acttccaggt tatcaataaa
      361 gatattttac aatttaagtt tcctaaaaat aagtcatata agatatatgg aaatataccc
      421 tacaatataa gtacagatat aattcgaaaa attgtttttg aaagcacagc tacagaaagt
      481 tatttaatag tggaatatgg atttgctaaa aggttgctaa atacaaatcg ttcactagca
      541 ctatttttaa tgacagaagt tgatatatcc atattaagta aaatccctag agaatacttt
      601 catccaaaac ctagagttaa tagctcgtta attgtattaa aaagacaccc ttcaaaaata
      661 tcactcaaag atagaaaaca atatgaaaat tttgttatga aatgggttaa caaagaatac
      721 ataaaactat tttccaaaaa ccaattttat caagccttaa aatatgcaag aattgacgat
      781 ttaaacaata ttagctttga acaattcttg tctcttttca atagctataa attatttaat
      841 agataagtta aggggtgcat aaactgcatc ccttaactta tcttattttg cttaaacaat
      901 cttggcccgt aaactgcata actcatatgt aaatcgctcc tttttaggtg
//
